MD02G1296900.v1.1

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001291
Physical Location & Seq
Reverse (-)
35231362 .. 35235188
3827 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1296900.v1.1.491

Sequence Viewer

Length: 2496 bp
ATGAATGAACAACCAAGCGTGCATGCGCTACCACCAGCGATTGCAAAAGGTACTTCATTTCAGGAAGGTACTTGGGAGATATGTTATAACATGACAGTGTACAAGACAGGAGAGATATGGCGTGTGCAGAGTACATTTTTCAGATCTGTTCCGATGATCATTGTCCAACTGATCGCCGCCATGTCCATCACTCGCCTTCTTTATCATCTCTTCAAGCCCTTGCACCAACCTCGTATTGTCTCCGATATTCTTGGTGGCGTGATGTTGGGTCCGGCATTCGTTGGTACTATCATATTTGCTCTTCAATACGTAATCCCTTTCTTCAGCCTGGTCAACATAGAAACCGTGGCAAACTTAGGGCTTGGTTATCACATGTTCATCATGGGTTTAGAGCTTGAGTTTGTACCTATAATCCGGGCTGGAAAAAGGGCTCTTAGCGTTGCCGTAGTTGGCAGTCTCGTTTCCTTGATCTGGGGTTGGCTTTTATATCGCTTCGTACTCTTCAATGATTATGGTAGGGCACTGGCTGAGCAAATTGCCAACGACAACGGCTACGCCTTCTGGGGCATTGCCCTGGCCTCCACCAACTTCACCGACCTTGCCCAAGTCCTCGCCGACAAAAAGCTCCTCCACTCTGATTTCGGCCGCCTCGCCTTGTCCTCGGCGGTTATCTCCGACGTGTTATCCTGGCTTCTTCTCCTTGCGGGCATGACTTTCGCATATACTGACCAATCGATCGGGGCCATGAGCGCCGTGATATTCATACTACTATGCGTTTTTGTACTACGTCCGGCTATCCCGTGGTTCATCCGCAATACAACCACCGACAACGATAGCAGCGGATACAACGAGCAGCAAGTCCGCTTCATTCTGTCGGTGGTTGTGCTTTTCGGGTACGTAAGTGACAATTTTGGGTCGCACTCCATTTTGGGACCCTTTATGCTGGGAACCATTATTCCTAGTGGAGAGCTGAAGAAGGTCCTGGTTGACAGGATCCAAACCTTCGTGAGTCACCTTCTGATGCCTCTATACTTCTTAACCGTCGGGCTAAGAACTAATTTTAGCTATATTTTAACCAACGAAAATCCAAAGTTCACACTTGCAGATACTAACCTGTGGCGTGTCGTGGCGGTTGTCGTTCTTGCTAACATTCCGAAAATTTTGAGTAATTATATCATCGGCTTGATGTACAAAATGTCACGTGGAGATAGTTTTGCTCTTGGGATACTCATGAACACCAAAGGCTTAATGGGACTCATAATACTCGGCACTGGCAAGGACATAAAGGTCTTGGACAACCGAACGTTTGGAGTAATGATGATTGCGATGTGGTTAATGACGCTTCCGGTCGGACCCTTTCTAGCCCTCGTGTTCAAGCCCACCAAGAACACCGCAGAATACAAACTCAGGAACATACAAAGCGTAGGACCTGAGACGGAGCTAAAAATCCTTACTTGCACCCATACCTCAAGTAGCGTCTCCGGCATGATCGACCTCCTCGAGGCTTCAAATCCGACGAGACAATCCCCAATCTGCATCTTCGCCGTTCAACTTGTGGAGATCACAGACCATGGTTCGGCCATGCTCATCGTGCAGGAAGTTTGCAAAGAAAACTCTAACCGAAGCAACCAGATGCAACCCGAGTACATCATGTCCGAAAATCGATTCGAAAAATACGCGAAGAATAGAGGAAACGTTTCTATCCAAACACTCACAGCCTTTTCTTCCTACAACACCATGCATGAATACATATGCAATCTGGCCAACGAGAAGTTCATCAACATTATTATAACCCCATTCCACAATCAATTTTCCATTGACGGAACCATGGAGGATACCAACCCTAATTTGAAGGGGGTCAACAACAACTTGATAAAAAATTCGCCGTGCACGGTGGGTATTTTCGTTGATCGTGGGCTTAGTGTGTCCCAAATTAGCGAGTCCAGAAATGGCAGCGGTTCCTCCCCAGGGTACCATCTATTCGCCATGTTTTTCTTTGGAGGACCAGATGACCGCGAGGCATTAACGTATGCATGGAGGATGGCCGGGCATCCTCGGGTTAATCTAAGGGTTGTCCGGTTTATTGTGGTCCCAAGTAATGCAACATCAGGCAAATTCACATGCGATGACCAGAACGACAACGACGGGGACATCGATCAAGCGGTAAATAGTCGGATGCAGAAGCATCTCGACGACACGTGCATGGATGAGTTTAGGCTTAAGTCGATGCACAATCGCAGCACACAATTCGTGGAGGAGATTGTGAAGAGCTGGGAAGAAACTGTAAATATTGTAAGGGGCCTGGAGAGTGAATATGATCTGTACTTAGTGGGAAGAAGATACGAAAGCTCATCCCCAGCGTGGTCGTTATTGGATTATTGCAGCGATTACGACGAACTGGGACCTCTTGGGGACTTATTAGTTTCTTCAACCTTTAATCCATCCGTTCTTATTGTGCAGCAGGGTACCCTTGTGGATAAACACCGGTGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

832

Amino Acids

92.85

Weight (kDa)

6.01

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 62 - 454 7.9e-43 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 512 - 642 4.4e-14 Plant cation/H(+) antiporter domain
CHX17_C PF23259 663 - 823 5.9e-15 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 87, 1790
AasI GACNNNNNNGTC 1 cut(s) 1286
AbsI CCTCGAGG 1 cut(s) 1499
Acc65I GGTACC 2 cut(s) 1971, 2465
AccB1I GGYRCC 2 cut(s) 1971, 2465
AccII CGCG 2 cut(s) 1679, 2016
AclI AACGTT 2 cut(s) 1304, 1695
AclWI GGATC 2 cut(s) 988, 1001
AcoI YGGCCR 4 cut(s) 643, 1578, 1761, 2043
AcsI RAATTY 3 cut(s) 1158, 1879, 2114
AcuI CTGAAG 2 cut(s) 307, 992
AcvI CACGTG 2 cut(s) 1202, 2199
AfiI CCNNNNNNNGG 5 cut(s) 471, 1501, 1576, 1968, 2361
AflII CTTAAG 1 cut(s) 2219
AflIII ACRYGT 3 cut(s) 372, 678, 2196
AgeI ACCGGT 1 cut(s) 2484
AgsI TTSAA 8 cut(s) 214, 305, 505, 1375, 1509, 1550, 1852, 2430
AjiI CACGTC 1 cut(s) 679
AjnI CCWGG 6 cut(s) 327, 573, 686, 981, 1966, 2301
AjuI GAANNNNNNNTTGG 2 cut(s) 1757, 1789
AleI CACNNNNGTG 1 cut(s) 2487
AloI GAACNNNNNNTCC 2 cut(s) 940, 972
AluBI AGCT 7 cut(s) 394, 625, 970, 1065, 1441, 2271, 2349
AluI AGCT 7 cut(s) 394, 625, 970, 1065, 1441, 2271, 2349
Alw21I GWGCWC 1 cut(s) 1892
Alw26I GTCTC 5 cut(s) 244, 461, 1427, 1483, 1513
Alw44I GTGCAC 1 cut(s) 1888
AlwI GGATC 2 cut(s) 988, 1001
Ama87I CYCGRG 3 cut(s) 1499, 1640, 2055
AoxI GGCC 7 cut(s) 576, 643, 741, 1578, 1761, 2043, 2299
ApaLI GTGCAC 1 cut(s) 1888
ApeKI GCWGC 6 cut(s) 837, 853, 1953, 2238, 2382, 2458
ApoI RAATTY 3 cut(s) 1158, 1879, 2114
AsiGI ACCGGT 1 cut(s) 2484
Asp700I GAANNNNTTC 1 cut(s) 1696
Asp718I GGTACC 2 cut(s) 1971, 2465
AspLEI GCGC 2 cut(s) 28, 752
AsuC2I CCSGG 2 cut(s) 416, 2047
AsuHPI GGTGA 2 cut(s) 583, 1004
AsuII TTCGAA 1 cut(s) 1668
AvaI CYCGRG 3 cut(s) 1499, 1640, 2055
AvaII GGWCC 8 cut(s) 269, 932, 979, 1352, 1427, 2003, 2089, 2402
BaeGI GKGCMC 2 cut(s) 523, 1892
BaeI ACNNNNGTAYC 4 cut(s) 886, 919, 2457, 2490
BalI TGGCCA 1 cut(s) 1763
BamHI GGATCC 1 cut(s) 993
BanI GGYRCC 2 cut(s) 1971, 2465
BanII GRGCYC 1 cut(s) 433
BauI CACGAG 1 cut(s) 1367
BbrPI CACGTG 2 cut(s) 1202, 2199
Bbv12I GWGCWC 1 cut(s) 1892
BbvI GCAGC 6 cut(s) 849, 865, 1965, 2250, 2394, 2470
BccI CCATC 4 cut(s) 194, 1983, 2035, 2449
BceAI ACGGC 5 cut(s) 428, 565, 737, 1529, 1870
BcgI CGANNNNNNTGC 6 cut(s) 212, 246, 697, 731, 2230, 2264
BciT130I CCWGG 6 cut(s) 329, 575, 688, 983, 1968, 2303
BciVI GTATCC 3 cut(s) 836, 1218, 1828
BclI TGATCA 1 cut(s) 156
BcnI CCSGG 2 cut(s) 416, 2047
BcoDI GTCTC 5 cut(s) 244, 461, 1427, 1483, 1513
BfaI CTAG 2 cut(s) 960, 1361
BfoI RGCGCY 1 cut(s) 753
BfrI CTTAAG 1 cut(s) 2219
BfuI GTATCC 3 cut(s) 836, 1218, 1828
BglII AGATCT 1 cut(s) 143
BisI GCNGC 8 cut(s) 177, 646, 838, 854, 1954, 2239, 2383, 2459
BlpI GCTNAGC 1 cut(s) 528
BlsI GCNGC 8 cut(s) 178, 647, 839, 855, 1955, 2240, 2384, 2460
Bme1390I CCNGG 8 cut(s) 329, 416, 575, 688, 983, 1968, 2047, 2303
Bme18I GGWCC 8 cut(s) 269, 932, 979, 1352, 1427, 2003, 2089, 2402
BmeT110I CYCGRG 3 cut(s) 1499, 1640, 2055
BmgBI CACGTC 1 cut(s) 679
BmrFI CCNGG 8 cut(s) 329, 416, 575, 688, 983, 1968, 2047, 2303
BmrI ACTGGG 1 cut(s) 2408
BmsI GCATC 7 cut(s) 1011, 1545, 1623, 2059, 2166, 2194, 2217
BmuI ACTGGG 1 cut(s) 2408
BpmI CTGGAG 1 cut(s) 2324
Bpu1102I GCTNAGC 1 cut(s) 528
Bpu14I TTCGAA 1 cut(s) 1668
BpuEI CTTGAG 2 cut(s) 416, 1453
BpuMI CCSGG 2 cut(s) 416, 2047
Bsa29I ATCGAT 3 cut(s) 734, 1663, 2154
BsaAI YACGTR 4 cut(s) 310, 898, 1202, 2199
BsaJI CCNNGG 9 cut(s) 345, 573, 660, 800, 1570, 1827, 1966, 1967, 2054
BsaWI WCCGGW 3 cut(s) 1345, 2076, 2484
Bsc4I CCNNNNNNNGG 5 cut(s) 471, 1501, 1576, 1968, 2361
Bse118I RCCGGY 1 cut(s) 2484
Bse1I ACTGG 3 cut(s) 528, 1276, 2403
Bse3DI GCAATG 1 cut(s) 567
BseBI CCWGG 6 cut(s) 329, 575, 688, 983, 1968, 2303
BseCI ATCGAT 3 cut(s) 734, 1663, 2154
BseDI CCNNGG 9 cut(s) 345, 573, 660, 800, 1570, 1827, 1966, 1967, 2054
BseGI GGATG 7 cut(s) 807, 2046, 2050, 2181, 2212, 2351, 2441
BseLI CCNNNNNNNGG 5 cut(s) 471, 1501, 1576, 1968, 2361
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 3 cut(s) 519, 1420, 1422
BseNI ACTGG 3 cut(s) 528, 1276, 2403
BseRI GAGGAG 3 cut(s) 617, 1487, 2270
BseSI GKGCMC 2 cut(s) 523, 1892
BseX3I CGGCCG 1 cut(s) 643
BseXI GCAGC 6 cut(s) 849, 865, 1965, 2250, 2394, 2470
BseYI CCCAGC 3 cut(s) 943, 2271, 2356
BsgI GTGCAG 3 cut(s) 146, 1613, 2477
Bsh1236I CGCG 2 cut(s) 1679, 2016
Bsh1285I CGRYCG 3 cut(s) 646, 738, 1350
BshFI GGCC 7 cut(s) 578, 645, 743, 1580, 1763, 2045, 2301
BshNI GGYRCC 2 cut(s) 1971, 2465
BshTI ACCGGT 1 cut(s) 2484
BshVI ATCGAT 3 cut(s) 734, 1663, 2154
BsiEI CGRYCG 3 cut(s) 646, 738, 1350
BsiHKAI GWGCWC 1 cut(s) 1892
BsiHKCI CYCGRG 3 cut(s) 1499, 1640, 2055
BsiSI CCGG 8 cut(s) 272, 415, 791, 1346, 1482, 2046, 2077, 2485
BslFI GGGAC 7 cut(s) 945, 1266, 1912, 2075, 2162, 2415, 2426
BslI CCNNNNNNNGG 5 cut(s) 471, 1501, 1576, 1968, 2361
BsmAI GTCTC 5 cut(s) 244, 461, 1427, 1483, 1513
BsmBI CGTCTC 2 cut(s) 1427, 1483
BsmFI GGGAC 7 cut(s) 945, 1266, 1912, 2075, 2162, 2415, 2426
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 7 cut(s) 578, 645, 743, 1580, 1763, 2045, 2301
BsoBI CYCGRG 3 cut(s) 1499, 1640, 2055
Bsp119I TTCGAA 1 cut(s) 1668
Bsp1286I GDGCHC 3 cut(s) 433, 523, 1892
Bsp1407I TGTACA 2 cut(s) 99, 1188
Bsp1720I GCTNAGC 1 cut(s) 528
Bsp19I CCATGG 2 cut(s) 1570, 1827
BspANI GGCC 7 cut(s) 578, 645, 743, 1580, 1763, 2045, 2301
BspCNI CTCAG 3 cut(s) 520, 1419, 1423
BspDI ATCGAT 3 cut(s) 734, 1663, 2154
BspFNI CGCG 2 cut(s) 1679, 2016
BspHI TCATGA 1 cut(s) 1230
BspPI GGATC 2 cut(s) 988, 1001
BspQI GCTCTTC 2 cut(s) 306, 2261
BspT104I TTCGAA 1 cut(s) 1668
BspT107I GGYRCC 2 cut(s) 1971, 2465
BspTI CTTAAG 1 cut(s) 2219
BsrDI GCAATG 1 cut(s) 567
BsrFI RCCGGY 1 cut(s) 2484
BsrGI TGTACA 2 cut(s) 99, 1188
BsrI ACTGG 3 cut(s) 528, 1276, 2403
BssAI RCCGGY 1 cut(s) 2484
BssECI CCNNGG 9 cut(s) 345, 573, 660, 800, 1570, 1827, 1966, 1967, 2054
BssSI CACGAG 1 cut(s) 1367
BssT1I CCWWGG 2 cut(s) 1570, 1827
Bst2BI CACGAG 1 cut(s) 1367
Bst2UI CCWGG 6 cut(s) 329, 575, 688, 983, 1968, 2303
Bst4CI ACNGT 5 cut(s) 97, 346, 1042, 1894, 2284
Bst6I CTCTTC 4 cut(s) 215, 306, 506, 2261
BstAFI CTTAAG 1 cut(s) 2219
BstAUI TGTACA 2 cut(s) 99, 1188
BstBAI YACGTR 4 cut(s) 310, 898, 1202, 2199
BstBI TTCGAA 1 cut(s) 1668
BstC8I GCNNGC 3 cut(s) 20, 24, 706
BstDEI CTNAG 9 cut(s) 355, 434, 528, 1049, 1406, 1431, 1919, 2066, 2326
BstDSI CCRYGG 4 cut(s) 345, 800, 1570, 1827
BstENI CCTNNNNNAGG 1 cut(s) 1499
BstF5I GGATG 7 cut(s) 807, 2046, 2050, 2181, 2212, 2351, 2441
BstFNI CGCG 2 cut(s) 1679, 2016
BstH2I RGCGCY 1 cut(s) 753
BstHHI GCGC 2 cut(s) 28, 752
BstMAI GTCTC 5 cut(s) 244, 461, 1427, 1483, 1513
BstMCI CGRYCG 3 cut(s) 646, 738, 1350
BstMWI GCNNNNNNNGC 3 cut(s) 749, 1482, 1591
BstNI CCWGG 6 cut(s) 329, 575, 688, 983, 1968, 2303
BstNSI RCATGY 3 cut(s) 26, 376, 2124
BstSCI CCNGG 8 cut(s) 327, 414, 573, 686, 981, 1966, 2045, 2301
BstSLI GKGCMC 2 cut(s) 523, 1892
BstSNI TACGTA 2 cut(s) 310, 898
BstUI CGCG 2 cut(s) 1679, 2016
BstV1I GCAGC 6 cut(s) 849, 865, 1965, 2250, 2394, 2470
BstX2I RGATCY 2 cut(s) 143, 993
BstYI RGATCY 2 cut(s) 143, 993
BstZI CGGCCG 1 cut(s) 643
Bsu15I ATCGAT 3 cut(s) 734, 1663, 2154
BsuI GTATCC 3 cut(s) 836, 1218, 1828
BsuRI GGCC 7 cut(s) 578, 645, 743, 1580, 1763, 2045, 2301
BsuTUI ATCGAT 3 cut(s) 734, 1663, 2154
BtgI CCRYGG 4 cut(s) 345, 800, 1570, 1827
BtgZI GCGATG 2 cut(s) 1340, 2139
BtrI CACGTC 1 cut(s) 679
BtsCI GGATG 7 cut(s) 807, 2046, 2050, 2181, 2212, 2351, 2441
BtsIMutI CAGTG 3 cut(s) 102, 521, 1269
Cac8I GCNNGC 3 cut(s) 20, 24, 706
CciI TCATGA 1 cut(s) 1230
CfoI GCGC 2 cut(s) 28, 752
Cfr10I RCCGGY 1 cut(s) 2484
ClaI ATCGAT 3 cut(s) 734, 1663, 2154
CseI GACGC 2 cut(s) 1348, 1465
CspAI ACCGGT 1 cut(s) 2484
DdeI CTNAG 9 cut(s) 355, 434, 528, 1049, 1406, 1431, 1919, 2066, 2326
DrdI GACNNNNNNGTC 1 cut(s) 1286
DseDI GACNNNNNNGTC 1 cut(s) 1286
EaeI YGGCCR 4 cut(s) 643, 1578, 1761, 2043
EagI CGGCCG 1 cut(s) 643
Eam1104I CTCTTC 4 cut(s) 215, 306, 506, 2261
EarI CTCTTC 4 cut(s) 215, 306, 506, 2261
EclXI CGGCCG 1 cut(s) 643
Eco105I TACGTA 2 cut(s) 310, 898
Eco130I CCWWGG 2 cut(s) 1570, 1827
Eco24I GRGCYC 1 cut(s) 433
Eco47I GGWCC 8 cut(s) 269, 932, 979, 1352, 1427, 2003, 2089, 2402
Eco52I CGGCCG 1 cut(s) 643
Eco57I CTGAAG 2 cut(s) 307, 992
Eco72I CACGTG 2 cut(s) 1202, 2199
Eco88I CYCGRG 3 cut(s) 1499, 1640, 2055
EcoNI CCTNNNNNAGG 1 cut(s) 1499
EcoO109I RGGNCCY 5 cut(s) 932, 979, 1427, 2299, 2402
EcoRII CCWGG 6 cut(s) 327, 573, 686, 981, 1966, 2301
EcoT14I CCWWGG 2 cut(s) 1570, 1827
EcoT22I ATGCAT 2 cut(s) 1743, 2035
EcoT38I GRGCYC 1 cut(s) 433
ErhI CCWWGG 2 cut(s) 1570, 1827
Esp3I CGTCTC 2 cut(s) 1427, 1483
FaqI GGGAC 7 cut(s) 945, 1266, 1912, 2075, 2162, 2415, 2426
FauI CCCGC 1 cut(s) 697
FauNDI CATATG 1 cut(s) 1751
FbaI TGATCA 1 cut(s) 156
Fnu4HI GCNGC 8 cut(s) 177, 646, 838, 854, 1954, 2239, 2383, 2459
FokI GGATG 7 cut(s) 794, 2037, 2053, 2188, 2219, 2338, 2428
FriOI GRGCYC 1 cut(s) 433
Fsp4HI GCNGC 8 cut(s) 177, 646, 838, 854, 1954, 2239, 2383, 2459
FspBI CTAG 2 cut(s) 960, 1361
GlaI GCGC 2 cut(s) 27, 751
GluI GCNGC 8 cut(s) 177, 646, 838, 854, 1954, 2239, 2383, 2459
GsaI CCCAGC 3 cut(s) 947, 2275, 2360
GsuI CTGGAG 1 cut(s) 2324
HaeII RGCGCY 1 cut(s) 753
HaeIII GGCC 7 cut(s) 578, 645, 743, 1580, 1763, 2045, 2301
HapII CCGG 8 cut(s) 272, 415, 791, 1346, 1482, 2046, 2077, 2485
HgaI GACGC 2 cut(s) 1348, 1465
HhaI GCGC 2 cut(s) 28, 752
Hin6I GCGC 2 cut(s) 26, 750
HinP1I GCGC 2 cut(s) 26, 750
HincII GTYRAC 3 cut(s) 334, 988, 1861
HindII GTYRAC 3 cut(s) 334, 988, 1861
HinfI GANTC 4 cut(s) 1009, 1254, 1665, 1940
HpaII CCGG 8 cut(s) 272, 415, 791, 1346, 1482, 2046, 2077, 2485
HphI GGTGA 2 cut(s) 583, 1004
Hpy166II GTNNAC 6 cut(s) 100, 334, 988, 1095, 1861, 1890
Hpy188III TCNNGA 6 cut(s) 62, 1006, 1231, 1408, 1944, 2189
Hpy8I GTNNAC 6 cut(s) 100, 334, 988, 1095, 1861, 1890
Hpy99I CGWCG 6 cut(s) 680, 1046, 1519, 2147, 2195, 2396
HpyAV CCTTC 7 cut(s) 59, 206, 568, 970, 1012, 1025, 1846
HpyCH4III ACNGT 5 cut(s) 97, 346, 1042, 1894, 2284
HpyCH4IV ACGT 9 cut(s) 309, 678, 787, 897, 1201, 1304, 1695, 2027, 2198
HpyF10VI GCNNNNNNNGC 3 cut(s) 749, 1482, 1591
HpyF3I CTNAG 9 cut(s) 355, 434, 528, 1049, 1406, 1431, 1919, 2066, 2326
HpySE526I ACGT 9 cut(s) 309, 678, 787, 897, 1201, 1304, 1695, 2027, 2198
HspAI GCGC 2 cut(s) 26, 750
KflI GGGWCCC 1 cut(s) 932
KpnI GGTACC 2 cut(s) 1975, 2469
Ksp22I TGATCA 1 cut(s) 156
LguI GCTCTTC 2 cut(s) 306, 2261
LmnI GCTCC 2 cut(s) 630, 1438
Lsp1109I GCAGC 6 cut(s) 849, 865, 1965, 2250, 2394, 2470
LweI GCATC 7 cut(s) 1011, 1545, 1623, 2059, 2166, 2194, 2217
MaeI CTAG 2 cut(s) 960, 1361
MaeII ACGT 9 cut(s) 309, 678, 787, 897, 1201, 1304, 1695, 2027, 2198
MaeIII GTNAC 4 cut(s) 902, 1010, 1197, 2489
MflI RGATCY 2 cut(s) 143, 993
MhlI GDGCHC 3 cut(s) 433, 523, 1892
MlsI TGGCCA 1 cut(s) 1763
MluNI TGGCCA 1 cut(s) 1763
MlyI GAGTC 3 cut(s) 1018, 1248, 1949
MmeI TCCRAC 5 cut(s) 190, 699, 1330, 1538, 2153
Mox20I TGGCCA 1 cut(s) 1763
Mph1103I ATGCAT 2 cut(s) 1743, 2035
MroXI GAANNNNTTC 1 cut(s) 1696
MscI TGGCCA 1 cut(s) 1763
MseI TTAA 8 cut(s) 1037, 1073, 1247, 1334, 2024, 2061, 2220, 2436
MslI CAYNNNNRTG 3 cut(s) 95, 2201, 2487
Msp20I TGGCCA 1 cut(s) 1763
MspA1I CMGCKG 2 cut(s) 840, 1956
MspCI CTTAAG 1 cut(s) 2219
MspI CCGG 8 cut(s) 272, 415, 791, 1346, 1482, 2046, 2077, 2485
MspR9I CCNGG 8 cut(s) 329, 416, 575, 688, 983, 1968, 2047, 2303
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 6 cut(s) 329, 575, 688, 983, 1968, 2303
MvnI CGCG 2 cut(s) 1679, 2016
MwoI GCNNNNNNNGC 3 cut(s) 749, 1482, 1591
NciI CCSGG 2 cut(s) 416, 2047
NcoI CCATGG 2 cut(s) 1570, 1827
NdeI CATATG 1 cut(s) 1751
NmeAIII GCCGAG 2 cut(s) 641, 1245
NmuCI GTSAC 4 cut(s) 902, 1010, 1197, 2489
NsiI ATGCAT 2 cut(s) 1743, 2035
NspI RCATGY 3 cut(s) 26, 376, 2124
NspV TTCGAA 1 cut(s) 1668
OliI CACNNNNGTG 1 cut(s) 2487
PaeI GCATGC 1 cut(s) 26
PaeR7I CTCGAG 1 cut(s) 1499
PagI TCATGA 1 cut(s) 1230
PasI CCCWGGG 1 cut(s) 1967
PciI ACATGT 1 cut(s) 372
PciSI GCTCTTC 2 cut(s) 306, 2261
PcsI WCGNNNNNNNCGW 2 cut(s) 1497, 2151
PctI GAATGC 1 cut(s) 275
PdmI GAANNNNTTC 1 cut(s) 1696
PfeI GAWTC 1 cut(s) 1665
PinAI ACCGGT 1 cut(s) 2484
PkrI GCNGC 8 cut(s) 178, 647, 839, 855, 1955, 2240, 2384, 2460
Ple19I CGATCG 1 cut(s) 738
PleI GAGTC 3 cut(s) 1017, 1248, 1948
PmaCI CACGTG 2 cut(s) 1202, 2199
PmlI CACGTG 2 cut(s) 1202, 2199
PpsI GAGTC 3 cut(s) 1017, 1248, 1948
Ppu21I YACGTR 4 cut(s) 310, 898, 1202, 2199
PpuMI RGGWCCY 4 cut(s) 932, 979, 1427, 2402
PscI ACATGT 1 cut(s) 372
PsiI TTATAA 2 cut(s) 87, 1790
Psp1406I AACGTT 2 cut(s) 1304, 1695
Psp5II RGGWCCY 4 cut(s) 932, 979, 1427, 2402
Psp6I CCWGG 6 cut(s) 327, 573, 686, 981, 1966, 2301
PspCI CACGTG 2 cut(s) 1202, 2199
PspFI CCCAGC 3 cut(s) 943, 2271, 2356
PspGI CCWGG 6 cut(s) 327, 573, 686, 981, 1966, 2301
PspPPI RGGWCCY 4 cut(s) 932, 979, 1427, 2402
PspXI VCTCGAGB 1 cut(s) 1499
PsuI RGATCY 2 cut(s) 143, 993
PvuI CGATCG 1 cut(s) 738
RseI CAYNNNNRTG 3 cut(s) 95, 2201, 2487
SapI GCTCTTC 2 cut(s) 306, 2261
SaqAI TTAA 8 cut(s) 1037, 1073, 1247, 1334, 2024, 2061, 2220, 2436
SatI GCNGC 8 cut(s) 177, 646, 838, 854, 1954, 2239, 2383, 2459
SchI GAGTC 3 cut(s) 1018, 1248, 1949
ScrFI CCNGG 8 cut(s) 329, 416, 575, 688, 983, 1968, 2047, 2303
SduI GDGCHC 3 cut(s) 433, 523, 1892
SfaNI GCATC 7 cut(s) 1011, 1545, 1623, 2059, 2166, 2194, 2217
Sfr274I CTCGAG 1 cut(s) 1499
SfuI TTCGAA 1 cut(s) 1668
SgrAI CRCCGGYG 1 cut(s) 2484
SinI GGWCC 8 cut(s) 269, 932, 979, 1352, 1427, 2003, 2089, 2402
SlaI CTCGAG 1 cut(s) 1499
SmiMI CAYNNNNRTG 3 cut(s) 95, 2201, 2487
SmlI CTYRAG 4 cut(s) 395, 1468, 1499, 2219
SmoI CTYRAG 4 cut(s) 395, 1468, 1499, 2219
SnaBI TACGTA 2 cut(s) 310, 898
SphI GCATGC 1 cut(s) 26
SspI AATATT 1 cut(s) 2290
SspMI CTAG 2 cut(s) 960, 1361
StyD4I CCNGG 8 cut(s) 327, 414, 573, 686, 981, 1966, 2045, 2301
StyI CCWWGG 2 cut(s) 1570, 1827
TaaI ACNGT 5 cut(s) 97, 346, 1042, 1894, 2284
TaiI ACGT 9 cut(s) 312, 681, 790, 900, 1204, 1307, 1698, 2030, 2201
TaqI TCGA 8 cut(s) 734, 1491, 1500, 1663, 1668, 2154, 2190, 2225
TatI WGTACW 6 cut(s) 99, 131, 781, 1188, 1644, 2322
TauI GCSGC 2 cut(s) 179, 648
TfiI GAWTC 1 cut(s) 1665
Tru1I TTAA 8 cut(s) 1037, 1073, 1247, 1334, 2024, 2061, 2220, 2436
Tru9I TTAA 8 cut(s) 1037, 1073, 1247, 1334, 2024, 2061, 2220, 2436
TscAI CASTG 3 cut(s) 102, 528, 1276
TseFI GTSAC 4 cut(s) 902, 1010, 1197, 2489
TseI GCWGC 6 cut(s) 837, 853, 1953, 2238, 2382, 2458
Tsp45I GTSAC 4 cut(s) 902, 1010, 1197, 2489
TspGWI ACGGA 3 cut(s) 1451, 1836, 2434
TspRI CASTG 3 cut(s) 102, 528, 1276
Vha464I CTTAAG 1 cut(s) 2219
VneI GTGCAC 1 cut(s) 1888
VpaK11BI GGWCC 8 cut(s) 269, 932, 979, 1352, 1427, 2003, 2089, 2402
XagI CCTNNNNNAGG 1 cut(s) 1499
XapI RAATTY 3 cut(s) 1158, 1879, 2114
XceI RCATGY 3 cut(s) 26, 376, 2124
XcmI CCANNNNNNNNNTGG 1 cut(s) 1246
XhoI CTCGAG 1 cut(s) 1499
XmnI GAANNNNTTC 1 cut(s) 1696
XspI CTAG 2 cut(s) 960, 1361
Zsp2I ATGCAT 2 cut(s) 1743, 2035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.