MD02G1284900.v1.1

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001291
Physical Location & Seq
Reverse (-)
34137867 .. 34138406
540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1284900.v1.1.491

Sequence Viewer

Length: 540 bp
ATGCTCTTCTTTGGAGGGGTAGATGACCGTGAGGCATTAGCGTATGCGGGTCGCATGGCTGGACATCCTAGGACTAGCCTAACAGTCATATCGTTTAACATCATGAGTAAAAATGCATCAAACTTGTGCTCTGAGGATGATCATGGTGATGATGATGACGAAGACGACGACGATGAAGATGGTACTGGTGGAATTCTGAAGGCAATGGCAAGCACTGGGAGGGAAAAAAAACTGGATGAGTTGTACTTGGATGAGTTTAGGCTGATGTCAATGAATGATGCCTCCATAAAACTTTTAGAGAAATCGGTGACTAGGTGGGAACAAATTCTCTCACTAATAAGCTCAATGGAAGGTGAGTATGATATGTACATAGTAGGAAGAAGGCATGGAAATATATCAGAGGTCACCACGACGTTACTCGATGGCATCGACTCCAATGACCTTGGATTTCTTGGAGATGCATTGGTATCTTCAAACCTTACAGCAAGTACATCCGTTCTAGTTGTGCAACAAGGTCGCCTTTTGGATTATTATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

180

Amino Acids

19.71

Weight (kDa)

4.25

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHX17_C PF23259 2 - 31 5e-07 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 47
AcsI RAATTY 2 cut(s) 192, 324
AcuI CTGAAG 1 cut(s) 218
AfaI GTAC 4 cut(s) 184, 245, 368, 490
AgsI TTSAA 1 cut(s) 474
AluBI AGCT 1 cut(s) 342
AluI AGCT 1 cut(s) 342
Alw21I GWGCWC 1 cut(s) 131
ApoI RAATTY 2 cut(s) 192, 324
Asp700I GAANNNNTTC 1 cut(s) 324
AspA2I CCTAGG 1 cut(s) 68
AsuHPI GGTGA 4 cut(s) 158, 319, 365, 397
AvrII CCTAGG 1 cut(s) 68
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 131
BccI CCATC 2 cut(s) 173, 416
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BclI TGATCA 1 cut(s) 139
BfaI CTAG 4 cut(s) 69, 75, 312, 500
BlnI CCTAGG 1 cut(s) 68
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 4 cut(s) 125, 268, 435, 448
BmuI ACTGGG 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 68, 442
Bse1I ACTGG 3 cut(s) 190, 220, 237
Bse3DI GCAATG 1 cut(s) 210
BseDI CCNNGG 2 cut(s) 68, 442
BseGI GGATG 5 cut(s) 64, 142, 241, 256, 491
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 3 cut(s) 190, 220, 237
BsiHKAI GWGCWC 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 131
Bsp1407I TGTACA 1 cut(s) 366
Bsp143I GATC 1 cut(s) 139
BspACI CCGC 1 cut(s) 47
BspCNI CTCAG 1 cut(s) 124
BspHI TCATGA 1 cut(s) 102
BspQI GCTCTTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 210
BsrGI TGTACA 1 cut(s) 366
BsrI ACTGG 3 cut(s) 190, 220, 237
BssECI CCNNGG 2 cut(s) 68, 442
BssMI GATC 1 cut(s) 139
BssT1I CCWWGG 2 cut(s) 68, 442
Bst4CI ACNGT 2 cut(s) 29, 85
Bst6I CTCTTC 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 1 cut(s) 132
BstEII GGTNACC 1 cut(s) 403
BstF5I GGATG 5 cut(s) 64, 142, 241, 256, 491
BstKTI GATC 1 cut(s) 142
BstMBI GATC 1 cut(s) 139
BstPI GGTNACC 1 cut(s) 403
BstV2I GAAGAC 1 cut(s) 168
BtsCI GGATG 5 cut(s) 64, 142, 241, 256, 491
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 1 cut(s) 211
CciI TCATGA 1 cut(s) 102
Csp6I GTAC 4 cut(s) 183, 244, 367, 489
CviAII CATG 4 cut(s) 55, 103, 143, 386
CviJI RGCY 4 cut(s) 59, 78, 262, 342
CviKI_1 RGCY 4 cut(s) 59, 78, 262, 342
CviQI GTAC 4 cut(s) 183, 244, 367, 489
DdeI CTNAG 1 cut(s) 132
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Eco130I CCWWGG 2 cut(s) 68, 442
Eco57I CTGAAG 1 cut(s) 218
Eco91I GGTNACC 1 cut(s) 403
EcoO65I GGTNACC 1 cut(s) 403
EcoRI GAATTC 1 cut(s) 192
EcoT14I CCWWGG 2 cut(s) 68, 442
EcoT22I ATGCAT 2 cut(s) 118, 463
ErhI CCWWGG 2 cut(s) 68, 442
FaeI CATG 4 cut(s) 58, 106, 146, 389
FalI AAGNNNNNCTT 2 cut(s) 504, 536
FatI CATG 4 cut(s) 54, 102, 142, 385
FauI CCCGC 1 cut(s) 40
FbaI TGATCA 1 cut(s) 139
FokI GGATG 5 cut(s) 51, 149, 248, 263, 478
FspBI CTAG 4 cut(s) 69, 75, 312, 500
Hin1II CATG 4 cut(s) 58, 106, 146, 389
HinfI GANTC 1 cut(s) 431
HphI GGTGA 4 cut(s) 158, 319, 365, 397
Hpy188I TCNGA 3 cut(s) 133, 198, 400
Hpy188III TCNNGA 2 cut(s) 103, 537
Hpy99I CGWCG 3 cut(s) 170, 173, 415
HpyAV CCTTC 3 cut(s) 193, 344, 375
HpyCH4III ACNGT 2 cut(s) 29, 85
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 3 cut(s) 116, 461, 508
HpyF3I CTNAG 1 cut(s) 132
HpySE526I ACGT 1 cut(s) 413
Hsp92II CATG 4 cut(s) 58, 106, 146, 389
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 1 cut(s) 139
LguI GCTCTTC 1 cut(s) 11
LpnPI CCDG 4 cut(s) 45, 171, 201, 218
LweI GCATC 4 cut(s) 125, 268, 435, 448
MaeI CTAG 4 cut(s) 69, 75, 312, 500
MaeII ACGT 1 cut(s) 413
MaeIII GTNAC 3 cut(s) 307, 403, 414
MalI GATC 1 cut(s) 141
MboI GATC 1 cut(s) 139
MboII GAAGA 4 cut(s) 173, 188, 390, 462
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 2 cut(s) 192, 324
MlyI GAGTC 1 cut(s) 425
MnlI CCTC 6 cut(s) 8, 25, 127, 213, 292, 394
Mph1103I ATGCAT 2 cut(s) 118, 463
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 147
NdeII GATC 1 cut(s) 139
NlaIII CATG 4 cut(s) 58, 106, 146, 389
NmuCI GTSAC 2 cut(s) 307, 403
NsiI ATGCAT 2 cut(s) 118, 463
PagI TCATGA 1 cut(s) 102
PciSI GCTCTTC 1 cut(s) 11
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 426
PdmI GAANNNNTTC 1 cut(s) 324
PleI GAGTC 1 cut(s) 425
PpsI GAGTC 1 cut(s) 425
PspEI GGTNACC 1 cut(s) 403
RsaI GTAC 4 cut(s) 184, 245, 368, 490
RsaNI GTAC 4 cut(s) 183, 244, 367, 489
RseI CAYNNNNRTG 1 cut(s) 147
SapI GCTCTTC 1 cut(s) 11
SaqAI TTAA 1 cut(s) 96
Sau3AI GATC 1 cut(s) 139
SchI GAGTC 1 cut(s) 425
SduI GDGCHC 1 cut(s) 131
SetI ASST 8 cut(s) 317, 344, 355, 405, 416, 444, 480, 517
SfaNI GCATC 4 cut(s) 125, 268, 435, 448
SmiMI CAYNNNNRTG 1 cut(s) 147
Sse9I AATT 2 cut(s) 192, 324
SsiI CCGC 1 cut(s) 47
SspMI CTAG 4 cut(s) 69, 75, 312, 500
StyI CCWWGG 2 cut(s) 68, 442
TaaI ACNGT 2 cut(s) 29, 85
TaiI ACGT 1 cut(s) 416
TaqI TCGA 2 cut(s) 420, 429
TasI AATT 2 cut(s) 192, 324
TatI WGTACW 3 cut(s) 243, 366, 488
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 2 cut(s) 307, 403
Tsp45I GTSAC 2 cut(s) 307, 403
TspDTI ATGAA 2 cut(s) 189, 287
TspGWI ACGGA 1 cut(s) 484
TspRI CASTG 1 cut(s) 220
XapI RAATTY 2 cut(s) 192, 324
XmaJI CCTAGG 1 cut(s) 68
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 4 cut(s) 69, 75, 312, 500
Zsp2I ATGCAT 2 cut(s) 118, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.