Rh3BG202200

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002063
Physical Location & Seq
Reverse (-)
18015505 .. 18016929
1425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG202200.1

Sequence Viewer

Length: 1293 bp
ATGAACAATGATCGCCTAGCTTCCATGTACAAGAAGATCGCAGACCCTCCGAGACTCCTCAGCAATGCTGCCGGCAAAGACTCCTGCTGCATCTTCCGAGTCCCGCAGAGTCTCCTCGAGATTAACGGCAAGTCGTACCAGCCCCACATCGTCTCCATCGGTCCCTTCCACAGCGGCCAGCCCCACCTCAGAATGATCGAGGAGCACAAGTGGCGTTACCTGGGCTCTCTGCTTTCAAGAACAGAGCCCAAAGGGTTGACTCTCCAGCACTACTTGAAGTCCATAGAGCCAATGGAAGATAGAGTCAGAGAGTGTTACTCTGAAACAATCCACCTCAGTAGTGATGAGTTCATCGAAATGATGGTTGTTGATGGTTGCTTCTTAATCGAGTTGTTTCGAAAATTTGACCGTGTGGTTCGGTTTGAGCGCGATGATCCACTCATCAATATGTTCTGGGTAATTCCGTTTATGGTTAGAGATTTTCTTCGTCTTGAGAACCAAATCCCCTACTTTATACTTGAGCATTTGTATGATCTGATAACCAAGTCGGAAGATCGTCAAGACTCTGTGAAGTCTTTGTCCTTGCTTGCTTTAAACTTCTTCAACAACCATATGGACAGGCCGAAAGAAGTCATTGCAAAGCTGCATAATCTTACAGGCATGCATTTGCTTGACTTACTCCGGTCAAGTGTGATACCAGCCGACCATGTTGAACCAGGACACAAGAATAGTACCCCAACCCACATTATACATTGTATTTCAAAGCTTCGTCGCGCTGGAATCAAGCTCAATCCTCCTAAAGAATACGAAAGCTTCTTGGCGATCAAGTTCAAGCGTGGAGTTATCGAAATGCCCCCAATCATCATAGACGATTTCATGAGCTCGTTCTTGTTGAACTGTGTAGCCTATGAGCAATGCCACAAGTCATGCTCCAAACACATCACCGCTTACGCAACGTTGCTGGATTACCTAGTGAATACTTACAAGGATGTCGAGTATTTGGCTGACAGAAACATAATCGAGAATTGCTTCGGAAACGATGAGGAGGTTGCTCATTTTATTAACAATTTGGGTAAGGATGTGGCTTTTGATGATGAAAGATGTTATCTGTCGAAATTGTTCAATGATGTTCATCAGTATTACAGCAATAGTAGGCATGTTCAATGGGCAAGCTTCAAGTACACTTACTTTGACACTCCATGGTCGTTTATTTCGGCTTTTGCTGCGTTGATTTTGCTCATATTCACCTTGCTGCAGACCTTATACACTGTTTTAAGTTATTATCATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

430

Amino Acids

50.26

Weight (kDa)

6.37

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 31 - 415 5e-124 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 429, 774
AciI CCGC 3 cut(s) 104, 174, 945
AclI AACGTT 1 cut(s) 956
AclWI GGATC 1 cut(s) 428
AcoI YGGCCR 1 cut(s) 175
AcsI RAATTY 1 cut(s) 401
AfaI GTAC 4 cut(s) 29, 137, 733, 1181
AjnI CCWGG 2 cut(s) 219, 715
AluBI AGCT 7 cut(s) 20, 643, 766, 787, 813, 882, 1173
AluI AGCT 7 cut(s) 20, 643, 766, 787, 813, 882, 1173
Alw21I GWGCWC 2 cut(s) 207, 884
Alw26I GTCTC 3 cut(s) 46, 116, 157
AlwI GGATC 1 cut(s) 428
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 2 cut(s) 175, 620
ApeKI GCWGC 5 cut(s) 68, 87, 643, 1223, 1252
ApoI RAATTY 1 cut(s) 401
Asp700I GAANNNNTTC 2 cut(s) 1028, 1118
AspLEI GCGC 2 cut(s) 429, 776
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 934, 1237
AsuII TTCGAA 1 cut(s) 397
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 161
BanII GRGCYC 3 cut(s) 227, 249, 884
Bbv12I GWGCWC 2 cut(s) 207, 884
BbvCI CCTCAGC 1 cut(s) 59
BbvI GCAGC 5 cut(s) 55, 74, 630, 1210, 1239
BccI CCATC 3 cut(s) 164, 355, 365
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 2 cut(s) 221, 717
BcoDI GTCTC 3 cut(s) 46, 116, 157
BfaI CTAG 2 cut(s) 17, 971
BfmI CTRYAG 1 cut(s) 1253
BisI GCNGC 6 cut(s) 69, 88, 175, 644, 1224, 1253
BlsI GCNGC 6 cut(s) 70, 89, 176, 645, 1225, 1254
Bme1390I CCNGG 2 cut(s) 221, 717
Bme18I GGWCC 1 cut(s) 161
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 2 cut(s) 221, 717
BmsI GCATC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 1 cut(s) 248
Bpu10I CCTNAGC 1 cut(s) 59
Bpu14I TTCGAA 1 cut(s) 397
BpuEI CTTGAG 2 cut(s) 512, 539
BsaBI GATNNNNATC 1 cut(s) 1131
BsaJI CCNNGG 2 cut(s) 220, 1199
BsaWI WCCGGW 1 cut(s) 681
BsaXI ACNNNNNCTCC 4 cut(s) 137, 167, 194, 224
Bse118I RCCGGY 1 cut(s) 71
Bse3DI GCAATG 3 cut(s) 70, 633, 920
Bse8I GATNNNNATC 1 cut(s) 1131
BseBI CCWGG 2 cut(s) 221, 717
BseDI CCNNGG 2 cut(s) 220, 1199
BseGI GGATG 2 cut(s) 994, 1084
BseJI GATNNNNATC 1 cut(s) 1131
BseMI GCAATG 3 cut(s) 70, 633, 920
BseMII CTCAG 3 cut(s) 73, 202, 349
BseRI GAGGAG 4 cut(s) 47, 104, 215, 1058
BseXI GCAGC 5 cut(s) 55, 74, 630, 1210, 1239
Bsh1236I CGCG 2 cut(s) 429, 774
BshFI GGCC 2 cut(s) 177, 622
BsiHKAI GWGCWC 2 cut(s) 207, 884
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 2 cut(s) 72, 682
BslFI GGGAC 2 cut(s) 86, 147
BsmAI GTCTC 3 cut(s) 46, 116, 157
BsmBI CGTCTC 1 cut(s) 157
BsmFI GGGAC 2 cut(s) 86, 147
BsnI GGCC 2 cut(s) 177, 622
BsoBI CYCGRG 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 397
Bsp1286I GDGCHC 4 cut(s) 207, 227, 249, 884
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 7 cut(s) 10, 36, 195, 433, 532, 553, 822
Bsp19I CCATGG 1 cut(s) 1199
BspACI CCGC 3 cut(s) 104, 174, 945
BspANI GGCC 2 cut(s) 177, 622
BspCNI CTCAG 3 cut(s) 72, 201, 348
BspFNI CGCG 2 cut(s) 429, 774
BspHI TCATGA 1 cut(s) 876
BspLI GGNNCC 1 cut(s) 163
BspMAI CTGCAG 1 cut(s) 1257
BspPI GGATC 1 cut(s) 428
BspT104I TTCGAA 1 cut(s) 397
BsrDI GCAATG 3 cut(s) 70, 633, 920
BsrFI RCCGGY 1 cut(s) 71
BsrGI TGTACA 1 cut(s) 27
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 2 cut(s) 220, 1199
BssMI GATC 7 cut(s) 10, 36, 195, 433, 532, 553, 822
BssT1I CCWWGG 1 cut(s) 1199
Bst2UI CCWGG 2 cut(s) 221, 717
Bst4CI ACNGT 3 cut(s) 410, 899, 1270
BstAUI TGTACA 1 cut(s) 27
BstBI TTCGAA 1 cut(s) 397
BstC8I GCNNGC 5 cut(s) 73, 179, 588, 662, 1171
BstDEI CTNAG 3 cut(s) 59, 188, 335
BstDSI CCRYGG 1 cut(s) 1199
BstF5I GGATG 2 cut(s) 994, 1084
BstFNI CGCG 2 cut(s) 429, 774
BstHHI GCGC 2 cut(s) 429, 776
BstKTI GATC 7 cut(s) 13, 39, 198, 436, 535, 556, 825
BstMAI GTCTC 3 cut(s) 46, 116, 157
BstMBI GATC 7 cut(s) 10, 36, 195, 433, 532, 553, 822
BstMWI GCNNNNNNNGC 2 cut(s) 211, 1223
BstNI CCWGG 2 cut(s) 221, 717
BstNSI RCATGY 2 cut(s) 664, 1160
BstSCI CCNGG 2 cut(s) 219, 715
BstSFI CTRYAG 1 cut(s) 1253
BstUI CGCG 2 cut(s) 429, 774
BstV1I GCAGC 5 cut(s) 55, 74, 630, 1210, 1239
BsuRI GGCC 2 cut(s) 177, 622
BtgI CCRYGG 1 cut(s) 1199
BtgZI GCGATG 1 cut(s) 444
BtsCI GGATG 2 cut(s) 994, 1084
BtsIMutI CAGTG 1 cut(s) 1266
Cac8I GCNNGC 5 cut(s) 73, 179, 588, 662, 1171
CciI TCATGA 1 cut(s) 876
CfoI GCGC 2 cut(s) 429, 776
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 161
Csp6I GTAC 4 cut(s) 28, 136, 732, 1180
CviAII CATG 7 cut(s) 25, 661, 707, 877, 927, 1157, 1200
CviQI GTAC 4 cut(s) 28, 136, 732, 1180
DdeI CTNAG 3 cut(s) 59, 188, 335
DpnI GATC 7 cut(s) 12, 38, 197, 435, 534, 555, 824
DpnII GATC 7 cut(s) 10, 36, 195, 433, 532, 553, 822
DraI TTTAAA 1 cut(s) 594
EaeI YGGCCR 1 cut(s) 175
Ecl136II GAGCTC 1 cut(s) 882
Eco130I CCWWGG 1 cut(s) 1199
Eco24I GRGCYC 3 cut(s) 227, 249, 884
Eco47I GGWCC 1 cut(s) 161
Eco53kI GAGCTC 1 cut(s) 882
Eco88I CYCGRG 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 882
EcoRII CCWGG 2 cut(s) 219, 715
EcoT14I CCWWGG 1 cut(s) 1199
EcoT22I ATGCAT 1 cut(s) 666
EcoT38I GRGCYC 3 cut(s) 227, 249, 884
ErhI CCWWGG 1 cut(s) 1199
Esp3I CGTCTC 1 cut(s) 157
FaeI CATG 7 cut(s) 28, 664, 710, 880, 930, 1160, 1203
FaqI GGGAC 2 cut(s) 86, 147
FatI CATG 7 cut(s) 24, 660, 706, 876, 926, 1156, 1199
FauI CCCGC 1 cut(s) 111
FauNDI CATATG 1 cut(s) 612
Fnu4HI GCNGC 6 cut(s) 69, 88, 175, 644, 1224, 1253
FokI GGATG 2 cut(s) 1001, 1091
FriOI GRGCYC 3 cut(s) 227, 249, 884
Fsp4HI GCNGC 6 cut(s) 69, 88, 175, 644, 1224, 1253
FspBI CTAG 2 cut(s) 17, 971
GlaI GCGC 2 cut(s) 428, 775
GluI GCNGC 6 cut(s) 69, 88, 175, 644, 1224, 1253
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 2 cut(s) 177, 622
HapII CCGG 2 cut(s) 72, 682
HhaI GCGC 2 cut(s) 429, 776
Hin1II CATG 7 cut(s) 28, 664, 710, 880, 930, 1160, 1203
Hin6I GCGC 2 cut(s) 427, 774
HinP1I GCGC 2 cut(s) 427, 774
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HindIII AAGCTT 3 cut(s) 764, 811, 1171
HinfI GANTC 8 cut(s) 54, 80, 99, 109, 259, 303, 563, 780
HpaII CCGG 2 cut(s) 72, 682
HphI GGTGA 2 cut(s) 934, 1237
Hpy166II GTNNAC 2 cut(s) 258, 1182
Hpy188I TCNGA 8 cut(s) 51, 98, 191, 308, 322, 537, 550, 1034
Hpy188III TCNNGA 7 cut(s) 118, 237, 491, 560, 877, 1021, 1290
Hpy8I GTNNAC 2 cut(s) 258, 1182
Hpy99I CGWCG 1 cut(s) 774
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 3 cut(s) 410, 899, 1270
HpyCH4IV ACGT 1 cut(s) 956
HpyCH4V TGCA 5 cut(s) 90, 638, 646, 664, 1255
HpyF10VI GCNNNNNNNGC 2 cut(s) 211, 1223
HpyF3I CTNAG 3 cut(s) 59, 188, 335
HpySE526I ACGT 1 cut(s) 956
Hsp92II CATG 7 cut(s) 28, 664, 710, 880, 930, 1160, 1203
HspAI GCGC 2 cut(s) 427, 774
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Kzo9I GATC 7 cut(s) 10, 36, 195, 433, 532, 553, 822
LmnI GCTCC 2 cut(s) 202, 935
Lsp1109I GCAGC 5 cut(s) 55, 74, 630, 1210, 1239
LweI GCATC 1 cut(s) 99
MaeI CTAG 2 cut(s) 17, 971
MaeII ACGT 1 cut(s) 956
MaeIII GTNAC 2 cut(s) 215, 314
MalI GATC 7 cut(s) 12, 38, 197, 435, 534, 555, 824
MboI GATC 7 cut(s) 10, 36, 195, 433, 532, 553, 822
MboII GAAGA 6 cut(s) 46, 85, 308, 476, 563, 592
MhlI GDGCHC 4 cut(s) 207, 227, 249, 884
MluCI AATT 5 cut(s) 401, 459, 1024, 1066, 1115
MlyI GAGTC 7 cut(s) 48, 74, 108, 118, 253, 312, 557
MmeI TCCRAC 1 cut(s) 528
MnlI CCTC 9 cut(s) 57, 68, 125, 193, 197, 344, 804, 1036, 1039
Mph1103I ATGCAT 1 cut(s) 666
MroNI GCCGGC 1 cut(s) 71
MroXI GAANNNNTTC 2 cut(s) 1028, 1118
MseI TTAA 5 cut(s) 123, 383, 593, 1062, 1274
MslI CAYNNNNRTG 3 cut(s) 356, 446, 528
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 2 cut(s) 72, 682
MspR9I CCNGG 2 cut(s) 221, 717
MvaI CCWGG 2 cut(s) 221, 717
MvnI CGCG 2 cut(s) 429, 774
MwoI GCNNNNNNNGC 2 cut(s) 211, 1223
NaeI GCCGGC 1 cut(s) 73
NcoI CCATGG 1 cut(s) 1199
NdeI CATATG 1 cut(s) 612
NdeII GATC 7 cut(s) 10, 36, 195, 433, 532, 553, 822
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 7 cut(s) 28, 664, 710, 880, 930, 1160, 1203
NlaIV GGNNCC 1 cut(s) 163
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 2 cut(s) 664, 1160
NspV TTCGAA 1 cut(s) 397
PaeI GCATGC 1 cut(s) 664
PaeR7I CTCGAG 1 cut(s) 116
PagI TCATGA 1 cut(s) 876
PdiI GCCGGC 1 cut(s) 73
PdmI GAANNNNTTC 2 cut(s) 1028, 1118
PfeI GAWTC 1 cut(s) 780
PkrI GCNGC 6 cut(s) 70, 89, 176, 645, 1225, 1254
PleI GAGTC 7 cut(s) 48, 74, 107, 117, 253, 311, 557
PpsI GAGTC 7 cut(s) 48, 74, 107, 117, 253, 311, 557
Psp124BI GAGCTC 1 cut(s) 884
Psp1406I AACGTT 1 cut(s) 956
Psp6I CCWGG 2 cut(s) 219, 715
PspGI CCWGG 2 cut(s) 219, 715
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 1 cut(s) 161
PstI CTGCAG 1 cut(s) 1257
RsaI GTAC 4 cut(s) 29, 137, 733, 1181
RsaNI GTAC 4 cut(s) 28, 136, 732, 1180
RseI CAYNNNNRTG 3 cut(s) 356, 446, 528
SacI GAGCTC 1 cut(s) 884
SaqAI TTAA 5 cut(s) 123, 383, 593, 1062, 1274
SatI GCNGC 6 cut(s) 69, 88, 175, 644, 1224, 1253
Sau3AI GATC 7 cut(s) 10, 36, 195, 433, 532, 553, 822
Sau96I GGNCC 1 cut(s) 161
SchI GAGTC 7 cut(s) 48, 74, 108, 118, 253, 312, 557
ScrFI CCNGG 2 cut(s) 221, 717
SduI GDGCHC 4 cut(s) 207, 227, 249, 884
SfaNI GCATC 1 cut(s) 99
SfcI CTRYAG 1 cut(s) 1253
Sfr274I CTCGAG 1 cut(s) 116
SfuI TTCGAA 1 cut(s) 397
SinI GGWCC 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 116
SmiMI CAYNNNNRTG 3 cut(s) 356, 446, 528
SmlI CTYRAG 3 cut(s) 116, 491, 518
SmoI CTYRAG 3 cut(s) 116, 491, 518
SphI GCATGC 1 cut(s) 664
Sse9I AATT 5 cut(s) 401, 459, 1024, 1066, 1115
SsiI CCGC 3 cut(s) 104, 174, 945
SspMI CTAG 2 cut(s) 17, 971
SstI GAGCTC 1 cut(s) 884
StyD4I CCNGG 2 cut(s) 219, 715
StyI CCWWGG 1 cut(s) 1199
TaaI ACNGT 3 cut(s) 410, 899, 1270
TaiI ACGT 1 cut(s) 959
TaqI TCGA 9 cut(s) 117, 198, 354, 387, 397, 846, 993, 1020, 1112
TaqII GACCGA 1 cut(s) 149
TasI AATT 5 cut(s) 401, 459, 1024, 1066, 1115
TatI WGTACW 2 cut(s) 27, 1179
TauI GCSGC 1 cut(s) 177
TfiI GAWTC 1 cut(s) 780
Tru1I TTAA 5 cut(s) 123, 383, 593, 1062, 1274
Tru9I TTAA 5 cut(s) 123, 383, 593, 1062, 1274
TscAI CASTG 1 cut(s) 1273
TseI GCWGC 5 cut(s) 68, 87, 643, 1223, 1252
TspDTI ATGAA 5 cut(s) 17, 340, 865, 1110, 1121
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 1 cut(s) 1273
VpaK11BI GGWCC 1 cut(s) 161
XapI RAATTY 1 cut(s) 401
XceI RCATGY 2 cut(s) 664, 1160
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
XhoI CTCGAG 1 cut(s) 116
XmnI GAANNNNTTC 2 cut(s) 1028, 1118
XspI CTAG 2 cut(s) 17, 971
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.