Rorug01G0422200

gamma-irradiation and mitomycin c induced

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001133
Physical Location & Seq
Reverse (-)
52324027 .. 52326126
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0422200.1

Sequence Viewer

Length: 357 bp
ATGCATATATTGGTTTTTACGTTTGACTCTAACTCTCAAGATTTCCTTTTCATTGCACAGGTTGGTGGGAAGGTGAATGAGGTACCTGTAATAAGAATTTACGGCTCTACTCCAGCCGGTCAGAAAGCTTGTCTGCACATTCACAGACCATTACCATACCTATTTGTGCCGTGCAAAGATATACCGATTCAACCACATCAGAAAGGTGATTCGTACACATATGATATATCTCTTGCATTAGAGAAAGCTTTGAAGGCAAAAGCAGTGCTGGTTCAAAACGACAACATGTACATGGTTGCAGCCTTGTCCAGGCAAGGAAGCTTTACGGTTACCATTCATCAGAGGAGGTGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c_3

Protein Analysis

118

Amino Acids

13.25

Weight (kDa)

9.1

Isoelectric Point (pI)

61.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REV3_N PF24065 23 - 48 5.1e-12 DNA polymerase zeta catalytic subunit, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 82
AccB1I GGYRCC 1 cut(s) 82
AcsI RAATTY 1 cut(s) 96
AfaI GTAC 3 cut(s) 84, 215, 290
AfiI CCNNNNNNNGG 1 cut(s) 309
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 3 cut(s) 191, 253, 275
AjnI CCWGG 1 cut(s) 308
AluBI AGCT 3 cut(s) 128, 248, 321
AluI AGCT 3 cut(s) 128, 248, 321
ApeKI GCWGC 1 cut(s) 299
ApoI RAATTY 1 cut(s) 96
Asp718I GGTACC 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 85, 218
BanI GGYRCC 1 cut(s) 82
BbvI GCAGC 1 cut(s) 311
BceAI ACGGC 2 cut(s) 118, 154
BciT130I CCWGG 1 cut(s) 310
BisI GCNGC 1 cut(s) 300
BlsI GCNGC 1 cut(s) 301
Bme1390I CCNGG 1 cut(s) 310
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 21
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse118I RCCGGY 1 cut(s) 116
Bse3DI GCAATG 1 cut(s) 51
BseBI CCWGG 1 cut(s) 310
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMI GCAATG 1 cut(s) 51
BseXI GCAGC 1 cut(s) 311
BsgI GTGCAG 1 cut(s) 119
BshNI GGYRCC 1 cut(s) 82
BsiSI CCGG 1 cut(s) 117
BslI CCNNNNNNNGG 1 cut(s) 309
Bsp1407I TGTACA 1 cut(s) 288
BspLI GGNNCC 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 51
BsrFI RCCGGY 1 cut(s) 116
BsrGI TGTACA 1 cut(s) 288
BssAI RCCGGY 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 310
Bst4CI ACNGT 1 cut(s) 328
BstAUI TGTACA 1 cut(s) 288
BstEII GGTNACC 1 cut(s) 328
BstENI CCTNNNNNAGG 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 1 cut(s) 310
BstNSI RCATGY 1 cut(s) 289
BstPI GGTNACC 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 308
BstV1I GCAGC 1 cut(s) 311
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 1 cut(s) 270
Cfr10I RCCGGY 1 cut(s) 116
Csp6I GTAC 3 cut(s) 83, 214, 289
CviAII CATG 2 cut(s) 286, 292
CviJI RGCY 6 cut(s) 105, 116, 128, 248, 302, 321
CviKI_1 RGCY 6 cut(s) 105, 116, 128, 248, 302, 321
CviQI GTAC 3 cut(s) 83, 214, 289
Eco91I GGTNACC 1 cut(s) 328
EcoNI CCTNNNNNAGG 1 cut(s) 307
EcoO65I GGTNACC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 308
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 289, 295
FaiI YATR 9 cut(s) 6, 8, 157, 182, 220, 222, 227, 287, 293
FalI AAGNNNNNCTT 2 cut(s) 30, 62
FatI CATG 2 cut(s) 285, 291
FauNDI CATATG 1 cut(s) 220
Fnu4HI GCNGC 1 cut(s) 300
Fsp4HI GCNGC 1 cut(s) 300
GluI GCNGC 1 cut(s) 300
GsuI CTGGAG 1 cut(s) 96
HapII CCGG 1 cut(s) 117
Hin1II CATG 2 cut(s) 289, 295
HindIII AAGCTT 3 cut(s) 126, 246, 319
HinfI GANTC 3 cut(s) 26, 187, 209
HpaII CCGG 1 cut(s) 117
HphI GGTGA 2 cut(s) 85, 218
Hpy166II GTNNAC 1 cut(s) 216
Hpy188I TCNGA 3 cut(s) 123, 201, 342
Hpy188III TCNNGA 1 cut(s) 38
Hpy8I GTNNAC 1 cut(s) 216
HpyAV CCTTC 2 cut(s) 64, 247
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 6 cut(s) 4, 56, 136, 174, 236, 299
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpySE526I ACGT 1 cut(s) 20
Hsp92II CATG 2 cut(s) 289, 295
KpnI GGTACC 1 cut(s) 86
LpnPI CCDG 7 cut(s) 44, 99, 126, 130, 254, 295, 322
Lsp1109I GCAGC 1 cut(s) 311
MaeII ACGT 1 cut(s) 20
MaeIII GTNAC 1 cut(s) 328
MluCI AATT 1 cut(s) 96
MlyI GAGTC 1 cut(s) 20
MnlI CCTC 3 cut(s) 73, 336, 339
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 290
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 310
MvaI CCWGG 1 cut(s) 310
MwoI GCNNNNNNNGC 1 cut(s) 254
NdeI CATATG 1 cut(s) 220
NlaIII CATG 2 cut(s) 289, 295
NlaIV GGNNCC 1 cut(s) 84
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 289
PciI ACATGT 1 cut(s) 285
PfeI GAWTC 2 cut(s) 187, 209
PkrI GCNGC 1 cut(s) 301
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
PscI ACATGT 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 308
PspEI GGTNACC 1 cut(s) 328
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 1 cut(s) 84
RsaI GTAC 3 cut(s) 84, 215, 290
RsaNI GTAC 3 cut(s) 83, 214, 289
RseI CAYNNNNRTG 1 cut(s) 290
SatI GCNGC 1 cut(s) 300
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
Sse9I AATT 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 308
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 1 cut(s) 23
TasI AATT 1 cut(s) 96
TatI WGTACW 1 cut(s) 288
TfiI GAWTC 2 cut(s) 187, 209
TscAI CASTG 1 cut(s) 270
TseI GCWGC 1 cut(s) 299
TspDTI ATGAA 2 cut(s) 40, 326
TspRI CASTG 1 cut(s) 270
XagI CCTNNNNNAGG 1 cut(s) 307
XapI RAATTY 1 cut(s) 96
XceI RCATGY 1 cut(s) 289
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.