FvH4_6g48360

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001784
Physical Location & Seq
Reverse (-)
36419994 .. 36420491
498 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g48360.t1

Sequence Viewer

Length: 498 bp
ATGTCTAGTAACGAAGCAAACAGCAGTGATGACTTGACCCTACAGTCAGTACAGGAATCTCACTCCCGCCAAGCCTGGCCAGCATTGTACAGTTTTTCTTTTGGACAGCTACCTGCGGGCAGCATCAATCAGACTCCTATGTCGCGGCGGAAGGCATCCAAGGCGGCCATAGAGGCCATGCCAAAAGTGACAGTGGCATACGAGGATGGAAACGAATGCAGTATCTGCTTGAATGGCTTCAAGGTCGGTGACGAAGTCAAGGAGATGCCTTGCAAGCATAGCTTCCACGGTGCCTGTATCGAAAAGTGGATGGGAATGAGCACTCTTTGCCCGCTTTGCAGGTTCTCTTTGCCTGCAGAGTCAGAAGATGAACAAAACAGTGATAATAACAATTATCATATTGAATATAATATTATTTATAATATTGAATTTGGCTCGGAGGATGAAGAAGATACCGGAGGTGAATGGGATACAAGCTCTGTCGATGATGATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

166

Amino Acids

18.34

Weight (kDa)

4.32

Isoelectric Point (pI)

74.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 71 - 114 2e-15 Ring finger domain
zf-RING_11 PF17123 72 - 100 1e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 72 - 113 3.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 73 - 113 2.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 73 - 111 4.9e-06 RING-type zinc-finger
zf-rbx1 PF12678 89 - 114 9.5e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 420
AasI GACNNNNNNGTC 2 cut(s) 43, 139
Acc36I ACCTGC 2 cut(s) 121, 330
AccB1I GGYRCC 1 cut(s) 290
AccII CGCG 1 cut(s) 145
AciI CCGC 6 cut(s) 67, 116, 145, 148, 164, 332
AcoI YGGCCR 2 cut(s) 77, 165
AcsI RAATTY 1 cut(s) 428
AfaI GTAC 2 cut(s) 51, 89
AgsI TTSAA 4 cut(s) 232, 241, 404, 428
AjnI CCWGG 1 cut(s) 74
AluBI AGCT 3 cut(s) 109, 282, 477
AluI AGCT 3 cut(s) 109, 282, 477
Alw21I GWGCWC 1 cut(s) 323
AlwNI CAGNNNCTG 1 cut(s) 225
AoxI GGCC 3 cut(s) 77, 165, 174
ApeKI GCWGC 1 cut(s) 120
ApoI RAATTY 1 cut(s) 428
Asp700I GAANNNNTTC 1 cut(s) 236
AsuHPI GGTGA 2 cut(s) 260, 473
BalI TGGCCA 1 cut(s) 79
BanI GGYRCC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 323
BbvI GCAGC 1 cut(s) 132
BccI CCATC 2 cut(s) 200, 304
BciT130I CCWGG 1 cut(s) 76
BciVI GTATCC 1 cut(s) 463
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 2 cut(s) 41, 354
BfuAI ACCTGC 2 cut(s) 121, 330
BfuI GTATCC 1 cut(s) 463
BglI GCCNNNNNGGC 1 cut(s) 173
BisI GCNGC 3 cut(s) 121, 146, 165
BlsI GCNGC 3 cut(s) 122, 147, 166
Bme1390I CCNGG 1 cut(s) 76
BmiI GGNNCC 1 cut(s) 292
BmrFI CCNGG 1 cut(s) 76
BmsI GCATC 3 cut(s) 132, 164, 255
BsaJI CCNNGG 2 cut(s) 159, 286
BsaWI WCCGGW 1 cut(s) 455
BseBI CCWGG 1 cut(s) 76
BseDI CCNNGG 2 cut(s) 159, 286
BseGI GGATG 4 cut(s) 155, 211, 315, 448
BseXI GCAGC 1 cut(s) 132
Bsh1236I CGCG 1 cut(s) 145
BshFI GGCC 3 cut(s) 79, 167, 176
BshNI GGYRCC 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 323
BsiSI CCGG 1 cut(s) 456
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 3 cut(s) 79, 167, 176
Bsp1286I GDGCHC 1 cut(s) 323
Bsp1407I TGTACA 1 cut(s) 87
BspACI CCGC 6 cut(s) 67, 116, 145, 148, 164, 332
BspANI GGCC 3 cut(s) 79, 167, 176
BspFNI CGCG 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 292
BspMAI CTGCAG 1 cut(s) 358
BspMI ACCTGC 2 cut(s) 121, 330
BspT107I GGYRCC 1 cut(s) 290
BsrGI TGTACA 1 cut(s) 87
BssECI CCNNGG 2 cut(s) 159, 286
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 76
Bst4CI ACNGT 5 cut(s) 45, 92, 193, 290, 380
BstAPI GCANNNNNTGC 2 cut(s) 225, 327
BstAUI TGTACA 1 cut(s) 87
BstC8I GCNNGC 5 cut(s) 81, 118, 275, 332, 354
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 4 cut(s) 155, 211, 315, 448
BstFNI CGCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 9 cut(s) 80, 161, 173, 225, 234, 274, 279, 327, 336
BstNI CCWGG 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 74
BstSFI CTRYAG 2 cut(s) 41, 354
BstUI CGCG 1 cut(s) 145
BstV1I GCAGC 1 cut(s) 132
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 3 cut(s) 79, 167, 176
BtgI CCRYGG 1 cut(s) 286
BtsCI GGATG 4 cut(s) 155, 211, 315, 448
BtsI GCAGTG 1 cut(s) 31
BtsIMutI CAGTG 3 cut(s) 31, 198, 385
BveI ACCTGC 2 cut(s) 121, 330
Cac8I GCNNGC 5 cut(s) 81, 118, 275, 332, 354
CaiI CAGNNNCTG 1 cut(s) 225
Csp6I GTAC 2 cut(s) 50, 88
CviAII CATG 1 cut(s) 178
CviJI RGCY 9 cut(s) 74, 79, 109, 167, 176, 237, 282, 435, 477
CviKI_1 RGCY 9 cut(s) 74, 79, 109, 167, 176, 237, 282, 435, 477
CviQI GTAC 2 cut(s) 50, 88
DrdI GACNNNNNNGTC 2 cut(s) 43, 139
DseDI GACNNNNNNGTC 2 cut(s) 43, 139
EaeI YGGCCR 2 cut(s) 77, 165
EciI GGCGGA 1 cut(s) 163
Eco130I CCWWGG 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 1 cut(s) 181
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FatI CATG 1 cut(s) 177
FauI CCCGC 3 cut(s) 74, 109, 339
Fnu4HI GCNGC 3 cut(s) 121, 146, 165
FokI GGATG 4 cut(s) 142, 218, 322, 455
Fsp4HI GCNGC 3 cut(s) 121, 146, 165
FspBI CTAG 1 cut(s) 6
GluI GCNGC 3 cut(s) 121, 146, 165
HaeIII GGCC 3 cut(s) 79, 167, 176
HapII CCGG 1 cut(s) 456
Hin1II CATG 1 cut(s) 181
HinfI GANTC 3 cut(s) 56, 133, 359
HpaII CCGG 1 cut(s) 456
HphI GGTGA 2 cut(s) 260, 473
Hpy188I TCNGA 3 cut(s) 132, 364, 439
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 5 cut(s) 45, 92, 193, 290, 380
HpyCH4V TGCA 4 cut(s) 219, 273, 339, 356
HpyF10VI GCNNNNNNNGC 9 cut(s) 80, 161, 173, 225, 234, 274, 279, 327, 336
Hsp92II CATG 1 cut(s) 181
LpnPI CCDG 9 cut(s) 38, 61, 88, 93, 126, 307, 325, 366, 469
Lsp1109I GCAGC 1 cut(s) 132
LweI GCATC 3 cut(s) 132, 164, 255
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 3 cut(s) 8, 187, 248
MboII GAAGA 3 cut(s) 377, 458, 461
MhlI GDGCHC 1 cut(s) 323
MlsI TGGCCA 1 cut(s) 79
MluCI AATT 2 cut(s) 391, 428
MluNI TGGCCA 1 cut(s) 79
MlyI GAGTC 2 cut(s) 127, 368
MnlI CCTC 4 cut(s) 166, 196, 433, 452
Mox20I TGGCCA 1 cut(s) 79
MroXI GAANNNNTTC 1 cut(s) 236
MscI TGGCCA 1 cut(s) 79
Msp20I TGGCCA 1 cut(s) 79
MspI CCGG 1 cut(s) 456
MspR9I CCNGG 1 cut(s) 76
Mva1269I GAATGC 1 cut(s) 221
MvaI CCWGG 1 cut(s) 76
MvnI CGCG 1 cut(s) 145
MwoI GCNNNNNNNGC 9 cut(s) 80, 161, 173, 225, 234, 274, 279, 327, 336
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 1 cut(s) 292
NmuCI GTSAC 2 cut(s) 187, 248
PctI GAATGC 1 cut(s) 221
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 1 cut(s) 56
PflFI GACNNNGTC 1 cut(s) 254
PkrI GCNGC 3 cut(s) 122, 147, 166
PleI GAGTC 2 cut(s) 127, 367
PpsI GAGTC 2 cut(s) 127, 367
PsiI TTATAA 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 292
PstI CTGCAG 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 225
PsyI GACNNNGTC 1 cut(s) 254
RsaI GTAC 2 cut(s) 51, 89
RsaNI GTAC 2 cut(s) 50, 88
SatI GCNGC 3 cut(s) 121, 146, 165
SchI GAGTC 2 cut(s) 127, 368
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 1 cut(s) 323
SetI ASST 7 cut(s) 111, 115, 246, 284, 344, 463, 479
SfaNI GCATC 3 cut(s) 132, 164, 255
SfcI CTRYAG 2 cut(s) 41, 354
SfiI GGCCNNNNNGGCC 1 cut(s) 173
Sse9I AATT 2 cut(s) 391, 428
SsiI CCGC 6 cut(s) 67, 116, 145, 148, 164, 332
SspI AATATT 2 cut(s) 412, 424
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 74
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 5 cut(s) 45, 92, 193, 290, 380
TaqI TCGA 2 cut(s) 300, 483
TasI AATT 2 cut(s) 391, 428
TatI WGTACW 2 cut(s) 49, 87
TauI GCSGC 2 cut(s) 148, 167
TfiI GAWTC 1 cut(s) 56
TscAI CASTG 3 cut(s) 31, 198, 385
TseFI GTSAC 2 cut(s) 187, 248
TseI GCWGC 1 cut(s) 120
Tsp45I GTSAC 2 cut(s) 187, 248
TspDTI ATGAA 2 cut(s) 384, 459
TspRI CASTG 3 cut(s) 31, 198, 385
Tth111I GACNNNGTC 1 cut(s) 254
XapI RAATTY 1 cut(s) 428
XmnI GAANNNNTTC 1 cut(s) 236
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.