MD02G1285000.v1.1

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001291
Physical Location & Seq
Reverse (-)
34142711 .. 34145420
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1285000.v1.1.491

Sequence Viewer

Length: 2499 bp
ATGTCTTATTTTAAGTTGACTGGGAGTGTTGGTGGGGAAGATTTTGTAATATCCAGTAACACAAAAGATTTAATTGTGGTATGCTTCAATAAAACGATATCCCCAACAGGACTATGGCGAGCACACAACCCCTTTATGTCAGCTCTTTCAGTTCTTGTTATAAAACTGAGCATTGCCGTCTTCAGCACTCGTATTTTTATGGTCGCCTTAAAACCCTTGCATCAACCACGCATTGTTGCTGAGATTCTTGGTGGAATGTTGACGAGTACGAGTAATTGGGGTAGAGTTGTACCAGCCGTATTTCCTTACAGTACCGTGAAGATTATCGAGACCATCGGAAACTTGGCCCTCGTTTACCACATGTTCCTGGTGGGTTTAGAGCTTGATTTAAAACCAGTTTTACGAGCTGGAAAGAAAGCTCTTAGCATTGCGCTCGCCGGCATTGTCTTTTCCGTCCTTGTTGGTTGGGCCTTATGTCGATATGTGCTCCTCAAAGATTTTGTCGAAGAAACAAAAGGCAAGGATGGACAAAAATTTCCCACTGTGAACGGCCCGTGGTTCTGGGGCGTTGCTCTTGCCACCACCAACTTCCCAGACCTTGCCCGAATCCTCACAGACCTCAAACTTCTCTACTCTGCTGTCGGAGGACTTGCGTTATCTGCCTCTGTTATCTCCGACTTATGCTCCTGGTTTCTTCTTTTGCTCGTAATGGCCATAGTCAACAACTTCCAGACAATTTCGGTGGGACTCACCTTGCTTTTTATAGGGCTATGCATTTTTATCGTACGTCCTCTTCTGTCACAGATTGTTGACCGAATTCGTGACAAAGTACAAGACGATGGCGACTACGGTCAACTTGAATGCTTTGTTCTAGCCGGGGTGGTGCTTTGTGGGTACATTACCGATGCATGTGGGTCTCATTCTATCGTAGGGTCTTTTATGTTTGGAGCTATCATGCCTAGAGGAGAGTTTTCAAACAATCTTAATAAGAAGGTGGGAAGTTTTGTGCCTGGGATTCTGTTGCCTCTCTACTTCTGTATCAATGGGAGTAGACTCAACTATGTGGATATTCTCAGTTCCGTTTATCCATCGGAGAAAAAAACTGGGACGGACATATATCGTGTAGGGGGGGTTGTCATCTTAGCTTTTGCAGCTAAGACACTGAGCACTTTGGTTGCCGGCTTAGTAAACAAAATGTCATTTCGGGATAGTTTGGCTCTTGGAGTGCTCATGAACACCAAGGGCTTATTGACACTTATTATACTCAACACCGGTCGGGAAGTAAAGGCGTTGAACAAACAAACCTTCGGAGTAATGGTGCTGGCAATTTGGGTAATGACATTTGTAGTTGGTCCGCTTCTAGCAATTTTTTACAAGTCCAGTGCTAGGCCTTTCGTGCAATACAAAGAGAGAAATGTAGTAAGCGTAGGATCTAACAACGAGCTTCGAATCCTTGCATGTGCTCACAACTCACGCAATGTGCCAGGCATTATCAACCTCCTCGAGGCTTCTAATCCAACCAATAAATCCCCTCTTCATGTTCTTGCTGTCCACCTTGTGGAACTAACGGGGCATACTTCGGCGATGTTGGTAGTGCACGATGCTTGTAAGACCAATGATGTATATTCTCCCGACAACTCGTCCCCCTCAAATGCTTTTGAAGCATATGCCCAACAAAGAGAATGTGTCACGATGCAAACACTCACGGCGGTGTCTCAATACTCCACGATGCACCAGGATATATGCAACCTGGCTGAGGAGAATCGCGTCGCCATAATAATCATCCCGTTCCACAATAAATCCACCACATTGGTTGGAGGAGTGGCACCAGATGCAAACAACTCACATCTAAAGAGCCTCAACAACAACTTGATCGCAAATGCTCGGTGCTCTGTGGGTGTTTTTGTGGATCGAGGCCTCGGTGCATCCAACGACTCTTACCGTCGATGCTGCTATTCCATGCTCTTCTTTGGAGGGGTAGATGACCGTGAGGCATTAGCGTATGCGGGTCGCATGGCTGGACATCCTAGGACTAGCCTAACAGTCATATCGTTTAACATCATGAGTAAAAATGCATCAAACTTGTGCTCTGAGGATGATCATGGTGATGATGATGACGAAGACGATGACGATGAAGATGGTACTGGTGGAATTCTGAAGGCAATGGCAAGCACTGGGAGGGAAAAAAAACTGGATGAGTTGTACTTGGATGAGTTTAGGCTGATGTCAATGAATGATGCCTCCATAAAACTTTTAGAGAAATCGGTGACTAGGTGGGAACAAATTCTCTCACTAATAAGCTCAATGGAAGGTGAGTATGATATGTACATAGTAGGAAGAAGGCATGGAAATATATCAGAGGTCACCACGACGTTACTCGATGGCATCGACTCCAATGACCTTGGATTTCTTGGAGATGCATTGGTATCTTCAAACCTTACAGCAATTACATCCGTTCTAGTTGTGCAACAAGGTCGCCTTTTGGATTATTATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

833

Amino Acids

90.7

Weight (kDa)

6.23

Isoelectric Point (pI)

30.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 64 - 456 5.7e-47 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 512 - 643 7.6e-16 Plant cation/H(+) antiporter domain
CHX17_C PF23259 653 - 684 1.8e-06 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 161
AbsI CCTCGAGG 1 cut(s) 1502
AccB1I GGYRCC 1 cut(s) 1825
AccB7I CCANNNNNTGG 1 cut(s) 1558
AccI GTMKAC 1 cut(s) 1051
AccII CGCG 1 cut(s) 1767
AciI CCGC 3 cut(s) 1355, 1709, 2006
AclWI GGATC 2 cut(s) 1438, 1917
AcoI YGGCCR 1 cut(s) 711
AcsI RAATTY 4 cut(s) 533, 816, 2151, 2283
AcuI CTGAAG 2 cut(s) 166, 2177
AdeI CACNNNGTG 1 cut(s) 1558
AfaI GTAC 9 cut(s) 268, 291, 313, 786, 831, 896, 2143, 2204, 2327
AfiI CCNNNNNNNGG 4 cut(s) 1386, 1504, 1558, 1756
AflIII ACRYGT 1 cut(s) 360
AgeI ACCGGT 1 cut(s) 1271
AgsI TTSAA 6 cut(s) 88, 860, 975, 1294, 1661, 2433
AhdI GACNNNNNGTC 1 cut(s) 1639
AjnI CCWGG 6 cut(s) 366, 686, 1009, 1483, 1734, 1749
AleI CACNNNNGTG 1 cut(s) 1709
AluBI AGCT 9 cut(s) 143, 382, 407, 419, 950, 1145, 1154, 1444, 2301
AluI AGCT 9 cut(s) 143, 382, 407, 419, 950, 1145, 1154, 1444, 2301
Alw21I GWGCWC 8 cut(s) 124, 489, 1169, 1230, 1465, 1599, 1892, 2090
Alw26I GTCTC 3 cut(s) 323, 921, 1719
Alw44I GTGCAC 1 cut(s) 1595
AlwI GGATC 2 cut(s) 1438, 1917
Ama87I CYCGRG 1 cut(s) 1502
AoxI GGCC 6 cut(s) 345, 468, 550, 711, 1388, 1915
ApaLI GTGCAC 1 cut(s) 1595
ApeKI GCWGC 2 cut(s) 1151, 1950
ApoI RAATTY 4 cut(s) 533, 816, 2151, 2283
ArsI GACNNNNNNTTYG 2 cut(s) 486, 518
AsiGI ACCGGT 1 cut(s) 1271
Asp700I GAANNNNTTC 1 cut(s) 2283
AspA2I CCTAGG 1 cut(s) 2027
AspLEI GCGC 1 cut(s) 433
AspS9I GGNCC 4 cut(s) 346, 468, 551, 1352
AsuC2I CCSGG 1 cut(s) 877
AsuHPI GGTGA 5 cut(s) 742, 2117, 2278, 2324, 2356
AsuII TTCGAA 1 cut(s) 1447
AvaI CYCGRG 1 cut(s) 1502
AvaII GGWCC 1 cut(s) 1352
AvrII CCTAGG 1 cut(s) 2027
BaeGI GKGCMC 1 cut(s) 1599
BaeI ACNNNNGTAYC 2 cut(s) 1021, 1054
BalI TGGCCA 1 cut(s) 713
BanI GGYRCC 1 cut(s) 1825
BarI GAAGNNNNNNTAC 2 cut(s) 777, 809
BbsI GAAGAC 2 cut(s) 172, 2127
Bbv12I GWGCWC 8 cut(s) 124, 489, 1169, 1230, 1465, 1599, 1892, 2090
BbvCI CCTCAGC 1 cut(s) 1755
BbvI GCAGC 2 cut(s) 1163, 1937
BccI CCATC 6 cut(s) 341, 518, 833, 1096, 2132, 2375
BceAI ACGGC 4 cut(s) 161, 281, 565, 1722
BcgI CGANNNNNNTGC 6 cut(s) 415, 449, 763, 797, 2456, 2490
BciT130I CCWGG 6 cut(s) 368, 688, 1011, 1485, 1736, 1751
BclI TGATCA 1 cut(s) 2098
BcnI CCSGG 1 cut(s) 877
BcoDI GTCTC 3 cut(s) 323, 921, 1719
BfaI CTAG 8 cut(s) 872, 960, 1361, 1386, 2028, 2034, 2271, 2459
BisI GCNGC 2 cut(s) 1152, 1951
BlnI CCTAGG 1 cut(s) 2027
BlsI GCNGC 2 cut(s) 1153, 1952
Bme1390I CCNGG 7 cut(s) 368, 688, 877, 1011, 1485, 1736, 1751
Bme18I GGWCC 1 cut(s) 1352
BmeRI GACNNNNNGTC 1 cut(s) 1639
BmeT110I CYCGRG 1 cut(s) 1502
BmgT120I GGNCC 4 cut(s) 346, 468, 551, 1352
BmiI GGNNCC 1 cut(s) 1827
BmrFI CCNGG 7 cut(s) 368, 688, 877, 1011, 1485, 1736, 1751
BmrI ACTGGG 3 cut(s) 30, 1113, 2184
BmuI ACTGGG 3 cut(s) 30, 1113, 2184
BoxI GACNNNNGTC 1 cut(s) 849
BpiI GAAGAC 2 cut(s) 172, 2127
Bpu10I CCTNAGC 1 cut(s) 1755
Bpu14I TTCGAA 1 cut(s) 1447
BpuMI CCSGG 1 cut(s) 877
BsaI GGTCTC 2 cut(s) 323, 921
BsaJI CCNNGG 7 cut(s) 554, 876, 1010, 1239, 1918, 2027, 2401
BsaWI WCCGGW 1 cut(s) 1271
BsaXI ACNNNNNCTCC 6 cut(s) 16, 46, 668, 698, 1302, 1332
Bsc4I CCNNNNNNNGG 4 cut(s) 1386, 1504, 1558, 1756
Bse118I RCCGGY 3 cut(s) 437, 1178, 1271
Bse1I ACTGG 8 cut(s) 25, 54, 395, 1108, 1380, 2149, 2179, 2196
Bse3DI GCAATG 4 cut(s) 171, 426, 1483, 2169
BseBI CCWGG 6 cut(s) 368, 688, 1011, 1485, 1736, 1751
BseDI CCNNGG 7 cut(s) 554, 876, 1010, 1239, 1918, 2027, 2401
BseGI GGATG 8 cut(s) 529, 1782, 1925, 2023, 2101, 2200, 2215, 2450
BseLI CCNNNNNNNGG 4 cut(s) 1386, 1504, 1558, 1756
BseMI GCAATG 4 cut(s) 171, 426, 1483, 2169
BseMII CTCAG 6 cut(s) 158, 231, 1087, 1154, 1746, 2082
BseNI ACTGG 8 cut(s) 25, 54, 395, 1108, 1380, 2149, 2179, 2196
BseRI GAGGAG 5 cut(s) 479, 978, 1490, 1772, 1833
BseSI GKGCMC 1 cut(s) 1599
BseXI GCAGC 2 cut(s) 1163, 1937
Bsh1236I CGCG 1 cut(s) 1767
Bsh1285I CGRYCG 1 cut(s) 1276
BshFI GGCC 6 cut(s) 347, 470, 552, 713, 1390, 1917
BshNI GGYRCC 1 cut(s) 1825
BshTI ACCGGT 1 cut(s) 1271
BsiEI CGRYCG 1 cut(s) 1276
BsiHKAI GWGCWC 8 cut(s) 124, 489, 1169, 1230, 1465, 1599, 1892, 2090
BsiHKCI CYCGRG 1 cut(s) 1502
BsiSI CCGG 4 cut(s) 438, 876, 1179, 1272
BsiWI CGTACG 1 cut(s) 784
BslFI GGGAC 3 cut(s) 759, 1120, 1627
BslI CCNNNNNNNGG 4 cut(s) 1386, 1504, 1558, 1756
BsmAI GTCTC 3 cut(s) 323, 921, 1719
BsmFI GGGAC 3 cut(s) 759, 1120, 1627
BsmI GAATGC 1 cut(s) 866
BsnI GGCC 6 cut(s) 347, 470, 552, 713, 1390, 1917
Bso31I GGTCTC 2 cut(s) 323, 921
BsoBI CYCGRG 1 cut(s) 1502
Bsp119I TTCGAA 1 cut(s) 1447
Bsp1286I GDGCHC 8 cut(s) 124, 489, 1169, 1230, 1465, 1599, 1892, 2090
Bsp1407I TGTACA 1 cut(s) 2325
Bsp143I GATC 4 cut(s) 1430, 1872, 1909, 2098
BspACI CCGC 3 cut(s) 1355, 1709, 2006
BspANI GGCC 6 cut(s) 347, 470, 552, 713, 1390, 1917
BspCNI CTCAG 6 cut(s) 159, 232, 1086, 1155, 1747, 2083
BspFNI CGCG 1 cut(s) 1767
BspHI TCATGA 2 cut(s) 1230, 2061
BspLI GGNNCC 1 cut(s) 1827
BspPI GGATC 2 cut(s) 1438, 1917
BspQI GCTCTTC 1 cut(s) 1970
BspT104I TTCGAA 1 cut(s) 1447
BspT107I GGYRCC 1 cut(s) 1825
BspTNI GGTCTC 2 cut(s) 323, 921
BsrDI GCAATG 4 cut(s) 171, 426, 1483, 2169
BsrFI RCCGGY 3 cut(s) 437, 1178, 1271
BsrGI TGTACA 1 cut(s) 2325
BsrI ACTGG 8 cut(s) 25, 54, 395, 1108, 1380, 2149, 2179, 2196
BssAI RCCGGY 3 cut(s) 437, 1178, 1271
BssECI CCNNGG 7 cut(s) 554, 876, 1010, 1239, 1918, 2027, 2401
BssMI GATC 4 cut(s) 1430, 1872, 1909, 2098
BssT1I CCWWGG 3 cut(s) 1239, 2027, 2401
Bst2UI CCWGG 6 cut(s) 368, 688, 1011, 1485, 1736, 1751
Bst4CI ACNGT 7 cut(s) 311, 316, 544, 851, 1943, 1988, 2044
Bst6I CTCTTC 3 cut(s) 798, 1539, 1970
BstAPI GCANNNNNTGC 1 cut(s) 1832
BstAUI TGTACA 1 cut(s) 2325
BstBI TTCGAA 1 cut(s) 1447
BstC8I GCNNGC 6 cut(s) 120, 435, 439, 1180, 1323, 2170
BstDSI CCRYGG 1 cut(s) 554
BstEII GGTNACC 1 cut(s) 2362
BstENI CCTNNNNNAGG 2 cut(s) 1502, 1754
BstF5I GGATG 8 cut(s) 529, 1782, 1925, 2023, 2101, 2200, 2215, 2450
BstFNI CGCG 1 cut(s) 1767
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 4 cut(s) 1433, 1875, 1912, 2101
BstMAI GTCTC 3 cut(s) 323, 921, 1719
BstMBI GATC 4 cut(s) 1430, 1872, 1909, 2098
BstMCI CGRYCG 1 cut(s) 1276
BstMWI GCNNNNNNNGC 5 cut(s) 659, 1151, 1396, 1661, 1832
BstNI CCWGG 6 cut(s) 368, 688, 1011, 1485, 1736, 1751
BstNSI RCATGY 3 cut(s) 364, 912, 1461
BstPAI GACNNNNGTC 1 cut(s) 849
BstPI GGTNACC 1 cut(s) 2362
BstSCI CCNGG 7 cut(s) 366, 686, 875, 1009, 1483, 1734, 1749
BstSLI GKGCMC 1 cut(s) 1599
BstUI CGCG 1 cut(s) 1767
BstV1I GCAGC 2 cut(s) 1163, 1937
BstV2I GAAGAC 2 cut(s) 172, 2127
BstX2I RGATCY 1 cut(s) 1430
BstXI CCANNNNNNTGG 1 cut(s) 1810
BstYI RGATCY 1 cut(s) 1430
BsuRI GGCC 6 cut(s) 347, 470, 552, 713, 1390, 1917
BtgI CCRYGG 1 cut(s) 554
BtgZI GCGATG 1 cut(s) 1598
BtsCI GGATG 8 cut(s) 529, 1782, 1925, 2023, 2101, 2200, 2215, 2450
BtsIMutI CAGTG 4 cut(s) 540, 1160, 1387, 2172
Cac8I GCNNGC 6 cut(s) 120, 435, 439, 1180, 1323, 2170
CciI TCATGA 2 cut(s) 1230, 2061
CfoI GCGC 1 cut(s) 433
Cfr10I RCCGGY 3 cut(s) 437, 1178, 1271
Cfr13I GGNCC 4 cut(s) 346, 468, 551, 1352
CseI GACGC 1 cut(s) 1756
Csp6I GTAC 9 cut(s) 267, 290, 312, 785, 830, 895, 2142, 2203, 2326
CspAI ACCGGT 1 cut(s) 1271
CspCI CAANNNNNGTGG 4 cut(s) 723, 758, 1795, 1830
CviQI GTAC 9 cut(s) 267, 290, 312, 785, 830, 895, 2142, 2203, 2326
DpnI GATC 4 cut(s) 1432, 1874, 1911, 2100
DpnII GATC 4 cut(s) 1430, 1872, 1909, 2098
DraI TTTAAA 1 cut(s) 390
DraIII CACNNNGTG 1 cut(s) 1558
DriI GACNNNNNGTC 1 cut(s) 1639
EaeI YGGCCR 1 cut(s) 711
Eam1104I CTCTTC 3 cut(s) 798, 1539, 1970
Eam1105I GACNNNNNGTC 1 cut(s) 1639
EarI CTCTTC 3 cut(s) 798, 1539, 1970
Eco130I CCWWGG 3 cut(s) 1239, 2027, 2401
Eco147I AGGCCT 2 cut(s) 1390, 1917
Eco31I GGTCTC 2 cut(s) 323, 921
Eco32I GATATC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 1352
Eco57I CTGAAG 2 cut(s) 166, 2177
Eco88I CYCGRG 1 cut(s) 1502
Eco91I GGTNACC 1 cut(s) 2362
EcoNI CCTNNNNNAGG 2 cut(s) 1502, 1754
EcoO65I GGTNACC 1 cut(s) 2362
EcoRI GAATTC 2 cut(s) 816, 2151
EcoRII CCWGG 6 cut(s) 366, 686, 1009, 1483, 1734, 1749
EcoRV GATATC 1 cut(s) 99
EcoT14I CCWWGG 3 cut(s) 1239, 2027, 2401
EcoT22I ATGCAT 4 cut(s) 776, 910, 2077, 2422
ErhI CCWWGG 3 cut(s) 1239, 2027, 2401
FalI AAGNNNNNCTT 2 cut(s) 2463, 2495
FaqI GGGAC 3 cut(s) 759, 1120, 1627
FauI CCCGC 1 cut(s) 1999
FauNDI CATATG 1 cut(s) 1666
FbaI TGATCA 1 cut(s) 2098
FblI GTMKAC 1 cut(s) 1051
Fnu4HI GCNGC 2 cut(s) 1152, 1951
FokI GGATG 8 cut(s) 536, 1769, 1912, 2010, 2108, 2207, 2222, 2437
Fsp4HI GCNGC 2 cut(s) 1152, 1951
FspBI CTAG 8 cut(s) 872, 960, 1361, 1386, 2028, 2034, 2271, 2459
GlaI GCGC 1 cut(s) 432
GluI GCNGC 2 cut(s) 1152, 1951
HaeIII GGCC 6 cut(s) 347, 470, 552, 713, 1390, 1917
HapII CCGG 4 cut(s) 438, 876, 1179, 1272
HgaI GACGC 1 cut(s) 1756
HhaI GCGC 1 cut(s) 433
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HincII GTYRAC 5 cut(s) 18, 261, 721, 811, 854
HindII GTYRAC 5 cut(s) 18, 261, 721, 811, 854
HinfI GANTC 9 cut(s) 244, 606, 747, 1015, 1053, 1449, 1762, 1934, 2390
HpaII CCGG 4 cut(s) 438, 876, 1179, 1272
HphI GGTGA 5 cut(s) 742, 2117, 2278, 2324, 2356
Hpy188I TCNGA 8 cut(s) 338, 644, 676, 1093, 1310, 2092, 2157, 2359
Hpy99I CGWCG 3 cut(s) 1772, 1947, 2374
HpyAV CCTTC 5 cut(s) 985, 1315, 2152, 2303, 2334
HpyCH4III ACNGT 7 cut(s) 311, 316, 544, 851, 1943, 1988, 2044
HpyCH4IV ACGT 2 cut(s) 787, 2372
HpyF10VI GCNNNNNNNGC 5 cut(s) 659, 1151, 1396, 1661, 1832
HpySE526I ACGT 2 cut(s) 787, 2372
HspAI GCGC 1 cut(s) 431
KroI GCCGGC 2 cut(s) 437, 1178
KroNI GCCGGC 2 cut(s) 439, 1180
Ksp22I TGATCA 1 cut(s) 2098
Kzo9I GATC 4 cut(s) 1430, 1872, 1909, 2098
LguI GCTCTTC 1 cut(s) 1970
LmnI GCTCC 3 cut(s) 492, 689, 947
Lsp1109I GCAGC 2 cut(s) 1163, 1937
MaeI CTAG 8 cut(s) 872, 960, 1361, 1386, 2028, 2034, 2271, 2459
MaeII ACGT 2 cut(s) 787, 2372
MaeIII GTNAC 7 cut(s) 56, 798, 821, 1687, 2266, 2362, 2373
MalI GATC 4 cut(s) 1432, 1874, 1911, 2100
MboI GATC 4 cut(s) 1430, 1872, 1909, 2098
MflI RGATCY 1 cut(s) 1430
MhlI GDGCHC 8 cut(s) 124, 489, 1169, 1230, 1465, 1599, 1892, 2090
MlsI TGGCCA 1 cut(s) 713
MluNI TGGCCA 1 cut(s) 713
MlyI GAGTC 4 cut(s) 741, 1047, 1928, 2384
MmeI TCCRAC 5 cut(s) 622, 699, 1541, 1795, 1953
Mox20I TGGCCA 1 cut(s) 713
Mph1103I ATGCAT 4 cut(s) 776, 910, 2077, 2422
MroNI GCCGGC 2 cut(s) 437, 1178
MroXI GAANNNNTTC 1 cut(s) 2283
MscI TGGCCA 1 cut(s) 713
MseI TTAA 6 cut(s) 12, 71, 209, 389, 984, 2055
MslI CAYNNNNRTG 2 cut(s) 1709, 2106
Msp20I TGGCCA 1 cut(s) 713
MspI CCGG 4 cut(s) 438, 876, 1179, 1272
MspR9I CCNGG 7 cut(s) 368, 688, 877, 1011, 1485, 1736, 1751
Mva1269I GAATGC 1 cut(s) 866
MvaI CCWGG 6 cut(s) 368, 688, 1011, 1485, 1736, 1751
MvnI CGCG 1 cut(s) 1767
MwoI GCNNNNNNNGC 5 cut(s) 659, 1151, 1396, 1661, 1832
NaeI GCCGGC 2 cut(s) 439, 1180
NciI CCSGG 1 cut(s) 877
NdeI CATATG 1 cut(s) 1666
NdeII GATC 4 cut(s) 1430, 1872, 1909, 2098
NgoMIV GCCGGC 2 cut(s) 437, 1178
NlaIV GGNNCC 1 cut(s) 1827
NmuCI GTSAC 5 cut(s) 798, 821, 1687, 2266, 2362
NsiI ATGCAT 4 cut(s) 776, 910, 2077, 2422
NspI RCATGY 3 cut(s) 364, 912, 1461
NspV TTCGAA 1 cut(s) 1447
OliI CACNNNNGTG 1 cut(s) 1709
PaeR7I CTCGAG 1 cut(s) 1502
PagI TCATGA 2 cut(s) 1230, 2061
PceI AGGCCT 2 cut(s) 1390, 1917
PciI ACATGT 1 cut(s) 360
PciSI GCTCTTC 1 cut(s) 1970
PcsI WCGNNNNNNNCGW 1 cut(s) 2385
PctI GAATGC 1 cut(s) 866
PdiI GCCGGC 2 cut(s) 439, 1180
PdmI GAANNNNTTC 1 cut(s) 2283
PfeI GAWTC 5 cut(s) 244, 606, 1015, 1449, 1762
Pfl23II CGTACG 1 cut(s) 784
PflMI CCANNNNNTGG 1 cut(s) 1558
PinAI ACCGGT 1 cut(s) 1271
PkrI GCNGC 2 cut(s) 1153, 1952
PleI GAGTC 4 cut(s) 741, 1047, 1928, 2384
PpsI GAGTC 4 cut(s) 741, 1047, 1928, 2384
PscI ACATGT 1 cut(s) 360
PshAI GACNNNNGTC 1 cut(s) 849
PsiI TTATAA 1 cut(s) 161
Psp6I CCWGG 6 cut(s) 366, 686, 1009, 1483, 1734, 1749
PspEI GGTNACC 1 cut(s) 2362
PspGI CCWGG 6 cut(s) 366, 686, 1009, 1483, 1734, 1749
PspLI CGTACG 1 cut(s) 784
PspN4I GGNNCC 1 cut(s) 1827
PspPI GGNCC 4 cut(s) 346, 468, 551, 1352
PspXI VCTCGAGB 1 cut(s) 1502
PsuI RGATCY 1 cut(s) 1430
RsaI GTAC 9 cut(s) 268, 291, 313, 786, 831, 896, 2143, 2204, 2327
RsaNI GTAC 9 cut(s) 267, 290, 312, 785, 830, 895, 2142, 2203, 2326
RseI CAYNNNNRTG 2 cut(s) 1709, 2106
SapI GCTCTTC 1 cut(s) 1970
SaqAI TTAA 6 cut(s) 12, 71, 209, 389, 984, 2055
SatI GCNGC 2 cut(s) 1152, 1951
Sau3AI GATC 4 cut(s) 1430, 1872, 1909, 2098
Sau96I GGNCC 4 cut(s) 346, 468, 551, 1352
SchI GAGTC 4 cut(s) 741, 1047, 1928, 2384
ScrFI CCNGG 7 cut(s) 368, 688, 877, 1011, 1485, 1736, 1751
SduI GDGCHC 8 cut(s) 124, 489, 1169, 1230, 1465, 1599, 1892, 2090
Sfr274I CTCGAG 1 cut(s) 1502
SfuI TTCGAA 1 cut(s) 1447
SinI GGWCC 1 cut(s) 1352
SlaI CTCGAG 1 cut(s) 1502
SmiMI CAYNNNNRTG 2 cut(s) 1709, 2106
SmlI CTYRAG 1 cut(s) 1502
SmoI CTYRAG 1 cut(s) 1502
SseBI AGGCCT 2 cut(s) 1390, 1917
SsiI CCGC 3 cut(s) 1355, 1709, 2006
SspMI CTAG 8 cut(s) 872, 960, 1361, 1386, 2028, 2034, 2271, 2459
StuI AGGCCT 2 cut(s) 1390, 1917
StyD4I CCNGG 7 cut(s) 366, 686, 875, 1009, 1483, 1734, 1749
StyI CCWWGG 3 cut(s) 1239, 2027, 2401
TaaI ACNGT 7 cut(s) 311, 316, 544, 851, 1943, 1988, 2044
TaiI ACGT 2 cut(s) 790, 2375
TaqI TCGA 9 cut(s) 327, 478, 504, 1447, 1503, 1912, 1945, 2379, 2388
TaqII GACCGA 1 cut(s) 828
TatI WGTACW 3 cut(s) 829, 2202, 2325
TfiI GAWTC 5 cut(s) 244, 606, 1015, 1449, 1762
Tru1I TTAA 6 cut(s) 12, 71, 209, 389, 984, 2055
Tru9I TTAA 6 cut(s) 12, 71, 209, 389, 984, 2055
TscAI CASTG 4 cut(s) 547, 1167, 1387, 2179
TseFI GTSAC 5 cut(s) 798, 821, 1687, 2266, 2362
TseI GCWGC 2 cut(s) 1151, 1950
Tsp45I GTSAC 5 cut(s) 798, 821, 1687, 2266, 2362
TspDTI ATGAA 4 cut(s) 1247, 1526, 2148, 2246
TspGWI ACGGA 4 cut(s) 442, 1069, 1124, 2443
TspRI CASTG 4 cut(s) 547, 1167, 1387, 2179
Van91I CCANNNNNTGG 1 cut(s) 1558
VneI GTGCAC 1 cut(s) 1595
VpaK11BI GGWCC 1 cut(s) 1352
XagI CCTNNNNNAGG 2 cut(s) 1502, 1754
XapI RAATTY 4 cut(s) 533, 816, 2151, 2283
XceI RCATGY 3 cut(s) 364, 912, 1461
XcmI CCANNNNNNNNNTGG 2 cut(s) 111, 340
XhoI CTCGAG 1 cut(s) 1502
XmaJI CCTAGG 1 cut(s) 2027
XmiI GTMKAC 1 cut(s) 1051
XmnI GAANNNNTTC 1 cut(s) 2283
XspI CTAG 8 cut(s) 872, 960, 1361, 1386, 2028, 2034, 2271, 2459
Zsp2I ATGCAT 4 cut(s) 776, 910, 2077, 2422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.