Rroxscaffold_2G00083210

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001784
Physical Location & Seq
Forward (+)
5889475 .. 5891472
1998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083210.1

Sequence Viewer

Length: 459 bp
ATGGTCATCATGTCCCAATCAATCTCTTCCGTCACACAAGAAGGGACGACGGAATCTGACGATCATTGCACTATTTGCTTGGAAGACTTGATTTCCAAGGTGAGGGAGATGCCTTGTAAGCATAGGTTTCACTCGCCTTGTATCGAGTCTTGGCTGCTCAGAAGTGAATCCTGCCCTCTTTGCAGGAGGCCGTTTCTGAGTGGCAGCAATCTCAGTCGTAGACTTTGGCGATTAGTCATCCGGCCATGGACAAAAAATATGAGCACCTACACCCATCTTCAAACACATGAACGGAACTCTCACTGCAAAAATGAGTCCCAAAGAACATGCCATCAGAAAATATATTCCTCCCACATACCTAGAAATCGGAACGCTTCCATGGATTCTCCAGAGTCCACACAAGACCCGGCCCCCAATTATTGTATTCCACATGACCAATTAAGCTCCTCGAACCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

152

Amino Acids

17.66

Weight (kDa)

8.63

Isoelectric Point (pI)

84.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 20 - 62 1.9e-07 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 20 - 65 2.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 22 - 62 5e-12 Ring finger domain
zf-C3HC4 PF00097 23 - 61 5.7e-11 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 23 - 61 7.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 23 - 48 4.6e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 220
AciI CCGC 1 cut(s) 454
AcoI YGGCCR 1 cut(s) 242
AfiI CCNNNNNNNGG 1 cut(s) 102
AgsI TTSAA 1 cut(s) 281
AluBI AGCT 1 cut(s) 444
AluI AGCT 1 cut(s) 444
Alw21I GWGCWC 1 cut(s) 266
AoxI GGCC 3 cut(s) 188, 242, 408
ApeKI GCWGC 2 cut(s) 154, 204
AspS9I GGNCC 1 cut(s) 409
AsuC2I CCSGG 1 cut(s) 407
AsuHPI GGTGA 1 cut(s) 112
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 266
BbvI GCAGC 2 cut(s) 141, 216
BccI CCATC 2 cut(s) 282, 339
BceAI ACGGC 1 cut(s) 175
BcnI CCSGG 1 cut(s) 407
BfaI CTAG 2 cut(s) 360, 457
BisI GCNGC 2 cut(s) 155, 205
BlsI GCNGC 2 cut(s) 156, 206
Bme1390I CCNGG 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 409
BmiI GGNNCC 1 cut(s) 411
BmrFI CCNGG 1 cut(s) 407
BmsI GCATC 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 372
BpuMI CCSGG 1 cut(s) 407
BsaJI CCNNGG 3 cut(s) 96, 245, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 64
BseDI CCNNGG 3 cut(s) 96, 245, 378
BseGI GGATG 1 cut(s) 237
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMI GCAATG 1 cut(s) 64
BseMII CTCAG 3 cut(s) 172, 188, 226
BseRI GAGGAG 1 cut(s) 436
BseXI GCAGC 2 cut(s) 141, 216
BshFI GGCC 3 cut(s) 190, 244, 410
BsiHKAI GWGCWC 1 cut(s) 266
BsiSI CCGG 2 cut(s) 241, 407
BslFI GGGAC 2 cut(s) 58, 301
BslI CCNNNNNNNGG 1 cut(s) 102
BsmFI GGGAC 2 cut(s) 58, 301
BsnI GGCC 3 cut(s) 190, 244, 410
Bsp1286I GDGCHC 1 cut(s) 266
Bsp143I GATC 1 cut(s) 61
Bsp19I CCATGG 2 cut(s) 245, 378
BspACI CCGC 1 cut(s) 454
BspANI GGCC 3 cut(s) 190, 244, 410
BspCNI CTCAG 3 cut(s) 171, 189, 225
BspLI GGNNCC 1 cut(s) 411
BsrDI GCAATG 1 cut(s) 64
BssECI CCNNGG 3 cut(s) 96, 245, 378
BssMI GATC 1 cut(s) 61
BssT1I CCWWGG 3 cut(s) 96, 245, 378
Bst6I CTCTTC 1 cut(s) 31
BstAPI GCANNNNNTGC 1 cut(s) 75
BstDEI CTNAG 3 cut(s) 158, 197, 212
BstDSI CCRYGG 2 cut(s) 245, 378
BstF5I GGATG 1 cut(s) 237
BstKTI GATC 1 cut(s) 64
BstMBI GATC 1 cut(s) 61
BstMWI GCNNNNNNNGC 3 cut(s) 75, 118, 180
BstNSI RCATGY 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 405
BstV1I GCAGC 2 cut(s) 141, 216
BstV2I GAAGAC 1 cut(s) 90
BsuRI GGCC 3 cut(s) 190, 244, 410
BtgI CCRYGG 2 cut(s) 245, 378
BtsCI GGATG 1 cut(s) 237
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 409
CviAII CATG 6 cut(s) 10, 246, 287, 327, 379, 431
CviJI RGCY 5 cut(s) 154, 190, 244, 410, 444
CviKI_1 RGCY 5 cut(s) 154, 190, 244, 410, 444
DdeI CTNAG 3 cut(s) 158, 197, 212
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 242
Eam1104I CTCTTC 1 cut(s) 31
EarI CTCTTC 1 cut(s) 31
Eco130I CCWWGG 3 cut(s) 96, 245, 378
EcoT14I CCWWGG 3 cut(s) 96, 245, 378
ErhI CCWWGG 3 cut(s) 96, 245, 378
FaeI CATG 6 cut(s) 13, 249, 290, 330, 382, 434
FaqI GGGAC 2 cut(s) 58, 301
FatI CATG 6 cut(s) 9, 245, 286, 326, 378, 430
FblI GTMKAC 1 cut(s) 220
Fnu4HI GCNGC 2 cut(s) 155, 205
FokI GGATG 1 cut(s) 224
Fsp4HI GCNGC 2 cut(s) 155, 205
FspBI CTAG 2 cut(s) 360, 457
GluI GCNGC 2 cut(s) 155, 205
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 3 cut(s) 190, 244, 410
HapII CCGG 2 cut(s) 241, 407
Hin1II CATG 6 cut(s) 13, 249, 290, 330, 382, 434
HinfI GANTC 6 cut(s) 53, 146, 167, 314, 383, 392
HpaII CCGG 2 cut(s) 241, 407
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 2 cut(s) 221, 396
Hpy188I TCNGA 5 cut(s) 58, 161, 198, 336, 369
Hpy188III TCNNGA 1 cut(s) 389
Hpy8I GTNNAC 2 cut(s) 221, 396
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 1 cut(s) 35
HpyCH4V TGCA 3 cut(s) 69, 183, 306
HpyF10VI GCNNNNNNNGC 3 cut(s) 75, 118, 180
HpyF3I CTNAG 3 cut(s) 158, 197, 212
Hsp92II CATG 6 cut(s) 13, 249, 290, 330, 382, 434
Kzo9I GATC 1 cut(s) 61
LmnI GCTCC 1 cut(s) 449
LpnPI CCDG 5 cut(s) 169, 184, 254, 402, 420
Lsp1109I GCAGC 2 cut(s) 141, 216
LweI GCATC 1 cut(s) 99
MaeI CTAG 2 cut(s) 360, 457
MaeIII GTNAC 1 cut(s) 31
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 3 cut(s) 18, 95, 269
MhlI GDGCHC 1 cut(s) 266
MluCI AATT 2 cut(s) 415, 437
MlyI GAGTC 3 cut(s) 155, 323, 401
MnlI CCTC 5 cut(s) 96, 180, 186, 358, 457
MseI TTAA 1 cut(s) 440
MspI CCGG 2 cut(s) 241, 407
MspR9I CCNGG 1 cut(s) 407
MwoI GCNNNNNNNGC 3 cut(s) 75, 118, 180
NciI CCSGG 1 cut(s) 407
NcoI CCATGG 2 cut(s) 245, 378
NdeII GATC 1 cut(s) 61
NlaIII CATG 6 cut(s) 13, 249, 290, 330, 382, 434
NlaIV GGNNCC 1 cut(s) 411
NmuCI GTSAC 1 cut(s) 31
NspI RCATGY 1 cut(s) 330
PfeI GAWTC 3 cut(s) 53, 167, 383
PkrI GCNGC 2 cut(s) 156, 206
PleI GAGTC 3 cut(s) 154, 322, 400
PpsI GAGTC 3 cut(s) 154, 322, 400
PspN4I GGNNCC 1 cut(s) 411
PspPI GGNCC 1 cut(s) 409
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 2 cut(s) 155, 205
Sau3AI GATC 1 cut(s) 61
Sau96I GGNCC 1 cut(s) 409
SchI GAGTC 3 cut(s) 155, 323, 401
ScrFI CCNGG 1 cut(s) 407
SduI GDGCHC 1 cut(s) 266
SetI ASST 5 cut(s) 102, 128, 269, 361, 446
SfaNI GCATC 1 cut(s) 99
Sse9I AATT 2 cut(s) 415, 437
SsiI CCGC 1 cut(s) 454
SspMI CTAG 2 cut(s) 360, 457
StyD4I CCNGG 1 cut(s) 405
StyI CCWWGG 3 cut(s) 96, 245, 378
TaqI TCGA 2 cut(s) 144, 449
TasI AATT 2 cut(s) 415, 437
TfiI GAWTC 3 cut(s) 53, 167, 383
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TscAI CASTG 1 cut(s) 308
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 2 cut(s) 154, 204
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 1 cut(s) 303
TspGWI ACGGA 3 cut(s) 19, 65, 307
TspRI CASTG 1 cut(s) 308
XceI RCATGY 1 cut(s) 330
XmiI GTMKAC 1 cut(s) 220
XspI CTAG 2 cut(s) 360, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.