Rh4BG015000

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001784
Physical Location & Seq
Reverse (-)
2134532 .. 2135119
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG015000.1

Sequence Viewer

Length: 588 bp
ATGTCTAATTCCGAAGCAAACGACGATGGTCAATTGTCCCTATCGGAATCATTAGTCTCTTTCTCGGACCAACGCGTGCCAATATTGCACAGTTTTACTATCAGTGGGCCAATATTTACTGGAGGGCTACCTGGTGTCATCGATCGGACATCCTCTGTGTCGCGGCCGAGGGCATCCGAGGTCTCCATAGAGGCCATGCCAACTGTGACAGTGGCATCTGAGGAAGGTAATGAATGCAGTATCTGCTTGGATGGCTTGAAGGTCGGCGACGAAGCCAAGGAAATGCCTTGCAAGCACAGGTTCCACAGGGCCTGTATCAAAAAGTGGATGGGGATGAGCACTCTCTGCCCGCTTTGCAGGTTCTCTTTGCCGGCAGAGTCAGAAGAGGAAGAAAATAGTGATGATGGTGACGATCATTTTAGCCTTTTTAGGTCTATTCTTGATGTTGTTAACAATGGCAGTGATACTGAAGATGACTCTGAAAGTGAGGAAGATATCGGAGGTGAATGGGAATCAAGCACTGTCGATGATGATGATGCTTCAGTCGGAGATAATGTCACCTTCAGGTTTAATTTTAGTATTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

195

Amino Acids

21.27

Weight (kDa)

4.18

Isoelectric Point (pI)

58.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 77 - 120 3.6e-15 Ring finger domain
zf-RING_11 PF17123 78 - 106 2.5e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 78 - 119 9.1e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 79 - 119 8.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 96 - 120 1.9e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 348
AccII CGCG 2 cut(s) 75, 163
AciI CCGC 2 cut(s) 163, 350
AcoI YGGCCR 1 cut(s) 164
AcuI CTGAAG 3 cut(s) 489, 525, 547
AflIII ACRYGT 1 cut(s) 73
AgsI TTSAA 2 cut(s) 259, 584
AjnI CCWGG 1 cut(s) 130
Alw21I GWGCWC 1 cut(s) 341
Alw26I GTCTC 2 cut(s) 61, 187
AlwNI CAGNNNCTG 2 cut(s) 243, 312
AoxI GGCC 4 cut(s) 107, 164, 192, 309
AspS9I GGNCC 3 cut(s) 67, 107, 309
AsuHPI GGTGA 3 cut(s) 419, 515, 550
AvaII GGWCC 1 cut(s) 67
Bbv12I GWGCWC 1 cut(s) 341
BccI CCATC 4 cut(s) 20, 245, 322, 398
BciT130I CCWGG 1 cut(s) 132
BcoDI GTCTC 2 cut(s) 61, 187
BfuAI ACCTGC 1 cut(s) 348
BisI GCNGC 1 cut(s) 164
BlsI GCNGC 1 cut(s) 165
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 1 cut(s) 67
BmgT120I GGNCC 3 cut(s) 67, 107, 309
BmiI GGNNCC 1 cut(s) 302
BmrFI CCNGG 1 cut(s) 132
BmsI GCATC 3 cut(s) 182, 224, 526
BoxI GACNNNNGTC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 141
Bsa29I ATCGAT 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 3 cut(s) 167, 177, 276
BsaXI ACNNNNNCTCC 2 cut(s) 540, 570
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 1 cut(s) 124
BseBI CCWGG 1 cut(s) 132
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 3 cut(s) 167, 177, 276
BseGI GGATG 5 cut(s) 149, 173, 256, 333, 339
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 124
BseX3I CGGCCG 1 cut(s) 164
Bsh1236I CGCG 2 cut(s) 75, 163
Bsh1285I CGRYCG 2 cut(s) 145, 167
BshFI GGCC 4 cut(s) 109, 166, 194, 311
BshVI ATCGAT 1 cut(s) 141
BsiEI CGRYCG 2 cut(s) 145, 167
BsiHKAI GWGCWC 1 cut(s) 341
BsiSI CCGG 1 cut(s) 371
BslFI GGGAC 1 cut(s) 22
BsmAI GTCTC 2 cut(s) 61, 187
BsmFI GGGAC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 239
BsnI GGCC 4 cut(s) 109, 166, 194, 311
Bso31I GGTCTC 1 cut(s) 187
Bsp1286I GDGCHC 1 cut(s) 341
Bsp143I GATC 2 cut(s) 142, 412
BspACI CCGC 2 cut(s) 163, 350
BspANI GGCC 4 cut(s) 109, 166, 194, 311
BspCNI CTCAG 1 cut(s) 211
BspDI ATCGAT 1 cut(s) 141
BspFNI CGCG 2 cut(s) 75, 163
BspLI GGNNCC 1 cut(s) 302
BspMI ACCTGC 1 cut(s) 348
BspTNI GGTCTC 1 cut(s) 187
BsrFI RCCGGY 1 cut(s) 370
BsrI ACTGG 1 cut(s) 124
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 3 cut(s) 167, 177, 276
BssMI GATC 2 cut(s) 142, 412
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 4 cut(s) 92, 205, 211, 523
Bst6I CTCTTC 1 cut(s) 378
BstAPI GCANNNNNTGC 2 cut(s) 243, 345
BstC8I GCNNGC 4 cut(s) 77, 293, 350, 372
BstDEI CTNAG 1 cut(s) 219
BstF5I GGATG 5 cut(s) 149, 173, 256, 333, 339
BstFNI CGCG 2 cut(s) 75, 163
BstKTI GATC 2 cut(s) 145, 415
BstMAI GTCTC 2 cut(s) 61, 187
BstMBI GATC 2 cut(s) 142, 412
BstMCI CGRYCG 2 cut(s) 145, 167
BstMWI GCNNNNNNNGC 6 cut(s) 85, 243, 252, 292, 345, 354
BstNI CCWGG 1 cut(s) 132
BstPAI GACNNNNGTC 1 cut(s) 27
BstSCI CCNGG 1 cut(s) 130
BstUI CGCG 2 cut(s) 75, 163
BstZI CGGCCG 1 cut(s) 164
Bsu15I ATCGAT 1 cut(s) 141
BsuRI GGCC 4 cut(s) 109, 166, 194, 311
BsuTUI ATCGAT 1 cut(s) 141
BtsCI GGATG 5 cut(s) 149, 173, 256, 333, 339
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 4 cut(s) 109, 216, 466, 519
BveI ACCTGC 1 cut(s) 348
Cac8I GCNNGC 4 cut(s) 77, 293, 350, 372
CaiI CAGNNNCTG 2 cut(s) 243, 312
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 3 cut(s) 67, 107, 309
ClaI ATCGAT 1 cut(s) 141
CsiI ACCWGGT 1 cut(s) 130
CviAII CATG 1 cut(s) 196
CviJI RGCY 8 cut(s) 109, 127, 166, 194, 255, 275, 311, 423
CviKI_1 RGCY 8 cut(s) 109, 127, 166, 194, 255, 275, 311, 423
DdeI CTNAG 1 cut(s) 219
DpnI GATC 2 cut(s) 144, 414
DpnII GATC 2 cut(s) 142, 412
EaeI YGGCCR 1 cut(s) 164
EagI CGGCCG 1 cut(s) 164
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
EclXI CGGCCG 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 276
Eco31I GGTCTC 1 cut(s) 187
Eco32I GATATC 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 67
Eco52I CGGCCG 1 cut(s) 164
Eco57I CTGAAG 3 cut(s) 489, 525, 547
EcoO109I RGGNCCY 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 130
EcoRV GATATC 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 1 cut(s) 199
FaiI YATR 2 cut(s) 188, 197
FaqI GGGAC 1 cut(s) 22
FatI CATG 1 cut(s) 195
FauI CCCGC 1 cut(s) 357
Fnu4HI GCNGC 1 cut(s) 164
FokI GGATG 5 cut(s) 136, 160, 263, 340, 346
Fsp4HI GCNGC 1 cut(s) 164
GluI GCNGC 1 cut(s) 164
GsuI CTGGAG 1 cut(s) 141
HaeIII GGCC 4 cut(s) 109, 166, 194, 311
HapII CCGG 1 cut(s) 371
Hin1II CATG 1 cut(s) 199
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HinfI GANTC 4 cut(s) 47, 377, 476, 512
HpaI GTTAAC 1 cut(s) 451
HpaII CCGG 1 cut(s) 371
HphI GGTGA 3 cut(s) 419, 515, 550
Hpy166II GTNNAC 1 cut(s) 451
Hpy188III TCNNGA 1 cut(s) 440
Hpy8I GTNNAC 1 cut(s) 451
Hpy99I CGWCG 2 cut(s) 26, 272
HpyAV CCTTC 3 cut(s) 218, 253, 571
HpyCH4III ACNGT 4 cut(s) 92, 205, 211, 523
HpyCH4V TGCA 4 cut(s) 88, 237, 291, 357
HpyF10VI GCNNNNNNNGC 6 cut(s) 85, 243, 252, 292, 345, 354
HpyF3I CTNAG 1 cut(s) 219
Hsp92II CATG 1 cut(s) 199
KroI GCCGGC 1 cut(s) 370
KroNI GCCGGC 1 cut(s) 372
KspAI GTTAAC 1 cut(s) 451
Kzo9I GATC 2 cut(s) 142, 412
LpnPI CCDG 9 cut(s) 105, 117, 144, 283, 292, 325, 343, 384, 550
LweI GCATC 3 cut(s) 182, 224, 526
MabI ACCWGGT 1 cut(s) 130
MaeIII GTNAC 3 cut(s) 205, 407, 556
MalI GATC 2 cut(s) 144, 414
MboI GATC 2 cut(s) 142, 412
MboII GAAGA 4 cut(s) 395, 401, 482, 503
MfeI CAATTG 1 cut(s) 32
MhlI GDGCHC 1 cut(s) 341
MluCI AATT 3 cut(s) 7, 32, 571
MluI ACGCGT 1 cut(s) 73
MlyI GAGTC 2 cut(s) 386, 470
MmeI TCCRAC 1 cut(s) 526
MnlI CCTC 9 cut(s) 116, 162, 163, 172, 184, 214, 379, 481, 494
MroNI GCCGGC 1 cut(s) 370
MseI TTAA 2 cut(s) 450, 570
MspI CCGG 1 cut(s) 371
MspR9I CCNGG 1 cut(s) 132
MunI CAATTG 1 cut(s) 32
Mva1269I GAATGC 1 cut(s) 239
MvaI CCWGG 1 cut(s) 132
MvnI CGCG 2 cut(s) 75, 163
MwoI GCNNNNNNNGC 6 cut(s) 85, 243, 252, 292, 345, 354
NaeI GCCGGC 1 cut(s) 372
NdeII GATC 2 cut(s) 142, 412
NgoMIV GCCGGC 1 cut(s) 370
NlaIII CATG 1 cut(s) 199
NlaIV GGNNCC 1 cut(s) 302
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 3 cut(s) 205, 407, 556
PctI GAATGC 1 cut(s) 239
PdiI GCCGGC 1 cut(s) 372
PfeI GAWTC 2 cut(s) 47, 512
PkrI GCNGC 1 cut(s) 165
Ple19I CGATCG 1 cut(s) 145
PleI GAGTC 2 cut(s) 385, 470
PpsI GAGTC 2 cut(s) 385, 470
PshAI GACNNNNGTC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 1 cut(s) 302
PspPI GGNCC 3 cut(s) 67, 107, 309
PstNI CAGNNNCTG 2 cut(s) 243, 312
PvuI CGATCG 1 cut(s) 145
SaqAI TTAA 2 cut(s) 450, 570
SatI GCNGC 1 cut(s) 164
Sau3AI GATC 2 cut(s) 142, 412
Sau96I GGNCC 3 cut(s) 67, 107, 309
SchI GAGTC 2 cut(s) 386, 470
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 1 cut(s) 341
SexAI ACCWGGT 1 cut(s) 130
SfaNI GCATC 3 cut(s) 182, 224, 526
SinI GGWCC 1 cut(s) 67
Sse9I AATT 3 cut(s) 7, 32, 571
SsiI CCGC 2 cut(s) 163, 350
SspI AATATT 2 cut(s) 84, 114
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 4 cut(s) 92, 205, 211, 523
TaqI TCGA 2 cut(s) 141, 525
TasI AATT 3 cut(s) 7, 32, 571
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 2 cut(s) 47, 512
Tru1I TTAA 2 cut(s) 450, 570
Tru9I TTAA 2 cut(s) 450, 570
TscAI CASTG 4 cut(s) 109, 216, 466, 526
TseFI GTSAC 3 cut(s) 205, 407, 556
Tsp45I GTSAC 3 cut(s) 205, 407, 556
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 4 cut(s) 109, 216, 466, 526
VpaK11BI GGWCC 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.