pycom111g04350

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0001784
Physical Location & Seq
Forward (+)
2627712 .. 2628266
555 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g04350.1

Sequence Viewer

Length: 555 bp
ATGTCTTCCCAATCGCAATCTGCTGGCTCTTCCTCCGATCAACCACCGAATACTACTACTCAACCGACAACGTCCTCCACACTACACGCACACGACGTCGACTCCGACTCCGACGATACGTCCGTAGTACTAGCCCTACCAGCTACCCCAGCGATGGCAGAAGTCGAAGCAGCATCCCAAACGTACAGTTTCCTCGACTCTTACTTTTATCCAAATCCCCCGCCTGCCGCTGCCTTTGCGGCCGTGGCGGGCCTGGCATTGGTGACGGCCTTGGCTGGGGCCACCCAGGAGTCGATAGAAGCCTTGCCGAAGGTGACGGTGACGGAGGAAACAATGGCGGAGTGCAGTATCTGTTTGAAGGAGTTGGAGGTTGGGGAGGAAGCCAAGGAGATGCCATGTAACCATAGGTTTCATCCGGTTTGCATTGTTAGCTGGCTCGAGAGGCGTCCTTCCTGCCCGCTTTGCCGGTTTTCGATGCCTACGAGGGCGGAGGCGGCCGCACAAGGGCAAGGACATCAAACCCCCGTGGTGGATTCGATTGAAATGGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

185

Amino Acids

19.47

Weight (kDa)

4.41

Isoelectric Point (pI)

63.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 111 - 156 3e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 114 - 156 3.7e-14 Ring finger domain
zf-RING_11 PF17123 114 - 142 5.6e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 114 - 155 2e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 115 - 155 2.4e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 99
AciI CCGC 9 cut(s) 221, 228, 239, 248, 338, 458, 488, 494, 498
AcoI YGGCCR 2 cut(s) 240, 495
AcyI GRCGYC 2 cut(s) 96, 445
AfaI GTAC 2 cut(s) 129, 185
AfiI CCNNNNNNNGG 5 cut(s) 154, 259, 276, 504, 529
AgsI TTSAA 2 cut(s) 358, 542
AhdI GACNNNNNGTC 1 cut(s) 118
AjnI CCWGG 2 cut(s) 252, 285
AluBI AGCT 2 cut(s) 143, 432
AluI AGCT 2 cut(s) 143, 432
AlwNI CAGNNNCTG 1 cut(s) 351
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 5 cut(s) 240, 250, 267, 279, 495
ApeKI GCWGC 2 cut(s) 170, 230
AspS9I GGNCC 2 cut(s) 250, 279
AsuHPI GGTGA 3 cut(s) 274, 325, 331
AvaI CYCGRG 1 cut(s) 437
BaeI ACNNNNGTAYC 2 cut(s) 108, 141
BbvI GCAGC 2 cut(s) 182, 217
BccI CCATC 1 cut(s) 148
BceAI ACGGC 2 cut(s) 227, 282
BciT130I CCWGG 2 cut(s) 254, 287
BfaI CTAG 1 cut(s) 131
BglI GCCNNNNNGGC 1 cut(s) 239
BisI GCNGC 6 cut(s) 171, 228, 231, 240, 495, 498
BlsI GCNGC 6 cut(s) 172, 229, 232, 241, 496, 499
BmcAI AGTACT 1 cut(s) 129
Bme1390I CCNGG 2 cut(s) 254, 287
BmeRI GACNNNNNGTC 1 cut(s) 118
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 2 cut(s) 250, 279
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 2 cut(s) 254, 287
BmsI GCATC 3 cut(s) 182, 381, 465
BsaHI GRCGYC 2 cut(s) 96, 445
BsaJI CCNNGG 5 cut(s) 243, 270, 285, 384, 525
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 4 cut(s) 86, 92, 116, 122
Bsc4I CCNNNNNNNGG 5 cut(s) 154, 259, 276, 504, 529
Bse118I RCCGGY 1 cut(s) 465
BseBI CCWGG 2 cut(s) 254, 287
BseDI CCNNGG 5 cut(s) 243, 270, 285, 384, 525
BseGI GGATG 2 cut(s) 173, 412
BseLI CCNNNNNNNGG 5 cut(s) 154, 259, 276, 504, 529
BseX3I CGGCCG 2 cut(s) 240, 495
BseXI GCAGC 2 cut(s) 182, 217
BseYI CCCAGC 2 cut(s) 148, 275
BsgI GTGCAG 1 cut(s) 364
Bsh1285I CGRYCG 2 cut(s) 243, 498
BshFI GGCC 5 cut(s) 242, 252, 269, 281, 497
BsiEI CGRYCG 2 cut(s) 243, 498
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 2 cut(s) 416, 466
BslI CCNNNNNNNGG 5 cut(s) 154, 259, 276, 504, 529
BsnI GGCC 5 cut(s) 242, 252, 269, 281, 497
BsoBI CYCGRG 1 cut(s) 437
Bsp143I GATC 1 cut(s) 37
BspACI CCGC 9 cut(s) 221, 228, 239, 248, 338, 458, 488, 494, 498
BspANI GGCC 5 cut(s) 242, 252, 269, 281, 497
BspLI GGNNCC 1 cut(s) 280
BspQI GCTCTTC 1 cut(s) 34
BsrFI RCCGGY 1 cut(s) 465
BssAI RCCGGY 1 cut(s) 465
BssECI CCNNGG 5 cut(s) 243, 270, 285, 384, 525
BssMI GATC 1 cut(s) 37
BssNI GRCGYC 2 cut(s) 96, 445
BssT1I CCWWGG 2 cut(s) 270, 384
Bst2UI CCWGG 2 cut(s) 254, 287
Bst4CI ACNGT 2 cut(s) 188, 319
Bst6I CTCTTC 1 cut(s) 34
BstACI GRCGYC 2 cut(s) 96, 445
BstC8I GCNNGC 5 cut(s) 25, 225, 250, 434, 458
BstDSI CCRYGG 2 cut(s) 243, 525
BstF5I GGATG 2 cut(s) 173, 412
BstKTI GATC 1 cut(s) 40
BstMBI GATC 1 cut(s) 37
BstMCI CGRYCG 2 cut(s) 243, 498
BstNI CCWGG 2 cut(s) 254, 287
BstSCI CCNGG 2 cut(s) 252, 285
BstV1I GCAGC 2 cut(s) 182, 217
BstZI CGGCCG 2 cut(s) 240, 495
BsuRI GGCC 5 cut(s) 242, 252, 269, 281, 497
BtgI CCRYGG 2 cut(s) 243, 525
BtgZI GCGATG 1 cut(s) 167
BtsCI GGATG 2 cut(s) 173, 412
Cac8I GCNNGC 5 cut(s) 25, 225, 250, 434, 458
CaiI CAGNNNCTG 1 cut(s) 351
CciNI GCGGCCGC 1 cut(s) 495
Cfr10I RCCGGY 1 cut(s) 465
Cfr13I GGNCC 2 cut(s) 250, 279
CseI GACGC 1 cut(s) 434
Csp6I GTAC 2 cut(s) 128, 184
CviAII CATG 1 cut(s) 396
CviQI GTAC 2 cut(s) 128, 184
DpnI GATC 1 cut(s) 39
DpnII GATC 1 cut(s) 37
DriI GACNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 2 cut(s) 240, 495
EagI CGGCCG 2 cut(s) 240, 495
Eam1104I CTCTTC 1 cut(s) 34
Eam1105I GACNNNNNGTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 34
EciI GGCGGA 2 cut(s) 353, 503
EclXI CGGCCG 2 cut(s) 240, 495
Eco130I CCWWGG 2 cut(s) 270, 384
Eco52I CGGCCG 2 cut(s) 240, 495
Eco88I CYCGRG 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 252, 285
EcoT14I CCWWGG 2 cut(s) 270, 384
ErhI CCWWGG 2 cut(s) 270, 384
FaeI CATG 1 cut(s) 399
FaiI YATR 2 cut(s) 397, 405
FatI CATG 1 cut(s) 395
FauI CCCGC 3 cut(s) 228, 241, 465
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 6 cut(s) 171, 228, 231, 240, 495, 498
FokI GGATG 2 cut(s) 160, 399
Fsp4HI GCNGC 6 cut(s) 171, 228, 231, 240, 495, 498
FspBI CTAG 1 cut(s) 131
GluI GCNGC 6 cut(s) 171, 228, 231, 240, 495, 498
GsaI CCCAGC 2 cut(s) 152, 279
HaeIII GGCC 5 cut(s) 242, 252, 269, 281, 497
HapII CCGG 2 cut(s) 416, 466
HgaI GACGC 1 cut(s) 434
Hin1I GRCGYC 2 cut(s) 96, 445
Hin1II CATG 1 cut(s) 399
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 5 cut(s) 101, 107, 197, 290, 533
HpaII CCGG 2 cut(s) 416, 466
HphI GGTGA 3 cut(s) 274, 325, 331
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 3 cut(s) 37, 106, 112
Hpy188III TCNNGA 1 cut(s) 439
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 3 cut(s) 98, 101, 116
HpyAV CCTTC 3 cut(s) 304, 352, 459
HpyCH4III ACNGT 2 cut(s) 188, 319
HpyCH4IV ACGT 4 cut(s) 71, 96, 119, 182
HpyCH4V TGCA 2 cut(s) 345, 423
HpySE526I ACGT 4 cut(s) 71, 96, 119, 182
Hsp92I GRCGYC 2 cut(s) 96, 445
Hsp92II CATG 1 cut(s) 399
Kzo9I GATC 1 cut(s) 37
LguI GCTCTTC 1 cut(s) 34
Lsp1109I GCAGC 2 cut(s) 182, 217
LweI GCATC 3 cut(s) 182, 381, 465
MaeI CTAG 1 cut(s) 131
MaeII ACGT 4 cut(s) 71, 96, 119, 182
MaeIII GTNAC 4 cut(s) 262, 313, 319, 398
MalI GATC 1 cut(s) 39
MboI GATC 1 cut(s) 37
MboII GAAGA 1 cut(s) 21
MlyI GAGTC 4 cut(s) 95, 101, 191, 299
MmeI TCCRAC 3 cut(s) 129, 135, 345
MnlI CCTC 9 cut(s) 43, 85, 203, 319, 361, 370, 435, 477, 484
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 2 cut(s) 416, 466
MspR9I CCNGG 2 cut(s) 254, 287
MvaI CCWGG 2 cut(s) 254, 287
NdeII GATC 1 cut(s) 37
NlaIII CATG 1 cut(s) 399
NlaIV GGNNCC 1 cut(s) 280
NmuCI GTSAC 3 cut(s) 262, 313, 319
NotI GCGGCCGC 1 cut(s) 495
PaeR7I CTCGAG 1 cut(s) 437
PciSI GCTCTTC 1 cut(s) 34
PcsI WCGNNNNNNNCGW 4 cut(s) 93, 102, 120, 479
PfeI GAWTC 1 cut(s) 533
PflFI GACNNNGTC 1 cut(s) 70
PkrI GCNGC 6 cut(s) 172, 229, 232, 241, 496, 499
PleI GAGTC 4 cut(s) 95, 101, 191, 298
PpsI GAGTC 4 cut(s) 95, 101, 191, 298
Psp6I CCWGG 2 cut(s) 252, 285
PspFI CCCAGC 2 cut(s) 148, 275
PspGI CCWGG 2 cut(s) 252, 285
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 2 cut(s) 250, 279
PstNI CAGNNNCTG 1 cut(s) 351
PsyI GACNNNGTC 1 cut(s) 70
RsaI GTAC 2 cut(s) 129, 185
RsaNI GTAC 2 cut(s) 128, 184
SalI GTCGAC 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 34
SatI GCNGC 6 cut(s) 171, 228, 231, 240, 495, 498
Sau3AI GATC 1 cut(s) 37
Sau96I GGNCC 2 cut(s) 250, 279
ScaI AGTACT 1 cut(s) 129
SchI GAGTC 4 cut(s) 95, 101, 191, 299
ScrFI CCNGG 2 cut(s) 254, 287
SetI ASST 9 cut(s) 74, 99, 122, 145, 185, 315, 372, 410, 434
SfaNI GCATC 3 cut(s) 182, 381, 465
Sfr274I CTCGAG 1 cut(s) 437
SlaI CTCGAG 1 cut(s) 437
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SsiI CCGC 9 cut(s) 221, 228, 239, 248, 338, 458, 488, 494, 498
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 2 cut(s) 252, 285
StyI CCWWGG 2 cut(s) 270, 384
TaaI ACNGT 2 cut(s) 188, 319
TaiI ACGT 4 cut(s) 74, 99, 122, 185
TaqI TCGA 7 cut(s) 99, 165, 195, 293, 438, 473, 536
TatI WGTACW 1 cut(s) 127
TauI GCSGC 4 cut(s) 230, 242, 497, 500
TfiI GAWTC 1 cut(s) 533
TseFI GTSAC 3 cut(s) 262, 313, 319
TseI GCWGC 2 cut(s) 170, 230
Tsp45I GTSAC 3 cut(s) 262, 313, 319
TspDTI ATGAA 1 cut(s) 401
TspGWI ACGGA 2 cut(s) 112, 338
Tth111I GACNNNGTC 1 cut(s) 70
XhoI CTCGAG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 1 cut(s) 131
ZraI GACGTC 1 cut(s) 97
ZrmI AGTACT 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.