Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0001609

Physical Location & Seq

Genomic Coordinates Reverse (-)

55675325 .. 55679412

Gene Structure 4088 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG392700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGGCTACAGATGTAATACTGCAAAACACTGTGGAAGAGGAGGAAAGCCAGCATGGGGATCAAGTCTCTGAACAGCTCAAAGAGAATCAACTAGTTTCAAGCCAAAAGTTTCAAAGATTTGACTCCTTGGACATCGAGTCTGGCGCTGATCACAGGCACTCCAAGGAAATGACGTGGTCCGTAATACTGACCCTGGCATTTCAGAGCATTGGAGTTGTATATGGAGATCTTGGTACTTCACCACTTTATGTGTTCTCGAGCACCTTCCCCAATGGAATCAAAGATAAAGATGACATCTTGGGTGTTCTTTCTTTGATTCTTTATACAGTCACATTAATCCCCTTGATTAAGTATGTTCTCATCGTCCTACAAGCAAATGATAACGGCGATGGAGGGACATTTGCACTGTACTCTCTCTTATGTCGACACGCAAAGATTAGTTTGATTCCGAGCCAGCAAGAAGAGGATTCCAATGTTTCAAATTTCAAACTTGAAGTTCCTAGCAGACGTCTAAAAATGGCATCAACTCTTAAATCTGCACTGGAGAACAGTCACTTTGCCAAAGTCTTCTTACTCTTTGCCACAATGCTTGGCACTTCCATGGTCATTGGCGATGGTGTCCTCACTCCTTGCATCTCAGTTCTATCAGCTGTGGTTGGCATCAAGGGAGCTACGTCTGCAATGACAGAAGATATGATTGTGTGGATATCGGTAGCCATCTTGATAGTCCTTTTCATCATCCAAAGATTTGGAACTGACAAAGTGGGCTACAGTTTTGCTCCAATACTTTGTATTTGGTTTCTATTCATTGGTGTGATCGGCTTGTACAATTTCATCAAATTTGATCCAATGGTTATCAAAGCCATAAATCCACGGTACATAGTGGATTACTTCATACGGAACAAGAAAGAAGCTTGGATCTCCCTTGGTGGCGTTATTCTATGCATAACAGGAGCTGAAGCTCTGTTTGCTGATGTTGGCCACTTCACTGTTCGATCCATACAAATTAGTACATGCACGATTGTTTACCCATCTGTAGTGTTGGCTTACATTGGGCAAGCCTCTTTTCTTCGCAAGCATGCCGATCTTGTTGCTGATACCTTCTTCCAGTCTATACCAAGACCTATATATTGGCCTATGTTTGTTGTTGCTGTATTAGCATCAATAATAGCAAGTCAATCCCTGATTTCTGCGACCTTTTCCATAATTCAACAATCTCTGTCATTAGGGTGTTTCCCTCGTGTGAAAGTCGTGCACACATCATCCAAGTATGAAGGACAAGTTTATATTCCGGAGGTCAACTTCATTCTTATGTTGGCCTGTGTAGGGGTCACCTTGGCTTTTAGGACCACTACAAAGATTGGAAATGCACTAGGTATAGCAGTGGTGTTTGTAATGACACTAACTTCATCATTCCTCGTGCTAATTATGATAATGATATGGAAAACCCACATACTTCTCATTATCTCATATGTTCTTATTATTGGAAGTATGGAGCTTCTGTATTTAAGTTCAGTCCTTTACAAGTTTGACCAAGGGGGATATCTTCCAGTAGCCTTTGCCACTCTAATGATGGCTATAATGTTTGTTTGGAATGATGTTTATCGAAGAAAGTACTACTATGAGGTTGAACAGAAAATATCTCCTCAAAAGTTCAAGCAGATTGCTGATGATAGAAAATTTTGTCATATCCCTGGCCTCGCCATATTCTACTCGGAACTTGTTCATGGCATCCCACCCATTTTCAATCACTACTTAGCCAACGTGCCTGCATTGCACTCAGTCCTTGTTTTTGTCTCCATCAGACATTTACCCATAAGCAAGGTTCCGATGGAAGAGCGTCTCCTCTTCCGCCGAGTAGAGCCCAAGGAGCTTAATGTATTTCGATGTGTTGCGAGGTACGGGTATACAGATCTCTACCATGTGCAAGAACCCTTTGAAGAATTGTTGGTAAAAAAGTTGAAAGAGTTCTTGAAAGATGGTTGTTTCATACCCCAACAAACAAGTAATAATGGAGAAGTGTTTGAAAAGCAAGAAGAATTAAATGATGGATTGGTTGACAACGAGAATGAATTTGTTGACATACAACAAGAAGAACTGATGGAGGCTGAGGTTCAACTTGAAGCAATAGATAAAGCATGGAGTTCAGGGGTTATTCACCTCATTGGTGAAAATGAAGTCATAGCTGCCAAAGGATCTGGTATAGCCAAAAGAATTTTGATTGATTATGCTTACAATTTTTTGAAGAGCAATTTGAGGCAGAGAGGCGAATTGTTTGAAATTCCTCGCAAGCGCATGCTAAAAGTGGGTATGACCTATGAACTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

775

Amino Acids

87.27

Weight (kDa)

6.1

Isoelectric Point (pI)

32.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	65 - 552	7.1e-162	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	565 - 775	5.7e-28	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001609)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30541 FvH4_6g30550 FvH4_c6g00050 FvH4_c6g00050 FvH4_c6g00050
malus_domestica	MD03G1283500.v1.1 MD03G1283600.v1.1 MD10G1230200.v1.1 MD10G1230300.v1.1 MD11G1302600.v1.1 MD11G1302900.v1.1 MD11G1303100.v1.1
prunus_persica	Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1
pyrus_communis	pycom03g22450 pycom03g22460
rosa_chinensis	RchiOBHm_Chr2g0136981 RchiOBHm_Chr5g0020481 RchiOBHm_Chr6g0293251
rosa_laevigata	RLG00000011964 RLG00000033905
rosa_multiflora	Rmu_co8314359.1_g000001 Rmu_sc0001374.1_g000063 Rmu_sc0001374.1_g000064 Rmu_sc0021490.1_g000001 Rmu_ssc0000162.1_g000030
rosa_roxburghii	Rroxscaffold_2G00107800 Rroxscaffold_2G00107830 Rroxscaffold_7G00174310
rosa_rugosa	Rorug02G0336400 Rorug06G0233800 Rorug06G0233800 Rorug06G0233800
rosa_samantha	Rh2AG385800 Rh2BG392700 Rh2CG373000 Rh2DG047100 Rh2DG408800 Rh2DG408900 Rh3DG241600 Rh3DG283500 Rh6AG346700 Rh6BG354100 Rh6CG360600 Rh6DG347100
rosa_wichuraiana	Rw2G003360 Rw2G031570 Rw6G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	511
AccI	GTMKAC	2 cut(s)	424, 1907
AccIII	TCCGGA	1 cut(s)	1291
AciI	CCGC	1 cut(s)	1852
AclWI	GGATC	5 cut(s)	66, 839, 926, 990, 2203
AcoI	YGGCCR	1 cut(s)	979
AcsI	RAATTY	6 cut(s)	481, 839, 1679, 2072, 2214, 2280
AcuI	CTGAAG	1 cut(s)	978
AcyI	GRCGYC	1 cut(s)	508
AfaI	GTAC	7 cut(s)	235, 410, 827, 878, 1012, 1616, 1901
AfiI	CCNNNNNNNGG	2 cut(s)	55, 1326
AhdI	GACNNNNNGTC	1 cut(s)	136
AhlI	ACTAGT	1 cut(s)	91
AjiI	CACGTC	1 cut(s)	174
AjnI	CCWGG	2 cut(s)	192, 1693
AluBI	AGCT	9 cut(s)	76, 650, 671, 914, 956, 962, 1498, 1873, 2186
AluI	AGCT	9 cut(s)	76, 650, 671, 914, 956, 962, 1498, 1873, 2186
Alw21I	GWGCWC	2 cut(s)	263, 1257
Alw26I	GTCTC	3 cut(s)	70, 1801, 1847
Alw44I	GTGCAC	1 cut(s)	1253
AlwI	GGATC	5 cut(s)	66, 839, 926, 990, 2203
AlwNI	CAGNNNCTG	1 cut(s)	956
Ama87I	CYCGRG	1 cut(s)	256
Aor13HI	TCCGGA	1 cut(s)	1291
AoxI	GGCC	4 cut(s)	979, 1133, 1317, 1696
ApaLI	GTGCAC	1 cut(s)	1253
ApeKI	GCWGC	1 cut(s)	2186
ApoI	RAATTY	6 cut(s)	481, 839, 1679, 2072, 2214, 2280
AseI	ATTAAT	1 cut(s)	335
Asp700I	GAANNNNTTC	2 cut(s)	1722, 1967
AspLEI	GCGC	2 cut(s)	146, 2295
AspS9I	GGNCC	2 cut(s)	177, 1347
AsuHPI	GGTGA	4 cut(s)	231, 1324, 2150, 2180
AvaI	CYCGRG	1 cut(s)	256
AvaII	GGWCC	2 cut(s)	177, 1347
BaeGI	GKGCMC	1 cut(s)	1257
BalI	TGGCCA	1 cut(s)	981
BanII	GRGCYC	1 cut(s)	1866
BauI	CACGAG	2 cut(s)	1239, 1418
BbsI	GAAGAC	1 cut(s)	559
Bbv12I	GWGCWC	2 cut(s)	263, 1257
BbvCI	CCTCAGC	1 cut(s)	2109
BbvI	GCAGC	1 cut(s)	2173
BceAI	ACGGC	1 cut(s)	400
BcgI	CGANNNNNNTGC	2 cut(s)	1073, 1107
BciT130I	CCWGG	2 cut(s)	194, 1695
BclI	TGATCA	1 cut(s)	148
BcoDI	GTCTC	3 cut(s)	70, 1801, 1847
BcuI	ACTAGT	1 cut(s)	91
BfaI	CTAG	3 cut(s)	92, 501, 1373
BfmI	CTRYAG	3 cut(s)	6, 769, 1035
BfoI	RGCGCY	1 cut(s)	147
BglII	AGATCT	2 cut(s)	226, 1912
BisI	GCNGC	1 cut(s)	2187
BlsI	GCNGC	1 cut(s)	2188
BmcAI	AGTACT	1 cut(s)	1616
Bme1390I	CCNGG	2 cut(s)	194, 1695
Bme18I	GGWCC	2 cut(s)	177, 1347
BmeRI	GACNNNNNGTC	1 cut(s)	136
BmeT110I	CYCGRG	1 cut(s)	256
BmgBI	CACGTC	1 cut(s)	174
BmgT120I	GGNCC	2 cut(s)	177, 1347
BmiI	GGNNCC	1 cut(s)	1827
BmrFI	CCNGG	2 cut(s)	194, 1695
BmsI	GCATC	5 cut(s)	530, 642, 669, 1169, 1740
BpiI	GAAGAC	1 cut(s)	559
BpmI	CTGGAG	1 cut(s)	563
Bpu10I	CCTNAGC	1 cut(s)	2109
BsaBI	GATNNNNATC	1 cut(s)	1602
BsaHI	GRCGYC	1 cut(s)	508
BsaWI	WCCGGW	1 cut(s)	1291
BsaXI	ACNNNNNCTCC	4 cut(s)	143, 173, 1487, 1517
Bsc4I	CCNNNNNNNGG	2 cut(s)	55, 1326
Bse1I	ACTGG	3 cut(s)	546, 1108, 1550
Bse3DI	GCAATG	2 cut(s)	687, 1772
Bse8I	GATNNNNATC	1 cut(s)	1602
BseAI	TCCGGA	1 cut(s)	1291
BseBI	CCWGG	2 cut(s)	194, 1695
BseGI	GGATG	3 cut(s)	738, 1262, 1731
BseJI	GATNNNNATC	1 cut(s)	1602
BseLI	CCNNNNNNNGG	2 cut(s)	55, 1326
BseMI	GCAATG	2 cut(s)	687, 1772
BseMII	CTCAG	3 cut(s)	651, 1794, 2100
BseNI	ACTGG	3 cut(s)	546, 1108, 1550
BseRI	GAGGAG	3 cut(s)	53, 1635, 1835
BseSI	GKGCMC	1 cut(s)	1257
BseXI	GCAGC	1 cut(s)	2173
BsgI	GTGCAG	1 cut(s)	522
BshFI	GGCC	4 cut(s)	981, 1135, 1319, 1698
BsiHKAI	GWGCWC	2 cut(s)	263, 1257
BsiHKCI	CYCGRG	1 cut(s)	256
BsiSI	CCGG	1 cut(s)	1292
BslFI	GGGAC	1 cut(s)	409
BslI	CCNNNNNNNGG	2 cut(s)	55, 1326
BsmAI	GTCTC	3 cut(s)	70, 1801, 1847
BsmBI	CGTCTC	1 cut(s)	1847
BsmFI	GGGAC	1 cut(s)	409
BsnI	GGCC	4 cut(s)	981, 1135, 1319, 1698
BsoBI	CYCGRG	1 cut(s)	256
Bsp1286I	GDGCHC	3 cut(s)	263, 1257, 1866
Bsp13I	TCCGGA	1 cut(s)	1291
Bsp1407I	TGTACA	1 cut(s)	825
Bsp19I	CCATGG	1 cut(s)	600
BspACI	CCGC	1 cut(s)	1852
BspANI	GGCC	4 cut(s)	981, 1135, 1319, 1698
BspCNI	CTCAG	3 cut(s)	650, 1793, 2101
BspEI	TCCGGA	1 cut(s)	1291
BspLI	GGNNCC	1 cut(s)	1827
BspPI	GGATC	5 cut(s)	66, 839, 926, 990, 2203
BspQI	GCTCTTC	2 cut(s)	1830, 2240
BsrDI	GCAATG	2 cut(s)	687, 1772
BsrGI	TGTACA	1 cut(s)	825
BsrI	ACTGG	3 cut(s)	546, 1108, 1550
BssNAI	GTATAC	1 cut(s)	1908
BssNI	GRCGYC	1 cut(s)	508
BssSI	CACGAG	2 cut(s)	1239, 1418
BssT1I	CCWWGG	7 cut(s)	126, 162, 600, 925, 1335, 1534, 1866
Bst1107I	GTATAC	1 cut(s)	1908
Bst2BI	CACGAG	2 cut(s)	1239, 1418
Bst2UI	CCWGG	2 cut(s)	194, 1695
Bst4CI	ACNGT	7 cut(s)	31, 328, 408, 551, 773, 876, 991
Bst6I	CTCTTC	5 cut(s)	30, 456, 1830, 1853, 2240
BstACI	GRCGYC	1 cut(s)	508
BstAUI	TGTACA	1 cut(s)	825
BstC8I	GCNNGC	8 cut(s)	50, 455, 1059, 1076, 1080, 1770, 2291, 2297
BstDEI	CTNAG	4 cut(s)	637, 1756, 1780, 2109
BstDSI	CCRYGG	2 cut(s)	600, 872
BstEII	GGTNACC	1 cut(s)	1330
BstF5I	GGATG	3 cut(s)	738, 1262, 1731
BstH2I	RGCGCY	1 cut(s)	147
BstHHI	GCGC	2 cut(s)	146, 2295
BstMAI	GTCTC	3 cut(s)	70, 1801, 1847
BstMWI	GCNNNNNNNGC	5 cut(s)	677, 968, 1157, 1774, 1870
BstNI	CCWGG	2 cut(s)	194, 1695
BstNSI	RCATGY	3 cut(s)	1017, 1082, 2299
BstPI	GGTNACC	1 cut(s)	1330
BstSCI	CCNGG	2 cut(s)	192, 1693
BstSFI	CTRYAG	3 cut(s)	6, 769, 1035
BstSLI	GKGCMC	1 cut(s)	1257
BstV1I	GCAGC	1 cut(s)	2173
BstV2I	GAAGAC	1 cut(s)	559
BstX2I	RGATCY	4 cut(s)	226, 918, 1912, 2195
BstXI	CCANNNNNNTGG	1 cut(s)	749
BstYI	RGATCY	4 cut(s)	226, 918, 1912, 2195
BstZ17I	GTATAC	1 cut(s)	1908
BsuRI	GGCC	4 cut(s)	981, 1135, 1319, 1698
BtgI	CCRYGG	2 cut(s)	600, 872
BtgZI	GCGATG	2 cut(s)	402, 627
BtrI	CACGTC	1 cut(s)	174
BtsCI	GGATG	3 cut(s)	738, 1262, 1731
BtsI	GCAGTG	1 cut(s)	1389
BtsIMutI	CAGTG	5 cut(s)	27, 404, 539, 987, 1389
Cac8I	GCNNGC	8 cut(s)	50, 455, 1059, 1076, 1080, 1770, 2291, 2297
CaiI	CAGNNNCTG	1 cut(s)	956
CfoI	GCGC	2 cut(s)	146, 2295
Cfr13I	GGNCC	2 cut(s)	177, 1347
CseI	GACGC	1 cut(s)	1829
Csp6I	GTAC	7 cut(s)	234, 409, 826, 877, 1011, 1615, 1900
CspCI	CAANNNNNGTGG	2 cut(s)	571, 606
CviAII	CATG	8 cut(s)	53, 601, 1014, 1079, 1727, 1922, 2139, 2296
CviQI	GTAC	7 cut(s)	234, 409, 826, 877, 1011, 1615, 1900
DdeI	CTNAG	4 cut(s)	637, 1756, 1780, 2109
DriI	GACNNNNNGTC	1 cut(s)	136
EaeI	YGGCCR	1 cut(s)	979
Eam1104I	CTCTTC	5 cut(s)	30, 456, 1830, 1853, 2240
Eam1105I	GACNNNNNGTC	1 cut(s)	136
EarI	CTCTTC	5 cut(s)	30, 456, 1830, 1853, 2240
EciI	GGCGGA	1 cut(s)	1841
Eco130I	CCWWGG	7 cut(s)	126, 162, 600, 925, 1335, 1534, 1866
Eco24I	GRGCYC	1 cut(s)	1866
Eco32I	GATATC	2 cut(s)	708, 1544
Eco47I	GGWCC	2 cut(s)	177, 1347
Eco57I	CTGAAG	1 cut(s)	978
Eco88I	CYCGRG	1 cut(s)	256
Eco91I	GGTNACC	1 cut(s)	1330
EcoO65I	GGTNACC	1 cut(s)	1330
EcoRII	CCWGG	2 cut(s)	192, 1693
EcoRV	GATATC	2 cut(s)	708, 1544
EcoT14I	CCWWGG	7 cut(s)	126, 162, 600, 925, 1335, 1534, 1866
EcoT22I	ATGCAT	1 cut(s)	947
EcoT38I	GRGCYC	1 cut(s)	1866
ErhI	CCWWGG	7 cut(s)	126, 162, 600, 925, 1335, 1534, 1866
Esp3I	CGTCTC	1 cut(s)	1847
FaeI	CATG	8 cut(s)	56, 604, 1017, 1082, 1730, 1925, 2142, 2299
FaqI	GGGAC	1 cut(s)	409
FatI	CATG	8 cut(s)	52, 600, 1013, 1078, 1726, 1921, 2138, 2295
FauNDI	CATATG	1 cut(s)	1471
FbaI	TGATCA	1 cut(s)	148
FblI	GTMKAC	2 cut(s)	424, 1907
Fnu4HI	GCNGC	1 cut(s)	2187
FokI	GGATG	3 cut(s)	725, 1249, 1718
FriOI	GRGCYC	1 cut(s)	1866
Fsp4HI	GCNGC	1 cut(s)	2187
FspBI	CTAG	3 cut(s)	92, 501, 1373
GlaI	GCGC	2 cut(s)	145, 2294
GluI	GCNGC	1 cut(s)	2187
GsuI	CTGGAG	1 cut(s)	563
HaeII	RGCGCY	1 cut(s)	147
HaeIII	GGCC	4 cut(s)	981, 1135, 1319, 1698
HapII	CCGG	1 cut(s)	1292
HgaI	GACGC	1 cut(s)	1829
HhaI	GCGC	2 cut(s)	146, 2295
Hin1I	GRCGYC	1 cut(s)	508
Hin1II	CATG	8 cut(s)	56, 604, 1017, 1082, 1730, 1925, 2142, 2299
Hin6I	GCGC	2 cut(s)	144, 2293
HinP1I	GCGC	2 cut(s)	144, 2293
HincII	GTYRAC	4 cut(s)	425, 1300, 2059, 2080
HindII	GTYRAC	4 cut(s)	425, 1300, 2059, 2080
HindIII	AAGCTT	1 cut(s)	912
HinfI	GANTC	7 cut(s)	85, 122, 137, 276, 316, 445, 467
HpaII	CCGG	1 cut(s)	1292
HphI	GGTGA	4 cut(s)	231, 1324, 2150, 2180
Hpy166II	GTNNAC	7 cut(s)	425, 1027, 1255, 1300, 1908, 2059, 2080
Hpy188I	TCNGA	6 cut(s)	70, 204, 450, 1717, 1805, 1830
Hpy188III	TCNNGA	4 cut(s)	256, 721, 1292, 1972
Hpy8I	GTNNAC	7 cut(s)	425, 1027, 1255, 1300, 1908, 2059, 2080
HpyAV	CCTTC	3 cut(s)	274, 1111, 1268
HpyCH4III	ACNGT	7 cut(s)	31, 328, 408, 551, 773, 876, 991
HpyCH4IV	ACGT	4 cut(s)	173, 508, 674, 1764
HpyF10VI	GCNNNNNNNGC	5 cut(s)	677, 968, 1157, 1774, 1870
HpyF3I	CTNAG	4 cut(s)	637, 1756, 1780, 2109
HpySE526I	ACGT	4 cut(s)	173, 508, 674, 1764
Hsp92I	GRCGYC	1 cut(s)	508
Hsp92II	CATG	8 cut(s)	56, 604, 1017, 1082, 1730, 1925, 2142, 2299
HspAI	GCGC	2 cut(s)	144, 2293
Kpn2I	TCCGGA	1 cut(s)	1291
Ksp22I	TGATCA	1 cut(s)	148
LguI	GCTCTTC	2 cut(s)	1830, 2240
LmnI	GCTCC	5 cut(s)	668, 784, 953, 1495, 1870
Lsp1109I	GCAGC	1 cut(s)	2173
LweI	GCATC	5 cut(s)	530, 642, 669, 1169, 1740
MaeI	CTAG	3 cut(s)	92, 501, 1373
MaeII	ACGT	4 cut(s)	173, 508, 674, 1764
MaeIII	GTNAC	3 cut(s)	328, 551, 1330
MflI	RGATCY	4 cut(s)	226, 918, 1912, 2195
MhlI	GDGCHC	3 cut(s)	263, 1257, 1866
MlsI	TGGCCA	1 cut(s)	981
MluNI	TGGCCA	1 cut(s)	981
MlyI	GAGTC	2 cut(s)	116, 146
Mox20I	TGGCCA	1 cut(s)	981
Mph1103I	ATGCAT	1 cut(s)	947
MroI	TCCGGA	1 cut(s)	1291
MroXI	GAANNNNTTC	2 cut(s)	1722, 1967
MscI	TGGCCA	1 cut(s)	981
MseI	TTAA	6 cut(s)	335, 348, 531, 1508, 1875, 2042
MslI	CAYNNNNRTG	5 cut(s)	599, 812, 1228, 1310, 1568
Msp20I	TGGCCA	1 cut(s)	981
MspA1I	CMGCKG	1 cut(s)	650
MspI	CCGG	1 cut(s)	1292
MspR9I	CCNGG	2 cut(s)	194, 1695
MvaI	CCWGG	2 cut(s)	194, 1695
MwoI	GCNNNNNNNGC	5 cut(s)	677, 968, 1157, 1774, 1870
NcoI	CCATGG	1 cut(s)	600
NdeI	CATATG	1 cut(s)	1471
NlaIII	CATG	8 cut(s)	56, 604, 1017, 1082, 1730, 1925, 2142, 2299
NlaIV	GGNNCC	1 cut(s)	1827
NmeAIII	GCCGAG	1 cut(s)	1880
NmuCI	GTSAC	3 cut(s)	328, 551, 1330
NsiI	ATGCAT	1 cut(s)	947
NspI	RCATGY	3 cut(s)	1017, 1082, 2299
PaeI	GCATGC	2 cut(s)	1082, 2299
PaeR7I	CTCGAG	1 cut(s)	256
PciSI	GCTCTTC	2 cut(s)	1830, 2240
PdmI	GAANNNNTTC	2 cut(s)	1722, 1967
PfeI	GAWTC	5 cut(s)	85, 276, 316, 445, 467
PflFI	GACNNNGTC	1 cut(s)	175
PkrI	GCNGC	1 cut(s)	2188
PleI	GAGTC	2 cut(s)	116, 145
PpsI	GAGTC	2 cut(s)	116, 145
PshBI	ATTAAT	1 cut(s)	335
Psp6I	CCWGG	2 cut(s)	192, 1693
PspEI	GGTNACC	1 cut(s)	1330
PspGI	CCWGG	2 cut(s)	192, 1693
PspN4I	GGNNCC	1 cut(s)	1827
PspPI	GGNCC	2 cut(s)	177, 1347
PstNI	CAGNNNCTG	1 cut(s)	956
PsuI	RGATCY	4 cut(s)	226, 918, 1912, 2195
PsyI	GACNNNGTC	1 cut(s)	175
PvuII	CAGCTG	1 cut(s)	650
RsaI	GTAC	7 cut(s)	235, 410, 827, 878, 1012, 1616, 1901
RsaNI	GTAC	7 cut(s)	234, 409, 826, 877, 1011, 1615, 1900
RseI	CAYNNNNRTG	5 cut(s)	599, 812, 1228, 1310, 1568
SalI	GTCGAC	1 cut(s)	423
SapI	GCTCTTC	2 cut(s)	1830, 2240
SaqAI	TTAA	6 cut(s)	335, 348, 531, 1508, 1875, 2042
SatI	GCNGC	1 cut(s)	2187
Sau96I	GGNCC	2 cut(s)	177, 1347
ScaI	AGTACT	1 cut(s)	1616
SchI	GAGTC	2 cut(s)	116, 146
ScrFI	CCNGG	2 cut(s)	194, 1695
SduI	GDGCHC	3 cut(s)	263, 1257, 1866
SfaNI	GCATC	5 cut(s)	530, 642, 669, 1169, 1740
SfcI	CTRYAG	3 cut(s)	6, 769, 1035
Sfr274I	CTCGAG	1 cut(s)	256
SinI	GGWCC	2 cut(s)	177, 1347
SlaI	CTCGAG	1 cut(s)	256
SmiMI	CAYNNNNRTG	5 cut(s)	599, 812, 1228, 1310, 1568
SmlI	CTYRAG	1 cut(s)	256
SmoI	CTYRAG	1 cut(s)	256
SpeI	ACTAGT	1 cut(s)	91
SphI	GCATGC	2 cut(s)	1082, 2299
SsiI	CCGC	1 cut(s)	1852
SspMI	CTAG	3 cut(s)	92, 501, 1373
StyD4I	CCNGG	2 cut(s)	192, 1693
StyI	CCWWGG	7 cut(s)	126, 162, 600, 925, 1335, 1534, 1866
TaaI	ACNGT	7 cut(s)	31, 328, 408, 551, 773, 876, 991
TaiI	ACGT	4 cut(s)	176, 511, 677, 1767
TaqI	TCGA	6 cut(s)	135, 257, 424, 994, 1606, 1885
TatI	WGTACW	4 cut(s)	408, 825, 1010, 1614
TfiI	GAWTC	5 cut(s)	85, 276, 316, 445, 467
Tru1I	TTAA	6 cut(s)	335, 348, 531, 1508, 1875, 2042
Tru9I	TTAA	6 cut(s)	335, 348, 531, 1508, 1875, 2042
TscAI	CASTG	5 cut(s)	34, 411, 546, 994, 1389
TseFI	GTSAC	3 cut(s)	328, 551, 1330
TseI	GCWGC	1 cut(s)	2186
Tsp45I	GTSAC	3 cut(s)	328, 551, 1330
TspGWI	ACGGA	2 cut(s)	169, 913
TspRI	CASTG	5 cut(s)	34, 411, 546, 994, 1389
Tth111I	GACNNNGTC	1 cut(s)	175
VneI	GTGCAC	1 cut(s)	1253
VpaK11BI	GGWCC	2 cut(s)	177, 1347
VspI	ATTAAT	1 cut(s)	335
XapI	RAATTY	6 cut(s)	481, 839, 1679, 2072, 2214, 2280
XceI	RCATGY	3 cut(s)	1017, 1082, 2299
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1570
XhoI	CTCGAG	1 cut(s)	256
XmiI	GTMKAC	2 cut(s)	424, 1907
XmnI	GAANNNNTTC	2 cut(s)	1722, 1967
XspI	CTAG	3 cut(s)	92, 501, 1373
ZraI	GACGTC	1 cut(s)	509
ZrmI	AGTACT	1 cut(s)	1616
Zsp2I	ATGCAT	1 cut(s)	947

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.