Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001291

Physical Location & Seq

Genomic Coordinates Reverse (-)

34216563 .. 34219015

Gene Structure 2453 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1285100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2289 bp

ATGAATTTTCAGGCTGGAATGACAACGAGTACGAGGGCTTTGGGTCAACTGACGAAACCTATATTCCCATTTAGTACCTTGAAGATTACTGAGACCATGGGAAACTTGGCCCTCGTTTACTATATGTTTCTAGTGGGTTTAGAGCTTGATTTTAAACCAGTAGTTCGTGCTGGGAAGAAGGCTCTCAGCATTGCGCTCACTGGCTTTTTCTTTTCCGTCCTACTTGGTTGGGTCTTGTGTCGTTATTTACTCCTCCAAGATTTTGTCGCACAAACACATGATGGAGATCAGGAACGTCCCACTATAAACGGCCCATTGTTCTGGGGCGTTGCTCTCGCCACTACCAACTTCCCAGACCTTGCCAGAATCCTCGCGGACCTCAAACTTCTTTACTCTGATGTTGGAGGACTTGCGTTATCCGCCTCTGTTATCACCGACTTGTGCTCCTGGATTCTTCTTTTGCTCGTAATGGCCATAATCAACAGCGTCCAGATGTATGCAGTGGGCTTGACCTCGGTTTTTCTAGGACTCTGCGTTTTTGTAGTACGTCCTGCTCTGTCATGGATTGTTCACCGAGTACGCGAAAGAGAAAAAGAGGAGAGGAACAACAGTCAGCTTATACGCTTTGTTTTAGCTGGGGTGTTGCTCTCTGGGGTCATTACCGATGCCTGTGGATCCCATTCCATTGTAGGGCCTTTTGTGTTAGGAGCCATTATGCCTAAGGGCGAGTTTACGGACATGCTTAAACAGAAGGTGGGAAATTTTGTGCCTTCGATTCTGATGCCTCTTTACTTCTGTATCAATGGAGGAAGAGTCAACTTTGAGGATATTCTCGGTGACAGAAAAAATCCAATAGAGCAAAAAACTGGGGCTACCGTCGGTCGTGTAGTGTGGGTTACCATCATTGCTTTCGCAACTAAAACTGTGAGCACTTTCGTTGCCGGCATAATCAACAAAATGTCACCCCGGGATAGTTTGGCTCTTGGAGTGCTCATGAACACCAAAGGGTTATTGACACTCATTATACTCAATAGTGGCCGGGACATAAAGGCATTGAACAAACAAACGTTCGGAGTATTGATGGTGGTGATTTGGGTAATGACATTTGCGGTTGGCCCGTTTATAGCCTACTTTTACAAGTCCAGTGCCAAGACTTTCGTGCAGTACAAACAGAGGAGTTTAGCAAGCGTAGGACCTAACAACCAGTTTCGAGTCCTTGTATGCGTTCACACCTCACGCAATGTGCCGGGCATTATCAACCTCCTCGAGGCTTCTAATCCAACAACACAATCCCCTCTTCATGTTCTTGCTGTCCACCTTGTGGAACTAACTGGACACGCTTCGGCCATGTTGGTAGTGCACGATGCTTGCAAGACCAAAGATGTAAACTTTCCCGACAAATCTTCGTCTCCGACAAATGCCATCGAGTTATATGCCAAACAAAGGCAAAGTGTCACAGTGCAATCACTCACGGCAGTGTCTGCTTATCCGACGATGCATGAGGACATATGTAACCTGGCTGAGGACAATCGTGTCTCCATAATAATTATCCCGTATCATAAGAAAGCTACAATATTGGATGGAGAAGGAGGGGCAAGAGATGAAGACAAATCGCATTTAAAGAACCTCAACAACAACTTGATGGAAAATGCGCGGTGCTCCGTGGGTGTTCTTTTGGATCGGGGCATTGGTACATCCAACTACTTTGACTGCTGCCGCCACTTTACCATGCTCTTCTTTGGAGGGGCAGATGACCGCGAGGCATTAGCGTATGCGGGGAGGATGGCTGGACATCCAAGGGTTACCCTAACTGTTATAACGTTTAACATCATGAGTAAAGAGGCAGCAAAAACGTGCGTCGATGATGATGATGACGGTGATGAGTATAATGACGAGGACGAGGACGACAATGAGAACGACAACGACGACGACTACGACGATGTTACTCTTGAGGGAATGAAAAGTACTGGAGAAGAGAAAAAAATGGATGACTTGTACTTGGATGAGTTTAGGTTGAGGTCAATGAATGATGCCTCCATAAAATTTGTAGAGAATTGGGTGACCAGTTGGGAACAAATTCTCTCATTAATACAATCCACGGAGGGTGAATATGATATGTTTATAGTGGGTAGAAGACATGGAGAAATGCAAGAGGTCGCCACAACGTTACTGGATGACGATGACTCTAACGACATTGGAGTTCTTGGAGAGGCATTGGTATCTTCAATCTTCACAGCTAGTACATCCATTCTAATTGTGCAAAAAAGTGAATTTGCTCATGATTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

763

Amino Acids

84.08

Weight (kDa)

5.19

Isoelectric Point (pI)

32.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	23 - 372	7.2e-45	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	434 - 566	5.3e-16	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	576 - 608	2e-07	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001291)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g25710 FvH4_5g31470 FvH4_7g01800
malus_domestica	MD02G1284400.v1.1 MD02G1284600.v1.1 MD02G1284900.v1.1 MD02G1285000.v1.1 MD02G1285100.v1.1 MD02G1296900.v1.1 MD07G1050200.v1.1 MD07G1050300.v1.1 MD15G1139600.v1.1
prunus_persica	Prupe.2G027500_v2.0.a1 Prupe.2G038300_v2.0.a1 Prupe.2G040900_v2.0.a1 Prupe.4G283300_v2.0.a1 Prupe.4G283400_v2.0.a1 Prupe.4G283500_v2.0.a1 Prupe.4G283500_v2.0.a1 Prupe.4G284600_v2.0.a1 Prupe.4G284900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0318411 RchiOBHm_Chr5g0030741 RchiOBHm_Chr7g0226891
rosa_laevigata	RLG00000001685 RLG00000030540 RLG00000033248
rosa_multiflora	Rmu_co8328645.1_g000001 Rmu_co8411059.1_g000001 Rmu_sc0002662.1_g000016 Rmu_sc0003238.1_g000035 Rmu_sc0003936.1_g000039 Rmu_sc0034904.1_g000001
rosa_roxburghii	Rroxscaffold_3G00232440 Rroxscaffold_4G00329260
rosa_rugosa	Rorug01G0021100 Rorug05G0120500 Rorug07G0240000.1
rosa_samantha	Rh1AG033200 Rh1BG029000 Rh1CG031500 Rh1DG038000 Rh5AG212000 Rh5BG211100 Rh5CG234800 Rh5DG216300 Rh7AG387600 Rh7BG374100 Rh7CG407200 Rh7DG386300
rosa_wichuraiana	Rw0G000940 Rw0G000950 Rw1G002660 Rw5G019460 Rw7G032560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1817
AasI	GACNNNNNNGTC	1 cut(s)	1532
AbsI	CCTCGAGG	1 cut(s)	1265
AccB7I	CCANNNNNTGG	1 cut(s)	1321
AccII	CGCG	4 cut(s)	374, 582, 1654, 1758
AciI	CCGC	7 cut(s)	374, 420, 1109, 1654, 1717, 1756, 1775
AclI	AACGTT	3 cut(s)	1067, 1820, 2165
AclWI	GGATC	3 cut(s)	669, 682, 1686
AcoI	YGGCCR	3 cut(s)	471, 1036, 1344
AcsI	RAATTY	5 cut(s)	4, 760, 2042, 2076, 2270
AdeI	CACNNNGTG	1 cut(s)	1321
AfaI	GTAC	9 cut(s)	31, 76, 546, 579, 1166, 1693, 1966, 1997, 2242
AfiI	CCNNNNNNNGG	5 cut(s)	690, 1267, 1321, 1443, 1522
AgsI	TTSAA	3 cut(s)	82, 1057, 2226
AjnI	CCWGG	2 cut(s)	446, 1515
AleI	CACNNNNGTG	1 cut(s)	1475
AluBI	AGCT	5 cut(s)	145, 616, 635, 1568, 2237
AluI	AGCT	5 cut(s)	145, 616, 635, 1568, 2237
Alw21I	GWGCWC	5 cut(s)	446, 932, 993, 1362, 1661
Alw26I	GTCTC	3 cut(s)	86, 1413, 1540
Alw44I	GTGCAC	1 cut(s)	1358
AlwI	GGATC	3 cut(s)	669, 682, 1686
AlwNI	CAGNNNCTG	1 cut(s)	1481
Ama87I	CYCGRG	2 cut(s)	966, 1265
AoxI	GGCC	7 cut(s)	108, 310, 471, 692, 1036, 1114, 1344
ApaLI	GTGCAC	1 cut(s)	1358
ApeKI	GCWGC	2 cut(s)	1713, 1844
ApoI	RAATTY	5 cut(s)	4, 760, 2042, 2076, 2270
AseI	ATTAAT	1 cut(s)	2087
Asp700I	GAANNNNTTC	1 cut(s)	2076
AspLEI	GCGC	2 cut(s)	196, 1654
AspS9I	GGNCC	6 cut(s)	109, 311, 376, 692, 1115, 1193
AsuC2I	CCSGG	4 cut(s)	967, 968, 1040, 1248
AsuHPI	GGTGA	8 cut(s)	424, 563, 848, 954, 1099, 1889, 2071, 2117
AvaI	CYCGRG	2 cut(s)	966, 1265
AvaII	GGWCC	2 cut(s)	376, 1193
AxyI	CCTNAGG	1 cut(s)	720
BaeGI	GKGCMC	1 cut(s)	1362
BaeI	ACNNNNGTAYC	2 cut(s)	781, 814
BalI	TGGCCA	1 cut(s)	473
BamHI	GGATCC	1 cut(s)	674
BbsI	GAAGAC	2 cut(s)	1611, 2140
Bbv12I	GWGCWC	5 cut(s)	446, 932, 993, 1362, 1661
BbvCI	CCTCAGC	1 cut(s)	1521
BbvI	GCAGC	2 cut(s)	1700, 1856
BccI	CCATC	7 cut(s)	275, 908, 1075, 1430, 1574, 1636, 1777
BceAI	ACGGC	2 cut(s)	325, 1488
BcgI	CGANNNNNNTGC	4 cut(s)	763, 797, 1415, 1449
BciT130I	CCWGG	2 cut(s)	448, 1517
BcnI	CCSGG	4 cut(s)	967, 968, 1040, 1248
BcoDI	GTCTC	3 cut(s)	86, 1413, 1540
BfaI	CTAG	3 cut(s)	131, 524, 2238
BisI	GCNGC	3 cut(s)	1714, 1717, 1845
BlsI	GCNGC	3 cut(s)	1715, 1718, 1846
BmcAI	AGTACT	1 cut(s)	1966
Bme1390I	CCNGG	6 cut(s)	448, 967, 968, 1040, 1248, 1517
Bme18I	GGWCC	2 cut(s)	376, 1193
BmeT110I	CYCGRG	2 cut(s)	966, 1265
BmgT120I	GGNCC	6 cut(s)	109, 311, 376, 692, 1115, 1193
BmiI	GGNNCC	2 cut(s)	676, 709
BmrFI	CCNGG	6 cut(s)	448, 967, 968, 1040, 1248, 1517
BmrI	ACTGGG	1 cut(s)	876
BmsI	GCATC	5 cut(s)	655, 771, 1354, 1485, 2020
BmuI	ACTGGG	1 cut(s)	876
BpiI	GAAGAC	2 cut(s)	1611, 2140
BpmI	CTGGAG	1 cut(s)	1989
Bpu10I	CCTNAGC	1 cut(s)	1521
BpuEI	CTTGAG	1 cut(s)	1970
BpuMI	CCSGG	4 cut(s)	967, 968, 1040, 1248
BsaBI	GATNNNNATC	1 cut(s)	285
BsaI	GGTCTC	1 cut(s)	86
BsaJI	CCNNGG	7 cut(s)	96, 513, 965, 966, 1662, 1796, 2097
BsaXI	ACNNNNNCTCC	4 cut(s)	428, 458, 798, 828
Bsc4I	CCNNNNNNNGG	5 cut(s)	690, 1267, 1321, 1443, 1522
Bse118I	RCCGGY	1 cut(s)	941
Bse1I	ACTGG	9 cut(s)	158, 205, 871, 1143, 1204, 1336, 1972, 2064, 2175
Bse21I	CCTNAGG	1 cut(s)	720
Bse3DI	GCAATG	3 cut(s)	189, 903, 1246
Bse8I	GATNNNNATC	1 cut(s)	285
BseBI	CCWGG	2 cut(s)	448, 1517
BseDI	CCNNGG	7 cut(s)	96, 513, 965, 966, 1662, 1796, 2097
BseGI	GGATG	8 cut(s)	1585, 1694, 1788, 1792, 1993, 2008, 2179, 2243
BseJI	GATNNNNATC	1 cut(s)	285
BseLI	CCNNNNNNNGG	5 cut(s)	690, 1267, 1321, 1443, 1522
BseMI	GCAATG	3 cut(s)	189, 903, 1246
BseMII	CTCAG	3 cut(s)	81, 199, 1512
BseNI	ACTGG	9 cut(s)	158, 205, 871, 1143, 1204, 1336, 1972, 2064, 2175
BseRI	GAGGAG	4 cut(s)	242, 611, 1189, 1253
BseSI	GKGCMC	1 cut(s)	1362
BseXI	GCAGC	2 cut(s)	1700, 1856
BseYI	CCCAGC	2 cut(s)	170, 635
BsgI	GTGCAG	1 cut(s)	1181
Bsh1236I	CGCG	4 cut(s)	374, 582, 1654, 1758
Bsh1285I	CGRYCG	1 cut(s)	883
BshFI	GGCC	7 cut(s)	110, 312, 473, 694, 1038, 1116, 1346
BsiEI	CGRYCG	1 cut(s)	883
BsiHKAI	GWGCWC	5 cut(s)	446, 932, 993, 1362, 1661
BsiHKCI	CYCGRG	2 cut(s)	966, 1265
BsiSI	CCGG	4 cut(s)	942, 967, 1039, 1247
BslFI	GGGAC	2 cut(s)	282, 1055
BslI	CCNNNNNNNGG	5 cut(s)	690, 1267, 1321, 1443, 1522
BsmAI	GTCTC	3 cut(s)	86, 1413, 1540
BsmBI	CGTCTC	1 cut(s)	1413
BsmFI	GGGAC	2 cut(s)	282, 1055
BsnI	GGCC	7 cut(s)	110, 312, 473, 694, 1038, 1116, 1346
Bso31I	GGTCTC	1 cut(s)	86
BsoBI	CYCGRG	2 cut(s)	966, 1265
Bsp1286I	GDGCHC	5 cut(s)	446, 932, 993, 1362, 1661
Bsp143I	GATC	3 cut(s)	286, 674, 1678
Bsp19I	CCATGG	1 cut(s)	96
BspACI	CCGC	7 cut(s)	374, 420, 1109, 1654, 1717, 1756, 1775
BspANI	GGCC	7 cut(s)	110, 312, 473, 694, 1038, 1116, 1346
BspCNI	CTCAG	3 cut(s)	82, 198, 1513
BspFNI	CGCG	4 cut(s)	374, 582, 1654, 1758
BspHI	TCATGA	4 cut(s)	993, 1830, 2278, 2285
BspLI	GGNNCC	2 cut(s)	676, 709
BspPI	GGATC	3 cut(s)	669, 682, 1686
BspQI	GCTCTTC	1 cut(s)	1739
BspTNI	GGTCTC	1 cut(s)	86
BsrDI	GCAATG	3 cut(s)	189, 903, 1246
BsrFI	RCCGGY	1 cut(s)	941
BsrI	ACTGG	9 cut(s)	158, 205, 871, 1143, 1204, 1336, 1972, 2064, 2175
BssAI	RCCGGY	1 cut(s)	941
BssECI	CCNNGG	7 cut(s)	96, 513, 965, 966, 1662, 1796, 2097
BssMI	GATC	3 cut(s)	286, 674, 1678
BssT1I	CCWWGG	2 cut(s)	96, 1796
Bst2UI	CCWGG	2 cut(s)	448, 1517
Bst4CI	ACNGT	6 cut(s)	611, 877, 925, 1459, 1813, 1877
Bst6I	CTCTTC	4 cut(s)	805, 1302, 1739, 1968
BstAPI	GCANNNNNTGC	1 cut(s)	1481
BstC8I	GCNNGC	3 cut(s)	943, 1186, 1369
BstDEI	CTNAG	4 cut(s)	90, 185, 720, 1521
BstDSI	CCRYGG	3 cut(s)	96, 1662, 2097
BstEII	GGTNACC	3 cut(s)	895, 1801, 2059
BstENI	CCTNNNNNAGG	2 cut(s)	1265, 1520
BstF5I	GGATG	8 cut(s)	1585, 1694, 1788, 1792, 1993, 2008, 2179, 2243
BstFNI	CGCG	4 cut(s)	374, 582, 1654, 1758
BstHHI	GCGC	2 cut(s)	196, 1654
BstKTI	GATC	3 cut(s)	289, 677, 1681
BstMAI	GTCTC	3 cut(s)	86, 1413, 1540
BstMBI	GATC	3 cut(s)	286, 674, 1678
BstMCI	CGRYCG	1 cut(s)	883
BstMWI	GCNNNNNNNGC	2 cut(s)	419, 1481
BstNI	CCWGG	2 cut(s)	448, 1517
BstNSI	RCATGY	1 cut(s)	742
BstPI	GGTNACC	3 cut(s)	895, 1801, 2059
BstSCI	CCNGG	6 cut(s)	446, 965, 966, 1038, 1246, 1515
BstSLI	GKGCMC	1 cut(s)	1362
BstUI	CGCG	4 cut(s)	374, 582, 1654, 1758
BstV1I	GCAGC	2 cut(s)	1700, 1856
BstV2I	GAAGAC	2 cut(s)	1611, 2140
BstX2I	RGATCY	1 cut(s)	674
BstXI	CCANNNNNNTGG	1 cut(s)	321
BstYI	RGATCY	1 cut(s)	674
Bsu36I	CCTNAGG	1 cut(s)	720
BsuRI	GGCC	7 cut(s)	110, 312, 473, 694, 1038, 1116, 1346
BtgI	CCRYGG	3 cut(s)	96, 1662, 2097
BtsCI	GGATG	8 cut(s)	1585, 1694, 1788, 1792, 1993, 2008, 2179, 2243
BtsI	GCAGTG	2 cut(s)	507, 1482
BtsIMutI	CAGTG	5 cut(s)	198, 507, 1150, 1464, 1482
Cac8I	GCNNGC	3 cut(s)	943, 1186, 1369
CaiI	CAGNNNCTG	1 cut(s)	1481
CciI	TCATGA	4 cut(s)	993, 1830, 2278, 2285
CfoI	GCGC	2 cut(s)	196, 1654
Cfr10I	RCCGGY	1 cut(s)	941
Cfr13I	GGNCC	6 cut(s)	109, 311, 376, 692, 1115, 1193
Cfr9I	CCCGGG	1 cut(s)	966
CseI	GACGC	2 cut(s)	475, 1846
Csp6I	GTAC	9 cut(s)	30, 75, 545, 578, 1165, 1692, 1965, 1996, 2241
CviQI	GTAC	9 cut(s)	30, 75, 545, 578, 1165, 1692, 1965, 1996, 2241
DdeI	CTNAG	4 cut(s)	90, 185, 720, 1521
DpnI	GATC	3 cut(s)	288, 676, 1680
DpnII	GATC	3 cut(s)	286, 674, 1678
DraI	TTTAAA	2 cut(s)	154, 1620
DraIII	CACNNNGTG	1 cut(s)	1321
DrdI	GACNNNNNNGTC	1 cut(s)	1532
DseDI	GACNNNNNNGTC	1 cut(s)	1532
EaeI	YGGCCR	3 cut(s)	471, 1036, 1344
Eam1104I	CTCTTC	4 cut(s)	805, 1302, 1739, 1968
EarI	CTCTTC	4 cut(s)	805, 1302, 1739, 1968
EciI	GGCGGA	1 cut(s)	409
Eco130I	CCWWGG	2 cut(s)	96, 1796
Eco31I	GGTCTC	1 cut(s)	86
Eco47I	GGWCC	2 cut(s)	376, 1193
Eco81I	CCTNAGG	1 cut(s)	720
Eco88I	CYCGRG	2 cut(s)	966, 1265
Eco91I	GGTNACC	3 cut(s)	895, 1801, 2059
EcoNI	CCTNNNNNAGG	2 cut(s)	1265, 1520
EcoO109I	RGGNCCY	2 cut(s)	692, 1193
EcoO65I	GGTNACC	3 cut(s)	895, 1801, 2059
EcoRII	CCWGG	2 cut(s)	446, 1515
EcoT14I	CCWWGG	2 cut(s)	96, 1796
EcoT22I	ATGCAT	1 cut(s)	1500
ErhI	CCWWGG	2 cut(s)	96, 1796
Esp3I	CGTCTC	1 cut(s)	1413
FaqI	GGGAC	2 cut(s)	282, 1055
FauI	CCCGC	1 cut(s)	1768
FauNDI	CATATG	1 cut(s)	1508
Fnu4HI	GCNGC	3 cut(s)	1714, 1717, 1845
FokI	GGATG	8 cut(s)	1592, 1681, 1779, 1795, 2000, 2015, 2186, 2230
Fsp4HI	GCNGC	3 cut(s)	1714, 1717, 1845
FspBI	CTAG	3 cut(s)	131, 524, 2238
GlaI	GCGC	2 cut(s)	195, 1653
GluI	GCNGC	3 cut(s)	1714, 1717, 1845
GsaI	CCCAGC	2 cut(s)	174, 639
GsuI	CTGGAG	1 cut(s)	1989
HaeIII	GGCC	7 cut(s)	110, 312, 473, 694, 1038, 1116, 1346
HapII	CCGG	4 cut(s)	942, 967, 1039, 1247
HgaI	GACGC	2 cut(s)	475, 1846
HhaI	GCGC	2 cut(s)	196, 1654
Hin6I	GCGC	2 cut(s)	194, 1652
HinP1I	GCGC	2 cut(s)	194, 1652
HincII	GTYRAC	2 cut(s)	47, 817
HindII	GTYRAC	2 cut(s)	47, 817
HinfI	GANTC	8 cut(s)	366, 451, 528, 775, 813, 1212, 2183, 2282
HpaII	CCGG	4 cut(s)	942, 967, 1039, 1247
HphI	GGTGA	8 cut(s)	424, 563, 848, 954, 1099, 1889, 2071, 2117
Hpy166II	GTNNAC	9 cut(s)	47, 118, 571, 732, 817, 1228, 1315, 1360, 1387
Hpy188I	TCNGA	5 cut(s)	397, 780, 1073, 1413, 1491
Hpy188III	TCNNGA	8 cut(s)	290, 490, 994, 1394, 1831, 1949, 2279, 2286
Hpy8I	GTNNAC	9 cut(s)	47, 118, 571, 732, 817, 1228, 1315, 1360, 1387
Hpy99I	CGWCG	6 cut(s)	881, 1495, 1862, 1928, 1931, 1940
HpyAV	CCTTC	4 cut(s)	172, 745, 780, 1580
HpyCH4III	ACNGT	6 cut(s)	611, 877, 925, 1459, 1813, 1877
HpyCH4IV	ACGT	6 cut(s)	295, 547, 1067, 1820, 1853, 2165
HpyCH4V	TGCA	8 cut(s)	500, 1162, 1360, 1371, 1462, 1498, 2149, 2260
HpyF10VI	GCNNNNNNNGC	2 cut(s)	419, 1481
HpyF3I	CTNAG	4 cut(s)	90, 185, 720, 1521
HpySE526I	ACGT	6 cut(s)	295, 547, 1067, 1820, 1853, 2165
HspAI	GCGC	2 cut(s)	194, 1652
KroI	GCCGGC	1 cut(s)	941
KroNI	GCCGGC	1 cut(s)	943
Kzo9I	GATC	3 cut(s)	286, 674, 1678
LguI	GCTCTTC	1 cut(s)	1739
LmnI	GCTCC	3 cut(s)	449, 707, 1664
Lsp1109I	GCAGC	2 cut(s)	1700, 1856
LweI	GCATC	5 cut(s)	655, 771, 1354, 1485, 2020
MaeI	CTAG	3 cut(s)	131, 524, 2238
MaeII	ACGT	6 cut(s)	295, 547, 1067, 1820, 1853, 2165
MaeIII	GTNAC	9 cut(s)	836, 895, 960, 1453, 1511, 1801, 1942, 2059, 2166
MalI	GATC	3 cut(s)	288, 676, 1680
MboI	GATC	3 cut(s)	286, 674, 1678
MflI	RGATCY	1 cut(s)	674
MhlI	GDGCHC	5 cut(s)	446, 932, 993, 1362, 1661
MlsI	TGGCCA	1 cut(s)	473
MluCI	AATT	8 cut(s)	4, 760, 1545, 2042, 2053, 2076, 2253, 2270
MluNI	TGGCCA	1 cut(s)	473
MlyI	GAGTC	4 cut(s)	522, 822, 1221, 2177
MmeI	TCCRAC	5 cut(s)	382, 1304, 1436, 1514, 1722
Mox20I	TGGCCA	1 cut(s)	473
Mph1103I	ATGCAT	1 cut(s)	1500
MroNI	GCCGGC	1 cut(s)	941
MroXI	GAANNNNTTC	1 cut(s)	2076
MscI	TGGCCA	1 cut(s)	473
MseI	TTAA	5 cut(s)	153, 744, 1619, 1824, 2087
MslI	CAYNNNNRTG	1 cut(s)	1475
Msp20I	TGGCCA	1 cut(s)	473
MspI	CCGG	4 cut(s)	942, 967, 1039, 1247
MspR9I	CCNGG	6 cut(s)	448, 967, 968, 1040, 1248, 1517
MvaI	CCWGG	2 cut(s)	448, 1517
MvnI	CGCG	4 cut(s)	374, 582, 1654, 1758
MwoI	GCNNNNNNNGC	2 cut(s)	419, 1481
NaeI	GCCGGC	1 cut(s)	943
NciI	CCSGG	4 cut(s)	967, 968, 1040, 1248
NcoI	CCATGG	1 cut(s)	96
NdeI	CATATG	1 cut(s)	1508
NdeII	GATC	3 cut(s)	286, 674, 1678
NgoMIV	GCCGGC	1 cut(s)	941
NlaIV	GGNNCC	2 cut(s)	676, 709
NmuCI	GTSAC	4 cut(s)	836, 960, 1453, 2059
NsiI	ATGCAT	1 cut(s)	1500
NspI	RCATGY	1 cut(s)	742
OliI	CACNNNNGTG	1 cut(s)	1475
PaeR7I	CTCGAG	1 cut(s)	1265
PagI	TCATGA	4 cut(s)	993, 1830, 2278, 2285
PciSI	GCTCTTC	1 cut(s)	1739
PcsI	WCGNNNNNNNCGW	3 cut(s)	1923, 1932, 1935
PdiI	GCCGGC	1 cut(s)	943
PdmI	GAANNNNTTC	1 cut(s)	2076
PfeI	GAWTC	4 cut(s)	366, 451, 775, 2282
PflMI	CCANNNNNTGG	1 cut(s)	1321
PfoI	TCCNGGA	1 cut(s)	446
PkrI	GCNGC	3 cut(s)	1715, 1718, 1846
PleI	GAGTC	4 cut(s)	522, 821, 1220, 2177
PpsI	GAGTC	4 cut(s)	522, 821, 1220, 2177
PpuMI	RGGWCCY	1 cut(s)	1193
PshBI	ATTAAT	1 cut(s)	2087
PsiI	TTATAA	1 cut(s)	1817
Psp1406I	AACGTT	3 cut(s)	1067, 1820, 2165
Psp5II	RGGWCCY	1 cut(s)	1193
Psp6I	CCWGG	2 cut(s)	446, 1515
PspEI	GGTNACC	3 cut(s)	895, 1801, 2059
PspFI	CCCAGC	2 cut(s)	170, 635
PspGI	CCWGG	2 cut(s)	446, 1515
PspN4I	GGNNCC	2 cut(s)	676, 709
PspPI	GGNCC	6 cut(s)	109, 311, 376, 692, 1115, 1193
PspPPI	RGGWCCY	1 cut(s)	1193
PspXI	VCTCGAGB	1 cut(s)	1265
PstNI	CAGNNNCTG	1 cut(s)	1481
PsuI	RGATCY	1 cut(s)	674
RsaI	GTAC	9 cut(s)	31, 76, 546, 579, 1166, 1693, 1966, 1997, 2242
RsaNI	GTAC	9 cut(s)	30, 75, 545, 578, 1165, 1692, 1965, 1996, 2241
RseI	CAYNNNNRTG	1 cut(s)	1475
SapI	GCTCTTC	1 cut(s)	1739
SaqAI	TTAA	5 cut(s)	153, 744, 1619, 1824, 2087
SatI	GCNGC	3 cut(s)	1714, 1717, 1845
Sau3AI	GATC	3 cut(s)	286, 674, 1678
Sau96I	GGNCC	6 cut(s)	109, 311, 376, 692, 1115, 1193
ScaI	AGTACT	1 cut(s)	1966
SchI	GAGTC	4 cut(s)	522, 822, 1221, 2177
ScrFI	CCNGG	6 cut(s)	448, 967, 968, 1040, 1248, 1517
SduI	GDGCHC	5 cut(s)	446, 932, 993, 1362, 1661
SfaNI	GCATC	5 cut(s)	655, 771, 1354, 1485, 2020
Sfr274I	CTCGAG	1 cut(s)	1265
SinI	GGWCC	2 cut(s)	376, 1193
SlaI	CTCGAG	1 cut(s)	1265
SmaI	CCCGGG	1 cut(s)	968
SmiMI	CAYNNNNRTG	1 cut(s)	1475
SmlI	CTYRAG	2 cut(s)	1265, 1949
SmoI	CTYRAG	2 cut(s)	1265, 1949
Sse9I	AATT	8 cut(s)	4, 760, 1545, 2042, 2053, 2076, 2253, 2270
SsiI	CCGC	7 cut(s)	374, 420, 1109, 1654, 1717, 1756, 1775
SspI	AATATT	1 cut(s)	1575
SspMI	CTAG	3 cut(s)	131, 524, 2238
StyD4I	CCNGG	6 cut(s)	446, 965, 966, 1038, 1246, 1515
StyI	CCWWGG	2 cut(s)	96, 1796
TaaI	ACNGT	6 cut(s)	611, 877, 925, 1459, 1813, 1877
TaiI	ACGT	6 cut(s)	298, 550, 1070, 1823, 1856, 2168
TaqI	TCGA	5 cut(s)	773, 1210, 1266, 1425, 1860
TaqII	GACCGA	1 cut(s)	869
TasI	AATT	8 cut(s)	4, 760, 1545, 2042, 2053, 2076, 2253, 2270
TatI	WGTACW	4 cut(s)	1164, 1964, 1995, 2240
TauI	GCSGC	1 cut(s)	1719
TfiI	GAWTC	4 cut(s)	366, 451, 775, 2282
Tru1I	TTAA	5 cut(s)	153, 744, 1619, 1824, 2087
Tru9I	TTAA	5 cut(s)	153, 744, 1619, 1824, 2087
TscAI	CASTG	5 cut(s)	205, 507, 1150, 1464, 1482
TseFI	GTSAC	4 cut(s)	836, 960, 1453, 2059
TseI	GCWGC	2 cut(s)	1713, 1844
Tsp45I	GTSAC	4 cut(s)	836, 960, 1453, 2059
TspDTI	ATGAA	7 cut(s)	17, 1010, 1289, 1617, 1973, 2039, 2274
TspGWI	ACGGA	4 cut(s)	205, 749, 1651, 2114
TspMI	CCCGGG	1 cut(s)	966
TspRI	CASTG	5 cut(s)	205, 507, 1150, 1464, 1482
Van91I	CCANNNNNTGG	1 cut(s)	1321
VneI	GTGCAC	1 cut(s)	1358
VpaK11BI	GGWCC	2 cut(s)	376, 1193
VspI	ATTAAT	1 cut(s)	2087
XagI	CCTNNNNNAGG	2 cut(s)	1265, 1520
XapI	RAATTY	5 cut(s)	4, 760, 2042, 2076, 2270
XceI	RCATGY	1 cut(s)	742
XcmI	CCANNNNNNNNNTGG	2 cut(s)	103, 2167
XhoI	CTCGAG	1 cut(s)	1265
XmaI	CCCGGG	1 cut(s)	966
XmnI	GAANNNNTTC	1 cut(s)	2076
XspI	CTAG	3 cut(s)	131, 524, 2238
ZrmI	AGTACT	1 cut(s)	1966
Zsp2I	ATGCAT	1 cut(s)	1500

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.