RLG00000021487

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001784
Physical Location & Seq
Forward (+)
75819815 .. 75821006
1192 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021487

Sequence Viewer

Length: 432 bp
ATGTCCCAATCAATCTCTTCCGTCACAGAAGAAGGGACGACGGAATATGATGATCATTGCACTATTTGCTTGGAAGACTTGATTTCCAAGGTGAGGGAGATGCCTTGTAAGCATAGGTTTCACTCGCCTTGTATCGAGTCTTGGCTGCTCAGAAGTGAATCCTGCCCTCTTTGCAGGAGGTCGTTTCTGAGTGGCAGCAATCTCATTCGTAGACTTTGGCGATTAGCATATGCATATTTTGCTAATGTTTTCATTGTTTTTTGTTGGATTACAGTTGTCATTGTCTTCCTTGTGACCATTCACCCATGTACTGATGATCACAATGGCGATCAGGTTTCTGGCAGCGAAAACGGCGTCGTGGTGCGCACTGAGATGGACACGCTAAACATAAGCTTCCACTGTTTACTGTTACAGAGGAAGCTTCCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

144

Amino Acids

16.44

Weight (kDa)

6.04

Isoelectric Point (pI)

74.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 15 - 59 8.1e-08 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 17 - 61 6.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 19 - 59 4.4e-12 Ring finger domain
zf-C3HC4 PF00097 20 - 58 5.1e-11 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 20 - 58 6.3e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 20 - 45 4.2e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 365
AccI GTMKAC 1 cut(s) 211
AcyI GRCGYC 1 cut(s) 354
AfaI GTAC 1 cut(s) 310
AfiI CCNNNNNNNGG 1 cut(s) 93
AluBI AGCT 2 cut(s) 393, 421
AluI AGCT 2 cut(s) 393, 421
ApeKI GCWGC 3 cut(s) 145, 195, 342
ArsI GACNNNNNNTTYG 2 cut(s) 339, 371
AspLEI GCGC 1 cut(s) 366
AsuHPI GGTGA 2 cut(s) 103, 293
BbsI GAAGAC 2 cut(s) 81, 277
BbvI GCAGC 3 cut(s) 132, 207, 354
BccI CCATC 1 cut(s) 367
BceAI ACGGC 1 cut(s) 367
BclI TGATCA 2 cut(s) 52, 316
BisI GCNGC 3 cut(s) 146, 196, 343
BlsI GCNGC 3 cut(s) 147, 197, 344
BmsI GCATC 1 cut(s) 90
BpiI GAAGAC 2 cut(s) 81, 277
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 1 cut(s) 87
Bsc4I CCNNNNNNNGG 1 cut(s) 93
Bse3DI GCAATG 1 cut(s) 55
BseDI CCNNGG 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 93
BseMI GCAATG 1 cut(s) 55
BseMII CTCAG 3 cut(s) 163, 179, 360
BseXI GCAGC 3 cut(s) 132, 207, 354
BslFI GGGAC 1 cut(s) 49
BslI CCNNNNNNNGG 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 49
Bsp143I GATC 3 cut(s) 52, 316, 328
BspCNI CTCAG 3 cut(s) 162, 180, 361
BsrDI GCAATG 1 cut(s) 55
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 3 cut(s) 52, 316, 328
BssNI GRCGYC 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 87
Bst4CI ACNGT 3 cut(s) 274, 401, 408
Bst6I CTCTTC 1 cut(s) 22
BstACI GRCGYC 1 cut(s) 354
BstAPI GCANNNNNTGC 2 cut(s) 66, 239
BstDEI CTNAG 3 cut(s) 149, 188, 369
BstHHI GCGC 1 cut(s) 366
BstKTI GATC 3 cut(s) 55, 319, 331
BstMBI GATC 3 cut(s) 52, 316, 328
BstMWI GCNNNNNNNGC 5 cut(s) 66, 109, 171, 239, 351
BstV1I GCAGC 3 cut(s) 132, 207, 354
BstV2I GAAGAC 2 cut(s) 81, 277
BtsIMutI CAGTG 2 cut(s) 366, 397
CfoI GCGC 1 cut(s) 366
CseI GACGC 1 cut(s) 343
Csp6I GTAC 1 cut(s) 309
CviAII CATG 1 cut(s) 306
CviJI RGCY 3 cut(s) 145, 393, 421
CviKI_1 RGCY 3 cut(s) 145, 393, 421
CviQI GTAC 1 cut(s) 309
DdeI CTNAG 3 cut(s) 149, 188, 369
DpnI GATC 3 cut(s) 54, 318, 330
DpnII GATC 3 cut(s) 52, 316, 328
Eam1104I CTCTTC 1 cut(s) 22
EarI CTCTTC 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 87
EcoT22I ATGCAT 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 1 cut(s) 309
FaiI YATR 7 cut(s) 48, 114, 229, 231, 235, 307, 389
FaqI GGGAC 1 cut(s) 49
FatI CATG 1 cut(s) 305
FauNDI CATATG 1 cut(s) 229
FbaI TGATCA 2 cut(s) 52, 316
FblI GTMKAC 1 cut(s) 211
Fnu4HI GCNGC 3 cut(s) 146, 196, 343
Fsp4HI GCNGC 3 cut(s) 146, 196, 343
FspAI RTGCGCAY 1 cut(s) 365
FspI TGCGCA 1 cut(s) 365
GlaI GCGC 1 cut(s) 365
GluI GCNGC 3 cut(s) 146, 196, 343
HgaI GACGC 1 cut(s) 343
HhaI GCGC 1 cut(s) 366
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 1 cut(s) 309
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HindIII AAGCTT 2 cut(s) 391, 419
HinfI GANTC 2 cut(s) 137, 158
HphI GGTGA 2 cut(s) 103, 293
Hpy166II GTNNAC 2 cut(s) 212, 404
Hpy188I TCNGA 2 cut(s) 152, 189
Hpy8I GTNNAC 2 cut(s) 212, 404
Hpy99I CGWCG 2 cut(s) 43, 359
HpyAV CCTTC 1 cut(s) 26
HpyCH4III ACNGT 3 cut(s) 274, 401, 408
HpyCH4V TGCA 3 cut(s) 60, 174, 233
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 109, 171, 239, 351
HpyF3I CTNAG 3 cut(s) 149, 188, 369
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 1 cut(s) 309
HspAI GCGC 1 cut(s) 364
Ksp22I TGATCA 2 cut(s) 52, 316
Kzo9I GATC 3 cut(s) 52, 316, 328
LpnPI CCDG 4 cut(s) 160, 175, 317, 324
Lsp1109I GCAGC 3 cut(s) 132, 207, 354
LweI GCATC 1 cut(s) 90
MaeIII GTNAC 3 cut(s) 22, 292, 408
MalI GATC 3 cut(s) 54, 318, 330
MboI GATC 3 cut(s) 52, 316, 328
MboII GAAGA 4 cut(s) 9, 41, 86, 277
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 4 cut(s) 87, 171, 177, 408
Mph1103I ATGCAT 1 cut(s) 235
MslI CAYNNNNRTG 1 cut(s) 371
MwoI GCNNNNNNNGC 5 cut(s) 66, 109, 171, 239, 351
NdeI CATATG 1 cut(s) 229
NdeII GATC 3 cut(s) 52, 316, 328
NlaIII CATG 1 cut(s) 309
NmuCI GTSAC 2 cut(s) 22, 292
NsbI TGCGCA 1 cut(s) 365
NsiI ATGCAT 1 cut(s) 235
PfeI GAWTC 1 cut(s) 158
PkrI GCNGC 3 cut(s) 147, 197, 344
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
RsaI GTAC 1 cut(s) 310
RsaNI GTAC 1 cut(s) 309
RseI CAYNNNNRTG 1 cut(s) 371
SatI GCNGC 3 cut(s) 146, 196, 343
Sau3AI GATC 3 cut(s) 52, 316, 328
SchI GAGTC 1 cut(s) 146
SetI ASST 6 cut(s) 93, 119, 182, 336, 395, 423
SfaNI GCATC 1 cut(s) 90
SmiMI CAYNNNNRTG 1 cut(s) 371
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 3 cut(s) 274, 401, 408
TaqI TCGA 1 cut(s) 135
TatI WGTACW 1 cut(s) 308
TfiI GAWTC 1 cut(s) 158
TscAI CASTG 2 cut(s) 373, 404
TseFI GTSAC 2 cut(s) 22, 292
TseI GCWGC 3 cut(s) 145, 195, 342
Tsp45I GTSAC 2 cut(s) 22, 292
TspDTI ATGAA 1 cut(s) 241
TspGWI ACGGA 2 cut(s) 10, 56
TspRI CASTG 2 cut(s) 373, 404
XmiI GTMKAC 1 cut(s) 211
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.