Rw1G002660

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001291
Physical Location & Seq
Reverse (-)
4778268 .. 4781227
2960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G002660.1

Sequence Viewer

Length: 2514 bp
ATGGCCAAAACGACATTCAATATCACGTATGGAAACAAAACCTCTTTCAATGTGTGTTATAACAAAACAGAGACCCGAAACACCGGGCTATGGAGTGCAGAAAACCCCTTGGTATCATCTCTCCCAATGCTCATCATCCAACTGCTCGCTGTCTTGTTGCTCGGTCACATTCTTGCCTACCTCCTCAAACCCTTGCGCCAACCACGCATTGTTTCTAATATTCTTGCTGGCGTTCTATTAGGTCCATCTTTCTTTGCTATCACGAGAATCGTGGGAGTTTCCATACTTCCTTTCGATAGCATAATGACCTTGGAGACTCTGGCAAACTTGGGGCTCGTGTACCACATTTTCCTCATTGGGTTAGAGTTTGAATTCAGACCAGTAGTACGTGCAGGGACAAAGGCTCTCAGCATTGCCCTCGCCGGCTTGGTCTTTGCCATCCCCGCCGGGTGGGCATTGCATACCTATGTACTCTTCCGCGACTTTGAAACCGTGAAAGCCAAAAGCAATATGAAAAATGTAGCGTACGGCCCTATCTTCTGGGGCATTGTCCTCTCCAGCACCAACTTCGCTGATCTCACCCAAATTCTTGCCGACTTCAAGCTTCTCCACTCGGAGGTGGGACGACTAGCCTTATCCGGTGCCATTATCTCCGATGTGGCGTCATGGTTTCTTTTTCTTGTGGTAATGGCCATACTCGGTTATGGTAAATTGTACACGGTGTTGTTAACGTTAGGCTTCATTGCATACAGTATTTTTTTACTTCGGCCAGCTCTCGCCTGGGTAATGCGCCACACCACCAAGGAAGACGGCTATAGTGATGTCCAAATATGCTTCATTCTGTTTGGGATTGTATTGAGTGGATTAATAACCGATGCCTGCGGTGGACACTCCATCGTCGGACCTTTCATGTTGGGAGCAATTATGCCCAGGGGGGTACACTTCAAACAAATGTTTGCAGATCGTGTTGGAAAATTTGTGGAAAGCCTGATGATGCCCCTCTTTTACTTGGTCATTGGGGCGAGATTCCATTCTTTTTGGCTACTCGGTGATCTAAATAACAATCTGCAAGTTGAATCTACCGTGCCTCAGCTTGTTGGGGTGATCATCCTGGCTTTCACAGCTAAGATTGTCGGCACTTTCATCGCTGCTGTACTCAACAAAATGACACCTCGCGATAGTTTAGTTCTGGGAATCCTCATGAACACTAAAGGCTTATTGGCACTCCTTATACTCACCTCCGCTCGTGACCTAAAGGCTTTGGACACCCAAACATATGGAGTAATGTTGGTTGCAATTTGGGCAATGACGGTTCCCGTTGGACCCTTCCTAGCCCTCATTTGCAAGTCCACCAAGAGTTCTACACAATATAAACAGAGAAGCATACAAAGTGTAGGAGTTGAAACAGAGCTTCGAATCCTTGCATGCACCCATACTGCGAGCAACGCATCAGGCATCATAAGCCTCATTGACGCTTCCAATCCATCCAGTAAATCCCCAATTCATGTCTTTGCTGTCAAACTGGTGGAACTTACCGACCATAATGCTGCTATGCTCATCGTGCATGATTCTTGCAACAAAACTGGAGCAGATGGCAACGACGACTTGAGCTCCAATGCATTTCAGAACTATGCAAGGCAAAGAGAAAATGTCTCCGTTCAATCACTCACCGCAGTGTCTGCCTATGCCACTATGCACGAGGATATATGCAACATGGCTGACGAGAAGCACGTCAACTTCATAATCCTCCCGTTCCGTATGCCAACTACTATTGATGGAGTCCCAGATGATCGTTATGATCCTCTTCTCACGGAAATTAGTAAGAAGGTCATGGAAAATGCAAAGTGCTCTGTAGGTCTTTTGGTGGATTGTGGGCTTGTCACATTCCACAACGCTTCTTTGGAGTCCCAAAACTTCGTCATGTTTTTCATTGGAGGGCCAGATGATCGAGAGGCATTAGCGTACGCATGGAGGATGGCTAGGCATCCTAGGGTGAACTTAAAAGTTATACGGTTCACCCTCAGTAAAGAGGCATGGGAGGTGAGTTATCATCACGATGGTGATGAGGAATACAAGACAAATGAAAACATTAGGCAGCAGAAACAACTAGACGACGCATGCATAGATAAGTTCAAACTTGAGTCCATGAATGAACCCTCCTTAGTTCTATGTGAGAAGCTGGTGAATAATTTGGAAGACATTATCGATATAGTAAGCACCATGGAAGGTCAATATCAGCTGTACATAGTGGGAAGAGGTCATGATGGTTGCTCCCCAATAACAGCACCGTTGTTCGAATGCAACAAGACCAATAAGCTCGGAACTTGGGGGGAAACATTGGCATCTTCAAGCGTCATGGGAAGTACATCCCTTCTTATAATACAGCAGGGTGCCTCTGTGGATGAACTAGATGAGACAATGGGATCTGACGGGAAACATTTTGGGGATACAACTCGGCAACCTCCCGTAAAAGACGCCGAAGATTCCTTGCTTAGAACTTCATCAGTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

837

Amino Acids

92.06

Weight (kDa)

6.15

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 55 - 442 2.2e-49 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 503 - 628 4.2e-09 Plant cation/H(+) antiporter domain
CHX17_C PF23259 641 - 796 8.6e-10 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 60, 2378
AccB1I GGYRCC 2 cut(s) 641, 2390
AccBSI CCGCTC 1 cut(s) 1244
AccII CGCG 2 cut(s) 480, 1176
AciI CCGC 5 cut(s) 444, 478, 882, 1242, 1671
AclI AACGTT 1 cut(s) 731
AclWI GGATC 2 cut(s) 1793, 2431
AcoI YGGCCR 3 cut(s) 3, 690, 767
AcsI RAATTY 3 cut(s) 371, 585, 974
AcyI GRCGYC 2 cut(s) 662, 2475
AfiI CCNNNNNNNGG 3 cut(s) 90, 450, 616
AjiI CACGTC 1 cut(s) 1732
AjnI CCWGG 3 cut(s) 779, 929, 1110
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AleI CACNNNNGTG 2 cut(s) 1673, 2058
AluBI AGCT 9 cut(s) 604, 773, 1093, 1124, 1411, 1611, 2179, 2239, 2317
AluI AGCT 9 cut(s) 604, 773, 1093, 1124, 1411, 1611, 2179, 2239, 2317
Alw21I GWGCWC 2 cut(s) 1613, 1850
Alw26I GTCTC 4 cut(s) 65, 308, 1657, 2408
AlwI GGATC 2 cut(s) 1793, 2431
AlwNI CAGNNNCTG 1 cut(s) 1679
AoxI GGCC 5 cut(s) 3, 529, 690, 767, 1937
ApeKI GCWGC 3 cut(s) 1148, 1547, 2095
ApoI RAATTY 3 cut(s) 371, 585, 974
AseI ATTAAT 1 cut(s) 866
AspA2I CCTAGG 1 cut(s) 1988
AspLEI GCGC 2 cut(s) 198, 792
AspS9I GGNCC 5 cut(s) 242, 530, 902, 1322, 1937
AsuC2I CCSGG 2 cut(s) 85, 448
AsuII TTCGAA 2 cut(s) 1414, 2295
AvaII GGWCC 3 cut(s) 242, 902, 1322
AvrII CCTAGG 1 cut(s) 1988
BalI TGGCCA 2 cut(s) 5, 692
BanI GGYRCC 2 cut(s) 641, 2390
BanII GRGCYC 2 cut(s) 336, 1613
BauI CACGAG 4 cut(s) 262, 335, 1245, 1697
BbsI GAAGAC 2 cut(s) 813, 2202
Bbv12I GWGCWC 2 cut(s) 1613, 1850
BbvCI CCTCAGC 1 cut(s) 1089
BbvI GCAGC 3 cut(s) 1135, 1534, 2107
BccI CCATC 9 cut(s) 253, 446, 902, 1492, 1586, 1769, 1969, 2051, 2258
BceAI ACGGC 2 cut(s) 544, 826
BcgI CGANNNNNNTGC 8 cut(s) 400, 434, 550, 584, 1126, 1160, 1526, 1560
BciT130I CCWGG 3 cut(s) 781, 931, 1112
BciVI GTATCC 1 cut(s) 2440
BclI TGATCA 1 cut(s) 1104
BcnI CCSGG 2 cut(s) 85, 448
BcoDI GTCTC 4 cut(s) 65, 308, 1657, 2408
BfaI CTAG 7 cut(s) 629, 1331, 1980, 1989, 2108, 2408, 2512
BfmI CTRYAG 2 cut(s) 814, 1851
BfuI GTATCC 1 cut(s) 2440
BglI GCCNNNNNGGC 1 cut(s) 452
BisI GCNGC 3 cut(s) 1149, 1548, 2096
BlnI CCTAGG 1 cut(s) 1988
BlsI GCNGC 3 cut(s) 1150, 1549, 2097
Bme1390I CCNGG 5 cut(s) 85, 448, 781, 931, 1112
Bme18I GGWCC 3 cut(s) 242, 902, 1322
BmgBI CACGTC 1 cut(s) 1732
BmgT120I GGNCC 5 cut(s) 242, 530, 902, 1322, 1937
BmiI GGNNCC 4 cut(s) 643, 1314, 1324, 2392
BmrFI CCNGG 5 cut(s) 85, 448, 781, 931, 1112
BmsI GCATC 6 cut(s) 865, 984, 1457, 1464, 1993, 2351
BpiI GAAGAC 2 cut(s) 813, 2202
BpmI CTGGAG 2 cut(s) 541, 1605
Bpu10I CCTNAGC 1 cut(s) 1089
Bpu14I TTCGAA 2 cut(s) 1414, 2295
BpuEI CTTGAG 2 cut(s) 1627, 2159
BpuMI CCSGG 2 cut(s) 85, 448
Bsa29I ATCGAT 1 cut(s) 2205
BsaAI YACGTR 2 cut(s) 27, 389
BsaHI GRCGYC 2 cut(s) 662, 2475
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 8 cut(s) 108, 309, 780, 801, 929, 930, 1988, 2220
BsaWI WCCGGW 1 cut(s) 638
BsaXI ACNNNNNCTCC 2 cut(s) 1595, 1625
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 450, 616
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 4 cut(s) 380, 1488, 1527, 1588
Bse3DI GCAATG 4 cut(s) 411, 455, 741, 1311
BseBI CCWGG 3 cut(s) 781, 931, 1112
BseCI ATCGAT 1 cut(s) 2205
BseDI CCNNGG 8 cut(s) 108, 309, 780, 801, 929, 930, 1988, 2220
BseGI GGATG 8 cut(s) 135, 438, 1107, 1484, 1980, 1984, 2366, 2407
BseLI CCNNNNNNNGG 3 cut(s) 90, 450, 616
BseMI GCAATG 4 cut(s) 411, 455, 741, 1311
BseMII CTCAG 3 cut(s) 421, 1103, 2035
BseNI ACTGG 4 cut(s) 380, 1488, 1527, 1588
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 3 cut(s) 1135, 1534, 2107
BsgI GTGCAG 2 cut(s) 117, 411
Bsh1236I CGCG 2 cut(s) 480, 1176
BshFI GGCC 5 cut(s) 5, 531, 692, 769, 1939
BshNI GGYRCC 2 cut(s) 641, 2390
BshVI ATCGAT 1 cut(s) 2205
BsiHKAI GWGCWC 2 cut(s) 1613, 1850
BsiSI CCGG 4 cut(s) 84, 423, 447, 639
BsiWI CGTACG 2 cut(s) 525, 1962
BslFI GGGAC 4 cut(s) 409, 636, 1766, 1891
BslI CCNNNNNNNGG 3 cut(s) 90, 450, 616
BsmAI GTCTC 4 cut(s) 65, 308, 1657, 2408
BsmFI GGGAC 4 cut(s) 409, 636, 1766, 1891
BsmI GAATGC 1 cut(s) 2303
BsnI GGCC 5 cut(s) 5, 531, 692, 769, 1939
Bso31I GGTCTC 1 cut(s) 65
Bsp119I TTCGAA 2 cut(s) 1414, 2295
Bsp1286I GDGCHC 3 cut(s) 336, 1613, 1850
Bsp1407I TGTACA 2 cut(s) 714, 2241
Bsp143I GATC 8 cut(s) 574, 961, 1051, 1104, 1789, 1798, 1945, 2423
Bsp19I CCATGG 1 cut(s) 2220
Bsp68I TCGCGA 1 cut(s) 1176
BspACI CCGC 5 cut(s) 444, 478, 882, 1242, 1671
BspANI GGCC 5 cut(s) 5, 531, 692, 769, 1939
BspCNI CTCAG 3 cut(s) 420, 1102, 2034
BspDI ATCGAT 1 cut(s) 2205
BspFNI CGCG 2 cut(s) 480, 1176
BspHI TCATGA 2 cut(s) 1200, 2260
BspLI GGNNCC 4 cut(s) 643, 1314, 1324, 2392
BspPI GGATC 2 cut(s) 1793, 2431
BspT104I TTCGAA 2 cut(s) 1414, 2295
BspT107I GGYRCC 2 cut(s) 641, 2390
BspTNI GGTCTC 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 1244
BsrDI GCAATG 4 cut(s) 411, 455, 741, 1311
BsrFI RCCGGY 1 cut(s) 422
BsrGI TGTACA 2 cut(s) 714, 2241
BsrI ACTGG 4 cut(s) 380, 1488, 1527, 1588
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 8 cut(s) 108, 309, 780, 801, 929, 930, 1988, 2220
BssMI GATC 8 cut(s) 574, 961, 1051, 1104, 1789, 1798, 1945, 2423
BssNI GRCGYC 2 cut(s) 662, 2475
BssSI CACGAG 4 cut(s) 262, 335, 1245, 1697
BssT1I CCWWGG 5 cut(s) 108, 309, 801, 1988, 2220
Bst2BI CACGAG 4 cut(s) 262, 335, 1245, 1697
Bst2UI CCWGG 3 cut(s) 781, 931, 1112
Bst4CI ACNGT 7 cut(s) 493, 721, 752, 1084, 1312, 2013, 2289
Bst6I CTCTTC 3 cut(s) 479, 1809, 2248
BstACI GRCGYC 2 cut(s) 662, 2475
BstAPI GCANNNNNTGC 1 cut(s) 1679
BstAUI TGTACA 2 cut(s) 714, 2241
BstBAI YACGTR 2 cut(s) 27, 389
BstBI TTCGAA 2 cut(s) 1414, 2295
BstC8I GCNNGC 8 cut(s) 147, 229, 424, 771, 880, 1426, 1441, 2119
BstDEI CTNAG 6 cut(s) 407, 1089, 1125, 2021, 2161, 2492
BstDSI CCRYGG 1 cut(s) 2220
BstF5I GGATG 8 cut(s) 135, 438, 1107, 1484, 1980, 1984, 2366, 2407
BstFNI CGCG 2 cut(s) 480, 1176
BstHHI GCGC 2 cut(s) 198, 792
BstKTI GATC 8 cut(s) 577, 964, 1054, 1107, 1792, 1801, 1948, 2426
BstMAI GTCTC 4 cut(s) 65, 308, 1657, 2408
BstMBI GATC 8 cut(s) 574, 961, 1051, 1104, 1789, 1798, 1945, 2423
BstMWI GCNNNNNNNGC 9 cut(s) 204, 443, 452, 1121, 1301, 1445, 1461, 1561, 1679
BstNI CCWGG 3 cut(s) 781, 931, 1112
BstNSI RCATGY 2 cut(s) 1428, 2121
BstSCI CCNGG 5 cut(s) 83, 446, 779, 929, 1110
BstSFI CTRYAG 2 cut(s) 814, 1851
BstUI CGCG 2 cut(s) 480, 1176
BstV1I GCAGC 3 cut(s) 1135, 1534, 2107
BstV2I GAAGAC 2 cut(s) 813, 2202
BstX2I RGATCY 1 cut(s) 2423
BstXI CCANNNNNNTGG 1 cut(s) 1277
BstYI RGATCY 1 cut(s) 2423
Bsu15I ATCGAT 1 cut(s) 2205
BsuI GTATCC 1 cut(s) 2440
BsuRI GGCC 5 cut(s) 5, 531, 692, 769, 1939
BsuTUI ATCGAT 1 cut(s) 2205
BtgI CCRYGG 1 cut(s) 2220
BtgZI GCGATG 1 cut(s) 1129
BtrI CACGTC 1 cut(s) 1732
BtsCI GGATG 8 cut(s) 135, 438, 1107, 1484, 1980, 1984, 2366, 2407
BtsI GCAGTG 1 cut(s) 1680
BtsIMutI CAGTG 1 cut(s) 1680
BtuMI TCGCGA 1 cut(s) 1176
Cac8I GCNNGC 8 cut(s) 147, 229, 424, 771, 880, 1426, 1441, 2119
CaiI CAGNNNCTG 1 cut(s) 1679
CciI TCATGA 2 cut(s) 1200, 2260
CfoI GCGC 2 cut(s) 198, 792
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 5 cut(s) 242, 530, 902, 1322, 1937
ClaI ATCGAT 1 cut(s) 2205
CseI GACGC 5 cut(s) 651, 1481, 2123, 2341, 2483
DdeI CTNAG 6 cut(s) 407, 1089, 1125, 2021, 2161, 2492
DpnI GATC 8 cut(s) 576, 963, 1053, 1106, 1791, 1800, 1947, 2425
DpnII GATC 8 cut(s) 574, 961, 1051, 1104, 1789, 1798, 1945, 2423
EaeI YGGCCR 3 cut(s) 3, 690, 767
Eam1104I CTCTTC 3 cut(s) 479, 1809, 2248
EarI CTCTTC 3 cut(s) 479, 1809, 2248
Ecl136II GAGCTC 1 cut(s) 1611
Eco130I CCWWGG 5 cut(s) 108, 309, 801, 1988, 2220
Eco24I GRGCYC 2 cut(s) 336, 1613
Eco31I GGTCTC 1 cut(s) 65
Eco47I GGWCC 3 cut(s) 242, 902, 1322
Eco53kI GAGCTC 1 cut(s) 1611
EcoICRI GAGCTC 1 cut(s) 1611
EcoRI GAATTC 1 cut(s) 371
EcoRII CCWGG 3 cut(s) 779, 929, 1110
EcoT14I CCWWGG 5 cut(s) 108, 309, 801, 1988, 2220
EcoT22I ATGCAT 2 cut(s) 1621, 2123
EcoT38I GRGCYC 2 cut(s) 336, 1613
ErhI CCWWGG 5 cut(s) 108, 309, 801, 1988, 2220
FaqI GGGAC 4 cut(s) 409, 636, 1766, 1891
FauI CCCGC 1 cut(s) 451
FauNDI CATATG 1 cut(s) 1276
FbaI TGATCA 1 cut(s) 1104
Fnu4HI GCNGC 3 cut(s) 1149, 1548, 2096
FokI GGATG 8 cut(s) 122, 425, 1094, 1471, 1971, 1987, 2353, 2414
FriOI GRGCYC 2 cut(s) 336, 1613
Fsp4HI GCNGC 3 cut(s) 1149, 1548, 2096
FspBI CTAG 7 cut(s) 629, 1331, 1980, 1989, 2108, 2408, 2512
GlaI GCGC 2 cut(s) 197, 791
GluI GCNGC 3 cut(s) 1149, 1548, 2096
GsuI CTGGAG 2 cut(s) 541, 1605
HaeIII GGCC 5 cut(s) 5, 531, 692, 769, 1939
HapII CCGG 4 cut(s) 84, 423, 447, 639
HgaI GACGC 5 cut(s) 651, 1481, 2123, 2341, 2483
HhaI GCGC 2 cut(s) 198, 792
Hin1I GRCGYC 2 cut(s) 662, 2475
Hin6I GCGC 2 cut(s) 196, 790
HinP1I GCGC 2 cut(s) 196, 790
HincII GTYRAC 2 cut(s) 729, 1735
HindII GTYRAC 2 cut(s) 729, 1735
HindIII AAGCTT 1 cut(s) 602
HpaI GTTAAC 1 cut(s) 729
HpaII CCGG 4 cut(s) 84, 423, 447, 639
Hpy188I TCNGA 7 cut(s) 377, 616, 655, 902, 1626, 2321, 2428
Hpy188III TCNNGA 7 cut(s) 262, 1175, 1201, 1247, 1949, 2054, 2261
Hpy99I CGWCG 3 cut(s) 902, 1604, 2117
HpyAV CCTTC 4 cut(s) 1336, 1819, 2219, 2381
HpyCH4III ACNGT 7 cut(s) 493, 721, 752, 1084, 1312, 2013, 2289
HpyCH4IV ACGT 4 cut(s) 26, 388, 731, 1731
HpyF10VI GCNNNNNNNGC 9 cut(s) 204, 443, 452, 1121, 1301, 1445, 1461, 1561, 1679
HpyF3I CTNAG 6 cut(s) 407, 1089, 1125, 2021, 2161, 2492
HpySE526I ACGT 4 cut(s) 26, 388, 731, 1731
Hsp92I GRCGYC 2 cut(s) 662, 2475
HspAI GCGC 2 cut(s) 196, 790
KroI GCCGGC 1 cut(s) 422
KroNI GCCGGC 1 cut(s) 424
Ksp22I TGATCA 1 cut(s) 1104
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 8 cut(s) 574, 961, 1051, 1104, 1789, 1798, 1945, 2423
LmnI GCTCC 4 cut(s) 917, 1586, 1616, 2276
Lsp1109I GCAGC 3 cut(s) 1135, 1534, 2107
LweI GCATC 6 cut(s) 865, 984, 1457, 1464, 1993, 2351
MaeI CTAG 7 cut(s) 629, 1331, 1980, 1989, 2108, 2408, 2512
MaeII ACGT 4 cut(s) 26, 388, 731, 1731
MaeIII GTNAC 3 cut(s) 164, 1247, 1879
MalI GATC 8 cut(s) 576, 963, 1053, 1106, 1791, 1800, 1947, 2425
MbiI CCGCTC 1 cut(s) 1244
MboI GATC 8 cut(s) 574, 961, 1051, 1104, 1789, 1798, 1945, 2423
MboII GAAGA 8 cut(s) 466, 529, 818, 1796, 2207, 2265, 2337, 2492
MflI RGATCY 1 cut(s) 2423
MhlI GDGCHC 3 cut(s) 336, 1613, 1850
MlsI TGGCCA 2 cut(s) 5, 692
MluCI AATT 9 cut(s) 371, 585, 710, 921, 974, 1296, 1500, 1815, 2188
MluNI TGGCCA 2 cut(s) 5, 692
MlyI GAGTC 4 cut(s) 310, 1788, 1913, 2150
MmeI TCCRAC 4 cut(s) 163, 880, 949, 1300
Mox20I TGGCCA 2 cut(s) 5, 692
Mph1103I ATGCAT 2 cut(s) 1621, 2123
MroNI GCCGGC 1 cut(s) 422
MscI TGGCCA 2 cut(s) 5, 692
MseI TTAA 3 cut(s) 728, 866, 2000
MslI CAYNNNNRTG 4 cut(s) 465, 1673, 2055, 2058
Msp20I TGGCCA 2 cut(s) 5, 692
MspA1I CMGCKG 1 cut(s) 2239
MspI CCGG 4 cut(s) 84, 423, 447, 639
MspR9I CCNGG 5 cut(s) 85, 448, 781, 931, 1112
Mva1269I GAATGC 1 cut(s) 2303
MvaI CCWGG 3 cut(s) 781, 931, 1112
MvnI CGCG 2 cut(s) 480, 1176
MwoI GCNNNNNNNGC 9 cut(s) 204, 443, 452, 1121, 1301, 1445, 1461, 1561, 1679
NaeI GCCGGC 1 cut(s) 424
NciI CCSGG 2 cut(s) 85, 448
NcoI CCATGG 1 cut(s) 2220
NdeI CATATG 1 cut(s) 1276
NdeII GATC 8 cut(s) 574, 961, 1051, 1104, 1789, 1798, 1945, 2423
NgoMIV GCCGGC 1 cut(s) 422
NlaIV GGNNCC 4 cut(s) 643, 1314, 1324, 2392
NmeAIII GCCGAG 1 cut(s) 2434
NmuCI GTSAC 3 cut(s) 164, 1247, 1879
NruI TCGCGA 1 cut(s) 1176
NsiI ATGCAT 2 cut(s) 1621, 2123
NspI RCATGY 2 cut(s) 1428, 2121
NspV TTCGAA 2 cut(s) 1414, 2295
OliI CACNNNNGTG 2 cut(s) 1673, 2058
PaeI GCATGC 2 cut(s) 1428, 2121
PagI TCATGA 2 cut(s) 1200, 2260
PasI CCCWGGG 1 cut(s) 930
PcsI WCGNNNNNNNCGW 1 cut(s) 1728
PctI GAATGC 1 cut(s) 2303
PdiI GCCGGC 1 cut(s) 424
PfeI GAWTC 7 cut(s) 267, 1026, 1076, 1194, 1416, 1568, 2483
Pfl23II CGTACG 2 cut(s) 525, 1962
PkrI GCNGC 3 cut(s) 1150, 1549, 2097
PleI GAGTC 4 cut(s) 310, 1787, 1912, 2149
PpsI GAGTC 4 cut(s) 310, 1787, 1912, 2149
Ppu21I YACGTR 2 cut(s) 27, 389
PshBI ATTAAT 1 cut(s) 866
PsiI TTATAA 2 cut(s) 60, 2378
Psp124BI GAGCTC 1 cut(s) 1613
Psp1406I AACGTT 1 cut(s) 731
Psp6I CCWGG 3 cut(s) 779, 929, 1110
PspGI CCWGG 3 cut(s) 779, 929, 1110
PspLI CGTACG 2 cut(s) 525, 1962
PspN4I GGNNCC 4 cut(s) 643, 1314, 1324, 2392
PspPI GGNCC 5 cut(s) 242, 530, 902, 1322, 1937
PstNI CAGNNNCTG 1 cut(s) 1679
PsuI RGATCY 1 cut(s) 2423
PvuII CAGCTG 1 cut(s) 2239
RruI TCGCGA 1 cut(s) 1176
RseI CAYNNNNRTG 4 cut(s) 465, 1673, 2055, 2058
SacI GAGCTC 1 cut(s) 1613
SaqAI TTAA 3 cut(s) 728, 866, 2000
SatI GCNGC 3 cut(s) 1149, 1548, 2096
Sau3AI GATC 8 cut(s) 574, 961, 1051, 1104, 1789, 1798, 1945, 2423
Sau96I GGNCC 5 cut(s) 242, 530, 902, 1322, 1937
SchI GAGTC 4 cut(s) 310, 1788, 1913, 2150
ScrFI CCNGG 5 cut(s) 85, 448, 781, 931, 1112
SduI GDGCHC 3 cut(s) 336, 1613, 1850
SfaNI GCATC 6 cut(s) 865, 984, 1457, 1464, 1993, 2351
SfcI CTRYAG 2 cut(s) 814, 1851
SfuI TTCGAA 2 cut(s) 1414, 2295
SinI GGWCC 3 cut(s) 242, 902, 1322
SmiMI CAYNNNNRTG 4 cut(s) 465, 1673, 2055, 2058
SmlI CTYRAG 2 cut(s) 1606, 2138
SmoI CTYRAG 2 cut(s) 1606, 2138
SphI GCATGC 2 cut(s) 1428, 2121
Sse9I AATT 9 cut(s) 371, 585, 710, 921, 974, 1296, 1500, 1815, 2188
SsiI CCGC 5 cut(s) 444, 478, 882, 1242, 1671
SspI AATATT 1 cut(s) 220
SspMI CTAG 7 cut(s) 629, 1331, 1980, 1989, 2108, 2408, 2512
SstI GAGCTC 1 cut(s) 1613
StyD4I CCNGG 5 cut(s) 83, 446, 779, 929, 1110
StyI CCWWGG 5 cut(s) 108, 309, 801, 1988, 2220
TaaI ACNGT 7 cut(s) 493, 721, 752, 1084, 1312, 2013, 2289
TaiI ACGT 4 cut(s) 29, 391, 734, 1734
TaqI TCGA 5 cut(s) 294, 1414, 1948, 2205, 2295
TaqII GACCGA 1 cut(s) 152
TasI AATT 9 cut(s) 371, 585, 710, 921, 974, 1296, 1500, 1815, 2188
TatI WGTACW 5 cut(s) 469, 714, 1153, 2241, 2363
TfiI GAWTC 7 cut(s) 267, 1026, 1076, 1194, 1416, 1568, 2483
Tru1I TTAA 3 cut(s) 728, 866, 2000
Tru9I TTAA 3 cut(s) 728, 866, 2000
TscAI CASTG 1 cut(s) 1680
TseFI GTSAC 3 cut(s) 164, 1247, 1879
TseI GCWGC 3 cut(s) 1148, 1547, 2095
Tsp45I GTSAC 3 cut(s) 164, 1247, 1879
TspGWI ACGGA 3 cut(s) 1645, 1745, 1826
TspRI CASTG 1 cut(s) 1680
VpaK11BI GGWCC 3 cut(s) 242, 902, 1322
VspI ATTAAT 1 cut(s) 866
XapI RAATTY 3 cut(s) 371, 585, 974
XceI RCATGY 2 cut(s) 1428, 2121
XcmI CCANNNNNNNNNTGG 1 cut(s) 777
XmaJI CCTAGG 1 cut(s) 1988
XspI CTAG 7 cut(s) 629, 1331, 1980, 1989, 2108, 2408, 2512
Zsp2I ATGCAT 2 cut(s) 1621, 2123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.