FvH4_7g31550

gamma-irradiation and mitomycin c induced

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001133
Physical Location & Seq
Forward (+)
22769734 .. 22784265
14532 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31550.t2

Sequence Viewer

Length: 4761 bp
ATGGATAAGAAATATAATTTCCAAATCCTATTACCAAATGGCACAACCGTCCGTTTGAAATTGCAGAATCCTGAGATGAAAATGCCATTCAGAGATTTCATTGAAAGGGTGGAAAAGGAATACATCCGCACTTGGAAACAATCAGGCTCGCTAAAGCGGAAGCGGGAGATTAACTGGAAAAGTGGTAGCTTTCTTCTTGTAGATGCTTATGATGTGAAGATACAAAACGTAGTGAATTTCAAGAATTTCAAGCCACAAGAATGTCACATTCTAAGACTCCAAGATGGTCTTGAGGACAGCACTACTACTTTTGAGAACATGTGGGATTTGACCCCCGACACTGATTTACTAAAGGAGCTACCCCAAGAGTATACATTTGAGACTGCTCTGGCAGATTTGATTGACAATTCTCTGCAAGCCGTATGGTCAAATGATAGGAGACATGGCAGACATATAAGTGTGGTTGCTGACGAAGATATGATCTCAATATTTGACAATGGCTCAGGGATGGATGCAAGTGATGAAAATTGTATAGTGAAGTGGGGAAAGATGGGTGCTTCACTTCACAGGTCTTATAAGGAACAGGCAATAGGGGGTAATCCTCCTTACCTAAAGCCTTTCTTTGGAATGTTTGGATATGGTGGACCTATAGCCTCCATGCAGTTGGGGAGGCGTGCTTTAGTTTCTTCTAAGACAAAGGATTCAAAGAAAGTTTATACCTTGAATCTTGATAGAAAGGCTTTACTCAGTGGGTCTTGGAAGACCGGTGGTAGCATGAGGGACCTTGAAGAAGATGAGATAAGCAGAGCACCACACGGAAGCTTTACTAAGGTTACAATCTTTGAACCTAAATCAAAAATGGACACATATCAACTCCAGTGCAAGCTGAAGGATATATATTTCCCTTATATTCAGTATGATGAAGATACAAAAAAAGGGAAGACCATAATGCCAGTCAAGTTTGAGGTCAATGGTGTTGATTTGGCTGAGGTTGAGGGTGGTGAGATTGCAATTACAAACATGCATTCCTGCAATGGTCCTGATTTTGTTTTACAGCTTCAGTTCTCCTTTAAGAAAGACAATATGAGTAAATATAAATCTCCAGATTCAAAGACTTATTGCGAAGCTAATGCTCGCCTGAAGTGCGTCTATTTTCCAATTGAACAGGGAAAAGAGAACATTGACAAAATTTTGGAGAAATTAGGGACAAGAGAAAACTTTGAGACCTTTAGTCGTGTCTCTATTCGGAGGCTTGGCCGCCTGCTTCCTGATGCTCGTTGGGGTTTGCTTCCGTTTATGGATCTGAAGCAAAAAAGAGGAACGACAGCACAATTGTTGAAAAAATGTTGCATGAGAGTTAAATGCTTTATAGAGACTGATGCTGGTTTCAATCCAACGTCATCGAAGACTGATCTTGCTCACCATAGTCCTTACACCACTGCATTGAGAAATCTCGGCAATAAGCCTCTTAAGAACGAGAATGACATGAATGTTCAACTGTATAAAGATGGAAACCTCTTAAGTCCTTCTCAACTGAAGAAGGAGTATGAGGACTGGATTATTGAGATGCATGCTCAATATGATCACGATGAAGCAGACTGTGGTGAAGACCAACCCGTCTTTCTTGTAAGTCCTGCAAACAAAAAGGCACTTCGGATATCATCTGAAGTTGCAAGGGTCCATAAATCATTAATGAGGCATGGAAGGACCTGGAAGTGTGGTCAAAGAATTAAAATTCTTAAGGGAGCATGTGTTGGCGTTCAGAGTAACAATGTTTATGCAACAATTGAATACTTTTTGCTTGAAGGTCTCCAAGATGAATCTGGTGGTGAGGCCCGTATTTTATGCAGGCCTTCAAGTCTGCCAGATGAAAAGGGGTGCATCCTTTCAGTCAATGATGGGAATACTAAATTGGAAATGGGTGAATCCTTGTCTGTACCACTAAGTGTGATTGATGCTGGAAAGTGCATAGCTGTTGGAAGAACTGAGTGGGAAGACCATATAGAGAGACGACGCCAGAAATCTTCTTCTTCAATTGAAGTTCTTGATGCAGAACAATGCCAAGAGTTGGAGCTTGATGGGGCATTACCTGTGGATGCTCGGGCTGGAAAGGTTCCTCAAGAAGAAATTGTGGCTGTTGTTCGACCTGGAAATTATGTGTATTCCAGTTCTTCAAAAAGCCTGGATCAGAAGTACGTCGTCAAAAGTAATTTAGAGATGTCTATGGAAGTTAGCTTCAGAGGCAATGCCAATGAACTTCAAAATGTTCGTCATATTTATTCAGTGCGCGTTGGACCTAAGTCACTTAAGGGAATTCAAGGGGTATATGTTTTTCCAGTTAAACACAAACTGTTGGGTTTCTTTCAAAGTGCTGGCATCTATACATTCTCATTTCATCTTAACGAGTCAGATTGTAAGAGTGCTGAGAAAAGAGTACGAGTCAAGCCATCTTCCAAGGTTGGAAAGTGGGTCCTTCTAAACGATGATCAGCCTCCACTGTACCAAGTGAGAGTTGGTTCAGTGTTTCCACCTCTCTCTATTGCATGTTACGATGTATATGACAATCAAATTCCATTTGCAACTACTCTGGAAGTCGCAGTGAAAGTTCAGACAGATGAGGGTTTGCTTTTCCATGTAGAAAAATTCACAAAAGAATTCTCAAACCATACGTTGACAGTAAAGGATATGATGATGGAGAGCAGTGAGTTAGACAAACTTCGACCTACATATGAAGCTACTTTAGTTGTAAGTTCAGAAGATGAAAACATTTCAGTTTTGGTTCCGTGCAAAGTTGATCCTGGACCTCTACAGACTGTCAAGGCTATTCCTCCTATTTTCGAAAACCAAGTGCTTCCAGGTTACACTATTCAGGAGTTTATTTTAGAGATGTTTGATCAATATGGCAATCATGTAATTGAAGGCACTGAAGTTCAATTGAATGTGGAGGGGTTTTCTATTCAAGATCGACTGGGTACAACACGTAAGGCTGATCATCATGGATGCATTGACCTTGGTGGCCTGTTGAAAGTAACCGCAGGTTATGAGAAAAATGTATCATTTTCTGTCTACTACAATAATGATGTTTTACTGATGCTTCAGTCTCAAACTGAGAAAAGGGTGTTGAAAATTTCATCTAAGGTGCCTGAGGTTTGTGTGGTCGGTACTCAAATGGAAAATTTGGTCTTTGAAATCATCAACTCTGAAGGTGTTGTTGATGATACTTTTCATCATGAAGAGAAAAGTGGCCAATTACATATGCTTACAATAAAGGCCGGTTCATTTATGGAGGAATCCCTTCGTTTCACATTCAAGCATGGCCGTTGTACTGTTACGGGCCTTTCTGTTCCTGAAGTGGAAGAGAGTTTTAACTTTGTAGCTGCTCATTCATATTATCCAGAACTGCATGTGAATGTAGAGGTTCCTGTAGTGCGACCTGTAAAAGAGAAATATGATCATCTTCTCATTAAAAAAGAGAAGCAAGTTGAGTCTCCAACTCTGTGGGATGTTTCTCCGCTCCATGAGTTGCCGCAGCAAGTAGGAAATTTAAGGGTTCCAAAAGTGGAACATCAAGAGTTTCAGTCTCCTAGTTCAATTGGAAACACATTTCCCTCTCCAGAGTCTTCTTGCCTTCTGCAATTAGAAAATGTTAAGGAACTGAAGAATATCATGCACCAACATGGACTATGTGTTGGAGATGTGGAAGAAAAACTGAAGATTCTAGAAAAAGAAAAAGAAAAAGCTAAGCAAGAGCTCTCTGATTTACAAGGGTCTATTGAACCTCACAAAGTGGAATCGATAAACCGCATTGAAAGCATGAATCACAGTGCAGCTGCTATCATCTGTACTCTATTTCGAGAAGTTTCATTCGAAGAGGCAAACAATCAATTCATGGAAGATGTGATTGGATTGGTTGCTCTACTTGGCACAGTTGGTAGTAGTAATCTTAGCAGGATATTGGCTGAATACTTGGGGAAAGATCAGATGCTTGCTATTGTCTGTAGATCCTTTGAAGCTGCTGGTGCTCTTGAGAAGTTTGTACAGAAACCTAAGAAACCTATGTATGGGGAAGTGGATTCTATGCATGCTCTCAGTGAACCAGCTCTACTTCAGGAACCTACACTTGGTCGATCTTTTGTCATTTGTTTGGAAGACATAAGGCCTTACTCTGGTGACTTTGACCGTAGTGACCCTCAAAGGAAACTGGCATTGCCAGATCCAGTCCTGCCAAGTGGAATGACTCCTGATGGTTTCTTAGGATATGCAGTCAATATGGTTGACTTGGATAGCCATCATATACACACAGTGACATCTGCAGGCCACGGTCTCAGGGAAACAGTTTTATATTGTCTTCTTGGCGAGCTCCAAGTATATAAAACCAGGGAGGATATGTTGGCTGCTCGTGCTTGTATTAAACATGGTGCTATTTCGCTGGATGGAGGAATTCTGAAACAAAATGGAGTAACTTCTTTTGGAGTCGGGAACGTTGAAATACGTTTTCCAGTAGCAGAAAATCTGGAGCAGCCGACATCAACAAATGTTGTTGAAGTTGAGAAGCAGATTGAGGAGAAGAAGTCAAAGTTGAAAATGATCAATAAAGCTATAGAATTGGCAAACAAATTGCATGGGAAATCAGTGAAGAGATTCAAGAGGACAAAGAAGTCGATGCATAAGCTATTGGAGAACATCCGCCGCTCAAGCCAAGAGCAGTCTCCCAGTCCAATGAAAGAAGGGAGACAACTCGTAATGGCTATCAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c_3

Protein Analysis

1587

Amino Acids

178.74

Weight (kDa)

6.29

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 576
AasI GACNNNNNNGTC 2 cut(s) 1616, 4657
Acc36I ACCTGC 1 cut(s) 3026
AccB1I GGYRCC 1 cut(s) 3139
AccB7I CCANNNNNTGG 1 cut(s) 2068
AccBSI CCGCTC 2 cut(s) 3514, 4692
AccI GTMKAC 2 cut(s) 371, 3066
AccII CGCG 1 cut(s) 2289
AclI AACGTT 1 cut(s) 4482
AclWI GGATC 5 cut(s) 1308, 2193, 2789, 3996, 4208
AcoI YGGCCR 3 cut(s) 1255, 3244, 3316
AcyI GRCGYC 1 cut(s) 2014
AdeI CACNNNGTG 3 cut(s) 1946, 3787, 4303
AfaI GTAC 9 cut(s) 1938, 2195, 2436, 2501, 2974, 3163, 3325, 3844, 4038
AfiI CCNNNNNNNGG 5 cut(s) 623, 2068, 4061, 4121, 4166
AflII CTTAAG 4 cut(s) 1469, 1519, 1739, 2306
AflIII ACRYGT 2 cut(s) 318, 2978
AgeI ACCGGT 1 cut(s) 764
AhdI GACNNNNNGTC 1 cut(s) 1230
AjnI CCWGG 6 cut(s) 1709, 2146, 2181, 2797, 2854, 4376
AjuI GAANNNNNNNTTGG 8 cut(s) 1605, 1637, 1895, 1927, 3885, 3917, 4104, 4136
Alw21I GWGCWC 4 cut(s) 811, 3753, 4024, 4362
AlwI GGATC 5 cut(s) 1308, 2193, 2789, 3996, 4208
Ama87I CYCGRG 1 cut(s) 2100
ApeKI GCWGC 7 cut(s) 3377, 3529, 3827, 3830, 4013, 4394, 4519
ArsI GACNNNNNNTTYG 2 cut(s) 2254, 2286
AseI ATTAAT 1 cut(s) 1691
AsiGI ACCGGT 1 cut(s) 764
Asp700I GAANNNNTTC 2 cut(s) 3294, 4640
AspLEI GCGC 1 cut(s) 2289
AsuHPI GGTGA 6 cut(s) 1013, 1412, 1616, 1841, 1934, 4181
AsuII TTCGAA 2 cut(s) 2838, 3867
AvaI CYCGRG 1 cut(s) 2100
AvaII GGWCC 8 cut(s) 644, 781, 1037, 1678, 1707, 2294, 2470, 2801
AxyI CCTNAGG 1 cut(s) 3144
BalI TGGCCA 1 cut(s) 3246
BanI GGYRCC 1 cut(s) 3139
BanII GRGCYC 2 cut(s) 3753, 4362
BauI CACGAG 1 cut(s) 4398
BbsI GAAGAC 8 cut(s) 767, 947, 1412, 1614, 2001, 3612, 4155, 4340
Bbv12I GWGCWC 4 cut(s) 811, 3753, 4024, 4362
BbvCI CCTCAGC 1 cut(s) 987
BbvI GCAGC 7 cut(s) 3364, 3541, 3817, 3839, 4000, 4381, 4531
BceAI ACGGC 2 cut(s) 404, 3303
BcgI CGANNNNNNTGC 2 cut(s) 1112, 1146
BciT130I CCWGG 6 cut(s) 1711, 2148, 2183, 2799, 2856, 4378
BclI TGATCA 6 cut(s) 1582, 2485, 2893, 2989, 3451, 4587
BfaI CTAG 2 cut(s) 3585, 3719
BfmI CTRYAG 6 cut(s) 648, 2807, 3423, 3997, 4311, 4599
BfrI CTTAAG 4 cut(s) 1469, 1519, 1739, 2306
BfuAI ACCTGC 1 cut(s) 3026
BlpI GCTNAGC 1 cut(s) 3741
Bme1390I CCNGG 6 cut(s) 1711, 2148, 2183, 2799, 2856, 4378
Bme18I GGWCC 8 cut(s) 644, 781, 1037, 1678, 1707, 2294, 2470, 2801
BmeRI GACNNNNNGTC 1 cut(s) 1230
BmeT110I CYCGRG 1 cut(s) 2100
BmiI GGNNCC 9 cut(s) 782, 1679, 2115, 2471, 2781, 3141, 3420, 3552, 4113
BmrFI CCNGG 6 cut(s) 1711, 2148, 2183, 2799, 2856, 4378
BmrI ACTGGG 2 cut(s) 2978, 4707
BmuI ACTGGG 2 cut(s) 2978, 4707
BoxI GACNNNNGTC 1 cut(s) 2299
BpiI GAAGAC 8 cut(s) 767, 947, 1412, 1614, 2001, 3612, 4155, 4340
BpmI CTGGAG 4 cut(s) 860, 1086, 3597, 4535
Bpu10I CCTNAGC 2 cut(s) 502, 987
Bpu1102I GCTNAGC 1 cut(s) 3741
Bpu14I TTCGAA 2 cut(s) 2838, 3867
BpuEI CTTGAG 4 cut(s) 311, 2103, 4046, 4678
Bsa29I ATCGAT 1 cut(s) 3794
BsaAI YACGTR 1 cut(s) 2981
BsaBI GATNNNNATC 1 cut(s) 4287
BsaHI GRCGYC 1 cut(s) 2014
BsaI GGTCTC 3 cut(s) 1217, 1814, 4328
BsaJI CCNNGG 4 cut(s) 2454, 3010, 4318, 4377
BsaWI WCCGGW 1 cut(s) 764
BsaXI ACNNNNNCTCC 6 cut(s) 1737, 1767, 4373, 4403, 4556, 4586
Bsc4I CCNNNNNNNGG 5 cut(s) 623, 2068, 4061, 4121, 4166
Bse118I RCCGGY 2 cut(s) 764, 3272
Bse21I CCTNAGG 1 cut(s) 3144
Bse3DI GCAATG 3 cut(s) 1039, 2251, 4205
Bse8I GATNNNNATC 1 cut(s) 4287
BseBI CCWGG 6 cut(s) 1711, 2148, 2183, 2799, 2856, 4378
BseCI ATCGAT 1 cut(s) 3794
BseDI CCNNGG 4 cut(s) 2454, 3010, 4318, 4377
BseGI GGATG 9 cut(s) 123, 513, 517, 1881, 2101, 3005, 3508, 4438, 4683
BseJI GATNNNNATC 1 cut(s) 4287
BseLI CCNNNNNNNGG 5 cut(s) 623, 2068, 4061, 4121, 4166
BseMI GCAATG 3 cut(s) 1039, 2251, 4205
BseRI GAGGAG 1 cut(s) 4577
BseXI GCAGC 7 cut(s) 3364, 3541, 3817, 3839, 4000, 4381, 4531
BsgI GTGCAG 1 cut(s) 3846
Bsh1236I CGCG 1 cut(s) 2289
BshNI GGYRCC 1 cut(s) 3139
BshTI ACCGGT 1 cut(s) 764
BshVI ATCGAT 1 cut(s) 3794
BsiHKAI GWGCWC 4 cut(s) 811, 3753, 4024, 4362
BsiHKCI CYCGRG 1 cut(s) 2100
BsiSI CCGG 2 cut(s) 765, 3273
BslFI GGGAC 2 cut(s) 794, 1219
BslI CCNNNNNNNGG 5 cut(s) 623, 2068, 4061, 4121, 4166
BsmBI CGTCTC 1 cut(s) 2002
BsmFI GGGAC 2 cut(s) 794, 1219
BsmI GAATGC 1 cut(s) 1024
Bso31I GGTCTC 3 cut(s) 1217, 1814, 4328
BsoBI CYCGRG 1 cut(s) 2100
Bsp119I TTCGAA 2 cut(s) 2838, 3867
Bsp1286I GDGCHC 4 cut(s) 811, 3753, 4024, 4362
Bsp1407I TGTACA 1 cut(s) 4036
Bsp1720I GCTNAGC 1 cut(s) 3741
BspDI ATCGAT 1 cut(s) 3794
BspFNI CGCG 1 cut(s) 2289
BspHI TCATGA 1 cut(s) 3229
BspLI GGNNCC 9 cut(s) 782, 1679, 2115, 2471, 2781, 3141, 3420, 3552, 4113
BspMAI CTGCAG 1 cut(s) 4315
BspMI ACCTGC 1 cut(s) 3026
BspPI GGATC 5 cut(s) 1308, 2193, 2789, 3996, 4208
BspT104I TTCGAA 2 cut(s) 2838, 3867
BspT107I GGYRCC 1 cut(s) 3139
BspTI CTTAAG 4 cut(s) 1469, 1519, 1739, 2306
BspTNI GGTCTC 3 cut(s) 1217, 1814, 4328
BsrBI CCGCTC 2 cut(s) 3514, 4692
BsrDI GCAATG 3 cut(s) 1039, 2251, 4205
BsrFI RCCGGY 2 cut(s) 764, 3272
BsrGI TGTACA 1 cut(s) 4036
BssAI RCCGGY 2 cut(s) 764, 3272
BssECI CCNNGG 4 cut(s) 2454, 3010, 4318, 4377
BssNAI GTATAC 1 cut(s) 372
BssNI GRCGYC 1 cut(s) 2014
BssSI CACGAG 1 cut(s) 4398
BssT1I CCWWGG 2 cut(s) 2454, 3010
Bst1107I GTATAC 1 cut(s) 372
Bst2BI CACGAG 1 cut(s) 4398
Bst2UI CCWGG 6 cut(s) 1711, 2148, 2183, 2799, 2856, 4378
Bst6I CTCTTC 4 cut(s) 3228, 3351, 3864, 4631
BstACI GRCGYC 1 cut(s) 2014
BstAFI CTTAAG 4 cut(s) 1469, 1519, 1739, 2306
BstAUI TGTACA 1 cut(s) 4036
BstBAI YACGTR 1 cut(s) 2981
BstBI TTCGAA 2 cut(s) 2838, 3867
BstDSI CCRYGG 1 cut(s) 4318
BstF5I GGATG 9 cut(s) 123, 513, 517, 1881, 2101, 3005, 3508, 4438, 4683
BstFNI CGCG 1 cut(s) 2289
BstHHI GCGC 1 cut(s) 2289
BstMWI GCNNNNNNNGC 6 cut(s) 1143, 2241, 3810, 4019, 4400, 4695
BstNI CCWGG 6 cut(s) 1711, 2148, 2183, 2799, 2856, 4378
BstNSI RCATGY 7 cut(s) 322, 1024, 1574, 1752, 2547, 3407, 4085
BstPAI GACNNNNGTC 1 cut(s) 2299
BstSCI CCNGG 6 cut(s) 1709, 2146, 2181, 2797, 2854, 4376
BstSFI CTRYAG 6 cut(s) 648, 2807, 3423, 3997, 4311, 4599
BstUI CGCG 1 cut(s) 2289
BstV1I GCAGC 7 cut(s) 3364, 3541, 3817, 3839, 4000, 4381, 4531
BstV2I GAAGAC 8 cut(s) 767, 947, 1412, 1614, 2001, 3612, 4155, 4340
BstX2I RGATCY 3 cut(s) 1300, 4001, 4213
BstXI CCANNNNNNTGG 2 cut(s) 664, 3498
BstYI RGATCY 3 cut(s) 1300, 4001, 4213
BstZ17I GTATAC 1 cut(s) 372
Bsu15I ATCGAT 1 cut(s) 3794
Bsu36I CCTNAGG 1 cut(s) 3144
BsuTUI ATCGAT 1 cut(s) 3794
BtgI CCRYGG 1 cut(s) 4318
BtsCI GGATG 9 cut(s) 123, 513, 517, 1881, 2101, 3005, 3508, 4438, 4683
BtsI GCAGTG 3 cut(s) 1437, 2604, 2707
BveI ACCTGC 1 cut(s) 3026
CciI TCATGA 1 cut(s) 3229
CfoI GCGC 1 cut(s) 2289
Cfr10I RCCGGY 2 cut(s) 764, 3272
ClaI ATCGAT 1 cut(s) 3794
CseI GACGC 2 cut(s) 1135, 2022
Csp6I GTAC 9 cut(s) 1937, 2194, 2435, 2500, 2973, 3162, 3324, 3843, 4037
CspAI ACCGGT 1 cut(s) 764
CviQI GTAC 9 cut(s) 1937, 2194, 2435, 2500, 2973, 3162, 3324, 3843, 4037
DraIII CACNNNGTG 3 cut(s) 1946, 3787, 4303
DrdI GACNNNNNNGTC 2 cut(s) 1616, 4657
DriI GACNNNNNGTC 1 cut(s) 1230
DseDI GACNNNNNNGTC 2 cut(s) 1616, 4657
EaeI YGGCCR 3 cut(s) 1255, 3244, 3316
Eam1104I CTCTTC 4 cut(s) 3228, 3351, 3864, 4631
Eam1105I GACNNNNNGTC 1 cut(s) 1230
EarI CTCTTC 4 cut(s) 3228, 3351, 3864, 4631
EciI GGCGGA 1 cut(s) 4676
Ecl136II GAGCTC 2 cut(s) 3751, 4360
Eco130I CCWWGG 2 cut(s) 2454, 3010
Eco147I AGGCCT 2 cut(s) 1852, 4159
Eco24I GRGCYC 2 cut(s) 3753, 4362
Eco31I GGTCTC 3 cut(s) 1217, 1814, 4328
Eco32I GATATC 1 cut(s) 1659
Eco47I GGWCC 8 cut(s) 644, 781, 1037, 1678, 1707, 2294, 2470, 2801
Eco53kI GAGCTC 2 cut(s) 3751, 4360
Eco81I CCTNAGG 1 cut(s) 3144
Eco88I CYCGRG 1 cut(s) 2100
EcoICRI GAGCTC 2 cut(s) 3751, 4360
EcoO109I RGGNCCY 3 cut(s) 781, 1707, 2470
EcoRI GAATTC 3 cut(s) 2313, 2654, 4440
EcoRII CCWGG 6 cut(s) 1709, 2146, 2181, 2797, 2854, 4376
EcoRV GATATC 1 cut(s) 1659
EcoT14I CCWWGG 2 cut(s) 2454, 3010
EcoT22I ATGCAT 5 cut(s) 1026, 1572, 3005, 4083, 4668
EcoT38I GRGCYC 2 cut(s) 3753, 4362
ErhI CCWWGG 2 cut(s) 2454, 3010
Esp3I CGTCTC 1 cut(s) 2002
FalI AAGNNNNNCTT 4 cut(s) 273, 305, 605, 637
FaqI GGGAC 2 cut(s) 794, 1219
FauI CCCGC 1 cut(s) 156
FauNDI CATATG 2 cut(s) 2728, 3255
FbaI TGATCA 6 cut(s) 1582, 2485, 2893, 2989, 3451, 4587
FblI GTMKAC 2 cut(s) 371, 3066
FokI GGATG 9 cut(s) 110, 520, 524, 1868, 2108, 3012, 3515, 4445, 4670
FriOI GRGCYC 2 cut(s) 3753, 4362
FspBI CTAG 2 cut(s) 3585, 3719
GlaI GCGC 1 cut(s) 2288
GsuI CTGGAG 4 cut(s) 860, 1086, 3597, 4535
HapII CCGG 2 cut(s) 765, 3273
HgaI GACGC 2 cut(s) 1135, 2022
HhaI GCGC 1 cut(s) 2289
Hin1I GRCGYC 1 cut(s) 2014
Hin6I GCGC 1 cut(s) 2287
HinP1I GCGC 1 cut(s) 2287
HincII GTYRAC 2 cut(s) 2673, 4276
HindII GTYRAC 2 cut(s) 2673, 4276
HindIII AAGCTT 1 cut(s) 820
HpaII CCGG 2 cut(s) 765, 3273
HphI GGTGA 6 cut(s) 1013, 1412, 1616, 1841, 1934, 4181
Hpy166II GTNNAC 6 cut(s) 372, 644, 2673, 3067, 4094, 4276
Hpy8I GTNNAC 6 cut(s) 372, 644, 2673, 3067, 4094, 4276
Hpy99I CGWCG 2 cut(s) 2016, 2201
HpyCH4IV ACGT 7 cut(s) 228, 1397, 2196, 2669, 2980, 4482, 4492
HpyF10VI GCNNNNNNNGC 6 cut(s) 1143, 2241, 3810, 4019, 4400, 4695
HpySE526I ACGT 7 cut(s) 228, 1397, 2196, 2669, 2980, 4482, 4492
Hsp92I GRCGYC 1 cut(s) 2014
HspAI GCGC 1 cut(s) 2287
Ksp22I TGATCA 6 cut(s) 1582, 2485, 2893, 2989, 3451, 4587
LmnI GCTCC 6 cut(s) 355, 1745, 2071, 3519, 4365, 4516
Lsp1109I GCAGC 7 cut(s) 3364, 3541, 3817, 3839, 4000, 4381, 4531
MaeI CTAG 2 cut(s) 3585, 3719
MaeII ACGT 7 cut(s) 228, 1397, 2196, 2669, 2980, 4482, 4492
MbiI CCGCTC 2 cut(s) 3514, 4692
MfeI CAATTG 6 cut(s) 1158, 1331, 1785, 2034, 2933, 3591
MflI RGATCY 3 cut(s) 1300, 4001, 4213
MhlI GDGCHC 4 cut(s) 811, 3753, 4024, 4362
MlsI TGGCCA 1 cut(s) 3246
MluNI TGGCCA 1 cut(s) 3246
MlyI GAGTC 7 cut(s) 270, 2414, 2448, 3494, 3626, 4231, 4482
MmeI TCCRAC 7 cut(s) 1418, 1957, 2049, 2272, 2440, 3515, 3670
Mox20I TGGCCA 1 cut(s) 3246
Mph1103I ATGCAT 5 cut(s) 1026, 1572, 3005, 4083, 4668
MroXI GAANNNNTTC 2 cut(s) 3294, 4640
MscI TGGCCA 1 cut(s) 3246
MslI CAYNNNNRTG 4 cut(s) 259, 456, 3408, 3675
Msp20I TGGCCA 1 cut(s) 3246
MspA1I CMGCKG 1 cut(s) 3830
MspCI CTTAAG 4 cut(s) 1469, 1519, 1739, 2306
MspI CCGG 2 cut(s) 765, 3273
MspR9I CCNGG 6 cut(s) 1711, 2148, 2183, 2799, 2856, 4378
MunI CAATTG 6 cut(s) 1158, 1331, 1785, 2034, 2933, 3591
Mva1269I GAATGC 1 cut(s) 1024
MvaI CCWGG 6 cut(s) 1711, 2148, 2183, 2799, 2856, 4378
MvnI CGCG 1 cut(s) 2289
MwoI GCNNNNNNNGC 6 cut(s) 1143, 2241, 3810, 4019, 4400, 4695
NdeI CATATG 2 cut(s) 2728, 3255
NlaIV GGNNCC 9 cut(s) 782, 1679, 2115, 2471, 2781, 3141, 3420, 3552, 4113
NmeAIII GCCGAG 1 cut(s) 1434
NmuCI GTSAC 5 cut(s) 263, 2301, 4169, 4184, 4303
NsiI ATGCAT 5 cut(s) 1026, 1572, 3005, 4083, 4668
NspI RCATGY 7 cut(s) 322, 1024, 1574, 1752, 2547, 3407, 4085
NspV TTCGAA 2 cut(s) 2838, 3867
PaeI GCATGC 2 cut(s) 1574, 4085
PagI TCATGA 1 cut(s) 3229
PceI AGGCCT 2 cut(s) 1852, 4159
PciI ACATGT 1 cut(s) 318
PctI GAATGC 1 cut(s) 1024
PdmI GAANNNNTTC 2 cut(s) 3294, 4640
PflMI CCANNNNNTGG 1 cut(s) 2068
PfoI TCCNGGA 1 cut(s) 2797
PinAI ACCGGT 1 cut(s) 764
PleI GAGTC 7 cut(s) 270, 2413, 2447, 3493, 3625, 4231, 4481
PpsI GAGTC 7 cut(s) 270, 2413, 2447, 3493, 3625, 4231, 4481
Ppu21I YACGTR 1 cut(s) 2981
PpuMI RGGWCCY 3 cut(s) 781, 1707, 2470
PscI ACATGT 1 cut(s) 318
PshAI GACNNNNGTC 1 cut(s) 2299
PshBI ATTAAT 1 cut(s) 1691
PsiI TTATAA 1 cut(s) 576
Psp124BI GAGCTC 2 cut(s) 3753, 4362
Psp1406I AACGTT 1 cut(s) 4482
Psp5II RGGWCCY 3 cut(s) 781, 1707, 2470
Psp6I CCWGG 6 cut(s) 1709, 2146, 2181, 2797, 2854, 4376
PspGI CCWGG 6 cut(s) 1709, 2146, 2181, 2797, 2854, 4376
PspN4I GGNNCC 9 cut(s) 782, 1679, 2115, 2471, 2781, 3141, 3420, 3552, 4113
PspPPI RGGWCCY 3 cut(s) 781, 1707, 2470
PsrI GAACNNNNNNTAC 2 cut(s) 4086, 4118
PstI CTGCAG 1 cut(s) 4315
PsuI RGATCY 3 cut(s) 1300, 4001, 4213
PvuII CAGCTG 1 cut(s) 3830
RsaI GTAC 9 cut(s) 1938, 2195, 2436, 2501, 2974, 3163, 3325, 3844, 4038
RsaNI GTAC 9 cut(s) 1937, 2194, 2435, 2500, 2973, 3162, 3324, 3843, 4037
RseI CAYNNNNRTG 4 cut(s) 259, 456, 3408, 3675
SacI GAGCTC 2 cut(s) 3753, 4362
SchI GAGTC 7 cut(s) 270, 2414, 2448, 3494, 3626, 4231, 4482
ScrFI CCNGG 6 cut(s) 1711, 2148, 2183, 2799, 2856, 4378
SduI GDGCHC 4 cut(s) 811, 3753, 4024, 4362
SfcI CTRYAG 6 cut(s) 648, 2807, 3423, 3997, 4311, 4599
SfuI TTCGAA 2 cut(s) 2838, 3867
SinI GGWCC 8 cut(s) 644, 781, 1037, 1678, 1707, 2294, 2470, 2801
SmiMI CAYNNNNRTG 4 cut(s) 259, 456, 3408, 3675
SmlI CTYRAG 8 cut(s) 290, 1469, 1519, 1739, 2118, 2306, 4025, 4693
SmoI CTYRAG 8 cut(s) 290, 1469, 1519, 1739, 2118, 2306, 4025, 4693
SphI GCATGC 2 cut(s) 1574, 4085
SseBI AGGCCT 2 cut(s) 1852, 4159
SspI AATATT 1 cut(s) 489
SspMI CTAG 2 cut(s) 3585, 3719
SstI GAGCTC 2 cut(s) 3753, 4362
StuI AGGCCT 2 cut(s) 1852, 4159
StyD4I CCNGG 6 cut(s) 1709, 2146, 2181, 2797, 2854, 4376
StyI CCWWGG 2 cut(s) 2454, 3010
TaiI ACGT 7 cut(s) 231, 1400, 2199, 2672, 2983, 4485, 4495
TatI WGTACW 3 cut(s) 3323, 3842, 4036
TauI GCSGC 3 cut(s) 1260, 3529, 4692
TseFI GTSAC 5 cut(s) 263, 2301, 4169, 4184, 4303
TseI GCWGC 7 cut(s) 3377, 3529, 3827, 3830, 4013, 4394, 4519
Tsp45I GTSAC 5 cut(s) 263, 2301, 4169, 4184, 4303
TspGWI ACGGA 4 cut(s) 41, 831, 1281, 2772
Van91I CCANNNNNTGG 1 cut(s) 2068
Vha464I CTTAAG 4 cut(s) 1469, 1519, 1739, 2306
VpaK11BI GGWCC 8 cut(s) 644, 781, 1037, 1678, 1707, 2294, 2470, 2801
VspI ATTAAT 1 cut(s) 1691
XbaI TCTAGA 1 cut(s) 3718
XceI RCATGY 7 cut(s) 322, 1024, 1574, 1752, 2547, 3407, 4085
XcmI CCANNNNNNNNNTGG 2 cut(s) 1820, 2510
XmiI GTMKAC 2 cut(s) 371, 3066
XmnI GAANNNNTTC 2 cut(s) 3294, 4640
XspI CTAG 2 cut(s) 3585, 3719
Zsp2I ATGCAT 5 cut(s) 1026, 1572, 3005, 4083, 4668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.