RchiOBHm_Chr2g0168291

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001784
Physical Location & Seq
Reverse (-)
82695559 .. 82696059
501 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53597

Sequence Viewer

Length: 501 bp
ATGGTCAAAATGTCCCAGTCAATCTCTTCCGTCACAGAAGAAGGGACGACGGAATATGACGATCATTGCACTATTTGCTTGGAAGACTTGATTTCCAAGGTGAGGGAGATGCCTTGTAAGCATAGGTTTCACTCCCCTTGTATCGAGTCTTGGCTGCTCAGAAGTGAATCCTGCCCTCTTTGCAGGAGGCCGTTTCTGAGTGGCAGCAATCTGAGTCGTAGACTTTGGCGATTAGTATATGCATATTTTGCTAATGTTTTAATTATTTTTTGTTGGATTACAGGTTGGTTGTGTGCATGGTTGATTTATTTGAATCTAGCTATTTGCCCAGTAATATCCTCTGTTTGCCCTCTTTACCCCTTTGAAAAAGCGACTTGGATCATCTCCTCCTTGTTTTTAGTTGTCATTGTCTTCCTTGTGACCATTCACCCATGTCCTGATGATCGCCGTGGCGATCAGGTGATCGATGAACTTGAAGGAAACTTGAGAACCCAGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

166

Amino Acids

19.18

Weight (kDa)

6.06

Isoelectric Point (pI)

73.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 18 - 62 7.6e-08 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 20 - 65 4.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 22 - 62 5.9e-12 Ring finger domain
zf-C3HC4 PF00097 23 - 61 6.7e-11 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 23 - 61 8.3e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 23 - 48 5.3e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 220
AclWI GGATC 1 cut(s) 386
AfiI CCNNNNNNNGG 1 cut(s) 102
AgsI TTSAA 3 cut(s) 313, 365, 476
AluBI AGCT 1 cut(s) 320
AluI AGCT 1 cut(s) 320
AlwI GGATC 1 cut(s) 386
AoxI GGCC 1 cut(s) 188
ApeKI GCWGC 2 cut(s) 154, 204
AsuHPI GGTGA 3 cut(s) 112, 419, 472
BbsI GAAGAC 2 cut(s) 90, 403
BbvI GCAGC 2 cut(s) 141, 216
BceAI ACGGC 2 cut(s) 175, 432
BfaI CTAG 1 cut(s) 317
BisI GCNGC 2 cut(s) 155, 205
BlsI GCNGC 2 cut(s) 156, 206
BmrI ACTGGG 3 cut(s) 10, 323, 487
BmsI GCATC 1 cut(s) 99
BmuI ACTGGG 3 cut(s) 10, 323, 487
BpiI GAAGAC 2 cut(s) 90, 403
Bsa29I ATCGAT 1 cut(s) 465
BsaJI CCNNGG 2 cut(s) 96, 448
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse1I ACTGG 3 cut(s) 16, 329, 493
Bse3DI GCAATG 1 cut(s) 64
BseCI ATCGAT 1 cut(s) 465
BseDI CCNNGG 2 cut(s) 96, 448
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMI GCAATG 1 cut(s) 64
BseMII CTCAG 3 cut(s) 172, 188, 203
BseNI ACTGG 3 cut(s) 16, 329, 493
BseRI GAGGAG 1 cut(s) 376
BseXI GCAGC 2 cut(s) 141, 216
BshFI GGCC 1 cut(s) 190
BshVI ATCGAT 1 cut(s) 465
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 1 cut(s) 190
Bsp143I GATC 5 cut(s) 61, 378, 442, 454, 462
BspANI GGCC 1 cut(s) 190
BspCNI CTCAG 3 cut(s) 171, 189, 204
BspDI ATCGAT 1 cut(s) 465
BspHI TCATGA 1 cut(s) 497
BspPI GGATC 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 64
BsrI ACTGG 3 cut(s) 16, 329, 493
BssECI CCNNGG 2 cut(s) 96, 448
BssMI GATC 5 cut(s) 61, 378, 442, 454, 462
BssT1I CCWWGG 1 cut(s) 96
Bst6I CTCTTC 1 cut(s) 31
BstAPI GCANNNNNTGC 2 cut(s) 75, 248
BstDEI CTNAG 3 cut(s) 158, 197, 212
BstDSI CCRYGG 1 cut(s) 448
BstKTI GATC 5 cut(s) 64, 381, 445, 457, 465
BstMBI GATC 5 cut(s) 61, 378, 442, 454, 462
BstMWI GCNNNNNNNGC 4 cut(s) 75, 118, 180, 248
BstV1I GCAGC 2 cut(s) 141, 216
BstV2I GAAGAC 2 cut(s) 90, 403
Bsu15I ATCGAT 1 cut(s) 465
BsuRI GGCC 1 cut(s) 190
BsuTUI ATCGAT 1 cut(s) 465
BtgI CCRYGG 1 cut(s) 448
CciI TCATGA 1 cut(s) 497
ClaI ATCGAT 1 cut(s) 465
CviAII CATG 3 cut(s) 297, 432, 498
CviJI RGCY 3 cut(s) 154, 190, 320
CviKI_1 RGCY 3 cut(s) 154, 190, 320
DdeI CTNAG 3 cut(s) 158, 197, 212
DpnI GATC 5 cut(s) 63, 380, 444, 456, 464
DpnII GATC 5 cut(s) 61, 378, 442, 454, 462
Eam1104I CTCTTC 1 cut(s) 31
EarI CTCTTC 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 96
EcoT14I CCWWGG 1 cut(s) 96
EcoT22I ATGCAT 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 3 cut(s) 300, 435, 501
FaiI YATR 8 cut(s) 57, 123, 238, 240, 244, 298, 433, 499
FaqI GGGAC 1 cut(s) 58
FatI CATG 3 cut(s) 296, 431, 497
FblI GTMKAC 1 cut(s) 220
Fnu4HI GCNGC 2 cut(s) 155, 205
Fsp4HI GCNGC 2 cut(s) 155, 205
FspBI CTAG 1 cut(s) 317
GluI GCNGC 2 cut(s) 155, 205
HaeIII GGCC 1 cut(s) 190
Hin1II CATG 3 cut(s) 300, 435, 501
HinfI GANTC 4 cut(s) 146, 167, 214, 313
HphI GGTGA 3 cut(s) 112, 419, 472
Hpy166II GTNNAC 1 cut(s) 221
Hpy188I TCNGA 3 cut(s) 161, 198, 213
Hpy188III TCNNGA 2 cut(s) 437, 498
Hpy8I GTNNAC 1 cut(s) 221
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 2 cut(s) 35, 470
HpyCH4V TGCA 4 cut(s) 69, 183, 242, 296
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 118, 180, 248
HpyF3I CTNAG 3 cut(s) 158, 197, 212
Hsp92II CATG 3 cut(s) 300, 435, 501
Kzo9I GATC 5 cut(s) 61, 378, 442, 454, 462
LpnPI CCDG 7 cut(s) 29, 169, 184, 267, 342, 443, 450
Lsp1109I GCAGC 2 cut(s) 141, 216
LweI GCATC 1 cut(s) 99
MaeI CTAG 1 cut(s) 317
MaeIII GTNAC 2 cut(s) 31, 418
MalI GATC 5 cut(s) 63, 380, 444, 456, 464
MboI GATC 5 cut(s) 61, 378, 442, 454, 462
MboII GAAGA 4 cut(s) 18, 50, 95, 403
MluCI AATT 1 cut(s) 261
MlyI GAGTC 2 cut(s) 155, 223
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 6 cut(s) 96, 180, 186, 349, 360, 397
Mph1103I ATGCAT 1 cut(s) 244
MseI TTAA 1 cut(s) 260
MwoI GCNNNNNNNGC 4 cut(s) 75, 118, 180, 248
NdeII GATC 5 cut(s) 61, 378, 442, 454, 462
NlaIII CATG 3 cut(s) 300, 435, 501
NmuCI GTSAC 2 cut(s) 31, 418
NsiI ATGCAT 1 cut(s) 244
PagI TCATGA 1 cut(s) 497
PfeI GAWTC 2 cut(s) 167, 313
PkrI GCNGC 2 cut(s) 156, 206
PleI GAGTC 2 cut(s) 154, 222
PpsI GAGTC 2 cut(s) 154, 222
SaqAI TTAA 1 cut(s) 260
SatI GCNGC 2 cut(s) 155, 205
Sau3AI GATC 5 cut(s) 61, 378, 442, 454, 462
SchI GAGTC 2 cut(s) 155, 223
SetI ASST 5 cut(s) 102, 128, 286, 322, 462
SfaNI GCATC 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 484
SmoI CTYRAG 1 cut(s) 484
Sse9I AATT 1 cut(s) 261
SspMI CTAG 1 cut(s) 317
StyI CCWWGG 1 cut(s) 96
TaqI TCGA 2 cut(s) 144, 465
TasI AATT 1 cut(s) 261
TfiI GAWTC 2 cut(s) 167, 313
Tru1I TTAA 1 cut(s) 260
Tru9I TTAA 1 cut(s) 260
TseFI GTSAC 2 cut(s) 31, 418
TseI GCWGC 2 cut(s) 154, 204
Tsp45I GTSAC 2 cut(s) 31, 418
TspDTI ATGAA 1 cut(s) 483
TspGWI ACGGA 2 cut(s) 19, 65
XmiI GTMKAC 1 cut(s) 220
XspI CTAG 1 cut(s) 317
Zsp2I ATGCAT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.