Rroxscaffold_2G00083200

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001784
Physical Location & Seq
Forward (+)
5888611 .. 5889051
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083200.1

Sequence Viewer

Length: 441 bp
ATGTCTGACTTTTCCAACCCACGTACTAGAGATCAAATAATAGCGGAAACCCCCGTACTCGCCTTACCTACACCAGCCACTGGCGTCAGGGCGTCAGGGCAACAATTACTTGCTTATATTGATTTTGTATTAGCTGACCAAACAACCGTGGCGGTGGGGGCATCAAAGGCATCCATAGAAGACATGCCGAGAGTGACAGTCACCGAGGAAGGAACGGAGTGCAGTATATGCTTGGAAAGCTTCAAAGTCTGCGCTGAAGCCAAAGAGATGCCTTGTAAGCACAGGTTTCACTCGCACTGTATTGAAAACTGGCTGTACAGGCGTGCTTCCTGCCCACTTTGCAGGTTCACCATGCCCGTTGACTTTGAAGAAGAGATGAACGGCATGAGCGAACATTGGATTGATATCACGGATTTTTTGGATTTTAGGGATATGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

146

Amino Acids

16.51

Weight (kDa)

4.69

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 73 - 115 5e-13 Ring finger domain
zf-RING_11 PF17123 73 - 101 8.7e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 73 - 114 5.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 74 - 115 5.3e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 74 - 114 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 74 - 112 2.6e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 333
AccB7I CCANNNNNTGG 1 cut(s) 80
AciI CCGC 2 cut(s) 44, 152
AcuI CTGAAG 1 cut(s) 276
AcyI GRCGYC 2 cut(s) 84, 92
AfaI GTAC 3 cut(s) 25, 57, 317
AfiI CCNNNNNNNGG 1 cut(s) 80
AgsI TTSAA 3 cut(s) 244, 305, 368
AluBI AGCT 2 cut(s) 134, 240
AluI AGCT 2 cut(s) 134, 240
AlwNI CAGNNNCTG 1 cut(s) 80
AspLEI GCGC 1 cut(s) 254
AsuHPI GGTGA 2 cut(s) 193, 340
BbsI GAAGAC 1 cut(s) 186
BceAI ACGGC 1 cut(s) 397
BfaI CTAG 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 333
BmsI GCATC 3 cut(s) 170, 179, 258
BpiI GAAGAC 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 23
BsaBI GATNNNNATC 1 cut(s) 404
BsaHI GRCGYC 2 cut(s) 84, 92
BsaJI CCNNGG 2 cut(s) 147, 204
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse1I ACTGG 2 cut(s) 85, 314
Bse8I GATNNNNATC 1 cut(s) 404
BseDI CCNNGG 2 cut(s) 147, 204
BseGI GGATG 1 cut(s) 170
BseJI GATNNNNATC 1 cut(s) 404
BseLI CCNNNNNNNGG 1 cut(s) 80
BseNI ACTGG 2 cut(s) 85, 314
BsgI GTGCAG 1 cut(s) 241
BslI CCNNNNNNNGG 1 cut(s) 80
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 1 cut(s) 31
BspACI CCGC 2 cut(s) 44, 152
BspMI ACCTGC 1 cut(s) 333
BsrGI TGTACA 1 cut(s) 315
BsrI ACTGG 2 cut(s) 85, 314
BssECI CCNNGG 2 cut(s) 147, 204
BssMI GATC 1 cut(s) 31
BssNI GRCGYC 2 cut(s) 84, 92
Bst4CI ACNGT 3 cut(s) 148, 199, 299
Bst6I CTCTTC 1 cut(s) 366
BstACI GRCGYC 2 cut(s) 84, 92
BstAPI GCANNNNNTGC 1 cut(s) 228
BstAUI TGTACA 1 cut(s) 315
BstBAI YACGTR 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 1 cut(s) 170
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 1 cut(s) 34
BstMBI GATC 1 cut(s) 31
BstMWI GCNNNNNNNGC 7 cut(s) 158, 167, 228, 237, 277, 319, 339
BstNSI RCATGY 1 cut(s) 187
BstV2I GAAGAC 1 cut(s) 186
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 1 cut(s) 170
BtsIMutI CAGTG 2 cut(s) 78, 295
BveI ACCTGC 1 cut(s) 333
Cac8I GCNNGC 1 cut(s) 324
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 1 cut(s) 254
CseI GACGC 2 cut(s) 73, 81
Csp6I GTAC 3 cut(s) 24, 56, 316
CviAII CATG 3 cut(s) 184, 352, 385
CviJI RGCY 5 cut(s) 77, 134, 240, 260, 313
CviKI_1 RGCY 5 cut(s) 77, 134, 240, 260, 313
CviQI GTAC 3 cut(s) 24, 56, 316
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Eco32I GATATC 1 cut(s) 406
Eco57I CTGAAG 1 cut(s) 276
EcoRV GATATC 1 cut(s) 406
FaeI CATG 3 cut(s) 187, 355, 388
FaiI YATR 8 cut(s) 117, 176, 185, 227, 229, 353, 386, 434
FatI CATG 3 cut(s) 183, 351, 384
FokI GGATG 1 cut(s) 157
FspBI CTAG 1 cut(s) 27
GlaI GCGC 1 cut(s) 253
HgaI GACGC 2 cut(s) 73, 81
HhaI GCGC 1 cut(s) 254
Hin1I GRCGYC 2 cut(s) 84, 92
Hin1II CATG 3 cut(s) 187, 355, 388
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 238
HphI GGTGA 2 cut(s) 193, 340
Hpy166II GTNNAC 2 cut(s) 348, 361
Hpy188I TCNGA 1 cut(s) 7
Hpy8I GTNNAC 2 cut(s) 348, 361
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 3 cut(s) 148, 199, 299
HpyCH4IV ACGT 1 cut(s) 22
HpyCH4V TGCA 2 cut(s) 222, 342
HpyF10VI GCNNNNNNNGC 7 cut(s) 158, 167, 228, 237, 277, 319, 339
HpySE526I ACGT 1 cut(s) 22
Hsp92I GRCGYC 2 cut(s) 84, 92
Hsp92II CATG 3 cut(s) 187, 355, 388
HspAI GCGC 1 cut(s) 252
Kzo9I GATC 1 cut(s) 31
LpnPI CCDG 9 cut(s) 66, 73, 81, 87, 268, 295, 304, 328, 343
LweI GCATC 3 cut(s) 170, 179, 258
MaeI CTAG 1 cut(s) 27
MaeII ACGT 1 cut(s) 22
MaeIII GTNAC 2 cut(s) 193, 199
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MboII GAAGA 3 cut(s) 191, 380, 383
MluCI AATT 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 1 cut(s) 199
MwoI GCNNNNNNNGC 7 cut(s) 158, 167, 228, 237, 277, 319, 339
NdeII GATC 1 cut(s) 31
NlaIII CATG 3 cut(s) 187, 355, 388
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 2 cut(s) 193, 199
NspI RCATGY 1 cut(s) 187
PcsI WCGNNNNNNNCGW 1 cut(s) 387
PflMI CCANNNNNTGG 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 23
PstNI CAGNNNCTG 1 cut(s) 80
RsaI GTAC 3 cut(s) 25, 57, 317
RsaNI GTAC 3 cut(s) 24, 56, 316
Sau3AI GATC 1 cut(s) 31
SetI ASST 6 cut(s) 25, 70, 136, 242, 287, 347
SfaNI GCATC 3 cut(s) 170, 179, 258
Sse9I AATT 1 cut(s) 104
SsiI CCGC 2 cut(s) 44, 152
SspMI CTAG 1 cut(s) 27
TaaI ACNGT 3 cut(s) 148, 199, 299
TaiI ACGT 1 cut(s) 25
TasI AATT 1 cut(s) 104
TatI WGTACW 1 cut(s) 315
TscAI CASTG 2 cut(s) 85, 302
TseFI GTSAC 2 cut(s) 193, 199
Tsp45I GTSAC 2 cut(s) 193, 199
TspDTI ATGAA 1 cut(s) 392
TspGWI ACGGA 2 cut(s) 230, 425
TspRI CASTG 2 cut(s) 85, 302
Van91I CCANNNNNTGG 1 cut(s) 80
XceI RCATGY 1 cut(s) 187
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.