Cation H( ) antiporter
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 72, 102 |
| AarI | CACCTGC | 1 cut(s) | 2299 |
| Acc36I | ACCTGC | 1 cut(s) | 2299 |
| AccB1I | GGYRCC | 1 cut(s) | 620 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 504, 1116 |
| AciI | CCGC | 8 cut(s) | 775, 835, 859, 1233, 1536, 1632, 1823, 2047 |
| AclI | AACGTT | 1 cut(s) | 20 |
| AclWI | GGATC | 5 cut(s) | 850, 1042, 1476, 1489, 1579 |
| AcoI | YGGCCR | 2 cut(s) | 489, 1621 |
| AcsI | RAATTY | 2 cut(s) | 264, 306 |
| AcyI | GRCGYC | 1 cut(s) | 2340 |
| AfaI | GTAC | 5 cut(s) | 831, 970, 2252, 2375, 2458 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 504, 640, 781, 813, 984, 1116, 1765 |
| AflII | CTTAAG | 2 cut(s) | 548, 662 |
| AflIII | ACRYGT | 3 cut(s) | 324, 399, 1113 |
| AjiI | CACGTC | 2 cut(s) | 640, 2035 |
| AjnI | CCWGG | 8 cut(s) | 381, 405, 801, 1194, 1764, 2123, 2253, 2565 |
| Alw21I | GWGCWC | 3 cut(s) | 728, 909, 1026 |
| Alw26I | GTCTC | 4 cut(s) | 139, 570, 885, 1786 |
| AlwI | GGATC | 5 cut(s) | 850, 1042, 1476, 1489, 1579 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2442 |
| ApeKI | GCWGC | 6 cut(s) | 2013, 2400, 2403, 2436, 2521, 2531 |
| ApoI | RAATTY | 2 cut(s) | 264, 306 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1358 |
| AspLEI | GCGC | 1 cut(s) | 947 |
| AspS9I | GGNCC | 8 cut(s) | 287, 385, 772, 1333, 1348, 1406, 1969, 2269 |
| AsuC2I | CCSGG | 2 cut(s) | 448, 839 |
| AsuHPI | GGTGA | 5 cut(s) | 202, 1524, 1765, 2326, 2372 |
| AvaII | GGWCC | 4 cut(s) | 1348, 1406, 1969, 2269 |
| BaeGI | GKGCMC | 2 cut(s) | 610, 1390 |
| BalI | TGGCCA | 2 cut(s) | 491, 1623 |
| BamHI | GGATCC | 1 cut(s) | 1481 |
| BanI | GGYRCC | 1 cut(s) | 620 |
| BanII | GRGCYC | 3 cut(s) | 909, 1026, 1980 |
| BauI | CACGAG | 2 cut(s) | 218, 727 |
| BbsI | GAAGAC | 4 cut(s) | 166, 1585, 2392, 2472 |
| Bbv12I | GWGCWC | 3 cut(s) | 728, 909, 1026 |
| BbvI | GCAGC | 6 cut(s) | 2025, 2412, 2415, 2448, 2518, 2533 |
| BccI | CCATC | 4 cut(s) | 500, 1784, 1869, 2252 |
| BceAI | ACGGC | 1 cut(s) | 1523 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 184, 218, 454, 488, 1018, 1052 |
| BciT130I | CCWGG | 8 cut(s) | 383, 407, 803, 1196, 1766, 2125, 2255, 2567 |
| BcnI | CCSGG | 2 cut(s) | 448, 839 |
| BcoDI | GTCTC | 4 cut(s) | 139, 570, 885, 1786 |
| BfaI | CTAG | 8 cut(s) | 210, 389, 752, 1415, 1877, 1964, 2453, 2484 |
| BfmI | CTRYAG | 3 cut(s) | 372, 1420, 2535 |
| BfrI | CTTAAG | 2 cut(s) | 548, 662 |
| BfuAI | ACCTGC | 1 cut(s) | 2299 |
| BglII | AGATCT | 2 cut(s) | 658, 1992 |
| BisI | GCNGC | 8 cut(s) | 775, 1233, 2014, 2401, 2404, 2437, 2522, 2532 |
| BlpI | GCTNAGC | 2 cut(s) | 359, 1025 |
| BlsI | GCNGC | 8 cut(s) | 776, 1234, 2015, 2402, 2405, 2438, 2523, 2533 |
| Bme18I | GGWCC | 4 cut(s) | 1348, 1406, 1969, 2269 |
| BmgBI | CACGTC | 2 cut(s) | 640, 2035 |
| BmgT120I | GGNCC | 8 cut(s) | 287, 385, 772, 1333, 1348, 1406, 1969, 2269 |
| BmiI | GGNNCC | 8 cut(s) | 288, 622, 734, 1003, 1334, 1483, 1885, 2270 |
| BmrI | ACTGGG | 2 cut(s) | 1074, 2232 |
| BmsI | GCATC | 5 cut(s) | 204, 1065, 1509, 1642, 2170 |
| BmuI | ACTGGG | 2 cut(s) | 1074, 2232 |
| BpiI | GAAGAC | 4 cut(s) | 166, 1585, 2392, 2472 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 359, 1025 |
| BpuMI | CCSGG | 2 cut(s) | 448, 839 |
| BsaAI | YACGTR | 1 cut(s) | 251 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1400 |
| BsaHI | GRCGYC | 1 cut(s) | 2340 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1817, 1847, 2273, 2303 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 504, 640, 781, 813, 984, 1116, 1765 |
| Bse118I | RCCGGY | 3 cut(s) | 433, 1217, 1325 |
| Bse1I | ACTGG | 4 cut(s) | 498, 1069, 1272, 2227 |
| Bse3DI | GCAATG | 3 cut(s) | 465, 1374, 2217 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1400 |
| BseBI | CCWGG | 8 cut(s) | 383, 407, 803, 1196, 1766, 2125, 2255, 2567 |
| BseGI | GGATG | 3 cut(s) | 1500, 1706, 2263 |
| BseJI | GATNNNNATC | 1 cut(s) | 1400 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 504, 640, 781, 813, 984, 1116, 1765 |
| BseMI | GCAATG | 3 cut(s) | 465, 1374, 2217 |
| BseMII | CTCAG | 6 cut(s) | 350, 603, 1039, 1333, 1924, 1965 |
| BseNI | ACTGG | 4 cut(s) | 498, 1069, 1272, 2227 |
| BseRI | GAGGAG | 3 cut(s) | 641, 659, 1907 |
| BseSI | GKGCMC | 2 cut(s) | 610, 1390 |
| BseXI | GCAGC | 6 cut(s) | 2025, 2412, 2415, 2448, 2518, 2533 |
| BseYI | CCCAGC | 3 cut(s) | 1363, 2114, 2322 |
| BsgI | GTGCAG | 1 cut(s) | 2540 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1404 |
| BshNI | GGYRCC | 1 cut(s) | 620 |
| BsiEI | CGRYCG | 1 cut(s) | 1404 |
| BsiHKAI | GWGCWC | 3 cut(s) | 728, 909, 1026 |
| BsiSI | CCGG | 6 cut(s) | 434, 447, 839, 1218, 1326, 1692 |
| BslFI | GGGAC | 3 cut(s) | 804, 1564, 1838 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 504, 640, 781, 813, 984, 1116, 1765 |
| BsmAI | GTCTC | 4 cut(s) | 139, 570, 885, 1786 |
| BsmBI | CGTCTC | 1 cut(s) | 1786 |
| BsmFI | GGGAC | 3 cut(s) | 804, 1564, 1838 |
| BsmI | GAATGC | 4 cut(s) | 213, 565, 1267, 2119 |
| Bsp1286I | GDGCHC | 6 cut(s) | 610, 728, 909, 1026, 1390, 1980 |
| Bsp1407I | TGTACA | 1 cut(s) | 2373 |
| Bsp143I | GATC | 8 cut(s) | 658, 855, 1047, 1371, 1401, 1481, 1584, 1992 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 359, 1025 |
| Bsp19I | CCATGG | 1 cut(s) | 1887 |
| BspACI | CCGC | 8 cut(s) | 775, 835, 859, 1233, 1536, 1632, 1823, 2047 |
| BspCNI | CTCAG | 6 cut(s) | 351, 602, 1038, 1332, 1923, 1964 |
| BspHI | TCATGA | 1 cut(s) | 1284 |
| BspLI | GGNNCC | 8 cut(s) | 288, 622, 734, 1003, 1334, 1483, 1885, 2270 |
| BspMI | ACCTGC | 1 cut(s) | 2299 |
| BspPI | GGATC | 5 cut(s) | 850, 1042, 1476, 1489, 1579 |
| BspQI | GCTCTTC | 2 cut(s) | 69, 849 |
| BspT107I | GGYRCC | 1 cut(s) | 620 |
| BspTI | CTTAAG | 2 cut(s) | 548, 662 |
| BsrDI | GCAATG | 3 cut(s) | 465, 1374, 2217 |
| BsrFI | RCCGGY | 3 cut(s) | 433, 1217, 1325 |
| BsrGI | TGTACA | 1 cut(s) | 2373 |
| BsrI | ACTGG | 4 cut(s) | 498, 1069, 1272, 2227 |
| BssAI | RCCGGY | 3 cut(s) | 433, 1217, 1325 |
| BssMI | GATC | 8 cut(s) | 658, 855, 1047, 1371, 1401, 1481, 1584, 1992 |
| BssNI | GRCGYC | 1 cut(s) | 2340 |
| BssSI | CACGAG | 2 cut(s) | 218, 727 |
| BssT1I | CCWWGG | 3 cut(s) | 1256, 1344, 1887 |
| Bst2BI | CACGAG | 2 cut(s) | 218, 727 |
| Bst2UI | CCWGG | 8 cut(s) | 383, 407, 803, 1196, 1766, 2125, 2255, 2567 |
| Bst4CI | ACNGT | 7 cut(s) | 91, 1424, 1578, 1973, 2084, 2140, 2444 |
| Bst6I | CTCTTC | 2 cut(s) | 69, 849 |
| BstACI | GRCGYC | 1 cut(s) | 2340 |
| BstAFI | CTTAAG | 2 cut(s) | 548, 662 |
| BstAUI | TGTACA | 1 cut(s) | 2373 |
| BstBAI | YACGTR | 1 cut(s) | 251 |
| BstC8I | GCNNGC | 7 cut(s) | 225, 274, 356, 1219, 1510, 1657, 2170 |
| BstDSI | CCRYGG | 3 cut(s) | 153, 1887, 1972 |
| BstF5I | GGATG | 3 cut(s) | 1500, 1706, 2263 |
| BstHHI | GCGC | 1 cut(s) | 947 |
| BstKTI | GATC | 8 cut(s) | 661, 858, 1050, 1374, 1404, 1484, 1587, 1995 |
| BstMAI | GTCTC | 4 cut(s) | 139, 570, 885, 1786 |
| BstMBI | GATC | 8 cut(s) | 658, 855, 1047, 1371, 1401, 1481, 1584, 1992 |
| BstMCI | CGRYCG | 1 cut(s) | 1404 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 360, 627, 1180, 1447, 1913, 2022, 2046, 2236 |
| BstNI | CCWGG | 8 cut(s) | 383, 407, 803, 1196, 1766, 2125, 2255, 2567 |
| BstNSI | RCATGY | 5 cut(s) | 328, 403, 1117, 1512, 2172 |
| BstSFI | CTRYAG | 3 cut(s) | 372, 1420, 2535 |
| BstSLI | GKGCMC | 2 cut(s) | 610, 1390 |
| BstV1I | GCAGC | 6 cut(s) | 2025, 2412, 2415, 2448, 2518, 2533 |
| BstV2I | GAAGAC | 4 cut(s) | 166, 1585, 2392, 2472 |
| BstX2I | RGATCY | 4 cut(s) | 658, 1047, 1481, 1992 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2299 |
| BstYI | RGATCY | 4 cut(s) | 658, 1047, 1481, 1992 |
| BtgI | CCRYGG | 3 cut(s) | 153, 1887, 1972 |
| BtgZI | GCGATG | 1 cut(s) | 478 |
| BtrI | CACGTC | 2 cut(s) | 640, 2035 |
| BtsCI | GGATG | 3 cut(s) | 1500, 1706, 2263 |
| BtsI | GCAGTG | 1 cut(s) | 2421 |
| BtsIMutI | CAGTG | 7 cut(s) | 96, 505, 1265, 1388, 1959, 2220, 2421 |
| BveI | ACCTGC | 1 cut(s) | 2299 |
| Cac8I | GCNNGC | 7 cut(s) | 225, 274, 356, 1219, 1510, 1657, 2170 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2442 |
| CciI | TCATGA | 1 cut(s) | 1284 |
| CfoI | GCGC | 1 cut(s) | 947 |
| Cfr10I | RCCGGY | 3 cut(s) | 433, 1217, 1325 |
| Cfr13I | GGNCC | 8 cut(s) | 287, 385, 772, 1333, 1348, 1406, 1969, 2269 |
| CseI | GACGC | 1 cut(s) | 2348 |
| Csp6I | GTAC | 5 cut(s) | 830, 969, 2251, 2374, 2457 |
| CviQI | GTAC | 5 cut(s) | 830, 969, 2251, 2374, 2457 |
| DpnI | GATC | 8 cut(s) | 660, 857, 1049, 1373, 1403, 1483, 1586, 1994 |
| DpnII | GATC | 8 cut(s) | 658, 855, 1047, 1371, 1401, 1481, 1584, 1992 |
| EaeI | YGGCCR | 2 cut(s) | 489, 1621 |
| Eam1104I | CTCTTC | 2 cut(s) | 69, 849 |
| EarI | CTCTTC | 2 cut(s) | 69, 849 |
| EciI | GGCGGA | 2 cut(s) | 824, 1621 |
| Ecl136II | GAGCTC | 2 cut(s) | 907, 1024 |
| Eco130I | CCWWGG | 3 cut(s) | 1256, 1344, 1887 |
| Eco147I | AGGCCT | 1 cut(s) | 1299 |
| Eco24I | GRGCYC | 3 cut(s) | 909, 1026, 1980 |
| Eco32I | GATATC | 1 cut(s) | 12 |
| Eco47I | GGWCC | 4 cut(s) | 1348, 1406, 1969, 2269 |
| Eco53kI | GAGCTC | 2 cut(s) | 907, 1024 |
| EcoICRI | GAGCTC | 2 cut(s) | 907, 1024 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1333 |
| EcoRII | CCWGG | 8 cut(s) | 381, 405, 801, 1194, 1764, 2123, 2253, 2565 |
| EcoRV | GATATC | 1 cut(s) | 12 |
| EcoT14I | CCWWGG | 3 cut(s) | 1256, 1344, 1887 |
| EcoT22I | ATGCAT | 1 cut(s) | 1803 |
| EcoT38I | GRGCYC | 3 cut(s) | 909, 1026, 1980 |
| ErhI | CCWWGG | 3 cut(s) | 1256, 1344, 1887 |
| Esp3I | CGTCTC | 1 cut(s) | 1786 |
| FaqI | GGGAC | 3 cut(s) | 804, 1564, 1838 |
| FauI | CCCGC | 1 cut(s) | 1543 |
| FauNDI | CATATG | 1 cut(s) | 1811 |
| Fnu4HI | GCNGC | 8 cut(s) | 775, 1233, 2014, 2401, 2404, 2437, 2522, 2532 |
| FokI | GGATG | 3 cut(s) | 1487, 1693, 2270 |
| FriOI | GRGCYC | 3 cut(s) | 909, 1026, 1980 |
| Fsp4HI | GCNGC | 8 cut(s) | 775, 1233, 2014, 2401, 2404, 2437, 2522, 2532 |
| FspBI | CTAG | 8 cut(s) | 210, 389, 752, 1415, 1877, 1964, 2453, 2484 |
| GlaI | GCGC | 1 cut(s) | 946 |
| GluI | GCNGC | 8 cut(s) | 775, 1233, 2014, 2401, 2404, 2437, 2522, 2532 |
| GsaI | CCCAGC | 3 cut(s) | 1367, 2118, 2326 |
| HapII | CCGG | 6 cut(s) | 434, 447, 839, 1218, 1326, 1692 |
| HgaI | GACGC | 1 cut(s) | 2348 |
| HhaI | GCGC | 1 cut(s) | 947 |
| Hin1I | GRCGYC | 1 cut(s) | 2340 |
| Hin6I | GCGC | 1 cut(s) | 945 |
| HinP1I | GCGC | 1 cut(s) | 945 |
| HincII | GTYRAC | 1 cut(s) | 2131 |
| HindII | GTYRAC | 1 cut(s) | 2131 |
| HindIII | AAGCTT | 2 cut(s) | 628, 1557 |
| HinfI | GANTC | 8 cut(s) | 163, 648, 959, 1139, 1396, 1520, 1895, 2555 |
| HpaII | CCGG | 6 cut(s) | 434, 447, 839, 1218, 1326, 1692 |
| HphI | GGTGA | 5 cut(s) | 202, 1524, 1765, 2326, 2372 |
| Hpy166II | GTNNAC | 7 cut(s) | 330, 394, 971, 1133, 1969, 2131, 2500 |
| Hpy188III | TCNNGA | 3 cut(s) | 1285, 1553, 2336 |
| Hpy8I | GTNNAC | 7 cut(s) | 330, 394, 971, 1133, 1969, 2131, 2500 |
| HpyAV | CCTTC | 3 cut(s) | 448, 817, 1667 |
| HpyCH4III | ACNGT | 7 cut(s) | 91, 1424, 1578, 1973, 2084, 2140, 2444 |
| HpyCH4IV | ACGT | 6 cut(s) | 20, 250, 639, 981, 2034, 2408 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 360, 627, 1180, 1447, 1913, 2022, 2046, 2236 |
| HpySE526I | ACGT | 6 cut(s) | 20, 250, 639, 981, 2034, 2408 |
| Hsp92I | GRCGYC | 1 cut(s) | 2340 |
| HspAI | GCGC | 1 cut(s) | 945 |
| KroI | GCCGGC | 1 cut(s) | 1217 |
| KroNI | GCCGGC | 1 cut(s) | 1219 |
| Kzo9I | GATC | 8 cut(s) | 658, 855, 1047, 1371, 1401, 1481, 1584, 1992 |
| LguI | GCTCTTC | 2 cut(s) | 69, 849 |
| LmnI | GCTCC | 6 cut(s) | 346, 672, 912, 1001, 1677, 2030 |
| Lsp1109I | GCAGC | 6 cut(s) | 2025, 2412, 2415, 2448, 2518, 2533 |
| LweI | GCATC | 5 cut(s) | 204, 1065, 1509, 1642, 2170 |
| MaeI | CTAG | 8 cut(s) | 210, 389, 752, 1415, 1877, 1964, 2453, 2484 |
| MaeII | ACGT | 6 cut(s) | 20, 250, 639, 981, 2034, 2408 |
| MaeIII | GTNAC | 4 cut(s) | 865, 1060, 1424, 2416 |
| MalI | GATC | 8 cut(s) | 660, 857, 1049, 1373, 1403, 1483, 1586, 1994 |
| MboI | GATC | 8 cut(s) | 658, 855, 1047, 1371, 1401, 1481, 1584, 1992 |
| MflI | RGATCY | 4 cut(s) | 658, 1047, 1481, 1992 |
| MhlI | GDGCHC | 6 cut(s) | 610, 728, 909, 1026, 1390, 1980 |
| MlsI | TGGCCA | 2 cut(s) | 491, 1623 |
| MluCI | AATT | 7 cut(s) | 264, 306, 1031, 1092, 1157, 2199, 2284 |
| MluNI | TGGCCA | 2 cut(s) | 491, 1623 |
| MlyI | GAGTC | 4 cut(s) | 642, 1514, 1904, 2564 |
| MmeI | TCCRAC | 8 cut(s) | 41, 741, 1075, 1509, 1592, 1923, 2028, 2538 |
| Mox20I | TGGCCA | 2 cut(s) | 491, 1623 |
| Mph1103I | ATGCAT | 1 cut(s) | 1803 |
| MroNI | GCCGGC | 1 cut(s) | 1217 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1358 |
| MscI | TGGCCA | 2 cut(s) | 491, 1623 |
| MseI | TTAA | 6 cut(s) | 549, 663, 1091, 1238, 2156, 2493 |
| MslI | CAYNNNNRTG | 1 cut(s) | 218 |
| Msp20I | TGGCCA | 2 cut(s) | 491, 1623 |
| MspA1I | CMGCKG | 1 cut(s) | 35 |
| MspCI | CTTAAG | 2 cut(s) | 548, 662 |
| MspI | CCGG | 6 cut(s) | 434, 447, 839, 1218, 1326, 1692 |
| Mva1269I | GAATGC | 4 cut(s) | 213, 565, 1267, 2119 |
| MvaI | CCWGG | 8 cut(s) | 383, 407, 803, 1196, 1766, 2125, 2255, 2567 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 360, 627, 1180, 1447, 1913, 2022, 2046, 2236 |
| NaeI | GCCGGC | 1 cut(s) | 1219 |
| NciI | CCSGG | 2 cut(s) | 448, 839 |
| NcoI | CCATGG | 1 cut(s) | 1887 |
| NdeI | CATATG | 1 cut(s) | 1811 |
| NdeII | GATC | 8 cut(s) | 658, 855, 1047, 1371, 1401, 1481, 1584, 1992 |
| NgoMIV | GCCGGC | 1 cut(s) | 1217 |
| NlaIV | GGNNCC | 8 cut(s) | 288, 622, 734, 1003, 1334, 1483, 1885, 2270 |
| NmeAIII | GCCGAG | 3 cut(s) | 597, 1038, 1763 |
| NmuCI | GTSAC | 2 cut(s) | 1060, 2416 |
| NsiI | ATGCAT | 1 cut(s) | 1803 |
| NspI | RCATGY | 5 cut(s) | 328, 403, 1117, 1512, 2172 |
| PaeI | GCATGC | 2 cut(s) | 1512, 2172 |
| PagI | TCATGA | 1 cut(s) | 1284 |
| PaqCI | CACCTGC | 1 cut(s) | 2299 |
| PceI | AGGCCT | 1 cut(s) | 1299 |
| PciI | ACATGT | 3 cut(s) | 324, 399, 1113 |
| PciSI | GCTCTTC | 2 cut(s) | 69, 849 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2346 |
| PctI | GAATGC | 4 cut(s) | 213, 565, 1267, 2119 |
| PdiI | GCCGGC | 1 cut(s) | 1219 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1358 |
| PfeI | GAWTC | 4 cut(s) | 163, 959, 1139, 1396 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 504, 1116 |
| PkrI | GCNGC | 8 cut(s) | 776, 1234, 2015, 2402, 2405, 2438, 2523, 2533 |
| Ple19I | CGATCG | 1 cut(s) | 1404 |
| PleI | GAGTC | 4 cut(s) | 642, 1514, 1903, 2563 |
| PpsI | GAGTC | 4 cut(s) | 642, 1514, 1903, 2563 |
| Ppu21I | YACGTR | 1 cut(s) | 251 |
| PscI | ACATGT | 3 cut(s) | 324, 399, 1113 |
| PsiI | TTATAA | 2 cut(s) | 72, 102 |
| Psp124BI | GAGCTC | 2 cut(s) | 909, 1026 |
| Psp1406I | AACGTT | 1 cut(s) | 20 |
| Psp6I | CCWGG | 8 cut(s) | 381, 405, 801, 1194, 1764, 2123, 2253, 2565 |
| PspFI | CCCAGC | 3 cut(s) | 1363, 2114, 2322 |
| PspGI | CCWGG | 8 cut(s) | 381, 405, 801, 1194, 1764, 2123, 2253, 2565 |
| PspN4I | GGNNCC | 8 cut(s) | 288, 622, 734, 1003, 1334, 1483, 1885, 2270 |
| PspPI | GGNCC | 8 cut(s) | 287, 385, 772, 1333, 1348, 1406, 1969, 2269 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2442 |
| PsuI | RGATCY | 4 cut(s) | 658, 1047, 1481, 1992 |
| PvuI | CGATCG | 1 cut(s) | 1404 |
| PvuII | CAGCTG | 1 cut(s) | 35 |
| RsaI | GTAC | 5 cut(s) | 831, 970, 2252, 2375, 2458 |
| RsaNI | GTAC | 5 cut(s) | 830, 969, 2251, 2374, 2457 |
| RseI | CAYNNNNRTG | 1 cut(s) | 218 |
| SacI | GAGCTC | 2 cut(s) | 909, 1026 |
| SapI | GCTCTTC | 2 cut(s) | 69, 849 |
| SaqAI | TTAA | 6 cut(s) | 549, 663, 1091, 1238, 2156, 2493 |
| SatI | GCNGC | 8 cut(s) | 775, 1233, 2014, 2401, 2404, 2437, 2522, 2532 |
| Sau3AI | GATC | 8 cut(s) | 658, 855, 1047, 1371, 1401, 1481, 1584, 1992 |
| Sau96I | GGNCC | 8 cut(s) | 287, 385, 772, 1333, 1348, 1406, 1969, 2269 |
| SchI | GAGTC | 4 cut(s) | 642, 1514, 1904, 2564 |
| SduI | GDGCHC | 6 cut(s) | 610, 728, 909, 1026, 1390, 1980 |
| SfaNI | GCATC | 5 cut(s) | 204, 1065, 1509, 1642, 2170 |
| SfcI | CTRYAG | 3 cut(s) | 372, 1420, 2535 |
| SinI | GGWCC | 4 cut(s) | 1348, 1406, 1969, 2269 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 218 |
| SmlI | CTYRAG | 2 cut(s) | 548, 662 |
| SmoI | CTYRAG | 2 cut(s) | 548, 662 |
| SphI | GCATGC | 2 cut(s) | 1512, 2172 |
| Sse9I | AATT | 7 cut(s) | 264, 306, 1031, 1092, 1157, 2199, 2284 |
| SseBI | AGGCCT | 1 cut(s) | 1299 |
| SsiI | CCGC | 8 cut(s) | 775, 835, 859, 1233, 1536, 1632, 1823, 2047 |
| SspMI | CTAG | 8 cut(s) | 210, 389, 752, 1415, 1877, 1964, 2453, 2484 |
| SstI | GAGCTC | 2 cut(s) | 909, 1026 |
| StuI | AGGCCT | 1 cut(s) | 1299 |
| StyI | CCWWGG | 3 cut(s) | 1256, 1344, 1887 |
| TaaI | ACNGT | 7 cut(s) | 91, 1424, 1578, 1973, 2084, 2140, 2444 |
| TaiI | ACGT | 6 cut(s) | 23, 253, 642, 984, 2037, 2411 |
| TaqI | TCGA | 1 cut(s) | 646 |
| TasI | AATT | 7 cut(s) | 264, 306, 1031, 1092, 1157, 2199, 2284 |
| TatI | WGTACW | 2 cut(s) | 829, 2373 |
| TauI | GCSGC | 2 cut(s) | 777, 1235 |
| TfiI | GAWTC | 4 cut(s) | 163, 959, 1139, 1396 |
| Tru1I | TTAA | 6 cut(s) | 549, 663, 1091, 1238, 2156, 2493 |
| Tru9I | TTAA | 6 cut(s) | 549, 663, 1091, 1238, 2156, 2493 |
| TscAI | CASTG | 7 cut(s) | 96, 505, 1272, 1395, 1959, 2227, 2421 |
| TseFI | GTSAC | 2 cut(s) | 1060, 2416 |
| TseI | GCWGC | 6 cut(s) | 2013, 2400, 2403, 2436, 2521, 2531 |
| Tsp45I | GTSAC | 2 cut(s) | 1060, 2416 |
| TspDTI | ATGAA | 7 cut(s) | 730, 1168, 1265, 1301, 1686, 2053, 2140 |
| TspGWI | ACGGA | 4 cut(s) | 170, 431, 1748, 1896 |
| TspRI | CASTG | 7 cut(s) | 96, 505, 1272, 1395, 1959, 2227, 2421 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 504, 1116 |
| Vha464I | CTTAAG | 2 cut(s) | 548, 662 |
| VpaK11BI | GGWCC | 4 cut(s) | 1348, 1406, 1969, 2269 |
| XapI | RAATTY | 2 cut(s) | 264, 306 |
| XceI | RCATGY | 5 cut(s) | 328, 403, 1117, 1512, 2172 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 680, 1300, 2350 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1358 |
| XspI | CTAG | 8 cut(s) | 210, 389, 752, 1415, 1877, 1964, 2453, 2484 |
| Zsp2I | ATGCAT | 1 cut(s) | 1803 |