Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0003051

Physical Location & Seq

Genomic Coordinates Reverse (-)

69071764 .. 69080710

Gene Structure 8947 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG491500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1452 bp

ATGGCTCCACCACCTCCTCCATATGATCCATATGGTTATCCTGTTGCTCCAGTACCAATGCCCCCTTCTGCTCCTGTACCAGCACCCAGCAGTTATGTACCTGTTCTGAACACAAAAGATAATCCACCATGCAATACCTTATTTATTGGCAATCTAGGAGAGAGCATTAACGAGGAAGAACTGAGGGGCCTATTCAGCTCACAACCTGGATTTAAGCAAATGAAGATCTTGCGACAGGAAAGGCATACTGTTTGCTTCATTGAGTTTGAAGATGTAAACAGTGCCACTGCTGTACACCACAATTTTCAAGGTCAAGGTTTTGCAGCTGCCCTTTTGTTTCAGGGTTTTATTCGTAACGAGCATTTTGATCCAAAAAAATGGATTATACCTGGAGACTTTTCTGTACCTTGTTTATTAAAGGAGGAGTTGCTTTCTAGTGAAAGAAAAATCTACATCAGGGGAACAAGAAAGATAAAAAGAAAGCGTATTGCTGATGTTGTTGAGGCACTTATTGGTGCATTCCTTATTACCGGTGGTGAAATGGCTGCTGTGCACTTCATGAATTGGGTTGGTATAAAGGAACTTTCCGTTGCAGCCAGAGAAAAGCTTATTAATGTCAAACATTTAGAGAAGCAACTAAACTACTCATTTCGTGATCCTTCTCTTCTAGTGGAAGCACTAACTCGTGGTTCTTACATTCTCCCTGAGATCCCAGGATGTTTTCAGTTATCTGTACAACAAATATCCTGGGATGTCCCAGGAGTCTTAACTGATATGAGATCTGCTTCGGTAAATAATGATTGTTTTGCCCGAGCGGCTGTGAAGGCTGAATTGCACAAGCACATTCTCCATGCTTCAAAGAAACTCCACAAGGATATAGTTCACACAGTTGATAACTTTAAGAAGTTACCTACACAATCAACTTTTGGATGGGAATCTGAGACTTCGTTCCCCAGGGTAGTAGGAGATATTATAGAGTCTCTTGGAGGGGCAATTTTTGTTGATTCTGGATACGATAAGAATGTTGTATTTCAGAGTATACGTCCTCTGTTAGAGCCCTTGATTACTCCAGAAATGATAACTCTCCATCCTGATAGAGAGCTGAATGAACTTTGTTCAAAAATGCATTATGACAGGAAGAAATCAATATCATTCAAAAATGATATGGCTACCATTACTATTGAGGTTGAAGGTAATGGGGTCACATACAAGCATACATCAACAGCTCTTGATAAGTGGACGGCAAGTAAATTAGCAAGCAAAGAAGTTTTGAGGTCCTTGAAAGAGAGTGCTCATTTATCAAAAGACGCTCTCCCATCTTCAAACAACCCCTTCACCACTCCACAACTCGTTTCCTTCACCAAGTCGACTCAGGAAATGGTTCTAGCCAGTGGAGCAGCAGCAGCAGCTCCCCCAACAGGAAGTGCCGCAGCTGCTGCAAGCATGCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

483

Amino Acids

53.38

Weight (kDa)

8.1

Isoelectric Point (pI)

42.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	47 - 106	1.9e-09	RNA recognition motif
Ribonuclease_3	PF00636	253 - 337	6.3e-08	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003051)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21215 AT3G21215 AT3G21215 AT3G21215
fragaria_vesca	FvH4_3g14953 FvH4_3g16432 FvH4_4g15800 FvH4_4g32761 FvH4_5g34521 FvH4_6g16241 FvH4_6g37450 FvH4_6g45392 FvH4_7g15921 FvH4_7g31684
malus_domestica	MD09G1158000.v1.1 MD17G1144000.v1.1
prunus_persica	Prupe.3G168000_v2.0.a1
pyrus_communis	pycom09g07680 pycom17g13730
rosa_chinensis	RchiOBHm_Chr2g0150271 RchiOBHm_Chr2g0150291
rosa_laevigata	RLG00000020483
rosa_multiflora	Rmu_sc0001169.1_g000003 Rmu_sc0020705.1_g000001 Rmu_sc0024000.1_g000001 Rmu_sc0038093.1_g000001 Rmu_sc0040821.1_g000001 Rmu_ssc0000020.1_g000001
rosa_roxburghii	Rroxscaffold_2G00097320 Rroxscaffold_2G00097330
rosa_rugosa	Rorug02G0420200 Rorug02G0420300
rosa_samantha	Rh2AG479200 Rh2BG491500 Rh2CG465400 Rh2DG500300
rosa_wichuraiana	Rw2G038980 Rw2G039110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	815
AccI	GTMKAC	2 cut(s)	1039, 1367
AciI	CCGC	2 cut(s)	815, 1428
AclWI	GGATC	4 cut(s)	20, 362, 650, 703
AfaI	GTAC	6 cut(s)	54, 78, 99, 294, 405, 735
AfiI	CCNNNNNNNGG	1 cut(s)	1418
AgeI	ACCGGT	1 cut(s)	530
AgsI	TTSAA	8 cut(s)	269, 308, 858, 1119, 1156, 1190, 1282, 1323
AjnI	CCWGG	6 cut(s)	205, 388, 712, 746, 757, 953
AjuI	GAANNNNNNNTTGG	2 cut(s)	49, 81
AluBI	AGCT	7 cut(s)	198, 326, 607, 1102, 1226, 1409, 1433
AluI	AGCT	7 cut(s)	198, 326, 607, 1102, 1226, 1409, 1433
Alw21I	GWGCWC	2 cut(s)	555, 1294
Alw26I	GTCTC	3 cut(s)	387, 935, 984
Alw44I	GTGCAC	1 cut(s)	551
AlwI	GGATC	4 cut(s)	20, 362, 650, 703
AlwNI	CAGNNNCTG	1 cut(s)	1436
Ama87I	CYCGRG	1 cut(s)	810
AoxI	GGCC	1 cut(s)	187
ApaLI	GTGCAC	1 cut(s)	551
AseI	ATTAAT	1 cut(s)	612
AsiGI	ACCGGT	1 cut(s)	530
Asp700I	GAANNNNTTC	1 cut(s)	1380
AspLEI	GCGC	1 cut(s)	1449
AspS9I	GGNCC	2 cut(s)	187, 1275
AsuHPI	GGTGA	3 cut(s)	548, 1327, 1351
AvaI	CYCGRG	1 cut(s)	810
AvaII	GGWCC	1 cut(s)	1275
BaeGI	GKGCMC	1 cut(s)	555
BanII	GRGCYC	1 cut(s)	1059
BauI	CACGAG	1 cut(s)	684
Bbv12I	GWGCWC	2 cut(s)	555, 1294
BccI	CCATC	3 cut(s)	924, 1095, 1324
BceAI	ACGGC	1 cut(s)	1257
BciT130I	CCWGG	6 cut(s)	207, 390, 714, 748, 759, 955
BciVI	GTATCC	1 cut(s)	1004
BcoDI	GTCTC	3 cut(s)	387, 935, 984
BfaI	CTAG	4 cut(s)	155, 435, 668, 1385
BfuI	GTATCC	1 cut(s)	1004
BglI	GCCNNNNNGGC	1 cut(s)	815
BglII	AGATCT	2 cut(s)	225, 779
Bme1390I	CCNGG	6 cut(s)	207, 390, 714, 748, 759, 955
Bme18I	GGWCC	1 cut(s)	1275
BmeT110I	CYCGRG	1 cut(s)	810
BmgT120I	GGNCC	2 cut(s)	187, 1275
BmiI	GGNNCC	2 cut(s)	6, 188
BmrFI	CCNGG	6 cut(s)	207, 390, 714, 748, 759, 955
BpmI	CTGGAG	3 cut(s)	33, 411, 1053
BsaBI	GATNNNNATC	1 cut(s)	934
BsaJI	CCNNGG	5 cut(s)	712, 747, 757, 953, 954
BsaWI	WCCGGW	1 cut(s)	530
Bsc4I	CCNNNNNNNGG	1 cut(s)	1418
Bse118I	RCCGGY	1 cut(s)	530
Bse1I	ACTGG	2 cut(s)	50, 1389
Bse8I	GATNNNNATC	1 cut(s)	934
BseBI	CCWGG	6 cut(s)	207, 390, 714, 748, 759, 955
BseDI	CCNNGG	5 cut(s)	712, 747, 757, 953, 954
BseGI	GGATG	4 cut(s)	722, 757, 935, 1087
BseJI	GATNNNNATC	1 cut(s)	934
BseLI	CCNNNNNNNGG	1 cut(s)	1418
BseMII	CTCAG	4 cut(s)	173, 696, 930, 1385
BseNI	ACTGG	2 cut(s)	50, 1389
BseRI	GAGGAG	2 cut(s)	6, 437
BseSI	GKGCMC	1 cut(s)	555
BseYI	CCCAGC	1 cut(s)	86
BshFI	GGCC	1 cut(s)	189
BshTI	ACCGGT	1 cut(s)	530
BsiHKAI	GWGCWC	2 cut(s)	555, 1294
BsiHKCI	CYCGRG	1 cut(s)	810
BsiSI	CCGG	1 cut(s)	531
BslFI	GGGAC	1 cut(s)	740
BslI	CCNNNNNNNGG	1 cut(s)	1418
BsmAI	GTCTC	3 cut(s)	387, 935, 984
BsmFI	GGGAC	1 cut(s)	740
BsmI	GAATGC	1 cut(s)	518
BsnI	GGCC	1 cut(s)	189
BsoBI	CYCGRG	1 cut(s)	810
Bsp1286I	GDGCHC	3 cut(s)	555, 1059, 1294
Bsp1407I	TGTACA	2 cut(s)	292, 733
Bsp143I	GATC	6 cut(s)	25, 225, 367, 655, 708, 779
BspACI	CCGC	2 cut(s)	815, 1428
BspANI	GGCC	1 cut(s)	189
BspCNI	CTCAG	4 cut(s)	174, 697, 931, 1384
BspHI	TCATGA	1 cut(s)	558
BspLI	GGNNCC	2 cut(s)	6, 188
BspPI	GGATC	4 cut(s)	20, 362, 650, 703
BsrBI	CCGCTC	1 cut(s)	815
BsrFI	RCCGGY	1 cut(s)	530
BsrGI	TGTACA	2 cut(s)	292, 733
BsrI	ACTGG	2 cut(s)	50, 1389
BssAI	RCCGGY	1 cut(s)	530
BssECI	CCNNGG	5 cut(s)	712, 747, 757, 953, 954
BssMI	GATC	6 cut(s)	25, 225, 367, 655, 708, 779
BssNAI	GTATAC	1 cut(s)	1040
BssSI	CACGAG	1 cut(s)	684
Bst1107I	GTATAC	1 cut(s)	1040
Bst2BI	CACGAG	1 cut(s)	684
Bst2UI	CCWGG	6 cut(s)	207, 390, 714, 748, 759, 955
Bst4CI	ACNGT	3 cut(s)	250, 281, 889
Bst6I	CTCTTC	1 cut(s)	669
BstAPI	GCANNNNNTGC	1 cut(s)	1436
BstAUI	TGTACA	2 cut(s)	292, 733
BstC8I	GCNNGC	3 cut(s)	1258, 1441, 1445
BstDEI	CTNAG	4 cut(s)	182, 705, 939, 1371
BstF5I	GGATG	4 cut(s)	722, 757, 935, 1087
BstHHI	GCGC	1 cut(s)	1449
BstKTI	GATC	6 cut(s)	28, 228, 370, 658, 711, 782
BstMAI	GTCTC	3 cut(s)	387, 935, 984
BstMBI	GATC	6 cut(s)	25, 225, 367, 655, 708, 779
BstMWI	GCNNNNNNNGC	8 cut(s)	195, 815, 824, 1394, 1403, 1406, 1433, 1436
BstNI	CCWGG	6 cut(s)	207, 390, 714, 748, 759, 955
BstNSI	RCATGY	1 cut(s)	1447
BstSCI	CCNGG	6 cut(s)	205, 388, 712, 746, 757, 953
BstSLI	GKGCMC	1 cut(s)	555
BstX2I	RGATCY	3 cut(s)	225, 708, 779
BstXI	CCANNNNNNTGG	1 cut(s)	378
BstYI	RGATCY	3 cut(s)	225, 708, 779
BstZ17I	GTATAC	1 cut(s)	1040
BsuI	GTATCC	1 cut(s)	1004
BsuRI	GGCC	1 cut(s)	189
BtsCI	GGATG	4 cut(s)	722, 757, 935, 1087
BtsI	GCAGTG	1 cut(s)	285
BtsIMutI	CAGTG	3 cut(s)	285, 286, 1396
Cac8I	GCNNGC	3 cut(s)	1258, 1441, 1445
CaiI	CAGNNNCTG	1 cut(s)	1436
CciI	TCATGA	1 cut(s)	558
CfoI	GCGC	1 cut(s)	1449
Cfr10I	RCCGGY	1 cut(s)	530
Cfr13I	GGNCC	2 cut(s)	187, 1275
CseI	GACGC	1 cut(s)	1316
Csp6I	GTAC	6 cut(s)	53, 77, 98, 293, 404, 734
CspAI	ACCGGT	1 cut(s)	530
CviAII	CATG	4 cut(s)	129, 559, 851, 1444
CviQI	GTAC	6 cut(s)	53, 77, 98, 293, 404, 734
DdeI	CTNAG	4 cut(s)	182, 705, 939, 1371
DpnI	GATC	6 cut(s)	27, 227, 369, 657, 710, 781
DpnII	GATC	6 cut(s)	25, 225, 367, 655, 708, 779
Eam1104I	CTCTTC	1 cut(s)	669
EarI	CTCTTC	1 cut(s)	669
Eco24I	GRGCYC	1 cut(s)	1059
Eco47I	GGWCC	1 cut(s)	1275
Eco88I	CYCGRG	1 cut(s)	810
EcoO109I	RGGNCCY	2 cut(s)	187, 1275
EcoRII	CCWGG	6 cut(s)	205, 388, 712, 746, 757, 953
EcoT22I	ATGCAT	1 cut(s)	1128
EcoT38I	GRGCYC	1 cut(s)	1059
FaeI	CATG	4 cut(s)	132, 562, 854, 1447
FaqI	GGGAC	1 cut(s)	740
FatI	CATG	4 cut(s)	128, 558, 850, 1443
FauNDI	CATATG	2 cut(s)	22, 31
FblI	GTMKAC	2 cut(s)	1039, 1367
FokI	GGATG	4 cut(s)	729, 764, 942, 1074
FriOI	GRGCYC	1 cut(s)	1059
FspBI	CTAG	4 cut(s)	155, 435, 668, 1385
GlaI	GCGC	1 cut(s)	1448
GsaI	CCCAGC	1 cut(s)	90
GsuI	CTGGAG	3 cut(s)	33, 411, 1053
HaeIII	GGCC	1 cut(s)	189
HapII	CCGG	1 cut(s)	531
HgaI	GACGC	1 cut(s)	1316
HhaI	GCGC	1 cut(s)	1449
Hin1II	CATG	4 cut(s)	132, 562, 854, 1447
Hin6I	GCGC	1 cut(s)	1447
HinP1I	GCGC	1 cut(s)	1447
HincII	GTYRAC	1 cut(s)	1368
HindII	GTYRAC	1 cut(s)	1368
HindIII	AAGCTT	1 cut(s)	605
HinfI	GANTC	5 cut(s)	762, 935, 977, 1004, 1369
HpaII	CCGG	1 cut(s)	531
HphI	GGTGA	3 cut(s)	548, 1327, 1351
Hpy166II	GTNNAC	7 cut(s)	277, 295, 553, 883, 1040, 1239, 1368
Hpy188I	TCNGA	3 cut(s)	108, 940, 1035
Hpy188III	TCNNGA	7 cut(s)	559, 653, 1008, 1070, 1091, 1229, 1373
Hpy8I	GTNNAC	7 cut(s)	277, 295, 553, 883, 1040, 1239, 1368
HpyAV	CCTTC	6 cut(s)	75, 669, 817, 1184, 1342, 1366
HpyCH4III	ACNGT	3 cut(s)	250, 281, 889
HpyCH4IV	ACGT	1 cut(s)	1042
HpyCH4V	TGCA	8 cut(s)	132, 323, 518, 553, 593, 835, 1126, 1439
HpyF10VI	GCNNNNNNNGC	8 cut(s)	195, 815, 824, 1394, 1403, 1406, 1433, 1436
HpyF3I	CTNAG	4 cut(s)	182, 705, 939, 1371
HpySE526I	ACGT	1 cut(s)	1042
Hsp92II	CATG	4 cut(s)	132, 562, 854, 1447
HspAI	GCGC	1 cut(s)	1447
Kzo9I	GATC	6 cut(s)	25, 225, 367, 655, 708, 779
LmnI	GCTCC	5 cut(s)	10, 52, 76, 1394, 1414
MaeI	CTAG	4 cut(s)	155, 435, 668, 1385
MaeII	ACGT	1 cut(s)	1042
MaeIII	GTNAC	3 cut(s)	353, 906, 1201
MalI	GATC	6 cut(s)	27, 227, 369, 657, 710, 781
MbiI	CCGCTC	1 cut(s)	815
MboI	GATC	6 cut(s)	25, 225, 367, 655, 708, 779
MboII	GAAGA	6 cut(s)	188, 235, 281, 656, 1150, 1311
MflI	RGATCY	3 cut(s)	225, 708, 779
MhlI	GDGCHC	3 cut(s)	555, 1059, 1294
MluCI	AATT	5 cut(s)	301, 562, 830, 993, 1250
MlyI	GAGTC	3 cut(s)	771, 986, 1363
Mph1103I	ATGCAT	1 cut(s)	1128
MroXI	GAANNNNTTC	1 cut(s)	1380
MseI	TTAA	6 cut(s)	168, 213, 416, 612, 767, 900
MspA1I	CMGCKG	2 cut(s)	326, 1433
MspI	CCGG	1 cut(s)	531
MspR9I	CCNGG	6 cut(s)	207, 390, 714, 748, 759, 955
Mva1269I	GAATGC	1 cut(s)	518
MvaI	CCWGG	6 cut(s)	207, 390, 714, 748, 759, 955
MwoI	GCNNNNNNNGC	8 cut(s)	195, 815, 824, 1394, 1403, 1406, 1433, 1436
NdeI	CATATG	2 cut(s)	22, 31
NdeII	GATC	6 cut(s)	25, 225, 367, 655, 708, 779
NlaIII	CATG	4 cut(s)	132, 562, 854, 1447
NlaIV	GGNNCC	2 cut(s)	6, 188
NmuCI	GTSAC	1 cut(s)	1201
NsiI	ATGCAT	1 cut(s)	1128
NspI	RCATGY	1 cut(s)	1447
PaeI	GCATGC	1 cut(s)	1447
PagI	TCATGA	1 cut(s)	558
PasI	CCCWGGG	1 cut(s)	954
PctI	GAATGC	1 cut(s)	518
PdmI	GAANNNNTTC	1 cut(s)	1380
PfeI	GAWTC	2 cut(s)	935, 1004
PinAI	ACCGGT	1 cut(s)	530
PleI	GAGTC	3 cut(s)	770, 985, 1363
PpsI	GAGTC	3 cut(s)	770, 985, 1363
PpuMI	RGGWCCY	1 cut(s)	1275
PshBI	ATTAAT	1 cut(s)	612
Psp5II	RGGWCCY	1 cut(s)	1275
Psp6I	CCWGG	6 cut(s)	205, 388, 712, 746, 757, 953
PspFI	CCCAGC	1 cut(s)	86
PspGI	CCWGG	6 cut(s)	205, 388, 712, 746, 757, 953
PspN4I	GGNNCC	2 cut(s)	6, 188
PspPI	GGNCC	2 cut(s)	187, 1275
PspPPI	RGGWCCY	1 cut(s)	1275
PstNI	CAGNNNCTG	1 cut(s)	1436
PsuI	RGATCY	3 cut(s)	225, 708, 779
PvuII	CAGCTG	2 cut(s)	326, 1433
RsaI	GTAC	6 cut(s)	54, 78, 99, 294, 405, 735
RsaNI	GTAC	6 cut(s)	53, 77, 98, 293, 404, 734
SalI	GTCGAC	1 cut(s)	1366
SaqAI	TTAA	6 cut(s)	168, 213, 416, 612, 767, 900
Sau3AI	GATC	6 cut(s)	25, 225, 367, 655, 708, 779
Sau96I	GGNCC	2 cut(s)	187, 1275
SchI	GAGTC	3 cut(s)	771, 986, 1363
ScrFI	CCNGG	6 cut(s)	207, 390, 714, 748, 759, 955
SduI	GDGCHC	3 cut(s)	555, 1059, 1294
SinI	GGWCC	1 cut(s)	1275
SphI	GCATGC	1 cut(s)	1447
Sse9I	AATT	5 cut(s)	301, 562, 830, 993, 1250
SsiI	CCGC	2 cut(s)	815, 1428
SspMI	CTAG	4 cut(s)	155, 435, 668, 1385
StyD4I	CCNGG	6 cut(s)	205, 388, 712, 746, 757, 953
TaaI	ACNGT	3 cut(s)	250, 281, 889
TaiI	ACGT	1 cut(s)	1045
TaqI	TCGA	1 cut(s)	1367
TasI	AATT	5 cut(s)	301, 562, 830, 993, 1250
TatI	WGTACW	2 cut(s)	292, 733
TauI	GCSGC	2 cut(s)	818, 1430
TfiI	GAWTC	2 cut(s)	935, 1004
Tru1I	TTAA	6 cut(s)	168, 213, 416, 612, 767, 900
Tru9I	TTAA	6 cut(s)	168, 213, 416, 612, 767, 900
TscAI	CASTG	3 cut(s)	286, 292, 1396
TseFI	GTSAC	1 cut(s)	1201
Tsp45I	GTSAC	1 cut(s)	1201
TspDTI	ATGAA	5 cut(s)	236, 247, 547, 575, 1122
TspGWI	ACGGA	1 cut(s)	577
TspRI	CASTG	3 cut(s)	286, 292, 1396
VneI	GTGCAC	1 cut(s)	551
VpaK11BI	GGWCC	1 cut(s)	1275
VspI	ATTAAT	1 cut(s)	612
XceI	RCATGY	1 cut(s)	1447
XmiI	GTMKAC	2 cut(s)	1039, 1367
XmnI	GAANNNNTTC	1 cut(s)	1380
XspI	CTAG	4 cut(s)	155, 435, 668, 1385
Zsp2I	ATGCAT	1 cut(s)	1128

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.