Rh5DG216300

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001291
Physical Location & Seq
Reverse (-)
24403674 .. 24410607
6934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG216300.1

Sequence Viewer

Length: 2583 bp
ATGTCGGAGATATCTCCAACGTTGAGAGCAAACAGTACAGCTGATCAAGTTGAAAACTTGGGATTTGCTCTTCCTTATAACAACAGAACCTATACAGTGGTATGTTATAATCTATTTGTTAGGAAATCAAGTAAGCAACTACAACATTTGAGACTTCCCACGGACTTGATTCTCCCTGTCTTCTTAGTCCAAATGGTGAGCATCATGTTCGTTTCTAGCATTCTCGTGCTTGCCCTCAAACGCTTCCACCAACCACGTATTGTCGCTGAAATTCTTGCTGGCTTTCTCTTGGGGCCAACTCTTTTGGGGGAAATTTTGAATAGATTACACATGTTTACCTCTTTTGCTCCATTTGAAAGCGTGCTGAGCTTAGAAACTATAGGAAACCTGGGCCTAGTTTACTACATGTTTCTGGTGGGATTAGAACTTGAACTAAAGCCGGTAATCCGTGCCGGGAAGAAGGCTCTTAGCATTGCCCTCGTAGGCATGGTGGTGGCCATCGCCAGTGGTTGGGGCTTGTTTTTTGTCCTTGACGACAACATCAAGCCTAATCTTAAGTTTCTGAATGCCCCAAATCAGCGAGACAACACACACTCAGGCCACTTGTTCTGGGCACTCGCCCTCGGCACCACAAGCTTCCCAGACGTGGCTCGACTCCTCGCAGATCTTAAGCTCCTCCACTCCGAGCTTGGACGACTTGCATTGTCATCTGCTGTCATCTCCGACCTGTGCTCGTGGTTCCTTTTCATCTTGCTACTAGCACTAACCAATCTGACGGGCCGCTTTTTGGTGGTGGGACTGACTACCCTGGCCTTCGTATGGTTTTGTGTTTTTGTACTCCGCCCGGCTCTTCCAAAGCTGATCCGCTTCGTATCGAAAAACAAAGAGGACAAGAGAGACAACTACAATGAGCTCCATGTGAGCTTTGTTCTCATGGGGGTTCTGGTATGCGCCATGATAACTGATTCGTTAGGTACACTATCCTACGTTGGGGCTTTCGTGTTTGGAGCCATTATGCCGAGGGGAGAGCTCAGCAAATTGGTCGTAGAGAAGATCCAACCCTTTGTGACTGGGATTATGATGCCCCTTTTCTTCTTAATTGTCGGGCTACGAACCAACATGTGGTTTGTATTCAGTTCACATGATTCTTATACGCCCTTTCAATTCAAATATGCAAAGCGTTTCATGCTGGTTCTCTCCCTGGCTTTCTTACCTAAGATTGCCGGCAGTTTTGTTGCCGCTTTAATCCACAAAATGAAACCTTGGAATGCACTGGCTCTGGGAGTAGTCATGAACACCAGAGGCCTAATGGCACTCTTTATTCTCAGCACCGGCAGGGACCTAAAGACCTTGGACCAGAAAACATTCCCAGCAATGATCTTAGTGGTTTGGGCACTGATGATTCCGATCGGACCATTCCTAGCCTACAGTTACAAGACCACCAAGCATTTCGGGCAATACAAATATAGGAACATACGAAGCATAGGATCCGACACAGAGCTTCGCATCCTTGCATGCACCCACGACTCACGCAACTCACCCGCCGTCATCAATCTTCTTGAAGCTTCCAATCCAAGCAGACAGTCCCCGATCCAAGTCTTCGCTGTCCAGCTTGTTGAAATGATTGGCCACACTTCCGCCATGCTCATTGTCCATGATGCTTGCAAACCCAACAATGAAGGGAGCAACCACAAGGCCGGACAAATATCCTCATCCTTGCCCTCCAATCCCTTTGAACTCTACGCAAAGGAAAGAGAGAATGTCCGTGTCCAGGCACTCACCATCGTCTCGGCCTACGCCACAATGCATGAGGACATATGCAACTTTTCGGAGGACAAGTGCATTACCCTCGTGATTGTCCCGTTCCATCAACAATCCACCCTAGACGGAACCATGGAGGAGTCCAACCCTGCTCTCAGGGCAGTCAATAGAAATGTCATTGATAACGCACCATGCTCCGTGGGTGTTCTAGTGGACCGTGGGCTCGGTATATCCCAGATCTTCAAGTCCAACATCAGCAGCCATATTAGCTCCGATGACGTGAGCTACCACCGCTTTGCTGTATTTTTCATTGGAGGGGCAGATGACCGTGAGGCATTAGCGTATGCAAAAAGAATGGCTGGGCATTCCAGGGTTGACCTAACAGTTGTTCGGTTCATCTTAAATACAGAGGCATGCTTAGACGATGCTGACTACGACAACGAAATTATAGAGGCAATGGCAAACACTGGGAGGCAAAAACAGCTTGATGACTTGTACCTGGATGGTTTTAGGGTCCATACAATGCACAATTCCTCCATAAAGTTCGTAGAGCAGGTGGTGAATAGCTGGGAAGAAACTCTGGACGCCATAAACGACATGGAGGGTGATTATGATTTGTATATAATGGGAAGACGACATGGAAGCAGCAGCACGTTGGCAGTGACATCAGCATTGGACAGCAGCGACTGTGATGAGCTAGGTACTCTGGCAGAGGCGTTAGTGTCTTCTAGCTTTTTTAAGGTTTACACATCTATTCTCATAGTGCAGCAAGGTGCTGCTGTAGAGGAAATACAAGATGAGTCGGATAACCAGGAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

860

Amino Acids

95.64

Weight (kDa)

6.28

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 70 - 468 2.2e-52 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 533 - 664 1.4e-17 Plant cation/H(+) antiporter domain
CHX17_C PF23259 687 - 844 2e-12 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 78, 108
AarI CACCTGC 1 cut(s) 2305
Acc36I ACCTGC 1 cut(s) 2305
AccB1I GGYRCC 1 cut(s) 626
AccB7I CCANNNNNTGG 2 cut(s) 510, 1122
AciI CCGC 7 cut(s) 781, 841, 865, 1239, 1542, 1638, 2053
AclI AACGTT 1 cut(s) 20
AclWI GGATC 5 cut(s) 856, 1048, 1482, 1495, 1585
AcoI YGGCCR 2 cut(s) 495, 1627
AcsI RAATTY 2 cut(s) 270, 312
AcyI GRCGYC 1 cut(s) 2346
AfaI GTAC 5 cut(s) 37, 837, 976, 2258, 2464
AfiI CCNNNNNNNGG 7 cut(s) 510, 646, 787, 819, 990, 1122, 1771
AflII CTTAAG 2 cut(s) 554, 668
AflIII ACRYGT 3 cut(s) 330, 405, 1119
AjiI CACGTC 2 cut(s) 646, 2041
AjnI CCWGG 7 cut(s) 387, 807, 1200, 1770, 2129, 2259, 2571
Alw21I GWGCWC 3 cut(s) 734, 915, 1032
Alw26I GTCTC 4 cut(s) 145, 576, 891, 1792
AlwI GGATC 5 cut(s) 856, 1048, 1482, 1495, 1585
AlwNI CAGNNNCTG 1 cut(s) 2448
ApeKI GCWGC 6 cut(s) 2019, 2406, 2409, 2442, 2527, 2537
ApoI RAATTY 2 cut(s) 270, 312
Asp700I GAANNNNTTC 1 cut(s) 1364
AspLEI GCGC 1 cut(s) 953
AspS9I GGNCC 8 cut(s) 293, 391, 778, 1339, 1354, 1412, 1975, 2275
AsuC2I CCSGG 2 cut(s) 454, 845
AsuHPI GGTGA 5 cut(s) 208, 1530, 1771, 2332, 2378
AvaII GGWCC 5 cut(s) 1339, 1354, 1412, 1975, 2275
BaeGI GKGCMC 2 cut(s) 616, 1396
BalI TGGCCA 2 cut(s) 497, 1629
BamHI GGATCC 1 cut(s) 1487
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 3 cut(s) 915, 1032, 1986
BauI CACGAG 3 cut(s) 224, 733, 1850
BbsI GAAGAC 4 cut(s) 172, 1591, 2398, 2478
Bbv12I GWGCWC 3 cut(s) 734, 915, 1032
BbvI GCAGC 6 cut(s) 2031, 2418, 2421, 2454, 2524, 2539
BccI CCATC 4 cut(s) 506, 1790, 1875, 2258
BceAI ACGGC 1 cut(s) 1529
BcgI CGANNNNNNTGC 8 cut(s) 190, 224, 460, 494, 1024, 1058, 1831, 1865
BciT130I CCWGG 7 cut(s) 389, 809, 1202, 1772, 2131, 2261, 2573
BclI TGATCA 1 cut(s) 43
BcnI CCSGG 2 cut(s) 454, 845
BcoDI GTCTC 4 cut(s) 145, 576, 891, 1792
BfaI CTAG 8 cut(s) 216, 395, 758, 1421, 1883, 1970, 2459, 2490
BfmI CTRYAG 3 cut(s) 378, 1426, 2541
BfrI CTTAAG 2 cut(s) 554, 668
BfuAI ACCTGC 1 cut(s) 2305
BglII AGATCT 2 cut(s) 664, 1998
BisI GCNGC 8 cut(s) 781, 1239, 2020, 2407, 2410, 2443, 2528, 2538
BlpI GCTNAGC 2 cut(s) 365, 1031
BlsI GCNGC 8 cut(s) 782, 1240, 2021, 2408, 2411, 2444, 2529, 2539
Bme1390I CCNGG 9 cut(s) 389, 454, 809, 845, 1202, 1772, 2131, 2261, 2573
Bme18I GGWCC 5 cut(s) 1339, 1354, 1412, 1975, 2275
BmgBI CACGTC 2 cut(s) 646, 2041
BmgT120I GGNCC 8 cut(s) 293, 391, 778, 1339, 1354, 1412, 1975, 2275
BmiI GGNNCC 8 cut(s) 294, 628, 740, 1009, 1340, 1489, 1891, 2276
BmrFI CCNGG 9 cut(s) 389, 454, 809, 845, 1202, 1772, 2131, 2261, 2573
BmrI ACTGGG 2 cut(s) 1080, 2238
BmsI GCATC 5 cut(s) 210, 1071, 1515, 1648, 2176
BmuI ACTGGG 2 cut(s) 1080, 2238
BpiI GAAGAC 4 cut(s) 172, 1591, 2398, 2478
Bpu1102I GCTNAGC 2 cut(s) 365, 1031
BpuMI CCSGG 2 cut(s) 454, 845
BsaAI YACGTR 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 1406
BsaHI GRCGYC 1 cut(s) 2346
BsaXI ACNNNNNCTCC 4 cut(s) 1823, 1853, 2279, 2309
Bsc4I CCNNNNNNNGG 7 cut(s) 510, 646, 787, 819, 990, 1122, 1771
Bse118I RCCGGY 3 cut(s) 439, 1223, 1331
Bse1I ACTGG 4 cut(s) 504, 1075, 1278, 2233
Bse3DI GCAATG 3 cut(s) 471, 1380, 2223
Bse8I GATNNNNATC 1 cut(s) 1406
BseBI CCWGG 7 cut(s) 389, 809, 1202, 1772, 2131, 2261, 2573
BseGI GGATG 3 cut(s) 1506, 1712, 2269
BseJI GATNNNNATC 1 cut(s) 1406
BseLI CCNNNNNNNGG 7 cut(s) 510, 646, 787, 819, 990, 1122, 1771
BseMI GCAATG 3 cut(s) 471, 1380, 2223
BseMII CTCAG 5 cut(s) 356, 609, 1045, 1339, 1930
BseNI ACTGG 4 cut(s) 504, 1075, 1278, 2233
BseRI GAGGAG 3 cut(s) 647, 665, 1913
BseSI GKGCMC 2 cut(s) 616, 1396
BseXI GCAGC 6 cut(s) 2031, 2418, 2421, 2454, 2524, 2539
BseYI CCCAGC 3 cut(s) 1369, 2120, 2328
BsgI GTGCAG 1 cut(s) 2546
Bsh1285I CGRYCG 1 cut(s) 1410
BshNI GGYRCC 1 cut(s) 626
BsiEI CGRYCG 1 cut(s) 1410
BsiHKAI GWGCWC 3 cut(s) 734, 915, 1032
BsiSI CCGG 6 cut(s) 440, 453, 845, 1224, 1332, 1698
BslFI GGGAC 4 cut(s) 810, 1352, 1570, 1844
BslI CCNNNNNNNGG 7 cut(s) 510, 646, 787, 819, 990, 1122, 1771
BsmAI GTCTC 4 cut(s) 145, 576, 891, 1792
BsmBI CGTCTC 1 cut(s) 1792
BsmFI GGGAC 4 cut(s) 810, 1352, 1570, 1844
BsmI GAATGC 4 cut(s) 219, 571, 1273, 2125
Bsp1286I GDGCHC 6 cut(s) 616, 734, 915, 1032, 1396, 1986
Bsp143I GATC 9 cut(s) 43, 664, 861, 1053, 1377, 1407, 1487, 1590, 1998
Bsp1720I GCTNAGC 2 cut(s) 365, 1031
Bsp19I CCATGG 1 cut(s) 1893
BspACI CCGC 7 cut(s) 781, 841, 865, 1239, 1542, 1638, 2053
BspCNI CTCAG 5 cut(s) 357, 608, 1044, 1338, 1929
BspHI TCATGA 1 cut(s) 1290
BspLI GGNNCC 8 cut(s) 294, 628, 740, 1009, 1340, 1489, 1891, 2276
BspMI ACCTGC 1 cut(s) 2305
BspPI GGATC 5 cut(s) 856, 1048, 1482, 1495, 1585
BspQI GCTCTTC 2 cut(s) 75, 855
BspT107I GGYRCC 1 cut(s) 626
BspTI CTTAAG 2 cut(s) 554, 668
BsrDI GCAATG 3 cut(s) 471, 1380, 2223
BsrFI RCCGGY 3 cut(s) 439, 1223, 1331
BsrI ACTGG 4 cut(s) 504, 1075, 1278, 2233
BssAI RCCGGY 3 cut(s) 439, 1223, 1331
BssMI GATC 9 cut(s) 43, 664, 861, 1053, 1377, 1407, 1487, 1590, 1998
BssNI GRCGYC 1 cut(s) 2346
BssSI CACGAG 3 cut(s) 224, 733, 1850
BssT1I CCWWGG 3 cut(s) 1262, 1350, 1893
Bst2BI CACGAG 3 cut(s) 224, 733, 1850
Bst2UI CCWGG 7 cut(s) 389, 809, 1202, 1772, 2131, 2261, 2573
Bst4CI ACNGT 8 cut(s) 35, 97, 1430, 1584, 1979, 2090, 2146, 2450
Bst6I CTCTTC 2 cut(s) 75, 855
BstACI GRCGYC 1 cut(s) 2346
BstAFI CTTAAG 2 cut(s) 554, 668
BstBAI YACGTR 1 cut(s) 257
BstC8I GCNNGC 7 cut(s) 231, 280, 362, 1225, 1516, 1663, 2176
BstDSI CCRYGG 4 cut(s) 159, 1893, 1959, 1978
BstF5I GGATG 3 cut(s) 1506, 1712, 2269
BstHHI GCGC 1 cut(s) 953
BstKTI GATC 9 cut(s) 46, 667, 864, 1056, 1380, 1410, 1490, 1593, 2001
BstMAI GTCTC 4 cut(s) 145, 576, 891, 1792
BstMBI GATC 9 cut(s) 43, 664, 861, 1053, 1377, 1407, 1487, 1590, 1998
BstMCI CGRYCG 1 cut(s) 1410
BstMWI GCNNNNNNNGC 8 cut(s) 366, 633, 1186, 1453, 1919, 2028, 2052, 2242
BstNI CCWGG 7 cut(s) 389, 809, 1202, 1772, 2131, 2261, 2573
BstNSI RCATGY 5 cut(s) 334, 409, 1123, 1518, 2178
BstSCI CCNGG 9 cut(s) 387, 452, 807, 843, 1200, 1770, 2129, 2259, 2571
BstSFI CTRYAG 3 cut(s) 378, 1426, 2541
BstSLI GKGCMC 2 cut(s) 616, 1396
BstV1I GCAGC 6 cut(s) 2031, 2418, 2421, 2454, 2524, 2539
BstV2I GAAGAC 4 cut(s) 172, 1591, 2398, 2478
BstX2I RGATCY 4 cut(s) 664, 1053, 1487, 1998
BstYI RGATCY 4 cut(s) 664, 1053, 1487, 1998
BtgI CCRYGG 4 cut(s) 159, 1893, 1959, 1978
BtgZI GCGATG 1 cut(s) 484
BtrI CACGTC 2 cut(s) 646, 2041
BtsCI GGATG 3 cut(s) 1506, 1712, 2269
BtsI GCAGTG 1 cut(s) 2427
BtsIMutI CAGTG 6 cut(s) 102, 511, 1271, 1394, 2226, 2427
BveI ACCTGC 1 cut(s) 2305
Cac8I GCNNGC 7 cut(s) 231, 280, 362, 1225, 1516, 1663, 2176
CaiI CAGNNNCTG 1 cut(s) 2448
CciI TCATGA 1 cut(s) 1290
CfoI GCGC 1 cut(s) 953
Cfr10I RCCGGY 3 cut(s) 439, 1223, 1331
Cfr13I GGNCC 8 cut(s) 293, 391, 778, 1339, 1354, 1412, 1975, 2275
CseI GACGC 1 cut(s) 2354
Csp6I GTAC 5 cut(s) 36, 836, 975, 2257, 2463
CviQI GTAC 5 cut(s) 36, 836, 975, 2257, 2463
DpnI GATC 9 cut(s) 45, 666, 863, 1055, 1379, 1409, 1489, 1592, 2000
DpnII GATC 9 cut(s) 43, 664, 861, 1053, 1377, 1407, 1487, 1590, 1998
EaeI YGGCCR 2 cut(s) 495, 1627
Eam1104I CTCTTC 2 cut(s) 75, 855
EarI CTCTTC 2 cut(s) 75, 855
EciI GGCGGA 2 cut(s) 830, 1627
Ecl136II GAGCTC 2 cut(s) 913, 1030
Eco130I CCWWGG 3 cut(s) 1262, 1350, 1893
Eco147I AGGCCT 1 cut(s) 1305
Eco24I GRGCYC 3 cut(s) 915, 1032, 1986
Eco32I GATATC 1 cut(s) 12
Eco47I GGWCC 5 cut(s) 1339, 1354, 1412, 1975, 2275
Eco53kI GAGCTC 2 cut(s) 913, 1030
EcoICRI GAGCTC 2 cut(s) 913, 1030
EcoO109I RGGNCCY 1 cut(s) 1339
EcoRII CCWGG 7 cut(s) 387, 807, 1200, 1770, 2129, 2259, 2571
EcoRV GATATC 1 cut(s) 12
EcoT14I CCWWGG 3 cut(s) 1262, 1350, 1893
EcoT22I ATGCAT 1 cut(s) 1809
EcoT38I GRGCYC 3 cut(s) 915, 1032, 1986
ErhI CCWWGG 3 cut(s) 1262, 1350, 1893
Esp3I CGTCTC 1 cut(s) 1792
FaqI GGGAC 4 cut(s) 810, 1352, 1570, 1844
FauI CCCGC 1 cut(s) 1549
FauNDI CATATG 1 cut(s) 1817
FbaI TGATCA 1 cut(s) 43
Fnu4HI GCNGC 8 cut(s) 781, 1239, 2020, 2407, 2410, 2443, 2528, 2538
FokI GGATG 3 cut(s) 1493, 1699, 2276
FriOI GRGCYC 3 cut(s) 915, 1032, 1986
Fsp4HI GCNGC 8 cut(s) 781, 1239, 2020, 2407, 2410, 2443, 2528, 2538
FspBI CTAG 8 cut(s) 216, 395, 758, 1421, 1883, 1970, 2459, 2490
GlaI GCGC 1 cut(s) 952
GluI GCNGC 8 cut(s) 781, 1239, 2020, 2407, 2410, 2443, 2528, 2538
GsaI CCCAGC 3 cut(s) 1373, 2124, 2332
HapII CCGG 6 cut(s) 440, 453, 845, 1224, 1332, 1698
HgaI GACGC 1 cut(s) 2354
HhaI GCGC 1 cut(s) 953
Hin1I GRCGYC 1 cut(s) 2346
Hin6I GCGC 1 cut(s) 951
HinP1I GCGC 1 cut(s) 951
HincII GTYRAC 1 cut(s) 2137
HindII GTYRAC 1 cut(s) 2137
HindIII AAGCTT 2 cut(s) 634, 1563
HinfI GANTC 8 cut(s) 169, 654, 965, 1145, 1402, 1526, 1901, 2561
HpaII CCGG 6 cut(s) 440, 453, 845, 1224, 1332, 1698
HphI GGTGA 5 cut(s) 208, 1530, 1771, 2332, 2378
Hpy166II GTNNAC 7 cut(s) 336, 400, 977, 1139, 1975, 2137, 2506
Hpy188III TCNNGA 4 cut(s) 1291, 1559, 1852, 2342
Hpy8I GTNNAC 7 cut(s) 336, 400, 977, 1139, 1975, 2137, 2506
HpyAV CCTTC 3 cut(s) 454, 823, 1673
HpyCH4III ACNGT 8 cut(s) 35, 97, 1430, 1584, 1979, 2090, 2146, 2450
HpyCH4IV ACGT 6 cut(s) 20, 256, 645, 987, 2040, 2414
HpyF10VI GCNNNNNNNGC 8 cut(s) 366, 633, 1186, 1453, 1919, 2028, 2052, 2242
HpySE526I ACGT 6 cut(s) 20, 256, 645, 987, 2040, 2414
Hsp92I GRCGYC 1 cut(s) 2346
HspAI GCGC 1 cut(s) 951
KroI GCCGGC 1 cut(s) 1223
KroNI GCCGGC 1 cut(s) 1225
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 9 cut(s) 43, 664, 861, 1053, 1377, 1407, 1487, 1590, 1998
LguI GCTCTTC 2 cut(s) 75, 855
LmnI GCTCC 7 cut(s) 352, 678, 918, 1007, 1683, 1961, 2036
Lsp1109I GCAGC 6 cut(s) 2031, 2418, 2421, 2454, 2524, 2539
LweI GCATC 5 cut(s) 210, 1071, 1515, 1648, 2176
MaeI CTAG 8 cut(s) 216, 395, 758, 1421, 1883, 1970, 2459, 2490
MaeII ACGT 6 cut(s) 20, 256, 645, 987, 2040, 2414
MaeIII GTNAC 3 cut(s) 1066, 1430, 2422
MalI GATC 9 cut(s) 45, 666, 863, 1055, 1379, 1409, 1489, 1592, 2000
MboI GATC 9 cut(s) 43, 664, 861, 1053, 1377, 1407, 1487, 1590, 1998
MflI RGATCY 4 cut(s) 664, 1053, 1487, 1998
MhlI GDGCHC 6 cut(s) 616, 734, 915, 1032, 1396, 1986
MlsI TGGCCA 2 cut(s) 497, 1629
MluCI AATT 7 cut(s) 270, 312, 1037, 1098, 1163, 2205, 2290
MluNI TGGCCA 2 cut(s) 497, 1629
MlyI GAGTC 4 cut(s) 648, 1520, 1910, 2570
MmeI TCCRAC 7 cut(s) 41, 747, 1081, 1515, 1929, 2034, 2544
Mox20I TGGCCA 2 cut(s) 497, 1629
Mph1103I ATGCAT 1 cut(s) 1809
MroNI GCCGGC 1 cut(s) 1223
MroXI GAANNNNTTC 1 cut(s) 1364
MscI TGGCCA 2 cut(s) 497, 1629
MseI TTAA 6 cut(s) 555, 669, 1097, 1244, 2162, 2499
MslI CAYNNNNRTG 2 cut(s) 224, 491
Msp20I TGGCCA 2 cut(s) 497, 1629
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 2 cut(s) 554, 668
MspI CCGG 6 cut(s) 440, 453, 845, 1224, 1332, 1698
MspR9I CCNGG 9 cut(s) 389, 454, 809, 845, 1202, 1772, 2131, 2261, 2573
Mva1269I GAATGC 4 cut(s) 219, 571, 1273, 2125
MvaI CCWGG 7 cut(s) 389, 809, 1202, 1772, 2131, 2261, 2573
MwoI GCNNNNNNNGC 8 cut(s) 366, 633, 1186, 1453, 1919, 2028, 2052, 2242
NaeI GCCGGC 1 cut(s) 1225
NciI CCSGG 2 cut(s) 454, 845
NcoI CCATGG 1 cut(s) 1893
NdeI CATATG 1 cut(s) 1817
NdeII GATC 9 cut(s) 43, 664, 861, 1053, 1377, 1407, 1487, 1590, 1998
NgoMIV GCCGGC 1 cut(s) 1223
NlaIV GGNNCC 8 cut(s) 294, 628, 740, 1009, 1340, 1489, 1891, 2276
NmeAIII GCCGAG 3 cut(s) 603, 1044, 1769
NmuCI GTSAC 2 cut(s) 1066, 2422
NsiI ATGCAT 1 cut(s) 1809
NspI RCATGY 5 cut(s) 334, 409, 1123, 1518, 2178
PaeI GCATGC 2 cut(s) 1518, 2178
PagI TCATGA 1 cut(s) 1290
PaqCI CACCTGC 1 cut(s) 2305
PceI AGGCCT 1 cut(s) 1305
PciI ACATGT 3 cut(s) 330, 405, 1119
PciSI GCTCTTC 2 cut(s) 75, 855
PcsI WCGNNNNNNNCGW 1 cut(s) 2352
PctI GAATGC 4 cut(s) 219, 571, 1273, 2125
PdiI GCCGGC 1 cut(s) 1225
PdmI GAANNNNTTC 1 cut(s) 1364
PfeI GAWTC 4 cut(s) 169, 965, 1145, 1402
PflMI CCANNNNNTGG 2 cut(s) 510, 1122
PkrI GCNGC 8 cut(s) 782, 1240, 2021, 2408, 2411, 2444, 2529, 2539
Ple19I CGATCG 1 cut(s) 1410
PleI GAGTC 4 cut(s) 648, 1520, 1909, 2569
PpsI GAGTC 4 cut(s) 648, 1520, 1909, 2569
Ppu21I YACGTR 1 cut(s) 257
PpuMI RGGWCCY 1 cut(s) 1339
PscI ACATGT 3 cut(s) 330, 405, 1119
PsiI TTATAA 2 cut(s) 78, 108
Psp124BI GAGCTC 2 cut(s) 915, 1032
Psp1406I AACGTT 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 1339
Psp6I CCWGG 7 cut(s) 387, 807, 1200, 1770, 2129, 2259, 2571
PspFI CCCAGC 3 cut(s) 1369, 2120, 2328
PspGI CCWGG 7 cut(s) 387, 807, 1200, 1770, 2129, 2259, 2571
PspN4I GGNNCC 8 cut(s) 294, 628, 740, 1009, 1340, 1489, 1891, 2276
PspPI GGNCC 8 cut(s) 293, 391, 778, 1339, 1354, 1412, 1975, 2275
PspPPI RGGWCCY 1 cut(s) 1339
PstNI CAGNNNCTG 1 cut(s) 2448
PsuI RGATCY 4 cut(s) 664, 1053, 1487, 1998
PvuI CGATCG 1 cut(s) 1410
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 5 cut(s) 37, 837, 976, 2258, 2464
RsaNI GTAC 5 cut(s) 36, 836, 975, 2257, 2463
RseI CAYNNNNRTG 2 cut(s) 224, 491
SacI GAGCTC 2 cut(s) 915, 1032
SapI GCTCTTC 2 cut(s) 75, 855
SaqAI TTAA 6 cut(s) 555, 669, 1097, 1244, 2162, 2499
SatI GCNGC 8 cut(s) 781, 1239, 2020, 2407, 2410, 2443, 2528, 2538
Sau3AI GATC 9 cut(s) 43, 664, 861, 1053, 1377, 1407, 1487, 1590, 1998
Sau96I GGNCC 8 cut(s) 293, 391, 778, 1339, 1354, 1412, 1975, 2275
SchI GAGTC 4 cut(s) 648, 1520, 1910, 2570
ScrFI CCNGG 9 cut(s) 389, 454, 809, 845, 1202, 1772, 2131, 2261, 2573
SduI GDGCHC 6 cut(s) 616, 734, 915, 1032, 1396, 1986
SfaNI GCATC 5 cut(s) 210, 1071, 1515, 1648, 2176
SfcI CTRYAG 3 cut(s) 378, 1426, 2541
SinI GGWCC 5 cut(s) 1339, 1354, 1412, 1975, 2275
SmiMI CAYNNNNRTG 2 cut(s) 224, 491
SmlI CTYRAG 2 cut(s) 554, 668
SmoI CTYRAG 2 cut(s) 554, 668
SphI GCATGC 2 cut(s) 1518, 2178
Sse9I AATT 7 cut(s) 270, 312, 1037, 1098, 1163, 2205, 2290
SseBI AGGCCT 1 cut(s) 1305
SsiI CCGC 7 cut(s) 781, 841, 865, 1239, 1542, 1638, 2053
SspMI CTAG 8 cut(s) 216, 395, 758, 1421, 1883, 1970, 2459, 2490
SstI GAGCTC 2 cut(s) 915, 1032
StuI AGGCCT 1 cut(s) 1305
StyD4I CCNGG 9 cut(s) 387, 452, 807, 843, 1200, 1770, 2129, 2259, 2571
StyI CCWWGG 3 cut(s) 1262, 1350, 1893
TaaI ACNGT 8 cut(s) 35, 97, 1430, 1584, 1979, 2090, 2146, 2450
TaiI ACGT 6 cut(s) 23, 259, 648, 990, 2043, 2417
TaqI TCGA 2 cut(s) 652, 875
TasI AATT 7 cut(s) 270, 312, 1037, 1098, 1163, 2205, 2290
TatI WGTACW 2 cut(s) 35, 835
TauI GCSGC 2 cut(s) 783, 1241
TfiI GAWTC 4 cut(s) 169, 965, 1145, 1402
Tru1I TTAA 6 cut(s) 555, 669, 1097, 1244, 2162, 2499
Tru9I TTAA 6 cut(s) 555, 669, 1097, 1244, 2162, 2499
TscAI CASTG 6 cut(s) 102, 511, 1278, 1401, 2233, 2427
TseFI GTSAC 2 cut(s) 1066, 2422
TseI GCWGC 6 cut(s) 2019, 2406, 2409, 2442, 2527, 2537
Tsp45I GTSAC 2 cut(s) 1066, 2422
TspDTI ATGAA 7 cut(s) 736, 1174, 1271, 1307, 1692, 2059, 2146
TspGWI ACGGA 5 cut(s) 176, 437, 1754, 1902, 1948
TspRI CASTG 6 cut(s) 102, 511, 1278, 1401, 2233, 2427
Van91I CCANNNNNTGG 2 cut(s) 510, 1122
Vha464I CTTAAG 2 cut(s) 554, 668
VpaK11BI GGWCC 5 cut(s) 1339, 1354, 1412, 1975, 2275
XapI RAATTY 2 cut(s) 270, 312
XceI RCATGY 5 cut(s) 334, 409, 1123, 1518, 2178
XcmI CCANNNNNNNNNTGG 3 cut(s) 686, 1306, 2356
XmnI GAANNNNTTC 1 cut(s) 1364
XspI CTAG 8 cut(s) 216, 395, 758, 1421, 1883, 1970, 2459, 2490
Zsp2I ATGCAT 1 cut(s) 1809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.