Rh5AG085500

gamma-irradiation and mitomycin c induced

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001133
Physical Location & Seq
Reverse (-)
7484807 .. 7488708
3902 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG085500.1

Sequence Viewer

Length: 351 bp
ATGTCTGACGTTTGCGTGGCTGGTACTCAAATGGAAAATATGGTTTTTGAAATCGTCAACTCTGATGGTGTTGTTGATGATACTATTCATCATACCGAGAAAAGTGGCCAATTACATATGCTTACAATAATGGCCGGTTCATCATCCATGGAGGAATCCCTTCGATACACATTCAAGCATGGACGCTGTACTGTTCCGGCTATTTTTGTTCCTGAAGTTGAAGAGAGTTTTAACTTTGTAGCTGCTCATTCATCTCATCTAGAACTGCATCTGAATGTGAAGATTGGGCCAGCTTTCACAGAAACTTATCATTATTTATCACAAAAAAAAAAAAAAAAAATCAGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c_3

Protein Analysis

116

Amino Acids

13.0

Weight (kDa)

6.02

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 106, 132
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 2 cut(s) 25, 190
AgsI TTSAA 3 cut(s) 50, 175, 221
AluBI AGCT 2 cut(s) 242, 293
AluI AGCT 2 cut(s) 242, 293
AoxI GGCC 3 cut(s) 106, 132, 287
ApeKI GCWGC 1 cut(s) 242
Asp700I GAANNNNTTC 1 cut(s) 159
AspS9I GGNCC 1 cut(s) 287
BalI TGGCCA 1 cut(s) 108
BbvI GCAGC 1 cut(s) 229
BccI CCATC 1 cut(s) 59
BfaI CTAG 1 cut(s) 260
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 287
BmsI GCATC 1 cut(s) 277
BsaJI CCNNGG 1 cut(s) 147
Bse118I RCCGGY 1 cut(s) 134
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 1 cut(s) 143
BseXI GCAGC 1 cut(s) 229
BshFI GGCC 3 cut(s) 108, 134, 289
BsiSI CCGG 2 cut(s) 135, 197
BsnI GGCC 3 cut(s) 108, 134, 289
Bsp19I CCATGG 1 cut(s) 147
BspANI GGCC 3 cut(s) 108, 134, 289
BsrFI RCCGGY 1 cut(s) 134
BssAI RCCGGY 1 cut(s) 134
BssECI CCNNGG 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 193
Bst6I CTCTTC 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 291
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 1 cut(s) 143
BstV1I GCAGC 1 cut(s) 229
BsuRI GGCC 3 cut(s) 108, 134, 289
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 291
Cfr10I RCCGGY 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 287
CseI GACGC 1 cut(s) 192
Csp6I GTAC 2 cut(s) 24, 189
CviAII CATG 2 cut(s) 148, 179
CviJI RGCY 7 cut(s) 20, 108, 134, 200, 242, 289, 293
CviKI_1 RGCY 7 cut(s) 20, 108, 134, 200, 242, 289, 293
CviQI GTAC 2 cut(s) 24, 189
EaeI YGGCCR 2 cut(s) 106, 132
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 147
Eco57I CTGAAG 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 2 cut(s) 151, 182
FaiI YATR 6 cut(s) 41, 93, 117, 119, 149, 180
FatI CATG 2 cut(s) 147, 178
FauNDI CATATG 1 cut(s) 117
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 1 cut(s) 130
Fsp4HI GCNGC 1 cut(s) 243
FspBI CTAG 1 cut(s) 260
GluI GCNGC 1 cut(s) 243
HaeIII GGCC 3 cut(s) 108, 134, 289
HapII CCGG 2 cut(s) 135, 197
HgaI GACGC 1 cut(s) 192
Hin1II CATG 2 cut(s) 151, 182
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 1 cut(s) 155
HpaII CCGG 2 cut(s) 135, 197
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 3 cut(s) 7, 64, 273
Hpy188III TCNNGA 2 cut(s) 212, 260
Hpy8I GTNNAC 1 cut(s) 58
HpyAV CCTTC 1 cut(s) 170
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 1 cut(s) 268
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 2 cut(s) 151, 182
LpnPI CCDG 5 cut(s) 6, 148, 210, 225, 303
Lsp1109I GCAGC 1 cut(s) 229
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 260
MaeII ACGT 1 cut(s) 9
MboII GAAGA 2 cut(s) 233, 292
MfeI CAATTG 1 cut(s) 346
MlsI TGGCCA 1 cut(s) 108
MluCI AATT 2 cut(s) 110, 346
MluNI TGGCCA 1 cut(s) 108
MnlI CCTC 1 cut(s) 145
Mox20I TGGCCA 1 cut(s) 108
MroXI GAANNNNTTC 1 cut(s) 159
MscI TGGCCA 1 cut(s) 108
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 1 cut(s) 273
Msp20I TGGCCA 1 cut(s) 108
MspI CCGG 2 cut(s) 135, 197
MunI CAATTG 1 cut(s) 346
NcoI CCATGG 1 cut(s) 147
NdeI CATATG 1 cut(s) 117
NlaIII CATG 2 cut(s) 151, 182
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 155
PkrI GCNGC 1 cut(s) 244
PspPI GGNCC 1 cut(s) 287
RsaI GTAC 2 cut(s) 25, 190
RsaNI GTAC 2 cut(s) 24, 189
RseI CAYNNNNRTG 1 cut(s) 273
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 1 cut(s) 243
Sau96I GGNCC 1 cut(s) 287
SetI ASST 3 cut(s) 12, 244, 295
SfaNI GCATC 1 cut(s) 277
SmiMI CAYNNNNRTG 1 cut(s) 273
Sse9I AATT 2 cut(s) 110, 346
SspMI CTAG 1 cut(s) 260
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 1 cut(s) 12
TaqI TCGA 1 cut(s) 163
TasI AATT 2 cut(s) 110, 346
TatI WGTACW 1 cut(s) 188
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TseI GCWGC 1 cut(s) 242
TspDTI ATGAA 3 cut(s) 77, 129, 240
XbaI TCTAGA 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 159
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.