Rw3G016490

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002063
Physical Location & Seq
Forward (+)
17500341 .. 17501824
1484 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G016490.1

Sequence Viewer

Length: 1293 bp
ATGCAGACCAGTCCAGTTCAAGAAGAACACAACCATGTCACCCCCATCTCCGAAATGAACAATGATCGCCTAGCTTCCATGTACAAGAAGATCGCAGACCCTCCGAGACTCCTCAGCAATGCCGCCGGCAAAGACTCCTGCTGCATCTTCCGAGTCCCGCAGAGTCTCCTCGAGATTAACGGCAAGTCGTACCAGCCCCACATCGTCTCCATCGGTCCCTTCCACCGCGGCCAGCCCCACCTCAGAATGATCGAGGAGCACAAGTGGCGTTACCTGGGCTCTCTGCTTTCAAGAACAGAGCCCAAAGGGTTGACTCTCCAGCACTACTTGAAGTCCATAGAGCCAATGGAAGATAGAGTCAGAGAGTGTTACTCTGAAACAATCCACCTCAGTAGTGATGAGTTCGTCGAAATGATGGTTGTTGATGGTTGCTTCTTAATCGAGTTGTTTCGAAAATTTGACCGTGTGGTTCGGTTTGAGCGCGATGATCCACTCGTCAATATGTTCTGGGTAATGCCGTTTATGGTTAGAGATTTTCTTCTCGGAAGATCATATCGTCAAGACTCTGTGAAGTCTTTGTCCTTGCTTGCTTTAAACTTCTTCAACAACCATATGGACAGGCCGAAAGAAGTCATTGCAAAGCTGCATAATCTTACAGCTGAGCATTTACTTGACTTACTCCGGTCAAGTGTGATACCAGCCGACCATGTTGAACCAGGACACAAGAATAGTACCCCAACCCACATTATACATTGTATTTCAAAGCTTCGTCGCGCTGGAATCAAGCTCAATCCTCCTAAAGAATACGAAAGCTTCTTGGCGATCAAGTTCAAGCGTGGAGTTATCGAAATGCCCCCAATCATCATAGACGATTTCATGAGCTCGTTCTTGTTGAACTGTGTAGCCTATGAGCAATGCCACAAGTCATGCTCCAAACACATCACCGCTTACGCAACGTTGCTGGATTACCTAGTGAATACTTACAAGGATGTCGAGTATTTGGCTGACAGAAACATAATCGAGAATTGCTTCGGAAACGATGAGGAGGTTGCTCATTTTATTAACAATTTGGGTAAGGATGTGGCTTTTGATGGTGAAAGATGTTATCTGTCGAAATTGTTCAATGATGTTCATCAGTATTACAGCAATAGTAGGCATGTTCAATGGGCAAGCTTCAAGTACACTTACTTTGACACTCCATGGTCGTTTATTTCGGCTTTTGCTGCGTTGATTTTGCTCATATTCACCTTGCTGCAGACCTTATACACTGTTTTAAGTTATTATCATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

430

Amino Acids

50.06

Weight (kDa)

6.6

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 49 - 415 1.4e-107 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 228, 483, 774
AciI CCGC 5 cut(s) 123, 158, 226, 228, 945
AclI AACGTT 1 cut(s) 956
AclWI GGATC 1 cut(s) 482
AcoI YGGCCR 1 cut(s) 229
AcsI RAATTY 1 cut(s) 455
AfaI GTAC 4 cut(s) 83, 191, 733, 1181
AjnI CCWGG 2 cut(s) 273, 715
AluBI AGCT 8 cut(s) 74, 643, 659, 766, 787, 813, 882, 1173
AluI AGCT 8 cut(s) 74, 643, 659, 766, 787, 813, 882, 1173
Alw21I GWGCWC 2 cut(s) 261, 884
Alw26I GTCTC 3 cut(s) 100, 170, 211
AlwI GGATC 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 2 cut(s) 229, 620
ApeKI GCWGC 4 cut(s) 141, 643, 1223, 1252
ApoI RAATTY 1 cut(s) 455
Asp700I GAANNNNTTC 2 cut(s) 1028, 1118
AspLEI GCGC 2 cut(s) 483, 776
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 4 cut(s) 31, 934, 1106, 1237
AsuII TTCGAA 1 cut(s) 451
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 1 cut(s) 215
BanII GRGCYC 3 cut(s) 281, 303, 884
Bbv12I GWGCWC 2 cut(s) 261, 884
BbvCI CCTCAGC 1 cut(s) 113
BbvI GCAGC 4 cut(s) 128, 630, 1210, 1239
BccI CCATC 5 cut(s) 53, 218, 409, 419, 1085
BceAI ACGGC 2 cut(s) 196, 502
BciT130I CCWGG 2 cut(s) 275, 717
BcoDI GTCTC 3 cut(s) 100, 170, 211
BfaI CTAG 2 cut(s) 71, 971
BfmI CTRYAG 1 cut(s) 1253
BisI GCNGC 6 cut(s) 123, 142, 229, 644, 1224, 1253
BlpI GCTNAGC 1 cut(s) 660
BlsI GCNGC 6 cut(s) 124, 143, 230, 645, 1225, 1254
Bme1390I CCNGG 2 cut(s) 275, 717
Bme18I GGWCC 1 cut(s) 215
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 2 cut(s) 275, 717
BmsI GCATC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpmI CTGGAG 1 cut(s) 302
Bpu10I CCTNAGC 1 cut(s) 113
Bpu1102I GCTNAGC 1 cut(s) 660
Bpu14I TTCGAA 1 cut(s) 451
BsaBI GATNNNNATC 1 cut(s) 1131
BsaJI CCNNGG 3 cut(s) 226, 274, 1199
BsaWI WCCGGW 1 cut(s) 681
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 248, 278
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 9, 14
Bse3DI GCAATG 3 cut(s) 124, 633, 920
Bse8I GATNNNNATC 1 cut(s) 1131
BseBI CCWGG 2 cut(s) 275, 717
BseDI CCNNGG 3 cut(s) 226, 274, 1199
BseGI GGATG 2 cut(s) 994, 1084
BseJI GATNNNNATC 1 cut(s) 1131
BseMI GCAATG 3 cut(s) 124, 633, 920
BseMII CTCAG 4 cut(s) 127, 256, 403, 651
BseNI ACTGG 2 cut(s) 9, 14
BseRI GAGGAG 4 cut(s) 101, 158, 269, 1058
BseXI GCAGC 4 cut(s) 128, 630, 1210, 1239
Bsh1236I CGCG 3 cut(s) 228, 483, 774
BshFI GGCC 2 cut(s) 231, 622
BsiHKAI GWGCWC 2 cut(s) 261, 884
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 2 cut(s) 126, 682
BslFI GGGAC 2 cut(s) 140, 201
BsmAI GTCTC 3 cut(s) 100, 170, 211
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 2 cut(s) 140, 201
BsnI GGCC 2 cut(s) 231, 622
BsoBI CYCGRG 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 451
Bsp1286I GDGCHC 4 cut(s) 261, 281, 303, 884
Bsp1407I TGTACA 1 cut(s) 81
Bsp143I GATC 6 cut(s) 64, 90, 249, 487, 548, 822
Bsp1720I GCTNAGC 1 cut(s) 660
Bsp19I CCATGG 1 cut(s) 1199
BspACI CCGC 5 cut(s) 123, 158, 226, 228, 945
BspANI GGCC 2 cut(s) 231, 622
BspCNI CTCAG 4 cut(s) 126, 255, 402, 652
BspFNI CGCG 3 cut(s) 228, 483, 774
BspHI TCATGA 1 cut(s) 876
BspLI GGNNCC 1 cut(s) 217
BspMAI CTGCAG 1 cut(s) 1257
BspPI GGATC 1 cut(s) 482
BspT104I TTCGAA 1 cut(s) 451
BsrDI GCAATG 3 cut(s) 124, 633, 920
BsrFI RCCGGY 1 cut(s) 125
BsrGI TGTACA 1 cut(s) 81
BsrI ACTGG 2 cut(s) 9, 14
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 3 cut(s) 226, 274, 1199
BssMI GATC 6 cut(s) 64, 90, 249, 487, 548, 822
BssT1I CCWWGG 1 cut(s) 1199
Bst2UI CCWGG 2 cut(s) 275, 717
Bst4CI ACNGT 3 cut(s) 464, 899, 1270
BstAUI TGTACA 1 cut(s) 81
BstBI TTCGAA 1 cut(s) 451
BstC8I GCNNGC 4 cut(s) 127, 233, 588, 1171
BstDEI CTNAG 4 cut(s) 113, 242, 389, 660
BstDSI CCRYGG 2 cut(s) 226, 1199
BstF5I GGATG 2 cut(s) 994, 1084
BstFNI CGCG 3 cut(s) 228, 483, 774
BstHHI GCGC 2 cut(s) 483, 776
BstKTI GATC 6 cut(s) 67, 93, 252, 490, 551, 825
BstMAI GTCTC 3 cut(s) 100, 170, 211
BstMBI GATC 6 cut(s) 64, 90, 249, 487, 548, 822
BstMWI GCNNNNNNNGC 2 cut(s) 265, 1223
BstNI CCWGG 2 cut(s) 275, 717
BstNSI RCATGY 1 cut(s) 1160
BstSCI CCNGG 2 cut(s) 273, 715
BstSFI CTRYAG 1 cut(s) 1253
BstUI CGCG 3 cut(s) 228, 483, 774
BstV1I GCAGC 4 cut(s) 128, 630, 1210, 1239
BsuRI GGCC 2 cut(s) 231, 622
BtgI CCRYGG 2 cut(s) 226, 1199
BtgZI GCGATG 1 cut(s) 498
BtsCI GGATG 2 cut(s) 994, 1084
BtsIMutI CAGTG 1 cut(s) 1266
Cac8I GCNNGC 4 cut(s) 127, 233, 588, 1171
CciI TCATGA 1 cut(s) 876
CfoI GCGC 2 cut(s) 483, 776
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 215
Cfr42I CCGCGG 1 cut(s) 229
Csp6I GTAC 4 cut(s) 82, 190, 732, 1180
CviAII CATG 7 cut(s) 35, 79, 707, 877, 927, 1157, 1200
CviQI GTAC 4 cut(s) 82, 190, 732, 1180
DdeI CTNAG 4 cut(s) 113, 242, 389, 660
DpnI GATC 6 cut(s) 66, 92, 251, 489, 550, 824
DpnII GATC 6 cut(s) 64, 90, 249, 487, 548, 822
DraI TTTAAA 1 cut(s) 594
EaeI YGGCCR 1 cut(s) 229
Ecl136II GAGCTC 1 cut(s) 882
Eco130I CCWWGG 1 cut(s) 1199
Eco24I GRGCYC 3 cut(s) 281, 303, 884
Eco47I GGWCC 1 cut(s) 215
Eco53kI GAGCTC 1 cut(s) 882
Eco88I CYCGRG 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 882
EcoRII CCWGG 2 cut(s) 273, 715
EcoT14I CCWWGG 1 cut(s) 1199
EcoT38I GRGCYC 3 cut(s) 281, 303, 884
ErhI CCWWGG 1 cut(s) 1199
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 7 cut(s) 38, 82, 710, 880, 930, 1160, 1203
FaqI GGGAC 2 cut(s) 140, 201
FatI CATG 7 cut(s) 34, 78, 706, 876, 926, 1156, 1199
FauI CCCGC 1 cut(s) 165
FauNDI CATATG 1 cut(s) 612
Fnu4HI GCNGC 6 cut(s) 123, 142, 229, 644, 1224, 1253
FokI GGATG 2 cut(s) 1001, 1091
FriOI GRGCYC 3 cut(s) 281, 303, 884
Fsp4HI GCNGC 6 cut(s) 123, 142, 229, 644, 1224, 1253
FspBI CTAG 2 cut(s) 71, 971
GlaI GCGC 2 cut(s) 482, 775
GluI GCNGC 6 cut(s) 123, 142, 229, 644, 1224, 1253
GsuI CTGGAG 1 cut(s) 302
HaeIII GGCC 2 cut(s) 231, 622
HapII CCGG 2 cut(s) 126, 682
HhaI GCGC 2 cut(s) 483, 776
Hin1II CATG 7 cut(s) 38, 82, 710, 880, 930, 1160, 1203
Hin6I GCGC 2 cut(s) 481, 774
HinP1I GCGC 2 cut(s) 481, 774
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HindIII AAGCTT 3 cut(s) 764, 811, 1171
HinfI GANTC 8 cut(s) 108, 134, 153, 163, 313, 357, 563, 780
HpaII CCGG 2 cut(s) 126, 682
HphI GGTGA 4 cut(s) 31, 934, 1106, 1237
Hpy166II GTNNAC 2 cut(s) 312, 1182
Hpy188I TCNGA 8 cut(s) 52, 105, 152, 245, 362, 376, 545, 1034
Hpy188III TCNNGA 7 cut(s) 20, 172, 291, 560, 877, 1021, 1290
Hpy8I GTNNAC 2 cut(s) 312, 1182
Hpy99I CGWCG 2 cut(s) 410, 774
HpyAV CCTTC 1 cut(s) 229
HpyCH4III ACNGT 3 cut(s) 464, 899, 1270
HpyCH4IV ACGT 1 cut(s) 956
HpyCH4V TGCA 5 cut(s) 4, 144, 638, 646, 1255
HpyF10VI GCNNNNNNNGC 2 cut(s) 265, 1223
HpyF3I CTNAG 4 cut(s) 113, 242, 389, 660
HpySE526I ACGT 1 cut(s) 956
Hsp92II CATG 7 cut(s) 38, 82, 710, 880, 930, 1160, 1203
HspAI GCGC 2 cut(s) 481, 774
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 6 cut(s) 64, 90, 249, 487, 548, 822
LmnI GCTCC 2 cut(s) 256, 935
Lsp1109I GCAGC 4 cut(s) 128, 630, 1210, 1239
LweI GCATC 1 cut(s) 153
MaeI CTAG 2 cut(s) 71, 971
MaeII ACGT 1 cut(s) 956
MaeIII GTNAC 3 cut(s) 37, 269, 368
MalI GATC 6 cut(s) 66, 92, 251, 489, 550, 824
MboI GATC 6 cut(s) 64, 90, 249, 487, 548, 822
MboII GAAGA 7 cut(s) 35, 100, 139, 362, 530, 558, 592
MhlI GDGCHC 4 cut(s) 261, 281, 303, 884
MluCI AATT 4 cut(s) 455, 1024, 1066, 1115
MlyI GAGTC 7 cut(s) 102, 128, 162, 172, 307, 366, 557
MnlI CCTC 9 cut(s) 111, 122, 179, 247, 251, 398, 804, 1036, 1039
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 2 cut(s) 1028, 1118
MseI TTAA 5 cut(s) 177, 437, 593, 1062, 1274
MslI CAYNNNNRTG 1 cut(s) 33
MspA1I CMGCKG 2 cut(s) 228, 659
MspI CCGG 2 cut(s) 126, 682
MspR9I CCNGG 2 cut(s) 275, 717
MvaI CCWGG 2 cut(s) 275, 717
MvnI CGCG 3 cut(s) 228, 483, 774
MwoI GCNNNNNNNGC 2 cut(s) 265, 1223
NaeI GCCGGC 1 cut(s) 127
NcoI CCATGG 1 cut(s) 1199
NdeI CATATG 1 cut(s) 612
NdeII GATC 6 cut(s) 64, 90, 249, 487, 548, 822
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 7 cut(s) 38, 82, 710, 880, 930, 1160, 1203
NlaIV GGNNCC 1 cut(s) 217
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 1 cut(s) 1160
NspV TTCGAA 1 cut(s) 451
PaeR7I CTCGAG 1 cut(s) 170
PagI TCATGA 1 cut(s) 876
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 2 cut(s) 1028, 1118
PfeI GAWTC 1 cut(s) 780
PkrI GCNGC 6 cut(s) 124, 143, 230, 645, 1225, 1254
PleI GAGTC 7 cut(s) 102, 128, 161, 171, 307, 365, 557
PpsI GAGTC 7 cut(s) 102, 128, 161, 171, 307, 365, 557
Psp124BI GAGCTC 1 cut(s) 884
Psp1406I AACGTT 1 cut(s) 956
Psp6I CCWGG 2 cut(s) 273, 715
PspGI CCWGG 2 cut(s) 273, 715
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 215
PstI CTGCAG 1 cut(s) 1257
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 4 cut(s) 83, 191, 733, 1181
RsaNI GTAC 4 cut(s) 82, 190, 732, 1180
RseI CAYNNNNRTG 1 cut(s) 33
SacI GAGCTC 1 cut(s) 884
SacII CCGCGG 1 cut(s) 229
SaqAI TTAA 5 cut(s) 177, 437, 593, 1062, 1274
SatI GCNGC 6 cut(s) 123, 142, 229, 644, 1224, 1253
Sau3AI GATC 6 cut(s) 64, 90, 249, 487, 548, 822
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 7 cut(s) 102, 128, 162, 172, 307, 366, 557
ScrFI CCNGG 2 cut(s) 275, 717
SduI GDGCHC 4 cut(s) 261, 281, 303, 884
SfaNI GCATC 1 cut(s) 153
SfcI CTRYAG 1 cut(s) 1253
Sfr274I CTCGAG 1 cut(s) 170
Sfr303I CCGCGG 1 cut(s) 229
SfuI TTCGAA 1 cut(s) 451
SgrBI CCGCGG 1 cut(s) 229
SinI GGWCC 1 cut(s) 215
SlaI CTCGAG 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 4 cut(s) 455, 1024, 1066, 1115
SsiI CCGC 5 cut(s) 123, 158, 226, 228, 945
SspMI CTAG 2 cut(s) 71, 971
SstI GAGCTC 1 cut(s) 884
StyD4I CCNGG 2 cut(s) 273, 715
StyI CCWWGG 1 cut(s) 1199
TaaI ACNGT 3 cut(s) 464, 899, 1270
TaiI ACGT 1 cut(s) 959
TaqI TCGA 9 cut(s) 171, 252, 408, 441, 451, 846, 993, 1020, 1112
TaqII GACCGA 1 cut(s) 203
TasI AATT 4 cut(s) 455, 1024, 1066, 1115
TatI WGTACW 2 cut(s) 81, 1179
TauI GCSGC 2 cut(s) 125, 231
TfiI GAWTC 1 cut(s) 780
Tru1I TTAA 5 cut(s) 177, 437, 593, 1062, 1274
Tru9I TTAA 5 cut(s) 177, 437, 593, 1062, 1274
TscAI CASTG 1 cut(s) 1273
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 4 cut(s) 141, 643, 1223, 1252
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 3 cut(s) 71, 865, 1121
TspRI CASTG 1 cut(s) 1273
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 1 cut(s) 455
XceI RCATGY 1 cut(s) 1160
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 170
XmnI GAANNNNTTC 2 cut(s) 1028, 1118
XspI CTAG 2 cut(s) 71, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.