Rroxscaffold_6G00409070

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002063
Physical Location & Seq
Forward (+)
31591206 .. 31592830
1625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00409070.1

Sequence Viewer

Length: 1194 bp
ATGGATACCAATTCAGTTCAAGAAGAACACAACCATGTCACCCCCACCTGGGAAATGAACAATGATCGCTTAGCTTCCATGTACCAGAAGATCGCAGACCCTCCACGACTCCTCAGCAACGCTGCCGGCAAAGCCTCCTGTTGCATCTTCCGAGTCCCGCAGAGCCTCCTCGAGATAAACGGCAAGTCTTACCAGCCCCACATCGTCTCCATCGGCCCCTACCACCGCGGGAAGCCCCACCTCAGAATGATCGAGGAGCACAAGTGGCGCTACCTGGGCTCTCTGCTTTCAAGAACAGAGCCCAAAGGGTTGACTCTCCAGCACTACTTGAAGTCCATAGAGCCAATGGAAGCTAGAGCCAGGGAGTGTTACTCTGAGACCATCCATCTCAGCACTGATGAGTTCCTAGAAATGATGGTTGTTGATGGTTGCTTCTTGATTGAGTTGTTTCGAAAAGTTGGCCATGTGGTTCGGTTTGAGCATGATGATCCACTCATTAATATGTTTTGGATACTACCGTTTATGGTCAGGGATTTTCTTCGACTTGAGAACCAAATGCCATACTTTGTTCTTGAGCACTTGTATGATCTGATAACCAAGGTCGAAGATCGTAAAGACTCTGTGAAATCCTTGTCCTTGCTTGCTTTGGAATTCTTCAACAACCATATGATGAGGCCTGACCCTGTCATAGAAAAGCTCCATAGTCTTACAGGTATGCATTTGCTTGACTTGTTGCGGTCAAGTGTGATACCAGCGGGTTATGCAGAGCCACAAAGAAGAAACAGTACACCAACGCACATAATACATTGTGTGTCTAAGCTTCGTCGCGCTGGTATCAAGCTCAATGTTAAGGAATTCGAAAGCTTCTTGGTGGTCAAATTCAAGCGTGGAGCGATCGAGATGCCATCCATCACAATCGACGATTTCATGTGCGCTTTCTTGTTGAACTGCGTGGCCTATGAGCAGTGCCACAAGTCATGCTCGAAACACATCACCACGTATGCCACATTCCTCGATTGCCTCGTGAACAGTTACAAGGATGTCGAGTATTTGTGTGACAGAAACATAATGGAGAATTGCTATGGGAACGATGGGGAAATTGCTCGTTTCATTAACAATATGGGGAAGGATGTGGCTTTTGATTATGATAGGTGTTATCTGTCGAAATTGTTCAATGATGTTCATCAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

397

Amino Acids

46.29

Weight (kDa)

6.44

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 49 - 397 8.4e-108 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 228, 828
AciI CCGC 5 cut(s) 158, 226, 228, 736, 755
AclWI GGATC 1 cut(s) 482
AcoI YGGCCR 1 cut(s) 460
AcsI RAATTY 3 cut(s) 650, 854, 878
AfaI GTAC 2 cut(s) 83, 787
AfiI CCNNNNNNNGG 2 cut(s) 48, 49
AgsI TTSAA 7 cut(s) 20, 291, 331, 658, 883, 946, 1174
AjnI CCWGG 3 cut(s) 47, 273, 359
AluBI AGCT 6 cut(s) 74, 353, 697, 820, 841, 864
AluI AGCT 6 cut(s) 74, 353, 697, 820, 841, 864
Alw21I GWGCWC 2 cut(s) 261, 579
Alw26I GTCTC 2 cut(s) 211, 371
AlwI GGATC 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 4 cut(s) 214, 460, 674, 954
ApeKI GCWGC 1 cut(s) 122
ApoI RAATTY 3 cut(s) 650, 854, 878
AseI ATTAAT 1 cut(s) 498
Asp700I GAANNNNTTC 1 cut(s) 1169
AspLEI GCGC 3 cut(s) 270, 830, 935
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 31, 985
AsuII TTCGAA 2 cut(s) 451, 858
AvaI CYCGRG 1 cut(s) 170
BaeI ACNNNNGTAYC 2 cut(s) 503, 536
BalI TGGCCA 1 cut(s) 462
BanII GRGCYC 2 cut(s) 281, 303
BarI GAAGNNNNNNTAC 2 cut(s) 769, 801
BauI CACGAG 1 cut(s) 1022
Bbv12I GWGCWC 2 cut(s) 261, 579
BbvCI CCTCAGC 1 cut(s) 113
BbvI GCAGC 1 cut(s) 109
BccI CCATC 8 cut(s) 218, 389, 393, 409, 419, 913, 917, 1085
BceAI ACGGC 1 cut(s) 196
BcgI CGANNNNNNTGC 2 cut(s) 883, 917
BciT130I CCWGG 3 cut(s) 49, 275, 361
BciVI GTATCC 1 cut(s) 504
BcoDI GTCTC 2 cut(s) 211, 371
BfaI CTAG 2 cut(s) 354, 407
BfoI RGCGCY 1 cut(s) 271
BfuI GTATCC 1 cut(s) 504
BisI GCNGC 1 cut(s) 123
BlpI GCTNAGC 1 cut(s) 70
BlsI GCNGC 1 cut(s) 124
Bme1390I CCNGG 3 cut(s) 49, 275, 361
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 3 cut(s) 49, 275, 361
BmsI GCATC 2 cut(s) 153, 891
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpmI CTGGAG 1 cut(s) 302
Bpu10I CCTNAGC 1 cut(s) 113
Bpu1102I GCTNAGC 1 cut(s) 70
Bpu14I TTCGAA 2 cut(s) 451, 858
BpuEI CTTGAG 2 cut(s) 566, 593
BsaAI YACGTR 1 cut(s) 999
BsaBI GATNNNNATC 1 cut(s) 1182
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 5 cut(s) 48, 226, 274, 360, 597
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 248, 278
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 49
Bse118I RCCGGY 1 cut(s) 125
Bse8I GATNNNNATC 1 cut(s) 1182
BseBI CCWGG 3 cut(s) 49, 275, 361
BseDI CCNNGG 5 cut(s) 48, 226, 274, 360, 597
BseGI GGATG 4 cut(s) 381, 905, 1045, 1135
BseJI GATNNNNATC 1 cut(s) 1182
BseLI CCNNNNNNNGG 2 cut(s) 48, 49
BseMII CTCAG 4 cut(s) 127, 256, 366, 403
BseRI GAGGAG 3 cut(s) 101, 158, 269
BseXI GCAGC 1 cut(s) 109
Bsh1236I CGCG 2 cut(s) 228, 828
Bsh1285I CGRYCG 1 cut(s) 897
BshFI GGCC 4 cut(s) 216, 462, 676, 956
BsiEI CGRYCG 1 cut(s) 897
BsiHKAI GWGCWC 2 cut(s) 261, 579
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 1 cut(s) 126
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 2 cut(s) 48, 49
BsmAI GTCTC 2 cut(s) 211, 371
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 4 cut(s) 216, 462, 676, 956
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 170
Bsp119I TTCGAA 2 cut(s) 451, 858
Bsp1286I GDGCHC 4 cut(s) 261, 281, 303, 579
Bsp143I GATC 7 cut(s) 64, 90, 249, 487, 586, 607, 894
Bsp1720I GCTNAGC 1 cut(s) 70
BspACI CCGC 5 cut(s) 158, 226, 228, 736, 755
BspANI GGCC 4 cut(s) 216, 462, 676, 956
BspCNI CTCAG 4 cut(s) 126, 255, 367, 402
BspFNI CGCG 2 cut(s) 228, 828
BspLI GGNNCC 1 cut(s) 217
BspPI GGATC 1 cut(s) 482
BspT104I TTCGAA 2 cut(s) 451, 858
BspTNI GGTCTC 1 cut(s) 371
BsrFI RCCGGY 1 cut(s) 125
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 5 cut(s) 48, 226, 274, 360, 597
BssMI GATC 7 cut(s) 64, 90, 249, 487, 586, 607, 894
BssSI CACGAG 1 cut(s) 1022
BssT1I CCWWGG 1 cut(s) 597
Bst2BI CACGAG 1 cut(s) 1022
Bst2UI CCWGG 3 cut(s) 49, 275, 361
Bst4CI ACNGT 3 cut(s) 519, 785, 1031
BstBAI YACGTR 1 cut(s) 999
BstBI TTCGAA 2 cut(s) 451, 858
BstC8I GCNNGC 2 cut(s) 127, 642
BstDEI CTNAG 6 cut(s) 70, 113, 242, 375, 389, 816
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 4 cut(s) 381, 905, 1045, 1135
BstFNI CGCG 2 cut(s) 228, 828
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 3 cut(s) 270, 830, 935
BstKTI GATC 7 cut(s) 67, 93, 252, 490, 589, 610, 897
BstMAI GTCTC 2 cut(s) 211, 371
BstMBI GATC 7 cut(s) 64, 90, 249, 487, 586, 607, 894
BstMCI CGRYCG 1 cut(s) 897
BstMWI GCNNNNNNNGC 4 cut(s) 131, 265, 276, 761
BstNI CCWGG 3 cut(s) 49, 275, 361
BstSCI CCNGG 3 cut(s) 47, 273, 359
BstUI CGCG 2 cut(s) 228, 828
BstV1I GCAGC 1 cut(s) 109
BsuI GTATCC 1 cut(s) 504
BsuRI GGCC 4 cut(s) 216, 462, 676, 956
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 4 cut(s) 381, 905, 1045, 1135
BtsI GCAGTG 1 cut(s) 971
BtsIMutI CAGTG 2 cut(s) 393, 971
Cac8I GCNNGC 2 cut(s) 127, 642
CfoI GCGC 3 cut(s) 270, 830, 935
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 215
Cfr42I CCGCGG 1 cut(s) 229
Csp6I GTAC 2 cut(s) 82, 786
CviAII CATG 6 cut(s) 35, 79, 464, 482, 928, 978
CviQI GTAC 2 cut(s) 82, 786
DdeI CTNAG 6 cut(s) 70, 113, 242, 375, 389, 816
DpnI GATC 7 cut(s) 66, 92, 251, 489, 588, 609, 896
DpnII GATC 7 cut(s) 64, 90, 249, 487, 586, 607, 894
EaeI YGGCCR 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 597
Eco147I AGGCCT 1 cut(s) 676
Eco24I GRGCYC 2 cut(s) 281, 303
Eco31I GGTCTC 1 cut(s) 371
Eco88I CYCGRG 1 cut(s) 170
EcoRI GAATTC 2 cut(s) 650, 854
EcoRII CCWGG 3 cut(s) 47, 273, 359
EcoT14I CCWWGG 1 cut(s) 597
EcoT22I ATGCAT 1 cut(s) 720
EcoT38I GRGCYC 2 cut(s) 281, 303
ErhI CCWWGG 1 cut(s) 597
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 6 cut(s) 38, 82, 467, 485, 931, 981
FaqI GGGAC 1 cut(s) 140
FatI CATG 6 cut(s) 34, 78, 463, 481, 927, 977
FauI CCCGC 3 cut(s) 165, 221, 748
FauNDI CATATG 1 cut(s) 666
Fnu4HI GCNGC 1 cut(s) 123
FokI GGATG 4 cut(s) 368, 892, 1052, 1142
FriOI GRGCYC 2 cut(s) 281, 303
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 2 cut(s) 354, 407
GlaI GCGC 3 cut(s) 269, 829, 934
GluI GCNGC 1 cut(s) 123
GsuI CTGGAG 1 cut(s) 302
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 4 cut(s) 216, 462, 676, 956
HapII CCGG 1 cut(s) 126
HhaI GCGC 3 cut(s) 270, 830, 935
Hin1II CATG 6 cut(s) 38, 82, 467, 485, 931, 981
Hin6I GCGC 3 cut(s) 268, 828, 933
HinP1I GCGC 3 cut(s) 268, 828, 933
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HindIII AAGCTT 2 cut(s) 818, 862
HinfI GANTC 4 cut(s) 108, 153, 313, 617
HpaII CCGG 1 cut(s) 126
HphI GGTGA 2 cut(s) 31, 985
Hpy166II GTNNAC 3 cut(s) 312, 788, 1027
Hpy188I TCNGA 4 cut(s) 152, 245, 376, 591
Hpy188III TCNNGA 7 cut(s) 20, 172, 291, 436, 572, 898, 1024
Hpy8I GTNNAC 3 cut(s) 312, 788, 1027
Hpy99I CGWCG 2 cut(s) 828, 923
HpyAV CCTTC 1 cut(s) 1120
HpyCH4III ACNGT 3 cut(s) 519, 785, 1031
HpyCH4IV ACGT 1 cut(s) 998
HpyCH4V TGCA 3 cut(s) 144, 718, 764
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 265, 276, 761
HpyF3I CTNAG 6 cut(s) 70, 113, 242, 375, 389, 816
HpySE526I ACGT 1 cut(s) 998
Hsp92II CATG 6 cut(s) 38, 82, 467, 485, 931, 981
HspAI GCGC 3 cut(s) 268, 828, 933
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 7 cut(s) 64, 90, 249, 487, 586, 607, 894
LmnI GCTCC 3 cut(s) 256, 702, 890
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 2 cut(s) 153, 891
MaeI CTAG 2 cut(s) 354, 407
MaeII ACGT 1 cut(s) 998
MaeIII GTNAC 4 cut(s) 37, 368, 1031, 1055
MalI GATC 7 cut(s) 66, 92, 251, 489, 588, 609, 896
MboI GATC 7 cut(s) 64, 90, 249, 487, 586, 607, 894
MboII GAAGA 7 cut(s) 35, 100, 139, 530, 617, 646, 789
MhlI GDGCHC 4 cut(s) 261, 281, 303, 579
MlsI TGGCCA 1 cut(s) 462
MluCI AATT 7 cut(s) 10, 650, 854, 878, 1075, 1098, 1166
MluNI TGGCCA 1 cut(s) 462
MlyI GAGTC 4 cut(s) 102, 162, 307, 611
Mox20I TGGCCA 1 cut(s) 462
Mph1103I ATGCAT 1 cut(s) 720
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 1 cut(s) 1169
MscI TGGCCA 1 cut(s) 462
MseI TTAA 4 cut(s) 498, 849, 1113, 1192
MslI CAYNNNNRTG 3 cut(s) 33, 500, 582
Msp20I TGGCCA 1 cut(s) 462
MspA1I CMGCKG 2 cut(s) 228, 755
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 3 cut(s) 49, 275, 361
MvaI CCWGG 3 cut(s) 49, 275, 361
MvnI CGCG 2 cut(s) 228, 828
MwoI GCNNNNNNNGC 4 cut(s) 131, 265, 276, 761
NaeI GCCGGC 1 cut(s) 127
NdeI CATATG 1 cut(s) 666
NdeII GATC 7 cut(s) 64, 90, 249, 487, 586, 607, 894
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 6 cut(s) 38, 82, 467, 485, 931, 981
NlaIV GGNNCC 1 cut(s) 217
NmuCI GTSAC 2 cut(s) 37, 1055
NsiI ATGCAT 1 cut(s) 720
NspV TTCGAA 2 cut(s) 451, 858
PaeR7I CTCGAG 1 cut(s) 170
PceI AGGCCT 1 cut(s) 676
PcsI WCGNNNNNNNCGW 1 cut(s) 1020
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 1 cut(s) 1169
PflFI GACNNNGTC 1 cut(s) 683
PkrI GCNGC 1 cut(s) 124
Ple19I CGATCG 1 cut(s) 897
PleI GAGTC 4 cut(s) 102, 161, 307, 611
PpsI GAGTC 4 cut(s) 102, 161, 307, 611
Ppu21I YACGTR 1 cut(s) 999
PshBI ATTAAT 1 cut(s) 498
Psp6I CCWGG 3 cut(s) 47, 273, 359
PspGI CCWGG 3 cut(s) 47, 273, 359
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 215
PsyI GACNNNGTC 1 cut(s) 683
PvuI CGATCG 1 cut(s) 897
RsaI GTAC 2 cut(s) 83, 787
RsaNI GTAC 2 cut(s) 82, 786
RseI CAYNNNNRTG 3 cut(s) 33, 500, 582
SacII CCGCGG 1 cut(s) 229
SaqAI TTAA 4 cut(s) 498, 849, 1113, 1192
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 7 cut(s) 64, 90, 249, 487, 586, 607, 894
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 4 cut(s) 102, 162, 307, 611
ScrFI CCNGG 3 cut(s) 49, 275, 361
SduI GDGCHC 4 cut(s) 261, 281, 303, 579
SfaNI GCATC 2 cut(s) 153, 891
Sfr274I CTCGAG 1 cut(s) 170
Sfr303I CCGCGG 1 cut(s) 229
SfuI TTCGAA 2 cut(s) 451, 858
SgrBI CCGCGG 1 cut(s) 229
SlaI CTCGAG 1 cut(s) 170
SmiMI CAYNNNNRTG 3 cut(s) 33, 500, 582
SmlI CTYRAG 3 cut(s) 170, 545, 572
SmoI CTYRAG 3 cut(s) 170, 545, 572
Sse9I AATT 7 cut(s) 10, 650, 854, 878, 1075, 1098, 1166
SseBI AGGCCT 1 cut(s) 676
SsiI CCGC 5 cut(s) 158, 226, 228, 736, 755
SspMI CTAG 2 cut(s) 354, 407
StuI AGGCCT 1 cut(s) 676
StyD4I CCNGG 3 cut(s) 47, 273, 359
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 3 cut(s) 519, 785, 1031
TaiI ACGT 1 cut(s) 1001
TasI AATT 7 cut(s) 10, 650, 854, 878, 1075, 1098, 1166
TatI WGTACW 1 cut(s) 785
Tru1I TTAA 4 cut(s) 498, 849, 1113, 1192
Tru9I TTAA 4 cut(s) 498, 849, 1113, 1192
TscAI CASTG 2 cut(s) 400, 971
TseFI GTSAC 2 cut(s) 37, 1055
TseI GCWGC 1 cut(s) 122
Tsp45I GTSAC 2 cut(s) 37, 1055
TspDTI ATGAA 4 cut(s) 71, 916, 1099, 1172
TspRI CASTG 2 cut(s) 400, 971
Tth111I GACNNNGTC 1 cut(s) 683
VspI ATTAAT 1 cut(s) 498
XapI RAATTY 3 cut(s) 650, 854, 878
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 170
XmnI GAANNNNTTC 1 cut(s) 1169
XspI CTAG 2 cut(s) 354, 407
Zsp2I ATGCAT 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.