MD09G1052200.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001784
Physical Location & Seq
Forward (+)
3480452 .. 3480853
402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1052200.v1.1.491

Sequence Viewer

Length: 402 bp
ATGGCAGAAGTCGAAGCAGCATCCCAAACGTACAGTTTCCTCGCCTCTTATTTTTATCCCAATCCCCCGCCTGCCGCTGCCTTTGCGGCCATGGCGGGCCTGGCGTTGGTGACGGCCTTGGCTGGGGCCACCCAGGAGTCGATAGAAGCCTTGCCGAAGGTGACGGTGACGGAGGAAACAACGGCGGAGTGCAGTATCTGTTTGAAGGAGTTGGAGGTTGGGGAGGAAGCCAAGGAGATGCCATGTAACCATAGGTTTCATCCGGTTTGCATTGTTAGCTGGCTCGAGAGGCGTCCTTCCTGCCCGCTTTGCCGGTTTTCGATGCCTACGAGGGCGGAGGCGGCCGCACAAGGGCGAGGACATCAAACCACCGGGGTGGATTCGATTGAAATGGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

134

Amino Acids

14.31

Weight (kDa)

4.81

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 63 - 105 2e-14 Ring finger domain
zf-RING_11 PF17123 63 - 91 3.3e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 63 - 104 1.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 63 - 105 5.3e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 64 - 104 1.3e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 9 cut(s) 68, 75, 86, 95, 185, 305, 335, 341, 345
AcoI YGGCCR 2 cut(s) 87, 342
AcyI GRCGYC 1 cut(s) 292
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 3 cut(s) 106, 123, 351
AgsI TTSAA 2 cut(s) 205, 389
AjnI CCWGG 2 cut(s) 99, 132
AleI CACNNNNGTG 1 cut(s) 374
AluBI AGCT 1 cut(s) 279
AluI AGCT 1 cut(s) 279
AlwNI CAGNNNCTG 1 cut(s) 198
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 5 cut(s) 87, 97, 114, 126, 342
ApeKI GCWGC 2 cut(s) 17, 77
AspS9I GGNCC 2 cut(s) 97, 126
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 3 cut(s) 121, 172, 178
AvaI CYCGRG 1 cut(s) 284
BbvI GCAGC 2 cut(s) 29, 64
BceAI ACGGC 2 cut(s) 129, 198
BciT130I CCWGG 2 cut(s) 101, 134
BcnI CCSGG 1 cut(s) 373
BglI GCCNNNNNGGC 1 cut(s) 86
BisI GCNGC 6 cut(s) 18, 75, 78, 87, 342, 345
BlsI GCNGC 6 cut(s) 19, 76, 79, 88, 343, 346
Bme1390I CCNGG 3 cut(s) 101, 134, 373
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 2 cut(s) 97, 126
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 3 cut(s) 101, 134, 373
BmsI GCATC 3 cut(s) 29, 228, 312
BpuMI CCSGG 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 292
BsaJI CCNNGG 5 cut(s) 90, 117, 132, 231, 372
BsaWI WCCGGW 1 cut(s) 262
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 123, 351
Bse118I RCCGGY 1 cut(s) 312
BseBI CCWGG 2 cut(s) 101, 134
BseDI CCNNGG 5 cut(s) 90, 117, 132, 231, 372
BseGI GGATG 2 cut(s) 20, 259
BseLI CCNNNNNNNGG 3 cut(s) 106, 123, 351
BseX3I CGGCCG 1 cut(s) 342
BseXI GCAGC 2 cut(s) 29, 64
BseYI CCCAGC 1 cut(s) 122
BsgI GTGCAG 1 cut(s) 211
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 5 cut(s) 89, 99, 116, 128, 344
BsiEI CGRYCG 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 3 cut(s) 263, 313, 372
BslI CCNNNNNNNGG 3 cut(s) 106, 123, 351
BsnI GGCC 5 cut(s) 89, 99, 116, 128, 344
BsoBI CYCGRG 1 cut(s) 284
Bsp19I CCATGG 1 cut(s) 90
BspACI CCGC 9 cut(s) 68, 75, 86, 95, 185, 305, 335, 341, 345
BspANI GGCC 5 cut(s) 89, 99, 116, 128, 344
BspLI GGNNCC 1 cut(s) 127
BsrFI RCCGGY 1 cut(s) 312
BssAI RCCGGY 1 cut(s) 312
BssECI CCNNGG 5 cut(s) 90, 117, 132, 231, 372
BssNI GRCGYC 1 cut(s) 292
BssT1I CCWWGG 3 cut(s) 90, 117, 231
Bst2UI CCWGG 2 cut(s) 101, 134
Bst4CI ACNGT 2 cut(s) 35, 166
BstACI GRCGYC 1 cut(s) 292
BstC8I GCNNGC 4 cut(s) 72, 97, 281, 305
BstDSI CCRYGG 1 cut(s) 90
BstF5I GGATG 2 cut(s) 20, 259
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 8 cut(s) 83, 86, 92, 101, 276, 289, 309, 341
BstNI CCWGG 2 cut(s) 101, 134
BstSCI CCNGG 3 cut(s) 99, 132, 371
BstV1I GCAGC 2 cut(s) 29, 64
BstXI CCANNNNNNTGG 1 cut(s) 376
BstZI CGGCCG 1 cut(s) 342
BsuRI GGCC 5 cut(s) 89, 99, 116, 128, 344
BtgI CCRYGG 1 cut(s) 90
BtsCI GGATG 2 cut(s) 20, 259
Cac8I GCNNGC 4 cut(s) 72, 97, 281, 305
CaiI CAGNNNCTG 1 cut(s) 198
CciNI GCGGCCGC 1 cut(s) 342
Cfr10I RCCGGY 1 cut(s) 312
Cfr13I GGNCC 2 cut(s) 97, 126
CseI GACGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 31
CviAII CATG 2 cut(s) 91, 243
CviQI GTAC 1 cut(s) 31
EaeI YGGCCR 2 cut(s) 87, 342
EagI CGGCCG 1 cut(s) 342
EciI GGCGGA 2 cut(s) 200, 350
EclXI CGGCCG 1 cut(s) 342
Eco130I CCWWGG 3 cut(s) 90, 117, 231
Eco52I CGGCCG 1 cut(s) 342
Eco88I CYCGRG 1 cut(s) 284
EcoRII CCWGG 2 cut(s) 99, 132
EcoT14I CCWWGG 3 cut(s) 90, 117, 231
ErhI CCWWGG 3 cut(s) 90, 117, 231
FaeI CATG 2 cut(s) 94, 246
FaiI YATR 3 cut(s) 92, 244, 252
FatI CATG 2 cut(s) 90, 242
FauI CCCGC 3 cut(s) 75, 88, 312
Fnu4HI GCNGC 6 cut(s) 18, 75, 78, 87, 342, 345
FokI GGATG 2 cut(s) 7, 246
Fsp4HI GCNGC 6 cut(s) 18, 75, 78, 87, 342, 345
GluI GCNGC 6 cut(s) 18, 75, 78, 87, 342, 345
GsaI CCCAGC 1 cut(s) 126
HaeIII GGCC 5 cut(s) 89, 99, 116, 128, 344
HapII CCGG 3 cut(s) 263, 313, 372
HgaI GACGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 292
Hin1II CATG 2 cut(s) 94, 246
HinfI GANTC 2 cut(s) 137, 380
HpaII CCGG 3 cut(s) 263, 313, 372
HphI GGTGA 3 cut(s) 121, 172, 178
Hpy188III TCNNGA 1 cut(s) 286
HpyAV CCTTC 3 cut(s) 151, 199, 306
HpyCH4III ACNGT 2 cut(s) 35, 166
HpyCH4IV ACGT 1 cut(s) 29
HpyCH4V TGCA 2 cut(s) 192, 270
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 86, 92, 101, 276, 289, 309, 341
HpySE526I ACGT 1 cut(s) 29
Hsp92I GRCGYC 1 cut(s) 292
Hsp92II CATG 2 cut(s) 94, 246
Lsp1109I GCAGC 2 cut(s) 29, 64
LweI GCATC 3 cut(s) 29, 228, 312
MaeII ACGT 1 cut(s) 29
MaeIII GTNAC 4 cut(s) 109, 160, 166, 245
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 9 cut(s) 50, 55, 166, 208, 217, 282, 324, 331, 350
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 3 cut(s) 263, 313, 372
MspR9I CCNGG 3 cut(s) 101, 134, 373
MvaI CCWGG 2 cut(s) 101, 134
MwoI GCNNNNNNNGC 8 cut(s) 83, 86, 92, 101, 276, 289, 309, 341
NciI CCSGG 1 cut(s) 373
NcoI CCATGG 1 cut(s) 90
NlaIII CATG 2 cut(s) 94, 246
NlaIV GGNNCC 1 cut(s) 127
NmuCI GTSAC 3 cut(s) 109, 160, 166
NotI GCGGCCGC 1 cut(s) 342
OliI CACNNNNGTG 1 cut(s) 374
PaeR7I CTCGAG 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 326
PfeI GAWTC 1 cut(s) 380
PkrI GCNGC 6 cut(s) 19, 76, 79, 88, 343, 346
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
Psp6I CCWGG 2 cut(s) 99, 132
PspFI CCCAGC 1 cut(s) 122
PspGI CCWGG 2 cut(s) 99, 132
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 2 cut(s) 97, 126
PstNI CAGNNNCTG 1 cut(s) 198
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
RseI CAYNNNNRTG 1 cut(s) 374
SatI GCNGC 6 cut(s) 18, 75, 78, 87, 342, 345
Sau96I GGNCC 2 cut(s) 97, 126
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 3 cut(s) 101, 134, 373
SetI ASST 5 cut(s) 32, 162, 219, 257, 281
SfaNI GCATC 3 cut(s) 29, 228, 312
Sfr274I CTCGAG 1 cut(s) 284
SlaI CTCGAG 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
SsiI CCGC 9 cut(s) 68, 75, 86, 95, 185, 305, 335, 341, 345
StyD4I CCNGG 3 cut(s) 99, 132, 371
StyI CCWWGG 3 cut(s) 90, 117, 231
TaaI ACNGT 2 cut(s) 35, 166
TaiI ACGT 1 cut(s) 32
TaqI TCGA 5 cut(s) 12, 140, 285, 320, 383
TauI GCSGC 4 cut(s) 77, 89, 344, 347
TfiI GAWTC 1 cut(s) 380
TseFI GTSAC 3 cut(s) 109, 160, 166
TseI GCWGC 2 cut(s) 17, 77
Tsp45I GTSAC 3 cut(s) 109, 160, 166
TspDTI ATGAA 1 cut(s) 248
TspGWI ACGGA 1 cut(s) 185
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XhoI CTCGAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.