MD00G1131000.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0001784
Physical Location & Seq
Forward (+)
28229503 .. 28229904
402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1131000.v1.1.491

Sequence Viewer

Length: 402 bp
ATGGCAGAAGAAGGAGGACCACCCCAACCCAACAGTTTCCTCCACTCTTATTTTTATCCAAATCCCCCGCCCGCCGCTGCCTTGGCGGCTGTGGCGAGCTTGTCGTTGGTGACGGCCTTGGCCGGGGCGACCCAGGCGTCGATAGAGGCCTTGCCGAAGGTGACTGTGACGGAGGAAACGTCGGCGGAGTGCTGTATCTGCCTGAAGAGGTTGGAGGTCGGGGAGGAAGCCAAGGAGATGCCATGTAACCATAGGTTTCATCCGGTTTGCATTGATAGCTGGCTCGAGAGGCGTCCTTCCTGCCCGCTTTGCCGGTTTTCGATGCCTACGAGGGCGGAGGCGGCCGCACAAGGGCGAGGACATCAGACCAACAGGTGGGATTCGATTGAAATGGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

134

Amino Acids

14.47

Weight (kDa)

5.34

Isoelectric Point (pI)

65.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 61 - 105 2.6e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 63 - 105 3.2e-14 Ring finger domain
zf-RING_11 PF17123 63 - 91 8.5e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 63 - 104 1.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 64 - 104 8.8e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 136
AccB7I CCANNNNNTGG 1 cut(s) 375
AciI CCGC 9 cut(s) 68, 72, 75, 86, 185, 305, 335, 341, 345
AcoI YGGCCR 2 cut(s) 120, 342
AcuI CTGAAG 1 cut(s) 224
AcyI GRCGYC 2 cut(s) 137, 292
AfiI CCNNNNNNNGG 3 cut(s) 123, 351, 375
AgsI TTSAA 1 cut(s) 389
AjnI CCWGG 1 cut(s) 132
AluBI AGCT 2 cut(s) 99, 279
AluI AGCT 2 cut(s) 99, 279
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 4 cut(s) 114, 120, 147, 342
ApeKI GCWGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 17
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 121, 172
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 1 cut(s) 17
BbvI GCAGC 1 cut(s) 64
BceAI ACGGC 1 cut(s) 129
BciT130I CCWGG 1 cut(s) 134
BcnI CCSGG 1 cut(s) 124
BglI GCCNNNNNGGC 1 cut(s) 86
BisI GCNGC 5 cut(s) 75, 78, 87, 342, 345
BlsI GCNGC 5 cut(s) 76, 79, 88, 343, 346
Bme1390I CCNGG 2 cut(s) 124, 134
Bme18I GGWCC 1 cut(s) 17
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 17
BmrFI CCNGG 2 cut(s) 124, 134
BmsI GCATC 2 cut(s) 228, 312
BpuMI CCSGG 1 cut(s) 124
BsaHI GRCGYC 2 cut(s) 137, 292
BsaJI CCNNGG 5 cut(s) 81, 117, 123, 132, 231
BsaWI WCCGGW 1 cut(s) 262
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 351, 375
Bse118I RCCGGY 1 cut(s) 312
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 5 cut(s) 81, 117, 123, 132, 231
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 3 cut(s) 123, 351, 375
BseX3I CGGCCG 1 cut(s) 342
BseXI GCAGC 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 4 cut(s) 116, 122, 149, 344
BsiEI CGRYCG 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 3 cut(s) 123, 263, 313
BslI CCNNNNNNNGG 3 cut(s) 123, 351, 375
BsnI GGCC 4 cut(s) 116, 122, 149, 344
BsoBI CYCGRG 1 cut(s) 284
BspACI CCGC 9 cut(s) 68, 72, 75, 86, 185, 305, 335, 341, 345
BspANI GGCC 4 cut(s) 116, 122, 149, 344
BsrFI RCCGGY 1 cut(s) 312
BssAI RCCGGY 1 cut(s) 312
BssECI CCNNGG 5 cut(s) 81, 117, 123, 132, 231
BssNI GRCGYC 2 cut(s) 137, 292
BssT1I CCWWGG 3 cut(s) 81, 117, 231
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 2 cut(s) 35, 166
Bst6I CTCTTC 1 cut(s) 200
BstACI GRCGYC 2 cut(s) 137, 292
BstC8I GCNNGC 4 cut(s) 72, 97, 281, 305
BstF5I GGATG 1 cut(s) 259
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 9 cut(s) 83, 86, 92, 134, 198, 276, 289, 309, 341
BstNI CCWGG 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 122, 132
BstV1I GCAGC 1 cut(s) 64
BstZI CGGCCG 1 cut(s) 342
BsuRI GGCC 4 cut(s) 116, 122, 149, 344
BtsCI GGATG 1 cut(s) 259
Cac8I GCNNGC 4 cut(s) 72, 97, 281, 305
CciNI GCGGCCGC 1 cut(s) 342
Cfr10I RCCGGY 1 cut(s) 312
Cfr13I GGNCC 1 cut(s) 17
CseI GACGC 2 cut(s) 126, 281
CviAII CATG 1 cut(s) 243
CviJI RGCY 9 cut(s) 89, 99, 116, 122, 149, 230, 279, 283, 344
CviKI_1 RGCY 9 cut(s) 89, 99, 116, 122, 149, 230, 279, 283, 344
DrdI GACNNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 136
EaeI YGGCCR 2 cut(s) 120, 342
EagI CGGCCG 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
EciI GGCGGA 2 cut(s) 200, 350
EclXI CGGCCG 1 cut(s) 342
Eco130I CCWWGG 3 cut(s) 81, 117, 231
Eco147I AGGCCT 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 17
Eco52I CGGCCG 1 cut(s) 342
Eco57I CTGAAG 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 132
EcoT14I CCWWGG 3 cut(s) 81, 117, 231
ErhI CCWWGG 3 cut(s) 81, 117, 231
FaeI CATG 1 cut(s) 246
FaiI YATR 2 cut(s) 244, 252
FatI CATG 1 cut(s) 242
FauI CCCGC 3 cut(s) 75, 79, 312
Fnu4HI GCNGC 5 cut(s) 75, 78, 87, 342, 345
FokI GGATG 1 cut(s) 246
Fsp4HI GCNGC 5 cut(s) 75, 78, 87, 342, 345
GluI GCNGC 5 cut(s) 75, 78, 87, 342, 345
HaeIII GGCC 4 cut(s) 116, 122, 149, 344
HapII CCGG 3 cut(s) 123, 263, 313
HgaI GACGC 2 cut(s) 126, 281
Hin1I GRCGYC 2 cut(s) 137, 292
Hin1II CATG 1 cut(s) 246
HinfI GANTC 1 cut(s) 380
HpaII CCGG 3 cut(s) 123, 263, 313
HphI GGTGA 2 cut(s) 121, 172
Hpy188I TCNGA 1 cut(s) 366
Hpy188III TCNNGA 1 cut(s) 286
Hpy99I CGWCG 2 cut(s) 142, 184
HpyAV CCTTC 3 cut(s) 5, 151, 306
HpyCH4III ACNGT 2 cut(s) 35, 166
HpyCH4IV ACGT 1 cut(s) 179
HpyCH4V TGCA 1 cut(s) 270
HpyF10VI GCNNNNNNNGC 9 cut(s) 83, 86, 92, 134, 198, 276, 289, 309, 341
HpySE526I ACGT 1 cut(s) 179
Hsp92I GRCGYC 2 cut(s) 137, 292
Hsp92II CATG 1 cut(s) 246
LpnPI CCDG 9 cut(s) 119, 136, 146, 215, 265, 276, 313, 326, 358
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 2 cut(s) 228, 312
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 4 cut(s) 109, 160, 166, 245
MboII GAAGA 2 cut(s) 20, 217
MmeI TCCRAC 1 cut(s) 192
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 3 cut(s) 123, 263, 313
MspR9I CCNGG 2 cut(s) 124, 134
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 9 cut(s) 83, 86, 92, 134, 198, 276, 289, 309, 341
NciI CCSGG 1 cut(s) 124
NlaIII CATG 1 cut(s) 246
NmuCI GTSAC 3 cut(s) 109, 160, 166
NotI GCGGCCGC 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 284
PceI AGGCCT 1 cut(s) 149
PcsI WCGNNNNNNNCGW 2 cut(s) 176, 326
PfeI GAWTC 1 cut(s) 380
PflMI CCANNNNNTGG 1 cut(s) 375
PkrI GCNGC 5 cut(s) 76, 79, 88, 343, 346
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspPI GGNCC 1 cut(s) 17
SatI GCNGC 5 cut(s) 75, 78, 87, 342, 345
Sau96I GGNCC 1 cut(s) 17
ScrFI CCNGG 2 cut(s) 124, 134
SetI ASST 8 cut(s) 101, 162, 182, 212, 219, 257, 281, 377
SfaNI GCATC 2 cut(s) 228, 312
Sfr274I CTCGAG 1 cut(s) 284
SinI GGWCC 1 cut(s) 17
SlaI CTCGAG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 9 cut(s) 68, 72, 75, 86, 185, 305, 335, 341, 345
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 2 cut(s) 122, 132
StyI CCWWGG 3 cut(s) 81, 117, 231
TaaI ACNGT 2 cut(s) 35, 166
TaiI ACGT 1 cut(s) 182
TaqI TCGA 4 cut(s) 140, 285, 320, 383
TauI GCSGC 4 cut(s) 77, 89, 344, 347
TfiI GAWTC 1 cut(s) 380
TseFI GTSAC 3 cut(s) 109, 160, 166
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 3 cut(s) 109, 160, 166
TspDTI ATGAA 1 cut(s) 248
TspGWI ACGGA 1 cut(s) 185
Van91I CCANNNNNTGG 1 cut(s) 375
VpaK11BI GGWCC 1 cut(s) 17
XhoI CTCGAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.