RLG00000021762

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001784
Physical Location & Seq
Reverse (-)
78028050 .. 78029848
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021762

Sequence Viewer

Length: 525 bp
ATGTCCCACCAAAATCTAGTTCCTTCTGGCCCGCCCATCACAGTCATCCCTGCAGTACGCATCAATGTTCAATTTGAACTCGAACGTGCTAACATAGGTCGGAACAACAACCGAGGGCCGCTAGTGCTGCCGGCATCCAAGGCCTCCATAGACGCCATGGCGAAAGTGGAAGTCACGTCAGAGGCAGGGCCATCGGAGTGCAGTATCTGCTTGGAAGTCTTGAAGGTGGGGTGTGAAGCCAATGAGATGCCTAATGTAAGCACAGGTTTCACCCGGCTTGTATTGAAACCTCGCTTCTCCGGCACGCTTCATGCCCGCTTTGCAGGTAAAGTCTGCGCTGAAGCCAAAGATATGCCTTGTAAGCACAGGTTTCACTCGCACTGTATTGAAAACTGGCTGTACAGGCGTGCTTCCTGCCCACTTTGCAGGTTCACCATGCCCGTTGACGTCGAAGAAGAGACGAACGGCATGAGCGAACATTGGATTGATATCACGGATTTTTTGGATTTTAGGGATATGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

175

Amino Acids

19.42

Weight (kDa)

6.07

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-ANAPC11 PF12861 106 - 147 3.7e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-rbx1 PF12678 112 - 143 5e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 115 - 143 1.5e-08 Ring finger domain
zf-C3HC4 PF00097 117 - 142 7.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 118 - 142 1e-05 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 450
Acc36I ACCTGC 2 cut(s) 314, 417
AciI CCGC 3 cut(s) 32, 119, 316
AcuI CTGAAG 1 cut(s) 360
AcyI GRCGYC 2 cut(s) 153, 447
AfaI GTAC 2 cut(s) 57, 401
AgsI TTSAA 5 cut(s) 71, 77, 223, 286, 389
AjiI CACGTC 1 cut(s) 177
AjuI GAANNNNNNNTTGG 1 cut(s) 35
Alw26I GTCTC 1 cut(s) 452
AlwNI CAGNNNCTG 1 cut(s) 207
AoxI GGCC 4 cut(s) 28, 116, 141, 188
ApeKI GCWGC 1 cut(s) 127
AspLEI GCGC 1 cut(s) 338
AspS9I GGNCC 3 cut(s) 29, 116, 188
AsuC2I CCSGG 1 cut(s) 274
AsuHPI GGTGA 2 cut(s) 262, 424
BbvI GCAGC 1 cut(s) 114
BccI CCATC 2 cut(s) 44, 199
BceAI ACGGC 1 cut(s) 481
BcgI CGANNNNNNTGC 2 cut(s) 174, 208
BcnI CCSGG 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 2 cut(s) 17, 122
BfmI CTRYAG 1 cut(s) 51
BfuAI ACCTGC 2 cut(s) 314, 417
BisI GCNGC 2 cut(s) 119, 128
BlsI GCNGC 2 cut(s) 120, 129
Bme1390I CCNGG 1 cut(s) 274
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 3 cut(s) 29, 116, 188
BmrFI CCNGG 1 cut(s) 274
BmsI GCATC 3 cut(s) 69, 143, 237
BpuMI CCSGG 1 cut(s) 274
BsaBI GATNNNNATC 1 cut(s) 488
BsaHI GRCGYC 2 cut(s) 153, 447
BsaJI CCNNGG 3 cut(s) 112, 138, 156
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 398
Bse8I GATNNNNATC 1 cut(s) 488
BseDI CCNNGG 3 cut(s) 112, 138, 156
BseGI GGATG 2 cut(s) 45, 134
BseJI GATNNNNATC 1 cut(s) 488
BseNI ACTGG 1 cut(s) 398
BseXI GCAGC 1 cut(s) 114
BsgI GTGCAG 1 cut(s) 220
BshFI GGCC 4 cut(s) 30, 118, 143, 190
BsiSI CCGG 3 cut(s) 131, 274, 300
BsmAI GTCTC 1 cut(s) 452
BsmBI CGTCTC 1 cut(s) 452
BsnI GGCC 4 cut(s) 30, 118, 143, 190
Bsp1407I TGTACA 1 cut(s) 399
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 3 cut(s) 32, 119, 316
BspANI GGCC 4 cut(s) 30, 118, 143, 190
BspMAI CTGCAG 1 cut(s) 55
BspMI ACCTGC 2 cut(s) 314, 417
BsrFI RCCGGY 1 cut(s) 130
BsrGI TGTACA 1 cut(s) 399
BsrI ACTGG 1 cut(s) 398
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 3 cut(s) 112, 138, 156
BssNI GRCGYC 2 cut(s) 153, 447
BssT1I CCWWGG 2 cut(s) 138, 156
Bst4CI ACNGT 2 cut(s) 43, 383
Bst6I CTCTTC 1 cut(s) 450
BstACI GRCGYC 2 cut(s) 153, 447
BstAPI GCANNNNNTGC 1 cut(s) 207
BstAUI TGTACA 1 cut(s) 399
BstC8I GCNNGC 5 cut(s) 32, 132, 305, 316, 408
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 2 cut(s) 45, 134
BstHHI GCGC 1 cut(s) 338
BstMAI GTCTC 1 cut(s) 452
BstMWI GCNNNNNNNGC 9 cut(s) 124, 127, 140, 207, 300, 320, 361, 403, 423
BstSCI CCNGG 1 cut(s) 272
BstSFI CTRYAG 1 cut(s) 51
BstV1I GCAGC 1 cut(s) 114
BsuRI GGCC 4 cut(s) 30, 118, 143, 190
BtgI CCRYGG 1 cut(s) 156
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 2 cut(s) 45, 134
BtsIMutI CAGTG 1 cut(s) 379
BveI ACCTGC 2 cut(s) 314, 417
Cac8I GCNNGC 5 cut(s) 32, 132, 305, 316, 408
CaiI CAGNNNCTG 1 cut(s) 207
CfoI GCGC 1 cut(s) 338
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 3 cut(s) 29, 116, 188
CseI GACGC 1 cut(s) 161
Csp6I GTAC 2 cut(s) 56, 400
CviAII CATG 4 cut(s) 157, 311, 436, 469
CviJI RGCY 8 cut(s) 30, 118, 143, 190, 239, 277, 344, 397
CviKI_1 RGCY 8 cut(s) 30, 118, 143, 190, 239, 277, 344, 397
CviQI GTAC 2 cut(s) 56, 400
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
Eco130I CCWWGG 2 cut(s) 138, 156
Eco147I AGGCCT 1 cut(s) 143
Eco32I GATATC 1 cut(s) 490
Eco57I CTGAAG 1 cut(s) 360
EcoRV GATATC 1 cut(s) 490
EcoT14I CCWWGG 2 cut(s) 138, 156
ErhI CCWWGG 2 cut(s) 138, 156
Esp3I CGTCTC 1 cut(s) 452
FaeI CATG 4 cut(s) 160, 314, 439, 472
FaiI YATR 8 cut(s) 95, 149, 158, 312, 353, 437, 470, 518
FatI CATG 4 cut(s) 156, 310, 435, 468
FauI CCCGC 2 cut(s) 39, 323
Fnu4HI GCNGC 2 cut(s) 119, 128
FokI GGATG 2 cut(s) 32, 121
Fsp4HI GCNGC 2 cut(s) 119, 128
FspBI CTAG 2 cut(s) 17, 122
GlaI GCGC 1 cut(s) 337
GluI GCNGC 2 cut(s) 119, 128
HaeIII GGCC 4 cut(s) 30, 118, 143, 190
HapII CCGG 3 cut(s) 131, 274, 300
HgaI GACGC 1 cut(s) 161
HhaI GCGC 1 cut(s) 338
Hin1I GRCGYC 2 cut(s) 153, 447
Hin1II CATG 4 cut(s) 160, 314, 439, 472
Hin6I GCGC 1 cut(s) 336
HinP1I GCGC 1 cut(s) 336
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HpaII CCGG 3 cut(s) 131, 274, 300
HphI GGTGA 2 cut(s) 262, 424
Hpy166II GTNNAC 2 cut(s) 432, 445
Hpy188I TCNGA 3 cut(s) 102, 181, 196
Hpy188III TCNNGA 1 cut(s) 220
Hpy8I GTNNAC 2 cut(s) 432, 445
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 2 cut(s) 33, 217
HpyCH4III ACNGT 2 cut(s) 43, 383
HpyCH4IV ACGT 3 cut(s) 85, 176, 447
HpyCH4V TGCA 4 cut(s) 53, 201, 323, 426
HpyF10VI GCNNNNNNNGC 9 cut(s) 124, 127, 140, 207, 300, 320, 361, 403, 423
HpySE526I ACGT 3 cut(s) 85, 176, 447
Hsp92I GRCGYC 2 cut(s) 153, 447
Hsp92II CATG 4 cut(s) 160, 314, 439, 472
HspAI GCGC 1 cut(s) 336
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Lsp1109I GCAGC 1 cut(s) 114
LweI GCATC 3 cut(s) 69, 143, 237
MaeI CTAG 2 cut(s) 17, 122
MaeII ACGT 3 cut(s) 85, 176, 447
MaeIII GTNAC 1 cut(s) 172
MboII GAAGA 2 cut(s) 464, 467
MluCI AATT 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 4 cut(s) 107, 154, 175, 300
MroNI GCCGGC 1 cut(s) 130
MslI CAYNNNNRTG 1 cut(s) 196
MspI CCGG 3 cut(s) 131, 274, 300
MspR9I CCNGG 1 cut(s) 274
MwoI GCNNNNNNNGC 9 cut(s) 124, 127, 140, 207, 300, 320, 361, 403, 423
NaeI GCCGGC 1 cut(s) 132
NciI CCSGG 1 cut(s) 274
NcoI CCATGG 1 cut(s) 156
NgoMIV GCCGGC 1 cut(s) 130
NlaIII CATG 4 cut(s) 160, 314, 439, 472
NmuCI GTSAC 1 cut(s) 172
PceI AGGCCT 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 471
PdiI GCCGGC 1 cut(s) 132
PkrI GCNGC 2 cut(s) 120, 129
PspPI GGNCC 3 cut(s) 29, 116, 188
PstI CTGCAG 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 207
RsaI GTAC 2 cut(s) 57, 401
RsaNI GTAC 2 cut(s) 56, 400
RseI CAYNNNNRTG 1 cut(s) 196
SatI GCNGC 2 cut(s) 119, 128
Sau96I GGNCC 3 cut(s) 29, 116, 188
ScrFI CCNGG 1 cut(s) 274
SfaNI GCATC 3 cut(s) 69, 143, 237
SfcI CTRYAG 1 cut(s) 51
SmiMI CAYNNNNRTG 1 cut(s) 196
Sse9I AATT 1 cut(s) 71
SseBI AGGCCT 1 cut(s) 143
SsiI CCGC 3 cut(s) 32, 119, 316
SspMI CTAG 2 cut(s) 17, 122
StuI AGGCCT 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 272
StyI CCWWGG 2 cut(s) 138, 156
TaaI ACNGT 2 cut(s) 43, 383
TaiI ACGT 3 cut(s) 88, 179, 450
TaqI TCGA 2 cut(s) 81, 450
TasI AATT 1 cut(s) 71
TatI WGTACW 1 cut(s) 399
TauI GCSGC 1 cut(s) 121
TscAI CASTG 1 cut(s) 386
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 299
TspGWI ACGGA 1 cut(s) 509
TspRI CASTG 1 cut(s) 386
XcmI CCANNNNNNNNNTGG 2 cut(s) 154, 163
XspI CTAG 2 cut(s) 17, 122
ZraI GACGTC 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.