Rw2G049940

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001784
Physical Location & Seq
Reverse (-)
77499347 .. 77499787
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049940.1

Sequence Viewer

Length: 441 bp
ATGTCTGACTTTTCCAACCCACGTACTAGTGATCAAATGATAGGGGAAACTCCCGTACTCGCCTTACCTACACCAGCCATTGACGTCAGTGCATCAAGGCAACAATTACTTGCTTTAATTGATTTTGTATTCGCTGATCAGCCTACCGTGGTGGTGGGAGCATCAAAGGCATCCATAGAAGACATGCCGAGGGTGATAGTCACCGACGAAGGAATGGAGTGCAGTATATGTTTGGAAAGCTTCAAAGTCTGCGGTGAAGCCAAGGAGATGCCTTGTAAGCATAGGTTTCACTCGCACTGTATTGAAAACTGGCTGTACAGGCGTGCTTCCTGCCCACTTTGCAGGTTCACCGTGCCCGTTGACTTGGAAGAAGAGACGAATGGCATGAGCGAACATTGGATTGATATCACAGATTTTTTGGATTTTAGGGATATGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

146

Amino Acids

16.48

Weight (kDa)

4.53

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 73 - 115 1.1e-12 Ring finger domain
zf-RING_11 PF17123 73 - 101 6.6e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 73 - 114 8.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 74 - 114 1.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 74 - 112 3.6e-06 RING-type zinc-finger
zf-rbx1 PF12678 90 - 115 9e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 333
AciI CCGC 1 cut(s) 252
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 3 cut(s) 25, 57, 317
AgsI TTSAA 2 cut(s) 244, 305
AhlI ACTAGT 1 cut(s) 26
AluBI AGCT 1 cut(s) 240
AluI AGCT 1 cut(s) 240
Alw26I GTCTC 1 cut(s) 368
AsuHPI GGTGA 4 cut(s) 193, 205, 266, 340
BaeGI GKGCMC 1 cut(s) 357
BbsI GAAGAC 1 cut(s) 186
BclI TGATCA 2 cut(s) 31, 136
BcoDI GTCTC 1 cut(s) 368
BcuI ACTAGT 1 cut(s) 26
BfaI CTAG 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 333
BmsI GCATC 4 cut(s) 101, 170, 179, 258
BpiI GAAGAC 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 23
BsaBI GATNNNNATC 1 cut(s) 404
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 147, 188, 261
Bse1I ACTGG 1 cut(s) 314
Bse8I GATNNNNATC 1 cut(s) 404
BseDI CCNNGG 3 cut(s) 147, 188, 261
BseGI GGATG 1 cut(s) 170
BseJI GATNNNNATC 1 cut(s) 404
BseNI ACTGG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 357
BsgI GTGCAG 1 cut(s) 241
BsmAI GTCTC 1 cut(s) 368
BsmBI CGTCTC 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 357
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 2 cut(s) 31, 136
BspACI CCGC 1 cut(s) 252
BspMI ACCTGC 1 cut(s) 333
BsrGI TGTACA 1 cut(s) 315
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 3 cut(s) 147, 188, 261
BssMI GATC 2 cut(s) 31, 136
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 261
Bst4CI ACNGT 3 cut(s) 148, 299, 352
Bst6I CTCTTC 1 cut(s) 366
BstACI GRCGYC 1 cut(s) 84
BstAUI TGTACA 1 cut(s) 315
BstBAI YACGTR 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 1 cut(s) 170
BstKTI GATC 2 cut(s) 34, 139
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 2 cut(s) 31, 136
BstMWI GCNNNNNNNGC 4 cut(s) 167, 277, 319, 339
BstNSI RCATGY 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 357
BstV2I GAAGAC 1 cut(s) 186
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 1 cut(s) 170
BtsIMutI CAGTG 2 cut(s) 94, 295
BveI ACCTGC 1 cut(s) 333
Cac8I GCNNGC 1 cut(s) 324
Csp6I GTAC 3 cut(s) 24, 56, 316
CviAII CATG 2 cut(s) 184, 385
CviJI RGCY 5 cut(s) 77, 142, 240, 260, 313
CviKI_1 RGCY 5 cut(s) 77, 142, 240, 260, 313
CviQI GTAC 3 cut(s) 24, 56, 316
DpnI GATC 2 cut(s) 33, 138
DpnII GATC 2 cut(s) 31, 136
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 261
Eco32I GATATC 1 cut(s) 406
EcoRV GATATC 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
Esp3I CGTCTC 1 cut(s) 368
FaeI CATG 2 cut(s) 187, 388
FaiI YATR 7 cut(s) 176, 185, 227, 229, 282, 386, 434
FatI CATG 2 cut(s) 183, 384
FbaI TGATCA 2 cut(s) 31, 136
FokI GGATG 1 cut(s) 157
FspBI CTAG 1 cut(s) 27
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 2 cut(s) 187, 388
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 238
HphI GGTGA 4 cut(s) 193, 205, 266, 340
Hpy166II GTNNAC 2 cut(s) 348, 361
Hpy188I TCNGA 1 cut(s) 7
Hpy8I GTNNAC 2 cut(s) 348, 361
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 3 cut(s) 148, 299, 352
HpyCH4IV ACGT 2 cut(s) 22, 84
HpyCH4V TGCA 3 cut(s) 92, 222, 342
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 277, 319, 339
HpySE526I ACGT 2 cut(s) 22, 84
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 2 cut(s) 187, 388
Ksp22I TGATCA 2 cut(s) 31, 136
Kzo9I GATC 2 cut(s) 31, 136
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 5 cut(s) 87, 295, 304, 328, 343
LweI GCATC 4 cut(s) 101, 170, 179, 258
MaeI CTAG 1 cut(s) 27
MaeII ACGT 2 cut(s) 22, 84
MaeIII GTNAC 1 cut(s) 199
MalI GATC 2 cut(s) 33, 138
MboI GATC 2 cut(s) 31, 136
MboII GAAGA 3 cut(s) 191, 380, 383
MhlI GDGCHC 1 cut(s) 357
MluCI AATT 2 cut(s) 104, 117
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 1 cut(s) 183
MseI TTAA 1 cut(s) 116
MwoI GCNNNNNNNGC 4 cut(s) 167, 277, 319, 339
NdeII GATC 2 cut(s) 31, 136
NlaIII CATG 2 cut(s) 187, 388
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 199
NspI RCATGY 1 cut(s) 187
Ppu21I YACGTR 1 cut(s) 23
RsaI GTAC 3 cut(s) 25, 57, 317
RsaNI GTAC 3 cut(s) 24, 56, 316
SaqAI TTAA 1 cut(s) 116
Sau3AI GATC 2 cut(s) 31, 136
SduI GDGCHC 1 cut(s) 357
SetI ASST 6 cut(s) 25, 70, 87, 242, 287, 347
SfaNI GCATC 4 cut(s) 101, 170, 179, 258
SpeI ACTAGT 1 cut(s) 26
Sse9I AATT 2 cut(s) 104, 117
SsiI CCGC 1 cut(s) 252
SspMI CTAG 1 cut(s) 27
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 3 cut(s) 148, 299, 352
TaiI ACGT 2 cut(s) 25, 87
TasI AATT 2 cut(s) 104, 117
TatI WGTACW 1 cut(s) 315
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 2 cut(s) 94, 302
TseFI GTSAC 1 cut(s) 199
Tsp45I GTSAC 1 cut(s) 199
TspRI CASTG 2 cut(s) 94, 302
XceI RCATGY 1 cut(s) 187
XspI CTAG 1 cut(s) 27
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.