Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001609

Physical Location & Seq

Genomic Coordinates Reverse (-)

51754822 .. 51758934

Gene Structure 4113 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G031570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGGCTACAGATGTAATACTGCAAAACACTGTGGAAGAGGAGGAAAGCCAGCATGGGGATCAAGTCTCTGAACAGCTCAAAGAGAATCAACTAGTTTCAAGCCAAAAGTTTCAAAGATTTGACTCCTTGGACATCGAGTCTGGCGCTGATCACAGGCACTCCAAGGAAGTGACGTGGTCCGTAATACTGACCCTGGCATTTCAGAGCATTGGAGTTGTATATGGAGATCTTGGTACTTCACCACTTTATGTGTTCTCGAGCACCTTCCCCAATGGAATCAAAGATAAAGATGACATCTTGGGTGTTCTTTCTTTGATTCTTTATACAGTCACATTAATCCCCTTGATTAAGTATGTTCTCATCGTCCTACAAGCAAATGATAACGGCGATGGAGGGACATTTGCACTGTACTCTCTCTTATGTCGACACGCAAAGATTAGTTTGATTCCGAGCCAGCAAGAAGAGGATTCCAATGTTTCAAATTTCAAACTTGAAGTTCCTAGCAGACGTCTAAAAATGGCATCAACTCTTAAATCTGCACTGGAGAACAGTCACTTTGCCAAAGTCTTCTTACTCTTTGCCACAATGCTTGGCACTTCCATGCTCATTGGCGATGGTGTCCTCACTCCTTGCATCTCAGTTCTATCAGCTGTGGTTGGCATCAAGGGAGCTACGTCTGCAATGACAGAAGATATGATTGTGTGGATATCGGTAGCCATCTTGATAGTCCTTTTCATCATCCAAAGATTTGGAACTGACAAAGTGGGCTACAGTTTTGCTCCAATACTTTGTATTTGGTTTCTATTCATTGGTGTGATCGGCATGTACAATTTCATCAAATTTGATCCAATGGTTATCAAAGCCATAAATCCACGATACATAGTGGATTACTTCATACGGAACAAGAAAGAAGCTTGGATCTCCCTTGGTGGCGTTATTCTATGCATAACAGGAGCTGAAGCTCTGTTTGCTGATGTTGGCCACTTCACTGTTCGATCCATACAAATTATGTTGGCTTACATTGGGCAAGCCTCTTTTCTTCGCAAGCATGCCGATCTTGTTGCCGATACCTTCTTCAAGTCTATACCAAGACCTATATATTGGCCTATGTTTGTTGTTGCTGTATTAGCATCAATAATAGCAAGTCAATCCCTGATTTCTGCGACATTTTCCATAATTCAACAATCTCTGTCATTAGGGTGTTTCCCTCGTGTGAAAGTCGTGCACACATCATCCAAGTATGAAGGACAAGTTTATATTCCGGAGGTCAACTTCATCCTTATGTTGGCCTGTGTAGGGGTCACCTTGGCTTTTAGGACCACTACAAAGATTGGAAATGCACTAGGTATAGCAGTGGTGTTTGTAATGACACTAACTTCATCATTCCTCGTGCTAATTATGATAATGATATGGAAAACCCACATACTTCTCATTATCTCATATGTTCTTATTATTGGAAGTATGGAGCTTCTGTATTTAAGTTCAGTCCTTTACAAGTTTGACCAAGGGGGATATCTTCCAGTCGCCTTTGCCACTCTAATGATGGCTATAATGTTTGTTTGGAATGATGTTTATCGAAGAAAGTACTACTATGAGGTTGAACAGAAAATATCTCCTCAAAAGTTCAAGCAGATTGCTGATGATAGAAAATTTTGTCATATCCCTGGCCTCGCCATATTCTACTCGGAACTTGTTCATGGCATCCCACCCATTTTCAATCACTACTTAGCCAACGTGCCTGCATTGCACTCAGTCCTTGTTTTTGTCTCCATCAGACATTTACCCATAAGCAAGGTTCCGATGGAAGAGCGTCTCCTCTTCCGCCGAGTAGAGCCCAAGGAGCTTAATGTATTTCGATGTGTTGCGAGGTACGGGTATACAGATCTCTACAATGTGCAAGAACCCTTTGAAGAATTGTTGGTAAAAAAGTTGAAAGAGTTCTTGAAAGATGGTTGTTTCATACCCCAACAAACAAGTAATAATGGAGAGGTGTTTGAAAAGCAAGAAGAATTAAATGATGGATTGGTTGACAACGAGAATGGATTTGTTGACATACAACAAGAAGAACTGATGGAGGCTGAGGTTCAACTTGAAGCAATAGATAAAGCATGGAGTTCAGGGGTTATTCACCTCATTGGTGAAAATGAAGTTATAGCTGCCAAAGGATCTGGTATAGCCAAAAGAATTTTGATTGATTATGCTTACAATTTTTTGAAGAGCAATTTGAGGCAGAGAGGCGAATTGTTTGAAATTCCTCGCAAGCGCATGCTAAAAGTGGGTATGACCTATGAACTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

765

Amino Acids

86.15

Weight (kDa)

6.24

Isoelectric Point (pI)

31.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	65 - 542	1.3e-153	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	555 - 765	2.4e-28	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001609)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30541 FvH4_6g30550 FvH4_c6g00050 FvH4_c6g00050 FvH4_c6g00050
malus_domestica	MD03G1283500.v1.1 MD03G1283600.v1.1 MD10G1230200.v1.1 MD10G1230300.v1.1 MD11G1302600.v1.1 MD11G1302900.v1.1 MD11G1303100.v1.1
prunus_persica	Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1
pyrus_communis	pycom03g22450 pycom03g22460
rosa_chinensis	RchiOBHm_Chr2g0136981 RchiOBHm_Chr5g0020481 RchiOBHm_Chr6g0293251
rosa_laevigata	RLG00000011964 RLG00000033905
rosa_multiflora	Rmu_co8314359.1_g000001 Rmu_sc0001374.1_g000063 Rmu_sc0001374.1_g000064 Rmu_sc0021490.1_g000001 Rmu_ssc0000162.1_g000030
rosa_roxburghii	Rroxscaffold_2G00107800 Rroxscaffold_2G00107830 Rroxscaffold_7G00174310
rosa_rugosa	Rorug02G0336400 Rorug06G0233800 Rorug06G0233800 Rorug06G0233800
rosa_samantha	Rh2AG385800 Rh2BG392700 Rh2CG373000 Rh2DG047100 Rh2DG408800 Rh2DG408900 Rh3DG241600 Rh3DG283500 Rh6AG346700 Rh6BG354100 Rh6CG360600 Rh6DG347100
rosa_wichuraiana	Rw2G003360 Rw2G031570 Rw6G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	511
AccI	GTMKAC	2 cut(s)	424, 1877
AccIII	TCCGGA	1 cut(s)	1261
AciI	CCGC	1 cut(s)	1822
AclWI	GGATC	5 cut(s)	66, 839, 926, 990, 2173
AcoI	YGGCCR	1 cut(s)	979
AcsI	RAATTY	5 cut(s)	481, 839, 1649, 2184, 2250
AcuI	CTGAAG	1 cut(s)	978
AcyI	GRCGYC	1 cut(s)	508
AfaI	GTAC	5 cut(s)	235, 410, 827, 1586, 1871
AfiI	CCNNNNNNNGG	3 cut(s)	55, 1285, 1296
AhdI	GACNNNNNGTC	1 cut(s)	136
AhlI	ACTAGT	1 cut(s)	91
AjiI	CACGTC	1 cut(s)	174
AjnI	CCWGG	2 cut(s)	192, 1663
AluBI	AGCT	9 cut(s)	76, 650, 671, 914, 956, 962, 1468, 1843, 2156
AluI	AGCT	9 cut(s)	76, 650, 671, 914, 956, 962, 1468, 1843, 2156
Alw21I	GWGCWC	2 cut(s)	263, 1227
Alw26I	GTCTC	3 cut(s)	70, 1771, 1817
Alw44I	GTGCAC	1 cut(s)	1223
AlwI	GGATC	5 cut(s)	66, 839, 926, 990, 2173
AlwNI	CAGNNNCTG	1 cut(s)	956
Ama87I	CYCGRG	1 cut(s)	256
Aor13HI	TCCGGA	1 cut(s)	1261
AoxI	GGCC	4 cut(s)	979, 1103, 1287, 1666
ApaLI	GTGCAC	1 cut(s)	1223
ApeKI	GCWGC	1 cut(s)	2156
ApoI	RAATTY	5 cut(s)	481, 839, 1649, 2184, 2250
AseI	ATTAAT	1 cut(s)	335
Asp700I	GAANNNNTTC	2 cut(s)	1692, 1937
AspLEI	GCGC	2 cut(s)	146, 2265
AspS9I	GGNCC	2 cut(s)	177, 1317
AsuHPI	GGTGA	4 cut(s)	231, 1294, 2120, 2150
AvaI	CYCGRG	1 cut(s)	256
AvaII	GGWCC	2 cut(s)	177, 1317
BaeGI	GKGCMC	1 cut(s)	1227
BalI	TGGCCA	1 cut(s)	981
BanII	GRGCYC	1 cut(s)	1836
BauI	CACGAG	2 cut(s)	1209, 1388
BbsI	GAAGAC	1 cut(s)	559
Bbv12I	GWGCWC	2 cut(s)	263, 1227
BbvCI	CCTCAGC	1 cut(s)	2079
BbvI	GCAGC	1 cut(s)	2143
BccI	CCATC	9 cut(s)	383, 608, 725, 1537, 1778, 1795, 1943, 2012, 2065
BceAI	ACGGC	1 cut(s)	400
BcgI	CGANNNNNNTGC	2 cut(s)	1043, 1077
BciT130I	CCWGG	2 cut(s)	194, 1665
BclI	TGATCA	1 cut(s)	148
BcoDI	GTCTC	3 cut(s)	70, 1771, 1817
BcuI	ACTAGT	1 cut(s)	91
BfaI	CTAG	3 cut(s)	92, 501, 1343
BfmI	CTRYAG	2 cut(s)	6, 769
BfoI	RGCGCY	1 cut(s)	147
BglII	AGATCT	2 cut(s)	226, 1882
BisI	GCNGC	1 cut(s)	2157
BlsI	GCNGC	1 cut(s)	2158
BmcAI	AGTACT	1 cut(s)	1586
Bme1390I	CCNGG	2 cut(s)	194, 1665
Bme18I	GGWCC	2 cut(s)	177, 1317
BmeRI	GACNNNNNGTC	1 cut(s)	136
BmeT110I	CYCGRG	1 cut(s)	256
BmgBI	CACGTC	1 cut(s)	174
BmgT120I	GGNCC	2 cut(s)	177, 1317
BmiI	GGNNCC	1 cut(s)	1797
BmrFI	CCNGG	2 cut(s)	194, 1665
BmsI	GCATC	5 cut(s)	530, 642, 669, 1139, 1710
BpiI	GAAGAC	1 cut(s)	559
BpmI	CTGGAG	1 cut(s)	563
Bpu10I	CCTNAGC	1 cut(s)	2079
BsaBI	GATNNNNATC	1 cut(s)	1572
BsaHI	GRCGYC	1 cut(s)	508
BsaJI	CCNNGG	8 cut(s)	126, 162, 192, 925, 1305, 1504, 1663, 1836
BsaWI	WCCGGW	1 cut(s)	1261
BsaXI	ACNNNNNCTCC	4 cut(s)	143, 173, 1457, 1487
Bsc4I	CCNNNNNNNGG	3 cut(s)	55, 1285, 1296
Bse1I	ACTGG	2 cut(s)	546, 1520
Bse3DI	GCAATG	2 cut(s)	687, 1742
Bse8I	GATNNNNATC	1 cut(s)	1572
BseAI	TCCGGA	1 cut(s)	1261
BseBI	CCWGG	2 cut(s)	194, 1665
BseDI	CCNNGG	8 cut(s)	126, 162, 192, 925, 1305, 1504, 1663, 1836
BseGI	GGATG	4 cut(s)	738, 1232, 1275, 1701
BseJI	GATNNNNATC	1 cut(s)	1572
BseLI	CCNNNNNNNGG	3 cut(s)	55, 1285, 1296
BseMI	GCAATG	2 cut(s)	687, 1742
BseMII	CTCAG	3 cut(s)	651, 1764, 2070
BseNI	ACTGG	2 cut(s)	546, 1520
BseRI	GAGGAG	3 cut(s)	53, 1605, 1805
BseSI	GKGCMC	1 cut(s)	1227
BseXI	GCAGC	1 cut(s)	2143
BsgI	GTGCAG	1 cut(s)	522
BshFI	GGCC	4 cut(s)	981, 1105, 1289, 1668
BsiHKAI	GWGCWC	2 cut(s)	263, 1227
BsiHKCI	CYCGRG	1 cut(s)	256
BsiSI	CCGG	1 cut(s)	1262
BslFI	GGGAC	1 cut(s)	409
BslI	CCNNNNNNNGG	3 cut(s)	55, 1285, 1296
BsmAI	GTCTC	3 cut(s)	70, 1771, 1817
BsmBI	CGTCTC	1 cut(s)	1817
BsmFI	GGGAC	1 cut(s)	409
BsnI	GGCC	4 cut(s)	981, 1105, 1289, 1668
BsoBI	CYCGRG	1 cut(s)	256
Bsp1286I	GDGCHC	3 cut(s)	263, 1227, 1836
Bsp13I	TCCGGA	1 cut(s)	1261
Bsp1407I	TGTACA	1 cut(s)	825
BspACI	CCGC	1 cut(s)	1822
BspANI	GGCC	4 cut(s)	981, 1105, 1289, 1668
BspCNI	CTCAG	3 cut(s)	650, 1763, 2071
BspEI	TCCGGA	1 cut(s)	1261
BspLI	GGNNCC	1 cut(s)	1797
BspPI	GGATC	5 cut(s)	66, 839, 926, 990, 2173
BspQI	GCTCTTC	2 cut(s)	1800, 2210
BsrDI	GCAATG	2 cut(s)	687, 1742
BsrGI	TGTACA	1 cut(s)	825
BsrI	ACTGG	2 cut(s)	546, 1520
BssECI	CCNNGG	8 cut(s)	126, 162, 192, 925, 1305, 1504, 1663, 1836
BssNAI	GTATAC	1 cut(s)	1878
BssNI	GRCGYC	1 cut(s)	508
BssSI	CACGAG	2 cut(s)	1209, 1388
BssT1I	CCWWGG	6 cut(s)	126, 162, 925, 1305, 1504, 1836
Bst1107I	GTATAC	1 cut(s)	1878
Bst2BI	CACGAG	2 cut(s)	1209, 1388
Bst2UI	CCWGG	2 cut(s)	194, 1665
Bst4CI	ACNGT	6 cut(s)	31, 328, 408, 551, 773, 991
Bst6I	CTCTTC	5 cut(s)	30, 456, 1800, 1823, 2210
BstACI	GRCGYC	1 cut(s)	508
BstAUI	TGTACA	1 cut(s)	825
BstC8I	GCNNGC	8 cut(s)	50, 455, 1029, 1046, 1050, 1740, 2261, 2267
BstDEI	CTNAG	4 cut(s)	637, 1726, 1750, 2079
BstEII	GGTNACC	1 cut(s)	1300
BstF5I	GGATG	4 cut(s)	738, 1232, 1275, 1701
BstH2I	RGCGCY	1 cut(s)	147
BstHHI	GCGC	2 cut(s)	146, 2265
BstMAI	GTCTC	3 cut(s)	70, 1771, 1817
BstMWI	GCNNNNNNNGC	5 cut(s)	677, 968, 1127, 1744, 1840
BstNI	CCWGG	2 cut(s)	194, 1665
BstNSI	RCATGY	3 cut(s)	826, 1052, 2269
BstPI	GGTNACC	1 cut(s)	1300
BstSCI	CCNGG	2 cut(s)	192, 1663
BstSFI	CTRYAG	2 cut(s)	6, 769
BstSLI	GKGCMC	1 cut(s)	1227
BstV1I	GCAGC	1 cut(s)	2143
BstV2I	GAAGAC	1 cut(s)	559
BstX2I	RGATCY	4 cut(s)	226, 918, 1882, 2165
BstXI	CCANNNNNNTGG	1 cut(s)	749
BstYI	RGATCY	4 cut(s)	226, 918, 1882, 2165
BstZ17I	GTATAC	1 cut(s)	1878
BsuRI	GGCC	4 cut(s)	981, 1105, 1289, 1668
BtgZI	GCGATG	2 cut(s)	402, 627
BtrI	CACGTC	1 cut(s)	174
BtsCI	GGATG	4 cut(s)	738, 1232, 1275, 1701
BtsI	GCAGTG	1 cut(s)	1359
BtsIMutI	CAGTG	5 cut(s)	27, 404, 539, 987, 1359
Cac8I	GCNNGC	8 cut(s)	50, 455, 1029, 1046, 1050, 1740, 2261, 2267
CaiI	CAGNNNCTG	1 cut(s)	956
CfoI	GCGC	2 cut(s)	146, 2265
Cfr13I	GGNCC	2 cut(s)	177, 1317
CseI	GACGC	1 cut(s)	1799
Csp6I	GTAC	5 cut(s)	234, 409, 826, 1585, 1870
CspCI	CAANNNNNGTGG	2 cut(s)	571, 606
CviAII	CATG	7 cut(s)	53, 601, 823, 1049, 1697, 2109, 2266
CviQI	GTAC	5 cut(s)	234, 409, 826, 1585, 1870
DdeI	CTNAG	4 cut(s)	637, 1726, 1750, 2079
DriI	GACNNNNNGTC	1 cut(s)	136
EaeI	YGGCCR	1 cut(s)	979
Eam1104I	CTCTTC	5 cut(s)	30, 456, 1800, 1823, 2210
Eam1105I	GACNNNNNGTC	1 cut(s)	136
EarI	CTCTTC	5 cut(s)	30, 456, 1800, 1823, 2210
EciI	GGCGGA	1 cut(s)	1811
Eco130I	CCWWGG	6 cut(s)	126, 162, 925, 1305, 1504, 1836
Eco24I	GRGCYC	1 cut(s)	1836
Eco32I	GATATC	2 cut(s)	708, 1514
Eco47I	GGWCC	2 cut(s)	177, 1317
Eco57I	CTGAAG	1 cut(s)	978
Eco88I	CYCGRG	1 cut(s)	256
Eco91I	GGTNACC	1 cut(s)	1300
EcoO65I	GGTNACC	1 cut(s)	1300
EcoRII	CCWGG	2 cut(s)	192, 1663
EcoRV	GATATC	2 cut(s)	708, 1514
EcoT14I	CCWWGG	6 cut(s)	126, 162, 925, 1305, 1504, 1836
EcoT22I	ATGCAT	1 cut(s)	947
EcoT38I	GRGCYC	1 cut(s)	1836
ErhI	CCWWGG	6 cut(s)	126, 162, 925, 1305, 1504, 1836
Esp3I	CGTCTC	1 cut(s)	1817
FaeI	CATG	7 cut(s)	56, 604, 826, 1052, 1700, 2112, 2269
FaqI	GGGAC	1 cut(s)	409
FatI	CATG	7 cut(s)	52, 600, 822, 1048, 1696, 2108, 2265
FauNDI	CATATG	1 cut(s)	1441
FbaI	TGATCA	1 cut(s)	148
FblI	GTMKAC	2 cut(s)	424, 1877
Fnu4HI	GCNGC	1 cut(s)	2157
FokI	GGATG	4 cut(s)	725, 1219, 1262, 1688
FriOI	GRGCYC	1 cut(s)	1836
Fsp4HI	GCNGC	1 cut(s)	2157
FspBI	CTAG	3 cut(s)	92, 501, 1343
GlaI	GCGC	2 cut(s)	145, 2264
GluI	GCNGC	1 cut(s)	2157
GsuI	CTGGAG	1 cut(s)	563
HaeII	RGCGCY	1 cut(s)	147
HaeIII	GGCC	4 cut(s)	981, 1105, 1289, 1668
HapII	CCGG	1 cut(s)	1262
HgaI	GACGC	1 cut(s)	1799
HhaI	GCGC	2 cut(s)	146, 2265
Hin1I	GRCGYC	1 cut(s)	508
Hin1II	CATG	7 cut(s)	56, 604, 826, 1052, 1700, 2112, 2269
Hin6I	GCGC	2 cut(s)	144, 2263
HinP1I	GCGC	2 cut(s)	144, 2263
HincII	GTYRAC	4 cut(s)	425, 1270, 2029, 2050
HindII	GTYRAC	4 cut(s)	425, 1270, 2029, 2050
HindIII	AAGCTT	1 cut(s)	912
HinfI	GANTC	7 cut(s)	85, 122, 137, 276, 316, 445, 467
HpaII	CCGG	1 cut(s)	1262
HphI	GGTGA	4 cut(s)	231, 1294, 2120, 2150
Hpy166II	GTNNAC	6 cut(s)	425, 1225, 1270, 1878, 2029, 2050
Hpy188I	TCNGA	6 cut(s)	70, 204, 450, 1687, 1775, 1800
Hpy188III	TCNNGA	4 cut(s)	256, 721, 1262, 1942
Hpy8I	GTNNAC	6 cut(s)	425, 1225, 1270, 1878, 2029, 2050
HpyAV	CCTTC	3 cut(s)	274, 1081, 1238
HpyCH4III	ACNGT	6 cut(s)	31, 328, 408, 551, 773, 991
HpyCH4IV	ACGT	4 cut(s)	173, 508, 674, 1734
HpyF10VI	GCNNNNNNNGC	5 cut(s)	677, 968, 1127, 1744, 1840
HpyF3I	CTNAG	4 cut(s)	637, 1726, 1750, 2079
HpySE526I	ACGT	4 cut(s)	173, 508, 674, 1734
Hsp92I	GRCGYC	1 cut(s)	508
Hsp92II	CATG	7 cut(s)	56, 604, 826, 1052, 1700, 2112, 2269
HspAI	GCGC	2 cut(s)	144, 2263
Kpn2I	TCCGGA	1 cut(s)	1261
Ksp22I	TGATCA	1 cut(s)	148
LguI	GCTCTTC	2 cut(s)	1800, 2210
LmnI	GCTCC	5 cut(s)	668, 784, 953, 1465, 1840
Lsp1109I	GCAGC	1 cut(s)	2143
LweI	GCATC	5 cut(s)	530, 642, 669, 1139, 1710
MaeI	CTAG	3 cut(s)	92, 501, 1343
MaeII	ACGT	4 cut(s)	173, 508, 674, 1734
MaeIII	GTNAC	4 cut(s)	169, 328, 551, 1300
MflI	RGATCY	4 cut(s)	226, 918, 1882, 2165
MhlI	GDGCHC	3 cut(s)	263, 1227, 1836
MlsI	TGGCCA	1 cut(s)	981
MluNI	TGGCCA	1 cut(s)	981
MlyI	GAGTC	2 cut(s)	116, 146
Mox20I	TGGCCA	1 cut(s)	981
Mph1103I	ATGCAT	1 cut(s)	947
MroI	TCCGGA	1 cut(s)	1261
MroXI	GAANNNNTTC	2 cut(s)	1692, 1937
MscI	TGGCCA	1 cut(s)	981
MseI	TTAA	6 cut(s)	335, 348, 531, 1478, 1845, 2012
MslI	CAYNNNNRTG	5 cut(s)	599, 812, 1198, 1280, 1538
Msp20I	TGGCCA	1 cut(s)	981
MspA1I	CMGCKG	1 cut(s)	650
MspI	CCGG	1 cut(s)	1262
MspR9I	CCNGG	2 cut(s)	194, 1665
MvaI	CCWGG	2 cut(s)	194, 1665
MwoI	GCNNNNNNNGC	5 cut(s)	677, 968, 1127, 1744, 1840
NdeI	CATATG	1 cut(s)	1441
NlaIII	CATG	7 cut(s)	56, 604, 826, 1052, 1700, 2112, 2269
NlaIV	GGNNCC	1 cut(s)	1797
NmeAIII	GCCGAG	1 cut(s)	1850
NmuCI	GTSAC	4 cut(s)	169, 328, 551, 1300
NsiI	ATGCAT	1 cut(s)	947
NspI	RCATGY	3 cut(s)	826, 1052, 2269
PaeI	GCATGC	2 cut(s)	1052, 2269
PaeR7I	CTCGAG	1 cut(s)	256
PciSI	GCTCTTC	2 cut(s)	1800, 2210
PdmI	GAANNNNTTC	2 cut(s)	1692, 1937
PfeI	GAWTC	5 cut(s)	85, 276, 316, 445, 467
PflFI	GACNNNGTC	1 cut(s)	175
PkrI	GCNGC	1 cut(s)	2158
PleI	GAGTC	2 cut(s)	116, 145
PpsI	GAGTC	2 cut(s)	116, 145
PshBI	ATTAAT	1 cut(s)	335
Psp6I	CCWGG	2 cut(s)	192, 1663
PspEI	GGTNACC	1 cut(s)	1300
PspGI	CCWGG	2 cut(s)	192, 1663
PspN4I	GGNNCC	1 cut(s)	1797
PspPI	GGNCC	2 cut(s)	177, 1317
PstNI	CAGNNNCTG	1 cut(s)	956
PsuI	RGATCY	4 cut(s)	226, 918, 1882, 2165
PsyI	GACNNNGTC	1 cut(s)	175
PvuII	CAGCTG	1 cut(s)	650
RsaI	GTAC	5 cut(s)	235, 410, 827, 1586, 1871
RsaNI	GTAC	5 cut(s)	234, 409, 826, 1585, 1870
RseI	CAYNNNNRTG	5 cut(s)	599, 812, 1198, 1280, 1538
SalI	GTCGAC	1 cut(s)	423
SapI	GCTCTTC	2 cut(s)	1800, 2210
SaqAI	TTAA	6 cut(s)	335, 348, 531, 1478, 1845, 2012
SatI	GCNGC	1 cut(s)	2157
Sau96I	GGNCC	2 cut(s)	177, 1317
ScaI	AGTACT	1 cut(s)	1586
SchI	GAGTC	2 cut(s)	116, 146
ScrFI	CCNGG	2 cut(s)	194, 1665
SduI	GDGCHC	3 cut(s)	263, 1227, 1836
SfaNI	GCATC	5 cut(s)	530, 642, 669, 1139, 1710
SfcI	CTRYAG	2 cut(s)	6, 769
Sfr274I	CTCGAG	1 cut(s)	256
SinI	GGWCC	2 cut(s)	177, 1317
SlaI	CTCGAG	1 cut(s)	256
SmiMI	CAYNNNNRTG	5 cut(s)	599, 812, 1198, 1280, 1538
SmlI	CTYRAG	1 cut(s)	256
SmoI	CTYRAG	1 cut(s)	256
SpeI	ACTAGT	1 cut(s)	91
SphI	GCATGC	2 cut(s)	1052, 2269
SsiI	CCGC	1 cut(s)	1822
SspMI	CTAG	3 cut(s)	92, 501, 1343
StyD4I	CCNGG	2 cut(s)	192, 1663
StyI	CCWWGG	6 cut(s)	126, 162, 925, 1305, 1504, 1836
TaaI	ACNGT	6 cut(s)	31, 328, 408, 551, 773, 991
TaiI	ACGT	4 cut(s)	176, 511, 677, 1737
TaqI	TCGA	6 cut(s)	135, 257, 424, 994, 1576, 1855
TatI	WGTACW	3 cut(s)	408, 825, 1584
TfiI	GAWTC	5 cut(s)	85, 276, 316, 445, 467
Tru1I	TTAA	6 cut(s)	335, 348, 531, 1478, 1845, 2012
Tru9I	TTAA	6 cut(s)	335, 348, 531, 1478, 1845, 2012
TscAI	CASTG	5 cut(s)	34, 411, 546, 994, 1359
TseFI	GTSAC	4 cut(s)	169, 328, 551, 1300
TseI	GCWGC	1 cut(s)	2156
Tsp45I	GTSAC	4 cut(s)	169, 328, 551, 1300
TspGWI	ACGGA	2 cut(s)	169, 913
TspRI	CASTG	5 cut(s)	34, 411, 546, 994, 1359
Tth111I	GACNNNGTC	1 cut(s)	175
VneI	GTGCAC	1 cut(s)	1223
VpaK11BI	GGWCC	2 cut(s)	177, 1317
VspI	ATTAAT	1 cut(s)	335
XapI	RAATTY	5 cut(s)	481, 839, 1649, 2184, 2250
XceI	RCATGY	3 cut(s)	826, 1052, 2269
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1540
XhoI	CTCGAG	1 cut(s)	256
XmiI	GTMKAC	2 cut(s)	424, 1877
XmnI	GAANNNNTTC	2 cut(s)	1692, 1937
XspI	CTAG	3 cut(s)	92, 501, 1343
ZraI	GACGTC	1 cut(s)	509
ZrmI	AGTACT	1 cut(s)	1586
Zsp2I	ATGCAT	1 cut(s)	947

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.