Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001291

Physical Location & Seq

Genomic Coordinates Reverse (-)

10233020 .. 10236685

Gene Structure 3666 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1139600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2598 bp

ATGGCTGCTCGGGGTCAACGACGGGGCAATGGTTGGGTAGTACTGGAGGCGAATGTAGAAACTATAAGGGTGTGTTTTAATAAAACTTTGACACACACCACTGGCTTGTGGAAGGCTGAAAATGCCTTGCTTTCAACTCTTCCTCTCTTCTTCATCCAACAAGCTGTGATCATGTCCCTTTGTTACCTTCTCAAGCTGCTTCTTACACCTTTTCACATTCCTCGCGTTATTTCTCACATTATTGGTGGCGTGATATTGGGTCCATCTGCGTTTGGGGGCACGGCGTTCGCTCGCAACTACGTATTTCCATTCAAAAGCACGTTGACCATGGAGACAATGGCCAACCTAGGGTTGATTTACTACATGTTCGTCCTGGGTTTGCAAGTGGATTTCAAGCCAATATTCCACGCTAGAAAAAAAGTTTTGAGCATTGCCGCTGCTGGTATTCTTCTTCCCCTACCTTTTGGCTATGCCTTGTACAGAGTCCTAACACGACAGGAGGATGGTCTTACTACTGTTACTAAACTAAAGGCTCGTACGTACGGTCCTATATTCTGGGGAATCATTCTTGCCTCCACCAACTTCGGGGAACTCGCCGGAGTACTTGCACACGTGAAGCTCCTCCACTCTGACGTTGGACGAACGGCCTTGTCTGTAAGTGTTACCAGCGACCTCATCTGTTGGATTCTTCTTGTGGGCACAGTGGCCTCAACAAGTGACGGTTATCAAGTTTATACGGTCTTATCCACCTCAGCATTAATAATATTTTTAGTTTTTGTGGTTCGTCCTACCTTGTCATGGATGTTTTCTAACACCAACGACGCTTCTAACGACAATGGTACTTCATTCTACGACATGGATCCTCAAATATGCTTCATTCTAGCCGGGGTTTTATTTTTCGGGTACTTAAGTGATGCATGCGGGGCACACTCCATTCTCGGAGCTTTCACAATGGGAGCCATAGTGCCAAAGGGAGAGCTCAAAAACGCAATTATACAGAAGGTTGACGACTTTGTGTCAAACGTTATGATGTCCCTCTTCTTTTTAGTGCTTGGGATGAGAACGCATGTCCAAGCAGTCATCCTTGACAAAAAAGACATACTATTTGTCTTGCCAGTTATTGTTTTGGCTTCGCTCGCTAAACTTGTTGTAACCTTCGTCGCTGCTATCATTAACAAAATGCCAATGCGCGATAGTTTCGCCCTCGGACTTGTCATGAACACCAAAGGCGTAGTCTCACTAATAATCCTCAATTCTGGCAGGGACTTAAAGGTTTTGGACCACCACACATTTTCAGTAATGATGGCTGCGGTTTGTGTAATGACGGCTGCTGTTGGACCCATTTTGAACCTGATCTACAAGCGCAATGGGACATCTAAGCATTACAAGCATAGAAGCATAAGAAGCATTCAACCAAACTCGGAGTTTCGCATCCTTACTTGCATCCATTCGACAAGCAATGTTTCCGGCGTCATCAATCTCTTGGAGGCTTCAAATCCCACGAAGCAATCCCCAATTTCTGTATTTGCCGTCCACCTGGTGGAGCTAAGAAGTCATGCTTCGGCCATGCTCATAGTGCACGACACATGCAACTTGAATAGGACAACCAGTGCGAGCAGTACAACTAAAAATCAAAATCACGCAAAGCATGCGCACTTTTCGACATCGTCGAACAACATTGTTGCTGCCTTTGAAAAATTTGAGAGTGAGTGTGAAGAAGGCAGCGTGTCTGTCGAGGCACTAACGACTGTGTCTTCTTACACCACCATACACGAGGACATTTGCAACCTTGCCGAGGAAAAACAGGCAGACCTTATAATTGTTCCTTTTCATAAGCAATCCACCATTGACGGAGGAATGGACAGTGGAAACGCTAGTGTTAGAGACGTTAACAGGAATCTTTTAGAGAATGTACCCTGTTCTGTGGCTGTCTTTGTGGATCGTGGCCTCGGCGATTTATCTGAAACTAAAAATGGAAATAACGGTCAAGGGCACCGTCACCACCGCTGTTCCATGCTTTTTATTGGCGGCCCAGACGACCGCGAGGCATTGGCTTATGCGTGGAGGCTGGCTAGCAATCCTATTCCAAACCCTAATATCAGGTTAACTATTGTACGTTTCATTGTCGGTAAGGATGCAACAGTTGGTTCCGATCTCCTTCTCAATAACGATAAGAATAACGGTGACCATCACGAGGAAGAAAACAATAAGATTTTGCAGGACGTAAAGGGAAACGAGAAAGAGAAGCAATTGGACGACCAATGTATAGAGGGCTTTATGCTCAATGCTAAGAACCATCCTTACATAAAACTAATTACGGAGGTAGTGAACAATGGAGAGGAAACTCTCCAACTATTAAGCGACATGGCAAGTGACTATGATCTTTATATTGTGGGAAGAGGGCAAGGAGTTTCCTCACCACTCATCACTTTTGGGCTATCTGAGTGGGGTGACTGTCCAGAGCTAGGACCTTTGGGAGATACGTTGGTGTCGTCCAGATTTGTTGCCAATGCGTCGATTATCATTGTGCATCGAGGTGCTTCTCTTGTGGATGAATCCACTGATCAACATCTTACCCAGCAGTCTATGCATGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

866

Amino Acids

94.64

Weight (kDa)

6.67

Isoelectric Point (pI)

31.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	57 - 449	8.5e-52	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	508 - 653	4.4e-19	Plant cation/H(+) antiporter domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001291)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g25710 FvH4_5g31470 FvH4_7g01800
malus_domestica	MD02G1284400.v1.1 MD02G1284600.v1.1 MD02G1284900.v1.1 MD02G1285000.v1.1 MD02G1285100.v1.1 MD02G1296900.v1.1 MD07G1050200.v1.1 MD07G1050300.v1.1 MD15G1139600.v1.1
prunus_persica	Prupe.2G027500_v2.0.a1 Prupe.2G038300_v2.0.a1 Prupe.2G040900_v2.0.a1 Prupe.4G283300_v2.0.a1 Prupe.4G283400_v2.0.a1 Prupe.4G283500_v2.0.a1 Prupe.4G283500_v2.0.a1 Prupe.4G284600_v2.0.a1 Prupe.4G284900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0318411 RchiOBHm_Chr5g0030741 RchiOBHm_Chr7g0226891
rosa_laevigata	RLG00000001685 RLG00000030540 RLG00000033248
rosa_multiflora	Rmu_co8328645.1_g000001 Rmu_co8411059.1_g000001 Rmu_sc0002662.1_g000016 Rmu_sc0003238.1_g000035 Rmu_sc0003936.1_g000039 Rmu_sc0034904.1_g000001
rosa_roxburghii	Rroxscaffold_3G00232440 Rroxscaffold_4G00329260
rosa_rugosa	Rorug01G0021100 Rorug05G0120500 Rorug07G0240000.1
rosa_samantha	Rh1AG033200 Rh1BG029000 Rh1CG031500 Rh1DG038000 Rh5AG212000 Rh5BG211100 Rh5CG234800 Rh5DG216300 Rh7AG387600 Rh7BG374100 Rh7CG407200 Rh7DG386300
rosa_wichuraiana	Rw0G000940 Rw0G000950 Rw1G002660 Rw5G019460 Rw7G032560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1817
Acc16I	TGCGCA	1 cut(s)	1653
AccB1I	GGYRCC	1 cut(s)	1992
AccB7I	CCANNNNNTGG	1 cut(s)	1540
AccII	CGCG	3 cut(s)	225, 1191, 2043
AciI	CCGC	6 cut(s)	435, 921, 1310, 2005, 2028, 2041
AclI	AACGTT	1 cut(s)	1023
AclWI	GGATC	3 cut(s)	854, 867, 1947
AcoI	YGGCCR	2 cut(s)	339, 1563
AcsI	RAATTY	1 cut(s)	1697
AcvI	CACGTG	1 cut(s)	613
AcyI	GRCGYC	1 cut(s)	1470
AdeI	CACNNNGTG	1 cut(s)	1540
AfiI	CCNNNNNNNGG	7 cut(s)	348, 585, 798, 1540, 1795, 2194, 2465
AflII	CTTAAG	1 cut(s)	907
AflIII	ACRYGT	2 cut(s)	363, 610
AgsI	TTSAA	8 cut(s)	135, 313, 394, 1348, 1412, 1494, 1597, 1694
AhdI	GACNNNNNGTC	1 cut(s)	1015
AjnI	CCWGG	2 cut(s)	372, 1536
AluBI	AGCT	7 cut(s)	164, 196, 619, 944, 979, 1546, 2464
AluI	AGCT	7 cut(s)	164, 196, 619, 944, 979, 1546, 2464
Alw21I	GWGCWC	2 cut(s)	981, 1581
Alw26I	GTCTC	3 cut(s)	326, 1240, 1878
Alw44I	GTGCAC	1 cut(s)	1577
AlwI	GGATC	3 cut(s)	854, 867, 1947
Ama87I	CYCGRG	1 cut(s)	9
AoxI	GGCC	6 cut(s)	339, 645, 705, 1563, 1945, 2029
ApaLI	GTGCAC	1 cut(s)	1577
ApeKI	GCWGC	8 cut(s)	5, 196, 437, 1163, 1307, 1328, 1685, 1722
ApoI	RAATTY	1 cut(s)	1697
ArsI	GACNNNNNNTTYG	4 cut(s)	1088, 1120, 1218, 1250
AseI	ATTAAT	1 cut(s)	758
AspA2I	CCTAGG	1 cut(s)	346
AspLEI	GCGC	3 cut(s)	1191, 1365, 1654
AspS9I	GGNCC	6 cut(s)	260, 545, 1279, 1337, 2030, 2468
AsuC2I	CCSGG	1 cut(s)	886
AsuHPI	GGTGA	4 cut(s)	1991, 2195, 2409, 2462
AsuNHI	GCTAGC	1 cut(s)	2072
AvaI	CYCGRG	1 cut(s)	9
AvaII	GGWCC	5 cut(s)	260, 545, 1279, 1337, 2468
AvrII	CCTAGG	1 cut(s)	346
BaeGI	GKGCMC	5 cut(s)	281, 701, 928, 1581, 1995
BaeI	ACNNNNGTAYC	4 cut(s)	895, 928, 2472, 2505
BalI	TGGCCA	1 cut(s)	341
BamHI	GGATCC	1 cut(s)	859
BanI	GGYRCC	1 cut(s)	1992
BanII	GRGCYC	1 cut(s)	981
BauI	CACGAG	2 cut(s)	1772, 2192
BbrPI	CACGTG	1 cut(s)	613
BbsI	GAAGAC	1 cut(s)	1746
Bbv12I	GWGCWC	2 cut(s)	981, 1581
BbvCI	CCTCAGC	1 cut(s)	751
BbvI	GCAGC	7 cut(s)	183, 424, 1150, 1294, 1315, 1672, 1734
BccI	CCATC	5 cut(s)	271, 497, 1297, 2196, 2304
BceAI	ACGGC	4 cut(s)	297, 660, 1341, 1514
BcgI	CGANNNNNNTGC	2 cut(s)	268, 302
BciT130I	CCWGG	2 cut(s)	374, 1538
BclI	TGATCA	2 cut(s)	168, 2563
BcnI	CCSGG	1 cut(s)	886
BcoDI	GTCTC	3 cut(s)	326, 1240, 1878
BfaI	CTAG	6 cut(s)	347, 411, 881, 1875, 2073, 2465
BfrI	CTTAAG	1 cut(s)	907
BlnI	CCTAGG	1 cut(s)	346
BmcAI	AGTACT	2 cut(s)	42, 603
Bme1390I	CCNGG	3 cut(s)	374, 886, 1538
Bme18I	GGWCC	5 cut(s)	260, 545, 1279, 1337, 2468
BmeRI	GACNNNNNGTC	1 cut(s)	1015
BmeT110I	CYCGRG	1 cut(s)	9
BmgT120I	GGNCC	6 cut(s)	260, 545, 1279, 1337, 2030, 2468
BmiI	GGNNCC	6 cut(s)	261, 861, 958, 1339, 1994, 2149
BmrFI	CCNGG	3 cut(s)	374, 886, 1538
BmsI	GCATC	5 cut(s)	904, 1440, 1452, 2125, 2539
BmtI	GCTAGC	1 cut(s)	2076
BpiI	GAAGAC	1 cut(s)	1746
BpmI	CTGGAG	1 cut(s)	65
Bpu10I	CCTNAGC	1 cut(s)	751
BpuEI	CTTGAG	1 cut(s)	176
BpuMI	CCSGG	1 cut(s)	886
BsaAI	YACGTR	3 cut(s)	301, 540, 613
BsaBI	GATNNNNATC	1 cut(s)	2568
BsaHI	GRCGYC	1 cut(s)	1470
BsaJI	CCNNGG	7 cut(s)	327, 346, 373, 885, 1204, 1794, 1948
BsaXI	ACNNNNNCTCC	2 cut(s)	948, 978
Bsc4I	CCNNNNNNNGG	7 cut(s)	348, 585, 798, 1540, 1795, 2194, 2465
Bse1I	ACTGG	4 cut(s)	48, 106, 1115, 1608
Bse3DI	GCAATG	4 cut(s)	34, 429, 1372, 1465
Bse8I	GATNNNNATC	1 cut(s)	2568
BseBI	CCWGG	2 cut(s)	374, 1538
BseDI	CCNNGG	7 cut(s)	327, 346, 373, 885, 1204, 1794, 1948
BseJI	GATNNNNATC	1 cut(s)	2568
BseLI	CCNNNNNNNGG	7 cut(s)	348, 585, 798, 1540, 1795, 2194, 2465
BseMI	GCAATG	4 cut(s)	34, 429, 1372, 1465
BseMII	CTCAG	2 cut(s)	765, 2433
BseNI	ACTGG	4 cut(s)	48, 106, 1115, 1608
BseRI	GAGGAG	1 cut(s)	611
BseSI	GKGCMC	5 cut(s)	281, 701, 928, 1581, 1995
BseXI	GCAGC	7 cut(s)	183, 424, 1150, 1294, 1315, 1672, 1734
BseYI	CCCAGC	1 cut(s)	2577
Bsh1236I	CGCG	3 cut(s)	225, 1191, 2043
Bsh1285I	CGRYCG	1 cut(s)	2041
BshFI	GGCC	6 cut(s)	341, 647, 707, 1565, 1947, 2031
BshNI	GGYRCC	1 cut(s)	1992
BsiEI	CGRYCG	1 cut(s)	2041
BsiHKAI	GWGCWC	2 cut(s)	981, 1581
BsiHKCI	CYCGRG	1 cut(s)	9
BsiSI	CCGG	3 cut(s)	597, 885, 1467
BsiWI	CGTACG	2 cut(s)	536, 540
BslFI	GGGAC	4 cut(s)	160, 1018, 1277, 1384
BslI	CCNNNNNNNGG	7 cut(s)	348, 585, 798, 1540, 1795, 2194, 2465
BsmAI	GTCTC	3 cut(s)	326, 1240, 1878
BsmBI	CGTCTC	1 cut(s)	1878
BsmFI	GGGAC	4 cut(s)	160, 1018, 1277, 1384
BsmI	GAATGC	1 cut(s)	1407
BsnI	GGCC	6 cut(s)	341, 647, 707, 1565, 1947, 2031
BsoBI	CYCGRG	1 cut(s)	9
Bsp1286I	GDGCHC	6 cut(s)	281, 701, 928, 981, 1581, 1995
Bsp1407I	TGTACA	1 cut(s)	477
Bsp143I	GATC	7 cut(s)	168, 859, 1353, 1939, 2152, 2380, 2563
Bsp19I	CCATGG	1 cut(s)	327
BspACI	CCGC	6 cut(s)	435, 921, 1310, 2005, 2028, 2041
BspANI	GGCC	6 cut(s)	341, 647, 707, 1565, 1947, 2031
BspCNI	CTCAG	2 cut(s)	764, 2434
BspFNI	CGCG	3 cut(s)	225, 1191, 2043
BspHI	TCATGA	1 cut(s)	1215
BspLI	GGNNCC	6 cut(s)	261, 861, 958, 1339, 1994, 2149
BspOI	GCTAGC	1 cut(s)	2076
BspPI	GGATC	3 cut(s)	854, 867, 1947
BspT107I	GGYRCC	1 cut(s)	1992
BspTI	CTTAAG	1 cut(s)	907
BsrDI	GCAATG	4 cut(s)	34, 429, 1372, 1465
BsrGI	TGTACA	1 cut(s)	477
BsrI	ACTGG	4 cut(s)	48, 106, 1115, 1608
BssECI	CCNNGG	7 cut(s)	327, 346, 373, 885, 1204, 1794, 1948
BssMI	GATC	7 cut(s)	168, 859, 1353, 1939, 2152, 2380, 2563
BssNI	GRCGYC	1 cut(s)	1470
BssSI	CACGAG	2 cut(s)	1772, 2192
BssT1I	CCWWGG	2 cut(s)	327, 346
Bst2BI	CACGAG	2 cut(s)	1772, 2192
Bst2UI	CCWGG	2 cut(s)	374, 1538
Bst6I	CTCTTC	4 cut(s)	144, 152, 1043, 2392
BstACI	GRCGYC	1 cut(s)	1470
BstAFI	CTTAAG	1 cut(s)	907
BstAPI	GCANNNNNTGC	2 cut(s)	1649, 2587
BstAUI	TGTACA	1 cut(s)	477
BstBAI	YACGTR	3 cut(s)	301, 540, 613
BstC8I	GCNNGC	7 cut(s)	292, 919, 1137, 1615, 1650, 2070, 2074
BstDEI	CTNAG	5 cut(s)	751, 1377, 1547, 2289, 2442
BstDSI	CCRYGG	1 cut(s)	327
BstEII	GGTNACC	1 cut(s)	2183
BstENI	CCTNNNNNAGG	1 cut(s)	1793
BstFNI	CGCG	3 cut(s)	225, 1191, 2043
BstHHI	GCGC	3 cut(s)	1191, 1365, 1654
BstKTI	GATC	7 cut(s)	171, 862, 1356, 1942, 2155, 2383, 2566
BstMAI	GTCTC	3 cut(s)	326, 1240, 1878
BstMBI	GATC	7 cut(s)	168, 859, 1353, 1939, 2152, 2380, 2563
BstMCI	CGRYCG	1 cut(s)	2041
BstMWI	GCNNNNNNNGC	8 cut(s)	122, 923, 1136, 1387, 1404, 1576, 1649, 2587
BstNI	CCWGG	2 cut(s)	374, 1538
BstNSI	RCATGY	6 cut(s)	367, 921, 1070, 1590, 1652, 2594
BstPI	GGTNACC	1 cut(s)	2183
BstSCI	CCNGG	3 cut(s)	372, 884, 1536
BstSLI	GKGCMC	5 cut(s)	281, 701, 928, 1581, 1995
BstSNI	TACGTA	2 cut(s)	301, 540
BstUI	CGCG	3 cut(s)	225, 1191, 2043
BstV1I	GCAGC	7 cut(s)	183, 424, 1150, 1294, 1315, 1672, 1734
BstV2I	GAAGAC	1 cut(s)	1746
BstX2I	RGATCY	1 cut(s)	859
BstYI	RGATCY	1 cut(s)	859
BsuRI	GGCC	6 cut(s)	341, 647, 707, 1565, 1947, 2031
BtgI	CCRYGG	1 cut(s)	327
BtsIMutI	CAGTG	5 cut(s)	99, 708, 1615, 1870, 2559
Cac8I	GCNNGC	7 cut(s)	292, 919, 1137, 1615, 1650, 2070, 2074
CciI	TCATGA	1 cut(s)	1215
CfoI	GCGC	3 cut(s)	1191, 1365, 1654
Cfr13I	GGNCC	6 cut(s)	260, 545, 1279, 1337, 2030, 2468
CseI	GACGC	3 cut(s)	830, 1459, 2502
CsiI	ACCWGGT	1 cut(s)	1536
DdeI	CTNAG	5 cut(s)	751, 1377, 1547, 2289, 2442
DpnI	GATC	7 cut(s)	170, 861, 1355, 1941, 2154, 2382, 2565
DpnII	GATC	7 cut(s)	168, 859, 1353, 1939, 2152, 2380, 2563
DraIII	CACNNNGTG	1 cut(s)	1540
DriI	GACNNNNNGTC	1 cut(s)	1015
EaeI	YGGCCR	2 cut(s)	339, 1563
Eam1104I	CTCTTC	4 cut(s)	144, 152, 1043, 2392
Eam1105I	GACNNNNNGTC	1 cut(s)	1015
EarI	CTCTTC	4 cut(s)	144, 152, 1043, 2392
Ecl136II	GAGCTC	1 cut(s)	979
Eco105I	TACGTA	2 cut(s)	301, 540
Eco130I	CCWWGG	2 cut(s)	327, 346
Eco24I	GRGCYC	1 cut(s)	981
Eco47I	GGWCC	5 cut(s)	260, 545, 1279, 1337, 2468
Eco53kI	GAGCTC	1 cut(s)	979
Eco72I	CACGTG	1 cut(s)	613
Eco88I	CYCGRG	1 cut(s)	9
Eco91I	GGTNACC	1 cut(s)	2183
EcoICRI	GAGCTC	1 cut(s)	979
EcoNI	CCTNNNNNAGG	1 cut(s)	1793
EcoO109I	RGGNCCY	1 cut(s)	2468
EcoO65I	GGTNACC	1 cut(s)	2183
EcoRII	CCWGG	2 cut(s)	372, 1536
EcoT14I	CCWWGG	2 cut(s)	327, 346
EcoT22I	ATGCAT	2 cut(s)	919, 2592
EcoT38I	GRGCYC	1 cut(s)	981
ErhI	CCWWGG	2 cut(s)	327, 346
Esp3I	CGTCTC	1 cut(s)	1878
FalI	AAGNNNNNCTT	2 cut(s)	1543, 1575
FaqI	GGGAC	4 cut(s)	160, 1018, 1277, 1384
FauI	CCCGC	1 cut(s)	914
FbaI	TGATCA	2 cut(s)	168, 2563
FriOI	GRGCYC	1 cut(s)	981
FspAI	RTGCGCAY	1 cut(s)	1653
FspBI	CTAG	6 cut(s)	347, 411, 881, 1875, 2073, 2465
FspI	TGCGCA	1 cut(s)	1653
GlaI	GCGC	3 cut(s)	1190, 1364, 1653
GsaI	CCCAGC	1 cut(s)	2581
GsuI	CTGGAG	1 cut(s)	65
HaeIII	GGCC	6 cut(s)	341, 647, 707, 1565, 1947, 2031
HapII	CCGG	3 cut(s)	597, 885, 1467
HgaI	GACGC	3 cut(s)	830, 1459, 2502
HhaI	GCGC	3 cut(s)	1191, 1365, 1654
Hin1I	GRCGYC	1 cut(s)	1470
Hin6I	GCGC	3 cut(s)	1189, 1363, 1652
HinP1I	GCGC	3 cut(s)	1189, 1363, 1652
HincII	GTYRAC	5 cut(s)	17, 324, 1006, 1891, 2106
HindII	GTYRAC	5 cut(s)	17, 324, 1006, 1891, 2106
HinfI	GANTC	5 cut(s)	483, 561, 685, 1897, 2555
HpaI	GTTAAC	2 cut(s)	1891, 2106
HpaII	CCGG	3 cut(s)	597, 885, 1467
HphI	GGTGA	4 cut(s)	1991, 2195, 2409, 2462
Hpy166II	GTNNAC	8 cut(s)	17, 324, 1006, 1534, 1579, 1891, 2106, 2329
Hpy188I	TCNGA	7 cut(s)	631, 941, 1208, 1423, 1963, 2152, 2443
Hpy188III	TCNNGA	4 cut(s)	1216, 2192, 2459, 2496
Hpy8I	GTNNAC	8 cut(s)	17, 324, 1006, 1534, 1579, 1891, 2106, 2329
Hpy99I	CGWCG	5 cut(s)	24, 824, 1163, 1672, 2518
HpyAV	CCTTC	6 cut(s)	106, 197, 994, 1165, 1712, 2168
HpyF10VI	GCNNNNNNNGC	8 cut(s)	122, 923, 1136, 1387, 1404, 1576, 1649, 2587
HpyF3I	CTNAG	5 cut(s)	751, 1377, 1547, 2289, 2442
Hsp92I	GRCGYC	1 cut(s)	1470
HspAI	GCGC	3 cut(s)	1189, 1363, 1652
Ksp22I	TGATCA	2 cut(s)	168, 2563
KspAI	GTTAAC	2 cut(s)	1891, 2106
Kzo9I	GATC	7 cut(s)	168, 859, 1353, 1939, 2152, 2380, 2563
LmnI	GCTCC	4 cut(s)	624, 941, 956, 1543
Lsp1109I	GCAGC	7 cut(s)	183, 424, 1150, 1294, 1315, 1672, 1734
LweI	GCATC	5 cut(s)	904, 1440, 1452, 2125, 2539
MabI	ACCWGGT	1 cut(s)	1536
MaeI	CTAG	6 cut(s)	347, 411, 881, 1875, 2073, 2465
MaeIII	GTNAC	9 cut(s)	182, 517, 661, 716, 1150, 1997, 2183, 2372, 2450
MalI	GATC	7 cut(s)	170, 861, 1355, 1941, 2154, 2382, 2565
MboI	GATC	7 cut(s)	168, 859, 1353, 1939, 2152, 2380, 2563
MfeI	CAATTG	1 cut(s)	2249
MflI	RGATCY	1 cut(s)	859
MhlI	GDGCHC	6 cut(s)	281, 701, 928, 981, 1581, 1995
MlsI	TGGCCA	1 cut(s)	341
MluCI	AATT	7 cut(s)	990, 1252, 1515, 1697, 1818, 2249, 2313
MluNI	TGGCCA	1 cut(s)	341
MlyI	GAGTC	1 cut(s)	492
MmeI	TCCRAC	5 cut(s)	181, 616, 662, 1315, 2374
Mox20I	TGGCCA	1 cut(s)	341
Mph1103I	ATGCAT	2 cut(s)	919, 2592
MscI	TGGCCA	1 cut(s)	341
MseI	TTAA	8 cut(s)	78, 758, 908, 1173, 1268, 1890, 2105, 2357
MslI	CAYNNNNRTG	1 cut(s)	2535
Msp20I	TGGCCA	1 cut(s)	341
MspA1I	CMGCKG	2 cut(s)	437, 2007
MspCI	CTTAAG	1 cut(s)	907
MspI	CCGG	3 cut(s)	597, 885, 1467
MspR9I	CCNGG	3 cut(s)	374, 886, 1538
MunI	CAATTG	1 cut(s)	2249
Mva1269I	GAATGC	1 cut(s)	1407
MvaI	CCWGG	2 cut(s)	374, 1538
MvnI	CGCG	3 cut(s)	225, 1191, 2043
MwoI	GCNNNNNNNGC	8 cut(s)	122, 923, 1136, 1387, 1404, 1576, 1649, 2587
NciI	CCSGG	1 cut(s)	886
NcoI	CCATGG	1 cut(s)	327
NdeII	GATC	7 cut(s)	168, 859, 1353, 1939, 2152, 2380, 2563
NheI	GCTAGC	1 cut(s)	2072
NlaIV	GGNNCC	6 cut(s)	261, 861, 958, 1339, 1994, 2149
NmeAIII	GCCGAG	2 cut(s)	1819, 1929
NmuCI	GTSAC	5 cut(s)	716, 1997, 2183, 2372, 2450
NsbI	TGCGCA	1 cut(s)	1653
NsiI	ATGCAT	2 cut(s)	919, 2592
NspI	RCATGY	6 cut(s)	367, 921, 1070, 1590, 1652, 2594
PaeI	GCATGC	2 cut(s)	921, 1652
PagI	TCATGA	1 cut(s)	1215
PciI	ACATGT	1 cut(s)	363
PcsI	WCGNNNNNNNCGW	4 cut(s)	16, 828, 1667, 2489
PctI	GAATGC	1 cut(s)	1407
PfeI	GAWTC	4 cut(s)	561, 685, 1897, 2555
Pfl23II	CGTACG	2 cut(s)	536, 540
PflFI	GACNNNGTC	2 cut(s)	1666, 1750
PflMI	CCANNNNNTGG	1 cut(s)	1540
PleI	GAGTC	1 cut(s)	491
PmaCI	CACGTG	1 cut(s)	613
PmlI	CACGTG	1 cut(s)	613
PpsI	GAGTC	1 cut(s)	491
Ppu21I	YACGTR	3 cut(s)	301, 540, 613
PpuMI	RGGWCCY	1 cut(s)	2468
PscI	ACATGT	1 cut(s)	363
PshBI	ATTAAT	1 cut(s)	758
PsiI	TTATAA	1 cut(s)	1817
Psp124BI	GAGCTC	1 cut(s)	981
Psp1406I	AACGTT	1 cut(s)	1023
Psp5II	RGGWCCY	1 cut(s)	2468
Psp6I	CCWGG	2 cut(s)	372, 1536
PspCI	CACGTG	1 cut(s)	613
PspEI	GGTNACC	1 cut(s)	2183
PspFI	CCCAGC	1 cut(s)	2577
PspGI	CCWGG	2 cut(s)	372, 1536
PspLI	CGTACG	2 cut(s)	536, 540
PspN4I	GGNNCC	6 cut(s)	261, 861, 958, 1339, 1994, 2149
PspPI	GGNCC	6 cut(s)	260, 545, 1279, 1337, 2030, 2468
PspPPI	RGGWCCY	1 cut(s)	2468
PsrI	GAACNNNNNNTAC	4 cut(s)	1340, 1372, 2285, 2317
PsuI	RGATCY	1 cut(s)	859
PsyI	GACNNNGTC	2 cut(s)	1666, 1750
RseI	CAYNNNNRTG	1 cut(s)	2535
SacI	GAGCTC	1 cut(s)	981
SaqAI	TTAA	8 cut(s)	78, 758, 908, 1173, 1268, 1890, 2105, 2357
Sau3AI	GATC	7 cut(s)	168, 859, 1353, 1939, 2152, 2380, 2563
Sau96I	GGNCC	6 cut(s)	260, 545, 1279, 1337, 2030, 2468
ScaI	AGTACT	2 cut(s)	42, 603
SchI	GAGTC	1 cut(s)	492
ScrFI	CCNGG	3 cut(s)	374, 886, 1538
SduI	GDGCHC	6 cut(s)	281, 701, 928, 981, 1581, 1995
SexAI	ACCWGGT	1 cut(s)	1536
SfaNI	GCATC	5 cut(s)	904, 1440, 1452, 2125, 2539
SinI	GGWCC	5 cut(s)	260, 545, 1279, 1337, 2468
SmiMI	CAYNNNNRTG	1 cut(s)	2535
SmlI	CTYRAG	2 cut(s)	191, 907
SmoI	CTYRAG	2 cut(s)	191, 907
SnaBI	TACGTA	2 cut(s)	301, 540
SphI	GCATGC	2 cut(s)	921, 1652
Sse9I	AATT	7 cut(s)	990, 1252, 1515, 1697, 1818, 2249, 2313
SsiI	CCGC	6 cut(s)	435, 921, 1310, 2005, 2028, 2041
SspI	AATATT	2 cut(s)	402, 765
SspMI	CTAG	6 cut(s)	347, 411, 881, 1875, 2073, 2465
SstI	GAGCTC	1 cut(s)	981
StyD4I	CCNGG	3 cut(s)	372, 884, 1536
StyI	CCWWGG	2 cut(s)	327, 346
TaqI	TCGA	6 cut(s)	1451, 1661, 1670, 1734, 2516, 2533
TasI	AATT	7 cut(s)	990, 1252, 1515, 1697, 1818, 2249, 2313
TatI	WGTACW	4 cut(s)	40, 477, 601, 1619
TauI	GCSGC	2 cut(s)	437, 2031
TfiI	GAWTC	4 cut(s)	561, 685, 1897, 2555
Tru1I	TTAA	8 cut(s)	78, 758, 908, 1173, 1268, 1890, 2105, 2357
Tru9I	TTAA	8 cut(s)	78, 758, 908, 1173, 1268, 1890, 2105, 2357
TscAI	CASTG	5 cut(s)	106, 708, 1615, 1870, 2566
TseFI	GTSAC	5 cut(s)	716, 1997, 2183, 2372, 2450
TseI	GCWGC	8 cut(s)	5, 196, 437, 1163, 1307, 1328, 1685, 1722
Tsp45I	GTSAC	5 cut(s)	716, 1997, 2183, 2372, 2450
TspDTI	ATGAA	7 cut(s)	142, 834, 865, 1232, 1820, 2110, 2568
TspGWI	ACGGA	2 cut(s)	1866, 2333
TspRI	CASTG	5 cut(s)	106, 708, 1615, 1870, 2566
Tth111I	GACNNNGTC	2 cut(s)	1666, 1750
Van91I	CCANNNNNTGG	1 cut(s)	1540
Vha464I	CTTAAG	1 cut(s)	907
VneI	GTGCAC	1 cut(s)	1577
VpaK11BI	GGWCC	5 cut(s)	260, 545, 1279, 1337, 2468
VspI	ATTAAT	1 cut(s)	758
XagI	CCTNNNNNAGG	1 cut(s)	1793
XapI	RAATTY	1 cut(s)	1697
XceI	RCATGY	6 cut(s)	367, 921, 1070, 1590, 1652, 2594
XcmI	CCANNNNNNNNNTGG	2 cut(s)	334, 632
XmaJI	CCTAGG	1 cut(s)	346
XspI	CTAG	6 cut(s)	347, 411, 881, 1875, 2073, 2465
ZrmI	AGTACT	2 cut(s)	42, 603
Zsp2I	ATGCAT	2 cut(s)	919, 2592

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.