Rh6AG200300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001133
Physical Location & Seq
Reverse (-)
35980217 .. 35980601
385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG200300.1

Sequence Viewer

Length: 264 bp
ATGACCCTCGCGTCTTCTTCTTCCTCACACTGCCGCCTCAGGCGCCGCTTCTGCAAAAAAGCGACGCTCCATCGCCTGGGCGACCGCTTTTCCAGCAGCACGTCTCAGTTTGATCCAGCGAGGCTTTTTCTGGTCGCCTCACACCGGAGCGCGCCCCGGGCTCGCCTCGGCGACGCCTTTGAAAACACTGATTTGGAGGAACTACTGAGGGTAAGCAAGGGGAAGCAACACTTCAACATTGATCTCTCACCCTATCGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

87

Amino Acids

9.95

Weight (kDa)

10.47

Isoelectric Point (pI)

61.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AccB1I GGYRCC 1 cut(s) 42
AccB7I CCANNNNNTGG 1 cut(s) 76
AccII CGCG 2 cut(s) 11, 152
AciI CCGC 3 cut(s) 34, 46, 85
AclWI GGATC 1 cut(s) 107
AcyI GRCGYC 2 cut(s) 43, 174
AfiI CCNNNNNNNGG 2 cut(s) 76, 144
AgsI TTSAA 2 cut(s) 182, 235
AjiI CACGTC 1 cut(s) 102
AjnI CCWGG 1 cut(s) 75
Alw26I GTCTC 1 cut(s) 108
AlwI GGATC 1 cut(s) 107
Ama87I CYCGRG 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 96
AspLEI GCGC 3 cut(s) 45, 152, 154
AsuC2I CCSGG 2 cut(s) 157, 158
AsuHPI GGTGA 1 cut(s) 240
AvaI CYCGRG 1 cut(s) 156
AxyI CCTNAGG 1 cut(s) 38
BanI GGYRCC 1 cut(s) 42
BanII GRGCYC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 6
BbvI GCAGC 1 cut(s) 108
BccI CCATC 1 cut(s) 78
BciT130I CCWGG 1 cut(s) 77
BcnI CCSGG 2 cut(s) 157, 158
BcoDI GTCTC 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 46
BisI GCNGC 3 cut(s) 34, 46, 97
BlsI GCNGC 3 cut(s) 35, 47, 98
Bme1390I CCNGG 3 cut(s) 77, 157, 158
BmeT110I CYCGRG 1 cut(s) 156
BmgBI CACGTC 1 cut(s) 102
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 3 cut(s) 77, 157, 158
BpiI GAAGAC 1 cut(s) 6
BpuMI CCSGG 2 cut(s) 157, 158
BsaHI GRCGYC 2 cut(s) 43, 174
BsaJI CCNNGG 4 cut(s) 76, 155, 156, 166
BsaWI WCCGGW 1 cut(s) 144
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 144
Bse21I CCTNAGG 1 cut(s) 38
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 4 cut(s) 76, 155, 156, 166
BseLI CCNNNNNNNGG 2 cut(s) 76, 144
BseMII CTCAG 3 cut(s) 52, 119, 197
BsePI GCGCGC 1 cut(s) 150
BseXI GCAGC 1 cut(s) 108
Bsh1236I CGCG 2 cut(s) 11, 152
Bsh1285I CGRYCG 1 cut(s) 85
BshNI GGYRCC 1 cut(s) 42
BsiEI CGRYCG 1 cut(s) 85
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 2 cut(s) 145, 157
BslI CCNNNNNNNGG 2 cut(s) 76, 144
BsmAI GTCTC 1 cut(s) 108
BsmBI CGTCTC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 156
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 2 cut(s) 112, 241
BspACI CCGC 3 cut(s) 34, 46, 85
BspCNI CTCAG 3 cut(s) 51, 118, 198
BspFNI CGCG 2 cut(s) 11, 152
BspLI GGNNCC 1 cut(s) 44
BspPI GGATC 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 42
BssECI CCNNGG 4 cut(s) 76, 155, 156, 166
BssHII GCGCGC 1 cut(s) 150
BssMI GATC 2 cut(s) 112, 241
BssNI GRCGYC 2 cut(s) 43, 174
Bst2UI CCWGG 1 cut(s) 77
BstACI GRCGYC 2 cut(s) 43, 174
BstC8I GCNNGC 2 cut(s) 152, 163
BstDEI CTNAG 3 cut(s) 38, 105, 206
BstFNI CGCG 2 cut(s) 11, 152
BstH2I RGCGCY 1 cut(s) 46
BstHHI GCGC 3 cut(s) 45, 152, 154
BstKTI GATC 2 cut(s) 115, 244
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 2 cut(s) 112, 241
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 4 cut(s) 42, 51, 93, 158
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 3 cut(s) 75, 155, 156
BstUI CGCG 2 cut(s) 11, 152
BstV1I GCAGC 1 cut(s) 108
BstV2I GAAGAC 1 cut(s) 6
Bsu36I CCTNAGG 1 cut(s) 38
BtgZI GCGATG 1 cut(s) 56
BtrI CACGTC 1 cut(s) 102
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 2 cut(s) 28, 186
Cac8I GCNNGC 2 cut(s) 152, 163
CfoI GCGC 3 cut(s) 45, 152, 154
Cfr9I CCCGGG 1 cut(s) 156
CseI GACGC 2 cut(s) 73, 182
CviJI RGCY 2 cut(s) 124, 161
CviKI_1 RGCY 2 cut(s) 124, 161
DdeI CTNAG 3 cut(s) 38, 105, 206
DinI GGCGCC 1 cut(s) 44
DpnI GATC 2 cut(s) 114, 243
DpnII GATC 2 cut(s) 112, 241
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 163
Eco81I CCTNAGG 1 cut(s) 38
Eco88I CYCGRG 1 cut(s) 156
EcoRII CCWGG 1 cut(s) 75
EcoT38I GRGCYC 1 cut(s) 163
EgeI GGCGCC 1 cut(s) 44
EheI GGCGCC 1 cut(s) 44
Esp3I CGTCTC 1 cut(s) 108
FalI AAGNNNNNCTT 2 cut(s) 215, 247
Fnu4HI GCNGC 3 cut(s) 34, 46, 97
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 3 cut(s) 34, 46, 97
GlaI GCGC 3 cut(s) 44, 151, 153
GluI GCNGC 3 cut(s) 34, 46, 97
HaeII RGCGCY 1 cut(s) 46
HapII CCGG 2 cut(s) 145, 157
HgaI GACGC 2 cut(s) 73, 182
HhaI GCGC 3 cut(s) 45, 152, 154
Hin1I GRCGYC 2 cut(s) 43, 174
Hin6I GCGC 3 cut(s) 43, 150, 152
HinP1I GCGC 3 cut(s) 43, 150, 152
HpaII CCGG 2 cut(s) 145, 157
HphI GGTGA 1 cut(s) 240
Hpy188III TCNNGA 1 cut(s) 257
Hpy99I CGWCG 2 cut(s) 67, 176
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 1 cut(s) 54
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 51, 93, 158
HpyF3I CTNAG 3 cut(s) 38, 105, 206
HpySE526I ACGT 1 cut(s) 101
Hsp92I GRCGYC 2 cut(s) 43, 174
HspAI GCGC 3 cut(s) 43, 150, 152
KasI GGCGCC 1 cut(s) 42
Kzo9I GATC 2 cut(s) 112, 241
LmnI GCTCC 2 cut(s) 72, 147
LpnPI CCDG 8 cut(s) 25, 62, 89, 106, 116, 129, 158, 170
Lsp1109I GCAGC 1 cut(s) 108
MaeII ACGT 1 cut(s) 101
MalI GATC 2 cut(s) 114, 243
MboI GATC 2 cut(s) 112, 241
MboII GAAGA 3 cut(s) 6, 9, 12
MhlI GDGCHC 1 cut(s) 163
Mly113I GGCGCC 1 cut(s) 43
MnlI CCTC 8 cut(s) 17, 34, 47, 114, 148, 176, 190, 201
MspI CCGG 2 cut(s) 145, 157
MspR9I CCNGG 3 cut(s) 77, 157, 158
MvaI CCWGG 1 cut(s) 77
MvnI CGCG 2 cut(s) 11, 152
MwoI GCNNNNNNNGC 4 cut(s) 42, 51, 93, 158
NarI GGCGCC 1 cut(s) 43
NciI CCSGG 2 cut(s) 157, 158
NdeII GATC 2 cut(s) 112, 241
NlaIV GGNNCC 1 cut(s) 44
NmeAIII GCCGAG 1 cut(s) 147
PauI GCGCGC 1 cut(s) 150
PflMI CCANNNNNTGG 1 cut(s) 76
PkrI GCNGC 3 cut(s) 35, 47, 98
PluTI GGCGCC 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 44
PteI GCGCGC 1 cut(s) 150
SatI GCNGC 3 cut(s) 34, 46, 97
Sau3AI GATC 2 cut(s) 112, 241
ScrFI CCNGG 3 cut(s) 77, 157, 158
SduI GDGCHC 1 cut(s) 163
SetI ASST 1 cut(s) 104
SfoI GGCGCC 1 cut(s) 44
SmaI CCCGGG 1 cut(s) 158
SsiI CCGC 3 cut(s) 34, 46, 85
SspDI GGCGCC 1 cut(s) 42
StyD4I CCNGG 3 cut(s) 75, 155, 156
TaiI ACGT 1 cut(s) 104
TauI GCSGC 2 cut(s) 36, 48
TscAI CASTG 2 cut(s) 35, 193
TseI GCWGC 1 cut(s) 96
TspMI CCCGGG 1 cut(s) 156
TspRI CASTG 2 cut(s) 35, 193
Van91I CCANNNNNTGG 1 cut(s) 76
XmaI CCCGGG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.