MD17G1031000.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001784
Physical Location & Seq
Forward (+)
2246405 .. 2246824
420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1031000.v1.1.491

Sequence Viewer

Length: 420 bp
ATGATGAACGACGGTGACGAGGAAGCACCGTTCGACCTCGAGATCCCACTTCATCTCCGTCGTTTGCAGATGGAGGTAGCACGACGGCACATACGGCGCTATTTAGAAGCAAGAGACGACAATCTCATCCATTCCAATATCAATTATTTCAACCATAACATTGGCAATCGTTTTCGTGAGCCGTCGTCGGCTGCGGCGGCGGCGGCATCCAAGGCGTCCATAGACGCCATGCCGAGAGTGACGATCACGGTGGTCGGCGCCGACTGCGCTATCTGCATGGGGGACTTGGAAATCGGCGGTGAAGCCAGAGAAATGCCTTGTAAGCACAGGTTTCACTCGCCTTGTATCGAAGCGTGGCTGTGCCGGCGAGCATCCTGCCCGCTTTGCAGGTTCCCTGTTCCCAAGGAAGAACACCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

140

Amino Acids

15.81

Weight (kDa)

6.43

Isoelectric Point (pI)

76.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 88 - 130 1.6e-12 Ring finger domain
zf-RING_11 PF17123 88 - 116 8e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 89 - 129 6.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 89 - 129 9.5e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 378
AccB1I GGYRCC 1 cut(s) 257
AciI CCGC 6 cut(s) 194, 197, 200, 203, 297, 380
AclWI GGATC 1 cut(s) 37
AcyI GRCGYC 3 cut(s) 215, 225, 258
AgsI TTSAA 1 cut(s) 151
AloI GAACNNNNNNTCC 2 cut(s) 14, 46
Alw26I GTCTC 1 cut(s) 108
AlwI GGATC 1 cut(s) 37
Ama87I CYCGRG 1 cut(s) 38
ApeKI GCWGC 1 cut(s) 191
AspLEI GCGC 3 cut(s) 99, 260, 269
AsuHPI GGTGA 2 cut(s) 26, 311
AvaI CYCGRG 1 cut(s) 38
BanI GGYRCC 1 cut(s) 257
BbvI GCAGC 1 cut(s) 178
BccI CCATC 1 cut(s) 64
BceAI ACGGC 3 cut(s) 101, 110, 166
BcgI CGANNNNNNTGC 2 cut(s) 357, 391
BcoDI GTCTC 1 cut(s) 108
BfoI RGCGCY 2 cut(s) 100, 261
BfuAI ACCTGC 1 cut(s) 378
BisI GCNGC 5 cut(s) 192, 195, 198, 201, 204
BlsI GCNGC 5 cut(s) 193, 196, 199, 202, 205
BmeT110I CYCGRG 1 cut(s) 38
BmiI GGNNCC 2 cut(s) 259, 392
BmsI GCATC 2 cut(s) 215, 380
BsaHI GRCGYC 3 cut(s) 215, 225, 258
BsaJI CCNNGG 2 cut(s) 210, 402
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bse118I RCCGGY 1 cut(s) 363
BseDI CCNNGG 2 cut(s) 210, 402
BseGI GGATG 3 cut(s) 126, 206, 371
BseXI GCAGC 1 cut(s) 178
BshNI GGYRCC 1 cut(s) 257
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 1 cut(s) 364
BslFI GGGAC 1 cut(s) 296
BsmAI GTCTC 1 cut(s) 108
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 296
BsoBI CYCGRG 1 cut(s) 38
Bsp143I GATC 2 cut(s) 42, 243
BspACI CCGC 6 cut(s) 194, 197, 200, 203, 297, 380
BspLI GGNNCC 2 cut(s) 259, 392
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 257
BsrFI RCCGGY 1 cut(s) 363
BssAI RCCGGY 1 cut(s) 363
BssECI CCNNGG 2 cut(s) 210, 402
BssMI GATC 2 cut(s) 42, 243
BssNI GRCGYC 3 cut(s) 215, 225, 258
BssT1I CCWWGG 2 cut(s) 210, 402
Bst4CI ACNGT 3 cut(s) 14, 30, 250
BstACI GRCGYC 3 cut(s) 215, 225, 258
BstC8I GCNNGC 3 cut(s) 365, 369, 380
BstF5I GGATG 3 cut(s) 126, 206, 371
BstH2I RGCGCY 2 cut(s) 100, 261
BstHHI GCGC 3 cut(s) 99, 260, 269
BstKTI GATC 2 cut(s) 45, 246
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 2 cut(s) 42, 243
BstV1I GCAGC 1 cut(s) 178
BstX2I RGATCY 1 cut(s) 42
BstXI CCANNNNNNTGG 1 cut(s) 161
BstYI RGATCY 1 cut(s) 42
BtsCI GGATG 3 cut(s) 126, 206, 371
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 3 cut(s) 365, 369, 380
CfoI GCGC 3 cut(s) 99, 260, 269
Cfr10I RCCGGY 1 cut(s) 363
CseI GACGC 2 cut(s) 204, 233
CviAII CATG 2 cut(s) 229, 277
CviJI RGCY 4 cut(s) 181, 191, 305, 358
CviKI_1 RGCY 4 cut(s) 181, 191, 305, 358
DinI GGCGCC 1 cut(s) 259
DpnI GATC 2 cut(s) 44, 245
DpnII GATC 2 cut(s) 42, 243
Eco130I CCWWGG 2 cut(s) 210, 402
Eco88I CYCGRG 1 cut(s) 38
EcoT14I CCWWGG 2 cut(s) 210, 402
EgeI GGCGCC 1 cut(s) 259
EheI GGCGCC 1 cut(s) 259
ErhI CCWWGG 2 cut(s) 210, 402
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 2 cut(s) 232, 280
FaiI YATR 5 cut(s) 92, 156, 221, 230, 278
FaqI GGGAC 1 cut(s) 296
FatI CATG 2 cut(s) 228, 276
FauI CCCGC 1 cut(s) 387
Fnu4HI GCNGC 5 cut(s) 192, 195, 198, 201, 204
FokI GGATG 3 cut(s) 113, 193, 358
Fsp4HI GCNGC 5 cut(s) 192, 195, 198, 201, 204
GlaI GCGC 3 cut(s) 98, 259, 268
GluI GCNGC 5 cut(s) 192, 195, 198, 201, 204
HaeII RGCGCY 2 cut(s) 100, 261
HapII CCGG 1 cut(s) 364
HgaI GACGC 2 cut(s) 204, 233
HhaI GCGC 3 cut(s) 99, 260, 269
Hin1I GRCGYC 3 cut(s) 215, 225, 258
Hin1II CATG 2 cut(s) 232, 280
Hin6I GCGC 3 cut(s) 97, 258, 267
HinP1I GCGC 3 cut(s) 97, 258, 267
HpaII CCGG 1 cut(s) 364
HphI GGTGA 2 cut(s) 26, 311
Hpy188III TCNNGA 2 cut(s) 40, 176
Hpy99I CGWCG 5 cut(s) 14, 63, 87, 187, 190
HpyCH4III ACNGT 3 cut(s) 14, 30, 250
HpyCH4V TGCA 3 cut(s) 67, 276, 387
Hsp92I GRCGYC 3 cut(s) 215, 225, 258
Hsp92II CATG 2 cut(s) 232, 280
HspAI GCGC 3 cut(s) 97, 258, 267
KasI GGCGCC 1 cut(s) 257
KroI GCCGGC 1 cut(s) 363
KroNI GCCGGC 1 cut(s) 365
Kzo9I GATC 2 cut(s) 42, 243
LpnPI CCDG 6 cut(s) 313, 319, 373, 377, 388, 408
Lsp1109I GCAGC 1 cut(s) 178
LweI GCATC 2 cut(s) 215, 380
MaeIII GTNAC 2 cut(s) 14, 238
MalI GATC 2 cut(s) 44, 245
MboI GATC 2 cut(s) 42, 243
MboII GAAGA 1 cut(s) 419
MflI RGATCY 1 cut(s) 42
MluCI AATT 1 cut(s) 142
Mly113I GGCGCC 1 cut(s) 258
MnlI CCTC 3 cut(s) 13, 47, 67
MroNI GCCGGC 1 cut(s) 363
MspI CCGG 1 cut(s) 364
NaeI GCCGGC 1 cut(s) 365
NarI GGCGCC 1 cut(s) 258
NdeII GATC 2 cut(s) 42, 243
NgoMIV GCCGGC 1 cut(s) 363
NlaIII CATG 2 cut(s) 232, 280
NlaIV GGNNCC 2 cut(s) 259, 392
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 2 cut(s) 14, 238
PaeR7I CTCGAG 1 cut(s) 38
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PdiI GCCGGC 1 cut(s) 365
PkrI GCNGC 5 cut(s) 193, 196, 199, 202, 205
PluTI GGCGCC 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 259, 392
PsuI RGATCY 1 cut(s) 42
SatI GCNGC 5 cut(s) 192, 195, 198, 201, 204
Sau3AI GATC 2 cut(s) 42, 243
SetI ASST 4 cut(s) 39, 78, 332, 392
SfaNI GCATC 2 cut(s) 215, 380
SfoI GGCGCC 1 cut(s) 259
Sfr274I CTCGAG 1 cut(s) 38
SlaI CTCGAG 1 cut(s) 38
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 1 cut(s) 142
SsiI CCGC 6 cut(s) 194, 197, 200, 203, 297, 380
SspDI GGCGCC 1 cut(s) 257
StyI CCWWGG 2 cut(s) 210, 402
TaaI ACNGT 3 cut(s) 14, 30, 250
TaqI TCGA 3 cut(s) 33, 39, 348
TasI AATT 1 cut(s) 142
TauI GCSGC 4 cut(s) 197, 200, 203, 206
TseFI GTSAC 2 cut(s) 14, 238
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 2 cut(s) 14, 238
TspDTI ATGAA 2 cut(s) 20, 41
TspGWI ACGGA 1 cut(s) 47
XhoI CTCGAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.