Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001291

Physical Location & Seq

Genomic Coordinates Reverse (-)

4321724 .. 4324985

Gene Structure 3262 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1050300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2502 bp

ATGGCGCAAAAAAATTTAATTAGTGGTGGGGAACAGTTTGATTTATATTTTGACACAAAAGATAGAATAATGGTGTGCTATAATAGAACGATAAACCCAGGTATTGGGTGGCGAGCGCTACCCAGCGAATCCTTCGTCTCACCTCTTGGCGTTCTTATTATAAAACTGAGCATCGCTGTCTTCAACACTCATATTCTTAAATTCGCCTTCAAACCCTTGCACCAACCAAGCATTGTTGCTCAGATTATTGCTGGAATGACAACGAGTACGAGGGCTTTGGGTAGATTCACGAAACCTATATTCCCTTTTAGTACCTTGAAGGTTACCGAGACCATGGGAAACTTGGCCCTCGTTTACTATATGTTTCTGGTGGGGTTAGAGCTTGATTTTAAACCAGTAGTACGTGCTGGGAAGAAGGCTCTCAGCATTGCACTCACTGGCTTTTTCTTTTCTGTCCTTGTTGGTTGGGTCTTGTGTCGATATTTACTCCTCCAGGATTTTGTCGCACAAACACATGACGGAGATCAGGAACGTCCCACTATAAACGGCCCACTGTTCTGGGGCGTCGCTCTCGCCACCACCAATTTCCCGGACCTTGCCCGAATCCTCGCGGACCTCAAACTTCTCTACTCTGATGTCGGAGGACTTGCATTATCCGCCTCTGTTATCACCGACTTGTGCTCCTGGATTCTTCTTTTGCTCGTAATGGCCATAATCAACAGCCTCCAGATGTATGCAGTGGCCTTGACCTTGGCTTTTATAGGACTTTGCGTTTTTGCAGTACGTCCAGCTCTGTCATGGATTGTTCACCGAGTATGCGAAAGAGAACAAGAGGATAGGAACAAGAGTCAGCTTATATGCTTTGTTTTAGCTGGAGTGTTGCTCTCTGGGATCATTACCGATGCCTGTGGATCCCATTCCATTGTAGGGCCTTTTGTGTTAGGAGCCATTATGCCTAAGGGAGAGTTTACAGACATGCTTAAAGAGAAGGTGGGAAATTTTGTGCCTGCGATTCTCATGCCTCTATACTTCTGTATCAATGGAGGAAGAGTCAACTATGAGGATATTCTCGGTGACCAAAAGAATCCAATGGAGCGAAAAAGTGGGACTACCCTCGGTCGTGTAGTGTGGGTTACCATCATAGCTTTCGCAACTAAAACTGTGAGCACTTTCGTTGCCGGCATAATCAACAAAATGTCACCCCGGGATAGTCTGGCTCTTGGAGTACTTATGAACACCAAAGGCTTATTGACGCTTATTATACTCAACAGTGGCCGGGACATAAAGGCATTGAACAAACAAACGTTCGGGGTATTGATGGTGGTGATTTGGGTAATGACATTTGCGGTTGGCCCGTTTATAGCCTTCTTTTACAAGTCCAGTGTCAAGACTTTCGTGCAATACAAACAGAGGAGCGTAGCAAGCGTAGGACCTAAGAACGAGCTTCGAGTCCTTGCATGCGCTCACACCTCACGCAATGTGCCAGGCATTATCAACCTCCTTGAGGCGTCTAATCCAACTCAACAATCCCCTCTTCATGTTCTTGCTGTCCACCTTGTGGAACTAACTGGACACGCTTCGGCGATGTTGGTAGTGCACGATGCTTGCAAGACCAAAGATGTAAACTTTGTTGATAAATCGTCATCTCCGTCAAATGCCATTGAGATATATGCCAAACGAAGGGAAAGCGTCACGGTGAAATCACTCACGGCGGTGTCTCCTTACTCGACGATGCACGAGGACATATGTAACCTGGCGGAGGACAATCGAGTCTCCATAATAATTATCCCGTACCATAAGAAAGCTACAATATTGGACGGAGGAGGAGGGGCACAAGATGAAGACAGCTCGCATTTAAAGAACATCAACAACAACTTGATGGCAAATGCGCGGTGTTCCGTGGGTGTTCTTGTAGATCGGGGCCTCGGTGCATCCAACTACTTCGACTGCCGCCGCCACTTTACCATGCTCTTCTTTGGAGGGGTAGATGACCGTGAGGCATTAGCGTATGCGGGGAGGATGGCTGGACATCCAAGGGTTACCCTAACAGTTACAACGTTTAACATCATGAGCAAAGAGGCCGAAAAAACATGCATCGATGATGACGACAGTGATGAGTATACTGATGATGACGACGACGAGGATGATGACGATGTTACTCTTGAGGCAATGAAAAGTACTGGAGAGGAAAAAAAAATGGATGATTTGTATTTGGATGAGTTTAGGTTGAGGTCAATGAATGATGCCTCCATAAAATTTGTAGAGAATTGGGTGAGGAGTTGGGAACAAATTCTCTTGCTAATACAATCCATGGAGGGTGAATATGATATGTTCATAGTAGGAAGAAGACATGGAGAAATACAAGAGGTCACCACAACGGTACTGGATGATGATGACTCTAATGACATTGGAGTTCTTGGAGAGGCATTGGTATCTTCAACCTTCACAGCTAGTACATCGATTTTAATTGTGCAAAAAAGTGAATTTGCTCATGATTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

834

Amino Acids

91.88

Weight (kDa)

5.66

Isoelectric Point (pI)

37.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	66 - 455	4.4e-48	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	513 - 645	1e-17	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	655 - 686	5.8e-07	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001291)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g25710 FvH4_5g31470 FvH4_7g01800
malus_domestica	MD02G1284400.v1.1 MD02G1284600.v1.1 MD02G1284900.v1.1 MD02G1285000.v1.1 MD02G1285100.v1.1 MD02G1296900.v1.1 MD07G1050200.v1.1 MD07G1050300.v1.1 MD15G1139600.v1.1
prunus_persica	Prupe.2G027500_v2.0.a1 Prupe.2G038300_v2.0.a1 Prupe.2G040900_v2.0.a1 Prupe.4G283300_v2.0.a1 Prupe.4G283400_v2.0.a1 Prupe.4G283500_v2.0.a1 Prupe.4G283500_v2.0.a1 Prupe.4G284600_v2.0.a1 Prupe.4G284900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0318411 RchiOBHm_Chr5g0030741 RchiOBHm_Chr7g0226891
rosa_laevigata	RLG00000001685 RLG00000030540 RLG00000033248
rosa_multiflora	Rmu_co8328645.1_g000001 Rmu_co8411059.1_g000001 Rmu_sc0002662.1_g000016 Rmu_sc0003238.1_g000035 Rmu_sc0003936.1_g000039 Rmu_sc0034904.1_g000001
rosa_roxburghii	Rroxscaffold_3G00232440 Rroxscaffold_4G00329260
rosa_rugosa	Rorug01G0021100 Rorug05G0120500 Rorug07G0240000.1
rosa_samantha	Rh1AG033200 Rh1BG029000 Rh1CG031500 Rh1DG038000 Rh5AG212000 Rh5BG211100 Rh5CG234800 Rh5DG216300 Rh7AG387600 Rh7BG374100 Rh7CG407200 Rh7DG386300
rosa_wichuraiana	Rw0G000940 Rw0G000950 Rw1G002660 Rw5G019460 Rw7G032560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	161
AasI	GACNNNNNNGTC	1 cut(s)	1769
AccB7I	CCANNNNNTGG	2 cut(s)	104, 1558
AccI	GTMKAC	1 cut(s)	2120
AccII	CGCG	2 cut(s)	611, 1891
AciI	CCGC	9 cut(s)	611, 657, 1346, 1712, 1757, 1891, 1951, 1954, 2012
AclI	AACGTT	2 cut(s)	1304, 2057
AclWI	GGATC	3 cut(s)	899, 906, 919
AcoI	YGGCCR	2 cut(s)	708, 1273
AcsI	RAATTY	6 cut(s)	13, 200, 997, 2255, 2289, 2483
AcyI	GRCGYC	2 cut(s)	564, 1508
AdeI	CACNNNGTG	1 cut(s)	1558
AfaI	GTAC	9 cut(s)	268, 313, 402, 783, 1227, 1793, 2179, 2382, 2455
AfeI	AGCGCT	1 cut(s)	117
AfiI	CCNNNNNNNGG	6 cut(s)	104, 927, 1504, 1558, 1680, 1759
AgsI	TTSAA	5 cut(s)	184, 211, 319, 1294, 2439
AjnI	CCWGG	5 cut(s)	97, 492, 683, 1483, 1752
AleI	CACNNNNGTG	1 cut(s)	1712
AluBI	AGCT	9 cut(s)	382, 791, 853, 872, 1145, 1444, 1805, 1848, 2450
AluI	AGCT	9 cut(s)	382, 791, 853, 872, 1145, 1444, 1805, 1848, 2450
Alw21I	GWGCWC	3 cut(s)	683, 1169, 1599
Alw26I	GTCTC	4 cut(s)	142, 323, 1722, 1777
Alw44I	GTGCAC	1 cut(s)	1595
AlwI	GGATC	3 cut(s)	899, 906, 919
Ama87I	CYCGRG	1 cut(s)	1203
Aor51HI	AGCGCT	1 cut(s)	117
AoxI	GGCC	9 cut(s)	345, 547, 708, 741, 929, 1273, 1351, 1921, 2079
ApaLI	GTGCAC	1 cut(s)	1595
ApoI	RAATTY	6 cut(s)	13, 200, 997, 2255, 2289, 2483
ArsI	GACNNNNNNTTYG	2 cut(s)	120, 152
Asp700I	GAANNNNTTC	1 cut(s)	2289
AspLEI	GCGC	4 cut(s)	7, 118, 1463, 1891
AspS9I	GGNCC	8 cut(s)	346, 548, 592, 613, 929, 1352, 1430, 1921
AsuC2I	CCSGG	4 cut(s)	590, 1204, 1205, 1277
AvaI	CYCGRG	1 cut(s)	1203
AvaII	GGWCC	3 cut(s)	592, 613, 1430
AxyI	CCTNAGG	1 cut(s)	957
BaeGI	GKGCMC	2 cut(s)	1599, 1834
BaeI	ACNNNNGTAYC	2 cut(s)	1018, 1051
BalI	TGGCCA	1 cut(s)	710
BamHI	GGATCC	1 cut(s)	911
BauI	CACGAG	1 cut(s)	1736
BbsI	GAAGAC	3 cut(s)	172, 1848, 2353
Bbv12I	GWGCWC	3 cut(s)	683, 1169, 1599
BccI	CCATC	4 cut(s)	1145, 1312, 1873, 2014
BceAI	ACGGC	2 cut(s)	562, 1725
BcgI	CGANNNNNNTGC	2 cut(s)	1000, 1034
BciT130I	CCWGG	5 cut(s)	99, 494, 685, 1485, 1754
BcnI	CCSGG	4 cut(s)	590, 1204, 1205, 1277
BcoDI	GTCTC	4 cut(s)	142, 323, 1722, 1777
BfaI	CTAG	1 cut(s)	2451
BfoI	RGCGCY	1 cut(s)	119
BisI	GCNGC	2 cut(s)	1951, 1954
BlsI	GCNGC	2 cut(s)	1952, 1955
BmcAI	AGTACT	2 cut(s)	1227, 2179
Bme1390I	CCNGG	9 cut(s)	99, 494, 590, 685, 1204, 1205, 1277, 1485, 1754
Bme18I	GGWCC	3 cut(s)	592, 613, 1430
BmeT110I	CYCGRG	1 cut(s)	1203
BmgT120I	GGNCC	8 cut(s)	346, 548, 592, 613, 929, 1352, 1430, 1921
BmiI	GGNNCC	3 cut(s)	913, 946, 1922
BmrFI	CCNGG	9 cut(s)	99, 494, 590, 685, 1204, 1205, 1277, 1485, 1754
BmsI	GCATC	7 cut(s)	180, 892, 1591, 1722, 1940, 2103, 2233
BpiI	GAAGAC	3 cut(s)	172, 1848, 2353
BplI	GAGNNNNNCTC	2 cut(s)	867, 899
BpmI	CTGGAG	4 cut(s)	476, 710, 894, 2202
BpuEI	CTTGAG	2 cut(s)	1523, 2183
BpuMI	CCSGG	4 cut(s)	590, 1204, 1205, 1277
Bsa29I	ATCGAT	2 cut(s)	2097, 2459
BsaAI	YACGTR	1 cut(s)	404
BsaHI	GRCGYC	2 cut(s)	564, 1508
BsaI	GGTCTC	1 cut(s)	323
BsaXI	ACNNNNNCTCC	4 cut(s)	665, 695, 1035, 1065
Bsc4I	CCNNNNNNNGG	6 cut(s)	104, 927, 1504, 1558, 1680, 1759
Bse118I	RCCGGY	1 cut(s)	1178
Bse1I	ACTGG	6 cut(s)	395, 442, 1380, 1573, 2185, 2388
Bse21I	CCTNAGG	1 cut(s)	957
Bse3DI	GCAATG	3 cut(s)	426, 1483, 2175
BseBI	CCWGG	5 cut(s)	99, 494, 685, 1485, 1754
BseCI	ATCGAT	2 cut(s)	2097, 2459
BseGI	GGATG	7 cut(s)	1931, 2025, 2029, 2149, 2206, 2221, 2392
BseLI	CCNNNNNNNGG	6 cut(s)	104, 927, 1504, 1558, 1680, 1759
BseMI	GCAATG	3 cut(s)	426, 1483, 2175
BseMII	CTCAG	3 cut(s)	158, 254, 436
BseNI	ACTGG	6 cut(s)	395, 442, 1380, 1573, 2185, 2388
BseRI	GAGGAG	5 cut(s)	479, 1426, 1836, 1839, 2290
BseSI	GKGCMC	2 cut(s)	1599, 1834
BseYI	CCCAGC	2 cut(s)	122, 407
Bsh1236I	CGCG	2 cut(s)	611, 1891
Bsh1285I	CGRYCG	1 cut(s)	1120
BshFI	GGCC	9 cut(s)	347, 549, 710, 743, 931, 1275, 1353, 1923, 2081
BshVI	ATCGAT	2 cut(s)	2097, 2459
BsiEI	CGRYCG	1 cut(s)	1120
BsiHKAI	GWGCWC	3 cut(s)	683, 1169, 1599
BsiHKCI	CYCGRG	1 cut(s)	1203
BsiSI	CCGG	4 cut(s)	590, 1179, 1204, 1276
BslFI	GGGAC	3 cut(s)	519, 1120, 1292
BslI	CCNNNNNNNGG	6 cut(s)	104, 927, 1504, 1558, 1680, 1759
BsmAI	GTCTC	4 cut(s)	142, 323, 1722, 1777
BsmBI	CGTCTC	1 cut(s)	142
BsmFI	GGGAC	3 cut(s)	519, 1120, 1292
BsnI	GGCC	9 cut(s)	347, 549, 710, 743, 931, 1275, 1353, 1923, 2081
Bso31I	GGTCTC	1 cut(s)	323
BsoBI	CYCGRG	1 cut(s)	1203
Bsp1286I	GDGCHC	4 cut(s)	683, 1169, 1599, 1834
Bsp143I	GATC	4 cut(s)	523, 891, 911, 1915
Bsp19I	CCATGG	2 cut(s)	333, 2310
BspACI	CCGC	9 cut(s)	611, 657, 1346, 1712, 1757, 1891, 1951, 1954, 2012
BspANI	GGCC	9 cut(s)	347, 549, 710, 743, 931, 1275, 1353, 1923, 2081
BspCNI	CTCAG	3 cut(s)	159, 253, 435
BspDI	ATCGAT	2 cut(s)	2097, 2459
BspFNI	CGCG	2 cut(s)	611, 1891
BspHI	TCATGA	2 cut(s)	2067, 2491
BspLI	GGNNCC	3 cut(s)	913, 946, 1922
BspPI	GGATC	3 cut(s)	899, 906, 919
BspQI	GCTCTTC	1 cut(s)	1976
BspTNI	GGTCTC	1 cut(s)	323
BsrDI	GCAATG	3 cut(s)	426, 1483, 2175
BsrFI	RCCGGY	1 cut(s)	1178
BsrI	ACTGG	6 cut(s)	395, 442, 1380, 1573, 2185, 2388
BssAI	RCCGGY	1 cut(s)	1178
BssMI	GATC	4 cut(s)	523, 891, 911, 1915
BssNAI	GTATAC	1 cut(s)	2121
BssNI	GRCGYC	2 cut(s)	564, 1508
BssSI	CACGAG	1 cut(s)	1736
BssT1I	CCWWGG	4 cut(s)	333, 750, 2033, 2310
Bst1107I	GTATAC	1 cut(s)	2121
Bst2BI	CACGAG	1 cut(s)	1736
Bst2UI	CCWGG	5 cut(s)	99, 494, 685, 1485, 1754
Bst4CI	ACNGT	9 cut(s)	36, 555, 1162, 1271, 1696, 1994, 2050, 2111, 2380
Bst6I	CTCTTC	3 cut(s)	1042, 1539, 1976
BstACI	GRCGYC	2 cut(s)	564, 1508
BstBAI	YACGTR	1 cut(s)	404
BstC8I	GCNNGC	7 cut(s)	114, 1008, 1180, 1423, 1459, 1606, 1850
BstDEI	CTNAG	5 cut(s)	167, 240, 422, 957, 1434
BstDSI	CCRYGG	3 cut(s)	333, 1899, 2310
BstEII	GGTNACC	5 cut(s)	322, 1073, 1132, 2038, 2368
BstENI	CCTNNNNNAGG	2 cut(s)	1502, 1757
BstF5I	GGATG	7 cut(s)	1931, 2025, 2029, 2149, 2206, 2221, 2392
BstFNI	CGCG	2 cut(s)	611, 1891
BstH2I	RGCGCY	1 cut(s)	119
BstHHI	GCGC	4 cut(s)	7, 118, 1463, 1891
BstKTI	GATC	4 cut(s)	526, 894, 914, 1918
BstMAI	GTCTC	4 cut(s)	142, 323, 1722, 1777
BstMBI	GATC	4 cut(s)	523, 891, 911, 1915
BstMCI	CGRYCG	1 cut(s)	1120
BstMWI	GCNNNNNNNGC	2 cut(s)	656, 1422
BstNI	CCWGG	5 cut(s)	99, 494, 685, 1485, 1754
BstNSI	RCATGY	3 cut(s)	979, 1461, 2094
BstPI	GGTNACC	5 cut(s)	322, 1073, 1132, 2038, 2368
BstSCI	CCNGG	9 cut(s)	97, 492, 588, 683, 1202, 1203, 1275, 1483, 1752
BstSLI	GKGCMC	2 cut(s)	1599, 1834
BstUI	CGCG	2 cut(s)	611, 1891
BstV2I	GAAGAC	3 cut(s)	172, 1848, 2353
BstX2I	RGATCY	1 cut(s)	911
BstXI	CCANNNNNNTGG	1 cut(s)	558
BstYI	RGATCY	1 cut(s)	911
BstZ17I	GTATAC	1 cut(s)	2121
Bsu15I	ATCGAT	2 cut(s)	2097, 2459
Bsu36I	CCTNAGG	1 cut(s)	957
BsuRI	GGCC	9 cut(s)	347, 549, 710, 743, 931, 1275, 1353, 1923, 2081
BsuTUI	ATCGAT	2 cut(s)	2097, 2459
BtgI	CCRYGG	3 cut(s)	333, 1899, 2310
BtgZI	GCGATG	2 cut(s)	157, 1598
BtsCI	GGATG	7 cut(s)	1931, 2025, 2029, 2149, 2206, 2221, 2392
BtsI	GCAGTG	1 cut(s)	744
BtsIMutI	CAGTG	6 cut(s)	435, 551, 744, 1276, 1387, 2116
Cac8I	GCNNGC	7 cut(s)	114, 1008, 1180, 1423, 1459, 1606, 1850
CciI	TCATGA	2 cut(s)	2067, 2491
CfoI	GCGC	4 cut(s)	7, 118, 1463, 1891
Cfr10I	RCCGGY	1 cut(s)	1178
Cfr13I	GGNCC	8 cut(s)	346, 548, 592, 613, 929, 1352, 1430, 1921
Cfr9I	CCCGGG	1 cut(s)	1203
ClaI	ATCGAT	2 cut(s)	2097, 2459
CseI	GACGC	4 cut(s)	553, 1261, 1497, 1678
Csp6I	GTAC	9 cut(s)	267, 312, 401, 782, 1226, 1792, 2178, 2381, 2454
CviQI	GTAC	9 cut(s)	267, 312, 401, 782, 1226, 1792, 2178, 2381, 2454
DdeI	CTNAG	5 cut(s)	167, 240, 422, 957, 1434
DpnI	GATC	4 cut(s)	525, 893, 913, 1917
DpnII	GATC	4 cut(s)	523, 891, 911, 1915
DraI	TTTAAA	2 cut(s)	391, 1857
DraIII	CACNNNGTG	1 cut(s)	1558
DrdI	GACNNNNNNGTC	1 cut(s)	1769
DseDI	GACNNNNNNGTC	1 cut(s)	1769
EaeI	YGGCCR	2 cut(s)	708, 1273
Eam1104I	CTCTTC	3 cut(s)	1042, 1539, 1976
EarI	CTCTTC	3 cut(s)	1042, 1539, 1976
EciI	GGCGGA	2 cut(s)	646, 1772
Eco130I	CCWWGG	4 cut(s)	333, 750, 2033, 2310
Eco31I	GGTCTC	1 cut(s)	323
Eco47I	GGWCC	3 cut(s)	592, 613, 1430
Eco47III	AGCGCT	1 cut(s)	117
Eco81I	CCTNAGG	1 cut(s)	957
Eco88I	CYCGRG	1 cut(s)	1203
Eco91I	GGTNACC	5 cut(s)	322, 1073, 1132, 2038, 2368
EcoNI	CCTNNNNNAGG	2 cut(s)	1502, 1757
EcoO109I	RGGNCCY	3 cut(s)	929, 1430, 1921
EcoO65I	GGTNACC	5 cut(s)	322, 1073, 1132, 2038, 2368
EcoRII	CCWGG	5 cut(s)	97, 492, 683, 1483, 1752
EcoT14I	CCWWGG	4 cut(s)	333, 750, 2033, 2310
EcoT22I	ATGCAT	1 cut(s)	2096
ErhI	CCWWGG	4 cut(s)	333, 750, 2033, 2310
Esp3I	CGTCTC	1 cut(s)	142
FaqI	GGGAC	3 cut(s)	519, 1120, 1292
FauI	CCCGC	1 cut(s)	2005
FauNDI	CATATG	1 cut(s)	1745
FblI	GTMKAC	1 cut(s)	2120
Fnu4HI	GCNGC	2 cut(s)	1951, 1954
FokI	GGATG	7 cut(s)	1918, 2016, 2032, 2156, 2213, 2228, 2399
Fsp4HI	GCNGC	2 cut(s)	1951, 1954
FspBI	CTAG	1 cut(s)	2451
GlaI	GCGC	4 cut(s)	6, 117, 1462, 1890
GluI	GCNGC	2 cut(s)	1951, 1954
GsaI	CCCAGC	2 cut(s)	126, 411
GsuI	CTGGAG	4 cut(s)	476, 710, 894, 2202
HaeII	RGCGCY	1 cut(s)	119
HaeIII	GGCC	9 cut(s)	347, 549, 710, 743, 931, 1275, 1353, 1923, 2081
HapII	CCGG	4 cut(s)	590, 1179, 1204, 1276
HgaI	GACGC	4 cut(s)	553, 1261, 1497, 1678
HhaI	GCGC	4 cut(s)	7, 118, 1463, 1891
Hin1I	GRCGYC	2 cut(s)	564, 1508
Hin6I	GCGC	4 cut(s)	5, 116, 1461, 1889
HinP1I	GCGC	4 cut(s)	5, 116, 1461, 1889
HincII	GTYRAC	1 cut(s)	1054
HindII	GTYRAC	1 cut(s)	1054
HpaII	CCGG	4 cut(s)	590, 1179, 1204, 1276
Hpy166II	GTNNAC	8 cut(s)	355, 808, 969, 1054, 1552, 1597, 1624, 2121
Hpy188I	TCNGA	3 cut(s)	243, 634, 641
Hpy188III	TCNNGA	7 cut(s)	289, 527, 727, 1387, 2068, 2162, 2492
Hpy8I	GTNNAC	8 cut(s)	355, 808, 969, 1054, 1552, 1597, 1624, 2121
Hpy99I	CGWCG	4 cut(s)	569, 1732, 2138, 2141
HpyAV	CCTTC	8 cut(s)	142, 217, 313, 409, 982, 1375, 1674, 2452
HpyCH4III	ACNGT	9 cut(s)	36, 555, 1162, 1271, 1696, 1994, 2050, 2111, 2380
HpyCH4IV	ACGT	5 cut(s)	403, 532, 784, 1304, 2057
HpyF10VI	GCNNNNNNNGC	2 cut(s)	656, 1422
HpyF3I	CTNAG	5 cut(s)	167, 240, 422, 957, 1434
HpySE526I	ACGT	5 cut(s)	403, 532, 784, 1304, 2057
Hsp92I	GRCGYC	2 cut(s)	564, 1508
HspAI	GCGC	4 cut(s)	5, 116, 1461, 1889
KroI	GCCGGC	1 cut(s)	1178
KroNI	GCCGGC	1 cut(s)	1180
Kzo9I	GATC	4 cut(s)	523, 891, 911, 1915
LguI	GCTCTTC	1 cut(s)	1976
LmnI	GCTCC	4 cut(s)	686, 944, 1093, 1413
LweI	GCATC	7 cut(s)	180, 892, 1591, 1722, 1940, 2103, 2233
MaeI	CTAG	1 cut(s)	2451
MaeII	ACGT	5 cut(s)	403, 532, 784, 1304, 2057
MalI	GATC	4 cut(s)	525, 893, 913, 1917
MboI	GATC	4 cut(s)	523, 891, 911, 1915
MflI	RGATCY	1 cut(s)	911
MhlI	GDGCHC	4 cut(s)	683, 1169, 1599, 1834
MlsI	TGGCCA	1 cut(s)	710
MluNI	TGGCCA	1 cut(s)	710
MlyI	GAGTC	5 cut(s)	856, 1059, 1458, 1779, 2390
MmeI	TCCRAC	3 cut(s)	619, 1541, 1959
Mox20I	TGGCCA	1 cut(s)	710
Mph1103I	ATGCAT	1 cut(s)	2096
MroNI	GCCGGC	1 cut(s)	1178
MroXI	GAANNNNTTC	1 cut(s)	2289
MscI	TGGCCA	1 cut(s)	710
MseI	TTAA	7 cut(s)	17, 198, 390, 981, 1856, 2061, 2465
MslI	CAYNNNNRTG	1 cut(s)	1712
Msp20I	TGGCCA	1 cut(s)	710
MspI	CCGG	4 cut(s)	590, 1179, 1204, 1276
MspR9I	CCNGG	9 cut(s)	99, 494, 590, 685, 1204, 1205, 1277, 1485, 1754
MvaI	CCWGG	5 cut(s)	99, 494, 685, 1485, 1754
MvnI	CGCG	2 cut(s)	611, 1891
MwoI	GCNNNNNNNGC	2 cut(s)	656, 1422
NaeI	GCCGGC	1 cut(s)	1180
NciI	CCSGG	4 cut(s)	590, 1204, 1205, 1277
NcoI	CCATGG	2 cut(s)	333, 2310
NdeI	CATATG	1 cut(s)	1745
NdeII	GATC	4 cut(s)	523, 891, 911, 1915
NgoMIV	GCCGGC	1 cut(s)	1178
NlaIV	GGNNCC	3 cut(s)	913, 946, 1922
NmuCI	GTSAC	4 cut(s)	1073, 1197, 1690, 2368
NsiI	ATGCAT	1 cut(s)	2096
NspI	RCATGY	3 cut(s)	979, 1461, 2094
OliI	CACNNNNGTG	1 cut(s)	1712
PaeI	GCATGC	1 cut(s)	1461
PagI	TCATGA	2 cut(s)	2067, 2491
PciSI	GCTCTTC	1 cut(s)	1976
PcsI	WCGNNNNNNNCGW	2 cut(s)	1646, 2103
PdiI	GCCGGC	1 cut(s)	1180
PdmI	GAANNNNTTC	1 cut(s)	2289
PfeI	GAWTC	7 cut(s)	128, 285, 603, 688, 1012, 1084, 2495
PflMI	CCANNNNNTGG	2 cut(s)	104, 1558
PfoI	TCCNGGA	3 cut(s)	492, 588, 683
PkrI	GCNGC	2 cut(s)	1952, 1955
PleI	GAGTC	5 cut(s)	855, 1058, 1457, 1778, 2390
PpsI	GAGTC	5 cut(s)	855, 1058, 1457, 1778, 2390
Ppu21I	YACGTR	1 cut(s)	404
PpuMI	RGGWCCY	1 cut(s)	1430
PsiI	TTATAA	1 cut(s)	161
Psp1406I	AACGTT	2 cut(s)	1304, 2057
Psp5II	RGGWCCY	1 cut(s)	1430
Psp6I	CCWGG	5 cut(s)	97, 492, 683, 1483, 1752
PspEI	GGTNACC	5 cut(s)	322, 1073, 1132, 2038, 2368
PspFI	CCCAGC	2 cut(s)	122, 407
PspGI	CCWGG	5 cut(s)	97, 492, 683, 1483, 1752
PspN4I	GGNNCC	3 cut(s)	913, 946, 1922
PspPI	GGNCC	8 cut(s)	346, 548, 592, 613, 929, 1352, 1430, 1921
PspPPI	RGGWCCY	1 cut(s)	1430
PsuI	RGATCY	1 cut(s)	911
RsaI	GTAC	9 cut(s)	268, 313, 402, 783, 1227, 1793, 2179, 2382, 2455
RsaNI	GTAC	9 cut(s)	267, 312, 401, 782, 1226, 1792, 2178, 2381, 2454
RseI	CAYNNNNRTG	1 cut(s)	1712
SapI	GCTCTTC	1 cut(s)	1976
SaqAI	TTAA	7 cut(s)	17, 198, 390, 981, 1856, 2061, 2465
SatI	GCNGC	2 cut(s)	1951, 1954
Sau3AI	GATC	4 cut(s)	523, 891, 911, 1915
Sau96I	GGNCC	8 cut(s)	346, 548, 592, 613, 929, 1352, 1430, 1921
ScaI	AGTACT	2 cut(s)	1227, 2179
SchI	GAGTC	5 cut(s)	856, 1059, 1458, 1779, 2390
ScrFI	CCNGG	9 cut(s)	99, 494, 590, 685, 1204, 1205, 1277, 1485, 1754
SduI	GDGCHC	4 cut(s)	683, 1169, 1599, 1834
SfaNI	GCATC	7 cut(s)	180, 892, 1591, 1722, 1940, 2103, 2233
SinI	GGWCC	3 cut(s)	592, 613, 1430
SmaI	CCCGGG	1 cut(s)	1205
SmiMI	CAYNNNNRTG	1 cut(s)	1712
SmlI	CTYRAG	2 cut(s)	1502, 2162
SmoI	CTYRAG	2 cut(s)	1502, 2162
SphI	GCATGC	1 cut(s)	1461
SsiI	CCGC	9 cut(s)	611, 657, 1346, 1712, 1757, 1891, 1951, 1954, 2012
SspI	AATATT	1 cut(s)	1812
SspMI	CTAG	1 cut(s)	2451
StyD4I	CCNGG	9 cut(s)	97, 492, 588, 683, 1202, 1203, 1275, 1483, 1752
StyI	CCWWGG	4 cut(s)	333, 750, 2033, 2310
TaaI	ACNGT	9 cut(s)	36, 555, 1162, 1271, 1696, 1994, 2050, 2111, 2380
TaiI	ACGT	5 cut(s)	406, 535, 787, 1307, 2060
TaqI	TCGA	7 cut(s)	478, 1447, 1727, 1768, 1944, 2097, 2459
TaqII	GACCGA	1 cut(s)	1106
TatI	WGTACW	3 cut(s)	1225, 2177, 2453
TauI	GCSGC	2 cut(s)	1953, 1956
TfiI	GAWTC	7 cut(s)	128, 285, 603, 688, 1012, 1084, 2495
Tru1I	TTAA	7 cut(s)	17, 198, 390, 981, 1856, 2061, 2465
Tru9I	TTAA	7 cut(s)	17, 198, 390, 981, 1856, 2061, 2465
TscAI	CASTG	6 cut(s)	442, 558, 744, 1276, 1387, 2116
TseFI	GTSAC	4 cut(s)	1073, 1197, 1690, 2368
Tsp45I	GTSAC	4 cut(s)	1073, 1197, 1690, 2368
TspDTI	ATGAA	7 cut(s)	1247, 1526, 1854, 2186, 2252, 2323, 2487
TspGWI	ACGGA	4 cut(s)	534, 1638, 1833, 1888
TspMI	CCCGGG	1 cut(s)	1203
TspRI	CASTG	6 cut(s)	442, 558, 744, 1276, 1387, 2116
Van91I	CCANNNNNTGG	2 cut(s)	104, 1558
VneI	GTGCAC	1 cut(s)	1595
VpaK11BI	GGWCC	3 cut(s)	592, 613, 1430
XagI	CCTNNNNNAGG	2 cut(s)	1502, 1757
XapI	RAATTY	6 cut(s)	13, 200, 997, 2255, 2289, 2483
XceI	RCATGY	3 cut(s)	979, 1461, 2094
XcmI	CCANNNNNNNNNTGG	4 cut(s)	105, 340, 795, 2380
XmaI	CCCGGG	1 cut(s)	1203
XmiI	GTMKAC	1 cut(s)	2120
XmnI	GAANNNNTTC	1 cut(s)	2289
XspI	CTAG	1 cut(s)	2451
ZrmI	AGTACT	2 cut(s)	1227, 2179
Zsp2I	ATGCAT	1 cut(s)	2096

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.