RLG00000021486

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001784
Physical Location & Seq
Forward (+)
75816910 .. 75817350
441 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021486

Sequence Viewer

Length: 441 bp
ATGTCTGACTTTTCCAACCCACGTACTAGAGATCAAATAATAGCGGAAACTCCCGTACTCGCCTTACCTACACCAGCCATTGACGTCAGTGCATCAGGGCAACAATTACTTGTTTATATTGATTTTGTATTAGCTGACCAGCCAACCGCGGCGGTGGGGGCATCAAAGGCATCCATAGAAGACATGCCGAGAGTGATAGTCACCGAGGAAGGAACGGAGTGCAGTATATGCTTGGAAAGCTTCAAAGTCTGCGCTGAAGCCAAAGAGATGCCTTGTAAGCACAGGTTTCACTCGCACTGTATTGAAAACTGGCTGTACAGGCGTGCTTCCTGCCCACTATGCAGGTTCACCATGCCCGTTGACTTGGAAGAAGAGACGAATGGCATGAGCGAACATTGGATTGATATCACGGATTTTTTGGATTTTAGGGATATGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

147

Amino Acids

16.46

Weight (kDa)

4.55

Isoelectric Point (pI)

49.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 73 - 115 5e-13 Ring finger domain
zf-RING_11 PF17123 73 - 101 8.7e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 73 - 114 5.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 74 - 114 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 74 - 112 8.9e-06 RING-type zinc-finger
zf-rbx1 PF12678 82 - 115 8.2e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 333
AccII CGCG 1 cut(s) 149
AciI CCGC 4 cut(s) 44, 147, 149, 152
AcuI CTGAAG 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 3 cut(s) 25, 57, 317
AgsI TTSAA 2 cut(s) 244, 305
AluBI AGCT 2 cut(s) 134, 240
AluI AGCT 2 cut(s) 134, 240
Alw26I GTCTC 1 cut(s) 368
AspLEI GCGC 1 cut(s) 254
AsuHPI GGTGA 2 cut(s) 193, 340
BbsI GAAGAC 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 368
BfaI CTAG 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 333
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
BmsI GCATC 4 cut(s) 101, 170, 179, 258
BpiI GAAGAC 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 23
BsaBI GATNNNNATC 1 cut(s) 404
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 147, 204
Bse1I ACTGG 1 cut(s) 314
Bse8I GATNNNNATC 1 cut(s) 404
BseDI CCNNGG 2 cut(s) 147, 204
BseGI GGATG 1 cut(s) 170
BseJI GATNNNNATC 1 cut(s) 404
BseNI ACTGG 1 cut(s) 314
BsgI GTGCAG 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 149
BsmAI GTCTC 1 cut(s) 368
BsmBI CGTCTC 1 cut(s) 368
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 1 cut(s) 31
BspACI CCGC 4 cut(s) 44, 147, 149, 152
BspFNI CGCG 1 cut(s) 149
BspMI ACCTGC 1 cut(s) 333
BsrGI TGTACA 1 cut(s) 315
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 2 cut(s) 147, 204
BssMI GATC 1 cut(s) 31
BssNI GRCGYC 1 cut(s) 84
Bst4CI ACNGT 1 cut(s) 299
Bst6I CTCTTC 1 cut(s) 366
BstACI GRCGYC 1 cut(s) 84
BstAPI GCANNNNNTGC 1 cut(s) 228
BstAUI TGTACA 1 cut(s) 315
BstBAI YACGTR 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 1 cut(s) 170
BstFNI CGCG 1 cut(s) 149
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 1 cut(s) 31
BstMWI GCNNNNNNNGC 7 cut(s) 158, 167, 228, 237, 277, 319, 339
BstNSI RCATGY 1 cut(s) 187
BstUI CGCG 1 cut(s) 149
BstV2I GAAGAC 1 cut(s) 186
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 1 cut(s) 170
BtsIMutI CAGTG 2 cut(s) 94, 295
BveI ACCTGC 1 cut(s) 333
Cac8I GCNNGC 1 cut(s) 324
CfoI GCGC 1 cut(s) 254
Cfr42I CCGCGG 1 cut(s) 150
Csp6I GTAC 3 cut(s) 24, 56, 316
CviAII CATG 3 cut(s) 184, 352, 385
CviJI RGCY 6 cut(s) 77, 134, 142, 240, 260, 313
CviKI_1 RGCY 6 cut(s) 77, 134, 142, 240, 260, 313
CviQI GTAC 3 cut(s) 24, 56, 316
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Eco32I GATATC 1 cut(s) 406
Eco57I CTGAAG 1 cut(s) 276
EcoRV GATATC 1 cut(s) 406
Esp3I CGTCTC 1 cut(s) 368
FaeI CATG 3 cut(s) 187, 355, 388
FaiI YATR 9 cut(s) 117, 176, 185, 227, 229, 340, 353, 386, 434
FatI CATG 3 cut(s) 183, 351, 384
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 1 cut(s) 157
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 1 cut(s) 27
GlaI GCGC 1 cut(s) 253
GluI GCNGC 1 cut(s) 150
HhaI GCGC 1 cut(s) 254
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 3 cut(s) 187, 355, 388
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 238
HphI GGTGA 2 cut(s) 193, 340
Hpy166II GTNNAC 2 cut(s) 348, 361
Hpy188I TCNGA 1 cut(s) 7
Hpy8I GTNNAC 2 cut(s) 348, 361
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4IV ACGT 2 cut(s) 22, 84
HpyCH4V TGCA 3 cut(s) 92, 222, 342
HpyF10VI GCNNNNNNNGC 7 cut(s) 158, 167, 228, 237, 277, 319, 339
HpySE526I ACGT 2 cut(s) 22, 84
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 3 cut(s) 187, 355, 388
HspAI GCGC 1 cut(s) 252
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 1 cut(s) 31
LpnPI CCDG 8 cut(s) 81, 87, 152, 268, 295, 304, 328, 343
LweI GCATC 4 cut(s) 101, 170, 179, 258
MaeI CTAG 1 cut(s) 27
MaeII ACGT 2 cut(s) 22, 84
MaeIII GTNAC 1 cut(s) 199
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MboII GAAGA 3 cut(s) 191, 380, 383
MluCI AATT 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 1 cut(s) 199
MspA1I CMGCKG 1 cut(s) 149
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 7 cut(s) 158, 167, 228, 237, 277, 319, 339
NdeII GATC 1 cut(s) 31
NlaIII CATG 3 cut(s) 187, 355, 388
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 199
NspI RCATGY 1 cut(s) 187
PkrI GCNGC 1 cut(s) 151
Ppu21I YACGTR 1 cut(s) 23
RsaI GTAC 3 cut(s) 25, 57, 317
RsaNI GTAC 3 cut(s) 24, 56, 316
SacII CCGCGG 1 cut(s) 150
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 1 cut(s) 31
SetI ASST 7 cut(s) 25, 70, 87, 136, 242, 287, 347
SfaNI GCATC 4 cut(s) 101, 170, 179, 258
Sfr303I CCGCGG 1 cut(s) 150
SgrBI CCGCGG 1 cut(s) 150
Sse9I AATT 1 cut(s) 104
SsiI CCGC 4 cut(s) 44, 147, 149, 152
SspMI CTAG 1 cut(s) 27
TaaI ACNGT 1 cut(s) 299
TaiI ACGT 2 cut(s) 25, 87
TasI AATT 1 cut(s) 104
TatI WGTACW 1 cut(s) 315
TauI GCSGC 1 cut(s) 152
TscAI CASTG 2 cut(s) 94, 302
TseFI GTSAC 1 cut(s) 199
Tsp45I GTSAC 1 cut(s) 199
TspGWI ACGGA 2 cut(s) 230, 425
TspRI CASTG 2 cut(s) 94, 302
XceI RCATGY 1 cut(s) 187
XspI CTAG 1 cut(s) 27
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.