Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0001291

Physical Location & Seq

Genomic Coordinates Forward (+)

63241866 .. 63244810

Gene Structure 2945 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030540

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2514 bp

ATGGCCGAGACGACATTCAATATCATGTATAGAAACAAAACCTCTTTAAATGTGTGTTATAACAAAACAGAAACCAGTAACACCGGGCTATGGAGTGCAGAAAACCCCTTGGTATCATCTCTCCCAATGCTCATCATCCAACTGCTCGCCGTCTTGTTGCTCGGTCACATTCTTGCCTACCTCCTCAAACCCTTGCGCCAACCACGCATTGTTTCTAACATTCTTGCTGGCGTTCTATTAGGTCCATCTTTCTTTGCTATCACGAGAATCGTGGGAGTTTCCATACTTCCTTTCGATAGCATAATGACCTTGGAGACTCTGGCAAACTTGGGGCTCGTGTACCACATTTTCCTCATTGGGTTAGAGTTTGAATTCAGACCAGTAGTACGTGCAGGGACAAAGGCTCTCAGCATTGCCCTCGCCGGCGTGGTCTTTGCCATCCCCGCCGGGTGGGCATTGCATACCTATGTACTCTTCCGCGACTTTGAAACCGTGAAAGCCAAAAGCAATATGAAAAATGTAGCGTACGGCCCCATCTTCTGGGGCATTGTCCTCTCCAGCACCAACTTCGCTGATCTCACCCAAATTCTTGCCGACTTCAAGCTTCTCCACTCGGAGGTGGGACGACTAGCCTTATCCGGTGCCATTATCTCCGATGTGGCGTCATGGTTTCTCTTTCTTGTGGTAATGGCCATACTCGGTTATGGTAAATTGTACACGGTGTTGTTAACGTTAGGCTTCATTGCATTCAGTATTTTTTTACTTCGGCCAGCTCTCGCCTGGGTAATGCGCCACACCACCAAGGAAGACGGCTATAGTGATGTCCAAATATGCTTCATTCTGTTTGGGATTGTATTGAGTGGATTAATAACCGATGCCTGCGGGGGACACTCCATCGTCGGACCTTTCATGTTGGGAGCAATTATGCCCAGGGGGATACACTTCAAACAAATGTTTGCAGATCGTGTTGGAAAATTTGTGGAAAGCCTGATGATGCCCCTCTTTTACTTGGTCATTGGGGCGAGATTCCATTCTTTTTGGCTACTCGGTGATCTAAATAACAATCTGCAAGCTGAATCCACCGTCCCTCAGCTTGTTGGGGTGATCATCCTGGCTTTCACAGCTAAGAGTGTCAGCACTTTCATCGCTGCTCTACTCAACAAAATGACACCTCGAGATGGTTTAGTTCTGGGAATCCTTATGAACACTAAAGGCATATTGGCACTCCTTATACTCACCTCCGCTCGTGACCTAAAGGCTTTGGACACCCAAACATATGGAGTAATGTTGGTTGCAGTTTGGGCAATGACGGTTCCTGTTGGACCCTTCCTAGCCCTCATTTGCAAGTCCACCAAGAGTTCTACACAACATAAACAGAGAAGCATACAAAGTGTAGGAGTTGAAACAGAGCTTCGAATCCTTGCATGCACCCATACTGCGAGCAACGCATCAGGCATCATAAGCCTCATTGACGCTTCCAATCCATCCAGCAAATCCCCAATTCATGTCTTTGCTGTCAAACTGGTGGAACTTACCGACCATAATGCTGCTATGCTCATCGTGCATGATGCTTGCAACAAAACTGGAGCAGATGGCAACGACGACTTGAGCTCCAATGCATTTGAGAACTATGCAAGGCAAAGAGAAAATGTCTCCGTTCAATCACTCACCGCAGTGTCTGCCTATGCCACTATGCACGAGGATATATGCAACATGGCTGACGAGAAGCACGTCAACTTCATAATCCTCCCGTTCCGTATGCCAACTACTATTGATGGAGTCCCAGATGATCGTTATGATCCTCTTCTCACGGAAATTAGCATGAAGGTCATGGAAAATGCAAAGTGCTCTGTAGGTCTTTTGGTGGACTGTGGGCTTGTCACATTCCATAACGCTTCTTTGGAGTCCCAAAACTTCGTCATGTTTTTCATTGGAGGGCCAGATGATCGAGAGGCACTAGCGTACGCATGGAGGATGGCTAGGCATCCTAGGGTAAACTTAAAAGTTATACGGTTCACCCTCAGTAAAGAGGCATGGGAGGTGAGTTATCATCACGATGGCAATGAGGAATACAAGACAAATGAAAACATTAGGCAGCAGAAACAACCAGACGACGCATGCATAGATGAGTTCAAACTTAAGTCCATGAATGAACCCTCAATAGTTCTGTGTGAGAAGCTGGTGAATAATTTGGAAGACATTATCAATATAGTAAGCACCATGAAAGGTCAATATCAGCTGTACATAGTGGGAAGAGGTCATGATGGTTGCTCCCCAATAACAGCACCGTTGTTCGAATGCAACAAGACCAATAAGCTCGGAACTTGGGGGGAAACATTGGCATCTTCAAGCGTCATGGGTAGTACATCCCTTCTTATAATACAGCAGGGTGCCTCTGTGGATGAACTAGATGAGAACATGGGATCTGACGGGAAACATTTTGGGGATACAACTCGGCAACCTCCCGTAAAAGACGCTGAAGATTCCTTGCTTAGAACTTCATCAGTAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

838

Amino Acids

91.97

Weight (kDa)

6.17

Isoelectric Point (pI)

34.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	55 - 443	3.8e-49	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	503 - 628	5.2e-09	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	641 - 796	2.1e-09	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001291)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g25710 FvH4_5g31470 FvH4_7g01800
malus_domestica	MD02G1284400.v1.1 MD02G1284600.v1.1 MD02G1284900.v1.1 MD02G1285000.v1.1 MD02G1285100.v1.1 MD02G1296900.v1.1 MD07G1050200.v1.1 MD07G1050300.v1.1 MD15G1139600.v1.1
prunus_persica	Prupe.2G027500_v2.0.a1 Prupe.2G038300_v2.0.a1 Prupe.2G040900_v2.0.a1 Prupe.4G283300_v2.0.a1 Prupe.4G283400_v2.0.a1 Prupe.4G283500_v2.0.a1 Prupe.4G283500_v2.0.a1 Prupe.4G284600_v2.0.a1 Prupe.4G284900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0318411 RchiOBHm_Chr5g0030741 RchiOBHm_Chr7g0226891
rosa_laevigata	RLG00000001685 RLG00000030540 RLG00000033248
rosa_multiflora	Rmu_co8328645.1_g000001 Rmu_co8411059.1_g000001 Rmu_sc0002662.1_g000016 Rmu_sc0003238.1_g000035 Rmu_sc0003936.1_g000039 Rmu_sc0034904.1_g000001
rosa_roxburghii	Rroxscaffold_3G00232440 Rroxscaffold_4G00329260
rosa_rugosa	Rorug01G0021100 Rorug05G0120500 Rorug07G0240000.1
rosa_samantha	Rh1AG033200 Rh1BG029000 Rh1CG031500 Rh1DG038000 Rh5AG212000 Rh5BG211100 Rh5CG234800 Rh5DG216300 Rh7AG387600 Rh7BG374100 Rh7CG407200 Rh7DG386300
rosa_wichuraiana	Rw0G000940 Rw0G000950 Rw1G002660 Rw5G019460 Rw7G032560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	60, 2378
AccB1I	GGYRCC	2 cut(s)	641, 2390
AccB7I	CCANNNNNTGG	1 cut(s)	540
AccBSI	CCGCTC	1 cut(s)	1244
AccII	CGCG	1 cut(s)	480
AciI	CCGC	5 cut(s)	444, 478, 882, 1242, 1671
AclI	AACGTT	1 cut(s)	731
AclWI	GGATC	2 cut(s)	1793, 2431
AcoI	YGGCCR	3 cut(s)	3, 690, 767
AcsI	RAATTY	3 cut(s)	371, 585, 974
AcuI	CTGAAG	1 cut(s)	2499
AcyI	GRCGYC	1 cut(s)	662
AfaI	GTAC	8 cut(s)	341, 387, 471, 527, 716, 1964, 2243, 2365
AfiI	CCNNNNNNNGG	5 cut(s)	90, 450, 540, 616, 1178
AflII	CTTAAG	1 cut(s)	2138
AgsI	TTSAA	9 cut(s)	19, 371, 488, 601, 946, 1403, 1661, 2134, 2349
AjiI	CACGTC	1 cut(s)	1732
AjnI	CCWGG	3 cut(s)	779, 929, 1110
AleI	CACNNNNGTG	1 cut(s)	1673
Alw21I	GWGCWC	2 cut(s)	1613, 1850
Alw26I	GTCTC	3 cut(s)	2, 308, 1657
AlwI	GGATC	2 cut(s)	1793, 2431
AlwNI	CAGNNNCTG	1 cut(s)	1679
Ama87I	CYCGRG	1 cut(s)	1173
AoxI	GGCC	5 cut(s)	3, 529, 690, 767, 1937
ApeKI	GCWGC	3 cut(s)	1148, 1547, 2095
ApoI	RAATTY	3 cut(s)	371, 585, 974
AseI	ATTAAT	1 cut(s)	866
AspA2I	CCTAGG	1 cut(s)	1988
AspLEI	GCGC	2 cut(s)	198, 792
AspS9I	GGNCC	5 cut(s)	242, 530, 902, 1322, 1937
AsuC2I	CCSGG	2 cut(s)	85, 448
AsuHPI	GGTGA	8 cut(s)	571, 1061, 1114, 1228, 1660, 2008, 2053, 2194
AsuII	TTCGAA	2 cut(s)	1414, 2295
AvaI	CYCGRG	1 cut(s)	1173
AvaII	GGWCC	3 cut(s)	242, 902, 1322
AvrII	CCTAGG	1 cut(s)	1988
BalI	TGGCCA	1 cut(s)	692
BanI	GGYRCC	2 cut(s)	641, 2390
BanII	GRGCYC	2 cut(s)	336, 1613
BauI	CACGAG	4 cut(s)	262, 335, 1245, 1697
BbsI	GAAGAC	2 cut(s)	813, 2202
Bbv12I	GWGCWC	2 cut(s)	1613, 1850
BbvCI	CCTCAGC	1 cut(s)	1089
BbvI	GCAGC	3 cut(s)	1135, 1534, 2107
BceAI	ACGGC	3 cut(s)	134, 544, 826
BcgI	CGANNNNNNTGC	8 cut(s)	400, 434, 550, 584, 1126, 1160, 1526, 1560
BciT130I	CCWGG	3 cut(s)	781, 931, 1112
BciVI	GTATCC	2 cut(s)	930, 2440
BclI	TGATCA	1 cut(s)	1104
BcnI	CCSGG	2 cut(s)	85, 448
BcoDI	GTCTC	3 cut(s)	2, 308, 1657
BfaI	CTAG	7 cut(s)	629, 1331, 1958, 1980, 1989, 2408, 2512
BfmI	CTRYAG	2 cut(s)	814, 1851
BfrI	CTTAAG	1 cut(s)	2138
BfuI	GTATCC	2 cut(s)	930, 2440
BglI	GCCNNNNNGGC	1 cut(s)	452
BisI	GCNGC	3 cut(s)	1149, 1548, 2096
BlnI	CCTAGG	1 cut(s)	1988
BlsI	GCNGC	3 cut(s)	1150, 1549, 2097
Bme1390I	CCNGG	5 cut(s)	85, 448, 781, 931, 1112
Bme18I	GGWCC	3 cut(s)	242, 902, 1322
BmeT110I	CYCGRG	1 cut(s)	1173
BmgBI	CACGTC	1 cut(s)	1732
BmgT120I	GGNCC	5 cut(s)	242, 530, 902, 1322, 1937
BmiI	GGNNCC	5 cut(s)	532, 643, 1314, 1324, 2392
BmrFI	CCNGG	5 cut(s)	85, 448, 781, 931, 1112
BmsI	GCATC	7 cut(s)	865, 984, 1457, 1464, 1558, 1993, 2351
BpiI	GAAGAC	2 cut(s)	813, 2202
BpmI	CTGGAG	2 cut(s)	541, 1605
Bpu10I	CCTNAGC	1 cut(s)	1089
Bpu14I	TTCGAA	2 cut(s)	1414, 2295
BpuEI	CTTGAG	1 cut(s)	1627
BpuMI	CCSGG	2 cut(s)	85, 448
BsaAI	YACGTR	1 cut(s)	389
BsaHI	GRCGYC	1 cut(s)	662
BsaJI	CCNNGG	7 cut(s)	108, 309, 780, 801, 929, 930, 1988
BsaWI	WCCGGW	1 cut(s)	638
BsaXI	ACNNNNNCTCC	2 cut(s)	1595, 1625
Bsc4I	CCNNNNNNNGG	5 cut(s)	90, 450, 540, 616, 1178
Bse118I	RCCGGY	1 cut(s)	422
Bse1I	ACTGG	4 cut(s)	75, 380, 1527, 1588
Bse3DI	GCAATG	5 cut(s)	411, 455, 741, 1311, 2068
BseBI	CCWGG	3 cut(s)	781, 931, 1112
BseDI	CCNNGG	7 cut(s)	108, 309, 780, 801, 929, 930, 1988
BseGI	GGATG	8 cut(s)	135, 438, 1107, 1484, 1980, 1984, 2366, 2407
BseLI	CCNNNNNNNGG	5 cut(s)	90, 450, 540, 616, 1178
BseMI	GCAATG	5 cut(s)	411, 455, 741, 1311, 2068
BseMII	CTCAG	3 cut(s)	421, 1103, 2035
BseNI	ACTGG	4 cut(s)	75, 380, 1527, 1588
BseRI	GAGGAG	1 cut(s)	173
BseXI	GCAGC	3 cut(s)	1135, 1534, 2107
BsgI	GTGCAG	2 cut(s)	117, 411
Bsh1236I	CGCG	1 cut(s)	480
BshFI	GGCC	5 cut(s)	5, 531, 692, 769, 1939
BshNI	GGYRCC	2 cut(s)	641, 2390
BsiHKAI	GWGCWC	2 cut(s)	1613, 1850
BsiHKCI	CYCGRG	1 cut(s)	1173
BsiSI	CCGG	4 cut(s)	84, 423, 447, 639
BsiWI	CGTACG	2 cut(s)	525, 1962
BslFI	GGGAC	6 cut(s)	409, 636, 900, 1070, 1766, 1891
BslI	CCNNNNNNNGG	5 cut(s)	90, 450, 540, 616, 1178
BsmAI	GTCTC	3 cut(s)	2, 308, 1657
BsmBI	CGTCTC	1 cut(s)	2
BsmFI	GGGAC	6 cut(s)	409, 636, 900, 1070, 1766, 1891
BsmI	GAATGC	2 cut(s)	746, 2303
BsnI	GGCC	5 cut(s)	5, 531, 692, 769, 1939
BsoBI	CYCGRG	1 cut(s)	1173
Bsp119I	TTCGAA	2 cut(s)	1414, 2295
Bsp1286I	GDGCHC	3 cut(s)	336, 1613, 1850
Bsp1407I	TGTACA	2 cut(s)	714, 2241
Bsp143I	GATC	8 cut(s)	574, 961, 1051, 1104, 1789, 1798, 1945, 2423
BspACI	CCGC	5 cut(s)	444, 478, 882, 1242, 1671
BspANI	GGCC	5 cut(s)	5, 531, 692, 769, 1939
BspCNI	CTCAG	3 cut(s)	420, 1102, 2034
BspFNI	CGCG	1 cut(s)	480
BspHI	TCATGA	1 cut(s)	2260
BspLI	GGNNCC	5 cut(s)	532, 643, 1314, 1324, 2392
BspPI	GGATC	2 cut(s)	1793, 2431
BspT104I	TTCGAA	2 cut(s)	1414, 2295
BspT107I	GGYRCC	2 cut(s)	641, 2390
BspTI	CTTAAG	1 cut(s)	2138
BsrBI	CCGCTC	1 cut(s)	1244
BsrDI	GCAATG	5 cut(s)	411, 455, 741, 1311, 2068
BsrFI	RCCGGY	1 cut(s)	422
BsrGI	TGTACA	2 cut(s)	714, 2241
BsrI	ACTGG	4 cut(s)	75, 380, 1527, 1588
BssAI	RCCGGY	1 cut(s)	422
BssECI	CCNNGG	7 cut(s)	108, 309, 780, 801, 929, 930, 1988
BssMI	GATC	8 cut(s)	574, 961, 1051, 1104, 1789, 1798, 1945, 2423
BssNI	GRCGYC	1 cut(s)	662
BssSI	CACGAG	4 cut(s)	262, 335, 1245, 1697
BssT1I	CCWWGG	4 cut(s)	108, 309, 801, 1988
Bst2BI	CACGAG	4 cut(s)	262, 335, 1245, 1697
Bst2UI	CCWGG	3 cut(s)	781, 931, 1112
Bst4CI	ACNGT	7 cut(s)	493, 721, 1084, 1312, 1871, 2013, 2289
Bst6I	CTCTTC	3 cut(s)	479, 1809, 2248
BstACI	GRCGYC	1 cut(s)	662
BstAFI	CTTAAG	1 cut(s)	2138
BstAPI	GCANNNNNTGC	1 cut(s)	1679
BstAUI	TGTACA	2 cut(s)	714, 2241
BstBAI	YACGTR	1 cut(s)	389
BstBI	TTCGAA	2 cut(s)	1414, 2295
BstDEI	CTNAG	5 cut(s)	407, 1089, 1125, 2021, 2492
BstF5I	GGATG	8 cut(s)	135, 438, 1107, 1484, 1980, 1984, 2366, 2407
BstFNI	CGCG	1 cut(s)	480
BstHHI	GCGC	2 cut(s)	198, 792
BstKTI	GATC	8 cut(s)	577, 964, 1054, 1107, 1792, 1801, 1948, 2426
BstMAI	GTCTC	3 cut(s)	2, 308, 1657
BstMBI	GATC	8 cut(s)	574, 961, 1051, 1104, 1789, 1798, 1945, 2423
BstMWI	GCNNNNNNNGC	9 cut(s)	204, 443, 452, 1121, 1301, 1445, 1461, 1561, 1679
BstNI	CCWGG	3 cut(s)	781, 931, 1112
BstNSI	RCATGY	2 cut(s)	1428, 2121
BstSCI	CCNGG	5 cut(s)	83, 446, 779, 929, 1110
BstSFI	CTRYAG	2 cut(s)	814, 1851
BstUI	CGCG	1 cut(s)	480
BstV1I	GCAGC	3 cut(s)	1135, 1534, 2107
BstV2I	GAAGAC	2 cut(s)	813, 2202
BstX2I	RGATCY	1 cut(s)	2423
BstXI	CCANNNNNNTGG	1 cut(s)	1277
BstYI	RGATCY	1 cut(s)	2423
BsuI	GTATCC	2 cut(s)	930, 2440
BsuRI	GGCC	5 cut(s)	5, 531, 692, 769, 1939
BtgZI	GCGATG	1 cut(s)	1129
BtrI	CACGTC	1 cut(s)	1732
BtsCI	GGATG	8 cut(s)	135, 438, 1107, 1484, 1980, 1984, 2366, 2407
BtsI	GCAGTG	1 cut(s)	1680
BtsIMutI	CAGTG	1 cut(s)	1680
CaiI	CAGNNNCTG	1 cut(s)	1679
CciI	TCATGA	1 cut(s)	2260
CfoI	GCGC	2 cut(s)	198, 792
Cfr10I	RCCGGY	1 cut(s)	422
Cfr13I	GGNCC	5 cut(s)	242, 530, 902, 1322, 1937
CseI	GACGC	5 cut(s)	651, 1481, 2123, 2341, 2483
Csp6I	GTAC	8 cut(s)	340, 386, 470, 526, 715, 1963, 2242, 2364
CviQI	GTAC	8 cut(s)	340, 386, 470, 526, 715, 1963, 2242, 2364
DdeI	CTNAG	5 cut(s)	407, 1089, 1125, 2021, 2492
DpnI	GATC	8 cut(s)	576, 963, 1053, 1106, 1791, 1800, 1947, 2425
DpnII	GATC	8 cut(s)	574, 961, 1051, 1104, 1789, 1798, 1945, 2423
DraI	TTTAAA	1 cut(s)	48
EaeI	YGGCCR	3 cut(s)	3, 690, 767
Eam1104I	CTCTTC	3 cut(s)	479, 1809, 2248
EarI	CTCTTC	3 cut(s)	479, 1809, 2248
Ecl136II	GAGCTC	1 cut(s)	1611
Eco130I	CCWWGG	4 cut(s)	108, 309, 801, 1988
Eco24I	GRGCYC	2 cut(s)	336, 1613
Eco47I	GGWCC	3 cut(s)	242, 902, 1322
Eco53kI	GAGCTC	1 cut(s)	1611
Eco57I	CTGAAG	1 cut(s)	2499
Eco88I	CYCGRG	1 cut(s)	1173
EcoICRI	GAGCTC	1 cut(s)	1611
EcoRI	GAATTC	1 cut(s)	371
EcoRII	CCWGG	3 cut(s)	779, 929, 1110
EcoT14I	CCWWGG	4 cut(s)	108, 309, 801, 1988
EcoT22I	ATGCAT	2 cut(s)	1621, 2123
EcoT38I	GRGCYC	2 cut(s)	336, 1613
ErhI	CCWWGG	4 cut(s)	108, 309, 801, 1988
Esp3I	CGTCTC	1 cut(s)	2
FaqI	GGGAC	6 cut(s)	409, 636, 900, 1070, 1766, 1891
FauI	CCCGC	2 cut(s)	451, 875
FauNDI	CATATG	1 cut(s)	1276
FbaI	TGATCA	1 cut(s)	1104
Fnu4HI	GCNGC	3 cut(s)	1149, 1548, 2096
FokI	GGATG	8 cut(s)	122, 425, 1094, 1471, 1971, 1987, 2353, 2414
FriOI	GRGCYC	2 cut(s)	336, 1613
Fsp4HI	GCNGC	3 cut(s)	1149, 1548, 2096
FspBI	CTAG	7 cut(s)	629, 1331, 1958, 1980, 1989, 2408, 2512
GlaI	GCGC	2 cut(s)	197, 791
GluI	GCNGC	3 cut(s)	1149, 1548, 2096
GsuI	CTGGAG	2 cut(s)	541, 1605
HaeIII	GGCC	5 cut(s)	5, 531, 692, 769, 1939
HapII	CCGG	4 cut(s)	84, 423, 447, 639
HgaI	GACGC	5 cut(s)	651, 1481, 2123, 2341, 2483
HhaI	GCGC	2 cut(s)	198, 792
Hin1I	GRCGYC	1 cut(s)	662
Hin6I	GCGC	2 cut(s)	196, 790
HinP1I	GCGC	2 cut(s)	196, 790
HincII	GTYRAC	2 cut(s)	729, 1735
HindII	GTYRAC	2 cut(s)	729, 1735
HindIII	AAGCTT	1 cut(s)	602
HinfI	GANTC	9 cut(s)	267, 316, 1026, 1076, 1194, 1416, 1779, 1904, 2483
HpaI	GTTAAC	1 cut(s)	729
HpaII	CCGG	4 cut(s)	84, 423, 447, 639
HphI	GGTGA	8 cut(s)	571, 1061, 1114, 1228, 1660, 2008, 2053, 2194
Hpy166II	GTNNAC	9 cut(s)	340, 717, 729, 1350, 1735, 1867, 1996, 2016, 2509
Hpy188I	TCNGA	6 cut(s)	377, 616, 655, 902, 2321, 2428
Hpy188III	TCNNGA	6 cut(s)	262, 1175, 1247, 1949, 2054, 2261
Hpy8I	GTNNAC	9 cut(s)	340, 717, 729, 1350, 1735, 1867, 1996, 2016, 2509
Hpy99I	CGWCG	3 cut(s)	902, 1604, 2117
HpyAV	CCTTC	3 cut(s)	1336, 1819, 2381
HpyCH4III	ACNGT	7 cut(s)	493, 721, 1084, 1312, 1871, 2013, 2289
HpyCH4IV	ACGT	3 cut(s)	388, 731, 1731
HpyF10VI	GCNNNNNNNGC	9 cut(s)	204, 443, 452, 1121, 1301, 1445, 1461, 1561, 1679
HpyF3I	CTNAG	5 cut(s)	407, 1089, 1125, 2021, 2492
HpySE526I	ACGT	3 cut(s)	388, 731, 1731
Hsp92I	GRCGYC	1 cut(s)	662
HspAI	GCGC	2 cut(s)	196, 790
KroI	GCCGGC	1 cut(s)	422
KroNI	GCCGGC	1 cut(s)	424
Ksp22I	TGATCA	1 cut(s)	1104
KspAI	GTTAAC	1 cut(s)	729
Kzo9I	GATC	8 cut(s)	574, 961, 1051, 1104, 1789, 1798, 1945, 2423
LmnI	GCTCC	4 cut(s)	917, 1586, 1616, 2276
Lsp1109I	GCAGC	3 cut(s)	1135, 1534, 2107
LweI	GCATC	7 cut(s)	865, 984, 1457, 1464, 1558, 1993, 2351
MaeI	CTAG	7 cut(s)	629, 1331, 1958, 1980, 1989, 2408, 2512
MaeII	ACGT	3 cut(s)	388, 731, 1731
MaeIII	GTNAC	4 cut(s)	77, 164, 1247, 1879
MalI	GATC	8 cut(s)	576, 963, 1053, 1106, 1791, 1800, 1947, 2425
MbiI	CCGCTC	1 cut(s)	1244
MboI	GATC	8 cut(s)	574, 961, 1051, 1104, 1789, 1798, 1945, 2423
MboII	GAAGA	8 cut(s)	466, 529, 818, 1796, 2207, 2265, 2337, 2492
MflI	RGATCY	1 cut(s)	2423
MhlI	GDGCHC	3 cut(s)	336, 1613, 1850
MlsI	TGGCCA	1 cut(s)	692
MluCI	AATT	8 cut(s)	371, 585, 710, 921, 974, 1500, 1815, 2188
MluNI	TGGCCA	1 cut(s)	692
MlyI	GAGTC	3 cut(s)	310, 1788, 1913
MmeI	TCCRAC	4 cut(s)	163, 880, 949, 1300
Mox20I	TGGCCA	1 cut(s)	692
Mph1103I	ATGCAT	2 cut(s)	1621, 2123
MreI	CGCCGGCG	1 cut(s)	422
MroNI	GCCGGC	1 cut(s)	422
MscI	TGGCCA	1 cut(s)	692
MseI	TTAA	5 cut(s)	47, 728, 866, 2000, 2139
MslI	CAYNNNNRTG	3 cut(s)	465, 1673, 2055
Msp20I	TGGCCA	1 cut(s)	692
MspA1I	CMGCKG	1 cut(s)	2239
MspCI	CTTAAG	1 cut(s)	2138
MspI	CCGG	4 cut(s)	84, 423, 447, 639
MspR9I	CCNGG	5 cut(s)	85, 448, 781, 931, 1112
Mva1269I	GAATGC	2 cut(s)	746, 2303
MvaI	CCWGG	3 cut(s)	781, 931, 1112
MvnI	CGCG	1 cut(s)	480
MwoI	GCNNNNNNNGC	9 cut(s)	204, 443, 452, 1121, 1301, 1445, 1461, 1561, 1679
NaeI	GCCGGC	1 cut(s)	424
NciI	CCSGG	2 cut(s)	85, 448
NdeI	CATATG	1 cut(s)	1276
NdeII	GATC	8 cut(s)	574, 961, 1051, 1104, 1789, 1798, 1945, 2423
NgoMIV	GCCGGC	1 cut(s)	422
NlaIV	GGNNCC	5 cut(s)	532, 643, 1314, 1324, 2392
NmeAIII	GCCGAG	2 cut(s)	31, 2434
NmuCI	GTSAC	3 cut(s)	164, 1247, 1879
NsiI	ATGCAT	2 cut(s)	1621, 2123
NspI	RCATGY	2 cut(s)	1428, 2121
NspV	TTCGAA	2 cut(s)	1414, 2295
OliI	CACNNNNGTG	1 cut(s)	1673
PaeI	GCATGC	2 cut(s)	1428, 2121
PaeR7I	CTCGAG	1 cut(s)	1173
PagI	TCATGA	1 cut(s)	2260
PasI	CCCWGGG	1 cut(s)	930
PcsI	WCGNNNNNNNCGW	1 cut(s)	1728
PctI	GAATGC	2 cut(s)	746, 2303
PdiI	GCCGGC	1 cut(s)	424
PfeI	GAWTC	6 cut(s)	267, 1026, 1076, 1194, 1416, 2483
Pfl23II	CGTACG	2 cut(s)	525, 1962
PflMI	CCANNNNNTGG	1 cut(s)	540
PkrI	GCNGC	3 cut(s)	1150, 1549, 2097
PleI	GAGTC	3 cut(s)	310, 1787, 1912
PpsI	GAGTC	3 cut(s)	310, 1787, 1912
Ppu21I	YACGTR	1 cut(s)	389
PshBI	ATTAAT	1 cut(s)	866
PsiI	TTATAA	2 cut(s)	60, 2378
Psp124BI	GAGCTC	1 cut(s)	1613
Psp1406I	AACGTT	1 cut(s)	731
Psp6I	CCWGG	3 cut(s)	779, 929, 1110
PspGI	CCWGG	3 cut(s)	779, 929, 1110
PspLI	CGTACG	2 cut(s)	525, 1962
PspN4I	GGNNCC	5 cut(s)	532, 643, 1314, 1324, 2392
PspPI	GGNCC	5 cut(s)	242, 530, 902, 1322, 1937
PstNI	CAGNNNCTG	1 cut(s)	1679
PsuI	RGATCY	1 cut(s)	2423
PvuII	CAGCTG	1 cut(s)	2239
RsaI	GTAC	8 cut(s)	341, 387, 471, 527, 716, 1964, 2243, 2365
RsaNI	GTAC	8 cut(s)	340, 386, 470, 526, 715, 1963, 2242, 2364
RseI	CAYNNNNRTG	3 cut(s)	465, 1673, 2055
SacI	GAGCTC	1 cut(s)	1613
SaqAI	TTAA	5 cut(s)	47, 728, 866, 2000, 2139
SatI	GCNGC	3 cut(s)	1149, 1548, 2096
Sau3AI	GATC	8 cut(s)	574, 961, 1051, 1104, 1789, 1798, 1945, 2423
Sau96I	GGNCC	5 cut(s)	242, 530, 902, 1322, 1937
SchI	GAGTC	3 cut(s)	310, 1788, 1913
ScrFI	CCNGG	5 cut(s)	85, 448, 781, 931, 1112
SduI	GDGCHC	3 cut(s)	336, 1613, 1850
SfaNI	GCATC	7 cut(s)	865, 984, 1457, 1464, 1558, 1993, 2351
SfcI	CTRYAG	2 cut(s)	814, 1851
Sfr274I	CTCGAG	1 cut(s)	1173
SfuI	TTCGAA	2 cut(s)	1414, 2295
SgrAI	CRCCGGYG	1 cut(s)	422
SinI	GGWCC	3 cut(s)	242, 902, 1322
SlaI	CTCGAG	1 cut(s)	1173
SmiMI	CAYNNNNRTG	3 cut(s)	465, 1673, 2055
SmlI	CTYRAG	3 cut(s)	1173, 1606, 2138
SmoI	CTYRAG	3 cut(s)	1173, 1606, 2138
SphI	GCATGC	2 cut(s)	1428, 2121
Sse9I	AATT	8 cut(s)	371, 585, 710, 921, 974, 1500, 1815, 2188
SsiI	CCGC	5 cut(s)	444, 478, 882, 1242, 1671
SspMI	CTAG	7 cut(s)	629, 1331, 1958, 1980, 1989, 2408, 2512
SstI	GAGCTC	1 cut(s)	1613
StyD4I	CCNGG	5 cut(s)	83, 446, 779, 929, 1110
StyI	CCWWGG	4 cut(s)	108, 309, 801, 1988
TaaI	ACNGT	7 cut(s)	493, 721, 1084, 1312, 1871, 2013, 2289
TaiI	ACGT	3 cut(s)	391, 734, 1734
TaqI	TCGA	5 cut(s)	294, 1174, 1414, 1948, 2295
TaqII	GACCGA	1 cut(s)	152
TasI	AATT	8 cut(s)	371, 585, 710, 921, 974, 1500, 1815, 2188
TatI	WGTACW	4 cut(s)	469, 714, 2241, 2363
TfiI	GAWTC	6 cut(s)	267, 1026, 1076, 1194, 1416, 2483
Tru1I	TTAA	5 cut(s)	47, 728, 866, 2000, 2139
Tru9I	TTAA	5 cut(s)	47, 728, 866, 2000, 2139
TscAI	CASTG	1 cut(s)	1680
TseFI	GTSAC	3 cut(s)	164, 1247, 1879
TseI	GCWGC	3 cut(s)	1148, 1547, 2095
Tsp45I	GTSAC	3 cut(s)	164, 1247, 1879
TspGWI	ACGGA	3 cut(s)	1645, 1745, 1826
TspRI	CASTG	1 cut(s)	1680
Van91I	CCANNNNNTGG	1 cut(s)	540
Vha464I	CTTAAG	1 cut(s)	2138
VpaK11BI	GGWCC	3 cut(s)	242, 902, 1322
VspI	ATTAAT	1 cut(s)	866
XapI	RAATTY	3 cut(s)	371, 585, 974
XceI	RCATGY	2 cut(s)	1428, 2121
XcmI	CCANNNNNNNNNTGG	1 cut(s)	777
XhoI	CTCGAG	1 cut(s)	1173
XmaJI	CCTAGG	1 cut(s)	1988
XspI	CTAG	7 cut(s)	629, 1331, 1958, 1980, 1989, 2408, 2512
Zsp2I	ATGCAT	2 cut(s)	1621, 2123

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.