Rh3DG173200

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002063
Physical Location & Seq
Forward (+)
15216452 .. 15218738
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG173200.1

Sequence Viewer

Length: 1344 bp
ATGGATACCAATCCAATTCAAGAAGAACACGACCATGTCAACCCCACCTGGGAAATGAGCAGTGATCGCTTAGCTTCCATGTACCAGAAGATAGCAGACCCTCCACGACTCCTCAGCAACGCGGCCGGCAAAGCCTCCTGCTGCATCTTCCGAGTCCCGCAGAGCCTCCTCGAGATAAACGGCAAGGCTTACCAGCCCCACATCGTCTCCATCGGCCCCTACCACCGCGGCGAGCCCCGCCTCAGAATGATCGAGGAGCACAAGTGGCGCTACCTGGGCTCGCTGCTTTCAAGAACAGAGCCCAAAGGGTTGACTCTACAGCACTACTTGAAGTCCATAGAGCCATTGGAAGCTAGAGCCAGGGAGTGTTACTCTGAGACCATCCATCTCAGCACTGATGATTTCCTAGAAATGATGGTTGTTGATGGTTGCTTCTTAATCGAGTTGTTTCGAAAAGTTGGGCGTGTGGTTCGGTTTGAGCGTGATGATCCACTCATTAATATGTTTTGGATACTACCGTTTATGGTCAGGGATTTTCTTCGACTTGAGAACCAAATGCCCTACTTTATTCTTGAGCACTTGTATGATCTGATAAACAAGGCCGAAGATCGTAAAGACTCTGTGAAATCCTTGTCCTTGCTTGCTTTGCAATTCTTCAACATCCATTTGATGAGGCCTGACCCCGTCATAGAAAAGCTCCATAGTCTTACGGGTATGCATTTGCTTGACTTGTTGCGGTCAAGTGTGATACCAGCGGGTTATGCAGAGCCACAAAGAAGAAACAGTACACCAACGCACATAATACATTGTGTGTCTAAGCTTCGTCGCGCTGGTATCAAGCTCAATGTTAAGGAATTCGAAAGCTTCTTGGTGGTCAAATTCAAGCGTGGAGCGATCGAGATGCCATCCATCACAATCGACGATTTCATGTGCGCTTTCTTGTTGAACTGCGTGGCCTATGAGCAGTGCCACAAGTCATGCTCGAAACACATCACCACTTATGCCACATTCGTCGATTGCCTCGTGAACAGTTACAAGGATGTCGAGTATTTGTGTGACAGAAACATAATGGAGAATTGCTATGGGAACGACGGGGAAATTGCTCGTTTCATTAACAATATGGGGAAGGATGTGGCTTTTGATTATGATAGGTGTTATCTGTCGAAATTGTTCAATGATGTTCATCAGTATTACCGGAATAGTTGGCATGTTCAATGGGCTAGCTTCAAGTACAGATACTTTGACACACCGTGGTCATTTGTTTCGGCCTTTGCTGCTTTAATTTTGCTCATACTCACAGTGCTGCAAACCGTATACTCTGCTTTAAGTTATTATTATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

447

Amino Acids

52.27

Weight (kDa)

6.78

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 49 - 432 2.3e-120 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1314
AccII CGCG 3 cut(s) 122, 228, 828
AciI CCGC 7 cut(s) 122, 158, 226, 228, 238, 736, 755
AclWI GGATC 1 cut(s) 482
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 2 cut(s) 854, 878
AdeI CACNNNGTG 1 cut(s) 1251
AfaI GTAC 3 cut(s) 83, 787, 1232
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 9 cut(s) 20, 291, 331, 658, 883, 946, 1174, 1214, 1228
AjnI CCWGG 3 cut(s) 47, 273, 359
AluBI AGCT 7 cut(s) 74, 353, 697, 820, 841, 864, 1224
AluI AGCT 7 cut(s) 74, 353, 697, 820, 841, 864, 1224
Alw21I GWGCWC 2 cut(s) 261, 579
Alw26I GTCTC 2 cut(s) 211, 371
AlwI GGATC 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 6 cut(s) 123, 214, 600, 674, 954, 1266
ApeKI GCWGC 4 cut(s) 141, 283, 1274, 1303
ApoI RAATTY 2 cut(s) 854, 878
AseI ATTAAT 1 cut(s) 498
Asp700I GAANNNNTTC 1 cut(s) 1169
AspLEI GCGC 3 cut(s) 270, 830, 935
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 1 cut(s) 985
AsuII TTCGAA 2 cut(s) 451, 858
AsuNHI GCTAGC 1 cut(s) 1220
AvaI CYCGRG 1 cut(s) 170
BaeI ACNNNNGTAYC 2 cut(s) 503, 536
BanII GRGCYC 3 cut(s) 237, 281, 303
BarI GAAGNNNNNNTAC 2 cut(s) 769, 801
BauI CACGAG 1 cut(s) 1022
Bbv12I GWGCWC 2 cut(s) 261, 579
BbvCI CCTCAGC 1 cut(s) 113
BbvI GCAGC 4 cut(s) 128, 270, 1261, 1290
BccI CCATC 7 cut(s) 218, 389, 393, 409, 419, 913, 917
BceAI ACGGC 1 cut(s) 196
BcgI CGANNNNNNTGC 2 cut(s) 883, 917
BciT130I CCWGG 3 cut(s) 49, 275, 361
BciVI GTATCC 1 cut(s) 504
BcoDI GTCTC 2 cut(s) 211, 371
BfaI CTAG 3 cut(s) 354, 407, 1221
BfmI CTRYAG 1 cut(s) 317
BfoI RGCGCY 1 cut(s) 271
BfuI GTATCC 1 cut(s) 504
BisI GCNGC 6 cut(s) 123, 142, 229, 284, 1275, 1304
BlpI GCTNAGC 1 cut(s) 70
BlsI GCNGC 6 cut(s) 124, 143, 230, 285, 1276, 1305
Bme1390I CCNGG 3 cut(s) 49, 275, 361
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 3 cut(s) 49, 275, 361
BmsI GCATC 2 cut(s) 153, 891
BmtI GCTAGC 1 cut(s) 1224
BplI GAGNNNNNCTC 2 cut(s) 356, 388
Bpu10I CCTNAGC 1 cut(s) 113
Bpu1102I GCTNAGC 1 cut(s) 70
Bpu14I TTCGAA 2 cut(s) 451, 858
BpuEI CTTGAG 2 cut(s) 566, 593
BsaBI GATNNNNATC 2 cut(s) 9, 1182
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 5 cut(s) 48, 226, 274, 360, 1250
BsaWI WCCGGW 1 cut(s) 1194
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 248, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse118I RCCGGY 1 cut(s) 125
Bse8I GATNNNNATC 2 cut(s) 9, 1182
BseBI CCWGG 3 cut(s) 49, 275, 361
BseDI CCNNGG 5 cut(s) 48, 226, 274, 360, 1250
BseGI GGATG 5 cut(s) 381, 660, 905, 1045, 1135
BseJI GATNNNNATC 2 cut(s) 9, 1182
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMII CTCAG 4 cut(s) 127, 256, 366, 403
BseRI GAGGAG 3 cut(s) 101, 158, 269
BseX3I CGGCCG 1 cut(s) 123
BseXI GCAGC 4 cut(s) 128, 270, 1261, 1290
Bsh1236I CGCG 3 cut(s) 122, 228, 828
Bsh1285I CGRYCG 2 cut(s) 126, 897
BshFI GGCC 6 cut(s) 125, 216, 602, 676, 956, 1268
BsiEI CGRYCG 2 cut(s) 126, 897
BsiHKAI GWGCWC 2 cut(s) 261, 579
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 2 cut(s) 126, 1195
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 2 cut(s) 211, 371
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 6 cut(s) 125, 216, 602, 676, 956, 1268
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 170
Bsp119I TTCGAA 2 cut(s) 451, 858
Bsp1286I GDGCHC 5 cut(s) 237, 261, 281, 303, 579
Bsp143I GATC 6 cut(s) 64, 249, 487, 586, 607, 894
Bsp1720I GCTNAGC 1 cut(s) 70
BspACI CCGC 7 cut(s) 122, 158, 226, 228, 238, 736, 755
BspANI GGCC 6 cut(s) 125, 216, 602, 676, 956, 1268
BspCNI CTCAG 4 cut(s) 126, 255, 367, 402
BspFNI CGCG 3 cut(s) 122, 228, 828
BspLI GGNNCC 1 cut(s) 217
BspOI GCTAGC 1 cut(s) 1224
BspPI GGATC 1 cut(s) 482
BspT104I TTCGAA 2 cut(s) 451, 858
BspTNI GGTCTC 1 cut(s) 371
BsrFI RCCGGY 1 cut(s) 125
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 5 cut(s) 48, 226, 274, 360, 1250
BssMI GATC 6 cut(s) 64, 249, 487, 586, 607, 894
BssNAI GTATAC 1 cut(s) 1315
BssSI CACGAG 1 cut(s) 1022
Bst1107I GTATAC 1 cut(s) 1315
Bst2BI CACGAG 1 cut(s) 1022
Bst2UI CCWGG 3 cut(s) 49, 275, 361
Bst4CI ACNGT 6 cut(s) 519, 785, 1031, 1251, 1300, 1312
BstBI TTCGAA 2 cut(s) 451, 858
BstC8I GCNNGC 5 cut(s) 127, 233, 281, 642, 1222
BstDEI CTNAG 6 cut(s) 70, 113, 242, 375, 389, 816
BstDSI CCRYGG 2 cut(s) 226, 1250
BstF5I GGATG 5 cut(s) 381, 660, 905, 1045, 1135
BstFNI CGCG 3 cut(s) 122, 228, 828
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 3 cut(s) 270, 830, 935
BstKTI GATC 6 cut(s) 67, 252, 490, 589, 610, 897
BstMAI GTCTC 2 cut(s) 211, 371
BstMBI GATC 6 cut(s) 64, 249, 487, 586, 607, 894
BstMCI CGRYCG 2 cut(s) 126, 897
BstMWI GCNNNNNNNGC 8 cut(s) 66, 131, 237, 265, 276, 646, 761, 1274
BstNI CCWGG 3 cut(s) 49, 275, 361
BstNSI RCATGY 1 cut(s) 1211
BstSCI CCNGG 3 cut(s) 47, 273, 359
BstSFI CTRYAG 1 cut(s) 317
BstUI CGCG 3 cut(s) 122, 228, 828
BstV1I GCAGC 4 cut(s) 128, 270, 1261, 1290
BstZ17I GTATAC 1 cut(s) 1315
BstZI CGGCCG 1 cut(s) 123
BsuI GTATCC 1 cut(s) 504
BsuRI GGCC 6 cut(s) 125, 216, 602, 676, 956, 1268
BtgI CCRYGG 2 cut(s) 226, 1250
BtsCI GGATG 5 cut(s) 381, 660, 905, 1045, 1135
BtsI GCAGTG 2 cut(s) 67, 971
BtsIMutI CAGTG 4 cut(s) 67, 393, 971, 1305
Cac8I GCNNGC 5 cut(s) 127, 233, 281, 642, 1222
CfoI GCGC 3 cut(s) 270, 830, 935
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 215
Cfr42I CCGCGG 1 cut(s) 229
Csp6I GTAC 3 cut(s) 82, 786, 1231
CviAII CATG 5 cut(s) 35, 79, 928, 978, 1208
CviQI GTAC 3 cut(s) 82, 786, 1231
DdeI CTNAG 6 cut(s) 70, 113, 242, 375, 389, 816
DpnI GATC 6 cut(s) 66, 251, 489, 588, 609, 896
DpnII GATC 6 cut(s) 64, 249, 487, 586, 607, 894
DraIII CACNNNGTG 1 cut(s) 1251
EaeI YGGCCR 1 cut(s) 123
EagI CGGCCG 1 cut(s) 123
EclXI CGGCCG 1 cut(s) 123
Eco147I AGGCCT 1 cut(s) 676
Eco24I GRGCYC 3 cut(s) 237, 281, 303
Eco31I GGTCTC 1 cut(s) 371
Eco52I CGGCCG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 170
EcoRI GAATTC 1 cut(s) 854
EcoRII CCWGG 3 cut(s) 47, 273, 359
EcoT22I ATGCAT 1 cut(s) 720
EcoT38I GRGCYC 3 cut(s) 237, 281, 303
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 5 cut(s) 38, 82, 931, 981, 1211
FaqI GGGAC 1 cut(s) 140
FatI CATG 5 cut(s) 34, 78, 927, 977, 1207
FauI CCCGC 3 cut(s) 165, 245, 748
FblI GTMKAC 1 cut(s) 1314
Fnu4HI GCNGC 6 cut(s) 123, 142, 229, 284, 1275, 1304
FokI GGATG 5 cut(s) 368, 647, 892, 1052, 1142
FriOI GRGCYC 3 cut(s) 237, 281, 303
Fsp4HI GCNGC 6 cut(s) 123, 142, 229, 284, 1275, 1304
FspBI CTAG 3 cut(s) 354, 407, 1221
GlaI GCGC 3 cut(s) 269, 829, 934
GluI GCNGC 6 cut(s) 123, 142, 229, 284, 1275, 1304
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 6 cut(s) 125, 216, 602, 676, 956, 1268
HapII CCGG 2 cut(s) 126, 1195
HhaI GCGC 3 cut(s) 270, 830, 935
Hin1II CATG 5 cut(s) 38, 82, 931, 981, 1211
Hin6I GCGC 3 cut(s) 268, 828, 933
HinP1I GCGC 3 cut(s) 268, 828, 933
HincII GTYRAC 2 cut(s) 40, 312
HindII GTYRAC 2 cut(s) 40, 312
HindIII AAGCTT 2 cut(s) 818, 862
HinfI GANTC 4 cut(s) 108, 153, 313, 617
HpaII CCGG 2 cut(s) 126, 1195
HphI GGTGA 1 cut(s) 985
Hpy166II GTNNAC 5 cut(s) 40, 312, 788, 1027, 1315
Hpy188I TCNGA 4 cut(s) 152, 245, 376, 591
Hpy188III TCNNGA 7 cut(s) 20, 172, 291, 572, 898, 1024, 1341
Hpy8I GTNNAC 5 cut(s) 40, 312, 788, 1027, 1315
Hpy99I CGWCG 4 cut(s) 828, 923, 1016, 1094
HpyAV CCTTC 1 cut(s) 1120
HpyCH4III ACNGT 6 cut(s) 519, 785, 1031, 1251, 1300, 1312
HpyCH4V TGCA 5 cut(s) 144, 649, 718, 764, 1306
HpyF10VI GCNNNNNNNGC 8 cut(s) 66, 131, 237, 265, 276, 646, 761, 1274
HpyF3I CTNAG 6 cut(s) 70, 113, 242, 375, 389, 816
Hsp92II CATG 5 cut(s) 38, 82, 931, 981, 1211
HspAI GCGC 3 cut(s) 268, 828, 933
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 6 cut(s) 64, 249, 487, 586, 607, 894
LmnI GCTCC 3 cut(s) 256, 702, 890
Lsp1109I GCAGC 4 cut(s) 128, 270, 1261, 1290
LweI GCATC 2 cut(s) 153, 891
MaeI CTAG 3 cut(s) 354, 407, 1221
MaeIII GTNAC 3 cut(s) 368, 1031, 1055
MalI GATC 6 cut(s) 66, 251, 489, 588, 609, 896
MboI GATC 6 cut(s) 64, 249, 487, 586, 607, 894
MboII GAAGA 7 cut(s) 35, 100, 139, 530, 617, 646, 789
MhlI GDGCHC 5 cut(s) 237, 261, 281, 303, 579
MluCI AATT 8 cut(s) 15, 650, 854, 878, 1075, 1098, 1166, 1281
MlyI GAGTC 4 cut(s) 102, 162, 307, 611
MnlI CCTC 9 cut(s) 111, 122, 145, 176, 179, 247, 251, 666, 1031
Mph1103I ATGCAT 1 cut(s) 720
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 1 cut(s) 1169
MseI TTAA 6 cut(s) 437, 498, 849, 1113, 1280, 1325
MslI CAYNNNNRTG 3 cut(s) 33, 500, 582
MspA1I CMGCKG 2 cut(s) 228, 755
MspI CCGG 2 cut(s) 126, 1195
MspR9I CCNGG 3 cut(s) 49, 275, 361
MvaI CCWGG 3 cut(s) 49, 275, 361
MvnI CGCG 3 cut(s) 122, 228, 828
MwoI GCNNNNNNNGC 8 cut(s) 66, 131, 237, 265, 276, 646, 761, 1274
NaeI GCCGGC 1 cut(s) 127
NdeII GATC 6 cut(s) 64, 249, 487, 586, 607, 894
NgoMIV GCCGGC 1 cut(s) 125
NheI GCTAGC 1 cut(s) 1220
NlaIII CATG 5 cut(s) 38, 82, 931, 981, 1211
NlaIV GGNNCC 1 cut(s) 217
NmuCI GTSAC 1 cut(s) 1055
NsiI ATGCAT 1 cut(s) 720
NspI RCATGY 1 cut(s) 1211
NspV TTCGAA 2 cut(s) 451, 858
PaeR7I CTCGAG 1 cut(s) 170
PceI AGGCCT 1 cut(s) 676
PcsI WCGNNNNNNNCGW 2 cut(s) 478, 1020
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 1 cut(s) 1169
PflFI GACNNNGTC 2 cut(s) 35, 683
PkrI GCNGC 6 cut(s) 124, 143, 230, 285, 1276, 1305
Ple19I CGATCG 1 cut(s) 897
PleI GAGTC 4 cut(s) 102, 161, 307, 611
PpsI GAGTC 4 cut(s) 102, 161, 307, 611
PshBI ATTAAT 1 cut(s) 498
Psp6I CCWGG 3 cut(s) 47, 273, 359
PspGI CCWGG 3 cut(s) 47, 273, 359
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 215
PsyI GACNNNGTC 2 cut(s) 35, 683
PvuI CGATCG 1 cut(s) 897
RsaI GTAC 3 cut(s) 83, 787, 1232
RsaNI GTAC 3 cut(s) 82, 786, 1231
RseI CAYNNNNRTG 3 cut(s) 33, 500, 582
SacII CCGCGG 1 cut(s) 229
SaqAI TTAA 6 cut(s) 437, 498, 849, 1113, 1280, 1325
SatI GCNGC 6 cut(s) 123, 142, 229, 284, 1275, 1304
Sau3AI GATC 6 cut(s) 64, 249, 487, 586, 607, 894
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 4 cut(s) 102, 162, 307, 611
ScrFI CCNGG 3 cut(s) 49, 275, 361
SduI GDGCHC 5 cut(s) 237, 261, 281, 303, 579
SfaNI GCATC 2 cut(s) 153, 891
SfcI CTRYAG 1 cut(s) 317
Sfr274I CTCGAG 1 cut(s) 170
Sfr303I CCGCGG 1 cut(s) 229
SfuI TTCGAA 2 cut(s) 451, 858
SgrBI CCGCGG 1 cut(s) 229
SlaI CTCGAG 1 cut(s) 170
SmiMI CAYNNNNRTG 3 cut(s) 33, 500, 582
SmlI CTYRAG 3 cut(s) 170, 545, 572
SmoI CTYRAG 3 cut(s) 170, 545, 572
Sse9I AATT 8 cut(s) 15, 650, 854, 878, 1075, 1098, 1166, 1281
SseBI AGGCCT 1 cut(s) 676
SsiI CCGC 7 cut(s) 122, 158, 226, 228, 238, 736, 755
SspMI CTAG 3 cut(s) 354, 407, 1221
StuI AGGCCT 1 cut(s) 676
StyD4I CCNGG 3 cut(s) 47, 273, 359
TaaI ACNGT 6 cut(s) 519, 785, 1031, 1251, 1300, 1312
TasI AATT 8 cut(s) 15, 650, 854, 878, 1075, 1098, 1166, 1281
TatI WGTACW 2 cut(s) 785, 1230
TauI GCSGC 2 cut(s) 125, 231
Tru1I TTAA 6 cut(s) 437, 498, 849, 1113, 1280, 1325
Tru9I TTAA 6 cut(s) 437, 498, 849, 1113, 1280, 1325
TscAI CASTG 4 cut(s) 67, 400, 971, 1305
TseFI GTSAC 1 cut(s) 1055
TseI GCWGC 4 cut(s) 141, 283, 1274, 1303
Tsp45I GTSAC 1 cut(s) 1055
TspDTI ATGAA 3 cut(s) 916, 1099, 1172
TspRI CASTG 4 cut(s) 67, 400, 971, 1305
Tth111I GACNNNGTC 2 cut(s) 35, 683
VspI ATTAAT 1 cut(s) 498
XapI RAATTY 2 cut(s) 854, 878
XceI RCATGY 1 cut(s) 1211
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 170
XmiI GTMKAC 1 cut(s) 1314
XmnI GAANNNNTTC 1 cut(s) 1169
XspI CTAG 3 cut(s) 354, 407, 1221
Zsp2I ATGCAT 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.