Rw3G016500

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002063
Physical Location & Seq
Forward (+)
17518289 .. 17521850
3562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G016500.1

Sequence Viewer

Length: 1707 bp
ATGGATACCAATCCAGTTCAAGAAGAACACAACCATGTCAATCCCACCTGGGAAATGAGCAGTGATCGCTTAGCTTCCATGTACCAGAAGATAGCAGACCCTCCACGACTCCTCAGCAACGCGGCCGGCAAAGCCTCCTGCTGCATCTTCCGAGTCCCGCAGAGCCTCCTCGAGATAAACGGCAAGGCTTACCAGCCGCACATCGTCTCCATTGGCCCCTACCACCGCGGCGAGCCCCGCCTCAGAATGATCGAGGAGCACAAGTGGCGCTACCTGGGCTCGCTGCTTTCAAGAACAGAGCCCAAAGGGTTGACTCTACAGCACTACTTGAAGTCCATAGAGCCATTGGAAGCTAGAGCCAGGGAGTGTTACTCTGAGACCATCCATCTCAGCACTGATGAGTTCCTAGAAATGATGGTTGTTGATGGTTGCTTCTTAATCGAGTTGTTTCAAAAAGTTGGGCGTGTGGTTCGGTTTGAGCGTGATGATCCACTCATTAATATGTTTTGGATACTACCGTTTATGGTCAGGGATTTTCTTCGACTTGAGAACCAAATGCCCTACTTTATTCTTGAGCACTTGTATGATCTGATAAACAAGGCCGAAGATCGTAAAGACTCTGTGAAATCCTTGTCCTTGCTTGCTTTGCAATTCTTCAACATCCATTTGATGAGGCCTGACCCCGTCATAGAAAAGCTCCATAGTCTTACAGGTATGCATTTGCTTGACTTGTTGCGGTCAAGTGTGATACCAGTGGATTATGCAGAGCCACAAAGAAGAAACAGTACACCAACCCACATAATACATTGTGTGTCTAAGCTTCGTCGCGCTGGTATCAAGCTCAATGTTAAGGAATTCGAAAGCTTCTTGGTGGTCAAATTCAAGCGTGGAGCGATCGAGATGCCATCCATCACAATCGACGATTTCATGTGCGCTTTCTTGTTGAACTGCGTGGCCTATGAGCAGTGCCACAAGTCATGCTCGAAACACATCACCACTTATGCCACATTCCTCGATTGCCTCGTGAACAGTTACAAGGATGTCGAGTATTTGTGTGACAGAAACATAATGGAGAATTGCTATGGGAACGATGGGGAAATTGCTCGTTTCATTAACAATATGGGGAAGGATGTGGCTTTTGATTATGATAGGTGTTATCTGTCGAAATTGTTCAATGATGTTCATCAGTATTACCGGAATAGTTGGCATGTTCAATGGGCTAGCTTCAAAATGAGTTCAGAAACCTGTGCATGCCAAAATTCAGGGGTGAATCAACTTCCTAGACCCACAAGGCCGATAAGCAGCTACGGCATGGCTACGATGTCTTCATTGCCTTCATCTTTTCCTCATCCCACAAGACCAGTTAGCATAGACCAATACAATGCTCAACAAGTTGTTTATGATGAAACTTTTGTCCATGGGGCACAACTAACTGAAGAGGAAGATAGGGAAGGTCTTGATGGATTGCTTGGGTTCATGTCAGAGCTTATTGAAAAAGTTCCTGCAAAACTAGAACACATATTACTTGATAAATATATGGATCATAGAGAAAATGAAGGTCCTGTTATTGTGGAAAGGGGTACTACTACTACTACTAGTCGCGGTCTTCAAGGAACTGGGGAATCATCACCAAAGCTTGAGCGCTTCAAGATCCGAACTGTGGATTTTGGGGATGATGTCAAGGAGGAAGAGAACGTGAAGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

568

Amino Acids

65.64

Weight (kDa)

5.98

Isoelectric Point (pI)

54.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 49 - 410 6.6e-111 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 122, 228, 828, 1602
AciI CCGC 8 cut(s) 122, 158, 197, 226, 228, 238, 736, 1602
AclWI GGATC 3 cut(s) 482, 1548, 1645
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 3 cut(s) 854, 878, 1258
AcuI CTGAAG 1 cut(s) 1455
AfaI GTAC 3 cut(s) 83, 787, 1582
AfeI AGCGCT 1 cut(s) 1643
AfiI CCNNNNNNNGG 1 cut(s) 1660
AhlI ACTAGT 1 cut(s) 1595
AjnI CCWGG 3 cut(s) 47, 273, 359
Alw21I GWGCWC 2 cut(s) 261, 579
Alw26I GTCTC 2 cut(s) 211, 371
AlwI GGATC 3 cut(s) 482, 1548, 1645
Ama87I CYCGRG 1 cut(s) 170
Aor51HI AGCGCT 1 cut(s) 1643
AoxI GGCC 6 cut(s) 123, 214, 600, 674, 954, 1292
ApeKI GCWGC 3 cut(s) 141, 283, 1302
ApoI RAATTY 3 cut(s) 854, 878, 1258
AseI ATTAAT 1 cut(s) 498
Asp700I GAANNNNTTC 2 cut(s) 1169, 1497
AspLEI GCGC 4 cut(s) 270, 830, 935, 1644
AspS9I GGNCC 2 cut(s) 215, 1559
AsuHPI GGTGA 3 cut(s) 985, 1279, 1620
AsuII TTCGAA 1 cut(s) 858
AsuNHI GCTAGC 1 cut(s) 1220
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 1 cut(s) 1559
BaeGI GKGCMC 1 cut(s) 1426
BaeI ACNNNNGTAYC 2 cut(s) 503, 536
BanII GRGCYC 3 cut(s) 237, 281, 303
BarI GAAGNNNNNNTAC 2 cut(s) 769, 801
BauI CACGAG 1 cut(s) 1022
BbsI GAAGAC 2 cut(s) 1317, 1598
Bbv12I GWGCWC 2 cut(s) 261, 579
BbvCI CCTCAGC 1 cut(s) 113
BbvI GCAGC 3 cut(s) 128, 270, 1314
BccI CCATC 8 cut(s) 389, 393, 409, 419, 913, 917, 1085, 1454
BceAI ACGGC 2 cut(s) 196, 1324
BcgI CGANNNNNNTGC 2 cut(s) 883, 917
BciT130I CCWGG 3 cut(s) 49, 275, 361
BciVI GTATCC 1 cut(s) 504
BcoDI GTCTC 2 cut(s) 211, 371
BcuI ACTAGT 1 cut(s) 1595
BfaI CTAG 6 cut(s) 354, 407, 1221, 1281, 1511, 1596
BfmI CTRYAG 1 cut(s) 317
BfoI RGCGCY 2 cut(s) 271, 1645
BfuI GTATCC 1 cut(s) 504
BisI GCNGC 6 cut(s) 123, 142, 197, 229, 284, 1303
BlpI GCTNAGC 1 cut(s) 70
BlsI GCNGC 6 cut(s) 124, 143, 198, 230, 285, 1304
Bme1390I CCNGG 3 cut(s) 49, 275, 361
Bme18I GGWCC 1 cut(s) 1559
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 215, 1559
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 3 cut(s) 49, 275, 361
BmrI ACTGGG 1 cut(s) 1626
BmsI GCATC 2 cut(s) 153, 891
BmtI GCTAGC 1 cut(s) 1224
BmuI ACTGGG 1 cut(s) 1626
BpiI GAAGAC 2 cut(s) 1317, 1598
BplI GAGNNNNNCTC 2 cut(s) 356, 388
Bpu10I CCTNAGC 1 cut(s) 113
Bpu1102I GCTNAGC 1 cut(s) 70
Bpu14I TTCGAA 1 cut(s) 858
BpuEI CTTGAG 3 cut(s) 566, 593, 1658
BsaBI GATNNNNATC 2 cut(s) 9, 1182
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 5 cut(s) 48, 226, 274, 360, 1417
BsaWI WCCGGW 1 cut(s) 1194
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 248, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 1660
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 4 cut(s) 14, 752, 1361, 1621
Bse3DI GCAATG 1 cut(s) 1328
Bse8I GATNNNNATC 2 cut(s) 9, 1182
BseBI CCWGG 3 cut(s) 49, 275, 361
BseDI CCNNGG 5 cut(s) 48, 226, 274, 360, 1417
BseGI GGATG 7 cut(s) 381, 660, 905, 1045, 1135, 1348, 1678
BseJI GATNNNNATC 2 cut(s) 9, 1182
BseLI CCNNNNNNNGG 1 cut(s) 1660
BseMI GCAATG 1 cut(s) 1328
BseMII CTCAG 4 cut(s) 127, 256, 366, 403
BseNI ACTGG 4 cut(s) 14, 752, 1361, 1621
BseRI GAGGAG 3 cut(s) 101, 158, 269
BseSI GKGCMC 1 cut(s) 1426
BseX3I CGGCCG 1 cut(s) 123
BseXI GCAGC 3 cut(s) 128, 270, 1314
Bsh1236I CGCG 4 cut(s) 122, 228, 828, 1602
Bsh1285I CGRYCG 2 cut(s) 126, 897
BshFI GGCC 6 cut(s) 125, 216, 602, 676, 956, 1294
BsiEI CGRYCG 2 cut(s) 126, 897
BsiHKAI GWGCWC 2 cut(s) 261, 579
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 2 cut(s) 126, 1195
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 1660
BsmAI GTCTC 2 cut(s) 211, 371
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 6 cut(s) 125, 216, 602, 676, 956, 1294
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 858
Bsp1286I GDGCHC 6 cut(s) 237, 261, 281, 303, 579, 1426
Bsp143I GATC 8 cut(s) 64, 249, 487, 586, 607, 894, 1540, 1650
Bsp1720I GCTNAGC 1 cut(s) 70
Bsp19I CCATGG 1 cut(s) 1417
BspACI CCGC 8 cut(s) 122, 158, 197, 226, 228, 238, 736, 1602
BspANI GGCC 6 cut(s) 125, 216, 602, 676, 956, 1294
BspCNI CTCAG 4 cut(s) 126, 255, 367, 402
BspFNI CGCG 4 cut(s) 122, 228, 828, 1602
BspLI GGNNCC 1 cut(s) 217
BspOI GCTAGC 1 cut(s) 1224
BspPI GGATC 3 cut(s) 482, 1548, 1645
BspT104I TTCGAA 1 cut(s) 858
BspTNI GGTCTC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 1328
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 4 cut(s) 14, 752, 1361, 1621
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 5 cut(s) 48, 226, 274, 360, 1417
BssMI GATC 8 cut(s) 64, 249, 487, 586, 607, 894, 1540, 1650
BssSI CACGAG 1 cut(s) 1022
BssT1I CCWWGG 1 cut(s) 1417
Bst2BI CACGAG 1 cut(s) 1022
Bst2UI CCWGG 3 cut(s) 49, 275, 361
Bst4CI ACNGT 4 cut(s) 519, 785, 1031, 1660
Bst6I CTCTTC 2 cut(s) 1431, 1683
BstBI TTCGAA 1 cut(s) 858
BstC8I GCNNGC 6 cut(s) 127, 233, 281, 642, 1222, 1252
BstDEI CTNAG 6 cut(s) 70, 113, 242, 375, 389, 816
BstDSI CCRYGG 2 cut(s) 226, 1417
BstF5I GGATG 7 cut(s) 381, 660, 905, 1045, 1135, 1348, 1678
BstFNI CGCG 4 cut(s) 122, 228, 828, 1602
BstH2I RGCGCY 2 cut(s) 271, 1645
BstHHI GCGC 4 cut(s) 270, 830, 935, 1644
BstKTI GATC 8 cut(s) 67, 252, 490, 589, 610, 897, 1543, 1653
BstMAI GTCTC 2 cut(s) 211, 371
BstMBI GATC 8 cut(s) 64, 249, 487, 586, 607, 894, 1540, 1650
BstMCI CGRYCG 2 cut(s) 126, 897
BstMWI GCNNNNNNNGC 7 cut(s) 66, 131, 237, 265, 276, 646, 1308
BstNI CCWGG 3 cut(s) 49, 275, 361
BstNSI RCATGY 2 cut(s) 1211, 1254
BstSCI CCNGG 3 cut(s) 47, 273, 359
BstSFI CTRYAG 1 cut(s) 317
BstSLI GKGCMC 1 cut(s) 1426
BstUI CGCG 4 cut(s) 122, 228, 828, 1602
BstV1I GCAGC 3 cut(s) 128, 270, 1314
BstV2I GAAGAC 2 cut(s) 1317, 1598
BstX2I RGATCY 1 cut(s) 1650
BstYI RGATCY 1 cut(s) 1650
BstZI CGGCCG 1 cut(s) 123
BsuI GTATCC 1 cut(s) 504
BsuRI GGCC 6 cut(s) 125, 216, 602, 676, 956, 1294
BtgI CCRYGG 2 cut(s) 226, 1417
BtsCI GGATG 7 cut(s) 381, 660, 905, 1045, 1135, 1348, 1678
BtsI GCAGTG 2 cut(s) 67, 971
BtsIMutI CAGTG 4 cut(s) 67, 393, 759, 971
Cac8I GCNNGC 6 cut(s) 127, 233, 281, 642, 1222, 1252
CfoI GCGC 4 cut(s) 270, 830, 935, 1644
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 215, 1559
Cfr42I CCGCGG 1 cut(s) 229
Csp6I GTAC 3 cut(s) 82, 786, 1581
CviAII CATG 9 cut(s) 35, 79, 928, 978, 1208, 1251, 1312, 1418, 1477
CviQI GTAC 3 cut(s) 82, 786, 1581
DdeI CTNAG 6 cut(s) 70, 113, 242, 375, 389, 816
DpnI GATC 8 cut(s) 66, 251, 489, 588, 609, 896, 1542, 1652
DpnII GATC 8 cut(s) 64, 249, 487, 586, 607, 894, 1540, 1650
EaeI YGGCCR 1 cut(s) 123
EagI CGGCCG 1 cut(s) 123
Eam1104I CTCTTC 2 cut(s) 1431, 1683
EarI CTCTTC 2 cut(s) 1431, 1683
EclXI CGGCCG 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 1417
Eco147I AGGCCT 1 cut(s) 676
Eco24I GRGCYC 3 cut(s) 237, 281, 303
Eco31I GGTCTC 1 cut(s) 371
Eco47I GGWCC 1 cut(s) 1559
Eco47III AGCGCT 1 cut(s) 1643
Eco52I CGGCCG 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 1455
Eco88I CYCGRG 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 1559
EcoRI GAATTC 1 cut(s) 854
EcoRII CCWGG 3 cut(s) 47, 273, 359
EcoT14I CCWWGG 1 cut(s) 1417
EcoT22I ATGCAT 1 cut(s) 720
EcoT38I GRGCYC 3 cut(s) 237, 281, 303
ErhI CCWWGG 1 cut(s) 1417
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 9 cut(s) 38, 82, 931, 981, 1211, 1254, 1315, 1421, 1480
FaqI GGGAC 1 cut(s) 140
FatI CATG 9 cut(s) 34, 78, 927, 977, 1207, 1250, 1311, 1417, 1476
FauI CCCGC 2 cut(s) 165, 245
Fnu4HI GCNGC 6 cut(s) 123, 142, 197, 229, 284, 1303
FokI GGATG 7 cut(s) 368, 647, 892, 1052, 1142, 1335, 1685
FriOI GRGCYC 3 cut(s) 237, 281, 303
Fsp4HI GCNGC 6 cut(s) 123, 142, 197, 229, 284, 1303
FspBI CTAG 6 cut(s) 354, 407, 1221, 1281, 1511, 1596
GlaI GCGC 4 cut(s) 269, 829, 934, 1643
GluI GCNGC 6 cut(s) 123, 142, 197, 229, 284, 1303
HaeII RGCGCY 2 cut(s) 271, 1645
HaeIII GGCC 6 cut(s) 125, 216, 602, 676, 956, 1294
HapII CCGG 2 cut(s) 126, 1195
HhaI GCGC 4 cut(s) 270, 830, 935, 1644
Hin1II CATG 9 cut(s) 38, 82, 931, 981, 1211, 1254, 1315, 1421, 1480
Hin6I GCGC 4 cut(s) 268, 828, 933, 1642
HinP1I GCGC 4 cut(s) 268, 828, 933, 1642
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HindIII AAGCTT 3 cut(s) 818, 862, 1634
HinfI GANTC 6 cut(s) 108, 153, 313, 617, 1270, 1622
HpaII CCGG 2 cut(s) 126, 1195
HphI GGTGA 3 cut(s) 985, 1279, 1620
Hpy166II GTNNAC 3 cut(s) 312, 788, 1027
Hpy188I TCNGA 7 cut(s) 152, 245, 376, 591, 1240, 1483, 1655
Hpy188III TCNNGA 8 cut(s) 20, 172, 291, 572, 898, 1024, 1457, 1648
Hpy8I GTNNAC 3 cut(s) 312, 788, 1027
Hpy99I CGWCG 2 cut(s) 828, 923
HpyAV CCTTC 4 cut(s) 1120, 1344, 1445, 1550
HpyCH4III ACNGT 4 cut(s) 519, 785, 1031, 1660
HpyCH4IV ACGT 1 cut(s) 1695
HpyCH4V TGCA 6 cut(s) 144, 649, 718, 764, 1250, 1505
HpyF10VI GCNNNNNNNGC 7 cut(s) 66, 131, 237, 265, 276, 646, 1308
HpyF3I CTNAG 6 cut(s) 70, 113, 242, 375, 389, 816
HpySE526I ACGT 1 cut(s) 1695
Hsp92II CATG 9 cut(s) 38, 82, 931, 981, 1211, 1254, 1315, 1421, 1480
HspAI GCGC 4 cut(s) 268, 828, 933, 1642
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 8 cut(s) 64, 249, 487, 586, 607, 894, 1540, 1650
LmnI GCTCC 3 cut(s) 256, 702, 890
Lsp1109I GCAGC 3 cut(s) 128, 270, 1314
LweI GCATC 2 cut(s) 153, 891
MaeI CTAG 6 cut(s) 354, 407, 1221, 1281, 1511, 1596
MaeII ACGT 1 cut(s) 1695
MaeIII GTNAC 3 cut(s) 368, 1031, 1055
MalI GATC 8 cut(s) 66, 251, 489, 588, 609, 896, 1542, 1652
MboI GATC 8 cut(s) 64, 249, 487, 586, 607, 894, 1540, 1650
MflI RGATCY 1 cut(s) 1650
MhlI GDGCHC 6 cut(s) 237, 261, 281, 303, 579, 1426
MluCI AATT 7 cut(s) 650, 854, 878, 1075, 1098, 1166, 1258
MlyI GAGTC 4 cut(s) 102, 162, 307, 611
Mph1103I ATGCAT 1 cut(s) 720
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 2 cut(s) 1169, 1497
MseI TTAA 4 cut(s) 437, 498, 849, 1113
MslI CAYNNNNRTG 3 cut(s) 33, 500, 582
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 2 cut(s) 126, 1195
MspR9I CCNGG 3 cut(s) 49, 275, 361
MvaI CCWGG 3 cut(s) 49, 275, 361
MvnI CGCG 4 cut(s) 122, 228, 828, 1602
MwoI GCNNNNNNNGC 7 cut(s) 66, 131, 237, 265, 276, 646, 1308
NaeI GCCGGC 1 cut(s) 127
NcoI CCATGG 1 cut(s) 1417
NdeII GATC 8 cut(s) 64, 249, 487, 586, 607, 894, 1540, 1650
NgoMIV GCCGGC 1 cut(s) 125
NheI GCTAGC 1 cut(s) 1220
NlaIII CATG 9 cut(s) 38, 82, 931, 981, 1211, 1254, 1315, 1421, 1480
NlaIV GGNNCC 1 cut(s) 217
NmuCI GTSAC 1 cut(s) 1055
NsiI ATGCAT 1 cut(s) 720
NspI RCATGY 2 cut(s) 1211, 1254
NspV TTCGAA 1 cut(s) 858
PaeI GCATGC 1 cut(s) 1254
PaeR7I CTCGAG 1 cut(s) 170
PceI AGGCCT 1 cut(s) 676
PcsI WCGNNNNNNNCGW 2 cut(s) 478, 1020
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 2 cut(s) 1169, 1497
PfeI GAWTC 2 cut(s) 1270, 1622
PflFI GACNNNGTC 1 cut(s) 683
PkrI GCNGC 6 cut(s) 124, 143, 198, 230, 285, 1304
Ple19I CGATCG 1 cut(s) 897
PleI GAGTC 4 cut(s) 102, 161, 307, 611
PpsI GAGTC 4 cut(s) 102, 161, 307, 611
PpuMI RGGWCCY 1 cut(s) 1559
PshBI ATTAAT 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 1559
Psp6I CCWGG 3 cut(s) 47, 273, 359
PspGI CCWGG 3 cut(s) 47, 273, 359
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 2 cut(s) 215, 1559
PspPPI RGGWCCY 1 cut(s) 1559
PsrI GAACNNNNNNTAC 2 cut(s) 1506, 1538
PsuI RGATCY 1 cut(s) 1650
PsyI GACNNNGTC 1 cut(s) 683
PvuI CGATCG 1 cut(s) 897
RsaI GTAC 3 cut(s) 83, 787, 1582
RsaNI GTAC 3 cut(s) 82, 786, 1581
RseI CAYNNNNRTG 3 cut(s) 33, 500, 582
SacII CCGCGG 1 cut(s) 229
SaqAI TTAA 4 cut(s) 437, 498, 849, 1113
SatI GCNGC 6 cut(s) 123, 142, 197, 229, 284, 1303
Sau3AI GATC 8 cut(s) 64, 249, 487, 586, 607, 894, 1540, 1650
Sau96I GGNCC 2 cut(s) 215, 1559
SchI GAGTC 4 cut(s) 102, 162, 307, 611
ScrFI CCNGG 3 cut(s) 49, 275, 361
SduI GDGCHC 6 cut(s) 237, 261, 281, 303, 579, 1426
SfaNI GCATC 2 cut(s) 153, 891
SfcI CTRYAG 1 cut(s) 317
Sfr274I CTCGAG 1 cut(s) 170
Sfr303I CCGCGG 1 cut(s) 229
SfuI TTCGAA 1 cut(s) 858
SgrBI CCGCGG 1 cut(s) 229
SinI GGWCC 1 cut(s) 1559
SlaI CTCGAG 1 cut(s) 170
SmiMI CAYNNNNRTG 3 cut(s) 33, 500, 582
SmlI CTYRAG 4 cut(s) 170, 545, 572, 1637
SmoI CTYRAG 4 cut(s) 170, 545, 572, 1637
SpeI ACTAGT 1 cut(s) 1595
SphI GCATGC 1 cut(s) 1254
Sse9I AATT 7 cut(s) 650, 854, 878, 1075, 1098, 1166, 1258
SseBI AGGCCT 1 cut(s) 676
SsiI CCGC 8 cut(s) 122, 158, 197, 226, 228, 238, 736, 1602
SspMI CTAG 6 cut(s) 354, 407, 1221, 1281, 1511, 1596
StuI AGGCCT 1 cut(s) 676
StyD4I CCNGG 3 cut(s) 47, 273, 359
StyI CCWWGG 1 cut(s) 1417
TaaI ACNGT 4 cut(s) 519, 785, 1031, 1660
TaiI ACGT 1 cut(s) 1698
TasI AATT 7 cut(s) 650, 854, 878, 1075, 1098, 1166, 1258
TatI WGTACW 1 cut(s) 785
TauI GCSGC 3 cut(s) 125, 199, 231
TfiI GAWTC 2 cut(s) 1270, 1622
Tru1I TTAA 4 cut(s) 437, 498, 849, 1113
Tru9I TTAA 4 cut(s) 437, 498, 849, 1113
TscAI CASTG 4 cut(s) 67, 400, 759, 971
TseFI GTSAC 1 cut(s) 1055
TseI GCWGC 3 cut(s) 141, 283, 1302
Tsp45I GTSAC 1 cut(s) 1055
TspDTI ATGAA 8 cut(s) 916, 1099, 1172, 1317, 1326, 1419, 1465, 1569
TspRI CASTG 4 cut(s) 67, 400, 759, 971
Tth111I GACNNNGTC 1 cut(s) 683
VpaK11BI GGWCC 1 cut(s) 1559
VspI ATTAAT 1 cut(s) 498
XapI RAATTY 3 cut(s) 854, 878, 1258
XceI RCATGY 2 cut(s) 1211, 1254
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 170
XmnI GAANNNNTTC 2 cut(s) 1169, 1497
XspI CTAG 6 cut(s) 354, 407, 1221, 1281, 1511, 1596
Zsp2I ATGCAT 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.