Rw4G001380

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001784
Physical Location & Seq
Reverse (-)
2501251 .. 2501838
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G001380.1

Sequence Viewer

Length: 588 bp
ATGTCTAATTCCGAAGCAAACGACGATGGTCAATTGTCCCTATCGGAATCATTAGTCTCTTTCTCCGACCAACGCGTGCCAATATTGCACAGTTTTACTATCAGAGGGCCAATATTTACTGGAGGGCTACCTGGTGTCATCAATCGGACATCCTCTGTGTCGCGGCCAAGGGCATCCAAGGTCTCCATAGAGGCCATGCCAACTGTGACAGTGGCATCTGAGGAAGGTAATGAATGCAGTATCTGCTTGGATGGCTTGAAGGTCGGCGACGAAGCCAAGGAAATGCCTTGCAAGCACAGGTTCCACGGGGCCTGTATCAAAAAGTGGATGGGGATGAGCACTCTCTGCCCGCTTTGCAGGTTCTCTTTGCCGGCAGAGTCAGAAGAGGAAGAAAATAGTGATGGTGGTGACGATTATTTTAACCTTTTTAGGTCTATTCTTGATGTTGTTAACAATGGCAGTGATACTGAAGATGACTCTGAAAGTGAGGAAGATATCGGAGGTGAATGGGAATCAAGCACTGTCGATGATGATGATGCTTCAGTCGGAGATAATGTCACCTTCAGGATTAGTTTTAGTATTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

195

Amino Acids

21.17

Weight (kDa)

4.25

Isoelectric Point (pI)

58.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 77 - 120 6.3e-15 Ring finger domain
zf-RING_11 PF17123 78 - 106 3.3e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 78 - 119 1.3e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 79 - 119 1.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 96 - 120 2.3e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 348
AccII CGCG 2 cut(s) 75, 163
AciI CCGC 2 cut(s) 163, 350
AcoI YGGCCR 1 cut(s) 164
AcuI CTGAAG 3 cut(s) 489, 525, 547
AflIII ACRYGT 1 cut(s) 73
AgsI TTSAA 2 cut(s) 259, 584
AjnI CCWGG 1 cut(s) 130
Alw21I GWGCWC 1 cut(s) 341
Alw26I GTCTC 2 cut(s) 61, 187
AlwNI CAGNNNCTG 1 cut(s) 243
AoxI GGCC 4 cut(s) 107, 164, 192, 309
AspS9I GGNCC 2 cut(s) 107, 309
AsuHPI GGTGA 3 cut(s) 419, 515, 550
Bbv12I GWGCWC 1 cut(s) 341
BccI CCATC 4 cut(s) 20, 245, 322, 395
BciT130I CCWGG 1 cut(s) 132
BcoDI GTCTC 2 cut(s) 61, 187
BfuAI ACCTGC 1 cut(s) 348
BisI GCNGC 1 cut(s) 164
BlsI GCNGC 1 cut(s) 165
Bme1390I CCNGG 1 cut(s) 132
BmgT120I GGNCC 2 cut(s) 107, 309
BmiI GGNNCC 2 cut(s) 302, 310
BmrFI CCNGG 1 cut(s) 132
BmsI GCATC 3 cut(s) 182, 224, 526
BoxI GACNNNNGTC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 4 cut(s) 167, 177, 276, 304
BsaXI ACNNNNNCTCC 2 cut(s) 540, 570
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 1 cut(s) 124
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 4 cut(s) 167, 177, 276, 304
BseGI GGATG 5 cut(s) 149, 173, 256, 333, 339
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 124
Bsh1236I CGCG 2 cut(s) 75, 163
BshFI GGCC 4 cut(s) 109, 166, 194, 311
BsiHKAI GWGCWC 1 cut(s) 341
BsiSI CCGG 1 cut(s) 371
BslFI GGGAC 1 cut(s) 22
BsmAI GTCTC 2 cut(s) 61, 187
BsmFI GGGAC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 239
BsnI GGCC 4 cut(s) 109, 166, 194, 311
Bso31I GGTCTC 1 cut(s) 187
Bsp1286I GDGCHC 1 cut(s) 341
BspACI CCGC 2 cut(s) 163, 350
BspANI GGCC 4 cut(s) 109, 166, 194, 311
BspCNI CTCAG 1 cut(s) 211
BspFNI CGCG 2 cut(s) 75, 163
BspLI GGNNCC 2 cut(s) 302, 310
BspMI ACCTGC 1 cut(s) 348
BspTNI GGTCTC 1 cut(s) 187
BsrFI RCCGGY 1 cut(s) 370
BsrI ACTGG 1 cut(s) 124
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 4 cut(s) 167, 177, 276, 304
BssT1I CCWWGG 3 cut(s) 167, 177, 276
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 4 cut(s) 92, 205, 211, 523
Bst6I CTCTTC 1 cut(s) 378
BstAPI GCANNNNNTGC 2 cut(s) 243, 345
BstC8I GCNNGC 4 cut(s) 77, 293, 350, 372
BstDEI CTNAG 1 cut(s) 219
BstDSI CCRYGG 1 cut(s) 304
BstF5I GGATG 5 cut(s) 149, 173, 256, 333, 339
BstFNI CGCG 2 cut(s) 75, 163
BstMAI GTCTC 2 cut(s) 61, 187
BstMWI GCNNNNNNNGC 6 cut(s) 85, 243, 252, 292, 345, 354
BstNI CCWGG 1 cut(s) 132
BstPAI GACNNNNGTC 1 cut(s) 27
BstSCI CCNGG 1 cut(s) 130
BstUI CGCG 2 cut(s) 75, 163
BsuRI GGCC 4 cut(s) 109, 166, 194, 311
BtgI CCRYGG 1 cut(s) 304
BtsCI GGATG 5 cut(s) 149, 173, 256, 333, 339
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 3 cut(s) 216, 466, 519
BveI ACCTGC 1 cut(s) 348
Cac8I GCNNGC 4 cut(s) 77, 293, 350, 372
CaiI CAGNNNCTG 1 cut(s) 243
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 2 cut(s) 107, 309
CsiI ACCWGGT 1 cut(s) 130
CviAII CATG 1 cut(s) 196
CviJI RGCY 7 cut(s) 109, 127, 166, 194, 255, 275, 311
CviKI_1 RGCY 7 cut(s) 109, 127, 166, 194, 255, 275, 311
DdeI CTNAG 1 cut(s) 219
EaeI YGGCCR 1 cut(s) 164
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Eco130I CCWWGG 3 cut(s) 167, 177, 276
Eco31I GGTCTC 1 cut(s) 187
Eco32I GATATC 1 cut(s) 496
Eco57I CTGAAG 3 cut(s) 489, 525, 547
EcoO109I RGGNCCY 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 130
EcoRV GATATC 1 cut(s) 496
EcoT14I CCWWGG 3 cut(s) 167, 177, 276
ErhI CCWWGG 3 cut(s) 167, 177, 276
FaeI CATG 1 cut(s) 199
FaiI YATR 2 cut(s) 188, 197
FaqI GGGAC 1 cut(s) 22
FatI CATG 1 cut(s) 195
FauI CCCGC 1 cut(s) 357
Fnu4HI GCNGC 1 cut(s) 164
FokI GGATG 5 cut(s) 136, 160, 263, 340, 346
Fsp4HI GCNGC 1 cut(s) 164
GluI GCNGC 1 cut(s) 164
GsuI CTGGAG 1 cut(s) 141
HaeIII GGCC 4 cut(s) 109, 166, 194, 311
HapII CCGG 1 cut(s) 371
Hin1II CATG 1 cut(s) 199
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HinfI GANTC 4 cut(s) 47, 377, 476, 512
HpaI GTTAAC 1 cut(s) 451
HpaII CCGG 1 cut(s) 371
HphI GGTGA 3 cut(s) 419, 515, 550
Hpy166II GTNNAC 1 cut(s) 451
Hpy188III TCNNGA 2 cut(s) 440, 565
Hpy8I GTNNAC 1 cut(s) 451
Hpy99I CGWCG 2 cut(s) 26, 272
HpyAV CCTTC 3 cut(s) 218, 253, 571
HpyCH4III ACNGT 4 cut(s) 92, 205, 211, 523
HpyCH4V TGCA 4 cut(s) 88, 237, 291, 357
HpyF10VI GCNNNNNNNGC 6 cut(s) 85, 243, 252, 292, 345, 354
HpyF3I CTNAG 1 cut(s) 219
Hsp92II CATG 1 cut(s) 199
KroI GCCGGC 1 cut(s) 370
KroNI GCCGGC 1 cut(s) 372
KspAI GTTAAC 1 cut(s) 451
LpnPI CCDG 8 cut(s) 105, 117, 144, 283, 325, 343, 384, 550
LweI GCATC 3 cut(s) 182, 224, 526
MabI ACCWGGT 1 cut(s) 130
MaeIII GTNAC 3 cut(s) 205, 407, 556
MboII GAAGA 4 cut(s) 395, 401, 482, 503
MfeI CAATTG 1 cut(s) 32
MhlI GDGCHC 1 cut(s) 341
MluCI AATT 2 cut(s) 7, 32
MluI ACGCGT 1 cut(s) 73
MlyI GAGTC 2 cut(s) 386, 470
MmeI TCCRAC 2 cut(s) 90, 526
MnlI CCTC 8 cut(s) 98, 116, 163, 184, 214, 379, 481, 494
MroNI GCCGGC 1 cut(s) 370
MseI TTAA 2 cut(s) 420, 450
MspI CCGG 1 cut(s) 371
MspR9I CCNGG 1 cut(s) 132
MunI CAATTG 1 cut(s) 32
Mva1269I GAATGC 1 cut(s) 239
MvaI CCWGG 1 cut(s) 132
MvnI CGCG 2 cut(s) 75, 163
MwoI GCNNNNNNNGC 6 cut(s) 85, 243, 252, 292, 345, 354
NaeI GCCGGC 1 cut(s) 372
NgoMIV GCCGGC 1 cut(s) 370
NlaIII CATG 1 cut(s) 199
NlaIV GGNNCC 2 cut(s) 302, 310
NmuCI GTSAC 3 cut(s) 205, 407, 556
PctI GAATGC 1 cut(s) 239
PdiI GCCGGC 1 cut(s) 372
PfeI GAWTC 2 cut(s) 47, 512
PkrI GCNGC 1 cut(s) 165
PleI GAGTC 2 cut(s) 385, 470
PpsI GAGTC 2 cut(s) 385, 470
PshAI GACNNNNGTC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 2 cut(s) 302, 310
PspPI GGNCC 2 cut(s) 107, 309
PstNI CAGNNNCTG 1 cut(s) 243
SaqAI TTAA 2 cut(s) 420, 450
SatI GCNGC 1 cut(s) 164
Sau96I GGNCC 2 cut(s) 107, 309
SchI GAGTC 2 cut(s) 386, 470
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 1 cut(s) 341
SexAI ACCWGGT 1 cut(s) 130
SfaNI GCATC 3 cut(s) 182, 224, 526
Sse9I AATT 2 cut(s) 7, 32
SsiI CCGC 2 cut(s) 163, 350
SspI AATATT 2 cut(s) 84, 114
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 3 cut(s) 167, 177, 276
TaaI ACNGT 4 cut(s) 92, 205, 211, 523
TaqI TCGA 1 cut(s) 525
TasI AATT 2 cut(s) 7, 32
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 2 cut(s) 47, 512
Tru1I TTAA 2 cut(s) 420, 450
Tru9I TTAA 2 cut(s) 420, 450
TscAI CASTG 3 cut(s) 216, 466, 526
TseFI GTSAC 3 cut(s) 205, 407, 556
Tsp45I GTSAC 3 cut(s) 205, 407, 556
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 3 cut(s) 216, 466, 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.