Rw5G019460

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001291
Physical Location & Seq
Reverse (-)
25327081 .. 25328298
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G019460.1

Sequence Viewer

Length: 1218 bp
ATGATCTTAGTGGTTTGGGCACTGATGATTCCGATCGGACCATTCCTAGCCTACAGTTACAAGACCACCAAGCATTTCGGGCAATACAAATATAGGAACATACGAAGCATAGGATCCGACACAGAGCTTCGCATCCTTGCATGCACCCACGACTCACGCAACTCACCCGCCGTCATCAATCTTCTTGAAGCTTCCAATCCAACCAGACAGTCCCCGATCCAAGTCTTCGCTGTCCAGCTTGTTGAAATGATTGGCCACACTTCCGCCATGCTCATTGTCCATGATGCTTGCAAAACCAACGATGAAGGGAGCAAGCACAAGGCCGGACAAATATCCTCATCCTTGCCCTCCAATCCCTTTGAACTCTACGCAAAGGAAAGAGAGAATGTCAGTGTCCAGGCACTCACCATCGTCTCGGCCTACGCCACAATGCATGAGGACATATGCAACTTTGCGGAGGACAAGTGCATTACCCTTGTGATGGTCCCGTTCCATCAACAATCCACCCTAGACGGAAACATGGAGGAGTCCAACCCTGCTCTCAGGGCAGTCAATAGAAATGTCATTGATAACGCACCATGCTCAGTGGGTGTTCTTGTGGACCGTGGGCTCGGTATATCCCAGATCTTCAAGTCCAACATCAGCAGCCATATTAGCTCCGATGACGTGAGCTACCACCGCTTTGCTGTATTTTTCATTGGAGGGGCAGATGACCGTGAGGCATTAGCGTATGCAAAAAGGATGGCTGGGCATTCCAGGGTTGACCTAACAGTTGTTCGGTTCATCTTAAATACAGAGGCATGCTTAGATGATGCTGACTGCGACAACGAAATTATAGAGGCAATGGCAAACACTGGGAAGCAAAAACAGCTTGATGACTTGTACCTGGATGGTTTTAGGGTCCATACAATGCACAATTCCTCCATAAAGTTGGTAGAGGAGGTGGTGAATAGCTGGGAAGAAACTCTGGACGCCATAAACGACATGGAGGGTGATTATGATTTGTACATAATGGGAAGACGACATGGAAGCAGCAGCACGTTGGCAGTGACATCAGCATTGGACAGCAGCGACTGTGATGAGCTAGGTACTCTGGCAGAGGCGTTAGTGTCTTCTAGCTTTTTTAAGGTTAACACATCTATTCTCATAGTGCAGCAAGGTGCTGCTGTAGAGGAAATAGTCGAAATACAAGATGAGTCGGATAACCAGGAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

405

Amino Acids

44.71

Weight (kDa)

4.76

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHX17_2nd PF23256 75 - 206 2.5e-17 Plant cation/H(+) antiporter domain
CHX17_C PF23259 229 - 386 3.9e-12 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 168, 264, 455, 679
AclWI GGATC 3 cut(s) 108, 121, 211
AcoI YGGCCR 1 cut(s) 253
AcyI GRCGYC 1 cut(s) 972
AfaI GTAC 3 cut(s) 884, 1007, 1090
AfiI CCNNNNNNNGG 1 cut(s) 481
AgsI TTSAA 4 cut(s) 188, 245, 362, 631
AjiI CACGTC 1 cut(s) 667
AjnI CCWGG 4 cut(s) 396, 755, 885, 1206
AluBI AGCT 9 cut(s) 127, 191, 238, 657, 672, 871, 954, 1084, 1119
AluI AGCT 9 cut(s) 127, 191, 238, 657, 672, 871, 954, 1084, 1119
Alw26I GTCTC 1 cut(s) 418
AlwI GGATC 3 cut(s) 108, 121, 211
AlwNI CAGNNNCTG 1 cut(s) 1074
AoxI GGCC 3 cut(s) 253, 321, 417
ApeKI GCWGC 6 cut(s) 645, 1032, 1035, 1068, 1153, 1163
AspS9I GGNCC 4 cut(s) 38, 484, 601, 901
AsuHPI GGTGA 4 cut(s) 156, 397, 958, 1004
AvaII GGWCC 4 cut(s) 38, 484, 601, 901
BaeGI GKGCMC 1 cut(s) 22
BalI TGGCCA 1 cut(s) 255
BamHI GGATCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 612
BbsI GAAGAC 3 cut(s) 217, 1024, 1104
BbvI GCAGC 6 cut(s) 657, 1044, 1047, 1080, 1150, 1165
BccI CCATC 5 cut(s) 416, 475, 501, 736, 884
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 4 cut(s) 398, 757, 887, 1208
BcoDI GTCTC 1 cut(s) 418
BfaI CTAG 4 cut(s) 47, 509, 1085, 1116
BfmI CTRYAG 2 cut(s) 52, 1167
BglII AGATCT 1 cut(s) 624
BisI GCNGC 6 cut(s) 646, 1033, 1036, 1069, 1154, 1164
BlsI GCNGC 6 cut(s) 647, 1034, 1037, 1070, 1155, 1165
Bme1390I CCNGG 4 cut(s) 398, 757, 887, 1208
Bme18I GGWCC 4 cut(s) 38, 484, 601, 901
BmgBI CACGTC 1 cut(s) 667
BmgT120I GGNCC 4 cut(s) 38, 484, 601, 901
BmiI GGNNCC 3 cut(s) 115, 486, 902
BmrFI CCNGG 4 cut(s) 398, 757, 887, 1208
BmrI ACTGGG 1 cut(s) 864
BmsI GCATC 3 cut(s) 141, 274, 802
BmuI ACTGGG 1 cut(s) 864
BpiI GAAGAC 3 cut(s) 217, 1024, 1104
BsaBI GATNNNNATC 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 972
BsaJI CCNNGG 2 cut(s) 604, 756
BsaXI ACNNNNNCTCC 4 cut(s) 449, 479, 905, 935
Bsc4I CCNNNNNNNGG 1 cut(s) 481
Bse1I ACTGG 1 cut(s) 859
Bse3DI GCAATG 1 cut(s) 849
Bse8I GATNNNNATC 1 cut(s) 32
BseBI CCWGG 4 cut(s) 398, 757, 887, 1208
BseDI CCNNGG 2 cut(s) 604, 756
BseGI GGATG 4 cut(s) 132, 338, 747, 895
BseJI GATNNNNATC 1 cut(s) 32
BseLI CCNNNNNNNGG 1 cut(s) 481
BseMI GCAATG 1 cut(s) 849
BseMII CTCAG 2 cut(s) 556, 597
BseNI ACTGG 1 cut(s) 859
BseRI GAGGAG 2 cut(s) 539, 953
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 6 cut(s) 657, 1044, 1047, 1080, 1150, 1165
BseYI CCCAGC 2 cut(s) 746, 954
BsgI GTGCAG 1 cut(s) 1172
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 3 cut(s) 255, 323, 419
BsiEI CGRYCG 1 cut(s) 36
BsiSI CCGG 1 cut(s) 324
BslFI GGGAC 2 cut(s) 196, 470
BslI CCNNNNNNNGG 1 cut(s) 481
BsmAI GTCTC 1 cut(s) 418
BsmBI CGTCTC 1 cut(s) 418
BsmFI GGGAC 2 cut(s) 196, 470
BsmI GAATGC 1 cut(s) 751
BsnI GGCC 3 cut(s) 255, 323, 419
Bsp1286I GDGCHC 2 cut(s) 22, 612
Bsp1407I TGTACA 1 cut(s) 1005
Bsp143I GATC 5 cut(s) 3, 33, 113, 216, 624
BspACI CCGC 4 cut(s) 168, 264, 455, 679
BspANI GGCC 3 cut(s) 255, 323, 419
BspCNI CTCAG 2 cut(s) 555, 596
BspLI GGNNCC 3 cut(s) 115, 486, 902
BspPI GGATC 3 cut(s) 108, 121, 211
BsrDI GCAATG 1 cut(s) 849
BsrGI TGTACA 1 cut(s) 1005
BsrI ACTGG 1 cut(s) 859
BssECI CCNNGG 2 cut(s) 604, 756
BssMI GATC 5 cut(s) 3, 33, 113, 216, 624
BssNI GRCGYC 1 cut(s) 972
Bst2UI CCWGG 4 cut(s) 398, 757, 887, 1208
Bst4CI ACNGT 6 cut(s) 56, 210, 605, 716, 772, 1076
BstACI GRCGYC 1 cut(s) 972
BstAUI TGTACA 1 cut(s) 1005
BstC8I GCNNGC 4 cut(s) 142, 289, 314, 802
BstDEI CTNAG 4 cut(s) 7, 542, 583, 805
BstDSI CCRYGG 1 cut(s) 604
BstF5I GGATG 4 cut(s) 132, 338, 747, 895
BstKTI GATC 5 cut(s) 6, 36, 116, 219, 627
BstMAI GTCTC 1 cut(s) 418
BstMBI GATC 5 cut(s) 3, 33, 113, 216, 624
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 5 cut(s) 79, 545, 654, 678, 868
BstNI CCWGG 4 cut(s) 398, 757, 887, 1208
BstNSI RCATGY 2 cut(s) 144, 804
BstSCI CCNGG 4 cut(s) 396, 755, 885, 1206
BstSFI CTRYAG 2 cut(s) 52, 1167
BstSLI GKGCMC 1 cut(s) 22
BstV1I GCAGC 6 cut(s) 657, 1044, 1047, 1080, 1150, 1165
BstV2I GAAGAC 3 cut(s) 217, 1024, 1104
BstX2I RGATCY 2 cut(s) 113, 624
BstXI CCANNNNNNTGG 1 cut(s) 931
BstYI RGATCY 2 cut(s) 113, 624
BsuRI GGCC 3 cut(s) 255, 323, 419
BtgI CCRYGG 1 cut(s) 604
BtrI CACGTC 1 cut(s) 667
BtsCI GGATG 4 cut(s) 132, 338, 747, 895
BtsI GCAGTG 1 cut(s) 1053
BtsIMutI CAGTG 5 cut(s) 20, 397, 591, 852, 1053
Cac8I GCNNGC 4 cut(s) 142, 289, 314, 802
CaiI CAGNNNCTG 1 cut(s) 1074
Cfr13I GGNCC 4 cut(s) 38, 484, 601, 901
CseI GACGC 1 cut(s) 980
Csp6I GTAC 3 cut(s) 883, 1006, 1089
CviAII CATG 9 cut(s) 141, 268, 281, 434, 520, 579, 801, 985, 1025
CviQI GTAC 3 cut(s) 883, 1006, 1089
DdeI CTNAG 4 cut(s) 7, 542, 583, 805
DpnI GATC 5 cut(s) 5, 35, 115, 218, 626
DpnII GATC 5 cut(s) 3, 33, 113, 216, 624
EaeI YGGCCR 1 cut(s) 253
EciI GGCGGA 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 612
Eco47I GGWCC 4 cut(s) 38, 484, 601, 901
EcoRII CCWGG 4 cut(s) 396, 755, 885, 1206
EcoT22I ATGCAT 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 612
Esp3I CGTCTC 1 cut(s) 418
FaeI CATG 9 cut(s) 144, 271, 284, 437, 523, 582, 804, 988, 1028
FaqI GGGAC 2 cut(s) 196, 470
FatI CATG 9 cut(s) 140, 267, 280, 433, 519, 578, 800, 984, 1024
FauI CCCGC 1 cut(s) 175
FauNDI CATATG 1 cut(s) 443
Fnu4HI GCNGC 6 cut(s) 646, 1033, 1036, 1069, 1154, 1164
FokI GGATG 4 cut(s) 119, 325, 754, 902
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 6 cut(s) 646, 1033, 1036, 1069, 1154, 1164
FspBI CTAG 4 cut(s) 47, 509, 1085, 1116
GluI GCNGC 6 cut(s) 646, 1033, 1036, 1069, 1154, 1164
GsaI CCCAGC 2 cut(s) 750, 958
HaeIII GGCC 3 cut(s) 255, 323, 419
HapII CCGG 1 cut(s) 324
HgaI GACGC 1 cut(s) 980
Hin1I GRCGYC 1 cut(s) 972
Hin1II CATG 9 cut(s) 144, 271, 284, 437, 523, 582, 804, 988, 1028
HincII GTYRAC 2 cut(s) 763, 1132
HindII GTYRAC 2 cut(s) 763, 1132
HindIII AAGCTT 1 cut(s) 189
HinfI GANTC 4 cut(s) 28, 152, 527, 1196
HpaI GTTAAC 1 cut(s) 1132
HpaII CCGG 1 cut(s) 324
HphI GGTGA 4 cut(s) 156, 397, 958, 1004
Hpy166II GTNNAC 3 cut(s) 601, 763, 1132
Hpy188I TCNGA 5 cut(s) 33, 38, 118, 661, 1201
Hpy188III TCNNGA 2 cut(s) 185, 968
Hpy8I GTNNAC 3 cut(s) 601, 763, 1132
HpyAV CCTTC 1 cut(s) 299
HpyCH4III ACNGT 6 cut(s) 56, 210, 605, 716, 772, 1076
HpyCH4IV ACGT 2 cut(s) 666, 1040
HpyCH4V TGCA 9 cut(s) 140, 144, 291, 433, 447, 468, 734, 913, 1153
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 545, 654, 678, 868
HpyF3I CTNAG 4 cut(s) 7, 542, 583, 805
HpySE526I ACGT 2 cut(s) 666, 1040
Hsp92I GRCGYC 1 cut(s) 972
Hsp92II CATG 9 cut(s) 144, 271, 284, 437, 523, 582, 804, 988, 1028
KspAI GTTAAC 1 cut(s) 1132
Kzo9I GATC 5 cut(s) 3, 33, 113, 216, 624
LmnI GCTCC 2 cut(s) 309, 662
Lsp1109I GCAGC 6 cut(s) 657, 1044, 1047, 1080, 1150, 1165
LweI GCATC 3 cut(s) 141, 274, 802
MaeI CTAG 4 cut(s) 47, 509, 1085, 1116
MaeII ACGT 2 cut(s) 666, 1040
MaeIII GTNAC 2 cut(s) 56, 1048
MalI GATC 5 cut(s) 5, 35, 115, 218, 626
MboI GATC 5 cut(s) 3, 33, 113, 216, 624
MboII GAAGA 6 cut(s) 173, 217, 619, 971, 1029, 1104
MflI RGATCY 2 cut(s) 113, 624
MhlI GDGCHC 2 cut(s) 22, 612
MlsI TGGCCA 1 cut(s) 255
MluCI AATT 2 cut(s) 831, 916
MluNI TGGCCA 1 cut(s) 255
MlyI GAGTC 3 cut(s) 146, 536, 1205
MmeI TCCRAC 5 cut(s) 141, 224, 555, 660, 1179
Mox20I TGGCCA 1 cut(s) 255
Mph1103I ATGCAT 1 cut(s) 435
MscI TGGCCA 1 cut(s) 255
MseI TTAA 3 cut(s) 788, 1125, 1131
Msp20I TGGCCA 1 cut(s) 255
MspI CCGG 1 cut(s) 324
MspR9I CCNGG 4 cut(s) 398, 757, 887, 1208
Mva1269I GAATGC 1 cut(s) 751
MvaI CCWGG 4 cut(s) 398, 757, 887, 1208
MwoI GCNNNNNNNGC 5 cut(s) 79, 545, 654, 678, 868
NdeI CATATG 1 cut(s) 443
NdeII GATC 5 cut(s) 3, 33, 113, 216, 624
NlaIII CATG 9 cut(s) 144, 271, 284, 437, 523, 582, 804, 988, 1028
NlaIV GGNNCC 3 cut(s) 115, 486, 902
NmeAIII GCCGAG 1 cut(s) 395
NmuCI GTSAC 1 cut(s) 1048
NsiI ATGCAT 1 cut(s) 435
NspI RCATGY 2 cut(s) 144, 804
PaeI GCATGC 2 cut(s) 144, 804
PcsI WCGNNNNNNNCGW 1 cut(s) 978
PctI GAATGC 1 cut(s) 751
PfeI GAWTC 1 cut(s) 28
PkrI GCNGC 6 cut(s) 647, 1034, 1037, 1070, 1155, 1165
Ple19I CGATCG 1 cut(s) 36
PleI GAGTC 3 cut(s) 146, 535, 1204
PpsI GAGTC 3 cut(s) 146, 535, 1204
Psp6I CCWGG 4 cut(s) 396, 755, 885, 1206
PspFI CCCAGC 2 cut(s) 746, 954
PspGI CCWGG 4 cut(s) 396, 755, 885, 1206
PspN4I GGNNCC 3 cut(s) 115, 486, 902
PspPI GGNCC 4 cut(s) 38, 484, 601, 901
PstNI CAGNNNCTG 1 cut(s) 1074
PsuI RGATCY 2 cut(s) 113, 624
PvuI CGATCG 1 cut(s) 36
RsaI GTAC 3 cut(s) 884, 1007, 1090
RsaNI GTAC 3 cut(s) 883, 1006, 1089
SaqAI TTAA 3 cut(s) 788, 1125, 1131
SatI GCNGC 6 cut(s) 646, 1033, 1036, 1069, 1154, 1164
Sau3AI GATC 5 cut(s) 3, 33, 113, 216, 624
Sau96I GGNCC 4 cut(s) 38, 484, 601, 901
SchI GAGTC 3 cut(s) 146, 536, 1205
ScrFI CCNGG 4 cut(s) 398, 757, 887, 1208
SduI GDGCHC 2 cut(s) 22, 612
SfaNI GCATC 3 cut(s) 141, 274, 802
SfcI CTRYAG 2 cut(s) 52, 1167
SinI GGWCC 4 cut(s) 38, 484, 601, 901
SphI GCATGC 2 cut(s) 144, 804
Sse9I AATT 2 cut(s) 831, 916
SsiI CCGC 4 cut(s) 168, 264, 455, 679
SspMI CTAG 4 cut(s) 47, 509, 1085, 1116
StyD4I CCNGG 4 cut(s) 396, 755, 885, 1206
TaaI ACNGT 6 cut(s) 56, 210, 605, 716, 772, 1076
TaiI ACGT 2 cut(s) 669, 1043
TaqI TCGA 1 cut(s) 1182
TasI AATT 2 cut(s) 831, 916
TatI WGTACW 1 cut(s) 1005
TfiI GAWTC 1 cut(s) 28
Tru1I TTAA 3 cut(s) 788, 1125, 1131
Tru9I TTAA 3 cut(s) 788, 1125, 1131
TscAI CASTG 5 cut(s) 27, 397, 591, 859, 1053
TseFI GTSAC 1 cut(s) 1048
TseI GCWGC 6 cut(s) 645, 1032, 1035, 1068, 1153, 1163
Tsp45I GTSAC 1 cut(s) 1048
TspDTI ATGAA 3 cut(s) 318, 685, 772
TspGWI ACGGA 1 cut(s) 528
TspRI CASTG 5 cut(s) 27, 397, 591, 859, 1053
VpaK11BI GGWCC 4 cut(s) 38, 484, 601, 901
XceI RCATGY 2 cut(s) 144, 804
XcmI CCANNNNNNNNNTGG 1 cut(s) 982
XspI CTAG 4 cut(s) 47, 509, 1085, 1116
Zsp2I ATGCAT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.