MD07G1050200.v1.1

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001291
Physical Location & Seq
Reverse (-)
4296175 .. 4299868
3694 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1050200.v1.1.491

Sequence Viewer

Length: 2463 bp
ATGGCTTTTAAGTTGAATGGGACAATTGGTGGGGAAGATTTTGTAATAACCAGTAACACAAAAAATTTAATTGAGGTATGCTTCAATAAAACGATATCCCCAACAGGACTATGGCGAGCACAGAACCCCTTCATGTCAGCTCTTTCAGTTCTTGTTATAAGACTGAGCATCGCGGTCTTCAGCACTCGTATTTTTATGCTTGTCTTCAAACCCTTGCACCAACCACGGATTATTGCTGAGATTCTTGGTGGAATGTCGACGAGTACGAGTAATTGGGGTAAAGTTGTACCAGCCGTATTTCCTTATAGTACCGTGAAGATTATCGAGACCATGGGAAACTTGGCCCTCGTTTATCACATGTTCCTGGTGGGTTTAGAGCTTGATTTAAAACCAATATTACGAGCTGGGAAGAAGGCTATTAGCATTGCGCTCGCCGGCATTATCTTTTCCGTCCTTGTTGGCTGGGCCTTATGTCGATATGTACTCCTCAAAGATTTTGCCGAACAAACAAAAGGCAATGGAGGAAAACTTCCTACTAAGGATGGCCCGTGGTTTTGGGGCGTTGCTCTTGCCACCACTAACTTCCCAGACCTTGCCCGAATCCTCGCAGACCTCAAACTTCTCTACTCTGATGTCGGAGGACTTGCGTTATCTGCCTCTGTTATCTCCGACTTATGCTCCTGGTTTCTTCTTCTGCTTGTAATGGCCATAGTCAACAGCTTCCAGACAGTTGCGGTGGGACTTACCTTGCTTTTTATAGGACTATGCGTTTTTGTAGTACATCCTCTTCTGTCATGGATTGTTAACCGAGTTCGCGAAAGAGCACAAGAGGATGGCGACTACAGTCAATTTGAATGCTTTGTTCTAGCTGGGGTTGTGCTTTGTGGGTACATTACCGATGCATGTGGATCCCATTCCATCGTAGGGTCTTTTATGTTTGGAGCTATCATGCCTAGAGGAGAGGTTTCAAACAATCTTAAAAAGAAGGTGGGAAGTTTTGTGCCTGGGATTCTAATGCCTCTCTACTTCTGTATCAATGGGGGAAGACTCAACTACGTGGATATTCTCAGTTCCATTTATCCATCGGAGAAAAAAACTGGGACGGATATATATCGCGTAGGGGGGGTTGTCATATTAGCTTTTGCAGCTAAGACTGTGAGCACTTTGGTTGCCGGCTTACTCAACAAAATGTCATTTCGGGATAGTTTGGCTCTTGGAGTGCTCATGAACACCAAGGGCTTATTTACACTTATCATACTCAACACTGGTCGGGACGTAAAGGCGTTGAACAAACAAACCTTCGGAGTAATGGTGTGGGCAATTTTGGTAATGACATTTGCAGTTGGCCCGCTTTTAGTCTTCCTATACAAGTCCAATGCCAGGCCTTTTGTGCGATACAAACAGAGAAACGTAGCAAGCGTAGGATCAAACGACGAGCTTCGAATCCTTGCATGTGCTCACAACTCACGCAATGTGTCAGGCATTATCAATCTCCTTGAGGCTTCTAATGCAACCAAAAACTCCCCTCTTCATGTTCTTGCTGTCCACCTTGTGGAACTAACCGGGCATGCTTCGGCGATGTTGGTAGTGCACGGTGCTTGTAAGACCAATGATGTACACTTTCCCTACAACTCGTCGTCCCCCTCAAGTGGCTTTGAAACATATGCCAAACATAGAGAAAATGTCACTGTGCAAACACTCACGGCAGTGTCTCAATACTCCACGATGCACCGGGACATATGCAACCTGGCCGAGGAGAACCGTGTTGCCATAATCATCATCCCGTTCCACAAGAAATCCACCATATTGGTTGGAGGAGTGACACCAGATCCAAACAACTCACATCTAAGGAGGCTCAACAACAACTTGATCGCAAATGCGCAGTGCTCTGTGGGTGTTCTTGTGGATCGAGGCCTTGGTGCATTCAACTACTCTTACCCTCAATGCCGCTATTCCATGCTCTTCTTTGGAGGGGTAGATGACCGCGAGGCATTAGCGTATGTCGGTCGGATGGCTGGACATCCCAAGGTTAACCTAACAGTCATATCGTTTAATATTATGAGTGAAAATGAAGCAAAAGCGTGCTCTAATGATAATGATGATCGTACTGATGGGTTTCTGAAGGCACTGGCAAGCATTGGGAGGGAAAAAAAACTGGACGAGTTGTTCTTGGATGAGTTTAGGCTGAGGTCAATGAATGATGCCTCCATAAAATTTGTAGAGAATTGGGTGACGAGTTGGGAACAAATTCTCTCGCTAATACAATCCACGGAAGGTGAATATGATATGTTCATAGTGGGTAGAAGACATGGAGAAATGCAAGAGGCCATCAGCACAACGTTGCTGGATGACGATGACTCTAATGACATTGGAGTTCTTGGAGATGCATTGGTATCTTCAACCTTCACAGCTAGTACATCCATTCTAATTGTGCAACAAGGTAGCCATTTGGATTATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

821

Amino Acids

89.78

Weight (kDa)

8.49

Isoelectric Point (pI)

28.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 63 - 452 1.1e-48 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 511 - 641 9.6e-16 Plant cation/H(+) antiporter domain
CHX17_C PF23259 652 - 813 2e-14 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 158, 2457
Acc16I TGCGCA 1 cut(s) 1881
AccB7I CCANNNNNTGG 1 cut(s) 1552
AccI GTMKAC 1 cut(s) 257
AccII CGCG 4 cut(s) 173, 816, 1116, 1986
AciI CCGC 5 cut(s) 173, 734, 1349, 1948, 1984
AclI AACGTT 1 cut(s) 2339
AclWI GGATC 5 cut(s) 903, 916, 1432, 1823, 1914
AcoI YGGCCR 2 cut(s) 705, 1749
AcsI RAATTY 3 cut(s) 64, 2213, 2247
AcuI CTGAAG 2 cut(s) 163, 2141
AdeI CACNNNGTG 1 cut(s) 1552
AfaI GTAC 9 cut(s) 265, 288, 310, 483, 780, 890, 1617, 2107, 2416
AfiI CCNNNNNNNGG 5 cut(s) 924, 1380, 1552, 1649, 1753
AflIII ACRYGT 1 cut(s) 357
AgsI TTSAA 9 cut(s) 16, 85, 208, 854, 969, 1288, 1658, 1927, 2400
AjnI CCWGG 5 cut(s) 363, 680, 1003, 1379, 1746
AleI CACNNNNGTG 1 cut(s) 1706
AloI GAACNNNNNNTCC 2 cut(s) 2150, 2182
Alw21I GWGCWC 8 cut(s) 121, 826, 1163, 1224, 1459, 1593, 1889, 2087
Alw26I GTCTC 2 cut(s) 320, 1716
Alw44I GTGCAC 1 cut(s) 1589
AlwI GGATC 5 cut(s) 903, 916, 1432, 1823, 1914
AoxI GGCC 9 cut(s) 342, 465, 544, 705, 1345, 1382, 1749, 1912, 2325
ApaLI GTGCAC 1 cut(s) 1589
ApeKI GCWGC 1 cut(s) 1145
ApoI RAATTY 3 cut(s) 64, 2213, 2247
Asp700I GAANNNNTTC 2 cut(s) 128, 2247
AspLEI GCGC 2 cut(s) 430, 1882
AspS9I GGNCC 4 cut(s) 343, 465, 545, 1346
AsuC2I CCSGG 2 cut(s) 1564, 1733
AsuHPI GGTGA 2 cut(s) 2242, 2288
AsuII TTCGAA 1 cut(s) 1441
BaeGI GKGCMC 1 cut(s) 1593
BaeI ACNNNNGTAYC 2 cut(s) 1015, 1048
BalI TGGCCA 1 cut(s) 707
BamHI GGATCC 1 cut(s) 908
BarI GAAGNNNNNNTAC 2 cut(s) 771, 803
BbsI GAAGAC 5 cut(s) 169, 196, 1051, 1351, 2311
Bbv12I GWGCWC 8 cut(s) 121, 826, 1163, 1224, 1459, 1593, 1889, 2087
BbvCI CCTCAGC 1 cut(s) 2186
BbvI GCAGC 1 cut(s) 1157
BccI CCATC 7 cut(s) 536, 827, 926, 1090, 2005, 2105, 2336
BceAI ACGGC 2 cut(s) 278, 1719
BcgI CGANNNNNNTGC 2 cut(s) 412, 446
BciT130I CCWGG 5 cut(s) 365, 682, 1005, 1381, 1748
BcnI CCSGG 2 cut(s) 1564, 1733
BcoDI GTCTC 2 cut(s) 320, 1716
BfaI CTAG 3 cut(s) 866, 954, 2412
BfmI CTRYAG 1 cut(s) 841
BisI GCNGC 2 cut(s) 1146, 1948
BlsI GCNGC 2 cut(s) 1147, 1949
Bme1390I CCNGG 7 cut(s) 365, 682, 1005, 1381, 1564, 1733, 1748
BmgT120I GGNCC 4 cut(s) 343, 465, 545, 1346
BmiI GGNNCC 1 cut(s) 910
BmrFI CCNGG 7 cut(s) 365, 682, 1005, 1381, 1564, 1733, 1748
BmrI ACTGGG 1 cut(s) 1107
BmsI GCATC 5 cut(s) 177, 889, 1716, 2191, 2374
BmuI ACTGGG 1 cut(s) 1107
BoxI GACNNNNGTC 1 cut(s) 843
BpiI GAAGAC 5 cut(s) 169, 196, 1051, 1351, 2311
Bpu10I CCTNAGC 1 cut(s) 2186
Bpu14I TTCGAA 1 cut(s) 1441
BpuEI CTTGAG 2 cut(s) 1517, 1630
BpuMI CCSGG 2 cut(s) 1564, 1733
BsaAI YACGTR 1 cut(s) 1057
BsaBI GATNNNNATC 1 cut(s) 1110
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 9 cut(s) 224, 330, 548, 1004, 1233, 1752, 1915, 2025, 2268
BsaXI ACNNNNNCTCC 4 cut(s) 662, 692, 1296, 1326
Bsc4I CCNNNNNNNGG 5 cut(s) 924, 1380, 1552, 1649, 1753
Bse118I RCCGGY 2 cut(s) 434, 1172
Bse1I ACTGG 5 cut(s) 51, 1102, 1270, 2133, 2160
Bse3DI GCAATG 3 cut(s) 423, 523, 1477
Bse8I GATNNNNATC 1 cut(s) 1110
BseBI CCWGG 5 cut(s) 365, 682, 1005, 1381, 1748
BseDI CCNNGG 9 cut(s) 224, 330, 548, 1004, 1233, 1752, 1915, 2025, 2268
BseGI GGATG 9 cut(s) 547, 781, 838, 1779, 2016, 2020, 2179, 2353, 2417
BseJI GATNNNNATC 1 cut(s) 1110
BseLI CCNNNNNNNGG 5 cut(s) 924, 1380, 1552, 1649, 1753
BseMI GCAATG 3 cut(s) 423, 523, 1477
BseMII CTCAG 4 cut(s) 155, 228, 1081, 2177
BseNI ACTGG 5 cut(s) 51, 1102, 1270, 2133, 2160
BseRI GAGGAG 4 cut(s) 476, 972, 1769, 1830
BseSI GKGCMC 1 cut(s) 1593
BseXI GCAGC 1 cut(s) 1157
BseYI CCCAGC 3 cut(s) 404, 462, 869
Bsh1236I CGCG 4 cut(s) 173, 816, 1116, 1986
Bsh1285I CGRYCG 1 cut(s) 2008
BshFI GGCC 9 cut(s) 344, 467, 546, 707, 1347, 1384, 1751, 1914, 2327
BsiEI CGRYCG 1 cut(s) 2008
BsiHKAI GWGCWC 8 cut(s) 121, 826, 1163, 1224, 1459, 1593, 1889, 2087
BsiSI CCGG 4 cut(s) 435, 1173, 1563, 1732
BslFI GGGAC 6 cut(s) 34, 753, 1114, 1286, 1624, 1748
BslI CCNNNNNNNGG 5 cut(s) 924, 1380, 1552, 1649, 1753
BsmAI GTCTC 2 cut(s) 320, 1716
BsmFI GGGAC 6 cut(s) 34, 753, 1114, 1286, 1624, 1748
BsmI GAATGC 2 cut(s) 860, 1922
BsnI GGCC 9 cut(s) 344, 467, 546, 707, 1347, 1384, 1751, 1914, 2327
Bso31I GGTCTC 1 cut(s) 320
Bsp119I TTCGAA 1 cut(s) 1441
Bsp1286I GDGCHC 8 cut(s) 121, 826, 1163, 1224, 1459, 1593, 1889, 2087
Bsp1407I TGTACA 1 cut(s) 1615
Bsp143I GATC 6 cut(s) 908, 1424, 1828, 1869, 1906, 2101
Bsp19I CCATGG 1 cut(s) 330
Bsp68I TCGCGA 1 cut(s) 816
BspACI CCGC 5 cut(s) 173, 734, 1349, 1948, 1984
BspANI GGCC 9 cut(s) 344, 467, 546, 707, 1347, 1384, 1751, 1914, 2327
BspCNI CTCAG 4 cut(s) 156, 229, 1080, 2178
BspFNI CGCG 4 cut(s) 173, 816, 1116, 1986
BspHI TCATGA 1 cut(s) 1224
BspLI GGNNCC 1 cut(s) 910
BspPI GGATC 5 cut(s) 903, 916, 1432, 1823, 1914
BspQI GCTCTTC 1 cut(s) 1967
BspT104I TTCGAA 1 cut(s) 1441
BspTNI GGTCTC 1 cut(s) 320
BsrDI GCAATG 3 cut(s) 423, 523, 1477
BsrFI RCCGGY 2 cut(s) 434, 1172
BsrGI TGTACA 1 cut(s) 1615
BsrI ACTGG 5 cut(s) 51, 1102, 1270, 2133, 2160
BssAI RCCGGY 2 cut(s) 434, 1172
BssECI CCNNGG 9 cut(s) 224, 330, 548, 1004, 1233, 1752, 1915, 2025, 2268
BssMI GATC 6 cut(s) 908, 1424, 1828, 1869, 1906, 2101
BssT1I CCWWGG 4 cut(s) 330, 1233, 1915, 2025
Bst2UI CCWGG 5 cut(s) 365, 682, 1005, 1381, 1748
Bst4CI ACNGT 8 cut(s) 313, 730, 845, 1156, 1595, 1690, 1763, 2041
Bst6I CTCTTC 3 cut(s) 792, 1533, 1967
BstAUI TGTACA 1 cut(s) 1615
BstBAI YACGTR 1 cut(s) 1057
BstBI TTCGAA 1 cut(s) 1441
BstC8I GCNNGC 9 cut(s) 117, 432, 436, 1174, 1349, 1417, 1569, 2083, 2134
BstDEI CTNAG 7 cut(s) 164, 237, 537, 1067, 1149, 1847, 2186
BstDSI CCRYGG 4 cut(s) 224, 330, 548, 2268
BstENI CCTNNNNNAGG 1 cut(s) 1751
BstF5I GGATG 9 cut(s) 547, 781, 838, 1779, 2016, 2020, 2179, 2353, 2417
BstFNI CGCG 4 cut(s) 173, 816, 1116, 1986
BstHHI GCGC 2 cut(s) 430, 1882
BstKTI GATC 6 cut(s) 911, 1427, 1831, 1872, 1909, 2104
BstMAI GTCTC 2 cut(s) 320, 1716
BstMBI GATC 6 cut(s) 908, 1424, 1828, 1869, 1906, 2101
BstMCI CGRYCG 1 cut(s) 2008
BstMWI GCNNNNNNNGC 4 cut(s) 653, 1145, 1390, 1508
BstNI CCWGG 5 cut(s) 365, 682, 1005, 1381, 1748
BstNSI RCATGY 4 cut(s) 361, 906, 1455, 1571
BstPAI GACNNNNGTC 1 cut(s) 843
BstSCI CCNGG 7 cut(s) 363, 680, 1003, 1379, 1562, 1731, 1746
BstSFI CTRYAG 1 cut(s) 841
BstSLI GKGCMC 1 cut(s) 1593
BstUI CGCG 4 cut(s) 173, 816, 1116, 1986
BstV1I GCAGC 1 cut(s) 1157
BstV2I GAAGAC 5 cut(s) 169, 196, 1051, 1351, 2311
BstX2I RGATCY 2 cut(s) 908, 1828
BstXI CCANNNNNNTGG 1 cut(s) 1807
BstYI RGATCY 2 cut(s) 908, 1828
BsuRI GGCC 9 cut(s) 344, 467, 546, 707, 1347, 1384, 1751, 1914, 2327
BtgI CCRYGG 4 cut(s) 224, 330, 548, 2268
BtgZI GCGATG 2 cut(s) 154, 1592
BtsCI GGATG 9 cut(s) 547, 781, 838, 1779, 2016, 2020, 2179, 2353, 2417
BtsI GCAGTG 2 cut(s) 1713, 1889
BtsIMutI CAGTG 5 cut(s) 1263, 1686, 1713, 1889, 2126
BtuMI TCGCGA 1 cut(s) 816
Cac8I GCNNGC 9 cut(s) 117, 432, 436, 1174, 1349, 1417, 1569, 2083, 2134
CciI TCATGA 1 cut(s) 1224
CfoI GCGC 2 cut(s) 430, 1882
Cfr10I RCCGGY 2 cut(s) 434, 1172
Cfr13I GGNCC 4 cut(s) 343, 465, 545, 1346
Csp6I GTAC 9 cut(s) 264, 287, 309, 482, 779, 889, 1616, 2106, 2415
CviQI GTAC 9 cut(s) 264, 287, 309, 482, 779, 889, 1616, 2106, 2415
DdeI CTNAG 7 cut(s) 164, 237, 537, 1067, 1149, 1847, 2186
DpnI GATC 6 cut(s) 910, 1426, 1830, 1871, 1908, 2103
DpnII GATC 6 cut(s) 908, 1424, 1828, 1869, 1906, 2101
DraI TTTAAA 1 cut(s) 387
DraIII CACNNNGTG 1 cut(s) 1552
EaeI YGGCCR 2 cut(s) 705, 1749
Eam1104I CTCTTC 3 cut(s) 792, 1533, 1967
EarI CTCTTC 3 cut(s) 792, 1533, 1967
Eco130I CCWWGG 4 cut(s) 330, 1233, 1915, 2025
Eco147I AGGCCT 2 cut(s) 1384, 1914
Eco31I GGTCTC 1 cut(s) 320
Eco32I GATATC 1 cut(s) 96
Eco57I CTGAAG 2 cut(s) 163, 2141
EcoNI CCTNNNNNAGG 1 cut(s) 1751
EcoRII CCWGG 5 cut(s) 363, 680, 1003, 1379, 1746
EcoRV GATATC 1 cut(s) 96
EcoT14I CCWWGG 4 cut(s) 330, 1233, 1915, 2025
EcoT22I ATGCAT 2 cut(s) 904, 2389
ErhI CCWWGG 4 cut(s) 330, 1233, 1915, 2025
FaqI GGGAC 6 cut(s) 34, 753, 1114, 1286, 1624, 1748
FauI CCCGC 1 cut(s) 1356
FauNDI CATATG 2 cut(s) 1663, 1739
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 2 cut(s) 1146, 1948
FokI GGATG 9 cut(s) 554, 768, 845, 1766, 2007, 2023, 2186, 2360, 2404
Fsp4HI GCNGC 2 cut(s) 1146, 1948
FspBI CTAG 3 cut(s) 866, 954, 2412
FspI TGCGCA 1 cut(s) 1881
GlaI GCGC 2 cut(s) 429, 1881
GluI GCNGC 2 cut(s) 1146, 1948
GsaI CCCAGC 3 cut(s) 408, 466, 873
HaeIII GGCC 9 cut(s) 344, 467, 546, 707, 1347, 1384, 1751, 1914, 2327
HapII CCGG 4 cut(s) 435, 1173, 1563, 1732
HhaI GCGC 2 cut(s) 430, 1882
Hin6I GCGC 2 cut(s) 428, 1880
HinP1I GCGC 2 cut(s) 428, 1880
HincII GTYRAC 4 cut(s) 258, 715, 805, 2032
HindII GTYRAC 4 cut(s) 258, 715, 805, 2032
HinfI GANTC 6 cut(s) 241, 600, 1009, 1047, 1443, 2357
HpaI GTTAAC 2 cut(s) 805, 2032
HpaII CCGG 4 cut(s) 435, 1173, 1563, 1732
HphI GGTGA 2 cut(s) 2242, 2288
Hpy166II GTNNAC 7 cut(s) 258, 715, 805, 1546, 1591, 1618, 2032
Hpy188I TCNGA 7 cut(s) 631, 638, 670, 1087, 1304, 2010, 2121
Hpy188III TCNNGA 6 cut(s) 325, 724, 815, 1199, 1225, 1271
Hpy8I GTNNAC 7 cut(s) 258, 715, 805, 1546, 1591, 1618, 2032
Hpy99I CGWCG 3 cut(s) 262, 1436, 1639
HpyAV CCTTC 7 cut(s) 139, 406, 979, 1309, 2116, 2267, 2413
HpyCH4III ACNGT 8 cut(s) 313, 730, 845, 1156, 1595, 1690, 1763, 2041
HpyCH4IV ACGT 4 cut(s) 1056, 1275, 1410, 2339
HpyF10VI GCNNNNNNNGC 4 cut(s) 653, 1145, 1390, 1508
HpyF3I CTNAG 7 cut(s) 164, 237, 537, 1067, 1149, 1847, 2186
HpySE526I ACGT 4 cut(s) 1056, 1275, 1410, 2339
HspAI GCGC 2 cut(s) 428, 1880
KroI GCCGGC 2 cut(s) 434, 1172
KroNI GCCGGC 2 cut(s) 436, 1174
KspAI GTTAAC 2 cut(s) 805, 2032
Kzo9I GATC 6 cut(s) 908, 1424, 1828, 1869, 1906, 2101
LguI GCTCTTC 1 cut(s) 1967
LmnI GCTCC 2 cut(s) 683, 941
Lsp1109I GCAGC 1 cut(s) 1157
LweI GCATC 5 cut(s) 177, 889, 1716, 2191, 2374
MaeI CTAG 3 cut(s) 866, 954, 2412
MaeII ACGT 4 cut(s) 1056, 1275, 1410, 2339
MaeIII GTNAC 4 cut(s) 53, 1684, 1819, 2230
MalI GATC 6 cut(s) 910, 1426, 1830, 1871, 1908, 2103
MboI GATC 6 cut(s) 908, 1424, 1828, 1869, 1906, 2101
MfeI CAATTG 1 cut(s) 24
MflI RGATCY 2 cut(s) 908, 1828
MhlI GDGCHC 8 cut(s) 121, 826, 1163, 1224, 1459, 1593, 1889, 2087
MlsI TGGCCA 1 cut(s) 707
MluNI TGGCCA 1 cut(s) 707
MlyI GAGTC 2 cut(s) 1041, 2351
MmeI TCCRAC 4 cut(s) 616, 693, 1792, 1988
Mox20I TGGCCA 1 cut(s) 707
Mph1103I ATGCAT 2 cut(s) 904, 2389
MroNI GCCGGC 2 cut(s) 434, 1172
MroXI GAANNNNTTC 2 cut(s) 128, 2247
MscI TGGCCA 1 cut(s) 707
MseI TTAA 7 cut(s) 9, 68, 386, 804, 978, 2031, 2052
MslI CAYNNNNRTG 1 cut(s) 1706
Msp20I TGGCCA 1 cut(s) 707
MspI CCGG 4 cut(s) 435, 1173, 1563, 1732
MspR9I CCNGG 7 cut(s) 365, 682, 1005, 1381, 1564, 1733, 1748
MunI CAATTG 1 cut(s) 24
Mva1269I GAATGC 2 cut(s) 860, 1922
MvaI CCWGG 5 cut(s) 365, 682, 1005, 1381, 1748
MvnI CGCG 4 cut(s) 173, 816, 1116, 1986
MwoI GCNNNNNNNGC 4 cut(s) 653, 1145, 1390, 1508
NaeI GCCGGC 2 cut(s) 436, 1174
NciI CCSGG 2 cut(s) 1564, 1733
NcoI CCATGG 1 cut(s) 330
NdeI CATATG 2 cut(s) 1663, 1739
NdeII GATC 6 cut(s) 908, 1424, 1828, 1869, 1906, 2101
NgoMIV GCCGGC 2 cut(s) 434, 1172
NlaIV GGNNCC 1 cut(s) 910
NmeAIII GCCGAG 1 cut(s) 1777
NmuCI GTSAC 3 cut(s) 1684, 1819, 2230
NruI TCGCGA 1 cut(s) 816
NsbI TGCGCA 1 cut(s) 1881
NsiI ATGCAT 2 cut(s) 904, 2389
NspI RCATGY 4 cut(s) 361, 906, 1455, 1571
NspV TTCGAA 1 cut(s) 1441
OliI CACNNNNGTG 1 cut(s) 1706
PaeI GCATGC 1 cut(s) 1571
PagI TCATGA 1 cut(s) 1224
PceI AGGCCT 2 cut(s) 1384, 1914
PciI ACATGT 1 cut(s) 357
PciSI GCTCTTC 1 cut(s) 1967
PcsI WCGNNNNNNNCGW 2 cut(s) 263, 1416
PctI GAATGC 2 cut(s) 860, 1922
PdiI GCCGGC 2 cut(s) 436, 1174
PdmI GAANNNNTTC 2 cut(s) 128, 2247
PfeI GAWTC 4 cut(s) 241, 600, 1009, 1443
PflMI CCANNNNNTGG 1 cut(s) 1552
PkrI GCNGC 2 cut(s) 1147, 1949
PleI GAGTC 2 cut(s) 1041, 2351
PpsI GAGTC 2 cut(s) 1041, 2351
Ppu21I YACGTR 1 cut(s) 1057
PscI ACATGT 1 cut(s) 357
PshAI GACNNNNGTC 1 cut(s) 843
PsiI TTATAA 2 cut(s) 158, 2457
Psp1406I AACGTT 1 cut(s) 2339
Psp6I CCWGG 5 cut(s) 363, 680, 1003, 1379, 1746
PspFI CCCAGC 3 cut(s) 404, 462, 869
PspGI CCWGG 5 cut(s) 363, 680, 1003, 1379, 1746
PspN4I GGNNCC 1 cut(s) 910
PspPI GGNCC 4 cut(s) 343, 465, 545, 1346
PsuI RGATCY 2 cut(s) 908, 1828
RruI TCGCGA 1 cut(s) 816
RsaI GTAC 9 cut(s) 265, 288, 310, 483, 780, 890, 1617, 2107, 2416
RsaNI GTAC 9 cut(s) 264, 287, 309, 482, 779, 889, 1616, 2106, 2415
RseI CAYNNNNRTG 1 cut(s) 1706
SalI GTCGAC 1 cut(s) 256
SapI GCTCTTC 1 cut(s) 1967
SaqAI TTAA 7 cut(s) 9, 68, 386, 804, 978, 2031, 2052
SatI GCNGC 2 cut(s) 1146, 1948
Sau3AI GATC 6 cut(s) 908, 1424, 1828, 1869, 1906, 2101
Sau96I GGNCC 4 cut(s) 343, 465, 545, 1346
SchI GAGTC 2 cut(s) 1041, 2351
ScrFI CCNGG 7 cut(s) 365, 682, 1005, 1381, 1564, 1733, 1748
SduI GDGCHC 8 cut(s) 121, 826, 1163, 1224, 1459, 1593, 1889, 2087
SfaNI GCATC 5 cut(s) 177, 889, 1716, 2191, 2374
SfcI CTRYAG 1 cut(s) 841
SfuI TTCGAA 1 cut(s) 1441
SmiMI CAYNNNNRTG 1 cut(s) 1706
SmlI CTYRAG 2 cut(s) 1496, 1645
SmoI CTYRAG 2 cut(s) 1496, 1645
SphI GCATGC 1 cut(s) 1571
SseBI AGGCCT 2 cut(s) 1384, 1914
SsiI CCGC 5 cut(s) 173, 734, 1349, 1948, 1984
SspI AATATT 2 cut(s) 396, 2056
SspMI CTAG 3 cut(s) 866, 954, 2412
StuI AGGCCT 2 cut(s) 1384, 1914
StyD4I CCNGG 7 cut(s) 363, 680, 1003, 1379, 1562, 1731, 1746
StyI CCWWGG 4 cut(s) 330, 1233, 1915, 2025
TaaI ACNGT 8 cut(s) 313, 730, 845, 1156, 1595, 1690, 1763, 2041
TaiI ACGT 4 cut(s) 1059, 1278, 1413, 2342
TaqI TCGA 5 cut(s) 257, 324, 475, 1441, 1909
TaqII GACCGA 1 cut(s) 1994
TatI WGTACW 4 cut(s) 481, 778, 1615, 2414
TauI GCSGC 1 cut(s) 1950
TfiI GAWTC 4 cut(s) 241, 600, 1009, 1443
Tru1I TTAA 7 cut(s) 9, 68, 386, 804, 978, 2031, 2052
Tru9I TTAA 7 cut(s) 9, 68, 386, 804, 978, 2031, 2052
TscAI CASTG 5 cut(s) 1270, 1693, 1713, 1889, 2133
TseFI GTSAC 3 cut(s) 1684, 1819, 2230
TseI GCWGC 1 cut(s) 1145
Tsp45I GTSAC 3 cut(s) 1684, 1819, 2230
TspDTI ATGAA 6 cut(s) 121, 1241, 1520, 2085, 2210, 2281
TspGWI ACGGA 4 cut(s) 241, 439, 1118, 2285
TspRI CASTG 5 cut(s) 1270, 1693, 1713, 1889, 2133
Van91I CCANNNNNTGG 1 cut(s) 1552
VneI GTGCAC 1 cut(s) 1589
XagI CCTNNNNNAGG 1 cut(s) 1751
XapI RAATTY 3 cut(s) 64, 2213, 2247
XceI RCATGY 4 cut(s) 361, 906, 1455, 1571
XcmI CCANNNNNNNNNTGG 2 cut(s) 108, 337
XmiI GTMKAC 1 cut(s) 257
XmnI GAANNNNTTC 2 cut(s) 128, 2247
XspI CTAG 3 cut(s) 866, 954, 2412
Zsp2I ATGCAT 2 cut(s) 904, 2389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.