Rorug03G0124000

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002063
Physical Location & Seq
Forward (+)
10023028 .. 10024186
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0124000.1

Sequence Viewer

Length: 930 bp
ATGTGTCCGCCACCAAATGAAGGACAAATAATAGCACAAGAATCCAATTCAGAAATCCAAGCCAACTACAATTTCCTTTCCCATTACCCATCAACCCCAGTTCCCTTTCTATTTCATTGCCTCTTCATTCCACACACACACACAGTCATGGCCTCATCGGATCAGACCTTCTTCAACAACTCCCTCCTAGCTCTCTACCTCATAGGCCCTCCCACCTTCATCTCCCTCAGATTCCTCCAAGCCCCATATGGCAAGCACAACCGTCCAGGGTGGGGCCCCACCATGTCTCCGCCGCTGGCTTGGTTCCTCATGGAATCACCAACCCTTTGGCTCACTCTACTCCTCTTCCCCTATGGCCAACACAGTTCCAACCCCCAAGCCTTAATTCTAATGACCCCCTTTCTTTTCCACTATTTCAACCGAACCTGCCTCTACCCACTTCGCCTCTACCGGAATACCACCCGGAAAACCACCCCCGGTTTTCCGGTCAGCGTGGCGTCGATGGCATTTGTGTTCAATCTGTTGAATGGTTATTTGCAATCCAGATGGGTGTCTCACTACAAGGACTATGAGAGTGATCATGGGTGGTTCTGGTGTAGGTTTTTTGTTGGGTTGGTTGTGTTTTTGTGTGGGATGGGTGTGAATGTGTGGTCTGATAGGGTTTTAGTGGGTTTGAAAAAAGAGGGTGGAGGGTATAAGATCCCCAAAGGAGGGTGGTTTGAGTTGGTGAGCTGCCCTAACTACTTTGGGGAAATAGTGGAGTGGATAGGATGGGCAGTGATGACTTGGTCATGGGCAGGACTAGGGTTTATGCTATACACGTGTGCCAATTTGGTGCCCAGGGCACGTGCGAACCATAGGTGGTATTTGGAGAAGTTTGGGGAGGACTATCCCAAGTCCAGAAAAGCTGTTATCCCTTTCTTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

309

Amino Acids

35.66

Weight (kDa)

9.01

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Steroid_dh PF02544 161 - 309 1.5e-56 3-oxo-5-alpha-steroid 4-dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 434
AccB1I GGYRCC 1 cut(s) 835
AciI CCGC 3 cut(s) 8, 290, 293
AclWI GGATC 2 cut(s) 168, 694
AcoI YGGCCR 1 cut(s) 355
AcvI CACGTG 2 cut(s) 822, 848
AcyI GRCGYC 1 cut(s) 497
AfiI CCNNNNNNNGG 4 cut(s) 20, 272, 710, 711
AflIII ACRYGT 2 cut(s) 819, 821
AgsI TTSAA 5 cut(s) 175, 418, 517, 526, 676
AjnI CCWGG 2 cut(s) 265, 839
AjuI GAANNNNNNNTTGG 4 cut(s) 56, 88, 369, 401
AluBI AGCT 3 cut(s) 191, 732, 908
AluI AGCT 3 cut(s) 191, 732, 908
Alw26I GTCTC 2 cut(s) 291, 558
AlwI GGATC 2 cut(s) 168, 694
AoxI GGCC 4 cut(s) 150, 205, 274, 355
ApaI GGGCCC 1 cut(s) 278
ApeKI GCWGC 1 cut(s) 732
AspS9I GGNCC 3 cut(s) 206, 274, 275
AsuC2I CCSGG 2 cut(s) 463, 477
AsuHPI GGTGA 2 cut(s) 309, 739
BaeGI GKGCMC 3 cut(s) 278, 840, 847
BalI TGGCCA 1 cut(s) 357
BanI GGYRCC 1 cut(s) 835
BanII GRGCYC 1 cut(s) 278
BbrPI CACGTG 2 cut(s) 822, 848
BbvI GCAGC 1 cut(s) 719
BccI CCATC 5 cut(s) 97, 496, 540, 628, 765
BciT130I CCWGG 2 cut(s) 267, 841
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 2 cut(s) 463, 477
BcoDI GTCTC 2 cut(s) 291, 558
BfaI CTAG 2 cut(s) 188, 803
BfuAI ACCTGC 1 cut(s) 434
BisI GCNGC 2 cut(s) 293, 733
BlsI GCNGC 2 cut(s) 294, 734
Bme1390I CCNGG 4 cut(s) 267, 463, 477, 841
BmgT120I GGNCC 3 cut(s) 206, 274, 275
BmiI GGNNCC 5 cut(s) 275, 276, 277, 305, 837
BmrFI CCNGG 4 cut(s) 267, 463, 477, 841
BmrI ACTGGG 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 92
BpuMI CCSGG 2 cut(s) 463, 477
BsaAI YACGTR 2 cut(s) 822, 848
BsaHI GRCGYC 1 cut(s) 497
BsaJI CCNNGG 4 cut(s) 266, 475, 839, 840
BsaWI WCCGGW 2 cut(s) 450, 484
BsaXI ACNNNNNCTCC 2 cut(s) 271, 301
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 272, 710, 711
Bse1I ACTGG 1 cut(s) 98
Bse3DI GCAATG 1 cut(s) 115
BseBI CCWGG 2 cut(s) 267, 841
BseDI CCNNGG 4 cut(s) 266, 475, 839, 840
BseGI GGATG 2 cut(s) 639, 776
BseLI CCNNNNNNNGG 4 cut(s) 20, 272, 710, 711
BseMI GCAATG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 241
BseNI ACTGG 1 cut(s) 98
BseRI GAGGAG 1 cut(s) 332
BseSI GKGCMC 3 cut(s) 278, 840, 847
BseXI GCAGC 1 cut(s) 719
BshFI GGCC 4 cut(s) 152, 207, 276, 357
BshNI GGYRCC 1 cut(s) 835
BsiSI CCGG 4 cut(s) 451, 463, 477, 485
BslI CCNNNNNNNGG 4 cut(s) 20, 272, 710, 711
BsmAI GTCTC 2 cut(s) 291, 558
BsnI GGCC 4 cut(s) 152, 207, 276, 357
Bsp120I GGGCCC 1 cut(s) 274
Bsp1286I GDGCHC 3 cut(s) 278, 840, 847
Bsp143I GATC 3 cut(s) 160, 577, 699
BspACI CCGC 3 cut(s) 8, 290, 293
BspANI GGCC 4 cut(s) 152, 207, 276, 357
BspCNI CTCAG 1 cut(s) 240
BspLI GGNNCC 5 cut(s) 275, 276, 277, 305, 837
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 2 cut(s) 168, 694
BspT107I GGYRCC 1 cut(s) 835
BsrDI GCAATG 1 cut(s) 115
BsrI ACTGG 1 cut(s) 98
BssECI CCNNGG 4 cut(s) 266, 475, 839, 840
BssMI GATC 3 cut(s) 160, 577, 699
BssNI GRCGYC 1 cut(s) 497
Bst2UI CCWGG 2 cut(s) 267, 841
Bst4CI ACNGT 3 cut(s) 145, 263, 365
Bst6I CTCTTC 2 cut(s) 128, 350
BstACI GRCGYC 1 cut(s) 497
BstBAI YACGTR 2 cut(s) 822, 848
BstC8I GCNNGC 2 cut(s) 254, 297
BstDEI CTNAG 1 cut(s) 227
BstF5I GGATG 2 cut(s) 639, 776
BstKTI GATC 3 cut(s) 163, 580, 702
BstMAI GTCTC 2 cut(s) 291, 558
BstMBI GATC 3 cut(s) 160, 577, 699
BstMWI GCNNNNNNNGC 1 cut(s) 503
BstNI CCWGG 2 cut(s) 267, 841
BstSCI CCNGG 4 cut(s) 265, 461, 475, 839
BstSLI GKGCMC 3 cut(s) 278, 840, 847
BstV1I GCAGC 1 cut(s) 719
BstX2I RGATCY 1 cut(s) 699
BstXI CCANNNNNNTGG 1 cut(s) 327
BstYI RGATCY 1 cut(s) 699
BsuRI GGCC 4 cut(s) 152, 207, 276, 357
BtsCI GGATG 2 cut(s) 639, 776
BtsI GCAGTG 1 cut(s) 783
BtsIMutI CAGTG 1 cut(s) 783
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 2 cut(s) 254, 297
Cfr13I GGNCC 3 cut(s) 206, 274, 275
CseI GACGC 1 cut(s) 486
CviAII CATG 5 cut(s) 148, 283, 310, 581, 792
DdeI CTNAG 1 cut(s) 227
DpnI GATC 3 cut(s) 162, 579, 701
DpnII GATC 3 cut(s) 160, 577, 699
EaeI YGGCCR 1 cut(s) 355
Eam1104I CTCTTC 2 cut(s) 128, 350
EarI CTCTTC 2 cut(s) 128, 350
EciI GGCGGA 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 278
Eco72I CACGTG 2 cut(s) 822, 848
EcoO109I RGGNCCY 3 cut(s) 206, 274, 275
EcoRII CCWGG 2 cut(s) 265, 839
EcoT38I GRGCYC 1 cut(s) 278
FaeI CATG 5 cut(s) 151, 286, 313, 584, 795
FatI CATG 5 cut(s) 147, 282, 309, 580, 791
FauNDI CATATG 1 cut(s) 247
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 2 cut(s) 293, 733
FokI GGATG 2 cut(s) 646, 783
FriOI GRGCYC 1 cut(s) 278
Fsp4HI GCNGC 2 cut(s) 293, 733
FspBI CTAG 2 cut(s) 188, 803
GluI GCNGC 2 cut(s) 293, 733
HaeIII GGCC 4 cut(s) 152, 207, 276, 357
HapII CCGG 4 cut(s) 451, 463, 477, 485
HgaI GACGC 1 cut(s) 486
Hin1I GRCGYC 1 cut(s) 497
Hin1II CATG 5 cut(s) 151, 286, 313, 584, 795
HinfI GANTC 3 cut(s) 41, 231, 314
HpaII CCGG 4 cut(s) 451, 463, 477, 485
HphI GGTGA 2 cut(s) 309, 739
Hpy188I TCNGA 5 cut(s) 52, 160, 165, 230, 655
Hpy188III TCNNGA 2 cut(s) 543, 900
Hpy99I CGWCG 1 cut(s) 502
HpyAV CCTTC 3 cut(s) 14, 178, 226
HpyCH4III ACNGT 3 cut(s) 145, 263, 365
HpyCH4IV ACGT 2 cut(s) 821, 847
HpyCH4V TGCA 1 cut(s) 538
HpyF10VI GCNNNNNNNGC 1 cut(s) 503
HpyF3I CTNAG 1 cut(s) 227
HpySE526I ACGT 2 cut(s) 821, 847
Hsp92I GRCGYC 1 cut(s) 497
Hsp92II CATG 5 cut(s) 151, 286, 313, 584, 795
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 3 cut(s) 160, 577, 699
Lsp1109I GCAGC 1 cut(s) 719
MaeI CTAG 2 cut(s) 188, 803
MaeII ACGT 2 cut(s) 821, 847
MalI GATC 3 cut(s) 162, 579, 701
MboI GATC 3 cut(s) 160, 577, 699
MboII GAAGA 3 cut(s) 115, 163, 337
MflI RGATCY 1 cut(s) 699
MhlI GDGCHC 3 cut(s) 278, 840, 847
MlsI TGGCCA 1 cut(s) 357
MluCI AATT 4 cut(s) 46, 70, 384, 829
MluNI TGGCCA 1 cut(s) 357
MmeI TCCRAC 1 cut(s) 393
Mox20I TGGCCA 1 cut(s) 357
MscI TGGCCA 1 cut(s) 357
MseI TTAA 1 cut(s) 383
MslI CAYNNNNRTG 1 cut(s) 146
Msp20I TGGCCA 1 cut(s) 357
MspA1I CMGCKG 1 cut(s) 295
MspI CCGG 4 cut(s) 451, 463, 477, 485
MspR9I CCNGG 4 cut(s) 267, 463, 477, 841
MvaI CCWGG 2 cut(s) 267, 841
MwoI GCNNNNNNNGC 1 cut(s) 503
NciI CCSGG 2 cut(s) 463, 477
NdeI CATATG 1 cut(s) 247
NdeII GATC 3 cut(s) 160, 577, 699
NlaIII CATG 5 cut(s) 151, 286, 313, 584, 795
NlaIV GGNNCC 5 cut(s) 275, 276, 277, 305, 837
PasI CCCWGGG 1 cut(s) 840
PfeI GAWTC 3 cut(s) 41, 231, 314
PflFI GACNNNGTC 1 cut(s) 787
PkrI GCNGC 2 cut(s) 294, 734
PmaCI CACGTG 2 cut(s) 822, 848
PmlI CACGTG 2 cut(s) 822, 848
Ppu21I YACGTR 2 cut(s) 822, 848
Psp6I CCWGG 2 cut(s) 265, 839
PspCI CACGTG 2 cut(s) 822, 848
PspGI CCWGG 2 cut(s) 265, 839
PspN4I GGNNCC 5 cut(s) 275, 276, 277, 305, 837
PspOMI GGGCCC 1 cut(s) 274
PspPI GGNCC 3 cut(s) 206, 274, 275
PsuI RGATCY 1 cut(s) 699
PsyI GACNNNGTC 1 cut(s) 787
RseI CAYNNNNRTG 1 cut(s) 146
SaqAI TTAA 1 cut(s) 383
SatI GCNGC 2 cut(s) 293, 733
Sau3AI GATC 3 cut(s) 160, 577, 699
Sau96I GGNCC 3 cut(s) 206, 274, 275
ScrFI CCNGG 4 cut(s) 267, 463, 477, 841
SduI GDGCHC 3 cut(s) 278, 840, 847
SmiMI CAYNNNNRTG 1 cut(s) 146
Sse9I AATT 4 cut(s) 46, 70, 384, 829
SsiI CCGC 3 cut(s) 8, 290, 293
SspMI CTAG 2 cut(s) 188, 803
StyD4I CCNGG 4 cut(s) 265, 461, 475, 839
TaaI ACNGT 3 cut(s) 145, 263, 365
TaiI ACGT 2 cut(s) 824, 850
TaqI TCGA 1 cut(s) 500
TasI AATT 4 cut(s) 46, 70, 384, 829
TauI GCSGC 1 cut(s) 295
TfiI GAWTC 3 cut(s) 41, 231, 314
Tru1I TTAA 1 cut(s) 383
Tru9I TTAA 1 cut(s) 383
TscAI CASTG 1 cut(s) 783
TseI GCWGC 1 cut(s) 732
TspDTI ATGAA 4 cut(s) 33, 104, 115, 208
TspRI CASTG 1 cut(s) 783
Tth111I GACNNNGTC 1 cut(s) 787
XcmI CCANNNNNNNNNTGG 1 cut(s) 245
XspI CTAG 2 cut(s) 188, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.