Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001609

Physical Location & Seq

Genomic Coordinates Forward (+)

36397914 .. 36407845

Gene Structure 9932 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1283600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGCCTGATGAAGAGGTACTAGTGGAACACCCTACAGATCAAGGAAGCCAAGATCATCAGGAAGTTTCTGATCATCATGATCATGATAAGGATCAACTCAAAGGGAAGAAACTTTCATGGCAAAAGTTGCGACGATACGACTCCTTGGACCTCGAGTCACGCAGTTTCACAGCTCCCCATGGCCATGCCTCCAAGGGCGCGGGCTGGGCAGTGATAATGCAACTGGCATTTCAGAGCATCGGAATAGTTTACGGGGACATTGGTACATCACCTCTGTATGTGTATGCGAGCACCTTCAGCAAAGGCATCAACCATGACGATGACATTTTGGGTGTTCTTTCTTTGATCTTTTACACTCTCACCTTAATCCCTCTCATTAAGTACGTTTTTATCGTCTTACGGGCCAACGATAATGGCGATGGGACGTTCGCACTATACTCTTTGCTGTGTCGATATGCCAAGGTTGGTTTGACTCCAAGTCAACAAGTGGAGGATCACGATGTTTCAAATTTTCAGCTTGAGTTGCCTACCAAAAGAGTAAAGAGGGCATCAAGGCTTAAATCTTCATTAGAGAACAGCCCATTCGCCAAAGTCTTTCTATTATGCGCCACCATGCTTGGTACGTCCATGGTCATTGGTGATGGTGTCCTCACTCCTTGCATCTCAGTTCTTGCGGCCGTTGGAGGAATCAAACGAGCCACATCTGCAATGACAGAAGATTGGGTTGTTTTGATATCAGTAGCCATCTTGATTGGCCTATTCATGGTTCAAAGATTTGGAACTGATAAAGTAGGCTACAGTTTTGCACCAATTATTTGCATTTGGTTTATCATGATTGCTGGAATTGGTGTCTACAACTTCATCAAGTTTGATCCGACGGTGGTCAAGGCTCTAAACCCACAATACATAGTAGATTACTTCAGGAGGAACAAAAAAGACGCTTGGATTTCTCTTGGTGGCATTGCCTTAGCAATAACAGGAACTGAAGCTTTATTTGCTGACGTGGGACACTTCACAGTGCGATCCATTCAAATAAGTATGTGCGCAGTTACTTATCCAACTCTAGTATTGGCATACACTGGACAAGCCTCTTTTCTTCGCAAGCACCATCTTCTTGTTGAAGATACCTTCTTCGAGTCAATACCAAAACCTTTGTATTGGCCCATGTTTGGAGTGGCTGTAATGGCATCAATCATAGCTAGTCAAGCTATGATTTCAGGGACTTTCTCAATAATCCAACAATCACTATCATTAGGGTGTTTCCCTCGTGTGAAAGTCGTGCACACATCGACCAAGTATGCCGGACAAGTTTACATTCCGGAAGTGAACTACCTTCTCATGTTAGCTTGTGTTGGAGTCACTTTAGGGTTTCGAAGCACTGAAGAGATCGGCAATGCATACGGTATAGCAGTGGTGTTTGTAATGACGCTTACATCATCCTTCCTAGTGCTAATCATGATCATGATATGGAAGACCAACATATTCCTCGTCATCTCATATGTTCTTGTCATTGGAAGTGTAGAGTTTCTGTATCTAAGTTCAGTGCTCTACAAATTTAACCAGGGCGGATATCTTCCCCTTGCGTTTGCCACAGTGTTGATGTTTGTAATGTTTGTGTGGAATGATGTGCATCAAAGGAAGTACTATTACGAGCTTAATCACAAAATTTCTCCCGAACAACTCAAAGAAATCGCCATTGATGCAAACTTTTCTCATTTGCCTGGCCTTGCCGTGTTCTATTCGGAACTTGTTCAAGGCATCCCGCCGATCTTCAATCATTATGTTGCGAATGTTCCTGCATTACACTCCGTCCTTGTTTTTGTCTCAATCAAATCGTTGCCTATAAGCAAGGTTCCGTTGGAAGAGCGCTTTCTTTTTCGGAGAGTAGAGCCTAACGATCTGAATGTGTTCAGATGTGTTGCAAGATACGGGTACACGGATGTTCGCAATGAGAACGAACCCTTCGAAGGATTGTTGGTGGAAAAGTTGAAAGAGTTCATAAAGAATAGTTTTTGGATATCTCAAGGAAATAATATGCACAGTACTAATGGGGAGATCAAGTTTGAGATCAAGGAAGAATTCAAAGACGGGACTTTGGCTAACGGTGGAGAGAATTTGAAGGAGGATTTGAAGATGCAAGTTGATGATCAAGATGACCAACAAGATTTGTTGGACAGAGATATTGAGGCAATAGACAAAGCATGGAGGTGGGGAGTTGTTCATTTGATTGGGGAAAATGAAGTGACGGCTGCCAAAGGAGCTGGTCTAGCGAAAAGAATTTTGATTGATTATGCTTACAATATCTTGAAGAGAAATTTGAGGCAGAGTGATAAAGTATTTGATATTCCTCATAAGCGCATGTTGAAAGTGGGCATGACTTATGAAATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

797

Amino Acids

89.47

Weight (kDa)

6.41

Isoelectric Point (pI)

32.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	75 - 560	4.4e-162	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	574 - 796	1.8e-28	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001609)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30541 FvH4_6g30550 FvH4_c6g00050 FvH4_c6g00050 FvH4_c6g00050
malus_domestica	MD03G1283500.v1.1 MD03G1283600.v1.1 MD10G1230200.v1.1 MD10G1230300.v1.1 MD11G1302600.v1.1 MD11G1302900.v1.1 MD11G1303100.v1.1
prunus_persica	Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1
pyrus_communis	pycom03g22450 pycom03g22460
rosa_chinensis	RchiOBHm_Chr2g0136981 RchiOBHm_Chr5g0020481 RchiOBHm_Chr6g0293251
rosa_laevigata	RLG00000011964 RLG00000033905
rosa_multiflora	Rmu_co8314359.1_g000001 Rmu_sc0001374.1_g000063 Rmu_sc0001374.1_g000064 Rmu_sc0021490.1_g000001 Rmu_ssc0000162.1_g000030
rosa_roxburghii	Rroxscaffold_2G00107800 Rroxscaffold_2G00107830 Rroxscaffold_7G00174310
rosa_rugosa	Rorug02G0336400 Rorug06G0233800 Rorug06G0233800 Rorug06G0233800
rosa_samantha	Rh2AG385800 Rh2BG392700 Rh2CG373000 Rh2DG047100 Rh2DG408800 Rh2DG408900 Rh3DG241600 Rh3DG283500 Rh6AG346700 Rh6BG354100 Rh6CG360600 Rh6DG347100
rosa_wichuraiana	Rw2G003360 Rw2G031570 Rw6G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1045
AccI	GTMKAC	1 cut(s)	852
AccII	CGCG	1 cut(s)	200
AccIII	TCCGGA	1 cut(s)	1318
AciI	CCGC	4 cut(s)	200, 674, 1566, 1763
AclWI	GGATC	4 cut(s)	99, 501, 866, 1017
AcoI	YGGCCR	2 cut(s)	181, 675
AcsI	RAATTY	8 cut(s)	508, 1553, 1665, 2078, 2113, 2277, 2314, 2385
AcuI	CTGAAG	4 cut(s)	280, 904, 1005, 1401
AfaI	GTAC	7 cut(s)	18, 265, 383, 622, 1643, 1934, 2044
AfeI	AGCGCT	1 cut(s)	1868
AfiI	CCNNNNNNNGG	3 cut(s)	763, 1169, 1967
AhdI	GACNNNNNGTC	2 cut(s)	154, 477
AhlI	ACTAGT	1 cut(s)	19
AjiI	CACGTC	1 cut(s)	1003
AjnI	CCWGG	2 cut(s)	1560, 1720
AluBI	AGCT	8 cut(s)	173, 517, 989, 1199, 1208, 1346, 1654, 2261
AluI	AGCT	8 cut(s)	173, 517, 989, 1199, 1208, 1346, 1654, 2261
Alw21I	GWGCWC	3 cut(s)	293, 1284, 1548
Alw26I	GTCTC	1 cut(s)	1828
Alw44I	GTGCAC	1 cut(s)	1280
AlwI	GGATC	4 cut(s)	99, 501, 866, 1017
AlwNI	CAGNNNCTG	1 cut(s)	983
Ama87I	CYCGRG	1 cut(s)	152
Aor13HI	TCCGGA	1 cut(s)	1318
Aor51HI	AGCGCT	1 cut(s)	1868
AoxI	GGCC	6 cut(s)	181, 402, 675, 754, 1160, 1723
ApaLI	GTGCAC	1 cut(s)	1280
ApeKI	GCWGC	1 cut(s)	2249
ApoI	RAATTY	8 cut(s)	508, 1553, 1665, 2078, 2113, 2277, 2314, 2385
Asp700I	GAANNNNTTC	3 cut(s)	1749, 1907, 1994
AspLEI	GCGC	5 cut(s)	200, 608, 1046, 1869, 2358
AspS9I	GGNCC	3 cut(s)	148, 402, 1161
AsuHPI	GGTGA	3 cut(s)	261, 352, 650
AsuII	TTCGAA	2 cut(s)	1372, 1965
AvaI	CYCGRG	1 cut(s)	152
AvaII	GGWCC	1 cut(s)	148
BaeGI	GKGCMC	1 cut(s)	1284
BalI	TGGCCA	1 cut(s)	183
BarI	GAAGNNNNNNTAC	2 cut(s)	1631, 1663
BauI	CACGAG	1 cut(s)	1266
BbsI	GAAGAC	1 cut(s)	1478
Bbv12I	GWGCWC	3 cut(s)	293, 1284, 1548
BbvI	GCAGC	1 cut(s)	2236
BccI	CCATC	4 cut(s)	413, 635, 752, 1116
BceAI	ACGGC	3 cut(s)	662, 1715, 2262
BciT130I	CCWGG	2 cut(s)	1562, 1722
BclI	TGATCA	4 cut(s)	70, 79, 1458, 2146
BcoDI	GTCTC	1 cut(s)	1828
BcuI	ACTAGT	1 cut(s)	19
BfaI	CTAG	5 cut(s)	20, 1064, 1200, 1445, 2267
BfmI	CTRYAG	2 cut(s)	33, 796
BfoI	RGCGCY	1 cut(s)	1870
BisI	GCNGC	2 cut(s)	675, 2250
BlsI	GCNGC	2 cut(s)	676, 2251
BmcAI	AGTACT	2 cut(s)	1643, 2044
Bme1390I	CCNGG	2 cut(s)	1562, 1722
Bme18I	GGWCC	1 cut(s)	148
BmeRI	GACNNNNNGTC	2 cut(s)	154, 477
BmeT110I	CYCGRG	1 cut(s)	152
BmgBI	CACGTC	1 cut(s)	1003
BmgT120I	GGNCC	3 cut(s)	148, 402, 1161
BmiI	GGNNCC	1 cut(s)	1854
BmrFI	CCNGG	2 cut(s)	1562, 1722
BmsI	GCATC	9 cut(s)	246, 315, 557, 669, 1196, 1639, 1690, 1767, 2124
BoxI	GACNNNNGTC	1 cut(s)	881
BpiI	GAAGAC	1 cut(s)	1478
Bpu10I	CCTNAGC	1 cut(s)	967
Bpu14I	TTCGAA	2 cut(s)	1372, 1965
BpuEI	CTTGAG	2 cut(s)	539, 2007
BsaBI	GATNNNNATC	2 cut(s)	90, 1629
BsaJI	CCNNGG	6 cut(s)	144, 178, 192, 459, 627, 1561
BsaWI	WCCGGW	1 cut(s)	1318
BsaXI	ACNNNNNCTCC	4 cut(s)	1164, 1194, 2045, 2075
Bsc4I	CCNNNNNNNGG	3 cut(s)	763, 1169, 1967
Bse1I	ACTGG	2 cut(s)	228, 1084
Bse3DI	GCAATG	4 cut(s)	714, 960, 1399, 1954
Bse8I	GATNNNNATC	2 cut(s)	90, 1629
BseAI	TCCGGA	1 cut(s)	1318
BseBI	CCWGG	2 cut(s)	1562, 1722
BseDI	CCNNGG	6 cut(s)	144, 178, 192, 459, 627, 1561
BseGI	GGATG	3 cut(s)	1436, 1758, 1945
BseJI	GATNNNNATC	2 cut(s)	90, 1629
BseLI	CCNNNNNNNGG	3 cut(s)	763, 1169, 1967
BseMI	GCAATG	4 cut(s)	714, 960, 1399, 1954
BseMII	CTCAG	1 cut(s)	678
BseNI	ACTGG	2 cut(s)	228, 1084
BseSI	GKGCMC	1 cut(s)	1284
BseX3I	CGGCCG	1 cut(s)	675
BseXI	GCAGC	1 cut(s)	2236
BseYI	CCCAGC	1 cut(s)	204
Bsh1236I	CGCG	1 cut(s)	200
Bsh1285I	CGRYCG	1 cut(s)	678
BshFI	GGCC	6 cut(s)	183, 404, 677, 756, 1162, 1725
BsiEI	CGRYCG	1 cut(s)	678
BsiHKAI	GWGCWC	3 cut(s)	293, 1284, 1548
BsiHKCI	CYCGRG	1 cut(s)	152
BsiSI	CCGG	2 cut(s)	1302, 1319
BslFI	GGGAC	5 cut(s)	269, 436, 1020, 1234, 2104
BslI	CCNNNNNNNGG	3 cut(s)	763, 1169, 1967
BsmAI	GTCTC	1 cut(s)	1828
BsmFI	GGGAC	5 cut(s)	269, 436, 1020, 1234, 2104
BsnI	GGCC	6 cut(s)	183, 404, 677, 756, 1162, 1725
BsoBI	CYCGRG	1 cut(s)	152
Bsp119I	TTCGAA	2 cut(s)	1372, 1965
Bsp1286I	GDGCHC	3 cut(s)	293, 1284, 1548
Bsp13I	TCCGGA	1 cut(s)	1318
Bsp19I	CCATGG	2 cut(s)	178, 627
BspACI	CCGC	4 cut(s)	200, 674, 1566, 1763
BspANI	GGCC	6 cut(s)	183, 404, 677, 756, 1162, 1725
BspCNI	CTCAG	1 cut(s)	677
BspEI	TCCGGA	1 cut(s)	1318
BspFNI	CGCG	1 cut(s)	200
BspHI	TCATGA	5 cut(s)	76, 82, 831, 1455, 1461
BspLI	GGNNCC	1 cut(s)	1854
BspPI	GGATC	4 cut(s)	99, 501, 866, 1017
BspQI	GCTCTTC	1 cut(s)	1857
BspT104I	TTCGAA	2 cut(s)	1372, 1965
BsrDI	GCAATG	4 cut(s)	714, 960, 1399, 1954
BsrI	ACTGG	2 cut(s)	228, 1084
BssECI	CCNNGG	6 cut(s)	144, 178, 192, 459, 627, 1561
BssSI	CACGAG	1 cut(s)	1266
BssT1I	CCWWGG	5 cut(s)	144, 178, 192, 459, 627
Bst2BI	CACGAG	1 cut(s)	1266
Bst2UI	CCWGG	2 cut(s)	1562, 1722
Bst4CI	ACNGT	7 cut(s)	800, 880, 1018, 1403, 1594, 2042, 2105
Bst6I	CTCTTC	4 cut(s)	6, 1377, 1857, 2303
BstAPI	GCANNNNNTGC	1 cut(s)	127
BstBI	TTCGAA	2 cut(s)	1372, 1965
BstC8I	GCNNGC	3 cut(s)	202, 289, 1103
BstDEI	CTNAG	3 cut(s)	664, 967, 1535
BstDSI	CCRYGG	2 cut(s)	178, 627
BstF5I	GGATG	3 cut(s)	1436, 1758, 1945
BstFNI	CGCG	1 cut(s)	200
BstH2I	RGCGCY	1 cut(s)	1870
BstHHI	GCGC	5 cut(s)	200, 608, 1046, 1869, 2358
BstMAI	GTCTC	1 cut(s)	1828
BstMCI	CGRYCG	1 cut(s)	678
BstNI	CCWGG	2 cut(s)	1562, 1722
BstNSI	RCATGY	1 cut(s)	2362
BstPAI	GACNNNNGTC	1 cut(s)	881
BstSCI	CCNGG	2 cut(s)	1560, 1720
BstSFI	CTRYAG	2 cut(s)	33, 796
BstSLI	GKGCMC	1 cut(s)	1284
BstUI	CGCG	1 cut(s)	200
BstV1I	GCAGC	1 cut(s)	2236
BstV2I	GAAGAC	1 cut(s)	1478
BstZI	CGGCCG	1 cut(s)	675
BsuRI	GGCC	6 cut(s)	183, 404, 677, 756, 1162, 1725
BtgI	CCRYGG	2 cut(s)	178, 627
BtgZI	GCGATG	1 cut(s)	432
BtrI	CACGTC	1 cut(s)	1003
BtsCI	GGATG	3 cut(s)	1436, 1758, 1945
BtsI	GCAGTG	2 cut(s)	216, 1416
BtsIMutI	CAGTG	7 cut(s)	216, 1023, 1077, 1377, 1416, 1548, 1599
Cac8I	GCNNGC	3 cut(s)	202, 289, 1103
CaiI	CAGNNNCTG	1 cut(s)	983
CciI	TCATGA	5 cut(s)	76, 82, 831, 1455, 1461
CfoI	GCGC	5 cut(s)	200, 608, 1046, 1869, 2358
Cfr13I	GGNCC	3 cut(s)	148, 402, 1161
CseI	GACGC	2 cut(s)	947, 1435
Csp6I	GTAC	7 cut(s)	17, 264, 382, 621, 1642, 1933, 2043
CspCI	CAANNNNNGTGG	2 cut(s)	598, 633
CviQI	GTAC	7 cut(s)	17, 264, 382, 621, 1642, 1933, 2043
DdeI	CTNAG	3 cut(s)	664, 967, 1535
DriI	GACNNNNNGTC	2 cut(s)	154, 477
EaeI	YGGCCR	2 cut(s)	181, 675
EagI	CGGCCG	1 cut(s)	675
Eam1104I	CTCTTC	4 cut(s)	6, 1377, 1857, 2303
Eam1105I	GACNNNNNGTC	2 cut(s)	154, 477
EarI	CTCTTC	4 cut(s)	6, 1377, 1857, 2303
EciI	GGCGGA	1 cut(s)	1581
EclXI	CGGCCG	1 cut(s)	675
Eco130I	CCWWGG	5 cut(s)	144, 178, 192, 459, 627
Eco32I	GATATC	3 cut(s)	735, 1571, 2019
Eco47I	GGWCC	1 cut(s)	148
Eco47III	AGCGCT	1 cut(s)	1868
Eco52I	CGGCCG	1 cut(s)	675
Eco57I	CTGAAG	4 cut(s)	280, 904, 1005, 1401
Eco88I	CYCGRG	1 cut(s)	152
EcoRI	GAATTC	1 cut(s)	2078
EcoRII	CCWGG	2 cut(s)	1560, 1720
EcoRV	GATATC	3 cut(s)	735, 1571, 2019
EcoT14I	CCWWGG	5 cut(s)	144, 178, 192, 459, 627
EcoT22I	ATGCAT	1 cut(s)	1399
ErhI	CCWWGG	5 cut(s)	144, 178, 192, 459, 627
FaqI	GGGAC	5 cut(s)	269, 436, 1020, 1234, 2104
FauI	CCCGC	2 cut(s)	193, 1770
FauNDI	CATATG	1 cut(s)	1498
FbaI	TGATCA	4 cut(s)	70, 79, 1458, 2146
FblI	GTMKAC	1 cut(s)	852
Fnu4HI	GCNGC	2 cut(s)	675, 2250
FokI	GGATG	3 cut(s)	1423, 1745, 1952
Fsp4HI	GCNGC	2 cut(s)	675, 2250
FspBI	CTAG	5 cut(s)	20, 1064, 1200, 1445, 2267
FspI	TGCGCA	1 cut(s)	1045
GlaI	GCGC	5 cut(s)	199, 607, 1045, 1868, 2357
GluI	GCNGC	2 cut(s)	675, 2250
GsaI	CCCAGC	1 cut(s)	208
HaeII	RGCGCY	1 cut(s)	1870
HaeIII	GGCC	6 cut(s)	183, 404, 677, 756, 1162, 1725
HapII	CCGG	2 cut(s)	1302, 1319
HgaI	GACGC	2 cut(s)	947, 1435
HhaI	GCGC	5 cut(s)	200, 608, 1046, 1869, 2358
Hin6I	GCGC	5 cut(s)	198, 606, 1044, 1867, 2356
HinP1I	GCGC	5 cut(s)	198, 606, 1044, 1867, 2356
HincII	GTYRAC	1 cut(s)	482
HindII	GTYRAC	1 cut(s)	482
HindIII	AAGCTT	1 cut(s)	987
HinfI	GANTC	6 cut(s)	140, 155, 472, 687, 1136, 1356
HpaII	CCGG	2 cut(s)	1302, 1319
HphI	GGTGA	3 cut(s)	261, 352, 650
Hpy166II	GTNNAC	7 cut(s)	250, 482, 853, 1282, 1312, 1327, 1935
Hpy188I	TCNGA	8 cut(s)	70, 234, 242, 876, 1744, 1881, 1902, 1913
Hpy8I	GTNNAC	7 cut(s)	250, 482, 853, 1282, 1312, 1327, 1935
Hpy99I	CGWCG	2 cut(s)	135, 880
HpyAV	CCTTC	7 cut(s)	304, 1138, 1343, 1450, 1961, 1972, 2113
HpyCH4III	ACNGT	7 cut(s)	800, 880, 1018, 1403, 1594, 2042, 2105
HpyCH4IV	ACGT	4 cut(s)	384, 425, 623, 1002
HpyF3I	CTNAG	3 cut(s)	664, 967, 1535
HpySE526I	ACGT	4 cut(s)	384, 425, 623, 1002
HspAI	GCGC	5 cut(s)	198, 606, 1044, 1867, 2356
Kpn2I	TCCGGA	1 cut(s)	1318
Ksp22I	TGATCA	4 cut(s)	70, 79, 1458, 2146
LguI	GCTCTTC	1 cut(s)	1857
LmnI	GCTCC	2 cut(s)	178, 2258
Lsp1109I	GCAGC	1 cut(s)	2236
LweI	GCATC	9 cut(s)	246, 315, 557, 669, 1196, 1639, 1690, 1767, 2124
MaeI	CTAG	5 cut(s)	20, 1064, 1200, 1445, 2267
MaeII	ACGT	4 cut(s)	384, 425, 623, 1002
MaeIII	GTNAC	4 cut(s)	156, 1048, 1357, 2242
MhlI	GDGCHC	3 cut(s)	293, 1284, 1548
MlsI	TGGCCA	1 cut(s)	183
MluNI	TGGCCA	1 cut(s)	183
MlyI	GAGTC	5 cut(s)	134, 164, 466, 1145, 1365
MmeI	TCCRAC	7 cut(s)	661, 899, 1082, 1261, 1333, 1839, 2151
Mox20I	TGGCCA	1 cut(s)	183
Mph1103I	ATGCAT	1 cut(s)	1399
MroI	TCCGGA	1 cut(s)	1318
MroXI	GAANNNNTTC	3 cut(s)	1749, 1907, 1994
MscI	TGGCCA	1 cut(s)	183
MseI	TTAA	5 cut(s)	365, 378, 558, 1557, 1656
MslI	CAYNNNNRTG	6 cut(s)	81, 183, 318, 1255, 1460, 2206
Msp20I	TGGCCA	1 cut(s)	183
MspI	CCGG	2 cut(s)	1302, 1319
MspR9I	CCNGG	2 cut(s)	1562, 1722
MvaI	CCWGG	2 cut(s)	1562, 1722
MvnI	CGCG	1 cut(s)	200
NcoI	CCATGG	2 cut(s)	178, 627
NdeI	CATATG	1 cut(s)	1498
NlaIV	GGNNCC	1 cut(s)	1854
NmuCI	GTSAC	3 cut(s)	156, 1357, 2242
NsbI	TGCGCA	1 cut(s)	1045
NsiI	ATGCAT	1 cut(s)	1399
NspI	RCATGY	1 cut(s)	2362
NspV	TTCGAA	2 cut(s)	1372, 1965
PaeR7I	CTCGAG	1 cut(s)	152
PagI	TCATGA	5 cut(s)	76, 82, 831, 1455, 1461
PciSI	GCTCTTC	1 cut(s)	1857
PcsI	WCGNNNNNNNCGW	3 cut(s)	390, 414, 1962
PdmI	GAANNNNTTC	3 cut(s)	1749, 1907, 1994
PfeI	GAWTC	1 cut(s)	687
PkrI	GCNGC	2 cut(s)	676, 2251
PleI	GAGTC	5 cut(s)	134, 163, 466, 1144, 1364
PpsI	GAGTC	5 cut(s)	134, 163, 466, 1144, 1364
PshAI	GACNNNNGTC	1 cut(s)	881
Psp6I	CCWGG	2 cut(s)	1560, 1720
PspFI	CCCAGC	1 cut(s)	204
PspGI	CCWGG	2 cut(s)	1560, 1720
PspN4I	GGNNCC	1 cut(s)	1854
PspPI	GGNCC	3 cut(s)	148, 402, 1161
PspXI	VCTCGAGB	1 cut(s)	152
PstNI	CAGNNNCTG	1 cut(s)	983
RsaI	GTAC	7 cut(s)	18, 265, 383, 622, 1643, 1934, 2044
RsaNI	GTAC	7 cut(s)	17, 264, 382, 621, 1642, 1933, 2043
RseI	CAYNNNNRTG	6 cut(s)	81, 183, 318, 1255, 1460, 2206
SapI	GCTCTTC	1 cut(s)	1857
SaqAI	TTAA	5 cut(s)	365, 378, 558, 1557, 1656
SatI	GCNGC	2 cut(s)	675, 2250
Sau96I	GGNCC	3 cut(s)	148, 402, 1161
ScaI	AGTACT	2 cut(s)	1643, 2044
SchI	GAGTC	5 cut(s)	134, 164, 466, 1145, 1365
ScrFI	CCNGG	2 cut(s)	1562, 1722
SduI	GDGCHC	3 cut(s)	293, 1284, 1548
SfaNI	GCATC	9 cut(s)	246, 315, 557, 669, 1196, 1639, 1690, 1767, 2124
SfcI	CTRYAG	2 cut(s)	33, 796
Sfr274I	CTCGAG	1 cut(s)	152
SfuI	TTCGAA	2 cut(s)	1372, 1965
SinI	GGWCC	1 cut(s)	148
SlaI	CTCGAG	1 cut(s)	152
SmiMI	CAYNNNNRTG	6 cut(s)	81, 183, 318, 1255, 1460, 2206
SmlI	CTYRAG	3 cut(s)	152, 518, 2022
SmoI	CTYRAG	3 cut(s)	152, 518, 2022
SpeI	ACTAGT	1 cut(s)	19
SsiI	CCGC	4 cut(s)	200, 674, 1566, 1763
SspMI	CTAG	5 cut(s)	20, 1064, 1200, 1445, 2267
StyD4I	CCNGG	2 cut(s)	1560, 1720
StyI	CCWWGG	5 cut(s)	144, 178, 192, 459, 627
TaaI	ACNGT	7 cut(s)	800, 880, 1018, 1403, 1594, 2042, 2105
TaiI	ACGT	4 cut(s)	387, 428, 626, 1005
TaqI	TCGA	6 cut(s)	153, 451, 1134, 1289, 1372, 1965
TatI	WGTACW	2 cut(s)	1641, 2042
TauI	GCSGC	1 cut(s)	677
TfiI	GAWTC	1 cut(s)	687
Tru1I	TTAA	5 cut(s)	365, 378, 558, 1557, 1656
Tru9I	TTAA	5 cut(s)	365, 378, 558, 1557, 1656
TscAI	CASTG	7 cut(s)	216, 1023, 1084, 1384, 1416, 1548, 1599
TseFI	GTSAC	3 cut(s)	156, 1357, 2242
TseI	GCWGC	1 cut(s)	2249
Tsp45I	GTSAC	3 cut(s)	156, 1357, 2242
TspDTI	ATGAA	8 cut(s)	24, 105, 555, 751, 850, 1987, 2210, 2253
TspGWI	ACGGA	3 cut(s)	1798, 1845, 1952
TspRI	CASTG	7 cut(s)	216, 1023, 1084, 1384, 1416, 1548, 1599
VneI	GTGCAC	1 cut(s)	1280
VpaK11BI	GGWCC	1 cut(s)	148
XapI	RAATTY	8 cut(s)	508, 1553, 1665, 2078, 2113, 2277, 2314, 2385
XceI	RCATGY	1 cut(s)	2362
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1171
XhoI	CTCGAG	1 cut(s)	152
XmiI	GTMKAC	1 cut(s)	852
XmnI	GAANNNNTTC	3 cut(s)	1749, 1907, 1994
XspI	CTAG	5 cut(s)	20, 1064, 1200, 1445, 2267
ZrmI	AGTACT	2 cut(s)	1643, 2044
Zsp2I	ATGCAT	1 cut(s)	1399

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.