Rmu_sc0003238.1_g000035

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003238.1
OG0001291
Physical Location & Seq
Reverse (-)
210693 .. 212524
1832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003238.1_g000035.1.cds

Sequence Viewer

Length: 1509 bp
atggagggtcggccattttttccattttatttgtccggccttgtgcttgctcccttcatcgttggttttgcaagcatcatggacaatgagcatggcggaagtgtggccaatcatttcaacaagctggacagcgaagacttggatcggggactgtctggttggattggaagcttcaagaagattgatgacggcgggaatggtccgatcggaatcatcagtgcccaaaccactaagatcattgctgggaatgttttctggtccaagaccttggaccagaaaacattcccagcaatgatcttagtggtttgggcactgatgattccgatcggaccattcctagcctacagttacaagaccaccaagcatttcgggcaatacaaatataggaacatacgaagcataggatccgacacagagcttcgcatccttgcatgcacccacgactcacgcaactcacccgccgtcatcaatcttcttgaagcttccaatccaaccagacagtccccgatccaagtcttcgctgtccagcttgttgaaatgattggccacacttccgccatgctcattgtccatgatgcttgcaaaaccaacgatgaagggagcaagcacaaggccggacaaatatcctcatccttgccctccaatccctttgaactctacgcaaaggaaagagagaatgtcagtgtccaggcactcaccatcgtctcggcctacgccacaatgcatgaggacatatgcaactttgcggaggacaagtgcattacccttgtgatggtcccgttccatcaacaatccaccctagacggaaacatggaggagtccaaccctgctctcagggcagtcaatagaaatgtcattgataacgcaccatgctcagtgggtgttcttgtggaccgtgggctcggtatatcccagatcttcaagtccaacatcagcagccatattagctccgatgacgtgagctaccaccgctttgctgtatttttcattggaggggcagatgaccgtgaggcattagcgtatgcaaaaaggatggctgggcattccagggttgacctaacagttgttcggttcatcttaaatacagaggcatgcttagatgatgctgactgcgacaacgaaattatagaggcaatggcaaacactgggaagcaaaaacagcttgatgacttgtacctggatggttttagggtccatacaatgcacaattcctccataaagttggtagaggaggtggtgaatagctgggaagaaactctggacgccataaacgacatggagggtgattatgatttgtacataatgggaagacgacatggaagcagcagcacgttggcagtgacatcagcattggacagcagcgactgtgatgagctaggtactctggcagaggcgttagtgtcttctagcttttttaaggttaacacatctattctcatagtgcagcaaggtgctgctgtagaggaaatagtcgaaatacaagatgagtcggataaccaggaaatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

502

Amino Acids

55.17

Weight (kDa)

4.84

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 96, 192, 459, 555, 746, 970
AclWI GGATC 4 cut(s) 150, 399, 412, 502
AcoI YGGCCR 3 cut(s) 11, 105, 544
AcyI GRCGYC 1 cut(s) 1263
AfaI GTAC 3 cut(s) 1175, 1298, 1381
AfiI CCNNNNNNNGG 1 cut(s) 772
AgsI TTSAA 6 cut(s) 118, 175, 479, 536, 653, 922
AjiI CACGTC 1 cut(s) 958
AjnI CCWGG 4 cut(s) 687, 1046, 1176, 1497
Alw26I GTCTC 1 cut(s) 709
AlwI GGATC 4 cut(s) 150, 399, 412, 502
AlwNI CAGNNNCTG 1 cut(s) 1365
AoxI GGCC 6 cut(s) 11, 37, 105, 544, 612, 708
ApeKI GCWGC 6 cut(s) 936, 1323, 1326, 1359, 1444, 1454
Asp700I GAANNNNTTC 2 cut(s) 251, 281
AspS9I GGNCC 7 cut(s) 200, 258, 271, 329, 775, 892, 1192
AsuHPI GGTGA 4 cut(s) 447, 688, 1249, 1295
AvaII GGWCC 7 cut(s) 200, 258, 271, 329, 775, 892, 1192
BaeGI GKGCMC 2 cut(s) 223, 313
BalI TGGCCA 2 cut(s) 107, 546
BamHI GGATCC 1 cut(s) 404
BanII GRGCYC 1 cut(s) 903
BbsI GAAGAC 4 cut(s) 141, 508, 1315, 1395
BbvI GCAGC 6 cut(s) 948, 1335, 1338, 1371, 1441, 1456
BccI CCATC 5 cut(s) 707, 766, 792, 1027, 1175
BceAI ACGGC 2 cut(s) 205, 446
BciT130I CCWGG 4 cut(s) 689, 1048, 1178, 1499
BcoDI GTCTC 1 cut(s) 709
BfaI CTAG 4 cut(s) 338, 800, 1376, 1407
BfmI CTRYAG 2 cut(s) 343, 1458
BglII AGATCT 1 cut(s) 915
BisI GCNGC 6 cut(s) 937, 1324, 1327, 1360, 1445, 1455
BlsI GCNGC 6 cut(s) 938, 1325, 1328, 1361, 1446, 1456
Bme1390I CCNGG 4 cut(s) 689, 1048, 1178, 1499
Bme18I GGWCC 7 cut(s) 200, 258, 271, 329, 775, 892, 1192
BmgBI CACGTC 1 cut(s) 958
BmgT120I GGNCC 7 cut(s) 200, 258, 271, 329, 775, 892, 1192
BmiI GGNNCC 3 cut(s) 406, 777, 1193
BmrFI CCNGG 4 cut(s) 689, 1048, 1178, 1499
BmrI ACTGGG 1 cut(s) 1155
BmsI GCATC 4 cut(s) 84, 432, 565, 1093
BmuI ACTGGG 1 cut(s) 1155
BpiI GAAGAC 4 cut(s) 141, 508, 1315, 1395
BsaBI GATNNNNATC 2 cut(s) 209, 323
BsaHI GRCGYC 1 cut(s) 1263
BsaJI CCNNGG 3 cut(s) 267, 895, 1047
BsaXI ACNNNNNCTCC 4 cut(s) 740, 770, 1196, 1226
Bsc4I CCNNNNNNNGG 1 cut(s) 772
Bse1I ACTGG 1 cut(s) 1150
Bse3DI GCAATG 3 cut(s) 237, 297, 1140
Bse8I GATNNNNATC 2 cut(s) 209, 323
BseBI CCWGG 4 cut(s) 689, 1048, 1178, 1499
BseDI CCNNGG 3 cut(s) 267, 895, 1047
BseGI GGATG 4 cut(s) 423, 629, 1038, 1186
BseJI GATNNNNATC 2 cut(s) 209, 323
BseLI CCNNNNNNNGG 1 cut(s) 772
BseMI GCAATG 3 cut(s) 237, 297, 1140
BseMII CTCAG 2 cut(s) 847, 888
BseNI ACTGG 1 cut(s) 1150
BseRI GAGGAG 2 cut(s) 830, 1244
BseSI GKGCMC 2 cut(s) 223, 313
BseXI GCAGC 6 cut(s) 948, 1335, 1338, 1371, 1441, 1456
BseYI CCCAGC 4 cut(s) 242, 286, 1037, 1245
BsgI GTGCAG 1 cut(s) 1463
Bsh1285I CGRYCG 2 cut(s) 207, 327
BshFI GGCC 6 cut(s) 13, 39, 107, 546, 614, 710
BsiEI CGRYCG 2 cut(s) 207, 327
BsiSI CCGG 2 cut(s) 36, 615
BslFI GGGAC 3 cut(s) 162, 487, 761
BslI CCNNNNNNNGG 1 cut(s) 772
BsmAI GTCTC 1 cut(s) 709
BsmBI CGTCTC 1 cut(s) 709
BsmFI GGGAC 3 cut(s) 162, 487, 761
BsmI GAATGC 1 cut(s) 1042
BsnI GGCC 6 cut(s) 13, 39, 107, 546, 614, 710
Bsp1286I GDGCHC 3 cut(s) 223, 313, 903
Bsp1407I TGTACA 1 cut(s) 1296
Bsp143I GATC 8 cut(s) 142, 204, 234, 294, 324, 404, 507, 915
BspACI CCGC 6 cut(s) 96, 192, 459, 555, 746, 970
BspANI GGCC 6 cut(s) 13, 39, 107, 546, 614, 710
BspCNI CTCAG 2 cut(s) 846, 887
BspLI GGNNCC 3 cut(s) 406, 777, 1193
BspPI GGATC 4 cut(s) 150, 399, 412, 502
BsrDI GCAATG 3 cut(s) 237, 297, 1140
BsrGI TGTACA 1 cut(s) 1296
BsrI ACTGG 1 cut(s) 1150
BssECI CCNNGG 3 cut(s) 267, 895, 1047
BssMI GATC 8 cut(s) 142, 204, 234, 294, 324, 404, 507, 915
BssNI GRCGYC 1 cut(s) 1263
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 4 cut(s) 689, 1048, 1178, 1499
Bst4CI ACNGT 7 cut(s) 153, 347, 501, 896, 1007, 1063, 1367
BstACI GRCGYC 1 cut(s) 1263
BstAUI TGTACA 1 cut(s) 1296
BstC8I GCNNGC 6 cut(s) 48, 73, 433, 580, 605, 1093
BstDEI CTNAG 5 cut(s) 231, 298, 833, 874, 1096
BstDSI CCRYGG 1 cut(s) 895
BstF5I GGATG 4 cut(s) 423, 629, 1038, 1186
BstKTI GATC 8 cut(s) 145, 207, 237, 297, 327, 407, 510, 918
BstMAI GTCTC 1 cut(s) 709
BstMBI GATC 8 cut(s) 142, 204, 234, 294, 324, 404, 507, 915
BstMCI CGRYCG 2 cut(s) 207, 327
BstMWI GCNNNNNNNGC 5 cut(s) 370, 836, 945, 969, 1159
BstNI CCWGG 4 cut(s) 689, 1048, 1178, 1499
BstNSI RCATGY 2 cut(s) 435, 1095
BstSCI CCNGG 4 cut(s) 687, 1046, 1176, 1497
BstSFI CTRYAG 2 cut(s) 343, 1458
BstSLI GKGCMC 2 cut(s) 223, 313
BstV1I GCAGC 6 cut(s) 948, 1335, 1338, 1371, 1441, 1456
BstV2I GAAGAC 4 cut(s) 141, 508, 1315, 1395
BstX2I RGATCY 2 cut(s) 404, 915
BstXI CCANNNNNNTGG 2 cut(s) 268, 1222
BstYI RGATCY 2 cut(s) 404, 915
BsuRI GGCC 6 cut(s) 13, 39, 107, 546, 614, 710
BtgI CCRYGG 1 cut(s) 895
BtrI CACGTC 1 cut(s) 958
BtsCI GGATG 4 cut(s) 423, 629, 1038, 1186
BtsI GCAGTG 1 cut(s) 1344
BtsIMutI CAGTG 6 cut(s) 223, 311, 688, 882, 1143, 1344
Cac8I GCNNGC 6 cut(s) 48, 73, 433, 580, 605, 1093
CaiI CAGNNNCTG 1 cut(s) 1365
Cfr13I GGNCC 7 cut(s) 200, 258, 271, 329, 775, 892, 1192
CseI GACGC 1 cut(s) 1271
Csp6I GTAC 3 cut(s) 1174, 1297, 1380
CviQI GTAC 3 cut(s) 1174, 1297, 1380
DdeI CTNAG 5 cut(s) 231, 298, 833, 874, 1096
DpnI GATC 8 cut(s) 144, 206, 236, 296, 326, 406, 509, 917
DpnII GATC 8 cut(s) 142, 204, 234, 294, 324, 404, 507, 915
EaeI YGGCCR 3 cut(s) 11, 105, 544
EciI GGCGGA 2 cut(s) 111, 544
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 903
Eco47I GGWCC 7 cut(s) 200, 258, 271, 329, 775, 892, 1192
EcoRII CCWGG 4 cut(s) 687, 1046, 1176, 1497
EcoT14I CCWWGG 1 cut(s) 267
EcoT22I ATGCAT 1 cut(s) 726
EcoT38I GRGCYC 1 cut(s) 903
ErhI CCWWGG 1 cut(s) 267
Esp3I CGTCTC 1 cut(s) 709
FaqI GGGAC 3 cut(s) 162, 487, 761
FauI CCCGC 2 cut(s) 185, 466
FauNDI CATATG 1 cut(s) 734
Fnu4HI GCNGC 6 cut(s) 937, 1324, 1327, 1360, 1445, 1455
FokI GGATG 4 cut(s) 410, 616, 1045, 1193
FriOI GRGCYC 1 cut(s) 903
Fsp4HI GCNGC 6 cut(s) 937, 1324, 1327, 1360, 1445, 1455
FspBI CTAG 4 cut(s) 338, 800, 1376, 1407
GluI GCNGC 6 cut(s) 937, 1324, 1327, 1360, 1445, 1455
GsaI CCCAGC 4 cut(s) 246, 290, 1041, 1249
HaeIII GGCC 6 cut(s) 13, 39, 107, 546, 614, 710
HapII CCGG 2 cut(s) 36, 615
HgaI GACGC 1 cut(s) 1271
Hin1I GRCGYC 1 cut(s) 1263
HincII GTYRAC 2 cut(s) 1054, 1423
HindII GTYRAC 2 cut(s) 1054, 1423
HindIII AAGCTT 2 cut(s) 169, 480
HinfI GANTC 5 cut(s) 210, 319, 443, 818, 1487
HpaI GTTAAC 1 cut(s) 1423
HpaII CCGG 2 cut(s) 36, 615
HphI GGTGA 4 cut(s) 447, 688, 1249, 1295
Hpy166II GTNNAC 3 cut(s) 892, 1054, 1423
Hpy188I TCNGA 7 cut(s) 204, 209, 324, 329, 409, 952, 1492
Hpy188III TCNNGA 3 cut(s) 175, 476, 1259
Hpy8I GTNNAC 3 cut(s) 892, 1054, 1423
HpyAV CCTTC 2 cut(s) 64, 590
HpyCH4III ACNGT 7 cut(s) 153, 347, 501, 896, 1007, 1063, 1367
HpyCH4IV ACGT 2 cut(s) 957, 1331
HpyF10VI GCNNNNNNNGC 5 cut(s) 370, 836, 945, 969, 1159
HpyF3I CTNAG 5 cut(s) 231, 298, 833, 874, 1096
HpySE526I ACGT 2 cut(s) 957, 1331
Hsp92I GRCGYC 1 cut(s) 1263
KspAI GTTAAC 1 cut(s) 1423
Kzo9I GATC 8 cut(s) 142, 204, 234, 294, 324, 404, 507, 915
LmnI GCTCC 3 cut(s) 55, 600, 953
Lsp1109I GCAGC 6 cut(s) 948, 1335, 1338, 1371, 1441, 1456
LweI GCATC 4 cut(s) 84, 432, 565, 1093
MaeI CTAG 4 cut(s) 338, 800, 1376, 1407
MaeII ACGT 2 cut(s) 957, 1331
MaeIII GTNAC 2 cut(s) 347, 1339
MalI GATC 8 cut(s) 144, 206, 236, 296, 326, 406, 509, 917
MboI GATC 8 cut(s) 142, 204, 234, 294, 324, 404, 507, 915
MboII GAAGA 8 cut(s) 146, 190, 464, 508, 910, 1262, 1320, 1395
MflI RGATCY 2 cut(s) 404, 915
MhlI GDGCHC 3 cut(s) 223, 313, 903
MlsI TGGCCA 2 cut(s) 107, 546
MluCI AATT 2 cut(s) 1122, 1207
MluNI TGGCCA 2 cut(s) 107, 546
MlyI GAGTC 3 cut(s) 437, 827, 1496
MmeI TCCRAC 6 cut(s) 140, 432, 515, 846, 951, 1470
Mox20I TGGCCA 2 cut(s) 107, 546
Mph1103I ATGCAT 1 cut(s) 726
MroXI GAANNNNTTC 2 cut(s) 251, 281
MscI TGGCCA 2 cut(s) 107, 546
MseI TTAA 3 cut(s) 1079, 1416, 1422
Msp20I TGGCCA 2 cut(s) 107, 546
MspI CCGG 2 cut(s) 36, 615
MspR9I CCNGG 4 cut(s) 689, 1048, 1178, 1499
Mva1269I GAATGC 1 cut(s) 1042
MvaI CCWGG 4 cut(s) 689, 1048, 1178, 1499
MwoI GCNNNNNNNGC 5 cut(s) 370, 836, 945, 969, 1159
NdeI CATATG 1 cut(s) 734
NdeII GATC 8 cut(s) 142, 204, 234, 294, 324, 404, 507, 915
NlaIV GGNNCC 3 cut(s) 406, 777, 1193
NmeAIII GCCGAG 1 cut(s) 686
NmuCI GTSAC 1 cut(s) 1339
NsiI ATGCAT 1 cut(s) 726
NspI RCATGY 2 cut(s) 435, 1095
PaeI GCATGC 2 cut(s) 435, 1095
PcsI WCGNNNNNNNCGW 1 cut(s) 1269
PctI GAATGC 1 cut(s) 1042
PdmI GAANNNNTTC 2 cut(s) 251, 281
PfeI GAWTC 2 cut(s) 210, 319
PkrI GCNGC 6 cut(s) 938, 1325, 1328, 1361, 1446, 1456
Ple19I CGATCG 2 cut(s) 207, 327
PleI GAGTC 3 cut(s) 437, 826, 1495
PpsI GAGTC 3 cut(s) 437, 826, 1495
Psp6I CCWGG 4 cut(s) 687, 1046, 1176, 1497
PspFI CCCAGC 4 cut(s) 242, 286, 1037, 1245
PspGI CCWGG 4 cut(s) 687, 1046, 1176, 1497
PspN4I GGNNCC 3 cut(s) 406, 777, 1193
PspPI GGNCC 7 cut(s) 200, 258, 271, 329, 775, 892, 1192
PstNI CAGNNNCTG 1 cut(s) 1365
PsuI RGATCY 2 cut(s) 404, 915
PvuI CGATCG 2 cut(s) 207, 327
RsaI GTAC 3 cut(s) 1175, 1298, 1381
RsaNI GTAC 3 cut(s) 1174, 1297, 1380
SaqAI TTAA 3 cut(s) 1079, 1416, 1422
SatI GCNGC 6 cut(s) 937, 1324, 1327, 1360, 1445, 1455
Sau3AI GATC 8 cut(s) 142, 204, 234, 294, 324, 404, 507, 915
Sau96I GGNCC 7 cut(s) 200, 258, 271, 329, 775, 892, 1192
SchI GAGTC 3 cut(s) 437, 827, 1496
ScrFI CCNGG 4 cut(s) 689, 1048, 1178, 1499
SduI GDGCHC 3 cut(s) 223, 313, 903
SfaNI GCATC 4 cut(s) 84, 432, 565, 1093
SfcI CTRYAG 2 cut(s) 343, 1458
SinI GGWCC 7 cut(s) 200, 258, 271, 329, 775, 892, 1192
SphI GCATGC 2 cut(s) 435, 1095
Sse9I AATT 2 cut(s) 1122, 1207
SsiI CCGC 6 cut(s) 96, 192, 459, 555, 746, 970
SspMI CTAG 4 cut(s) 338, 800, 1376, 1407
StyD4I CCNGG 4 cut(s) 687, 1046, 1176, 1497
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 7 cut(s) 153, 347, 501, 896, 1007, 1063, 1367
TaiI ACGT 2 cut(s) 960, 1334
TaqI TCGA 1 cut(s) 1473
TasI AATT 2 cut(s) 1122, 1207
TatI WGTACW 1 cut(s) 1296
TfiI GAWTC 2 cut(s) 210, 319
Tru1I TTAA 3 cut(s) 1079, 1416, 1422
Tru9I TTAA 3 cut(s) 1079, 1416, 1422
TscAI CASTG 6 cut(s) 223, 318, 688, 882, 1150, 1344
TseFI GTSAC 1 cut(s) 1339
TseI GCWGC 6 cut(s) 936, 1323, 1326, 1359, 1444, 1454
Tsp45I GTSAC 1 cut(s) 1339
TspDTI ATGAA 4 cut(s) 46, 609, 976, 1063
TspGWI ACGGA 1 cut(s) 819
TspRI CASTG 6 cut(s) 223, 318, 688, 882, 1150, 1344
VpaK11BI GGWCC 7 cut(s) 200, 258, 271, 329, 775, 892, 1192
XceI RCATGY 2 cut(s) 435, 1095
XcmI CCANNNNNNNNNTGG 1 cut(s) 1273
XmnI GAANNNNTTC 2 cut(s) 251, 281
XspI CTAG 4 cut(s) 338, 800, 1376, 1407
Zsp2I ATGCAT 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.