Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

contig_6

Orthogroup ID

OG0001609

Physical Location & Seq

Genomic Coordinates Forward (+)

36963 .. 41584

Gene Structure 4622 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_c6g00050.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2415 bp

ATGCCTGAAGATGCAATATTAGAAGACGCTGCAGATCGTACAGAAAGCCATGATTGTCAAGAAGAAGAAGCTCATCATCGACAACTCAAAACCAAGAAACTTTCATGGCAAAAGTTACGTAGATTTGACTCCTTGGACATCGAGTCACGCAGTGTCAACGGTCACCACGGCCATAGCTCCACGGATGCAAGTTGGTCTGTAATCTTGCAACTAGCATTTCAGAGCATTGGAATAGTCTATGGAGACATTGGTACATCACCTCTATATGTGTATGCGAGTACCTTCAGCAAAGGCATCAAGGACACAGATGATATTTTGGGTGTTCTTTCTTTGATCATATACACCATCACCTTAATCCCCTTGATCAAATACGTTTTCATCGTCCTCCAAGCTAACGATAATGGCGACGGAGGGACGTTTGCACTGTACTCTCTGTTGTGTCGATATGCCAAGGTTAGTTTAACTCCGAGTCAACAAGCTGAAGACCGCGATGTTTCAAATTTCCAACTTGAGTTGCCTAGCAAACGTCTAAGGAGGGCATCAAAGCTTAAATCAAAACTAGAGAACAGCAAGTTTGCCAAATACTTTTTACTCTTTGCCACCATGCTTGGTACTTCTATGGTTATTGGTGATGGTGTCCTCACTCCTTGCATCTCAGTTCTATCGGCTGTTGGAGGGATAAAACAAGCTACATCTGCTATGACAGACGATATGATTGTATGGATATCAGTATGCATCTTGGTTTGCCTATTCATGGCTCAAAGATTTGGAACTGACAAAGTTGGCTACAGTTTTGCACCAATTATTTGTGTTTGGTTTACCCTCATTGGTGGCATTGGCGTGTACAATTTCTTCAAATACGATCCAACAGTTTTAAAAGCTCTAAATCCACAATACATAGTCGATTACTTCAAGAGAAACAAAGGAGATGCTTGGATTTCCCTTGGTGGCATTGTCCTTGCCATTACAGGAACTGAAGCTCTGTTTGCCGATGTTGGCCACTTCACAGTTCGATCCATTCAGATTAGTATGTGCTCAGTGACTTACCCAGCTCTCCTGCTCGCATACACAGGACAAGCCTCTTTTCTTCGCGAGCACAAGAGCCTAGTTTCAGACACGTTCTTTGAGTCCATACCAAAACCTTTGTATTGGCCTATGTTTGGAGTTGCTGTAATGGCATCAATCATAGCCAGTCAAGCTATGATTTCAGGTACGTTCTCAATCATCCAACAATCCCTTTCACTAGGGTGTTTCCCTCGTGTGAAAATTGTGCACACATCGACCAAGTATGAAGGACAAGTTTACATTCCGGAGGTCAACTACCTTCTGATGTTGGCTTGTGTTGGAGTGACCTTGGTTTTCAGAAGCACCACAAAGATTGGAAATGCATACGGCATAGCAGTTGTATTTGTAATGACGCTTACATCGGCCTTCCTGGTGTTAATCATGATTATGATTTGGAAAACCCATATACTTCTCATCATTTCATATGTTCTTGTCATCGGAAGCGTGGAGCTCATGTACTTAAGTTCGGTGCTCTACAAGTTTGATCAAGGTGGATATCTTCCTCTCGGATTTGCGCTTTTATTGATGCTCATAATGTTTGTGTGGAACGATGTTCATCGAAGAAAGTACTACTACGAGCTCGATCACAAAATTTCCCCAGAAAAGCTCAGGCAACTTGCTGTGGACAAAAACTTCTGCCGCATGCCTGGCCTCGCCATGTTCTACTCAGAACTCGTTCAAGGAATTCCTCCAATCTTCAATCATTACGCGGAGAATGTGCCTGCTTTGCACTCAGTCTTGGTTTTTGTCTCCATCAAATCGTTACCAATAAGCAAGGTTCCTATGGAGGAGCGTTTTCTTTTCCGCAGAGTGGAGCCTAGGGAGCTTAACGTGTTCCGATGTGTCGCTAGATATGGGTACACAGATGTTCGAAATGAGAATGAACCCTTTGAAGGATTGCTGGTAGAAAAATTGAAAGACTTCATAAGGGATGACTTTTGGCAATCTCAAACAACTATGCACAAAACAAATGGAGAGAAATTGAAAAAAGAAGTTTCTGCTGAGTTATTAGGTGATGATCATGAGCTGGATAATGGAAATGGTCATCAAGAGAATGAGAGCGAGAACGAGGATGTAAAGCAAGTATTGGATGATGATGAAGATCAAAAGAAACAACAAGATGATGTGTTGGGGACAGAGATTGACGCAATTGACAAAGCGTGGCGCCGGGGAGTGGTGCACCTAATTGGCGAGAATGAAGTGACGTCTGCTAAAGGAGCTGGGTTAGCAAAAAGGATCTTGATTGATTATGCTTACAATTTCTTGACGAGAAATCTGAGACAAAGTGAAAAAGTGTTTGATATTCCTCACAAGCGCATGTTAAAAGTGGGCATGACTTACGAACTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

805

Amino Acids

90.73

Weight (kDa)

6.37

Isoelectric Point (pI)

34.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	9 - 348	1.1e-121	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	362 - 595	5.5e-26	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001609)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30541 FvH4_6g30550 FvH4_c6g00050 FvH4_c6g00050 FvH4_c6g00050
malus_domestica	MD03G1283500.v1.1 MD03G1283600.v1.1 MD10G1230200.v1.1 MD10G1230300.v1.1 MD11G1302600.v1.1 MD11G1302900.v1.1 MD11G1303100.v1.1
prunus_persica	Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1
pyrus_communis	pycom03g22450 pycom03g22460
rosa_chinensis	RchiOBHm_Chr2g0136981 RchiOBHm_Chr5g0020481 RchiOBHm_Chr6g0293251
rosa_laevigata	RLG00000011964 RLG00000033905
rosa_multiflora	Rmu_co8314359.1_g000001 Rmu_sc0001374.1_g000063 Rmu_sc0001374.1_g000064 Rmu_sc0021490.1_g000001 Rmu_ssc0000162.1_g000030
rosa_roxburghii	Rroxscaffold_2G00107800 Rroxscaffold_2G00107830 Rroxscaffold_7G00174310
rosa_rugosa	Rorug02G0336400 Rorug06G0233800 Rorug06G0233800 Rorug06G0233800
rosa_samantha	Rh2AG385800 Rh2BG392700 Rh2CG373000 Rh2DG047100 Rh2DG408800 Rh2DG408900 Rh3DG241600 Rh3DG283500 Rh6AG346700 Rh6BG354100 Rh6CG360600 Rh6DG347100
rosa_wichuraiana	Rw2G003360 Rw2G031570 Rw6G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2273
AccB1I	GGYRCC	1 cut(s)	2229
AccII	CGCG	3 cut(s)	489, 1092, 1775
AccIII	TCCGGA	1 cut(s)	1309
AciI	CCGC	4 cut(s)	487, 1705, 1775, 1870
AclWI	GGATC	3 cut(s)	857, 1008, 2309
AcoI	YGGCCR	2 cut(s)	169, 997
AcsI	RAATTY	3 cut(s)	499, 1656, 1749
AcuI	CTGAAG	4 cut(s)	27, 268, 501, 996
AcyI	GRCGYC	2 cut(s)	2230, 2270
AdeI	CACNNNGTG	1 cut(s)	152
AfiI	CCNNNNNNNGG	5 cut(s)	754, 1160, 1876, 1958, 2239
AflII	CTTAAG	1 cut(s)	1525
AflIII	ACRYGT	2 cut(s)	1116, 1896
AgsI	TTSAA	8 cut(s)	498, 856, 913, 1745, 1765, 1958, 1981, 2050
AhdI	GACNNNNNGTC	1 cut(s)	142
AjnI	CCWGG	2 cut(s)	1434, 1711
AleI	CACNNNNGTG	1 cut(s)	1246
Alw21I	GWGCWC	7 cut(s)	1037, 1098, 1275, 1518, 1539, 1647, 2247
Alw26I	GTCTC	3 cut(s)	237, 1819, 2338
Alw44I	GTGCAC	2 cut(s)	1271, 2243
AlwI	GGATC	3 cut(s)	857, 1008, 2309
AlwNI	CAGNNNCTG	1 cut(s)	974
Aor13HI	TCCGGA	1 cut(s)	1309
AoxI	GGCC	5 cut(s)	169, 997, 1151, 1428, 1714
ApaLI	GTGCAC	2 cut(s)	1271, 2243
ApeKI	GCWGC	1 cut(s)	29
ApoI	RAATTY	3 cut(s)	499, 1656, 1749
ArsI	GACNNNNNNTTYG	2 cut(s)	1106, 1138
Asp700I	GAANNNNTTC	2 cut(s)	1740, 1985
AspA2I	CCTAGG	1 cut(s)	1883
AspLEI	GCGC	3 cut(s)	1582, 2232, 2382
AsuC2I	CCSGG	1 cut(s)	2234
AsuHPI	GGTGA	5 cut(s)	155, 249, 340, 641, 2090
AsuII	TTCGAA	1 cut(s)	1936
AvrII	CCTAGG	1 cut(s)	1883
BaeGI	GKGCMC	2 cut(s)	1275, 2247
BalI	TGGCCA	1 cut(s)	999
BanI	GGYRCC	1 cut(s)	2229
BanII	GRGCYC	2 cut(s)	1518, 1647
BarI	GAAGNNNNNNTAC	2 cut(s)	836, 868
BauI	CACGAG	1 cut(s)	1257
BbsI	GAAGAC	2 cut(s)	30, 489
Bbv12I	GWGCWC	7 cut(s)	1037, 1098, 1275, 1518, 1539, 1647, 2247
BbvI	GCAGC	1 cut(s)	16
BccI	CCATC	3 cut(s)	353, 626, 1826
BceAI	ACGGC	2 cut(s)	184, 1408
BciT130I	CCWGG	2 cut(s)	1436, 1713
BclI	TGATCA	4 cut(s)	333, 363, 1549, 2083
BcnI	CCSGG	1 cut(s)	2234
BcoDI	GTCTC	3 cut(s)	237, 1819, 2338
BfaI	CTAG	7 cut(s)	212, 519, 560, 1106, 1244, 1884, 1914
BfmI	CTRYAG	3 cut(s)	30, 787, 2411
BfoI	RGCGCY	1 cut(s)	2233
BfrI	CTTAAG	1 cut(s)	1525
BisI	GCNGC	2 cut(s)	30, 1705
BlnI	CCTAGG	1 cut(s)	1883
BlsI	GCNGC	2 cut(s)	31, 1706
BmcAI	AGTACT	1 cut(s)	1634
Bme1390I	CCNGG	3 cut(s)	1436, 1713, 2234
BmeRI	GACNNNNNGTC	1 cut(s)	142
BmiI	GGNNCC	3 cut(s)	1845, 1881, 2231
BmrFI	CCNGG	3 cut(s)	1436, 1713, 2234
BmsI	GCATC	8 cut(s)	175, 303, 548, 660, 744, 919, 1187, 1581
BpiI	GAAGAC	2 cut(s)	30, 489
Bpu10I	CCTNAGC	1 cut(s)	1673
Bpu14I	TTCGAA	1 cut(s)	1936
BpuEI	CTTGAG	1 cut(s)	530
BpuMI	CCSGG	1 cut(s)	2234
BsaAI	YACGTR	1 cut(s)	119
BsaBI	GATNNNNATC	2 cut(s)	1620, 2166
BsaHI	GRCGYC	2 cut(s)	2230, 2270
BsaJI	CCNNGG	8 cut(s)	132, 166, 180, 450, 943, 1353, 1883, 2233
BsaWI	WCCGGW	1 cut(s)	1309
BsaXI	ACNNNNNCTCC	4 cut(s)	1155, 1185, 1505, 1535
Bsc4I	CCNNNNNNNGG	5 cut(s)	754, 1160, 1876, 1958, 2239
Bse1I	ACTGG	1 cut(s)	1191
Bse8I	GATNNNNATC	2 cut(s)	1620, 2166
BseAI	TCCGGA	1 cut(s)	1309
BseBI	CCWGG	2 cut(s)	1436, 1713
BseDI	CCNNGG	8 cut(s)	132, 166, 180, 450, 943, 1353, 1883, 2233
BseGI	GGATG	5 cut(s)	190, 1224, 2002, 2143, 2161
BseJI	GATNNNNATC	2 cut(s)	1620, 2166
BseLI	CCNNNNNNNGG	5 cut(s)	754, 1160, 1876, 1958, 2239
BseMII	CTCAG	7 cut(s)	669, 1050, 1687, 1746, 1812, 2058, 2333
BseNI	ACTGG	1 cut(s)	1191
BseRI	GAGGAG	1 cut(s)	1868
BseSI	GKGCMC	2 cut(s)	1275, 2247
BseXI	GCAGC	1 cut(s)	16
BseYI	CCCAGC	2 cut(s)	1048, 2285
Bsh1236I	CGCG	3 cut(s)	489, 1092, 1775
BshFI	GGCC	5 cut(s)	171, 999, 1153, 1430, 1716
BshNI	GGYRCC	1 cut(s)	2229
BsiHKAI	GWGCWC	7 cut(s)	1037, 1098, 1275, 1518, 1539, 1647, 2247
BsiSI	CCGG	2 cut(s)	1310, 2233
BslFI	GGGAC	2 cut(s)	427, 2212
BslI	CCNNNNNNNGG	5 cut(s)	754, 1160, 1876, 1958, 2239
BsmAI	GTCTC	3 cut(s)	237, 1819, 2338
BsmFI	GGGAC	2 cut(s)	427, 2212
BsnI	GGCC	5 cut(s)	171, 999, 1153, 1430, 1716
Bsp119I	TTCGAA	1 cut(s)	1936
Bsp1286I	GDGCHC	7 cut(s)	1037, 1098, 1275, 1518, 1539, 1647, 2247
Bsp13I	TCCGGA	1 cut(s)	1309
Bsp1407I	TGTACA	1 cut(s)	843
Bsp68I	TCGCGA	1 cut(s)	1092
BspACI	CCGC	4 cut(s)	487, 1705, 1775, 1870
BspANI	GGCC	5 cut(s)	171, 999, 1153, 1430, 1716
BspCNI	CTCAG	7 cut(s)	668, 1049, 1686, 1745, 1811, 2059, 2334
BspEI	TCCGGA	1 cut(s)	1309
BspFNI	CGCG	3 cut(s)	489, 1092, 1775
BspHI	TCATGA	2 cut(s)	1446, 2086
BspLI	GGNNCC	3 cut(s)	1845, 1881, 2231
BspMAI	CTGCAG	1 cut(s)	34
BspPI	GGATC	3 cut(s)	857, 1008, 2309
BspT104I	TTCGAA	1 cut(s)	1936
BspT107I	GGYRCC	1 cut(s)	2229
BspTI	CTTAAG	1 cut(s)	1525
BsrGI	TGTACA	1 cut(s)	843
BsrI	ACTGG	1 cut(s)	1191
BssECI	CCNNGG	8 cut(s)	132, 166, 180, 450, 943, 1353, 1883, 2233
BssNI	GRCGYC	2 cut(s)	2230, 2270
BssSI	CACGAG	1 cut(s)	1257
BssT1I	CCWWGG	5 cut(s)	132, 450, 943, 1353, 1883
Bst2BI	CACGAG	1 cut(s)	1257
Bst2UI	CCWGG	2 cut(s)	1436, 1713
Bst4CI	ACNGT	5 cut(s)	161, 426, 791, 871, 1009
BstACI	GRCGYC	2 cut(s)	2230, 2270
BstAFI	CTTAAG	1 cut(s)	1525
BstAUI	TGTACA	1 cut(s)	843
BstBAI	YACGTR	1 cut(s)	119
BstBI	TTCGAA	1 cut(s)	1936
BstC8I	GCNNGC	4 cut(s)	1062, 1094, 1709, 1788
BstDEI	CTNAG	8 cut(s)	530, 655, 1036, 1673, 1732, 1798, 2067, 2342
BstDSI	CCRYGG	2 cut(s)	166, 180
BstEII	GGTNACC	1 cut(s)	161
BstF5I	GGATG	5 cut(s)	190, 1224, 2002, 2143, 2161
BstFNI	CGCG	3 cut(s)	489, 1092, 1775
BstH2I	RGCGCY	1 cut(s)	2233
BstHHI	GCGC	3 cut(s)	1582, 2232, 2382
BstMAI	GTCTC	3 cut(s)	237, 1819, 2338
BstMWI	GCNNNNNNNGC	9 cut(s)	695, 986, 1175, 1196, 1713, 1792, 1888, 2282, 2291
BstNI	CCWGG	2 cut(s)	1436, 1713
BstNSI	RCATGY	2 cut(s)	1711, 2386
BstPI	GGTNACC	1 cut(s)	161
BstSCI	CCNGG	3 cut(s)	1434, 1711, 2232
BstSFI	CTRYAG	3 cut(s)	30, 787, 2411
BstSLI	GKGCMC	2 cut(s)	1275, 2247
BstSNI	TACGTA	1 cut(s)	119
BstUI	CGCG	3 cut(s)	489, 1092, 1775
BstV1I	GCAGC	1 cut(s)	16
BstV2I	GAAGAC	2 cut(s)	30, 489
BstX2I	RGATCY	1 cut(s)	2301
BstYI	RGATCY	1 cut(s)	2301
BsuRI	GGCC	5 cut(s)	171, 999, 1153, 1430, 1716
BtgI	CCRYGG	2 cut(s)	166, 180
BtgZI	GCGATG	1 cut(s)	504
BtsCI	GGATG	5 cut(s)	190, 1224, 2002, 2143, 2161
BtsI	GCAGTG	1 cut(s)	157
BtsIMutI	CAGTG	3 cut(s)	157, 422, 1044
BtuMI	TCGCGA	1 cut(s)	1092
Cac8I	GCNNGC	4 cut(s)	1062, 1094, 1709, 1788
CaiI	CAGNNNCTG	1 cut(s)	974
CciI	TCATGA	2 cut(s)	1446, 2086
CfoI	GCGC	3 cut(s)	1582, 2232, 2382
CseI	GACGC	3 cut(s)	35, 1426, 2219
CspCI	CAANNNNNGTGG	4 cut(s)	589, 624, 1360, 1395
DdeI	CTNAG	8 cut(s)	530, 655, 1036, 1673, 1732, 1798, 2067, 2342
DinI	GGCGCC	1 cut(s)	2231
DraI	TTTAAA	1 cut(s)	876
DraIII	CACNNNGTG	1 cut(s)	152
DriI	GACNNNNNGTC	1 cut(s)	142
EaeI	YGGCCR	2 cut(s)	169, 997
Eam1105I	GACNNNNNGTC	1 cut(s)	142
Ecl136II	GAGCTC	2 cut(s)	1516, 1645
Eco105I	TACGTA	1 cut(s)	119
Eco130I	CCWWGG	5 cut(s)	132, 450, 943, 1353, 1883
Eco24I	GRGCYC	2 cut(s)	1518, 1647
Eco32I	GATATC	2 cut(s)	726, 1562
Eco53kI	GAGCTC	2 cut(s)	1516, 1645
Eco57I	CTGAAG	4 cut(s)	27, 268, 501, 996
Eco91I	GGTNACC	1 cut(s)	161
EcoICRI	GAGCTC	2 cut(s)	1516, 1645
EcoO65I	GGTNACC	1 cut(s)	161
EcoRI	GAATTC	1 cut(s)	1749
EcoRII	CCWGG	2 cut(s)	1434, 1711
EcoRV	GATATC	2 cut(s)	726, 1562
EcoT14I	CCWWGG	5 cut(s)	132, 450, 943, 1353, 1883
EcoT22I	ATGCAT	2 cut(s)	737, 1390
EcoT38I	GRGCYC	2 cut(s)	1518, 1647
EgeI	GGCGCC	1 cut(s)	2231
EheI	GGCGCC	1 cut(s)	2231
ErhI	CCWWGG	5 cut(s)	132, 450, 943, 1353, 1883
FaqI	GGGAC	2 cut(s)	427, 2212
FauNDI	CATATG	1 cut(s)	1489
FbaI	TGATCA	4 cut(s)	333, 363, 1549, 2083
Fnu4HI	GCNGC	2 cut(s)	30, 1705
FokI	GGATG	5 cut(s)	197, 1211, 2009, 2150, 2168
FriOI	GRGCYC	2 cut(s)	1518, 1647
Fsp4HI	GCNGC	2 cut(s)	30, 1705
FspBI	CTAG	7 cut(s)	212, 519, 560, 1106, 1244, 1884, 1914
GlaI	GCGC	3 cut(s)	1581, 2231, 2381
GluI	GCNGC	2 cut(s)	30, 1705
GsaI	CCCAGC	2 cut(s)	1052, 2289
HaeII	RGCGCY	1 cut(s)	2233
HaeIII	GGCC	5 cut(s)	171, 999, 1153, 1430, 1716
HapII	CCGG	2 cut(s)	1310, 2233
HgaI	GACGC	3 cut(s)	35, 1426, 2219
HhaI	GCGC	3 cut(s)	1582, 2232, 2382
Hin1I	GRCGYC	2 cut(s)	2230, 2270
Hin6I	GCGC	3 cut(s)	1580, 2230, 2380
HinP1I	GCGC	3 cut(s)	1580, 2230, 2380
HincII	GTYRAC	3 cut(s)	157, 473, 1318
HindII	GTYRAC	3 cut(s)	157, 473, 1318
HindIII	AAGCTT	1 cut(s)	545
HinfI	GANTC	4 cut(s)	128, 143, 469, 1127
HpaII	CCGG	2 cut(s)	1310, 2233
HphI	GGTGA	5 cut(s)	155, 249, 340, 641, 2090
Hpy188III	TCNNGA	9 cut(s)	59, 913, 1091, 1310, 1447, 2087, 2114, 2305, 2329
Hpy99I	CGWCG	1 cut(s)	410
HpyAV	CCTTC	5 cut(s)	292, 1286, 1334, 1441, 1952
HpyCH4III	ACNGT	5 cut(s)	161, 426, 791, 871, 1009
HpyCH4IV	ACGT	8 cut(s)	118, 372, 416, 526, 1118, 1214, 1896, 2270
HpyF10VI	GCNNNNNNNGC	9 cut(s)	695, 986, 1175, 1196, 1713, 1792, 1888, 2282, 2291
HpyF3I	CTNAG	8 cut(s)	530, 655, 1036, 1673, 1732, 1798, 2067, 2342
HpySE526I	ACGT	8 cut(s)	118, 372, 416, 526, 1118, 1214, 1896, 2270
Hsp92I	GRCGYC	2 cut(s)	2230, 2270
HspAI	GCGC	3 cut(s)	1580, 2230, 2380
KasI	GGCGCC	1 cut(s)	2229
Kpn2I	TCCGGA	1 cut(s)	1309
Ksp22I	TGATCA	4 cut(s)	333, 363, 1549, 2083
LmnI	GCTCC	6 cut(s)	182, 1513, 1855, 1879, 1888, 2282
Lsp1109I	GCAGC	1 cut(s)	16
LweI	GCATC	8 cut(s)	175, 303, 548, 660, 744, 919, 1187, 1581
MaeI	CTAG	7 cut(s)	212, 519, 560, 1106, 1244, 1884, 1914
MaeII	ACGT	8 cut(s)	118, 372, 416, 526, 1118, 1214, 1896, 2270
MaeIII	GTNAC	7 cut(s)	114, 144, 161, 1039, 1348, 1827, 2266
MfeI	CAATTG	1 cut(s)	2214
MflI	RGATCY	1 cut(s)	2301
MhlI	GDGCHC	7 cut(s)	1037, 1098, 1275, 1518, 1539, 1647, 2247
MlsI	TGGCCA	1 cut(s)	999
MluNI	TGGCCA	1 cut(s)	999
Mly113I	GGCGCC	1 cut(s)	2230
MlyI	GAGTC	4 cut(s)	122, 152, 478, 1136
MmeI	TCCRAC	5 cut(s)	529, 652, 890, 1252, 1324
Mox20I	TGGCCA	1 cut(s)	999
Mph1103I	ATGCAT	2 cut(s)	737, 1390
MroI	TCCGGA	1 cut(s)	1309
MroXI	GAANNNNTTC	2 cut(s)	1740, 1985
MscI	TGGCCA	1 cut(s)	999
MseI	TTAA	8 cut(s)	353, 461, 549, 875, 1442, 1526, 1893, 2387
MslI	CAYNNNNRTG	3 cut(s)	839, 1246, 1451
Msp20I	TGGCCA	1 cut(s)	999
MspCI	CTTAAG	1 cut(s)	1525
MspI	CCGG	2 cut(s)	1310, 2233
MspR9I	CCNGG	3 cut(s)	1436, 1713, 2234
MunI	CAATTG	1 cut(s)	2214
MvaI	CCWGG	2 cut(s)	1436, 1713
MvnI	CGCG	3 cut(s)	489, 1092, 1775
MwoI	GCNNNNNNNGC	9 cut(s)	695, 986, 1175, 1196, 1713, 1792, 1888, 2282, 2291
NarI	GGCGCC	1 cut(s)	2230
NciI	CCSGG	1 cut(s)	2234
NdeI	CATATG	1 cut(s)	1489
NlaIV	GGNNCC	3 cut(s)	1845, 1881, 2231
NmuCI	GTSAC	5 cut(s)	144, 161, 1039, 1348, 2266
NruI	TCGCGA	1 cut(s)	1092
NsiI	ATGCAT	2 cut(s)	737, 1390
NspI	RCATGY	2 cut(s)	1711, 2386
NspV	TTCGAA	1 cut(s)	1936
OliI	CACNNNNGTG	1 cut(s)	1246
PaeI	GCATGC	1 cut(s)	1711
PagI	TCATGA	2 cut(s)	1446, 2086
PcsI	WCGNNNNNNNCGW	2 cut(s)	378, 402
PdmI	GAANNNNTTC	2 cut(s)	1740, 1985
PkrI	GCNGC	2 cut(s)	31, 1706
PleI	GAGTC	4 cut(s)	122, 151, 477, 1135
PluTI	GGCGCC	1 cut(s)	2233
PpsI	GAGTC	4 cut(s)	122, 151, 477, 1135
Ppu21I	YACGTR	1 cut(s)	119
Psp124BI	GAGCTC	2 cut(s)	1518, 1647
Psp6I	CCWGG	2 cut(s)	1434, 1711
PspEI	GGTNACC	1 cut(s)	161
PspFI	CCCAGC	2 cut(s)	1048, 2285
PspGI	CCWGG	2 cut(s)	1434, 1711
PspN4I	GGNNCC	3 cut(s)	1845, 1881, 2231
PstI	CTGCAG	1 cut(s)	34
PstNI	CAGNNNCTG	1 cut(s)	974
PsuI	RGATCY	1 cut(s)	2301
RruI	TCGCGA	1 cut(s)	1092
RseI	CAYNNNNRTG	3 cut(s)	839, 1246, 1451
SacI	GAGCTC	2 cut(s)	1518, 1647
SaqAI	TTAA	8 cut(s)	353, 461, 549, 875, 1442, 1526, 1893, 2387
SatI	GCNGC	2 cut(s)	30, 1705
ScaI	AGTACT	1 cut(s)	1634
SchI	GAGTC	4 cut(s)	122, 152, 478, 1136
ScrFI	CCNGG	3 cut(s)	1436, 1713, 2234
SduI	GDGCHC	7 cut(s)	1037, 1098, 1275, 1518, 1539, 1647, 2247
SfaNI	GCATC	8 cut(s)	175, 303, 548, 660, 744, 919, 1187, 1581
SfcI	CTRYAG	3 cut(s)	30, 787, 2411
SfoI	GGCGCC	1 cut(s)	2231
SfuI	TTCGAA	1 cut(s)	1936
SmiMI	CAYNNNNRTG	3 cut(s)	839, 1246, 1451
SmlI	CTYRAG	2 cut(s)	509, 1525
SmoI	CTYRAG	2 cut(s)	509, 1525
SnaBI	TACGTA	1 cut(s)	119
SphI	GCATGC	1 cut(s)	1711
SsiI	CCGC	4 cut(s)	487, 1705, 1775, 1870
SspDI	GGCGCC	1 cut(s)	2229
SspI	AATATT	1 cut(s)	18
SspMI	CTAG	7 cut(s)	212, 519, 560, 1106, 1244, 1884, 1914
SstI	GAGCTC	2 cut(s)	1518, 1647
StyD4I	CCNGG	3 cut(s)	1434, 1711, 2232
StyI	CCWWGG	5 cut(s)	132, 450, 943, 1353, 1883
TaaI	ACNGT	5 cut(s)	161, 426, 791, 871, 1009
TaiI	ACGT	8 cut(s)	121, 375, 419, 529, 1121, 1217, 1899, 2273
TaqI	TCGA	9 cut(s)	79, 141, 442, 903, 1012, 1280, 1624, 1647, 1936
TatI	WGTACW	4 cut(s)	426, 843, 1521, 1632
TauI	GCSGC	1 cut(s)	1707
Tru1I	TTAA	8 cut(s)	353, 461, 549, 875, 1442, 1526, 1893, 2387
Tru9I	TTAA	8 cut(s)	353, 461, 549, 875, 1442, 1526, 1893, 2387
TscAI	CASTG	3 cut(s)	157, 429, 1044
TseFI	GTSAC	5 cut(s)	144, 161, 1039, 1348, 2266
TseI	GCWGC	1 cut(s)	29
Tsp45I	GTSAC	5 cut(s)	144, 161, 1039, 1348, 2266
TspGWI	ACGGA	2 cut(s)	197, 423
TspRI	CASTG	3 cut(s)	157, 429, 1044
Vha464I	CTTAAG	1 cut(s)	1525
VneI	GTGCAC	2 cut(s)	1271, 2243
XapI	RAATTY	3 cut(s)	499, 1656, 1749
XceI	RCATGY	2 cut(s)	1711, 2386
XmaJI	CCTAGG	1 cut(s)	1883
XmnI	GAANNNNTTC	2 cut(s)	1740, 1985
XspI	CTAG	7 cut(s)	212, 519, 560, 1106, 1244, 1884, 1914
ZraI	GACGTC	1 cut(s)	2271
ZrmI	AGTACT	1 cut(s)	1634
Zsp2I	ATGCAT	2 cut(s)	737, 1390

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.