pycom12g01610

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002063
Physical Location & Seq
Reverse (-)
1335071 .. 1337582
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g01610.1

Sequence Viewer

Length: 1356 bp
ATGGGCCCCAATCCACCGCAACAACAGCAACAACCCAACAACAACGGCCATCCAATCAGCATCTGGGAAGCGAACCAGGATCGGTTGGGCACCATGTACCAGATGATCTCAGAACCCCCAAGACTCCTATGCAATGCAGCAGGAAAACCCTCCTGCTGCATCTTCAGAGTCCCACAGAGCCTTGTCGAGATAAACGGTAAGTCCTACAACCCTCACATCGTCTCAATCGGTCCTTACCACAGAGGAGAGCCACGTCTCAAGATGATCGAAGAGCACAAGTGGAAATACTTGGGTTCTTTGCTTGCAAGGACAGAGCCCAAAGGGTTGACTTTAGAGGACTACTTGAAGGCCTTGGAGCCATTGGAACAAAAAGCTAGAGAGTGTTACTCTGAAAGCATAAATCTTAGGACGGATGAATTTTTGGAAATGTTGGTTCTTGATAGTTGCTTTATAATCGAATTGTTTCGAAAATTCGGGCTTTTGGTTCGGTTCGAGCTTGACGATCCTCTTGTCAATATGGTTTGGATAATCCCATTTTTGGCTAGGGATTTTCTTAGGCTTGAAAATCAAATACCCTACTTTGTTCTTCTGACCTTGTTTGAGCTCACAACCATGGAAGAACATAGAGAAAATGGGAAGTCATTGTCGTTGCTTGCGTTGGAATTCTTCAACAACCATATGCAGATGCCTGATCATGTCATTCAAAAGCACCACAATCTTACAGGTATGCATTTGCTTGACTTGTTACGCTCAAGTGTTATACCCCCAGGCCATGAAGAGCCACGTAGCAGCAACACCATCCCAACACATACCATCCACTGTGTCTCGAAGCTTCGCCGTGCCGGAATCAAGCTCAATCCGGCCGAGGACGAAAGCTTCTTGGTGATCAAATTCAAGCGCGGTGTCATCGAAATGCCGCCTATAACAATCGACGATTTTATGAGCTCGTTCTTACTGAATTGTGTGGCCTATGAGCAATGCCACAAGTCAAGTTCTAAGCACATCACTACCTACGCCACATTGCTCGATTGCCTTGTGAACACATACAAGGATGTTGAGTATTTGTGTGACCGTAACATCATCGAGAATTGCTTTGGGAACGATGGGGAAGTTGCTCGATTTATTAATAATTTGGGGAAGGATGTGGCTTTTGATATGGATAGATGTTACTTGGCTAAGTTGTTCAATGATGTGCATCACTATTATGGGAATAGTTGGCATGTTCAATGGGCTAGCTTCAAGTACACTTATTTTGATACTCCATGGTCCTTCATATCTGCGTTTGCTGCTCTGATTTTGTTGATACTCACCTTGATGCAGACCTTCTACACTGTTTTGAGTTATTACCATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

452

Amino Acids

52.32

Weight (kDa)

6.02

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 54 - 436 1.6e-123 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 452
AccB1I GGYRCC 1 cut(s) 89
AccII CGCG 1 cut(s) 900
AciI CCGC 3 cut(s) 17, 900, 917
AclWI GGATC 2 cut(s) 87, 497
AcoI YGGCCR 2 cut(s) 46, 861
AcsI RAATTY 4 cut(s) 416, 470, 662, 890
AcuI CTGAAG 1 cut(s) 148
AfaI GTAC 2 cut(s) 98, 1244
AfiI CCNNNNNNNGG 1 cut(s) 538
AgsI TTSAA 8 cut(s) 346, 563, 670, 704, 895, 1186, 1226, 1240
AjiI CACGTC 1 cut(s) 254
AjnI CCWGG 2 cut(s) 75, 766
AluBI AGCT 8 cut(s) 374, 496, 604, 832, 853, 876, 945, 1236
AluI AGCT 8 cut(s) 374, 496, 604, 832, 853, 876, 945, 1236
Alw21I GWGCWC 3 cut(s) 276, 606, 947
Alw26I GTCTC 3 cut(s) 226, 260, 829
AlwI GGATC 2 cut(s) 87, 497
AlwNI CAGNNNCTG 1 cut(s) 63
AoxI GGCC 6 cut(s) 4, 46, 348, 769, 861, 966
ApaI GGGCCC 1 cut(s) 8
ApeKI GCWGC 4 cut(s) 137, 156, 789, 1286
ApoI RAATTY 4 cut(s) 416, 470, 662, 890
AseI ATTAAT 1 cut(s) 1125
Asp700I GAANNNNTTC 1 cut(s) 462
AspLEI GCGC 1 cut(s) 900
AspS9I GGNCC 4 cut(s) 4, 5, 230, 1266
AsuHPI GGTGA 2 cut(s) 895, 1300
AsuII TTCGAA 1 cut(s) 466
AsuNHI GCTAGC 1 cut(s) 1232
AvaII GGWCC 2 cut(s) 230, 1266
BaeGI GKGCMC 2 cut(s) 8, 92
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 4 cut(s) 8, 318, 606, 947
Bbv12I GWGCWC 3 cut(s) 276, 606, 947
BbvI GCAGC 4 cut(s) 143, 149, 801, 1273
BccI CCATC 4 cut(s) 57, 806, 821, 1097
BceAI ACGGC 2 cut(s) 61, 822
BciT130I CCWGG 2 cut(s) 77, 768
BclI TGATCA 2 cut(s) 691, 885
BcoDI GTCTC 3 cut(s) 226, 260, 829
BfaI CTAG 3 cut(s) 375, 543, 1233
BisI GCNGC 5 cut(s) 138, 157, 790, 917, 1287
BlsI GCNGC 5 cut(s) 139, 158, 791, 918, 1288
Bme1390I CCNGG 2 cut(s) 77, 768
Bme18I GGWCC 2 cut(s) 230, 1266
BmgBI CACGTC 1 cut(s) 254
BmgT120I GGNCC 4 cut(s) 4, 5, 230, 1266
BmiI GGNNCC 4 cut(s) 6, 7, 91, 357
BmrFI CCNGG 2 cut(s) 77, 768
BmsI GCATC 5 cut(s) 69, 168, 675, 1204, 1305
BmtI GCTAGC 1 cut(s) 1236
BplI GAGNNNNNCTC 2 cut(s) 371, 403
Bpu14I TTCGAA 1 cut(s) 466
BpuEI CTTGAG 2 cut(s) 242, 736
BsaAI YACGTR 1 cut(s) 785
BsaBI GATNNNNATC 1 cut(s) 1194
BsaJI CCNNGG 5 cut(s) 351, 612, 766, 864, 1262
Bsc4I CCNNNNNNNGG 1 cut(s) 538
Bse3DI GCAATG 3 cut(s) 139, 983, 1019
Bse8I GATNNNNATC 1 cut(s) 1194
BseBI CCWGG 2 cut(s) 77, 768
BseDI CCNNGG 5 cut(s) 351, 612, 766, 864, 1262
BseGI GGATG 6 cut(s) 49, 418, 798, 813, 1057, 1147
BseJI GATNNNNATC 1 cut(s) 1194
BseLI CCNNNNNNNGG 1 cut(s) 538
BseMI GCAATG 3 cut(s) 139, 983, 1019
BseMII CTCAG 1 cut(s) 123
BseRI GAGGAG 1 cut(s) 258
BseSI GKGCMC 2 cut(s) 8, 92
BseX3I CGGCCG 1 cut(s) 861
BseXI GCAGC 4 cut(s) 143, 149, 801, 1273
Bsh1236I CGCG 1 cut(s) 900
Bsh1285I CGRYCG 1 cut(s) 864
BshFI GGCC 6 cut(s) 6, 48, 350, 771, 863, 968
BshNI GGYRCC 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 864
BsiHKAI GWGCWC 3 cut(s) 276, 606, 947
BsiSI CCGG 2 cut(s) 843, 860
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 538
BsmAI GTCTC 3 cut(s) 226, 260, 829
BsmBI CGTCTC 2 cut(s) 226, 260
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 6 cut(s) 6, 48, 350, 771, 863, 968
Bsp119I TTCGAA 1 cut(s) 466
Bsp120I GGGCCC 1 cut(s) 4
Bsp1286I GDGCHC 6 cut(s) 8, 92, 276, 318, 606, 947
Bsp143I GATC 6 cut(s) 79, 105, 264, 502, 691, 885
Bsp19I CCATGG 2 cut(s) 612, 1262
BspACI CCGC 3 cut(s) 17, 900, 917
BspANI GGCC 6 cut(s) 6, 48, 350, 771, 863, 968
BspCNI CTCAG 1 cut(s) 122
BspFNI CGCG 1 cut(s) 900
BspLI GGNNCC 4 cut(s) 6, 7, 91, 357
BspOI GCTAGC 1 cut(s) 1236
BspPI GGATC 2 cut(s) 87, 497
BspQI GCTCTTC 2 cut(s) 264, 771
BspT104I TTCGAA 1 cut(s) 466
BspT107I GGYRCC 1 cut(s) 89
BsrDI GCAATG 3 cut(s) 139, 983, 1019
BssECI CCNNGG 5 cut(s) 351, 612, 766, 864, 1262
BssMI GATC 6 cut(s) 79, 105, 264, 502, 691, 885
BssT1I CCWWGG 3 cut(s) 351, 612, 1262
Bst2UI CCWGG 2 cut(s) 77, 768
Bst4CI ACNGT 4 cut(s) 197, 821, 1073, 1333
Bst6I CTCTTC 2 cut(s) 264, 771
BstBAI YACGTR 1 cut(s) 785
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 3 cut(s) 303, 654, 1234
BstDEI CTNAG 5 cut(s) 109, 404, 554, 996, 1176
BstDSI CCRYGG 2 cut(s) 612, 1262
BstF5I GGATG 6 cut(s) 49, 418, 798, 813, 1057, 1147
BstFNI CGCG 1 cut(s) 900
BstHHI GCGC 1 cut(s) 900
BstKTI GATC 6 cut(s) 82, 108, 267, 505, 694, 888
BstMAI GTCTC 3 cut(s) 226, 260, 829
BstMBI GATC 6 cut(s) 79, 105, 264, 502, 691, 885
BstMCI CGRYCG 1 cut(s) 864
BstMWI GCNNNNNNNGC 2 cut(s) 25, 1286
BstNI CCWGG 2 cut(s) 77, 768
BstNSI RCATGY 1 cut(s) 1223
BstSCI CCNGG 2 cut(s) 75, 766
BstSLI GKGCMC 2 cut(s) 8, 92
BstUI CGCG 1 cut(s) 900
BstV1I GCAGC 4 cut(s) 143, 149, 801, 1273
BstZI CGGCCG 1 cut(s) 861
BsuRI GGCC 6 cut(s) 6, 48, 350, 771, 863, 968
BtgI CCRYGG 2 cut(s) 612, 1262
BtrI CACGTC 1 cut(s) 254
BtsCI GGATG 6 cut(s) 49, 418, 798, 813, 1057, 1147
BtsIMutI CAGTG 2 cut(s) 817, 1329
Cac8I GCNNGC 3 cut(s) 303, 654, 1234
CaiI CAGNNNCTG 1 cut(s) 63
CfoI GCGC 1 cut(s) 900
Cfr13I GGNCC 4 cut(s) 4, 5, 230, 1266
Csp6I GTAC 2 cut(s) 97, 1243
CviAII CATG 6 cut(s) 94, 613, 695, 773, 1220, 1263
CviQI GTAC 2 cut(s) 97, 1243
DdeI CTNAG 5 cut(s) 109, 404, 554, 996, 1176
DpnI GATC 6 cut(s) 81, 107, 266, 504, 693, 887
DpnII GATC 6 cut(s) 79, 105, 264, 502, 691, 885
EaeI YGGCCR 2 cut(s) 46, 861
EagI CGGCCG 1 cut(s) 861
Eam1104I CTCTTC 2 cut(s) 264, 771
EarI CTCTTC 2 cut(s) 264, 771
Ecl136II GAGCTC 2 cut(s) 604, 945
EclXI CGGCCG 1 cut(s) 861
Eco130I CCWWGG 3 cut(s) 351, 612, 1262
Eco147I AGGCCT 1 cut(s) 350
Eco24I GRGCYC 4 cut(s) 8, 318, 606, 947
Eco47I GGWCC 2 cut(s) 230, 1266
Eco52I CGGCCG 1 cut(s) 861
Eco53kI GAGCTC 2 cut(s) 604, 945
Eco57I CTGAAG 1 cut(s) 148
EcoICRI GAGCTC 2 cut(s) 604, 945
EcoO109I RGGNCCY 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 662
EcoRII CCWGG 2 cut(s) 75, 766
EcoT14I CCWWGG 3 cut(s) 351, 612, 1262
EcoT22I ATGCAT 1 cut(s) 732
EcoT38I GRGCYC 4 cut(s) 8, 318, 606, 947
ErhI CCWWGG 3 cut(s) 351, 612, 1262
Esp3I CGTCTC 2 cut(s) 226, 260
FaeI CATG 6 cut(s) 97, 616, 698, 776, 1223, 1266
FaqI GGGAC 1 cut(s) 155
FatI CATG 6 cut(s) 93, 612, 694, 772, 1219, 1262
FauNDI CATATG 1 cut(s) 678
FbaI TGATCA 2 cut(s) 691, 885
Fnu4HI GCNGC 5 cut(s) 138, 157, 790, 917, 1287
FokI GGATG 6 cut(s) 36, 425, 785, 800, 1064, 1154
FriOI GRGCYC 4 cut(s) 8, 318, 606, 947
Fsp4HI GCNGC 5 cut(s) 138, 157, 790, 917, 1287
FspBI CTAG 3 cut(s) 375, 543, 1233
GlaI GCGC 1 cut(s) 899
GluI GCNGC 5 cut(s) 138, 157, 790, 917, 1287
HaeIII GGCC 6 cut(s) 6, 48, 350, 771, 863, 968
HapII CCGG 2 cut(s) 843, 860
HhaI GCGC 1 cut(s) 900
Hin1II CATG 6 cut(s) 97, 616, 698, 776, 1223, 1266
Hin6I GCGC 1 cut(s) 898
HinP1I GCGC 1 cut(s) 898
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HindIII AAGCTT 2 cut(s) 830, 874
HinfI GANTC 3 cut(s) 123, 168, 846
HpaII CCGG 2 cut(s) 843, 860
HphI GGTGA 2 cut(s) 895, 1300
Hpy166II GTNNAC 3 cut(s) 327, 1039, 1245
Hpy188I TCNGA 5 cut(s) 112, 167, 391, 591, 1293
Hpy188III TCNNGA 5 cut(s) 187, 259, 437, 826, 1084
Hpy8I GTNNAC 3 cut(s) 327, 1039, 1245
Hpy99I CGWCG 1 cut(s) 935
HpyAV CCTTC 4 cut(s) 340, 1132, 1279, 1333
HpyCH4III ACNGT 4 cut(s) 197, 821, 1073, 1333
HpyCH4IV ACGT 2 cut(s) 253, 784
HpyCH4V TGCA 8 cut(s) 132, 137, 159, 305, 682, 730, 1195, 1318
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 1286
HpyF3I CTNAG 5 cut(s) 109, 404, 554, 996, 1176
HpySE526I ACGT 2 cut(s) 253, 784
Hsp92II CATG 6 cut(s) 97, 616, 698, 776, 1223, 1266
HspAI GCGC 1 cut(s) 898
Ksp22I TGATCA 2 cut(s) 691, 885
Kzo9I GATC 6 cut(s) 79, 105, 264, 502, 691, 885
LguI GCTCTTC 2 cut(s) 264, 771
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 4 cut(s) 143, 149, 801, 1273
LweI GCATC 5 cut(s) 69, 168, 675, 1204, 1305
MaeI CTAG 3 cut(s) 375, 543, 1233
MaeII ACGT 2 cut(s) 253, 784
MaeIII GTNAC 5 cut(s) 383, 744, 1067, 1073, 1166
MalI GATC 6 cut(s) 81, 107, 266, 504, 693, 887
MboI GATC 6 cut(s) 79, 105, 264, 502, 691, 885
MboII GAAGA 6 cut(s) 154, 281, 578, 629, 658, 788
MhlI GDGCHC 6 cut(s) 8, 92, 276, 318, 606, 947
MluCI AATT 8 cut(s) 416, 458, 470, 662, 890, 958, 1087, 1129
MlyI GAGTC 2 cut(s) 117, 177
MmeI TCCRAC 1 cut(s) 639
MnlI CCTC 6 cut(s) 160, 222, 236, 328, 516, 859
Mph1103I ATGCAT 1 cut(s) 732
MroXI GAANNNNTTC 1 cut(s) 462
MseI TTAA 1 cut(s) 1125
MslI CAYNNNNRTG 4 cut(s) 611, 911, 1203, 1313
MspI CCGG 2 cut(s) 843, 860
MspR9I CCNGG 2 cut(s) 77, 768
MvaI CCWGG 2 cut(s) 77, 768
MvnI CGCG 1 cut(s) 900
MwoI GCNNNNNNNGC 2 cut(s) 25, 1286
NcoI CCATGG 2 cut(s) 612, 1262
NdeI CATATG 1 cut(s) 678
NdeII GATC 6 cut(s) 79, 105, 264, 502, 691, 885
NheI GCTAGC 1 cut(s) 1232
NlaIII CATG 6 cut(s) 97, 616, 698, 776, 1223, 1266
NlaIV GGNNCC 4 cut(s) 6, 7, 91, 357
NmeAIII GCCGAG 1 cut(s) 889
NmuCI GTSAC 1 cut(s) 1067
NsiI ATGCAT 1 cut(s) 732
NspI RCATGY 1 cut(s) 1223
NspV TTCGAA 1 cut(s) 466
PceI AGGCCT 1 cut(s) 350
PciSI GCTCTTC 2 cut(s) 264, 771
PcsI WCGNNNNNNNCGW 2 cut(s) 498, 653
PdmI GAANNNNTTC 1 cut(s) 462
PfeI GAWTC 1 cut(s) 846
PkrI GCNGC 5 cut(s) 139, 158, 791, 918, 1288
PleI GAGTC 2 cut(s) 117, 176
PpsI GAGTC 2 cut(s) 117, 176
Ppu21I YACGTR 1 cut(s) 785
PshBI ATTAAT 1 cut(s) 1125
PsiI TTATAA 1 cut(s) 452
Psp124BI GAGCTC 2 cut(s) 606, 947
Psp6I CCWGG 2 cut(s) 75, 766
PspGI CCWGG 2 cut(s) 75, 766
PspN4I GGNNCC 4 cut(s) 6, 7, 91, 357
PspOMI GGGCCC 1 cut(s) 4
PspPI GGNCC 4 cut(s) 4, 5, 230, 1266
PstNI CAGNNNCTG 1 cut(s) 63
RsaI GTAC 2 cut(s) 98, 1244
RsaNI GTAC 2 cut(s) 97, 1243
RseI CAYNNNNRTG 4 cut(s) 611, 911, 1203, 1313
SacI GAGCTC 2 cut(s) 606, 947
SapI GCTCTTC 2 cut(s) 264, 771
SaqAI TTAA 1 cut(s) 1125
SatI GCNGC 5 cut(s) 138, 157, 790, 917, 1287
Sau3AI GATC 6 cut(s) 79, 105, 264, 502, 691, 885
Sau96I GGNCC 4 cut(s) 4, 5, 230, 1266
SchI GAGTC 2 cut(s) 117, 177
ScrFI CCNGG 2 cut(s) 77, 768
SduI GDGCHC 6 cut(s) 8, 92, 276, 318, 606, 947
SfaNI GCATC 5 cut(s) 69, 168, 675, 1204, 1305
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 2 cut(s) 230, 1266
SmiMI CAYNNNNRTG 4 cut(s) 611, 911, 1203, 1313
SmlI CTYRAG 2 cut(s) 257, 751
SmoI CTYRAG 2 cut(s) 257, 751
Sse9I AATT 8 cut(s) 416, 458, 470, 662, 890, 958, 1087, 1129
SseBI AGGCCT 1 cut(s) 350
SsiI CCGC 3 cut(s) 17, 900, 917
SspMI CTAG 3 cut(s) 375, 543, 1233
SstI GAGCTC 2 cut(s) 606, 947
StuI AGGCCT 1 cut(s) 350
StyD4I CCNGG 2 cut(s) 75, 766
StyI CCWWGG 3 cut(s) 351, 612, 1262
TaaI ACNGT 4 cut(s) 197, 821, 1073, 1333
TaiI ACGT 2 cut(s) 256, 787
TaqII GACCGA 1 cut(s) 218
TasI AATT 8 cut(s) 416, 458, 470, 662, 890, 958, 1087, 1129
TatI WGTACW 1 cut(s) 1242
TauI GCSGC 1 cut(s) 919
TfiI GAWTC 1 cut(s) 846
Tru1I TTAA 1 cut(s) 1125
Tru9I TTAA 1 cut(s) 1125
TscAI CASTG 2 cut(s) 824, 1336
TseFI GTSAC 1 cut(s) 1067
TseI GCWGC 4 cut(s) 137, 156, 789, 1286
Tsp45I GTSAC 1 cut(s) 1067
TspDTI ATGAA 3 cut(s) 429, 789, 1261
TspGWI ACGGA 1 cut(s) 425
TspRI CASTG 2 cut(s) 824, 1336
VpaK11BI GGWCC 2 cut(s) 230, 1266
VspI ATTAAT 1 cut(s) 1125
XapI RAATTY 4 cut(s) 416, 470, 662, 890
XceI RCATGY 1 cut(s) 1223
XcmI CCANNNNNNNNNTGG 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 3 cut(s) 375, 543, 1233
Zsp2I ATGCAT 1 cut(s) 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.