FvH4_7g31684

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003051
Physical Location & Seq
Reverse (-)
22871202 .. 22872800
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31684.t1

Sequence Viewer

Length: 387 bp
ATGGCCGGCGCCGGCATCCACCCCTACCACCAGCAATGGGCCCCGGCGGCAGCCCCTCCTCCTCCCCCGGCCGTCGCCGCTGCCATTCTTAACTCTGAGTGGGTACTTGTCACGTTCTTCTCTATTATGTCTTTATATAGTTTCATTCAAATGTTGAAAGACAAACCTGTAGTAATAGATGAATCTCTTACCGTTCTTGAGGATAATCGTGTTCCTCTGGATGCAGCTTCTCGACCAAGCGATATAGGGATTCTGGGTACAGAGTTTGATACTCTTCATGATTGTGGTGGTGATGATGAGAGATGTGTGTGGGGTTGGACACAAGAAGAGGAGAGGATTCCTATGAAGGACCCTATTGACGAGTTTCATAAGTACATTGGTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

129

Amino Acids

14.16

Weight (kDa)

4.42

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AciI CCGC 2 cut(s) 47, 78
AcoI YGGCCR 2 cut(s) 3, 69
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 3 cut(s) 105, 259, 374
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 2 cut(s) 149, 157
AluBI AGCT 1 cut(s) 227
AluI AGCT 1 cut(s) 227
AoxI GGCC 3 cut(s) 3, 39, 69
ApaI GGGCCC 1 cut(s) 43
ApeKI GCWGC 3 cut(s) 50, 80, 224
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 3 cut(s) 39, 40, 349
AsuC2I CCSGG 2 cut(s) 44, 68
AsuHPI GGTGA 1 cut(s) 302
AvaII GGWCC 1 cut(s) 349
BaeGI GKGCMC 1 cut(s) 43
BanI GGYRCC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 43
BbvI GCAGC 3 cut(s) 62, 67, 236
BceAI ACGGC 1 cut(s) 56
BcnI CCSGG 2 cut(s) 44, 68
BfmI CTRYAG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 12
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 5 cut(s) 48, 51, 78, 81, 225
BlsI GCNGC 5 cut(s) 49, 52, 79, 82, 226
Bme1390I CCNGG 2 cut(s) 44, 68
Bme18I GGWCC 1 cut(s) 349
BmgT120I GGNCC 3 cut(s) 39, 40, 349
BmiI GGNNCC 4 cut(s) 10, 41, 42, 351
BmrFI CCNGG 2 cut(s) 44, 68
BmsI GCATC 2 cut(s) 24, 211
BpuEI CTTGAG 1 cut(s) 218
BpuMI CCSGG 2 cut(s) 44, 68
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 2 cut(s) 42, 66
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse118I RCCGGY 2 cut(s) 5, 11
Bse3DI GCAATG 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 42, 66
BseGI GGATG 2 cut(s) 15, 226
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 1 cut(s) 87
BseRI GAGGAG 3 cut(s) 48, 51, 344
BseSI GKGCMC 1 cut(s) 43
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 3 cut(s) 62, 67, 236
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 3 cut(s) 5, 41, 71
BshNI GGYRCC 1 cut(s) 8
BsiEI CGRYCG 1 cut(s) 72
BsiSI CCGG 4 cut(s) 6, 12, 44, 68
BslI CCNNNNNNNGG 1 cut(s) 37
BsnI GGCC 3 cut(s) 5, 41, 71
Bsp120I GGGCCC 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 43
BspACI CCGC 2 cut(s) 47, 78
BspANI GGCC 3 cut(s) 5, 41, 71
BspCNI CTCAG 1 cut(s) 88
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 4 cut(s) 10, 41, 42, 351
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 41
BsrFI RCCGGY 2 cut(s) 5, 11
BssAI RCCGGY 2 cut(s) 5, 11
BssECI CCNNGG 2 cut(s) 42, 66
BssNI GRCGYC 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 193
Bst6I CTCTTC 2 cut(s) 279, 321
BstACI GRCGYC 1 cut(s) 9
BstC8I GCNNGC 2 cut(s) 7, 13
BstDEI CTNAG 1 cut(s) 96
BstF5I GGATG 2 cut(s) 15, 226
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstMCI CGRYCG 1 cut(s) 72
BstMWI GCNNNNNNNGC 2 cut(s) 47, 77
BstSCI CCNGG 2 cut(s) 42, 66
BstSFI CTRYAG 1 cut(s) 168
BstSLI GKGCMC 1 cut(s) 43
BstV1I GCAGC 3 cut(s) 62, 67, 236
BstZI CGGCCG 1 cut(s) 69
BsuRI GGCC 3 cut(s) 5, 41, 71
BtsCI GGATG 2 cut(s) 15, 226
Cac8I GCNNGC 2 cut(s) 7, 13
CciI TCATGA 1 cut(s) 277
CfoI GCGC 1 cut(s) 11
Cfr10I RCCGGY 2 cut(s) 5, 11
Cfr13I GGNCC 3 cut(s) 39, 40, 349
Csp6I GTAC 3 cut(s) 104, 258, 373
CviAII CATG 1 cut(s) 278
CviJI RGCY 5 cut(s) 5, 41, 53, 71, 227
CviKI_1 RGCY 5 cut(s) 5, 41, 53, 71, 227
CviQI GTAC 3 cut(s) 104, 258, 373
DdeI CTNAG 1 cut(s) 96
DinI GGCGCC 1 cut(s) 10
EaeI YGGCCR 2 cut(s) 3, 69
EagI CGGCCG 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 279, 321
EarI CTCTTC 2 cut(s) 279, 321
EclXI CGGCCG 1 cut(s) 69
Eco24I GRGCYC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 349
Eco52I CGGCCG 1 cut(s) 69
EcoO109I RGGNCCY 2 cut(s) 40, 349
EcoT38I GRGCYC 1 cut(s) 43
EgeI GGCGCC 1 cut(s) 10
EheI GGCGCC 1 cut(s) 10
FaeI CATG 1 cut(s) 281
FaiI YATR 7 cut(s) 128, 136, 138, 245, 279, 344, 369
FatI CATG 1 cut(s) 277
Fnu4HI GCNGC 5 cut(s) 48, 51, 78, 81, 225
FokI GGATG 2 cut(s) 2, 233
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 5 cut(s) 48, 51, 78, 81, 225
GlaI GCGC 1 cut(s) 10
GluI GCNGC 5 cut(s) 48, 51, 78, 81, 225
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 3 cut(s) 5, 41, 71
HapII CCGG 4 cut(s) 6, 12, 44, 68
HhaI GCGC 1 cut(s) 11
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 1 cut(s) 281
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 3 cut(s) 182, 250, 337
HpaII CCGG 4 cut(s) 6, 12, 44, 68
HphI GGTGA 1 cut(s) 302
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 4 cut(s) 197, 218, 231, 278
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 77
HpyF3I CTNAG 1 cut(s) 96
HpySE526I ACGT 1 cut(s) 113
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 1 cut(s) 281
HspAI GCGC 1 cut(s) 9
KasI GGCGCC 1 cut(s) 8
KroI GCCGGC 2 cut(s) 5, 11
KroNI GCCGGC 2 cut(s) 7, 13
LpnPI CCDG 8 cut(s) 19, 25, 44, 57, 81, 180, 203, 239
Lsp1109I GCAGC 3 cut(s) 62, 67, 236
LweI GCATC 2 cut(s) 24, 211
MaeII ACGT 1 cut(s) 113
MaeIII GTNAC 1 cut(s) 109
MboII GAAGA 3 cut(s) 109, 266, 338
MhlI GDGCHC 1 cut(s) 43
Mly113I GGCGCC 1 cut(s) 9
MmeI TCCRAC 1 cut(s) 296
MnlI CCTC 7 cut(s) 66, 69, 72, 193, 225, 322, 327
MroNI GCCGGC 2 cut(s) 5, 11
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 2 cut(s) 149, 282
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 4 cut(s) 6, 12, 44, 68
MspR9I CCNGG 2 cut(s) 44, 68
MwoI GCNNNNNNNGC 2 cut(s) 47, 77
NaeI GCCGGC 2 cut(s) 7, 13
NarI GGCGCC 1 cut(s) 9
NciI CCSGG 2 cut(s) 44, 68
NgoMIV GCCGGC 2 cut(s) 5, 11
NlaIII CATG 1 cut(s) 281
NlaIV GGNNCC 4 cut(s) 10, 41, 42, 351
NmuCI GTSAC 1 cut(s) 109
PagI TCATGA 1 cut(s) 277
PdiI GCCGGC 2 cut(s) 7, 13
PfeI GAWTC 3 cut(s) 182, 250, 337
PkrI GCNGC 5 cut(s) 49, 52, 79, 82, 226
PluTI GGCGCC 1 cut(s) 12
PpuMI RGGWCCY 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 349
PspN4I GGNNCC 4 cut(s) 10, 41, 42, 351
PspOMI GGGCCC 1 cut(s) 39
PspPI GGNCC 3 cut(s) 39, 40, 349
PspPPI RGGWCCY 1 cut(s) 349
RsaI GTAC 3 cut(s) 105, 259, 374
RsaNI GTAC 3 cut(s) 104, 258, 373
RseI CAYNNNNRTG 2 cut(s) 149, 282
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 5 cut(s) 48, 51, 78, 81, 225
Sau96I GGNCC 3 cut(s) 39, 40, 349
ScrFI CCNGG 2 cut(s) 44, 68
SduI GDGCHC 1 cut(s) 43
SetI ASST 3 cut(s) 116, 169, 229
SfaNI GCATC 2 cut(s) 24, 211
SfcI CTRYAG 1 cut(s) 168
SfoI GGCGCC 1 cut(s) 10
SinI GGWCC 1 cut(s) 349
SmiMI CAYNNNNRTG 2 cut(s) 149, 282
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SsiI CCGC 2 cut(s) 47, 78
SspDI GGCGCC 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 42, 66
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 1 cut(s) 116
TaqI TCGA 1 cut(s) 232
TatI WGTACW 1 cut(s) 372
TauI GCSGC 2 cut(s) 50, 80
TfiI GAWTC 3 cut(s) 182, 250, 337
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 3 cut(s) 50, 80, 224
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 5 cut(s) 133, 195, 266, 356, 359
VpaK11BI GGWCC 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.