Rroxscaffold_3G00232440

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001291
Physical Location & Seq
Reverse (-)
18074992 .. 18078689
3698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00232440.1

Sequence Viewer

Length: 2577 bp
ATGACGTCAATGAATTCTTCGATGCTAGAATTGATAAAGAAGGGATATAGTTTGCAGATAGACACAAACGATAATGTGACTGTATGTTATTTGAAAAATAGAATCCGTACAGATGGGTTGTGGCAAGCTGAAAACCCTCTGCTAGATTCTCTTCATGTACTTGTCATTCAGCTGATTCTCATCTTGTTCGTTAATCACGTTCTCGTGTTCCCCCTGAGACCCTTGCGCCAACCACGTATTGTAGCTGATATTCTTGCTGGTGTTCTACTGGGTCCAACTGCTATCGGGGGTCACCTTATTATAAGAAAATTCGCACTTTCTTTTAACAGCTTGTTGACCTTAGAGACTGTAGCAAACTTGGGCTTGGTTTACTACATGTTCCTACTTGGTATAGAGCTTGATTTCAGACCAGTAATACGCACCGGGAAGAAGGCTCTTAGCATTGCTGTTGCTGGCATTGTTGTTTCTGTACCCGTCGGCTGGTCCTTGCATTACTATATACTTCGGAATCAAAAAAGTAAAGTTTCGAAGGAGGATGAGCAGGAACGTTTATGGATTCAAGGACCCTTGTTTTGGGGCATTACACTAGCCACCACCAATTTCGCCGATCTCGCTCGAATTCTTGCAGACCTCAAGCTCCTCCACTCTGATATTGGACGGCTTGCCTTGTCTGCAGCCGTTATCTCCGACTTGTTCTCAGGGTTTCTTTTTATGCTGGCAATGGCTTTTGTTAGTATTAATTGGTGGTCGCTGGCGTTGACCTCGGCTTTCATAGCAATCTTTGTATTTCTAGTTCGCCCAGCTCTCTCCTGGATACTCCAGTGCACAACAAAAAAGGACAGCTATAGTGAGCTCCATGTCTGCTTCATTCTAACCTGGATTGTGATATGTGGATTCATAACCGATGCATGTGGTGCGCATTCTATAGTGGGAGCTTTTGTATTGGGAGCTATTATGCCTAGGGAACTAGAGATCAAAAGCATTCTTGTGGAGAGGATTGGCAACTTTGTGTCCAGCATAATGATGCCTCTCTTCTACTTGGTGGTTGGGCTAAGGTTCAATGTTGATCGGATATTATATACAGAGGAAGGAAAACCAAATGGAACTACCGAGTTCGATGTCATGGTGGCTGTCGCTCTGGCTTTTACAGCTAAAATTGTTGGCACTTCTATCGCAGCCTTAATGAACAAAATTACACTTCGAGATAGTATGGTTCTTGGAGTTCTCATGAACACCAAGGGCTTATTGGCACTCATTATACTCAGCTCTGCTCGAGATCTAAAGGTCTTGGATCAGCAAATGTTTGCGATAATGCTAGTAGTGGTTTGGGGGATCACAGTTCCCGTTGGACCCTTCCTAGCCTTCGCTTACAAGTCCAGCAAGCGTTCCCCACTATACAAACTCAAAAACATACAGAGTGTTGGAACCAACAAAGATTTTCGGATCCTTGCATGCACTCACACTTTAAACAACACATCTGGCATGATTGAGCTTATTGAGGCTTCTAATCCATTAGAACAATCCCCAATCCATGTATTTGCCGTCCAACTAGTGGAACTTACCGGCCATGCCTCGGCCATGCTCATTGTGCATGACGCTTGCAAGTCCAGCAAACATCGCCACGAGGAGGAACAATCTAACTCTGCCTCCAATCCGTTCAAAACCTATGCGTTGCAGAGAGAAAATGTCTCTGTACAAGCACTCACAGTAGTGTCTGCCTACAACACCATGCATGAGGACATATGCACCCTAGCTGAGGATAAGAATGTTAACCTTGTAATCATTCCGTTCCACAAGAAATCTAACATTGATGATGGGACGTTAGAGGATTCCAATCCTTCTTTCTTGGGCATCAATAAGAATGTGATCGAAAACTCGCCGTGCTCTGTATGTGTCTTTGTGGATCGTGGGCTTGCTGCATGTAGAATATTTAGTAACTATAACAATGATTCTTCTGGCTCTGCTAGAGGAGTTAGAGGACATAAGTTTGCCATGTTGTTCCTTGGAGGGACAGATGATCGAGAGGCATTAGCATATGCATGGAGGATGGCTGGCCATTCTGGGATTCACTTGACGGTTATTCGGCTTACCTTGCCTAAAACGTCGACAGTAGGCCCGATGAGACTAGATGACAAAGACAATTTTTTGGAGGAAATGGCAAACACTCAAAGGCAGAACCAATTTGATAACTTATGCATTGATGAGTTCAGGCTTAAGACTATGAACAATTCCGCTATACGACTATTTGAAGAGCAAGTAAGTAGTTGGGAAGAAATTCACAATATATTAAGGAGCATGGAAGGACAGTATGCTCTGTGCATAGTGGGAAAAGGCCATGGAATGTCATTATCCTTCAAAGAAACATCAATGGATTACGATGACGACGATTATGATGAGCTTGGAGCACTAGGAGAAGCATTGGTATCTTCGAGATTTATGACGAATACATCAATTGTGATAATAAAGCAGGGTGCTCTTTTGGATGAAAGAAAGGTAGAGAATAAAATGGGGCATGTAGAAGAACAGTTAGGTCATACAATTTGGCAACCTCCCCCAAAAAAACTACTGACAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

858

Amino Acids

95.42

Weight (kDa)

6.53

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 67 - 448 1.3e-55 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 512 - 641 3.8e-16 Plant cation/H(+) antiporter domain
CHX17_C PF23259 664 - 823 3.9e-12 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 302
AatII GACGTC 1 cut(s) 8
Acc16I TGCGCA 1 cut(s) 918
AccB7I CCANNNNNTGG 1 cut(s) 1552
AccI GTMKAC 1 cut(s) 2105
AciI CCGC 1 cut(s) 2232
AclI AACGTT 1 cut(s) 547
AclWI GGATC 5 cut(s) 1299, 1340, 1438, 1451, 1911
AcoI YGGCCR 3 cut(s) 1564, 1575, 2053
AcsI RAATTY 4 cut(s) 13, 308, 618, 2274
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 109, 159, 471, 1695
AfiI CCNNNNNNNGG 6 cut(s) 480, 573, 1552, 1573, 1627, 1756
AflII CTTAAG 1 cut(s) 2213
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 6 cut(s) 94, 560, 1060, 1660, 2249, 2356
AhlI ACTAGT 1 cut(s) 1549
AjnI CCWGG 2 cut(s) 809, 875
AjuI GAANNNNNNNTTGG 2 cut(s) 1826, 1858
AleI CACNNNNGTG 1 cut(s) 1709
Alw21I GWGCWC 5 cut(s) 827, 855, 1886, 2407, 2476
Alw26I GTCTC 4 cut(s) 211, 338, 1693, 2116
Alw44I GTGCAC 1 cut(s) 823
AlwI GGATC 5 cut(s) 1299, 1340, 1438, 1451, 1911
Ama87I CYCGRG 1 cut(s) 1272
AoxI GGCC 5 cut(s) 1564, 1575, 2053, 2113, 2332
ApaLI GTGCAC 1 cut(s) 823
ApeKI GCWGC 3 cut(s) 674, 1175, 1916
ApoI RAATTY 4 cut(s) 13, 308, 618, 2274
ArsI GACNNNNNNTTYG 2 cut(s) 555, 587
AseI ATTAAT 1 cut(s) 738
Asp700I GAANNNNTTC 1 cut(s) 2274
AspA2I CCTAGG 1 cut(s) 959
AspLEI GCGC 2 cut(s) 228, 919
AspS9I GGNCC 5 cut(s) 272, 483, 563, 1349, 2114
AsuC2I CCSGG 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 284
AsuII TTCGAA 1 cut(s) 527
AvaI CYCGRG 1 cut(s) 1272
AvaII GGWCC 4 cut(s) 272, 483, 563, 1349
AvrII CCTAGG 1 cut(s) 959
BaeGI GKGCMC 1 cut(s) 827
BaeI ACNNNNGTAYC 2 cut(s) 806, 839
BalI TGGCCA 1 cut(s) 2055
BamHI GGATCC 1 cut(s) 1443
BanII GRGCYC 1 cut(s) 855
BauI CACGAG 2 cut(s) 203, 1622
Bbv12I GWGCWC 5 cut(s) 827, 855, 1886, 2407, 2476
BbvCI CCTCAGC 1 cut(s) 1755
BbvI GCAGC 3 cut(s) 686, 1187, 1903
BccI CCATC 3 cut(s) 107, 1808, 2041
BceAI ACGGC 4 cut(s) 662, 674, 1526, 1864
BcgI CGANNNNNNTGC 2 cut(s) 1153, 1187
BciT130I CCWGG 2 cut(s) 811, 877
BciVI GTATCC 1 cut(s) 807
BcnI CCSGG 1 cut(s) 424
BcoDI GTCTC 4 cut(s) 211, 338, 1693, 2116
BcuI ACTAGT 1 cut(s) 1549
BfmI CTRYAG 4 cut(s) 348, 672, 844, 924
BfrI CTTAAG 1 cut(s) 2213
BfuI GTATCC 1 cut(s) 807
BglII AGATCT 1 cut(s) 1276
BisI GCNGC 3 cut(s) 675, 1176, 1917
BlnI CCTAGG 1 cut(s) 959
BlsI GCNGC 3 cut(s) 676, 1177, 1918
Bme1390I CCNGG 3 cut(s) 424, 811, 877
Bme18I GGWCC 4 cut(s) 272, 483, 563, 1349
BmeT110I CYCGRG 1 cut(s) 1272
BmgT120I GGNCC 5 cut(s) 272, 483, 563, 1349, 2114
BmiI GGNNCC 5 cut(s) 273, 565, 1351, 1426, 1445
BmrFI CCNGG 3 cut(s) 424, 811, 877
BmrI ACTGGG 1 cut(s) 278
BmsI GCATC 4 cut(s) 12, 895, 1014, 1860
BmuI ACTGGG 1 cut(s) 278
BpmI CTGGAG 1 cut(s) 803
Bpu10I CCTNAGC 2 cut(s) 1052, 1755
Bpu14I TTCGAA 1 cut(s) 527
BpuEI CTTGAG 1 cut(s) 617
BpuMI CCSGG 1 cut(s) 424
BsaAI YACGTR 1 cut(s) 236
BsaBI GATNNNNATC 2 cut(s) 179, 1833
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 211
BsaJI CCNNGG 6 cut(s) 762, 959, 1236, 1572, 2002, 2335
BsaXI ACNNNNNCTCC 4 cut(s) 924, 954, 1631, 1661
Bsc4I CCNNNNNNNGG 6 cut(s) 480, 573, 1552, 1573, 1627, 1756
Bse118I RCCGGY 1 cut(s) 1562
Bse1I ACTGG 3 cut(s) 273, 410, 820
Bse3DI GCAATG 2 cut(s) 441, 726
Bse8I GATNNNNATC 2 cut(s) 179, 1833
BseBI CCWGG 2 cut(s) 811, 877
BseDI CCNNGG 6 cut(s) 762, 959, 1236, 1572, 2002, 2335
BseGI GGATG 3 cut(s) 541, 2052, 2488
BseJI GATNNNNATC 2 cut(s) 179, 1833
BseLI CCNNNNNNNGG 6 cut(s) 480, 573, 1552, 1573, 1627, 1756
BseMI GCAATG 2 cut(s) 441, 726
BseMII CTCAG 4 cut(s) 206, 711, 1276, 1746
BseNI ACTGG 3 cut(s) 273, 410, 820
BseRI GAGGAG 3 cut(s) 629, 1640, 1983
BseSI GKGCMC 1 cut(s) 827
BseXI GCAGC 3 cut(s) 686, 1187, 1903
BseYI CCCAGC 1 cut(s) 799
BshFI GGCC 5 cut(s) 1566, 1577, 2055, 2115, 2334
BsiHKAI GWGCWC 5 cut(s) 827, 855, 1886, 2407, 2476
BsiHKCI CYCGRG 1 cut(s) 1272
BsiSI CCGG 2 cut(s) 423, 1563
BslFI GGGAC 2 cut(s) 1831, 2023
BslI CCNNNNNNNGG 6 cut(s) 480, 573, 1552, 1573, 1627, 1756
BsmAI GTCTC 4 cut(s) 211, 338, 1693, 2116
BsmFI GGGAC 2 cut(s) 1831, 2023
BsmI GAATGC 2 cut(s) 919, 981
BsnI GGCC 5 cut(s) 1566, 1577, 2055, 2115, 2334
Bso31I GGTCTC 1 cut(s) 211
BsoBI CYCGRG 1 cut(s) 1272
Bsp119I TTCGAA 1 cut(s) 527
Bsp1286I GDGCHC 5 cut(s) 827, 855, 1886, 2407, 2476
Bsp1407I TGTACA 1 cut(s) 1693
Bsp19I CCATGG 1 cut(s) 2335
BspACI CCGC 1 cut(s) 2232
BspANI GGCC 5 cut(s) 1566, 1577, 2055, 2115, 2334
BspCNI CTCAG 4 cut(s) 207, 710, 1275, 1747
BspHI TCATGA 1 cut(s) 1227
BspLI GGNNCC 5 cut(s) 273, 565, 1351, 1426, 1445
BspMAI CTGCAG 1 cut(s) 676
BspPI GGATC 5 cut(s) 1299, 1340, 1438, 1451, 1911
BspQI GCTCTTC 1 cut(s) 2244
BspT104I TTCGAA 1 cut(s) 527
BspTI CTTAAG 1 cut(s) 2213
BspTNI GGTCTC 1 cut(s) 211
BsrDI GCAATG 2 cut(s) 441, 726
BsrFI RCCGGY 1 cut(s) 1562
BsrGI TGTACA 1 cut(s) 1693
BsrI ACTGG 3 cut(s) 273, 410, 820
BssAI RCCGGY 1 cut(s) 1562
BssECI CCNNGG 6 cut(s) 762, 959, 1236, 1572, 2002, 2335
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 203, 1622
BssT1I CCWWGG 4 cut(s) 959, 1236, 2002, 2335
Bst2BI CACGAG 2 cut(s) 203, 1622
Bst2UI CCWGG 2 cut(s) 811, 877
Bst4CI ACNGT 9 cut(s) 82, 349, 1339, 1708, 2077, 2110, 2307, 2526, 2572
Bst6I CTCTTC 3 cut(s) 156, 1037, 2244
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 2213
BstAPI GCANNNNNTGC 1 cut(s) 914
BstAUI TGTACA 1 cut(s) 1693
BstBAI YACGTR 1 cut(s) 236
BstBI TTCGAA 1 cut(s) 527
BstDEI CTNAG 7 cut(s) 215, 340, 437, 697, 1052, 1262, 1755
BstDSI CCRYGG 1 cut(s) 2335
BstEII GGTNACC 1 cut(s) 290
BstENI CCTNNNNNAGG 1 cut(s) 1754
BstF5I GGATG 3 cut(s) 541, 2052, 2488
BstHHI GCGC 2 cut(s) 228, 919
BstMAI GTCTC 4 cut(s) 211, 338, 1693, 2116
BstMWI GCNNNNNNNGC 9 cut(s) 611, 671, 773, 914, 1148, 1588, 1608, 1617, 2092
BstNI CCWGG 2 cut(s) 811, 877
BstNSI RCATGY 5 cut(s) 379, 912, 1455, 1923, 2516
BstPI GGTNACC 1 cut(s) 290
BstSCI CCNGG 3 cut(s) 422, 809, 875
BstSFI CTRYAG 4 cut(s) 348, 672, 844, 924
BstSLI GKGCMC 1 cut(s) 827
BstV1I GCAGC 3 cut(s) 686, 1187, 1903
BstX2I RGATCY 2 cut(s) 1276, 1443
BstYI RGATCY 2 cut(s) 1276, 1443
BsuI GTATCC 1 cut(s) 807
BsuRI GGCC 5 cut(s) 1566, 1577, 2055, 2115, 2334
BtgI CCRYGG 1 cut(s) 2335
BtgZI GCGATG 1 cut(s) 1601
BtsCI GGATG 3 cut(s) 541, 2052, 2488
BtsIMutI CAGTG 2 cut(s) 827, 2577
CciI TCATGA 1 cut(s) 1227
CfoI GCGC 2 cut(s) 228, 919
Cfr10I RCCGGY 1 cut(s) 1562
Cfr13I GGNCC 5 cut(s) 272, 483, 563, 1349, 2114
CseI GACGC 1 cut(s) 1604
Csp6I GTAC 4 cut(s) 108, 158, 470, 1694
CviQI GTAC 4 cut(s) 108, 158, 470, 1694
DdeI CTNAG 7 cut(s) 215, 340, 437, 697, 1052, 1262, 1755
DraI TTTAAA 1 cut(s) 1467
EaeI YGGCCR 3 cut(s) 1564, 1575, 2053
Eam1104I CTCTTC 3 cut(s) 156, 1037, 2244
EarI CTCTTC 3 cut(s) 156, 1037, 2244
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 4 cut(s) 959, 1236, 2002, 2335
Eco24I GRGCYC 1 cut(s) 855
Eco31I GGTCTC 1 cut(s) 211
Eco47I GGWCC 4 cut(s) 272, 483, 563, 1349
Eco53kI GAGCTC 1 cut(s) 853
Eco88I CYCGRG 1 cut(s) 1272
Eco91I GGTNACC 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 853
EcoNI CCTNNNNNAGG 1 cut(s) 1754
EcoO109I RGGNCCY 1 cut(s) 563
EcoO65I GGTNACC 1 cut(s) 290
EcoRI GAATTC 2 cut(s) 13, 618
EcoRII CCWGG 2 cut(s) 809, 875
EcoT14I CCWWGG 4 cut(s) 959, 1236, 2002, 2335
EcoT22I ATGCAT 4 cut(s) 910, 1734, 2041, 2198
EcoT38I GRGCYC 1 cut(s) 855
ErhI CCWWGG 4 cut(s) 959, 1236, 2002, 2335
FaqI GGGAC 2 cut(s) 1831, 2023
FauNDI CATATG 2 cut(s) 1742, 2035
FblI GTMKAC 1 cut(s) 2105
Fnu4HI GCNGC 3 cut(s) 675, 1176, 1917
FokI GGATG 3 cut(s) 548, 2059, 2495
FriOI GRGCYC 1 cut(s) 855
Fsp4HI GCNGC 3 cut(s) 675, 1176, 1917
FspAI RTGCGCAY 1 cut(s) 918
FspI TGCGCA 1 cut(s) 918
GlaI GCGC 2 cut(s) 227, 918
GluI GCNGC 3 cut(s) 675, 1176, 1917
GsaI CCCAGC 1 cut(s) 803
GsuI CTGGAG 1 cut(s) 803
HaeIII GGCC 5 cut(s) 1566, 1577, 2055, 2115, 2334
HapII CCGG 2 cut(s) 423, 1563
HgaI GACGC 1 cut(s) 1604
HhaI GCGC 2 cut(s) 228, 919
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 226, 917
HinP1I GCGC 2 cut(s) 226, 917
HincII GTYRAC 4 cut(s) 336, 759, 1771, 2106
HindII GTYRAC 4 cut(s) 336, 759, 1771, 2106
HinfI GANTC 9 cut(s) 102, 146, 175, 508, 556, 894, 1829, 1949, 2065
HpaI GTTAAC 1 cut(s) 1771
HpaII CCGG 2 cut(s) 423, 1563
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 6 cut(s) 336, 370, 759, 825, 1771, 2106
Hpy188I TCNGA 6 cut(s) 407, 507, 649, 688, 1071, 1443
Hpy188III TCNNGA 5 cut(s) 1202, 1228, 1274, 2021, 2430
Hpy8I GTNNAC 6 cut(s) 336, 370, 759, 825, 1771, 2106
Hpy99I CGWCG 3 cut(s) 479, 2107, 2387
HpyAV CCTTC 9 cut(s) 34, 424, 523, 1082, 1363, 1372, 1848, 2294, 2362
HpyCH4III ACNGT 9 cut(s) 82, 349, 1339, 1708, 2077, 2110, 2307, 2526, 2572
HpyCH4IV ACGT 6 cut(s) 5, 198, 235, 547, 1820, 2102
HpyF10VI GCNNNNNNNGC 9 cut(s) 611, 671, 773, 914, 1148, 1588, 1608, 1617, 2092
HpyF3I CTNAG 7 cut(s) 215, 340, 437, 697, 1052, 1262, 1755
HpySE526I ACGT 6 cut(s) 5, 198, 235, 547, 1820, 2102
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 226, 917
KspAI GTTAAC 1 cut(s) 1771
LguI GCTCTTC 1 cut(s) 2244
LmnI GCTCC 6 cut(s) 642, 858, 932, 947, 2292, 2402
Lsp1109I GCAGC 3 cut(s) 686, 1187, 1903
LweI GCATC 4 cut(s) 12, 895, 1014, 1860
MaeII ACGT 6 cut(s) 5, 198, 235, 547, 1820, 2102
MaeIII GTNAC 3 cut(s) 76, 290, 1934
MboII GAAGA 9 cut(s) 9, 143, 439, 1024, 1944, 2261, 2282, 2418, 2531
MfeI CAATTG 1 cut(s) 2451
MflI RGATCY 2 cut(s) 1276, 1443
MhlI GDGCHC 5 cut(s) 827, 855, 1886, 2407, 2476
MlsI TGGCCA 1 cut(s) 2055
MluNI TGGCCA 1 cut(s) 2055
MmeI TCCRAC 5 cut(s) 299, 711, 1327, 1402, 1570
Mox20I TGGCCA 1 cut(s) 2055
Mph1103I ATGCAT 4 cut(s) 910, 1734, 2041, 2198
MroXI GAANNNNTTC 1 cut(s) 2274
MscI TGGCCA 1 cut(s) 2055
MseI TTAA 8 cut(s) 192, 324, 738, 1181, 1466, 1770, 2214, 2288
MslI CAYNNNNRTG 4 cut(s) 986, 1022, 1709, 2038
Msp20I TGGCCA 1 cut(s) 2055
MspA1I CMGCKG 1 cut(s) 172
MspCI CTTAAG 1 cut(s) 2213
MspI CCGG 2 cut(s) 423, 1563
MspR9I CCNGG 3 cut(s) 424, 811, 877
MunI CAATTG 1 cut(s) 2451
Mva1269I GAATGC 2 cut(s) 919, 981
MvaI CCWGG 2 cut(s) 811, 877
MwoI GCNNNNNNNGC 9 cut(s) 611, 671, 773, 914, 1148, 1588, 1608, 1617, 2092
NciI CCSGG 1 cut(s) 424
NcoI CCATGG 1 cut(s) 2335
NdeI CATATG 2 cut(s) 1742, 2035
NlaIV GGNNCC 5 cut(s) 273, 565, 1351, 1426, 1445
NmeAIII GCCGAG 2 cut(s) 743, 1553
NmuCI GTSAC 2 cut(s) 76, 290
NsbI TGCGCA 1 cut(s) 918
NsiI ATGCAT 4 cut(s) 910, 1734, 2041, 2198
NspI RCATGY 5 cut(s) 379, 912, 1455, 1923, 2516
NspV TTCGAA 1 cut(s) 527
OliI CACNNNNGTG 1 cut(s) 1709
PaeI GCATGC 1 cut(s) 1455
PaeR7I CTCGAG 1 cut(s) 1272
PagI TCATGA 1 cut(s) 1227
PciI ACATGT 1 cut(s) 375
PciSI GCTCTTC 1 cut(s) 2244
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 2382
PctI GAATGC 2 cut(s) 919, 981
PdmI GAANNNNTTC 1 cut(s) 2274
PfeI GAWTC 9 cut(s) 102, 146, 175, 508, 556, 894, 1829, 1949, 2065
PflMI CCANNNNNTGG 1 cut(s) 1552
PfoI TCCNGGA 1 cut(s) 809
PkrI GCNGC 3 cut(s) 676, 1177, 1918
Ppu21I YACGTR 1 cut(s) 236
PpuMI RGGWCCY 1 cut(s) 563
PscI ACATGT 1 cut(s) 375
PshBI ATTAAT 1 cut(s) 738
PsiI TTATAA 1 cut(s) 302
Psp124BI GAGCTC 1 cut(s) 855
Psp1406I AACGTT 1 cut(s) 547
Psp5II RGGWCCY 1 cut(s) 563
Psp6I CCWGG 2 cut(s) 809, 875
PspEI GGTNACC 1 cut(s) 290
PspFI CCCAGC 1 cut(s) 799
PspGI CCWGG 2 cut(s) 809, 875
PspN4I GGNNCC 5 cut(s) 273, 565, 1351, 1426, 1445
PspPI GGNCC 5 cut(s) 272, 483, 563, 1349, 2114
PspPPI RGGWCCY 1 cut(s) 563
PsrI GAACNNNNNNTAC 2 cut(s) 777, 809
PstI CTGCAG 1 cut(s) 676
PsuI RGATCY 2 cut(s) 1276, 1443
PvuII CAGCTG 1 cut(s) 172
RsaI GTAC 4 cut(s) 109, 159, 471, 1695
RsaNI GTAC 4 cut(s) 108, 158, 470, 1694
RseI CAYNNNNRTG 4 cut(s) 986, 1022, 1709, 2038
SacI GAGCTC 1 cut(s) 855
SalI GTCGAC 1 cut(s) 2104
SapI GCTCTTC 1 cut(s) 2244
SaqAI TTAA 8 cut(s) 192, 324, 738, 1181, 1466, 1770, 2214, 2288
SatI GCNGC 3 cut(s) 675, 1176, 1917
Sau96I GGNCC 5 cut(s) 272, 483, 563, 1349, 2114
ScrFI CCNGG 3 cut(s) 424, 811, 877
SduI GDGCHC 5 cut(s) 827, 855, 1886, 2407, 2476
SfaNI GCATC 4 cut(s) 12, 895, 1014, 1860
SfcI CTRYAG 4 cut(s) 348, 672, 844, 924
Sfr274I CTCGAG 1 cut(s) 1272
SfuI TTCGAA 1 cut(s) 527
SinI GGWCC 4 cut(s) 272, 483, 563, 1349
SlaI CTCGAG 1 cut(s) 1272
SmiMI CAYNNNNRTG 4 cut(s) 986, 1022, 1709, 2038
SmlI CTYRAG 3 cut(s) 632, 1272, 2213
SmoI CTYRAG 3 cut(s) 632, 1272, 2213
SpeI ACTAGT 1 cut(s) 1549
SphI GCATGC 1 cut(s) 1455
SsiI CCGC 1 cut(s) 2232
SspI AATATT 1 cut(s) 1929
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 3 cut(s) 422, 809, 875
StyI CCWWGG 4 cut(s) 959, 1236, 2002, 2335
TaaI ACNGT 9 cut(s) 82, 349, 1339, 1708, 2077, 2110, 2307, 2526, 2572
TaiI ACGT 6 cut(s) 8, 201, 238, 550, 1823, 2105
TatI WGTACW 2 cut(s) 157, 1693
TfiI GAWTC 9 cut(s) 102, 146, 175, 508, 556, 894, 1829, 1949, 2065
Tru1I TTAA 8 cut(s) 192, 324, 738, 1181, 1466, 1770, 2214, 2288
Tru9I TTAA 8 cut(s) 192, 324, 738, 1181, 1466, 1770, 2214, 2288
TscAI CASTG 2 cut(s) 827, 2577
TseFI GTSAC 2 cut(s) 76, 290
TseI GCWGC 3 cut(s) 674, 1175, 1916
Tsp45I GTSAC 2 cut(s) 76, 290
TspDTI ATGAA 9 cut(s) 26, 143, 760, 856, 886, 1199, 1244, 2237, 2499
TspGWI ACGGA 3 cut(s) 95, 1644, 1776
TspRI CASTG 2 cut(s) 827, 2577
Van91I CCANNNNNTGG 1 cut(s) 1552
Vha464I CTTAAG 1 cut(s) 2213
VneI GTGCAC 1 cut(s) 823
VpaK11BI GGWCC 4 cut(s) 272, 483, 563, 1349
VspI ATTAAT 1 cut(s) 738
XagI CCTNNNNNAGG 1 cut(s) 1754
XapI RAATTY 4 cut(s) 13, 308, 618, 2274
XceI RCATGY 5 cut(s) 379, 912, 1455, 1923, 2516
XcmI CCANNNNNNNNNTGG 3 cut(s) 650, 807, 1243
XhoI CTCGAG 1 cut(s) 1272
XmaJI CCTAGG 1 cut(s) 959
XmiI GTMKAC 1 cut(s) 2105
XmnI GAANNNNTTC 1 cut(s) 2274
ZraI GACGTC 1 cut(s) 6
Zsp2I ATGCAT 4 cut(s) 910, 1734, 2041, 2198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.