Rh5BG265400

Plant mobile domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001133
Physical Location & Seq
Forward (+)
33186669 .. 33187004
336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG265400.1

Sequence Viewer

Length: 336 bp
ATGTCAAAACTACGTCGTTTTGACGGAAAGAAACAGAAAAAAAAAATTTCCTGCGTCTTCTTCTTCCTTCAGCTCCCGCCTGAGGCGCCGCATCTGCAAAAAGCGACGCTCCGTCGCCTAGGCGACCGCTTTTCCAGCAGCACGTCTAAGTTTGATCCGGCGAGGCTTTTTCCGGTCGCCTCACACCGGAGCACGCCCCGGGCTCGCCTCGGCGACGCATTTGAAAACATTGCTAAATACCATGGCGAGAAGGAAAAGTGGGTTCGCCCAGGTCTGGAGGATGAATTATTGTCATTTGTCATGTGCTTGAGGGCGACTGAGTTAGCTGGATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMD

Protein Analysis

111

Amino Acids

12.78

Weight (kDa)

10.07

Isoelectric Point (pI)

66.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 85
AciI CCGC 3 cut(s) 77, 89, 127
AclWI GGATC 1 cut(s) 149
AcsI RAATTY 1 cut(s) 45
AcuI CTGAAG 1 cut(s) 53
AcyI GRCGYC 1 cut(s) 86
AfiI CCNNNNNNNGG 3 cut(s) 82, 186, 274
AgsI TTSAA 1 cut(s) 224
AhdI GACNNNNNGTC 1 cut(s) 111
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 1 cut(s) 268
AluBI AGCT 2 cut(s) 73, 326
AluI AGCT 2 cut(s) 73, 326
Alw21I GWGCWC 1 cut(s) 194
AlwI GGATC 1 cut(s) 149
Ama87I CYCGRG 1 cut(s) 198
ApeKI GCWGC 1 cut(s) 138
ApoI RAATTY 1 cut(s) 45
AspA2I CCTAGG 1 cut(s) 118
AspLEI GCGC 1 cut(s) 88
AsuC2I CCSGG 2 cut(s) 199, 200
AvaI CYCGRG 1 cut(s) 198
AvrII CCTAGG 1 cut(s) 118
AxyI CCTNAGG 1 cut(s) 81
BanI GGYRCC 1 cut(s) 85
BanII GRGCYC 1 cut(s) 205
BbsI GAAGAC 1 cut(s) 49
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 270
BciVI GTATCC 1 cut(s) 323
BcnI CCSGG 2 cut(s) 199, 200
BfaI CTAG 2 cut(s) 119, 334
BfoI RGCGCY 1 cut(s) 89
BfuI GTATCC 1 cut(s) 323
BisI GCNGC 2 cut(s) 89, 139
BlnI CCTAGG 1 cut(s) 118
BlsI GCNGC 2 cut(s) 90, 140
Bme1390I CCNGG 3 cut(s) 199, 200, 270
BmeRI GACNNNNNGTC 1 cut(s) 111
BmeT110I CYCGRG 1 cut(s) 198
BmgBI CACGTC 1 cut(s) 144
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 3 cut(s) 199, 200, 270
BmsI GCATC 1 cut(s) 100
BpiI GAAGAC 1 cut(s) 49
BpmI CTGGAG 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 328
BpuMI CCSGG 2 cut(s) 199, 200
BsaHI GRCGYC 1 cut(s) 86
BsaJI CCNNGG 6 cut(s) 118, 197, 198, 208, 241, 268
BsaWI WCCGGW 2 cut(s) 172, 186
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 186, 274
Bse21I CCTNAGG 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 6 cut(s) 118, 197, 198, 208, 241, 268
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 3 cut(s) 82, 186, 274
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 72, 309
BseXI GCAGC 1 cut(s) 150
Bsh1285I CGRYCG 2 cut(s) 127, 177
BshNI GGYRCC 1 cut(s) 85
BsiEI CGRYCG 2 cut(s) 127, 177
BsiHKAI GWGCWC 1 cut(s) 194
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 4 cut(s) 158, 173, 187, 199
BslI CCNNNNNNNGG 3 cut(s) 82, 186, 274
BsoBI CYCGRG 1 cut(s) 198
Bsp1286I GDGCHC 2 cut(s) 194, 205
Bsp143I GATC 1 cut(s) 154
Bsp19I CCATGG 1 cut(s) 241
BspACI CCGC 3 cut(s) 77, 89, 127
BspCNI CTCAG 2 cut(s) 73, 310
BspLI GGNNCC 1 cut(s) 87
BspPI GGATC 1 cut(s) 149
BspT107I GGYRCC 1 cut(s) 85
BsrDI GCAATG 1 cut(s) 228
BssECI CCNNGG 6 cut(s) 118, 197, 198, 208, 241, 268
BssMI GATC 1 cut(s) 154
BssNI GRCGYC 1 cut(s) 86
BssT1I CCWWGG 2 cut(s) 118, 241
Bst2UI CCWGG 1 cut(s) 270
BstACI GRCGYC 1 cut(s) 86
BstC8I GCNNGC 2 cut(s) 194, 205
BstDEI CTNAG 3 cut(s) 81, 147, 318
BstDSI CCRYGG 1 cut(s) 241
BstF5I GGATG 1 cut(s) 286
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 1 cut(s) 157
BstMBI GATC 1 cut(s) 154
BstMCI CGRYCG 2 cut(s) 127, 177
BstMWI GCNNNNNNNGC 3 cut(s) 85, 94, 135
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 3 cut(s) 197, 198, 268
BstV1I GCAGC 1 cut(s) 150
BstV2I GAAGAC 1 cut(s) 49
Bsu36I CCTNAGG 1 cut(s) 81
BsuI GTATCC 1 cut(s) 323
BtgI CCRYGG 1 cut(s) 241
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 1 cut(s) 286
Cac8I GCNNGC 2 cut(s) 194, 205
CfoI GCGC 1 cut(s) 88
Cfr9I CCCGGG 1 cut(s) 198
CseI GACGC 3 cut(s) 43, 115, 224
CviAII CATG 2 cut(s) 242, 301
CviJI RGCY 4 cut(s) 73, 166, 203, 326
CviKI_1 RGCY 4 cut(s) 73, 166, 203, 326
DdeI CTNAG 3 cut(s) 81, 147, 318
DinI GGCGCC 1 cut(s) 87
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
DriI GACNNNNNGTC 1 cut(s) 111
Eam1105I GACNNNNNGTC 1 cut(s) 111
Eco130I CCWWGG 2 cut(s) 118, 241
Eco24I GRGCYC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 198
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 2 cut(s) 118, 241
EcoT38I GRGCYC 1 cut(s) 205
EgeI GGCGCC 1 cut(s) 87
EheI GGCGCC 1 cut(s) 87
ErhI CCWWGG 2 cut(s) 118, 241
FaeI CATG 2 cut(s) 245, 304
FaiI YATR 2 cut(s) 243, 302
FatI CATG 2 cut(s) 241, 300
FauI CCCGC 1 cut(s) 84
Fnu4HI GCNGC 2 cut(s) 89, 139
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 2 cut(s) 89, 139
FspBI CTAG 2 cut(s) 119, 334
GlaI GCGC 1 cut(s) 87
GluI GCNGC 2 cut(s) 89, 139
GsuI CTGGAG 1 cut(s) 296
HaeII RGCGCY 1 cut(s) 89
HapII CCGG 4 cut(s) 158, 173, 187, 199
HgaI GACGC 3 cut(s) 43, 115, 224
HhaI GCGC 1 cut(s) 88
Hin1I GRCGYC 1 cut(s) 86
Hin1II CATG 2 cut(s) 245, 304
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HpaII CCGG 4 cut(s) 158, 173, 187, 199
Hpy188III TCNNGA 1 cut(s) 275
Hpy99I CGWCG 4 cut(s) 18, 109, 117, 218
HpyAV CCTTC 2 cut(s) 77, 244
HpyCH4IV ACGT 2 cut(s) 13, 143
HpyCH4V TGCA 1 cut(s) 97
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 94, 135
HpyF3I CTNAG 3 cut(s) 81, 147, 318
HpySE526I ACGT 2 cut(s) 13, 143
Hsp92I GRCGYC 1 cut(s) 86
Hsp92II CATG 2 cut(s) 245, 304
HspAI GCGC 1 cut(s) 86
KasI GGCGCC 1 cut(s) 85
Kzo9I GATC 1 cut(s) 154
LmnI GCTCC 3 cut(s) 78, 114, 189
Lsp1109I GCAGC 1 cut(s) 150
LweI GCATC 1 cut(s) 100
MaeI CTAG 2 cut(s) 119, 334
MaeII ACGT 2 cut(s) 13, 143
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MboII GAAGA 3 cut(s) 49, 52, 55
MhlI GDGCHC 2 cut(s) 194, 205
MluCI AATT 2 cut(s) 45, 284
Mly113I GGCGCC 1 cut(s) 86
MnlI CCTC 6 cut(s) 76, 156, 190, 218, 271, 303
MspI CCGG 4 cut(s) 158, 173, 187, 199
MspR9I CCNGG 3 cut(s) 199, 200, 270
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 3 cut(s) 85, 94, 135
NarI GGCGCC 1 cut(s) 86
NciI CCSGG 2 cut(s) 199, 200
NcoI CCATGG 1 cut(s) 241
NdeII GATC 1 cut(s) 154
NlaIII CATG 2 cut(s) 245, 304
NlaIV GGNNCC 1 cut(s) 87
NmeAIII GCCGAG 1 cut(s) 189
PkrI GCNGC 2 cut(s) 90, 140
PluTI GGCGCC 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 1 cut(s) 87
SatI GCNGC 2 cut(s) 89, 139
Sau3AI GATC 1 cut(s) 154
ScrFI CCNGG 3 cut(s) 199, 200, 270
SduI GDGCHC 2 cut(s) 194, 205
SetI ASST 5 cut(s) 16, 75, 146, 274, 328
SfaNI GCATC 1 cut(s) 100
SfoI GGCGCC 1 cut(s) 87
SmaI CCCGGG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 2 cut(s) 45, 284
SsiI CCGC 3 cut(s) 77, 89, 127
SspDI GGCGCC 1 cut(s) 85
SspMI CTAG 2 cut(s) 119, 334
StyD4I CCNGG 3 cut(s) 197, 198, 268
StyI CCWWGG 2 cut(s) 118, 241
TaiI ACGT 2 cut(s) 16, 146
TasI AATT 2 cut(s) 45, 284
TauI GCSGC 1 cut(s) 91
TseI GCWGC 1 cut(s) 138
TspDTI ATGAA 1 cut(s) 297
TspGWI ACGGA 2 cut(s) 39, 101
TspMI CCCGGG 1 cut(s) 198
XapI RAATTY 1 cut(s) 45
XmaI CCCGGG 1 cut(s) 198
XmaJI CCTAGG 1 cut(s) 118
XspI CTAG 2 cut(s) 119, 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.