MD14G1017100.v1.1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002063
Physical Location & Seq
Reverse (-)
1583040 .. 1584608
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1017100.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGGACACCAATCCACTGCAGCAACAACAACCCAACATGGACAGCCATCCAATCAGCATCTGGGAAGTGAACAAGGATCGGTTGGCCACCATGTACCAGATGATCTCAGAACCCCCAAAACTCCTAAGAAATGCAGCAGGCAAACCCTCCTGCTGCATCTTCAGAGTCCCACAGAGCCTTCTCGAGATAAACGGCAAGTCGTACAACCCCCACATCGTCTCCATCGGTCCATACCACAGAGGAGAGCCACGGGTCAAGATGATGGAAGAGCACAAGTGGAAATATTTGGGTTCTTTGCTTGCAAGGACAGAGCCCAAAGGGTTGTCTTTAAAGGACTACTTGAAGGCCTTGGAGCCATTGGAACAACAAGCTAGAGAGTGTTACTCAGAAAGAATAAATCTTACCACGGAAGAATTTTTGGAAATGTTGGTTCTTGATGGTTGTTTTATAATCGAATTGTTACGAAAAGTCGGCCTTTTGGTTCGGTTCGAGCGTGACGATCCTCTTGTCAACATGGTTTGGATAATCCCATTTTTGGTTAGGGATTTTCTTAGGCTTGAAAATCAAATACCCTACTTTGTTCTCCTGGCTTTGTTTGATCTCACAACTACGGAAGAACATAAAGAAAATGGGAAGTCATTGTCGTTGCTTGCCTTGGAATTCTTCAACAACCATATGCAGAGGCCTGATCATGTCATTCAAAAGCACCACAATCTTACAGGCAAGCTCTCAAACTTTAATGATATTTCTAAAACTTTCTTTGAAATTTCTCTGTTCTGTATGCATTTGCTCGATTTGTTGCGCTCAAGTGTTATACCGCCAGGCCATGAGGAGCCACGTAGTAGCAACACCATACCAACACATACCATCCATTGTGTCTCGAAGCTTTGCCGTGCAGGAATCAAGCTCAATCCGGGCAAGGGCGAAAGCTTCTTGGTGATCAAATTCAAGCGCGGAGTCATCGAAATGCCAACTATAACAATCGACGATTTCATGAGCCCCTTCTTGCTCAACTGTGTGGCCTATGAGCAGTGCCACAAGTCAAGTTCTAAGCACATCACCACCTACGCAACATTGCTCGATTGCCTTGTGAACACATACAAGGATGTTGAGTATTTGTGTGACCGTAACATCCTCGAGAATTACTTTGGGAACGATGGGGAAGTTGCTCAATTTATTAATAACTTGGGGAAGGATGTGGCTTTTGATATGGATAGGTGTTACTTGTCAAAGTTGTTCAATGACGTGCATCACTATTATGGGAATAGTTGGCATGTTCAATGGGCTAGCTTCAAGTATACTTATTTTAATACTCCATGGTCCTTCATATCTGCGTTTGTTGCTTTGATTTTGTTGATACTCACCTTGGTACAGACCCTTTACACTGTTATGAGTTACTATCCTCCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

471

Amino Acids

54.51

Weight (kDa)

6.42

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 53 - 454 1.2e-121 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 449
AccI GTMKAC 1 cut(s) 1298
AccII CGCG 1 cut(s) 954
AciI CCGC 2 cut(s) 818, 954
AclWI GGATC 2 cut(s) 84, 494
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 4 cut(s) 413, 659, 765, 944
AcuI CTGAAG 1 cut(s) 145
AfaI GTAC 3 cut(s) 95, 203, 1371
AfiI CCNNNNNNNGG 2 cut(s) 535, 920
AgsI TTSAA 9 cut(s) 343, 560, 667, 701, 764, 949, 1240, 1280, 1294
AjiI CACGTC 1 cut(s) 1246
AjnI CCWGG 2 cut(s) 585, 820
AluBI AGCT 6 cut(s) 371, 727, 886, 907, 930, 1290
AluI AGCT 6 cut(s) 371, 727, 886, 907, 930, 1290
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 2 cut(s) 223, 883
AlwI GGATC 2 cut(s) 84, 494
AlwNI CAGNNNCTG 1 cut(s) 60
Ama87I CYCGRG 2 cut(s) 182, 1136
AoxI GGCC 6 cut(s) 84, 345, 472, 683, 823, 1020
ApeKI GCWGC 3 cut(s) 19, 134, 153
ApoI RAATTY 4 cut(s) 413, 659, 765, 944
AseI ATTAAT 1 cut(s) 1179
AspLEI GCGC 2 cut(s) 804, 954
AspS9I GGNCC 2 cut(s) 227, 1320
AsuC2I CCSGG 1 cut(s) 915
AsuHPI GGTGA 3 cut(s) 949, 1051, 1354
AsuNHI GCTAGC 1 cut(s) 1286
AvaI CYCGRG 2 cut(s) 182, 1136
AvaII GGWCC 2 cut(s) 227, 1320
BalI TGGCCA 1 cut(s) 86
BanII GRGCYC 2 cut(s) 315, 1001
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 3 cut(s) 31, 140, 146
BccI CCATC 6 cut(s) 54, 230, 256, 431, 875, 1151
BceAI ACGGC 2 cut(s) 208, 876
BciT130I CCWGG 2 cut(s) 587, 822
BclI TGATCA 2 cut(s) 688, 939
BcnI CCSGG 1 cut(s) 915
BcoDI GTCTC 2 cut(s) 223, 883
BfaI CTAG 2 cut(s) 372, 1287
BfmI CTRYAG 1 cut(s) 17
BisI GCNGC 3 cut(s) 20, 135, 154
BlsI GCNGC 3 cut(s) 21, 136, 155
Bme1390I CCNGG 3 cut(s) 587, 822, 915
Bme18I GGWCC 2 cut(s) 227, 1320
BmeT110I CYCGRG 2 cut(s) 182, 1136
BmgBI CACGTC 1 cut(s) 1246
BmgT120I GGNCC 2 cut(s) 227, 1320
BmiI GGNNCC 2 cut(s) 354, 834
BmrFI CCNGG 3 cut(s) 587, 822, 915
BmsI GCATC 3 cut(s) 66, 165, 1258
BmtI GCTAGC 1 cut(s) 1290
BplI GAGNNNNNCTC 2 cut(s) 368, 400
BpuEI CTTGAG 1 cut(s) 790
BpuMI CCSGG 1 cut(s) 915
BsaAI YACGTR 1 cut(s) 839
BsaJI CCNNGG 6 cut(s) 248, 348, 405, 654, 1316, 1365
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 535, 920
Bse3DI GCAATG 1 cut(s) 1073
BseBI CCWGG 2 cut(s) 587, 822
BseDI CCNNGG 6 cut(s) 248, 348, 405, 654, 1316, 1365
BseGI GGATG 5 cut(s) 46, 867, 1111, 1131, 1201
BseLI CCNNNNNNNGG 2 cut(s) 535, 920
BseMI GCAATG 1 cut(s) 1073
BseMII CTCAG 2 cut(s) 120, 399
BseRI GAGGAG 2 cut(s) 255, 845
BseXI GCAGC 3 cut(s) 31, 140, 146
BsgI GTGCAG 1 cut(s) 915
Bsh1236I CGCG 1 cut(s) 954
BshFI GGCC 6 cut(s) 86, 347, 474, 685, 825, 1022
BsiHKAI GWGCWC 1 cut(s) 273
BsiHKCI CYCGRG 2 cut(s) 182, 1136
BsiSI CCGG 1 cut(s) 914
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 2 cut(s) 535, 920
BsmAI GTCTC 2 cut(s) 223, 883
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 6 cut(s) 86, 347, 474, 685, 825, 1022
BsoBI CYCGRG 2 cut(s) 182, 1136
Bsp1286I GDGCHC 3 cut(s) 273, 315, 1001
Bsp143I GATC 6 cut(s) 76, 102, 499, 598, 688, 939
Bsp19I CCATGG 1 cut(s) 1316
BspACI CCGC 2 cut(s) 818, 954
BspANI GGCC 6 cut(s) 86, 347, 474, 685, 825, 1022
BspCNI CTCAG 2 cut(s) 119, 398
BspFNI CGCG 1 cut(s) 954
BspHI TCATGA 1 cut(s) 993
BspLI GGNNCC 2 cut(s) 354, 834
BspMAI CTGCAG 1 cut(s) 21
BspOI GCTAGC 1 cut(s) 1290
BspPI GGATC 2 cut(s) 84, 494
BspQI GCTCTTC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 1073
BssECI CCNNGG 6 cut(s) 248, 348, 405, 654, 1316, 1365
BssMI GATC 6 cut(s) 76, 102, 499, 598, 688, 939
BssNAI GTATAC 1 cut(s) 1299
BssT1I CCWWGG 4 cut(s) 348, 654, 1316, 1365
Bst1107I GTATAC 1 cut(s) 1299
Bst2UI CCWGG 2 cut(s) 587, 822
Bst4CI ACNGT 3 cut(s) 1016, 1127, 1387
Bst6I CTCTTC 1 cut(s) 261
BstBAI YACGTR 1 cut(s) 839
BstC8I GCNNGC 5 cut(s) 139, 300, 651, 725, 1288
BstDEI CTNAG 5 cut(s) 106, 125, 385, 551, 1050
BstDSI CCRYGG 3 cut(s) 248, 405, 1316
BstF5I GGATG 5 cut(s) 46, 867, 1111, 1131, 1201
BstFNI CGCG 1 cut(s) 954
BstHHI GCGC 2 cut(s) 804, 954
BstKTI GATC 6 cut(s) 79, 105, 502, 601, 691, 942
BstMAI GTCTC 2 cut(s) 223, 883
BstMBI GATC 6 cut(s) 76, 102, 499, 598, 688, 939
BstMWI GCNNNNNNNGC 1 cut(s) 1340
BstNI CCWGG 2 cut(s) 587, 822
BstNSI RCATGY 1 cut(s) 1277
BstSCI CCNGG 3 cut(s) 585, 820, 913
BstSFI CTRYAG 1 cut(s) 17
BstUI CGCG 1 cut(s) 954
BstV1I GCAGC 3 cut(s) 31, 140, 146
BstZ17I GTATAC 1 cut(s) 1299
BsuRI GGCC 6 cut(s) 86, 347, 474, 685, 825, 1022
BtgI CCRYGG 3 cut(s) 248, 405, 1316
BtrI CACGTC 1 cut(s) 1246
BtsCI GGATG 5 cut(s) 46, 867, 1111, 1131, 1201
BtsI GCAGTG 2 cut(s) 14, 1037
BtsIMutI CAGTG 3 cut(s) 14, 1037, 1383
Cac8I GCNNGC 5 cut(s) 139, 300, 651, 725, 1288
CaiI CAGNNNCTG 1 cut(s) 60
CciI TCATGA 1 cut(s) 993
CfoI GCGC 2 cut(s) 804, 954
Cfr13I GGNCC 2 cut(s) 227, 1320
Csp6I GTAC 3 cut(s) 94, 202, 1370
CviAII CATG 8 cut(s) 37, 91, 514, 692, 827, 994, 1274, 1317
CviQI GTAC 3 cut(s) 94, 202, 1370
DdeI CTNAG 5 cut(s) 106, 125, 385, 551, 1050
DpnI GATC 6 cut(s) 78, 104, 501, 600, 690, 941
DpnII GATC 6 cut(s) 76, 102, 499, 598, 688, 939
DraI TTTAAA 1 cut(s) 330
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco130I CCWWGG 4 cut(s) 348, 654, 1316, 1365
Eco147I AGGCCT 2 cut(s) 347, 685
Eco24I GRGCYC 2 cut(s) 315, 1001
Eco47I GGWCC 2 cut(s) 227, 1320
Eco57I CTGAAG 1 cut(s) 145
Eco88I CYCGRG 2 cut(s) 182, 1136
EcoRI GAATTC 1 cut(s) 659
EcoRII CCWGG 2 cut(s) 585, 820
EcoT14I CCWWGG 4 cut(s) 348, 654, 1316, 1365
EcoT22I ATGCAT 1 cut(s) 786
EcoT38I GRGCYC 2 cut(s) 315, 1001
ErhI CCWWGG 4 cut(s) 348, 654, 1316, 1365
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 8 cut(s) 40, 94, 517, 695, 830, 997, 1277, 1320
FalI AAGNNNNNCTT 4 cut(s) 323, 355, 459, 491
FaqI GGGAC 1 cut(s) 152
FatI CATG 8 cut(s) 36, 90, 513, 691, 826, 993, 1273, 1316
FauNDI CATATG 1 cut(s) 675
FbaI TGATCA 2 cut(s) 688, 939
FblI GTMKAC 1 cut(s) 1298
Fnu4HI GCNGC 3 cut(s) 20, 135, 154
FokI GGATG 5 cut(s) 33, 854, 1118, 1118, 1208
FriOI GRGCYC 2 cut(s) 315, 1001
Fsp4HI GCNGC 3 cut(s) 20, 135, 154
FspBI CTAG 2 cut(s) 372, 1287
GlaI GCGC 2 cut(s) 803, 953
GluI GCNGC 3 cut(s) 20, 135, 154
HaeIII GGCC 6 cut(s) 86, 347, 474, 685, 825, 1022
HapII CCGG 1 cut(s) 914
HhaI GCGC 2 cut(s) 804, 954
Hin1II CATG 8 cut(s) 40, 94, 517, 695, 830, 997, 1277, 1320
Hin6I GCGC 2 cut(s) 802, 952
HinP1I GCGC 2 cut(s) 802, 952
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 2 cut(s) 884, 928
HinfI GANTC 3 cut(s) 165, 900, 957
HpaII CCGG 1 cut(s) 914
HphI GGTGA 3 cut(s) 949, 1051, 1354
Hpy166II GTNNAC 4 cut(s) 70, 511, 1093, 1299
Hpy188I TCNGA 3 cut(s) 109, 164, 388
Hpy188III TCNNGA 7 cut(s) 182, 184, 256, 434, 880, 994, 1138
Hpy8I GTNNAC 4 cut(s) 70, 511, 1093, 1299
Hpy99I CGWCG 1 cut(s) 989
HpyAV CCTTC 5 cut(s) 188, 337, 1012, 1186, 1333
HpyCH4III ACNGT 3 cut(s) 1016, 1127, 1387
HpyCH4IV ACGT 2 cut(s) 838, 1245
HpyCH4V TGCA 8 cut(s) 19, 134, 156, 302, 679, 784, 896, 1249
HpyF10VI GCNNNNNNNGC 1 cut(s) 1340
HpyF3I CTNAG 5 cut(s) 106, 125, 385, 551, 1050
HpySE526I ACGT 2 cut(s) 838, 1245
Hsp92II CATG 8 cut(s) 40, 94, 517, 695, 830, 997, 1277, 1320
HspAI GCGC 2 cut(s) 802, 952
Ksp22I TGATCA 2 cut(s) 688, 939
Kzo9I GATC 6 cut(s) 76, 102, 499, 598, 688, 939
LguI GCTCTTC 1 cut(s) 261
LmnI GCTCC 2 cut(s) 352, 832
Lsp1109I GCAGC 3 cut(s) 31, 140, 146
LweI GCATC 3 cut(s) 66, 165, 1258
MaeI CTAG 2 cut(s) 372, 1287
MaeII ACGT 2 cut(s) 838, 1245
MaeIII GTNAC 7 cut(s) 380, 459, 494, 1121, 1127, 1220, 1394
MalI GATC 6 cut(s) 78, 104, 501, 600, 690, 941
MboI GATC 6 cut(s) 76, 102, 499, 598, 688, 939
MboII GAAGA 5 cut(s) 151, 278, 422, 626, 655
MhlI GDGCHC 3 cut(s) 273, 315, 1001
MlsI TGGCCA 1 cut(s) 86
MluCI AATT 7 cut(s) 413, 455, 659, 765, 944, 1141, 1172
MluNI TGGCCA 1 cut(s) 86
MlyI GAGTC 2 cut(s) 174, 966
MnlI CCTC 7 cut(s) 157, 233, 513, 675, 823, 1145, 1413
Mox20I TGGCCA 1 cut(s) 86
Mph1103I ATGCAT 1 cut(s) 786
MscI TGGCCA 1 cut(s) 86
MseI TTAA 4 cut(s) 329, 738, 1179, 1308
MslI CAYNNNNRTG 3 cut(s) 965, 1257, 1388
Msp20I TGGCCA 1 cut(s) 86
MspI CCGG 1 cut(s) 914
MspR9I CCNGG 3 cut(s) 587, 822, 915
MvaI CCWGG 2 cut(s) 587, 822
MvnI CGCG 1 cut(s) 954
MwoI GCNNNNNNNGC 1 cut(s) 1340
NciI CCSGG 1 cut(s) 915
NcoI CCATGG 1 cut(s) 1316
NdeI CATATG 1 cut(s) 675
NdeII GATC 6 cut(s) 76, 102, 499, 598, 688, 939
NheI GCTAGC 1 cut(s) 1286
NlaIII CATG 8 cut(s) 40, 94, 517, 695, 830, 997, 1277, 1320
NlaIV GGNNCC 2 cut(s) 354, 834
NmuCI GTSAC 2 cut(s) 494, 1121
NsiI ATGCAT 1 cut(s) 786
NspI RCATGY 1 cut(s) 1277
PaeR7I CTCGAG 2 cut(s) 182, 1136
PagI TCATGA 1 cut(s) 993
PceI AGGCCT 2 cut(s) 347, 685
PciSI GCTCTTC 1 cut(s) 261
PcsI WCGNNNNNNNCGW 2 cut(s) 490, 495
PfeI GAWTC 1 cut(s) 900
PkrI GCNGC 3 cut(s) 21, 136, 155
PleI GAGTC 2 cut(s) 173, 965
PpsI GAGTC 2 cut(s) 173, 965
Ppu21I YACGTR 1 cut(s) 839
PshBI ATTAAT 1 cut(s) 1179
PsiI TTATAA 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 585, 820
PspGI CCWGG 2 cut(s) 585, 820
PspN4I GGNNCC 2 cut(s) 354, 834
PspPI GGNCC 2 cut(s) 227, 1320
PstI CTGCAG 1 cut(s) 21
PstNI CAGNNNCTG 1 cut(s) 60
RsaI GTAC 3 cut(s) 95, 203, 1371
RsaNI GTAC 3 cut(s) 94, 202, 1370
RseI CAYNNNNRTG 3 cut(s) 965, 1257, 1388
SapI GCTCTTC 1 cut(s) 261
SaqAI TTAA 4 cut(s) 329, 738, 1179, 1308
SatI GCNGC 3 cut(s) 20, 135, 154
Sau3AI GATC 6 cut(s) 76, 102, 499, 598, 688, 939
Sau96I GGNCC 2 cut(s) 227, 1320
SchI GAGTC 2 cut(s) 174, 966
ScrFI CCNGG 3 cut(s) 587, 822, 915
SduI GDGCHC 3 cut(s) 273, 315, 1001
SfaNI GCATC 3 cut(s) 66, 165, 1258
SfcI CTRYAG 1 cut(s) 17
Sfr274I CTCGAG 2 cut(s) 182, 1136
SinI GGWCC 2 cut(s) 227, 1320
SlaI CTCGAG 2 cut(s) 182, 1136
SmiMI CAYNNNNRTG 3 cut(s) 965, 1257, 1388
SmlI CTYRAG 3 cut(s) 182, 805, 1136
SmoI CTYRAG 3 cut(s) 182, 805, 1136
Sse9I AATT 7 cut(s) 413, 455, 659, 765, 944, 1141, 1172
SseBI AGGCCT 2 cut(s) 347, 685
SsiI CCGC 2 cut(s) 818, 954
SspI AATATT 1 cut(s) 284
SspMI CTAG 2 cut(s) 372, 1287
StuI AGGCCT 2 cut(s) 347, 685
StyD4I CCNGG 3 cut(s) 585, 820, 913
StyI CCWWGG 4 cut(s) 348, 654, 1316, 1365
TaaI ACNGT 3 cut(s) 1016, 1127, 1387
TaiI ACGT 2 cut(s) 841, 1248
TaqI TCGA 9 cut(s) 183, 453, 489, 792, 881, 963, 984, 1080, 1137
TaqII GACCGA 1 cut(s) 215
TasI AATT 7 cut(s) 413, 455, 659, 765, 944, 1141, 1172
TfiI GAWTC 1 cut(s) 900
Tru1I TTAA 4 cut(s) 329, 738, 1179, 1308
Tru9I TTAA 4 cut(s) 329, 738, 1179, 1308
TscAI CASTG 3 cut(s) 21, 1037, 1390
TseFI GTSAC 2 cut(s) 494, 1121
TseI GCWGC 3 cut(s) 19, 134, 153
Tsp45I GTSAC 2 cut(s) 494, 1121
TspDTI ATGAA 2 cut(s) 982, 1315
TspGWI ACGGA 2 cut(s) 422, 626
TspRI CASTG 3 cut(s) 21, 1037, 1390
VpaK11BI GGWCC 2 cut(s) 227, 1320
VspI ATTAAT 1 cut(s) 1179
XapI RAATTY 4 cut(s) 413, 659, 765, 944
XceI RCATGY 1 cut(s) 1277
XcmI CCANNNNNNNNNTGG 1 cut(s) 57
XhoI CTCGAG 2 cut(s) 182, 1136
XmiI GTMKAC 1 cut(s) 1298
XspI CTAG 2 cut(s) 372, 1287
Zsp2I ATGCAT 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.