Rh2CG582300

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001784
Physical Location & Seq
Reverse (-)
76052076 .. 76054322
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG582300.1

Sequence Viewer

Length: 552 bp
ATGTCCCAGTCAATCTCTTCCGTCACAGAAGAAGGGACGACGGAATATGACGATCATTGCACTATTTGCTTGGAAGACTTGATTTCCAAGGTGAGGGAGATGCCTTGTAAGCATAGGTTTCACTCCCCTTGTATCGAGTCTTGGCTGCTCAGAAGTGAATCCTGCCCTCTTTGCAGGAGGCCGTTTCTGAGTGGCAGCAATCTCAGTCGTAGACTTTGGCGATTAGTTTCCGGCAGTGAAAACGGCGTCGTGGTGCGCACTGAGATGGAAAACGAACATGAAGTTACATCGTTCTATTCAATCCCCGATGAAATACCAGAGTTCTTGTTCCATCTAACGCTTTTCTGGGTCATCCAGCCATGGACAAAAAATATCAGCACCTACACCCATCTTCAAACACATGAACGGAACTCTCACTGCAAAAATGAGTCCCAAAGAACATGCCATCAGAAAATTCCTCCCACATACTTAGAAATTGGAACGCTTCCATGGATTCTCCGGAGTCCACACAAGACCCGGCCCCCAATTATTGTATTCCACATGACCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

183

Amino Acids

21.47

Weight (kDa)

6.59

Isoelectric Point (pI)

73.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 15 - 59 9.6e-08 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 17 - 62 4.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 19 - 59 7e-12 Ring finger domain
zf-C3HC4 PF00097 20 - 58 7.9e-11 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 20 - 58 9.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 20 - 45 6.1e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 257
AccI GTMKAC 1 cut(s) 211
AccIII TCCGGA 1 cut(s) 498
AcsI RAATTY 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 246
AfiI CCNNNNNNNGG 1 cut(s) 93
AgsI TTSAA 2 cut(s) 300, 395
Aor13HI TCCGGA 1 cut(s) 498
AoxI GGCC 2 cut(s) 179, 518
ApeKI GCWGC 2 cut(s) 145, 195
ApoI RAATTY 1 cut(s) 453
AspLEI GCGC 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 519
AsuC2I CCSGG 1 cut(s) 517
AsuHPI GGTGA 1 cut(s) 103
BbsI GAAGAC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 132, 207
BccI CCATC 4 cut(s) 259, 339, 396, 453
BceAI ACGGC 2 cut(s) 166, 259
BcnI CCSGG 1 cut(s) 517
BisI GCNGC 2 cut(s) 146, 196
BlsI GCNGC 2 cut(s) 147, 197
Bme1390I CCNGG 1 cut(s) 517
BmgT120I GGNCC 1 cut(s) 519
BmiI GGNNCC 1 cut(s) 521
BmrFI CCNGG 1 cut(s) 517
BmsI GCATC 1 cut(s) 90
BpiI GAAGAC 1 cut(s) 81
BpuMI CCSGG 1 cut(s) 517
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 3 cut(s) 87, 359, 488
BsaWI WCCGGW 1 cut(s) 498
Bsc4I CCNNNNNNNGG 1 cut(s) 93
Bse1I ACTGG 1 cut(s) 7
Bse3DI GCAATG 1 cut(s) 55
BseAI TCCGGA 1 cut(s) 498
BseDI CCNNGG 3 cut(s) 87, 359, 488
BseGI GGATG 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 93
BseMI GCAATG 1 cut(s) 55
BseMII CTCAG 4 cut(s) 163, 179, 217, 252
BseNI ACTGG 1 cut(s) 7
BseXI GCAGC 2 cut(s) 132, 207
BshFI GGCC 2 cut(s) 181, 520
BsiSI CCGG 3 cut(s) 231, 499, 517
BslFI GGGAC 2 cut(s) 49, 415
BslI CCNNNNNNNGG 1 cut(s) 93
BsmFI GGGAC 2 cut(s) 49, 415
BsnI GGCC 2 cut(s) 181, 520
Bsp13I TCCGGA 1 cut(s) 498
Bsp143I GATC 1 cut(s) 52
Bsp19I CCATGG 2 cut(s) 359, 488
BspANI GGCC 2 cut(s) 181, 520
BspCNI CTCAG 4 cut(s) 162, 180, 216, 253
BspEI TCCGGA 1 cut(s) 498
BspLI GGNNCC 1 cut(s) 521
BsrDI GCAATG 1 cut(s) 55
BsrI ACTGG 1 cut(s) 7
BssECI CCNNGG 3 cut(s) 87, 359, 488
BssMI GATC 1 cut(s) 52
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 3 cut(s) 87, 359, 488
Bst6I CTCTTC 1 cut(s) 22
BstACI GRCGYC 1 cut(s) 246
BstAPI GCANNNNNTGC 1 cut(s) 66
BstDEI CTNAG 5 cut(s) 149, 188, 203, 261, 469
BstDSI CCRYGG 2 cut(s) 359, 488
BstF5I GGATG 1 cut(s) 351
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 1 cut(s) 55
BstMBI GATC 1 cut(s) 52
BstMWI GCNNNNNNNGC 3 cut(s) 66, 109, 171
BstNSI RCATGY 1 cut(s) 444
BstSCI CCNGG 1 cut(s) 515
BstV1I GCAGC 2 cut(s) 132, 207
BstV2I GAAGAC 1 cut(s) 81
BsuRI GGCC 2 cut(s) 181, 520
BtgI CCRYGG 2 cut(s) 359, 488
BtsCI GGATG 1 cut(s) 351
BtsI GCAGTG 2 cut(s) 241, 415
BtsIMutI CAGTG 3 cut(s) 241, 258, 415
CfoI GCGC 1 cut(s) 258
Cfr13I GGNCC 1 cut(s) 519
CseI GACGC 1 cut(s) 235
CviAII CATG 6 cut(s) 278, 360, 401, 441, 489, 541
CviJI RGCY 4 cut(s) 145, 181, 358, 520
CviKI_1 RGCY 4 cut(s) 145, 181, 358, 520
DdeI CTNAG 5 cut(s) 149, 188, 203, 261, 469
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 22
EarI CTCTTC 1 cut(s) 22
Eco130I CCWWGG 3 cut(s) 87, 359, 488
EcoT14I CCWWGG 3 cut(s) 87, 359, 488
ErhI CCWWGG 3 cut(s) 87, 359, 488
FaeI CATG 6 cut(s) 281, 363, 404, 444, 492, 544
FaiI YATR 9 cut(s) 48, 114, 279, 361, 402, 442, 466, 490, 542
FaqI GGGAC 2 cut(s) 49, 415
FatI CATG 6 cut(s) 277, 359, 400, 440, 488, 540
FblI GTMKAC 1 cut(s) 211
Fnu4HI GCNGC 2 cut(s) 146, 196
FokI GGATG 1 cut(s) 338
Fsp4HI GCNGC 2 cut(s) 146, 196
FspAI RTGCGCAY 1 cut(s) 257
FspI TGCGCA 1 cut(s) 257
GlaI GCGC 1 cut(s) 257
GluI GCNGC 2 cut(s) 146, 196
HaeIII GGCC 2 cut(s) 181, 520
HapII CCGG 3 cut(s) 231, 499, 517
HgaI GACGC 1 cut(s) 235
HhaI GCGC 1 cut(s) 258
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 6 cut(s) 281, 363, 404, 444, 492, 544
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HinfI GANTC 5 cut(s) 137, 158, 428, 493, 502
HpaII CCGG 3 cut(s) 231, 499, 517
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 2 cut(s) 212, 506
Hpy188I TCNGA 3 cut(s) 152, 189, 450
Hpy188III TCNNGA 1 cut(s) 499
Hpy8I GTNNAC 2 cut(s) 212, 506
Hpy99I CGWCG 2 cut(s) 43, 251
HpyAV CCTTC 1 cut(s) 26
HpyCH4V TGCA 3 cut(s) 60, 174, 420
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 109, 171
HpyF3I CTNAG 5 cut(s) 149, 188, 203, 261, 469
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 6 cut(s) 281, 363, 404, 444, 492, 544
HspAI GCGC 1 cut(s) 256
Kpn2I TCCGGA 1 cut(s) 498
Kzo9I GATC 1 cut(s) 52
LpnPI CCDG 9 cut(s) 20, 160, 175, 244, 330, 331, 368, 512, 530
Lsp1109I GCAGC 2 cut(s) 132, 207
LweI GCATC 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 22, 283
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 4 cut(s) 9, 41, 86, 383
MluCI AATT 4 cut(s) 453, 474, 525, 547
MlyI GAGTC 3 cut(s) 146, 437, 511
MnlI CCTC 4 cut(s) 87, 171, 177, 468
MroI TCCGGA 1 cut(s) 498
MseI TTAA 1 cut(s) 550
MslI CAYNNNNRTG 1 cut(s) 263
MspI CCGG 3 cut(s) 231, 499, 517
MspR9I CCNGG 1 cut(s) 517
MwoI GCNNNNNNNGC 3 cut(s) 66, 109, 171
NciI CCSGG 1 cut(s) 517
NcoI CCATGG 2 cut(s) 359, 488
NdeII GATC 1 cut(s) 52
NlaIII CATG 6 cut(s) 281, 363, 404, 444, 492, 544
NlaIV GGNNCC 1 cut(s) 521
NmuCI GTSAC 1 cut(s) 22
NsbI TGCGCA 1 cut(s) 257
NspI RCATGY 1 cut(s) 444
PfeI GAWTC 2 cut(s) 158, 493
PkrI GCNGC 2 cut(s) 147, 197
PleI GAGTC 3 cut(s) 145, 436, 510
PpsI GAGTC 3 cut(s) 145, 436, 510
PspN4I GGNNCC 1 cut(s) 521
PspPI GGNCC 1 cut(s) 519
RseI CAYNNNNRTG 1 cut(s) 263
SaqAI TTAA 1 cut(s) 550
SatI GCNGC 2 cut(s) 146, 196
Sau3AI GATC 1 cut(s) 52
Sau96I GGNCC 1 cut(s) 519
SchI GAGTC 3 cut(s) 146, 437, 511
ScrFI CCNGG 1 cut(s) 517
SetI ASST 3 cut(s) 93, 119, 383
SfaNI GCATC 1 cut(s) 90
SmiMI CAYNNNNRTG 1 cut(s) 263
Sse9I AATT 4 cut(s) 453, 474, 525, 547
StyD4I CCNGG 1 cut(s) 515
StyI CCWWGG 3 cut(s) 87, 359, 488
TaqI TCGA 1 cut(s) 135
TasI AATT 4 cut(s) 453, 474, 525, 547
TfiI GAWTC 2 cut(s) 158, 493
Tru1I TTAA 1 cut(s) 550
Tru9I TTAA 1 cut(s) 550
TscAI CASTG 3 cut(s) 241, 265, 422
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 2 cut(s) 145, 195
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 3 cut(s) 294, 324, 417
TspGWI ACGGA 3 cut(s) 10, 56, 421
TspRI CASTG 3 cut(s) 241, 265, 422
XapI RAATTY 1 cut(s) 453
XceI RCATGY 1 cut(s) 444
XmiI GTMKAC 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.