FvH4_7g01800

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001291
Physical Location & Seq
Reverse (-)
2180757 .. 2183256
2500 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01800.t1

Sequence Viewer

Length: 2403 bp
ATGGCTCAAGCATACAATATCACGTATAGATCCAAAACCTCTTTCAATGTGTGTTACAACAAATCACGAACGCATGCGACCGGGCTATGGAATGCTGAAAACGCCTGGGTTGCATCTCTCCCACTGCTCATCATCCAACTCCTCTCCATCTTGTTGCTCAGTCACATTCTTGCCTACCTCCTCAAACCCTTGCGCCAACCTCGCATTGTTGCTAATATTCTGGCTGGAGTTCTGTTAGGTCCATCTGTGTTTTCGATCCCGAAGATTGTGGGAGTGACCATAACTCCTTTTGATAGCATAATCACCTTAGAGACTATGGCAAACTTGGGGCTTGTGTACCATGTTTTCATGATCGGGTTAGACTTCGAAATCAGACCAGTAGTACGTGCAGGGACAAAGGCTATCAGCATTGCCGTCGCCGGAGTAGCCTTTGCCATCCCCGCCGGGTGGCTCTTGCATACCTATGTCCTCTTCAGAGACTTCGAAACTGTGAAAGACAAAAGCAATTTGAAAAACGTAGCCTATGGCCCCATCTTCTGGGGAATTATCCTCGCCGGCACCAACTTCGCTGATCTCACCCAAATTCTTGCCGACTTCAAGCTTCTCCACTCGGAGGTGGGAAGACTAGCCTTATCCGGTGCCATTATCTCTGACGTGTCATCTTGGTTTCTTTTTCTTGTGGTGATGGCTATACTCGGCGATGGTAAATTGTACACGGTGTTGTTAACCTTTGGCTTCATTGCATTCTGTGTCTTCATACTTAGGCCAGCTCTGGCCAGGGCAATGTGTTACACCACGAAAGATGACAGCTATAGTGATGCCCAAATATGTTGTATTCTGTTTGGGATTGTATTGTGCGGATTGATAACCGATGCCTGCGGTGGACATTCCATCGTCGGACCTTTCATGTTGGGAGCAATTATGCCTAAGGGGATGCACTTCAAACAAATGTTCAAAGATCGTGTTGGAAAGTTTGTGAAAAGCCTTTTCATGCCCCTCTTTTACTTGGTCATCGGGGTGAGATTCAATTCCTTCTGGCTTTTTGGTGATTTCAATCAGCAGCTGAAAACTGAATCAAACGTGGCTCAGCTTGTTGGGGTGATCATCCTGGCTTTCGGAGCGAAGAGTGTCGGCACTTTCATCGCTGCTTTGTTCAACAAAATGACAATTCGAGATAGTTTAGTTCTGGGAATCCTCATGAACACTAAAGGCTTATTGGCACTCATTATACTCAGCTCCTCTCTTGACCTAAAGGCTTTGGACAACCAAACATATGGAGTAATGTTGGTTGCAGTTTGGGCAATGACGGTTCCGGTCGGACCTTTCCTAGCCCTCTTCTGCAAGTCCACCAAGAGTTCTACACTAAATCAACATAGAAGCATACAAGGTGTAGGAGCTGAAACAGAGCTTCGAATCCTTGCATGCACCCACACTGCGAGCAATGCATCCGGCATCATAAGCCTCATCGAGGCCTCCAATCCGTCCAGCAAATCCCCAATTCATGTCTTAGCTGTCAAACTGGTCGAACTTACTGACCATAACGCTGCCATGCTAATCGTACATGATGCTTGCCACAAAACCGGAGCAGATGGCAACAAGCTACAGTCTGACTTCAGCTGCAATGCATTTGAGAACTATGCAAGGAAAAGAGAGAATGTCTCTGTCCAGTCACTCACTGCTGTGTCTGCCTACTCTACTATGCACGAGGATATATGCAACATGGCTAACGAGAAGGATGTCAACCTCATAATCATCCCGTTCCGAATGCAAACTACCATCGATGGAGTCCCAGATGATCGTTATGATCCTCTTCTTGCAGAAATCAGTAAGAAGGTGACGGAAAATGCAAAGTGCTCTGCAGGTCTTTTTGTTGATTCTGGACTTGTCACATCCAGAATTACAGACTTCCAAAATGCTTCTATGGAGTCCCACAAATACGCCATGTTTTTCATTGGAGGGCCAGATGATCGAGAGGCACTAGCGTACGCATGGAGGATGGCAGGGCATCCTAAGGTAAACTTAACAGTTATACGGTTCACTCCAAGTAAAGAGGCATGGGAGGAGAGCAGTCATAGCGGTAAGGAACACACGGCATATGAAAACATTAGGCAGCAGAAACAACTTGACGACGCATGCATAGATGAGTTCAAACTGAAGTCCATGAATGAACCGTCTATAGTTCTGTGTGAGAAGCTGGCCAATAATCTGGAAGAAATTATCAATATAGTAAGCACCATGGAAGGTCAATATCAGCTGTACATAGTGGGAAGAGGTCATGATGGTTGCTCTCCAATAACACTACCATCGTTCGAATCCATCAGGACCAATAAGCTTGGAACTTTGGGGGAGACGTTGGCATCTTCGAGCTTACTGGGAAGTACATCCCTTCTTATAATGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

801

Amino Acids

87.67

Weight (kDa)

7.55

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 54 - 442 1.4e-47 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 502 - 629 5.7e-08 Plant cation/H(+) antiporter domain
CHX17_C PF23259 648 - 800 1.6e-10 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2393
Acc36I ACCTGC 1 cut(s) 1850
AccB1I GGYRCC 2 cut(s) 557, 638
AccB7I CCANNNNNTGG 1 cut(s) 537
AciI CCGC 4 cut(s) 441, 858, 879, 2076
AclWI GGATC 3 cut(s) 24, 250, 1799
AcoI YGGCCR 2 cut(s) 774, 2196
AcsI RAATTY 1 cut(s) 582
AcuI CTGAAG 3 cut(s) 457, 1597, 2174
AfaI GTAC 7 cut(s) 338, 384, 713, 1560, 1985, 2258, 2380
AfiI CCNNNNNNNGG 5 cut(s) 87, 447, 537, 613, 1468
AflIII ACRYGT 1 cut(s) 654
AgsI TTSAA 9 cut(s) 46, 511, 598, 943, 955, 1027, 1054, 1156, 2149
AjiI CACGTC 1 cut(s) 655
AjnI CCWGG 3 cut(s) 104, 776, 1107
AjuI GAANNNNNNNTTGG 2 cut(s) 26, 58
AleI CACNNNNGTG 1 cut(s) 1679
Alw21I GWGCWC 1 cut(s) 1856
Alw26I GTCTC 4 cut(s) 305, 471, 1663, 2342
AlwI GGATC 3 cut(s) 24, 250, 1799
AlwNI CAGNNNCTG 1 cut(s) 1063
AoxI GGCC 6 cut(s) 526, 764, 774, 1470, 1958, 2196
ApeKI GCWGC 5 cut(s) 1060, 1145, 1544, 1617, 2110
ApoI RAATTY 1 cut(s) 582
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 6 cut(s) 239, 527, 899, 1319, 1958, 2322
AsuC2I CCSGG 2 cut(s) 82, 445
AsuHPI GGTGA 7 cut(s) 295, 568, 694, 1030, 1058, 1111, 1846
AsuII TTCGAA 4 cut(s) 366, 483, 1411, 2310
AvaII GGWCC 4 cut(s) 239, 899, 1319, 2322
AxyI CCTNAGG 2 cut(s) 927, 2010
BalI TGGCCA 2 cut(s) 776, 2198
BanI GGYRCC 2 cut(s) 557, 638
BauI CACGAG 1 cut(s) 1703
BbsI GAAGAC 2 cut(s) 628, 745
Bbv12I GWGCWC 1 cut(s) 1856
BbvI GCAGC 5 cut(s) 1072, 1132, 1531, 1604, 2122
BceAI ACGGC 2 cut(s) 398, 2106
BcgI CGANNNNNNTGC 6 cut(s) 397, 431, 547, 581, 1123, 1157
BciT130I CCWGG 3 cut(s) 106, 778, 1109
BclI TGATCA 1 cut(s) 1101
BcnI CCSGG 2 cut(s) 82, 445
BcoDI GTCTC 4 cut(s) 305, 471, 1663, 2342
BfaI CTAG 3 cut(s) 626, 1328, 1979
BfmI CTRYAG 4 cut(s) 811, 1601, 1857, 2175
BfuAI ACCTGC 1 cut(s) 1850
BisI GCNGC 5 cut(s) 1061, 1146, 1545, 1618, 2111
BlpI GCTNAGC 1 cut(s) 1086
BlsI GCNGC 5 cut(s) 1062, 1147, 1546, 1619, 2112
Bme1390I CCNGG 5 cut(s) 82, 106, 445, 778, 1109
Bme18I GGWCC 4 cut(s) 239, 899, 1319, 2322
BmgBI CACGTC 1 cut(s) 655
BmgT120I GGNCC 6 cut(s) 239, 527, 899, 1319, 1958, 2322
BmiI GGNNCC 4 cut(s) 529, 559, 640, 1311
BmrFI CCNGG 5 cut(s) 82, 106, 445, 778, 1109
BmrI ACTGGG 1 cut(s) 2381
BmsI GCATC 9 cut(s) 122, 808, 862, 924, 1454, 1461, 1555, 2014, 2366
BmuI ACTGGG 1 cut(s) 2381
BpiI GAAGAC 2 cut(s) 628, 745
BplI GAGNNNNNCTC 2 cut(s) 1643, 1675
BpmI CTGGAG 1 cut(s) 246
Bpu1102I GCTNAGC 1 cut(s) 1086
Bpu14I TTCGAA 4 cut(s) 366, 483, 1411, 2310
BpuMI CCSGG 2 cut(s) 82, 445
Bsa29I ATCGAT 1 cut(s) 1779
BsaAI YACGTR 2 cut(s) 24, 386
BsaBI GATNNNNATC 1 cut(s) 1053
BsaJI CCNNGG 3 cut(s) 105, 777, 2235
BsaWI WCCGGW 3 cut(s) 635, 1312, 1580
BsaXI ACNNNNNCTCC 2 cut(s) 268, 298
Bsc4I CCNNNNNNNGG 5 cut(s) 87, 447, 537, 613, 1468
Bse118I RCCGGY 1 cut(s) 554
Bse1I ACTGG 4 cut(s) 377, 1524, 1666, 2376
Bse21I CCTNAGG 2 cut(s) 927, 2010
Bse3DI GCAATG 6 cut(s) 408, 738, 789, 1308, 1447, 1627
Bse8I GATNNNNATC 1 cut(s) 1053
BseBI CCWGG 3 cut(s) 106, 778, 1109
BseCI ATCGAT 1 cut(s) 1779
BseDI CCNNGG 3 cut(s) 105, 777, 2235
BseJI GATNNNNATC 1 cut(s) 1053
BseLI CCNNNNNNNGG 5 cut(s) 87, 447, 537, 613, 1468
BseMI GCAATG 6 cut(s) 408, 738, 789, 1308, 1447, 1627
BseMII CTCAG 3 cut(s) 172, 1100, 1246
BseNI ACTGG 4 cut(s) 377, 1524, 1666, 2376
BseRI GAGGAG 4 cut(s) 131, 170, 1228, 2075
BseXI GCAGC 5 cut(s) 1072, 1132, 1531, 1604, 2122
BsgI GTGCAG 1 cut(s) 408
Bsh1285I CGRYCG 2 cut(s) 81, 1317
BshFI GGCC 6 cut(s) 528, 766, 776, 1472, 1960, 2198
BshNI GGYRCC 2 cut(s) 557, 638
BshVI ATCGAT 1 cut(s) 1779
BsiEI CGRYCG 2 cut(s) 81, 1317
BsiHKAI GWGCWC 1 cut(s) 1856
BsiSI CCGG 8 cut(s) 81, 420, 444, 555, 636, 1313, 1449, 1581
BsiWI CGTACG 1 cut(s) 1983
BslFI GGGAC 3 cut(s) 406, 1772, 1912
BslI CCNNNNNNNGG 5 cut(s) 87, 447, 537, 613, 1468
BsmAI GTCTC 4 cut(s) 305, 471, 1663, 2342
BsmBI CGTCTC 1 cut(s) 2342
BsmFI GGGAC 3 cut(s) 406, 1772, 1912
BsmI GAATGC 3 cut(s) 97, 743, 1770
BsnI GGCC 6 cut(s) 528, 766, 776, 1472, 1960, 2198
Bsp119I TTCGAA 4 cut(s) 366, 483, 1411, 2310
Bsp1286I GDGCHC 1 cut(s) 1856
Bsp1407I TGTACA 2 cut(s) 711, 2256
Bsp143I GATC 9 cut(s) 29, 255, 351, 571, 958, 1101, 1795, 1804, 1966
Bsp1720I GCTNAGC 1 cut(s) 1086
Bsp19I CCATGG 1 cut(s) 2235
BspACI CCGC 4 cut(s) 441, 858, 879, 2076
BspANI GGCC 6 cut(s) 528, 766, 776, 1472, 1960, 2198
BspCNI CTCAG 3 cut(s) 171, 1099, 1245
BspDI ATCGAT 1 cut(s) 1779
BspHI TCATGA 3 cut(s) 348, 1197, 2275
BspLI GGNNCC 4 cut(s) 529, 559, 640, 1311
BspMAI CTGCAG 1 cut(s) 1861
BspMI ACCTGC 1 cut(s) 1850
BspPI GGATC 3 cut(s) 24, 250, 1799
BspT104I TTCGAA 4 cut(s) 366, 483, 1411, 2310
BspT107I GGYRCC 2 cut(s) 557, 638
BsrDI GCAATG 6 cut(s) 408, 738, 789, 1308, 1447, 1627
BsrFI RCCGGY 1 cut(s) 554
BsrGI TGTACA 2 cut(s) 711, 2256
BsrI ACTGG 4 cut(s) 377, 1524, 1666, 2376
BssAI RCCGGY 1 cut(s) 554
BssECI CCNNGG 3 cut(s) 105, 777, 2235
BssMI GATC 9 cut(s) 29, 255, 351, 571, 958, 1101, 1795, 1804, 1966
BssSI CACGAG 1 cut(s) 1703
BssT1I CCWWGG 1 cut(s) 2235
Bst2BI CACGAG 1 cut(s) 1703
Bst2UI CCWGG 3 cut(s) 106, 778, 1109
Bst4CI ACNGT 7 cut(s) 490, 718, 1309, 1605, 2026, 2034, 2172
Bst6I CTCTTC 5 cut(s) 476, 1118, 1340, 1815, 2263
BstAUI TGTACA 2 cut(s) 711, 2256
BstBAI YACGTR 2 cut(s) 24, 386
BstBI TTCGAA 4 cut(s) 366, 483, 1411, 2310
BstC8I GCNNGC 9 cut(s) 75, 556, 768, 877, 1423, 1438, 1570, 2134, 2196
BstDEI CTNAG 8 cut(s) 158, 307, 761, 927, 1086, 1232, 1507, 2010
BstDSI CCRYGG 1 cut(s) 2235
BstENI CCTNNNNNAGG 1 cut(s) 1466
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 9 cut(s) 32, 258, 354, 574, 961, 1104, 1798, 1807, 1969
BstMAI GTCTC 4 cut(s) 305, 471, 1663, 2342
BstMBI GATC 9 cut(s) 29, 255, 351, 571, 958, 1101, 1795, 1804, 1966
BstMCI CGRYCG 2 cut(s) 81, 1317
BstNI CCWGG 3 cut(s) 106, 778, 1109
BstNSI RCATGY 3 cut(s) 77, 1425, 2136
BstSCI CCNGG 5 cut(s) 80, 104, 443, 776, 1107
BstSFI CTRYAG 4 cut(s) 811, 1601, 1857, 2175
BstV1I GCAGC 5 cut(s) 1072, 1132, 1531, 1604, 2122
BstV2I GAAGAC 2 cut(s) 628, 745
BstX2I RGATCY 1 cut(s) 29
BstXI CCANNNNNNTGG 2 cut(s) 1274, 2206
BstYI RGATCY 1 cut(s) 29
Bsu15I ATCGAT 1 cut(s) 1779
Bsu36I CCTNAGG 2 cut(s) 927, 2010
BsuRI GGCC 6 cut(s) 528, 766, 776, 1472, 1960, 2198
BsuTUI ATCGAT 1 cut(s) 1779
BtgI CCRYGG 1 cut(s) 2235
BtgZI GCGATG 2 cut(s) 714, 1126
BtrI CACGTC 1 cut(s) 655
BtsI GCAGTG 3 cut(s) 122, 1431, 1674
BtsIMutI CAGTG 3 cut(s) 122, 1431, 1674
BveI ACCTGC 1 cut(s) 1850
Cac8I GCNNGC 9 cut(s) 75, 556, 768, 877, 1423, 1438, 1570, 2134, 2196
CaiI CAGNNNCTG 1 cut(s) 1063
CciI TCATGA 3 cut(s) 348, 1197, 2275
CfoI GCGC 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 554
Cfr13I GGNCC 6 cut(s) 239, 527, 899, 1319, 1958, 2322
ClaI ATCGAT 1 cut(s) 1779
CseI GACGC 1 cut(s) 2138
Csp6I GTAC 7 cut(s) 337, 383, 712, 1559, 1984, 2257, 2379
CviQI GTAC 7 cut(s) 337, 383, 712, 1559, 1984, 2257, 2379
DdeI CTNAG 8 cut(s) 158, 307, 761, 927, 1086, 1232, 1507, 2010
DpnI GATC 9 cut(s) 31, 257, 353, 573, 960, 1103, 1797, 1806, 1968
DpnII GATC 9 cut(s) 29, 255, 351, 571, 958, 1101, 1795, 1804, 1966
EaeI YGGCCR 2 cut(s) 774, 2196
Eam1104I CTCTTC 5 cut(s) 476, 1118, 1340, 1815, 2263
EarI CTCTTC 5 cut(s) 476, 1118, 1340, 1815, 2263
Eco130I CCWWGG 1 cut(s) 2235
Eco147I AGGCCT 1 cut(s) 1472
Eco47I GGWCC 4 cut(s) 239, 899, 1319, 2322
Eco57I CTGAAG 3 cut(s) 457, 1597, 2174
Eco81I CCTNAGG 2 cut(s) 927, 2010
EcoNI CCTNNNNNAGG 1 cut(s) 1466
EcoRII CCWGG 3 cut(s) 104, 776, 1107
EcoT14I CCWWGG 1 cut(s) 2235
EcoT22I ATGCAT 3 cut(s) 1447, 1627, 2138
ErhI CCWWGG 1 cut(s) 2235
Esp3I CGTCTC 1 cut(s) 2342
FalI AAGNNNNNCTT 2 cut(s) 2003, 2035
FaqI GGGAC 3 cut(s) 406, 1772, 1912
FauI CCCGC 1 cut(s) 448
FauNDI CATATG 2 cut(s) 1273, 2095
FbaI TGATCA 1 cut(s) 1101
Fnu4HI GCNGC 5 cut(s) 1061, 1146, 1545, 1618, 2111
Fsp4HI GCNGC 5 cut(s) 1061, 1146, 1545, 1618, 2111
FspBI CTAG 3 cut(s) 626, 1328, 1979
GlaI GCGC 1 cut(s) 194
GluI GCNGC 5 cut(s) 1061, 1146, 1545, 1618, 2111
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 6 cut(s) 528, 766, 776, 1472, 1960, 2198
HapII CCGG 8 cut(s) 81, 420, 444, 555, 636, 1313, 1449, 1581
HgaI GACGC 1 cut(s) 2138
HhaI GCGC 1 cut(s) 195
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HincII GTYRAC 2 cut(s) 726, 1741
HindII GTYRAC 2 cut(s) 726, 1741
HindIII AAGCTT 2 cut(s) 599, 2330
HinfI GANTC 8 cut(s) 1023, 1073, 1191, 1413, 1785, 1874, 1925, 2312
HpaI GTTAAC 1 cut(s) 726
HpaII CCGG 8 cut(s) 81, 420, 444, 555, 636, 1313, 1449, 1581
HphI GGTGA 7 cut(s) 295, 568, 694, 1030, 1058, 1111, 1846
Hpy166II GTNNAC 8 cut(s) 337, 714, 726, 884, 1347, 1741, 2017, 2037
Hpy188I TCNGA 9 cut(s) 374, 476, 613, 652, 899, 1118, 1319, 1609, 1763
Hpy8I GTNNAC 8 cut(s) 337, 714, 726, 884, 1347, 1741, 2017, 2037
Hpy99I CGWCG 3 cut(s) 419, 899, 2132
HpyAV CCTTC 5 cut(s) 1042, 1726, 1825, 2234, 2396
HpyCH4III ACNGT 7 cut(s) 490, 718, 1309, 1605, 2026, 2034, 2172
HpyCH4IV ACGT 6 cut(s) 23, 385, 516, 654, 1080, 2351
HpyF3I CTNAG 8 cut(s) 158, 307, 761, 927, 1086, 1232, 1507, 2010
HpySE526I ACGT 6 cut(s) 23, 385, 516, 654, 1080, 2351
HspAI GCGC 1 cut(s) 193
KroI GCCGGC 1 cut(s) 554
KroNI GCCGGC 1 cut(s) 556
Ksp22I TGATCA 1 cut(s) 1101
KspAI GTTAAC 1 cut(s) 726
Kzo9I GATC 9 cut(s) 29, 255, 351, 571, 958, 1101, 1795, 1804, 1966
LmnI GCTCC 5 cut(s) 914, 1118, 1241, 1394, 1583
Lsp1109I GCAGC 5 cut(s) 1072, 1132, 1531, 1604, 2122
LweI GCATC 9 cut(s) 122, 808, 862, 924, 1454, 1461, 1555, 2014, 2366
MaeI CTAG 3 cut(s) 626, 1328, 1979
MaeII ACGT 6 cut(s) 23, 385, 516, 654, 1080, 2351
MaeIII GTNAC 7 cut(s) 53, 161, 274, 788, 1668, 1834, 1885
MalI GATC 9 cut(s) 31, 257, 353, 573, 960, 1103, 1797, 1806, 1968
MboI GATC 9 cut(s) 29, 255, 351, 571, 958, 1101, 1795, 1804, 1966
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 1 cut(s) 1856
MlsI TGGCCA 2 cut(s) 776, 2198
MluNI TGGCCA 2 cut(s) 776, 2198
MlyI GAGTC 2 cut(s) 1794, 1934
MmeI TCCRAC 4 cut(s) 160, 877, 946, 1297
Mox20I TGGCCA 2 cut(s) 776, 2198
Mph1103I ATGCAT 3 cut(s) 1447, 1627, 2138
MroNI GCCGGC 1 cut(s) 554
MscI TGGCCA 2 cut(s) 776, 2198
MseI TTAA 2 cut(s) 725, 2021
MslI CAYNNNNRTG 3 cut(s) 462, 1016, 1679
Msp20I TGGCCA 2 cut(s) 776, 2198
MspA1I CMGCKG 3 cut(s) 1063, 1617, 2254
MspI CCGG 8 cut(s) 81, 420, 444, 555, 636, 1313, 1449, 1581
MspR9I CCNGG 5 cut(s) 82, 106, 445, 778, 1109
Mva1269I GAATGC 3 cut(s) 97, 743, 1770
MvaI CCWGG 3 cut(s) 106, 778, 1109
NaeI GCCGGC 1 cut(s) 556
NciI CCSGG 2 cut(s) 82, 445
NcoI CCATGG 1 cut(s) 2235
NdeI CATATG 2 cut(s) 1273, 2095
NdeII GATC 9 cut(s) 29, 255, 351, 571, 958, 1101, 1795, 1804, 1966
NgoMIV GCCGGC 1 cut(s) 554
NlaIV GGNNCC 4 cut(s) 529, 559, 640, 1311
NmeAIII GCCGAG 1 cut(s) 675
NmuCI GTSAC 5 cut(s) 161, 274, 1668, 1834, 1885
NsiI ATGCAT 3 cut(s) 1447, 1627, 2138
NspI RCATGY 3 cut(s) 77, 1425, 2136
NspV TTCGAA 4 cut(s) 366, 483, 1411, 2310
OliI CACNNNNGTG 1 cut(s) 1679
PaeI GCATGC 3 cut(s) 77, 1425, 2136
PagI TCATGA 3 cut(s) 348, 1197, 2275
PceI AGGCCT 1 cut(s) 1472
PctI GAATGC 3 cut(s) 97, 743, 1770
PdiI GCCGGC 1 cut(s) 556
PfeI GAWTC 6 cut(s) 1023, 1073, 1191, 1413, 1874, 2312
Pfl23II CGTACG 1 cut(s) 1983
PflMI CCANNNNNTGG 1 cut(s) 537
PkrI GCNGC 5 cut(s) 1062, 1147, 1546, 1619, 2112
PleI GAGTC 2 cut(s) 1793, 1933
PpsI GAGTC 2 cut(s) 1793, 1933
Ppu21I YACGTR 2 cut(s) 24, 386
PsiI TTATAA 1 cut(s) 2393
Psp6I CCWGG 3 cut(s) 104, 776, 1107
PspGI CCWGG 3 cut(s) 104, 776, 1107
PspLI CGTACG 1 cut(s) 1983
PspN4I GGNNCC 4 cut(s) 529, 559, 640, 1311
PspPI GGNCC 6 cut(s) 239, 527, 899, 1319, 1958, 2322
PstI CTGCAG 1 cut(s) 1861
PstNI CAGNNNCTG 1 cut(s) 1063
PsuI RGATCY 1 cut(s) 29
PvuII CAGCTG 3 cut(s) 1063, 1617, 2254
RsaI GTAC 7 cut(s) 338, 384, 713, 1560, 1985, 2258, 2380
RsaNI GTAC 7 cut(s) 337, 383, 712, 1559, 1984, 2257, 2379
RseI CAYNNNNRTG 3 cut(s) 462, 1016, 1679
SaqAI TTAA 2 cut(s) 725, 2021
SatI GCNGC 5 cut(s) 1061, 1146, 1545, 1618, 2111
Sau3AI GATC 9 cut(s) 29, 255, 351, 571, 958, 1101, 1795, 1804, 1966
Sau96I GGNCC 6 cut(s) 239, 527, 899, 1319, 1958, 2322
SchI GAGTC 2 cut(s) 1794, 1934
ScrFI CCNGG 5 cut(s) 82, 106, 445, 778, 1109
SduI GDGCHC 1 cut(s) 1856
SfaNI GCATC 9 cut(s) 122, 808, 862, 924, 1454, 1461, 1555, 2014, 2366
SfcI CTRYAG 4 cut(s) 811, 1601, 1857, 2175
SfuI TTCGAA 4 cut(s) 366, 483, 1411, 2310
SinI GGWCC 4 cut(s) 239, 899, 1319, 2322
SmiMI CAYNNNNRTG 3 cut(s) 462, 1016, 1679
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
SphI GCATGC 3 cut(s) 77, 1425, 2136
SseBI AGGCCT 1 cut(s) 1472
SsiI CCGC 4 cut(s) 441, 858, 879, 2076
SspI AATATT 1 cut(s) 217
SspMI CTAG 3 cut(s) 626, 1328, 1979
StuI AGGCCT 1 cut(s) 1472
StyD4I CCNGG 5 cut(s) 80, 104, 443, 776, 1107
StyI CCWWGG 1 cut(s) 2235
TaaI ACNGT 7 cut(s) 490, 718, 1309, 1605, 2026, 2034, 2172
TaiI ACGT 6 cut(s) 26, 388, 519, 657, 1083, 2354
TatI WGTACW 3 cut(s) 711, 2256, 2378
TfiI GAWTC 6 cut(s) 1023, 1073, 1191, 1413, 1874, 2312
Tru1I TTAA 2 cut(s) 725, 2021
Tru9I TTAA 2 cut(s) 725, 2021
TscAI CASTG 3 cut(s) 129, 1438, 1681
TseFI GTSAC 5 cut(s) 161, 274, 1668, 1834, 1885
TseI GCWGC 5 cut(s) 1060, 1145, 1544, 1617, 2110
Tsp45I GTSAC 5 cut(s) 161, 274, 1668, 1834, 1885
TspGWI ACGGA 2 cut(s) 1470, 1853
TspRI CASTG 3 cut(s) 129, 1438, 1681
Van91I CCANNNNNTGG 1 cut(s) 537
VpaK11BI GGWCC 4 cut(s) 239, 899, 1319, 2322
XagI CCTNNNNNAGG 1 cut(s) 1466
XapI RAATTY 1 cut(s) 582
XceI RCATGY 3 cut(s) 77, 1425, 2136
XspI CTAG 3 cut(s) 626, 1328, 1979
Zsp2I ATGCAT 3 cut(s) 1447, 1627, 2138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.