Rorug05G0120500

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001291
Physical Location & Seq
Forward (+)
10620537 .. 10624428
3892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0120500.1

Sequence Viewer

Length: 1578 bp
ATGAACAGGAACAAGAAGCGAGGCGGCGGCGGCGGGGGAGGCTCAGCTCCCAAGGGCGGAAGAGGGGGAGACAGAAAGGGAGCTAAAAATGGCAAGAGGCAGTCCTTCAAGGAAGACCCATTTTTCACCAACGAATCCAAGAAGCGGCTCAAATACGACGAGGACATTCGTAGCGACGAGTCGTCCGACGACGACCGAAACGACGCCGTCGGTAGTGGCGAGGAGGAGGAGGAGGCGGCTGAGGAGATTATAGAGGAGACTCCCGGGGAGAAGAAGCTGAGGCTGACGAAGGAGTATCTGAAGAGGCTTGGAGAACATGAGAGGAAGAAGAAGGAAGACGAGGATGACGATAGCGATGACGACAGCGAGAAGGAAGGGGACAGAGACTCGCTTGTGGCCAAGGTTTTGCAGCAGCAGCAGCTCCAGGACAGCGGCCGCCTCCGCCGCTCCATTGCTTCTAGGGTTCAGAAGCCAGAAGCTACTGATGAGTTTCGGATCTTAGTGAAGCATCGCCAATCTGTCACCGCAGTGGTTCTCTCTGACGATGATGTCAGAGGTTTTTCAGCATCTAAGGACGGCGCGATTTTGCATTGGGATGTAGATAGTGGGAAAAAAGAGAAGTATCAGTGGCCCAGTGACGAAGTATTGAAGTCCCACGGGGTCAAGGATCCACTAGGTAGAGCTACAAAGCATAGCAAACAACTGTTAGCACTTGCAGTTAGTTCTGATGGTCGGTATTTGGCAAGTGGAGGATTAGATCGCCATATTCATTTGTGGGATACTCGTACCAGACAGCATGTTCAGGCTTTTCCGGGTCACAGGGGACCTGTATCATGTTTAACTTTCAGGCAAGGGAGTTCAGAACTCTTTTCTGGTTCATATGATCGAACAGTTAAGATATGGAATGCAGAAGATAGAACTTACATAAATACATTATATGGTCACCAAGGTGAAGTATTATCTATGGATTGCCTACGGAAAGAACGGCTTTTGACTGTTGGACATGATCGAAGCATGATGTTATTCAAGGTTCCGGAGGAGTCGCGTTTAGTATTCCGTGCCCCTGCATGCTCATTGGAGTGTTGTTCTTTTATTAGCAATGATGAATTCTTATCTGGCTCTGATAATGGAAGTGTTGAGCTTTGGACCATGCTGCGAAAGAAGCCTGTATGCATAGTTAAGAATGCTCATCCTTTGTTAGCTGCACACAAAGATGTTGAACAGAAGGATGGTGATAGAGTCCCCAATGGTCACATAGAAAGCTATGATTGTTCATCAACATATTCTTGGATCAGTTCAGTCACTTCTTGTCGAGGCAGTGACCTTGCCGCATCAGGAGCTGGTAATGGCTTTGTTCGTTTATGGTCTATTGAAAGTGAGAGCAATGCCATTCAGCCCTTACACAGTCTCCCCGTGGTTGGATTTGTTAATTCTTTAGCTATTGCAAAATCTGGAGGCTTTCTAGTTGCAGGAGTCGGAAAGGAACCGCGTCTAGGAAGGTGGGGACTTATCCGAGCTGCTCAGAATGGAGTTGCAATCCACACCCTTAAGCTTTCGTCAGATACAAATAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

525

Amino Acids

58.15

Weight (kDa)

6.98

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 160 - 345 5.6e-17 CDC20/Fizzy WD40 domain
WDR55 PF24796 161 - 393 2e-13 WDR55
Beta-prop_WDR36-Utp21_1st PF25171 190 - 340 9.8e-07 WDR36/Utp21 first beta-propeller
NBCH_WD40 PF20426 202 - 327 2.2e-06 Neurobeachin beta propeller domain
Beta-prop_TEP1_2nd PF25047 210 - 339 2e-12 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 225 - 284 3e-10 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 227 - 343 3.1e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 229 - 260 2e-06 WD domain, G-beta repeat
Beta-prop_EML PF23409 229 - 397 5.9e-11 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 229 - 389 3.8e-23 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 230 - 344 2.6e-23 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 231 - 384 1.3e-10 G protein beta WD-40 repeat protein
EIF3I PF24805 231 - 311 7.7e-06 EIF3I
WD40_MABP1-WDR62_2nd PF24782 231 - 347 6.5e-09 MABP1/WDR62 second WD40 domain
WD40_MABP1-WDR62_1st PF24780 232 - 307 6.6e-06 MABP1/WDR62 first WD40 domain
WD40_Prp19 PF24814 233 - 391 5.4e-19 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 234 - 347 3.5e-15 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 237 - 336 2e-06 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 266 - 302 2.5e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 273 - 345 5e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 548
AccBSI CCGCTC 1 cut(s) 447
AccII CGCG 3 cut(s) 581, 1045, 1489
AccIII TCCGGA 1 cut(s) 1033
AclWI GGATC 4 cut(s) 503, 662, 675, 1298
AcoI YGGCCR 2 cut(s) 396, 433
AcsI RAATTY 1 cut(s) 1106
AcuI CTGAAG 1 cut(s) 320
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 1 cut(s) 787
AfiI CCNNNNNNNGG 4 cut(s) 56, 144, 1418, 1493
AflII CTTAAG 1 cut(s) 1547
AgsI TTSAA 5 cut(s) 109, 649, 1027, 1220, 1373
AhdI GACNNNNNGTC 1 cut(s) 181
AjnI CCWGG 1 cut(s) 423
AleI CACNNNNGTG 2 cut(s) 527, 948
Alw26I GTCTC 4 cut(s) 63, 251, 378, 1412
AlwI GGATC 4 cut(s) 503, 662, 675, 1298
AlwNI CAGNNNCTG 1 cut(s) 1340
Ama87I CYCGRG 1 cut(s) 263
Aor13HI TCCGGA 1 cut(s) 1033
AoxI GGCC 3 cut(s) 396, 433, 629
ApeKI GCWGC 7 cut(s) 409, 412, 415, 418, 1153, 1202, 1517
ApoI RAATTY 1 cut(s) 1106
AspLEI GCGC 1 cut(s) 581
AspS9I GGNCC 3 cut(s) 630, 824, 1146
AsuC2I CCSGG 3 cut(s) 264, 265, 813
AsuHPI GGTGA 5 cut(s) 118, 514, 935, 962, 1244
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 2 cut(s) 824, 1146
BaeGI GKGCMC 1 cut(s) 1063
BalI TGGCCA 1 cut(s) 398
BamHI GGATCC 1 cut(s) 667
BbsI GAAGAC 2 cut(s) 120, 342
BbvCI CCTCAGC 2 cut(s) 240, 278
BbvI GCAGC 7 cut(s) 421, 424, 427, 430, 1140, 1189, 1504
BccI CCATC 2 cut(s) 722, 1223
BceAI ACGGC 3 cut(s) 191, 592, 1001
BciT130I CCWGG 1 cut(s) 425
BciVI GTATCC 1 cut(s) 772
BcnI CCSGG 3 cut(s) 264, 265, 813
BcoDI GTCTC 4 cut(s) 63, 251, 378, 1412
BfaI CTAG 4 cut(s) 459, 674, 1463, 1493
BfrI CTTAAG 1 cut(s) 1547
BfuI GTATCC 1 cut(s) 772
BlpI GCTNAGC 1 cut(s) 43
Bme1390I CCNGG 4 cut(s) 264, 265, 425, 813
Bme18I GGWCC 2 cut(s) 824, 1146
BmeRI GACNNNNNGTC 1 cut(s) 181
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 3 cut(s) 630, 824, 1146
BmiI GGNNCC 4 cut(s) 669, 825, 1032, 1485
BmrFI CCNGG 4 cut(s) 264, 265, 425, 813
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 3 cut(s) 517, 575, 1340
BmuI ACTGGG 1 cut(s) 627
BpiI GAAGAC 2 cut(s) 120, 342
BpmI CTGGAG 2 cut(s) 407, 1473
Bpu10I CCTNAGC 2 cut(s) 240, 278
Bpu1102I GCTNAGC 1 cut(s) 43
BpuMI CCSGG 3 cut(s) 264, 265, 813
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 7 cut(s) 51, 263, 264, 399, 655, 946, 1413
BsaWI WCCGGW 1 cut(s) 1033
BsaXI ACNNNNNCTCC 2 cut(s) 1392, 1422
Bsc4I CCNNNNNNNGG 4 cut(s) 56, 144, 1418, 1493
Bse1I ACTGG 1 cut(s) 633
Bse3DI GCAATG 3 cut(s) 450, 1105, 1390
BseAI TCCGGA 1 cut(s) 1033
BseBI CCWGG 1 cut(s) 425
BseDI CCNNGG 7 cut(s) 51, 263, 264, 399, 655, 946, 1413
BseGI GGATG 4 cut(s) 349, 601, 1189, 1234
BseLI CCNNNNNNNGG 4 cut(s) 56, 144, 1418, 1493
BseMI GCAATG 3 cut(s) 450, 1105, 1390
BseMII CTCAG 4 cut(s) 57, 231, 269, 1535
BseNI ACTGG 1 cut(s) 633
BseRI GAGGAG 7 cut(s) 236, 239, 242, 245, 257, 269, 1052
BseSI GKGCMC 1 cut(s) 1063
BseX3I CGGCCG 1 cut(s) 433
BseXI GCAGC 7 cut(s) 421, 424, 427, 430, 1140, 1189, 1504
BsgI GTGCAG 1 cut(s) 1188
Bsh1236I CGCG 3 cut(s) 581, 1045, 1489
Bsh1285I CGRYCG 2 cut(s) 196, 436
BshFI GGCC 3 cut(s) 398, 435, 631
BsiEI CGRYCG 2 cut(s) 196, 436
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 3 cut(s) 264, 812, 1034
BslFI GGGAC 5 cut(s) 392, 637, 837, 1226, 1518
BslI CCNNNNNNNGG 4 cut(s) 56, 144, 1418, 1493
BsmAI GTCTC 4 cut(s) 63, 251, 378, 1412
BsmFI GGGAC 5 cut(s) 392, 637, 837, 1226, 1518
BsmI GAATGC 2 cut(s) 910, 1189
BsnI GGCC 3 cut(s) 398, 435, 631
BsoBI CYCGRG 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 1063
Bsp13I TCCGGA 1 cut(s) 1033
Bsp143I GATC 6 cut(s) 495, 667, 757, 883, 1006, 1290
Bsp1720I GCTNAGC 1 cut(s) 43
BspANI GGCC 3 cut(s) 398, 435, 631
BspCNI CTCAG 4 cut(s) 56, 232, 270, 1534
BspEI TCCGGA 1 cut(s) 1033
BspFNI CGCG 3 cut(s) 581, 1045, 1489
BspLI GGNNCC 4 cut(s) 669, 825, 1032, 1485
BspPI GGATC 4 cut(s) 503, 662, 675, 1298
BspTI CTTAAG 1 cut(s) 1547
BsrBI CCGCTC 1 cut(s) 447
BsrDI GCAATG 3 cut(s) 450, 1105, 1390
BsrI ACTGG 1 cut(s) 633
BssECI CCNNGG 7 cut(s) 51, 263, 264, 399, 655, 946, 1413
BssMI GATC 6 cut(s) 495, 667, 757, 883, 1006, 1290
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 3 cut(s) 51, 399, 946
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 4 cut(s) 705, 892, 997, 1406
Bst6I CTCTTC 2 cut(s) 55, 296
BstACI GRCGYC 1 cut(s) 204
BstAFI CTTAAG 1 cut(s) 1547
BstC8I GCNNGC 1 cut(s) 1069
BstDEI CTNAG 6 cut(s) 43, 240, 278, 499, 570, 1521
BstDSI CCRYGG 2 cut(s) 655, 1413
BstEII GGTNACC 1 cut(s) 941
BstF5I GGATG 4 cut(s) 349, 601, 1189, 1234
BstFNI CGCG 3 cut(s) 581, 1045, 1489
BstHHI GCGC 1 cut(s) 581
BstKTI GATC 6 cut(s) 498, 670, 760, 886, 1009, 1293
BstMAI GTCTC 4 cut(s) 63, 251, 378, 1412
BstMBI GATC 6 cut(s) 495, 667, 757, 883, 1006, 1290
BstMCI CGRYCG 2 cut(s) 196, 436
BstMWI GCNNNNNNNGC 8 cut(s) 30, 39, 415, 418, 441, 444, 1162, 1337
BstNI CCWGG 1 cut(s) 425
BstNSI RCATGY 2 cut(s) 800, 1071
BstPI GGTNACC 1 cut(s) 941
BstSCI CCNGG 4 cut(s) 262, 263, 423, 811
BstSLI GKGCMC 1 cut(s) 1063
BstUI CGCG 3 cut(s) 581, 1045, 1489
BstV1I GCAGC 7 cut(s) 421, 424, 427, 430, 1140, 1189, 1504
BstV2I GAAGAC 2 cut(s) 120, 342
BstX2I RGATCY 2 cut(s) 495, 667
BstYI RGATCY 2 cut(s) 495, 667
BstZI CGGCCG 1 cut(s) 433
BsuI GTATCC 1 cut(s) 772
BsuRI GGCC 3 cut(s) 398, 435, 631
BtgI CCRYGG 2 cut(s) 655, 1413
BtgZI GCGATG 2 cut(s) 369, 494
BtsCI GGATG 4 cut(s) 349, 601, 1189, 1234
BtsI GCAGTG 2 cut(s) 534, 1324
BtsIMutI CAGTG 4 cut(s) 534, 632, 640, 1324
Cac8I GCNNGC 1 cut(s) 1069
CaiI CAGNNNCTG 1 cut(s) 1340
CciNI GCGGCCGC 1 cut(s) 433
CfoI GCGC 1 cut(s) 581
Cfr13I GGNCC 3 cut(s) 630, 824, 1146
Cfr9I CCCGGG 1 cut(s) 263
CseI GACGC 2 cut(s) 212, 1478
Csp6I GTAC 1 cut(s) 786
CviAII CATG 7 cut(s) 317, 797, 834, 1004, 1015, 1068, 1150
CviQI GTAC 1 cut(s) 786
DdeI CTNAG 6 cut(s) 43, 240, 278, 499, 570, 1521
DpnI GATC 6 cut(s) 497, 669, 759, 885, 1008, 1292
DpnII GATC 6 cut(s) 495, 667, 757, 883, 1006, 1290
DrdI GACNNNNNNGTC 1 cut(s) 548
DriI GACNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 548
EaeI YGGCCR 2 cut(s) 396, 433
EagI CGGCCG 1 cut(s) 433
Eam1104I CTCTTC 2 cut(s) 55, 296
Eam1105I GACNNNNNGTC 1 cut(s) 181
EarI CTCTTC 2 cut(s) 55, 296
EciI GGCGGA 2 cut(s) 72, 431
EclXI CGGCCG 1 cut(s) 433
Eco130I CCWWGG 3 cut(s) 51, 399, 946
Eco47I GGWCC 2 cut(s) 824, 1146
Eco52I CGGCCG 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 263
Eco91I GGTNACC 1 cut(s) 941
EcoO109I RGGNCCY 1 cut(s) 824
EcoO65I GGTNACC 1 cut(s) 941
EcoRI GAATTC 1 cut(s) 1106
EcoRII CCWGG 1 cut(s) 423
EcoT14I CCWWGG 3 cut(s) 51, 399, 946
EcoT22I ATGCAT 1 cut(s) 1175
ErhI CCWWGG 3 cut(s) 51, 399, 946
FaeI CATG 7 cut(s) 320, 800, 837, 1007, 1018, 1071, 1153
FalI AAGNNNNNCTT 2 cut(s) 972, 1004
FaqI GGGAC 5 cut(s) 392, 637, 837, 1226, 1518
FatI CATG 7 cut(s) 316, 796, 833, 1003, 1014, 1067, 1149
FauI CCCGC 1 cut(s) 26
FauNDI CATATG 1 cut(s) 880
FokI GGATG 4 cut(s) 356, 608, 1176, 1241
FspBI CTAG 4 cut(s) 459, 674, 1463, 1493
GlaI GCGC 1 cut(s) 580
GsuI CTGGAG 2 cut(s) 407, 1473
HaeIII GGCC 3 cut(s) 398, 435, 631
HapII CCGG 3 cut(s) 264, 812, 1034
HgaI GACGC 2 cut(s) 212, 1478
HhaI GCGC 1 cut(s) 581
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 7 cut(s) 320, 800, 837, 1007, 1018, 1071, 1153
Hin6I GCGC 1 cut(s) 579
HinP1I GCGC 1 cut(s) 579
HindIII AAGCTT 2 cut(s) 1550, 1571
HinfI GANTC 7 cut(s) 134, 179, 259, 386, 1040, 1239, 1473
HpaII CCGG 3 cut(s) 264, 812, 1034
HphI GGTGA 5 cut(s) 118, 514, 935, 962, 1244
Hpy188III TCNNGA 3 cut(s) 1034, 1335, 1452
Hpy99I CGWCG 6 cut(s) 161, 179, 191, 194, 206, 212
HpyAV CCTTC 7 cut(s) 115, 283, 325, 364, 368, 1219, 1491
HpyCH4III ACNGT 4 cut(s) 705, 892, 997, 1406
HpyF10VI GCNNNNNNNGC 8 cut(s) 30, 39, 415, 418, 441, 444, 1162, 1337
HpyF3I CTNAG 6 cut(s) 43, 240, 278, 499, 570, 1521
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 7 cut(s) 320, 800, 837, 1007, 1018, 1071, 1153
HspAI GCGC 1 cut(s) 579
Kpn2I TCCGGA 1 cut(s) 1033
Kzo9I GATC 6 cut(s) 495, 667, 757, 883, 1006, 1290
LmnI GCTCC 5 cut(s) 52, 80, 426, 452, 1337
Lsp1109I GCAGC 7 cut(s) 421, 424, 427, 430, 1140, 1189, 1504
LweI GCATC 3 cut(s) 517, 575, 1340
MaeI CTAG 4 cut(s) 459, 674, 1463, 1493
MaeIII GTNAC 7 cut(s) 520, 635, 815, 941, 1250, 1300, 1319
MalI GATC 6 cut(s) 497, 669, 759, 885, 1008, 1292
MbiI CCGCTC 1 cut(s) 447
MboI GATC 6 cut(s) 495, 667, 757, 883, 1006, 1290
MboII GAAGA 8 cut(s) 72, 125, 283, 313, 337, 340, 347, 923
MflI RGATCY 2 cut(s) 495, 667
MhlI GDGCHC 1 cut(s) 1063
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 2 cut(s) 1106, 1429
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 6 cut(s) 188, 253, 380, 1049, 1248, 1482
MmeI TCCRAC 4 cut(s) 210, 979, 1399, 1456
Mox20I TGGCCA 1 cut(s) 398
Mph1103I ATGCAT 1 cut(s) 1175
MroI TCCGGA 1 cut(s) 1033
MscI TGGCCA 1 cut(s) 398
MseI TTAA 5 cut(s) 839, 894, 1179, 1428, 1548
MslI CAYNNNNRTG 5 cut(s) 527, 594, 948, 1078, 1212
Msp20I TGGCCA 1 cut(s) 398
MspA1I CMGCKG 1 cut(s) 432
MspCI CTTAAG 1 cut(s) 1547
MspI CCGG 3 cut(s) 264, 812, 1034
MspR9I CCNGG 4 cut(s) 264, 265, 425, 813
Mva1269I GAATGC 2 cut(s) 910, 1189
MvaI CCWGG 1 cut(s) 425
MvnI CGCG 3 cut(s) 581, 1045, 1489
MwoI GCNNNNNNNGC 8 cut(s) 30, 39, 415, 418, 441, 444, 1162, 1337
NciI CCSGG 3 cut(s) 264, 265, 813
NdeI CATATG 1 cut(s) 880
NdeII GATC 6 cut(s) 495, 667, 757, 883, 1006, 1290
NlaIII CATG 7 cut(s) 320, 800, 837, 1007, 1018, 1071, 1153
NlaIV GGNNCC 4 cut(s) 669, 825, 1032, 1485
NmuCI GTSAC 7 cut(s) 520, 635, 815, 941, 1250, 1300, 1319
NotI GCGGCCGC 1 cut(s) 433
NsiI ATGCAT 1 cut(s) 1175
NspI RCATGY 2 cut(s) 800, 1071
OliI CACNNNNGTG 2 cut(s) 527, 948
PaeI GCATGC 1 cut(s) 1071
PcsI WCGNNNNNNNCGW 4 cut(s) 183, 198, 216, 345
PctI GAATGC 2 cut(s) 910, 1189
PfeI GAWTC 1 cut(s) 134
PflFI GACNNNGTC 1 cut(s) 206
PfoI TCCNGGA 1 cut(s) 423
PleI GAGTC 6 cut(s) 187, 253, 380, 1048, 1247, 1481
PpsI GAGTC 6 cut(s) 187, 253, 380, 1048, 1247, 1481
PpuMI RGGWCCY 1 cut(s) 824
Psp5II RGGWCCY 1 cut(s) 824
Psp6I CCWGG 1 cut(s) 423
PspEI GGTNACC 1 cut(s) 941
PspGI CCWGG 1 cut(s) 423
PspN4I GGNNCC 4 cut(s) 669, 825, 1032, 1485
PspPI GGNCC 3 cut(s) 630, 824, 1146
PspPPI RGGWCCY 1 cut(s) 824
PstNI CAGNNNCTG 1 cut(s) 1340
PsuI RGATCY 2 cut(s) 495, 667
PsyI GACNNNGTC 1 cut(s) 206
RsaI GTAC 1 cut(s) 787
RsaNI GTAC 1 cut(s) 786
RseI CAYNNNNRTG 5 cut(s) 527, 594, 948, 1078, 1212
SaqAI TTAA 5 cut(s) 839, 894, 1179, 1428, 1548
Sau3AI GATC 6 cut(s) 495, 667, 757, 883, 1006, 1290
Sau96I GGNCC 3 cut(s) 630, 824, 1146
SchI GAGTC 6 cut(s) 188, 253, 380, 1049, 1248, 1482
ScrFI CCNGG 4 cut(s) 264, 265, 425, 813
SduI GDGCHC 1 cut(s) 1063
SfaNI GCATC 3 cut(s) 517, 575, 1340
SinI GGWCC 2 cut(s) 824, 1146
SmaI CCCGGG 1 cut(s) 265
SmiMI CAYNNNNRTG 5 cut(s) 527, 594, 948, 1078, 1212
SmlI CTYRAG 1 cut(s) 1547
SmoI CTYRAG 1 cut(s) 1547
SphI GCATGC 1 cut(s) 1071
Sse9I AATT 2 cut(s) 1106, 1429
SspMI CTAG 4 cut(s) 459, 674, 1463, 1493
StyD4I CCNGG 4 cut(s) 262, 263, 423, 811
StyI CCWWGG 3 cut(s) 51, 399, 946
TaaI ACNGT 4 cut(s) 705, 892, 997, 1406
TaqI TCGA 3 cut(s) 886, 1009, 1312
TaqII GACCGA 1 cut(s) 210
TasI AATT 2 cut(s) 1106, 1429
TauI GCSGC 9 cut(s) 27, 30, 33, 148, 239, 435, 438, 447, 1331
TfiI GAWTC 1 cut(s) 134
Tru1I TTAA 5 cut(s) 839, 894, 1179, 1428, 1548
Tru9I TTAA 5 cut(s) 839, 894, 1179, 1428, 1548
TscAI CASTG 4 cut(s) 534, 632, 640, 1324
TseFI GTSAC 7 cut(s) 520, 635, 815, 941, 1250, 1300, 1319
TseI GCWGC 7 cut(s) 409, 412, 415, 418, 1153, 1202, 1517
Tsp45I GTSAC 7 cut(s) 520, 635, 815, 941, 1250, 1300, 1319
TspDTI ATGAA 5 cut(s) 17, 758, 867, 1119, 1263
TspGWI ACGGA 2 cut(s) 991, 1046
TspMI CCCGGG 1 cut(s) 263
TspRI CASTG 4 cut(s) 534, 632, 640, 1324
Tth111I GACNNNGTC 1 cut(s) 206
Vha464I CTTAAG 1 cut(s) 1547
VpaK11BI GGWCC 2 cut(s) 824, 1146
XapI RAATTY 1 cut(s) 1106
XceI RCATGY 2 cut(s) 800, 1071
XmaI CCCGGG 1 cut(s) 263
XspI CTAG 4 cut(s) 459, 674, 1463, 1493
Zsp2I ATGCAT 1 cut(s) 1175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.